KR20040097716A - Health food comprising the extract of Actinidia arguta for preventing and improving baldness disorders and seborrheic skin disorders - Google Patents

Abstract

PURPOSE: Provided is a functional health food which comprises the extract of Actinidia arguta to prevent and improve depilation and seborrheic skin dermatitis. The extract of Actinidia arguta inhibits the synthesis of dihydrotestos terone in a living body and promotes the generation of hair roots to improve depilation, and suppresses the secretion of sebum to improve seborrheic skin dermatitis. CONSTITUTION: The functional health food for preventing and improving depilation and seborrheic skin dermatitis is characterized by containing the crude or nonpolar solvent soluble extract of fruits, stems, leaves or roots of Actinidia arguta in the amount of 0.01-95 wt.%, and food additives. The crude extract is obtained by using water, lower alcohol or their mixture as an extraction solvent; and the nonpolar solvent soluble extract is obtained by using ethylacetate as an extraction solvent.

Description

Health food comprising the extract of Actinidia arguta for preventing and improving baldness disorders and seborrheic skin disorders}

The present invention relates to a health functional food containing herbal extracts, and more particularly, has the effect of preventing and improving hair loss symptoms and seborrheic skin symptoms containing Actinidia arguta crude extracts, nonpolar solvent soluble extracts or purified fractions. It relates to a functional food.

Alopecia is a common symptom in both men and women, and many people are hampered by their lives, and significant social costs are being spent to treat alopecia and improve their appearance. Normal hair growth follows the cycles of anagen, catagen and telogen. Human hair has a relatively long growth cycle compared to animal hair or other parts of the human body (growth, 2-5 years; degenerate, days-weeks; rest, 3 months). Many factors are involved in controlling the hair growth cycle, but the exact mechanism of action is still known.

There are two main types of alopecia. The first is called male pattern baldness, alopecia seniles, or androgenetic alopecia. It is common in middle-aged men and begins in the frontal head and head government. It is called alopecia areata, and it has a relatively borderless hair loss of round or oval shape and alopecia that can occur in adolescence. Alopecia is not only a disease in men but also occurs in women, and most of the alopecia is masculine alopecia, regardless of gender. Alopecia areata also occurs in both men and women, but women are less frequent than men. (M. Inaba et al., Androgenetic Alopecia, Springer-Berlag, Tokyo, Japan, 1996).

In general, the causes of human hair loss include genetic factors, the progress of aging, drug use, radiation therapy, and post-stress stress. From a biochemical and physiological point of view, hair loss is known to be caused by three major causes of hyperactivity of male hormones, problems with scalp blood circulation, and a lack of nutrients essential for hair metabolism. Although these three factors alone can cause hair loss, when hair loss factors are combined to cause synergy, hair loss is accelerated and symptoms become worse. In particular, if genetic factors or acquired special conditions cause the production and alteration of male hormones, recognition, and signaling overworking, hair follicle tissues tend to age prematurely, which is likely to cause premature baldness. . In addition, due to genetic factors, aging, Western high-fat diet and other external factors, the tension of the scalp is easily increased, or if there are symptoms such as arteriosclerosis, blood circulation disorders occur easily, and thus, there is a problem in smooth hair metabolism.

Among them, the main cause of androgenetic alopecia is known to be related to androgen and steroid hormones (J. Shapiro et al ., Therapeutic agents, Dermatol. Clin ., 16 (2) , pp341-356, 1998). It is also known that differences in the distribution of male hormone receptors can also cause alopecia. The androgen receptor protein is distributed in the connective tissue forming cells (fibroblasts) of the outer root sheath and dermal papilla of the hair, and hair loss occurs in men and women who have caused alopecia. More than 30% were distributed in the frontal head and head government. This means that the signal transduction process to the cell nucleus caused by the binding of the male hormone receptor may affect the dermal papilla and follicular cells, leading to the hair root of the growing phase in the retracted or resting state.

Androgenetic alopecia affects not only male hormones and their receptors, but also enzyme activity. At present, the problem with steroid hormone metabolism related to hair loss is the reductase (5-α-reductase) and aromatase (aromatase). First, the reductase reduces testosterone to dihydrotestosterone (DHT), an enhanced form of male hormone (androgen). Hair loss-related organs include sebaceous gland, epidermis and keratinocytes, dermal papilla cells, sweat gland, and root sheath of the scalp hair follicle. Etc., in particular, the sebaceous gland is attracting attention. Tissues in bald areas are reported to have higher reductase activity than those in normal head areas. Aromatase is known to have low activity in the frontal head of alopecia, which converts male hormone (androgen) to female hormones such as estradiol. Considering this fact, the main cause of androgenetic alopecia may be due to the interrelationship between male hormones, enzymes that modify male hormones, and male hormone receptors.

