KR20040074667A - Feed additive containing bamboo vinegar - Google Patents

Abstract

PURPOSE: An animal feed supplement containing a bamboo extract, bamboo charcoal, bamboo salt and sulfur is provided. It enhances the immunity of an animal against disease and meat quality such as high protein, low cholesterol, low fat or the like when fed to the animal. CONSTITUTION: The animal feed supplement comprises 0.005 to 0.1 parts by weight of a bamboo extract, 0.1 to 0.3 parts by weight of bamboo charcoal, 0.01 to 0.03 parts by weight of bamboo salt and 0.06 to 0.12 parts by weight of sulfur. In the case of feeding the animal feed supplement to an animal, it contains 500 to 1,000g bamboo extract, 1,000 to 2,000g bamboo charcoal, 100 to 300g bamboo salt and 1,200g sulfur, based on 1,000kg assorted feed(formula feed).

Description

Feed additive containing bamboo vinegar

The present invention relates to a feed additive containing bamboo vinegar, and more particularly to a feed additive consisting of bamboo vinegar, charcoal flour, bamboo salt and sulfur. More specifically, the feed additive of the present invention is formulated to commercially available conventional feeds and is fed to Korean cattle and pigs so that it has high growth potential, high immunity, excellent meat quality, and high protein, low cholesterol and low fat pork compared to general pork. To provide.

By feeding the feed additive of the present invention to a general animal feed and feeding to cattle, pigs, etc., the disease resistance is high, the disease-free livestock raising and meat quality is very soft, but low fat, low cholesterol, and reduced the smell of meat to produce various adult diseases Not only can they prevent meat, but also produce meat with excellent taste, and the beef, pigs, etc., who ate such feeds, have less added odor and emit less livestock waste, resulting in added value that can reduce environmental pollution and disposal costs. It is possible to produce high quality and high functional meat.

Korean beef and pork are livestock foods that are popular with us as a good source of protein. It is a time when the import wave is increasing and the production of livestock products with international competitiveness is urgently needed. Moreover, the dependence on imported feed is increasing and the crisis of livestock farmers caused by various diseases and infectious diseases is getting serious. Accordingly, many livestock farmers are urgently needed to produce high-quality brand meat that is differentiated from imported meat by reducing livestock production cost and quality, and is cheap, high quality and safe and hygienic.

The present invention is mixed with commercially available feed in the feed additives containing bamboo vinegar solution, bamboo charcoal powder, bamboo salt and sulfur in a predetermined ratio to feed the livestock by providing fast growth and high immunity, very meat quality, but high protein compared to normal pork Its purpose is to produce high quality pork with low cholesterol and low fat.

In order to achieve the above object, the present inventors have repeatedly conducted experimental research for a long time, and have developed a feed additive homogeneously mixed with charcoal flour, bamboo salt, and bran oil in bamboo vinegar solution, and adding the feed additive to an existing general compound feed. As a result of feeding livestock, it is possible to produce high-quality pork with high protein, low cholesterol, and low fat compared to general pork, with fast growth, high immunity, excellent meat quality.

The feed additive of the present invention is prepared by mixing homogeneously with 0.05 ~ 0.1 parts by weight bamboo bamboo charcoal powder 0.1. ~ 0.3 parts by weight, 0.01 ~ 0.03 parts by weight bamboo salt and 0.06 ~ 0.12 parts by weight of bamboo salt on the basis of weight parts, the feed additive is It is mixed with commercially available commercial feed and administered to livestock.

Preferred feed additives for livestock feed range from 500g to 1,000g of bamboo vinegar, 1,000g to 2,000g of charcoal powder, 100g to 300g of bamboo salt, and 600g to 1,200g of sulfur based on 1,000Kg (1 ton) of compounded feed. It is preferable to add by.

Bamboo bamboo heat is pyrolyzed when bamboo is heated, leaving smoked smoke and bamboo charcoal. At this time, smoke is a mixture of gas emitted from bamboo by pyrolysis and water vapor containing various components. Refers to a reddish brown transparent liquid prepared by.