Alopecia areata is a round or oval with a clear border of hair loss, may be a single or multiple bundles, and in some cases, both the hair and even the eyebrows (external alopecia). Short-lived ones may heal without special treatment, but may recur in a wide range. Recent cell biology studies have shown that alopecia areata is a type of autoimmune disease. In other words, lymphocytes (CD4 +) that can damage the follicles are concentrated around the follicles and the main targets are melanocytes, keratinocytes, dermal papilla, and vascular endothe lium. Is known as

In addition to androgenetic alopecia and alopecia areata, hair loss occurs when various anticancer drugs are administered. When administered to humans and animals, anticancer drugs are the first to act on dividing tissues and cause hair loss as a side effect because they act on cancer tissues, hematopoietic organs, gastrointestinal endothelial cell groups, and sebaceous gland and hair follicle cells of the skin.

5-alpha-reductase inhibitors are used for oral treatment for androgenetic alopecia. Commercially available drugs include finasteride, which has a 4-azasteroid structure, which prevents testosterone from being reduced to more potent DHT, thereby lowering the titer of male hormones. However, the drug relapses when it is discontinued, and cannot be used by women of childbearing potential or pregnant women. Other therapeutic agents include male hormone receptor paralysis, which can only be used in women. Cyproterone acetate, spironola ctone, and estrogen are known to be effective in treating alopecia (J. Shapiro et al. , Hair regrowth, Therapeutic agents, Dermatol. Clin. 16 (2) , 1998). Other therapeutic agents for androgenetic alopecia include many biological response modifiers, such as cell division promoters, vasodilators, capillary development promoters, immunosuppressants, cAMP modulators, epidermal growth factor (EGF), and cytogenicity modulators. There is a minoxidil (Minoxidil). Minoxidil was originally used to treat hypertension as a vasodilator and has been shown to be effective when applied to the hair loss site. However, minoxidil has the side effect of recurring and lowering blood pressure when stopped.

In the treatment of alopecia areata, an immunomodulatory agent is used. Representative drugs are corticosteroids, which act on lymphocytes and are used for the purpose of immunosuppressive effects and are used as subcutaneous injections and ointments.

As discussed above, male alopecia and alopecia areata, as well as there is no treatment in any case, the drugs used can be seen that there are problems such as severe side effects or low effectiveness.

Many attempts have been made to promote hair growth by applying or taking herbal medicines to the scalp, using orchid plant extracts (Korean Patent No. 0157667), and using wormwood and mandrel (Korean Patent No. 0161338). ), In the case of using Manbyeongcho extract as an active ingredient (Korean Patent Publication No. 91-106), hair growth promoter using pine leaf extract (Korean Patent Publication No. 96-40346), hair growth promoter using silkworms (Korea Patent Publication) No. 90-2757), Ogalpi Root, Danner Ginseng Root, Red Sewage Root, Fanzama, and Hair Growth Hair Loss Agent with Buckwheat (Korean Patent Application Laid-Open No. 2000-24499), Half, Clove, Bokbunja, Sancho, Manza, Dansam And when using white porcelain (Korean Patent No. 259037), when using oil obtained by heating Wonjam, latent species, latent fetuses, silkworms, and pupa at about 200 ° C. for about 15 minutes. Heo No. 90-2757), Ginseng, Astragalus, Angelica, Shouo, Hodo, Bokbunja, Raw Yellow Sulfur, Bogolji, Songchae, Dansam, Safflower, Yeosil Chamber Mixed Extract (Korean Patent No. 90-13934) (Silkworm moth), latent species (silkworm seed), pupa (silkworm), black sesame, kelp, sesame, and walnut eggs when used under high pressure and high temperature extraction (Korean Patent Publication No. 90-5952), mung bean flour, soybean flour, Black powder, perilla powder, sesame powder, walnut powder, laver powder, mussel powder, oyster powder, anchovy powder, seaweed powder, kelp powder, yeast concentrate or its dry powder mixture (Korean Patent No. 0260673), casualties, pine needles In the case of using, and Wonjam (Korean Patent Publication No. 90-002757), When using Goji berries, roots, branches (stems), leaves, shoots, flowers, and horseradish beard (Korean Patent Publication No. 2000-36534) The back has been disclosed, but the principle of operation is not clear and the efficacy has not been fully verified.

As described above, the above-mentioned known methods and hair regrowth agents have problems such as economic burdens and pains to hair loss patients or cause side effects, use only temporary or fragmentary pharmacological mechanisms, and the effect is more excellent. And persistent, there is an increasing demand for drugs that do not have side effects.

On the other hand, dihydrotestosterone also has a function of gradually increasing the sebaceous gland attached to the hair roots and promoting sebum secretion.

The sebum secretion due to high sebum secretion is often referred to as seborrheic skin, which is often accompanied by hair loss. Seborrheic skin symptoms can also lead to seborrheic dermatitis, which is not desirable in appearance, such as causing keratin to occur and causing dandruff. Seborrheic dermatitis is a chronic inflammatory disease that occurs on the secretion of sebum, such as the scalp, face, ears, and chest. Red keratin occurs mainly in the 20s and 40s, and the symptoms may vary, but about 3% of adults occur. Acne is also highly associated with seborrheic skin, which is known to occur when the secretion of sebum increases and hair follicles become narrowed or blocked due to the formation of keratin.

Actinidia arguta , the main component of the composition used in the present invention, belongs to Actinidiaceae and is native to Siberia, northern China, and Korea. More than 30 species of similar species have been reported, and the representative ones of which are A. kolomikta , A. polygama , A. rupa , etc., commonly known as kiwi ( A. chinensis or A. deliciosa ) It is used as a Chinese herbal medicine called Mifudo. It contains the fruit of the plant of perilla and related plant, which is cold and nontoxic and has been prescribed for the treatment of liver and gastrointestinal diseases or urinary stones. Drug Database, Institute of Natural Products, Seoul National University, Dongbang Media Co., 1999).

Korean Patent Registration No. 344147 discloses a health beverage mainly based on Tadasu sap and a manufacturing method thereof, and Korean Patent Registration No. 134024 discloses a Health Beverage mainly based on a Tadasu Tadala and a manufacturing method thereof. Patent Application No. 1996-10396 discloses cotton wormwood extract, its liver function protector, and its use as an anticancer agent. Domestic Patent Application No. 2001-17524 discloses beverages for liver function recovery containing wormwood and various herbal extracts. The manufacturing method is disclosed. However, there has been no report on the effects of promoting hair growth and improving seborrheic skin.

Therefore, in the present invention, in order to confirm the hair growth and seborrheic skin symptom improvement effect of the extract, the human body to improve the seborrheic skin symptoms in vivo experiments on the inhibition of dehydrotestosterone production and the promotion of hair growth in the mouse As a result of the test, the present invention was completed by confirming that the worm extract according to the present invention suppresses hair loss and improves seborrheic skin symptoms by lowering the serum level of dihydrotestosterone.

It is an object of the present invention to provide a health functional food useful for the prevention and amelioration of alopecia and seborrheic skin symptoms, containing herbal ingredients with excellent effects, with no side effects predominantly as herbal drugs.

1 is a TLC picture of the silica gel fraction, Figure 1a is a TLC result of the extract and fractions, Figure 1b is a 2D-TLC analysis picture of silica gel fraction 1, Figure 1c is a 2D-TLC analysis of silica gel fraction 2 It is a photograph,

Figure 2 is a diagram showing the result of suppressing serum dehydrotestosterone concentration by the extract of Darae,

3 is a diagram showing the results of suppressing the serum dihydrotestosterone concentration by the non-polar solvent fraction of the Darae hot water extract,

Figure 4 is a diagram showing the results of suppressing serum dihydrotestosterone concentration by silica gel chromatography purified fractions of Darae hot water extract,

FIG. 5 is a photograph showing hair growth on a mouse and the like due to a hair root formation promoting effect induced by treating a herbal composition in a mouse model experiment. FIG. 5A shows hair growth on a mouse and the like after oral administration of drinking water for 2 weeks. It is a photograph, Figure 5b is a photograph taken after the oral administration of the seaweed extract, hair growth.

In order to achieve the above object, the present invention provides a functional food for preventing hair loss and improving hair growth, including a crude extract of Actinidia arguta or a non-polar solvent soluble extract and food supplements.

The present invention also provides a health functional food for preventing and improving seborrheic skin diseases, including a crude extract or a non-polar solvent soluble extract and a food supplement.

The stalks include at least one selected from the group consisting of fruits, stems, stem vines, leaves and roots.

The crude crude extract includes water, lower alcohols such as ethanol, methanol, butanol and extracts soluble in a mixed solvent thereof, preferably in a water or water: ethanol mixed solvent.

The above-mentioned nonpolar solvent soluble extract includes extracts soluble in organic solvents, preferably ethyl acetate, such as chloroform, ethyl acetate, hexane, dichloromethane and ether.

The amount of the stalk extract of the present invention provides a dietary supplement having a weight ratio of 0.01 to 95%, preferably 1 to 80% of the total composition.

The health functional food may include a health food for health, a health beverage, and the like, and may be in the form of powder, granules, tablets, capsules or beverages.