The bamboo vinegar solution used in the present invention is purified by distilling the bamboo vinegar solution which cooled and condensed the smoke generated when carbonization of bamboo. It is also known to be effective in stopping diarrhea and suppressing the occurrence of odor in the barn. In addition, when feeding bamboo vinegar to livestock, it is possible to obtain high value-added high-quality meat by improving digestive absorption rate, reducing intestinal gas production, improving gastric digestion rate, improving intestinal microflora, increasing weight of animal (weight gain) and improving intramuscular retention. there was.

Hereinafter, the present invention will be described in detail through the following examples. The embodiments of the present invention are only illustrative examples of the present invention, and the present invention is not limited by the following examples.

Example 1

Preparation of Bamboo Vinegar

Attach the cooling vat about 30cm away from the bamboo charcoal kiln, and attach the hood to collect smoke at the entrance of the chimney. Cooling bins follow the usual method of collecting, cooling and collecting liquid bamboo charcoal smoke using stainless steel pipes. Bamboo is pulverized at 400 ° C to 500 ° C and smoke begins to be collected when the smoke temperature at the chimney inlet reaches 80 ° C to 150 ° C. The color of the smoke is white, and when the flue gas temperature of the chimney entrance is about 200 ℃, blue smoke comes out and the smoke collector is removed and the smoke collection is stopped. The collected bamboo vinegar solution was aged for 6-12 months in an agricultural poly container. Subsequently, the tar component of the upper and lower layers was removed to obtain only the transparent bamboo vinegar solution of the intermediate layer. The obtained bamboo vinegar liquor was filtered using filter paper (nonwoven fabric, etc.) to obtain a transparent light brownish brown vinegar liquor.

As a result of analyzing the components of the bamboo vinegar obtained above, the specific gravity was 4.5, the organic acid content was 9.9%, pH 2.8, dissolved tar content 3.1%, the refractive index was 13.1%. As a result of structural identification and quantification of these bamboo vinegar solutions, acetic acid, 1-hydroxy-2-propanone, methanol, 1-hydroxy-2butanone hydroxyl-2-butanone) and propionic acid were the main compounds, and total organic content was 14.8%. Among these, organic acids (acetic acid, formic acid, propionic acid, butanoic acid) were 9.0%, accounting for about 61% of all organic materials. Phenols and neutrals accounted for about 6% and 31%, respectively, of 0.8% and 4.5%, respectively.

Example 2

Preparation of Feed Additives

500 g of bamboo vinegar solution obtained in Example 1 was mixed with 1,000 g of charcoal flour, 100 g of bamboo salt and 600 g of boiled milk to prepare a feed additive of 2,200 g. The feed additive obtained here is mixed homogeneously with the general compound feed (1,000 kg) to feed the livestock.

Test Example 1

The test group fed 40 pigs of 30-day-old pigs into two groups and fed the feed homogenously mixed with 2,200 g of the feed additive prepared in Example 2 to 1,000 Kg (1 ton) of commercially available general feed. In each test group and the control group, A (n = 20) and two treatment groups of control group B (n = 20), which were fed only the general feed without the feed additive of the present invention, were fed for 6 weeks. The weight gain and feed efficiency of pigs were measured. The test results are as follows.

Feed efficiency and weight gain test in swine division Average weight Feed efficiency Test group A (n = 20) 4.67 0.382 Control group B (n = 20) 3.19 0.307

N is the number of individuals

As shown in Table 1, the average weight of the test group A was 4.67 kg, whereas the average weight of the control group was 3.19 kg, respectively, in the results of weight gain and feed efficiency measurement for 6 weeks after the test. There was a big difference in terms. In addition, the feed efficiency of test group A and test group B was 0.382, respectively, whereas the control efficiency of control group B was 0.307, and the feed efficiency was significantly higher than that of the control group.