The method of separating the wormwood extract of the present invention is as follows, the present invention will be described in detail below.

Hair extract of the present invention for preventing hair loss, improving hair growth, preventing and improving seborrheic skin disease is characterized in that the extraction with water or alcoholic aqueous solution, more specifically,

The extract of the present invention is dried and pulverized after drying, and then the volume of water, lower alcohols such as methanol, ethanol and butanol, or a drug thereof, in a volume of about 5 to 25 times, preferably about 10 times, the weight of the sputum dry weight. Mixed solvent having a mixing ratio of 1: 0.1 to 1:10, preferably with water, at an extraction temperature of 20 to 100 ° C, preferably 60 to 100 ° C, about 0.5 to 2 days, preferably 1 to 1 After extracting by extraction method such as hot water extraction, cold needle extraction, reflux cooling extraction or ultrasonic extraction for 1 to 5 times, preferably 2 to 3 times consecutively during the day, filtered through a filtrate and the filtrate to a rotary vacuum concentrator After concentration under reduced pressure at 20 to 100 ° C, preferably 50 to 70 ° C, the residue was extracted by smelting of water, lower alcohol, or a mixed solvent thereof through vacuum freeze drying, hot air drying or a drying method by spraying. Can be obtained, the obtained extract of Actinidia is used in the experiments was stored at -20 ℃. In addition, the dry powder of the stalk extract is used by dissolving to a certain concentration in distilled water.

In addition, after the extract of the present invention is suspended in water, it is added 1 to 10 times by adding a nonpolar solvent such as ethyl acetate, chloroform or hexane in a volume of about 1 to 100 times, preferably about 1 to 5 times the suspension, Preferably it can be obtained by fractionation with 2 to 5 separatory funnel. It is also possible to further carry out conventional fractionation processes (Harborne JB Phytochemical methods: A guide to modern techniques of plant analysis . 3rd Ed. Pp 6-7, 1998).

The Darae extract of the present invention is subjected to adsorption chromatography or TLC, such as silica gel column chromatography with the non-polar solvent soluble extract, preferably a mixed solvent of chloroform: methanol: water in a silica gel column 100: 10: 1 (w: A developing solvent having a ratio w: w) to 20: 10: 0.5 can be obtained after fractionation by developing on a column.

Crude crude extract, non-polar solvent soluble extract and column chromatography fraction obtained by the above method inhibit blood dihydrotestosterone concentration when orally administered to mice, and also promote hair follicle formation in mice by orally administering the extract of Tara to mouse hair growth model It was confirmed to promote hair growth through.

In addition, the crude extract was improved oral and keratin symptoms of the skin when orally administered to patients with seborrheic skin.

In addition, the present invention is a first step of obtaining a crude extract soluble in a polar solvent by pulverizing and extracting with a polar solvent such as water, methanol or ethanol and concentrated under reduced pressure;

A second step of dissolving the crude extract in a nonpolar solvent such as ethyl acetate and suspending the mixture, followed by filtration to separate the nonpolar solvent soluble extract layer and concentrate to obtain a nonpolar solvent soluble extract available in the nonpolar solvent;

Subsequently, column chromatography of the non-polar solvent soluble extract of two stages is carried out, and a developing solvent having a chloroform: methanol: water mixed solvent of 100: 10: 1 (w: w: w) to 20: 10: 0.5 ratio in the silica gel column Is purified on a column, and then dried, to obtain purified fractions, which are obtained by a manufacturing method comprising a third step of producing a purified fraction (see FIGS. 1A to 1C) and food additives. It provides a health functional food containing food supplements to prevent hair loss, improve hair growth or improve seborrheic skin diseases.

The herbal extract or tablet fraction of the present invention may be added to food or beverage for the purpose of preventing hair loss and improving hair growth or improving seborrheic skin disease. At this time, the amount of the extract or tablet fraction in the food or beverage may generally be added at 0.01 to 95% by weight, preferably 1 to 80% by weight of the total food weight, the health functional beverage composition is based on 1 100ml It can be added in the ratio of -30 g, Preferably it is 3-10 g.

Foods to which the extract or tablet fraction of the present invention may be added for development for health foods include, for example, various foods, beverages, gums, vitamin complexes, health supplements, and the like, powders, granules, tablets, and capsules. Or in the form of a beverage or the like.

The health functional beverage composition of the present invention has no special limitation except for containing the extract or the purified fraction as an essential ingredient in the indicated ratio, and may contain various flavors or natural carbohydrates as additional ingredients such as ordinary drinks. Can be. Examples of the above-mentioned natural carbohydrates include monosaccharides such as glucose, fructose and the like; Disaccharides such as maltose, sucrose and the like; And conventional sugars such as polysaccharides such as dextrin, cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents other than those described above, natural flavoring agents (tauumatin, stevia extract (e.g., Rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (saccharin, aspartame, etc.) can be advantageously used. The proportion of natural carbohydrates is generally about 1-20 g, preferably about 5-12 g per 100 ml of the composition of the present invention.

The invention also Actinidia extract 0.01 to 20% by weight, amino acids 0.001 to 5% by weight, vitamins 0.001 to 2% by weight, sugars 0.001 to 20% by weight, organic acids 0.001 to 10% by weight, sweetener and flavors of proper amount Provided is a healthy beverage composition for preventing hair loss, improving hair growth, or improving seborrheic skin disease by adding such.

In addition to the above, the composition of the present invention includes various nutrients, vitamins, minerals (electrolytes), flavors such as synthetic flavors and natural flavors, coloring and neutralizing agents (such as cheese and chocolate), pectic acid and salts thereof, alginic acid and salts thereof. , Organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. The compositions of the present invention may also contain pulp for the production of natural fruit juices and fruit juice beverages and vegetable beverages. These components can be used independently or in combination. The proportion of such additives is not so critical but is generally selected from the range of 0 to about 20 parts by weight per 100 parts by weight of the composition of the present invention.

Hereinafter, the present invention will be described in detail by Examples and Experimental Examples.

However, the following Examples and Experimental Examples are only illustrative of the present invention, and the content of the present invention is not limited to the following Examples and Experimental Examples.

Example 1. Preparation of Thigh Extract

(1-1) Hot Water Extraction Method Using Water Solvent

Dried dairae (Mifudo, Actinidia arguta ) was purchased from Chinese herbal medicine in Kyungdong market and used for experiment. 100 g of pulverized bran was added to 1 liter of distilled water, and the mixture was stirred well. The mixture was refluxed for 3 hours at an extraction temperature of 90 to 95 ° C., the filtrate was separated, and the herbal extract was concentrated under reduced pressure at 55 to 65 ° C. After lyophilization, x 15.6 g of herbal composition powder was obtained.

(1-2) Hot Water Extraction Method Using Water-Alcohol Mixed Solvent

1 g of 25% ethanol was added to 100 g of the pulverized sieve as in Example 1-1, followed by stirring. Then, the mixture was heated to reflux for 3 hours at an extraction temperature of 80 to 90 ° C., and the filtrate was separated. The herbal extracts were concentrated under reduced pressure at 55-65 ° C., and then lyophilized to obtain 13.5 g of the herbal composition powder.

Example 2 Preparation of Soluble Extract of Polar and Non-Polar Solvents

The hot water extract prepared in Example was fractionated using an organic solvent.

(2-1) Chloroform Soluble Fraction Separation

5 g of the crude crude extract of Example 1 was dissolved in 50 ml of distilled water, and then mixed with 50 ml of chloroform into a separatory funnel, followed by vigorous mixing to separate the chloroform insoluble layer (aqueous layer) and the chloroform soluble layer. Again, 50 ml of chloroform was added to the chloroform insoluble layer (aqueous layer), followed by mixing and fractionation to collect a chloroform insoluble fraction and a soluble fraction.

(2-2) Separation of ethyl acetate soluble fraction

50 ml of ethyl acetate was added to the chloroform insoluble fraction (aqueous layer), followed by mixing. The ethyl acetate soluble fraction and the insoluble fraction were separated, and 50 ml of ethyl acetate was added to the ethyl acetate insoluble fraction (aqueous layer). Soluble fraction and insoluble fraction (aqueous layer) were collected.

The chloroform soluble fraction, ethyl acetate soluble fraction and the remaining aqueous layer were concentrated under reduced pressure, and then lyophilized to obtain 0.34 g of chloroform fraction, 0.05 g of ethyl acetate fraction, and 4.61 g of water fraction.

Example 3 Botanical Extract Fractions by Silica Gel Column Chromatography

The ethyl acetate soluble extract silica gel column chromatography (Daisogel IR-60-W-40: 63 mm) of Example 2-2 was carried out, and the developing solvent was a chloroform: methanol: water mixed solvent ([1] 90:11). : 1, [2] 60: 10: 1, [3] 60: 20: 2) and methanol [4] were used, which was carried out at 300 mL per hour to obtain 4 additional fractions.

Each fraction obtained from 2,784 mg of ethyl acetate fraction was [1] 2,381 mg, [2] 135 mg, [3] 148 mg, [4] 98 mg.

TLC (TLC plate: Merck, developing solvent: chloroform: methanol: water = 9: 5: 1) was performed using the above-mentioned hot water extract, ethyl acetate soluble extract and each fraction (see FIG. 1A). In Fig. 1a, lane 1 is a stratum hydrothermal extract, lane 2 is a stratum ethyl acetate fraction, lane 3 is a stratum extract silica gel fraction [4], lane 4 is a stratum extract silica gel fraction [3], lane 5 is a stratum extract silica gel fraction [ 2], lane 6 is the Tsu extract silica gel fraction [1].

In addition, 2D-TLC was performed on silica gel fractions [1] and [2]. As the first developing solvent, chloroform: methanol: water = 9: 5: 1 and the second developing solvent were performed using chloroform: acetone: water = 3: 8: 0.5 (see FIGS. 1B and 1C).

Experimental Example 1. Inhibition test of blood dehydrotestosterone by the extract

In order to verify the blood dehydrotestosterone inhibitory effects of the water extract and ethanol extracts of the haejugo obtained in Examples (1-1) and (1-2), the concentration of dehydrotestosterone in the blood Measured. Six week-old C57BL / 6 male mice were purchased and allowed to acclimate to the environment for one week, and then, at the age of seven weeks, 16 animals were divided into four groups and obtained in Examples (1-1) and (1-2) for three weeks. 100 μl (1500 μg / mouse / day) of Dalai extract, respectively, oral administration of finasteride (100 μg / mouse / day) and 100 μl of drinking water were used as control. Serum was isolated from blood collected at the expense of mice after oral administration for 3 weeks, and then the concentration of dihydrotestosterone was determined using ELISA (IBL-Hamburg GmbH).

In the results of Figure 2, when the daily dose of the water extract and ethanol extract 1500 ㎍ per mouse daily, it can be seen that the concentration of serum dihydrotestosterone is suppressed up to 90%, which means that the finasteride 100 per mouse per day It was confirmed that the effect was similar to that obtained when taking ㎍.

Experimental Example 2 Dehydrotestosterone Inhibitory Effect of Soluble Extract of Non-polar Solvent

In the same manner as in Experiment 1, 16 C57BL / 6 male mice were prepared, divided into 4 groups, and the organic solvent fractions prepared in Example 2, that is, the chloroform fraction, the ethyl acetate fraction and the water fraction, were prepared in Example 2 for 3 weeks. After oral administration daily, the concentration of serum dihydrotestosterone was measured. 100 μl of water was administered to the control group.

As a result of measuring the concentration of serum dihydrotestosterone using the ELISA method (see FIG. 3), the concentration of serum dihydrotestosterone was suppressed by 95% or more in the mice treated with the ethyl acetate fraction, and the experimental group in which the water fraction was administered. Also, the concentration of dihydrotestosterone tended to decrease slightly. The chloroform fraction, however, did not affect serum dihydrotestosterone levels.

Experimental Example 3. Dehydrotestosterone inhibition effect by silica gel column chromatography fractionation

In the same manner as in Experiment 1, 16 C57BL / 6 male mice were prepared, divided into 4 groups, and the silica gel column chromatography fractions [1] to [4] obtained in Example 3 were orally administered every 3 weeks for 30 weeks. The concentration of serum dihydrotestosterone was then measured. 100 μl of water was administered to the control group. As shown in FIG. 4, the serum dihydrotestosterone concentration was measured using an ELISA method. As a result, a strong dehydrotestosterone inhibitory effect was observed in the mice treated with the fraction [1] and the fraction [2].

Experimental Example 4. Experiment of hair root formation promoting effect by the extract

(4-1) Promoting Hair Root Formation of Thigh Extract

In order to verify the effect of preventing and treating alopecia due to the aloe hot water extract obtained in Example 1, it was confirmed that the hair root formation promoting effect was generally applied by applying a mouse hair growth model. Six-week-old C57BL / 6 female mice were purchased and allowed to acclimate to the environment for one week. At seven weeks of age, the hairs of the mice and the like were removed using baricang. At this time, since the hair roots of the mice all entered the telogen, the skin was bright red. When hair roots are formed and hair grows, the skin becomes dark due to a large amount of melanin pigment. From the day after hair removal, 10 mice were divided into two groups, and 100 µl of water extract (1500 µg / mouse / day) and 100 µl of drinking water were orally administered for 4 weeks. The activation of hair roots by converting the hair roots in the telogen to anagen can be easily observed through darkening of the skin color that was bright red. In addition, by directly observing the growth of hair, the hair growth promoting effect of the extract was observed.

In the group of mice treated with hot water extract, the skin color became dark before 2 weeks and then hair growth was observed (see FIG. 5B), but no change was observed in the mouse of the control group administered with water. (See FIG. 5A). Also no hair growth was observed. At 4 weeks, the backs of the mice treated with the wormwood were almost covered with fur, but the backs of the control mice were still free of new hairs. Through this experiment, it was confirmed that the Darae extract significantly promoted hair growth by promoting hair follicle formation in the mouse.

(4-2) Enhancement of Hair Root Formation of Ethyl Acetate Fraction from Tela Water Extract

Mouse experiment by the same method as Experimental Example 4-1 in order to confirm the hair root formation promoting effect of the sample obtained by further fractionating the hot water extract of Darae as an organic solvent, that is, the chloroform fraction, ethyl acetate fraction, water fraction as in Example 2 Was carried out. Five mice with hair removed on the back were divided into 4 groups, and 100 μl (50 μg) of water fraction, ethyl acetate fraction, and chloroform fraction were administered and 100 μl of drinking water for 4 weeks each day. As a result, it was observed that hair growth was significantly promoted in the group of mice treated with the ethyl acetate fraction. In other groups, no hair growth was observed.

Experimental Example 5. Inhibitory Effect of Dihydrotestosterone on Hot Water Extract of Twigi Vine

Mouse experiment was conducted in the same manner as in Experiment 1 to confirm the dehydrotestosterone inhibitory effect of the stem of the vines (Thufu, etc.) hydrothermal extract. Eight seven-week-old C57BL / 6 male mice were divided into two groups, and the drinking water and the vine extract (1500 μg / mouse / day) were orally administered for 3 weeks, respectively, and the concentration of serum dihydrotestosterone was measured. Mice taking the vinegar extract showed serum dihydrotestosterone concentrations of 142 pg / ml, 193 pg / ml, 362 pg / ml and 1625 pg / ml. Therefore, it can be seen that the vines of the future (back light) also have a significant inhibitory effect of dihydrotestosterone production.

Experimental Example 6. Experimental effect on seborrheic skin by using hot water extract

Three volunteers with seborrheic skin were taken daily for 1 week for 4 weeks of thigh hot water extract (1 g) and the improvement of seborrheic skin was observed. All three participants reported improvement in the symptoms of skin swelling and keratin formation, including the scalp after two weeks of administration.

Human test results

Applicant 1

Name : Sukyung Kwon (female, 29, Banpojugong Apartment, Gangnam-gu, Seoul)

Symptoms before taking : Scalp and keratin of the scalp, dandruff occurs immediately after washing the hair, a large amount of hair is missing when washing the hair.

After taking : After 2 weeks of treatment, seborrheic symptoms and dead skin cells of scalp were clearly reduced and hair loss was reduced by more than 50%.

Applicant 2

Name : Park Kyung-chul (Male, 27, 1510-2, Bongcheon 7-dong, Gwanak-gu, Seoul)

Symptoms before taking : Face and hair are boring, so even after washing the face, oils get on the face and big dandruff drops off the head frequently.

After taking : The oil is definitely reduced when you touch your face.

Applicant 3

Name : Ee Haegwan (Male, 29 years old, Eunma Apartment Building 20, 104, Daechi 2-dong, Gangnam-gu, Seoul)

Pre-dose symptoms : Seborrheic skin, especially on the neck, especially on the face and scalp. If you don't wash your hair, it's very itchy and you have red spots like acne on your scalp. But the body was dry.

After taking : 2 weeks after taking it, the scalp disappeared and the acne disappeared naturally. When I stopped taking it, I began to develop symptoms similar to the old ones.

Experimental Example 7. Toxicity Test

Mice were used to carry out repeat toxicity test of the extracts. Female Balb / c mice were divided into two groups of ten rats each day, and the dose of 150 mg / kg body weight was administered for 4 weeks, and the control group received water. Signs of toxicity were identified for 4 weeks through changes in body weight, hematological analysis, and biopsy.

As a result of the experiment, the extract of Dalai was not observed at 150 mg / kg of the dose. I could confirm it.

(1) Body weight and visual observation: No abnormal weight change was observed.

(2) Hematology test: any abnormalities in the number of WBCs, lymphocytes, monocytes, neutrophils, eosinophils, basophils, RBCs, hemoglobin, platelets No signs were found.

(3) Serum biochemical test: Biochemical test using serum AST, ALT, LDH, bilirubin, creatinine, glucose, cholesterol, cholesterol, minerals, albumin, BUN Did not show any signs of abnormality in levels of lipase, amylase.

(4) Biopsy: No abnormalities found in the tissues of the kidneys, spleen, liver and thymus.

Hereinafter, an example of the preparation of the health functional food will be described, but the present invention is only intended to be described in detail rather than to limit it.

Formulation Example 1 Preparation of Health Food

Botanical extract of Example 1 ..................... 1000 mg

Vitamin Blend ............... 20 g

Vitamin A Acetate ............... 70 μg

Vitamin E ......................... 1 mg

Vitamin B1 ..................................... 0.13 mg

Vitamin B2 ................................. 0.15 mg

Vitamin B6 ................................. 0.5 mg

Vitamin B12 ................................. 0.2 μg

Vitamin C ................................. 10 mg

Biotin ......................................... 10 μg

Nicotinic Acid Amide ......................................... 1.7 mg

Folic Acid ......................................... 50 ㎍

Calcium Pantothenate ......................................... 0.5 mg

Mineral mixture .............

Ferrous Sulfate ................................. 1.75 mg

Zinc Oxide ........................ 0.82mg

Magnesium Carbonate ............... 25.3 mg

Potassium monophosphate ......................................... 15 mg

Dicalcium Phosphate Dioxide ..................... 55 mg

Potassium citrate ..... 90 mg

Calcium Carbonate ... 100 mg

Magnesium Chloride .............. 24.8 mg

Although the composition ratio of the vitamin and mineral mixture is a composition suitable for a relatively healthy food in a preferred embodiment, the composition ratio may be arbitrarily modified, and the above ingredients are mixed according to a conventional health food manufacturing method, Granules may be prepared and used to prepare health food compositions according to conventional methods.

Formulation Example 2 Preparation of Health Beverage

Botanical extract of Example 1 ...................... 1000 mg

Citric Acid ..................... 100 mg

Oligosaccharide ......................................... 100 g

Plum concentrate ..................... 2 g

Taurine ..................................... 1 g

Purified water is added.

After mixing the above components according to a conventional healthy beverage production method, and then stirred and heated at 85 ℃ for about 1 hour, the resulting solution is filtered and obtained in a sterilized 2 L container sealed sterilization and then refrigerated and stored in the present invention For the preparation of healthy beverage compositions.

Although the composition ratio is mixed with a component suitable for a favorite beverage in a preferred embodiment, the composition ratio may be arbitrarily modified according to regional and ethnic preferences such as demand hierarchy, demand country, and usage.

The composition containing the crude extract, polar solvent and non-polar solvent extract provided by the present invention significantly promotes the formation and hair growth of hair roots, improves the symptoms of seborrheic skin disease, and has no side effects, thereby preventing hair loss and improving hair growth or seborrheic skin. It can be used as a safe and effective dietary supplement for the improvement of disease.

Claims (10)

A dietary supplement for hair loss prevention and hair growth improvement comprising a crude extract or a non-polar solvent soluble extract and a food supplement additive. A dietary supplement for improving seborrheic skin disease, which comprises a crude extract or a non-polar solvent soluble extract and a food supplement. The dietary supplement according to claim 1 or 2, wherein the stalk is at least one of the fruit, stem, stem vine, leaf or root of the tree. The health functional food according to claim 1 or 2, wherein the crude extract is soluble in water, lower alcohol, or a mixed solvent thereof. The health functional food according to claim 1 or 2, wherein the nonpolar solvent soluble extract is soluble in ethyl acetate. The health functional food according to claim 1 or 2, wherein the amount of the extract has a weight ratio of 0.01 to 95% of the total composition. A first step of pulverizing the wormwood and extracting with a polar solvent such as water, methanol or ethanol and then concentrating under reduced pressure to obtain a crude extract soluble in a polar solvent; A second step of dissolving the crude extract in a nonpolar solvent such as ethyl acetate and suspending the mixture, followed by filtration to separate the nonpolar solvent soluble extract layer and concentrate to obtain a nonpolar solvent soluble extract available in the nonpolar solvent; Subsequently, column chromatography of the non-polar solvent soluble extract of two stages is carried out. The purified fractions and foodstuffs as shown in Figs. 1a to 1c obtained by the method comprising the step of developing on a column and then fractionating and drying to obtain a purified fraction in purified form. Health functional food for hair loss prevention, hair growth improvement or seborrheic skin disease improvement containing food supplements which can be added as The health functional food according to any one of claims 1, 2 and 7 in the form of a powder, granule, tablet, capsule or beverage. Actinidia extract 0.01 to 20% by weight, amino acids 0.001 to 5% by weight, vitamins 0.001 to 2% by weight, sugars 0.001 to 20% by weight, organic acids 0.001 to 10% by weight, sweetener and prevent the addition of flavoring hair loss and hair growth Health drink composition for improvement. Of Actinidia extract 0.01 to 20% by weight, amino acids 0.001 to 5% by weight, vitamins 0.001 to 2% by weight, sugars 0.001 to 20% by weight, organic acids 0.001 to 10% by weight, seborrheic the addition of sweetening agents and flavoring skin disease Health drink composition for improvement.