※ Feed efficiency = feed intake (kg) / weight increase by 1 kg

Test Example 2

For two treatment groups of 40 30-day-old pigs, the feed additives prepared in Example 2 were mixed with commercially available commercial feed and fed as feed test group A-1 (n = 20). Feeding only the general feed, which was not included, was given to the control group B-1 (n = 20) for 10 weeks or more. The primary porcine cholera vaccine inoculation of test group A-1 and control group B-1 was performed at 5 weeks of age, and the secondary pig cholera vaccine was performed at 9 weeks of age. Each group of the test group A-1 and the control group B-1, which were subjected to the first and second swine cholera vaccinations, was challenged with swine cholera at 11 weeks of age with 2 ml of HCV reading 10 ⁴ TCID ₅₀ . The change in test group A-1 and control group B-1 was measured while feeding more. Immune cell changes were measured before and after swine cholera challenge for each test group A-1 and control group B-1 during the 14-week-old pig period of 30 days of age.

Peripheral blood leukocytes were isolated from each other in group A-1 and control group B-1 by David et al. (1987), followed by leukocyte surface monoclonal antibody and flow cytometry (David et al., 1990). We investigated the effect on the distribution of immune cells, ie, Major Histocompatibility Complex and lymphocyte subpopulations. The test results are as follows.

Piglet cholera immune boosting effect test in swine 1 division Vaccine Antibody Titer ¹ Immune Cell Activity ² MHC-Class II CD4 ⁺ T lymphocytes B Cell Test group A-1 (n = 20) ⁴ after processing 130.0 23.23 ± 6.26 26.15 ± 3.63 12.88 ± 2.38 ³ before processing 4.9 15.55 ± 2.75 30.68 ± 2.65 11.78 ± 0.75 Control group B-1 (n = 20) After treatment 101.3 17.60 ± 0.00 23.50 ± 0.00 11.30 ± 0.00 Before treatment 11.1 14.57 ± 3.18 32.97 ± 0.94 10.45 ± 2.23

1: Measured titer 2 weeks after 2 vaccinations

2,4: 2 weeks after 2 vaccinations after virus challenge

3: immediately after implementation

N is the number of individuals

As shown in Table 2, the vaccine antibody titers of the test group A-1 were 130.0, respectively, which was higher than the average value of the control group B-1, 101, and the cellular immunity of the test group A-1 in the immune cell activity after virus challenge. MHC-class II, CD4 ⁺ and B lymphocytes, which play a pivotal role in hyper humoral immunity, were increased relative to control B-1.

Feed additives according to the present invention increased the antibody titer, it can be seen that significantly increased MHC-class II and CD4 T lymphocytes that play a pivotal role before the immune control.

Test Example 3

Two groups of 40 pigs of 30-day-old pigs were fed the feed additive prepared in Example 2 together with the general formula feed, and test group A-2 (n = 20). Porcine cholera vaccination was not performed separately during the feeding period before 9 weeks of age with B-2 (n = 20). Pigs were challenged with 2 ml of HCV reading 10 ⁴ TCID ₅₀ at the time of 9 weeks of age of pigs in each test group. Immune cell changes were measured before and after swine cholera challenge for each test group A-2 and control group B-2 during the feeding period of up to 14 weeks of age.

Investigation of the effects of leukocyte surface monoclonal antibody, major histocompatibility complex and lymphocyte subpopulation distribution in blood on the white blood cell surface monoclonal antibody isolated in the same manner as in Example 2 for each of test group A-2 and control group B-2 It was. The test results are as follows.

Piglet Cholera Immunity Enhancement Test in Pig division Vaccine Antibody Titer ¹ Immune Cell Activity ² MHC-Class II B Cell Test group A-2 (n = 20) ⁴ after processing 3,712.0 19.04 ± 0.86 16.14 ± 2.15 ³ before processing 7.1 NT NT Control group B-2 (n = 20) After treatment 1,312.0 12.09 ± 1.23 11.90 ± 1.15 Before treatment <4 14.64 ± 1.79 10.81 ± 1.50

1,2,4: 20 days after vaccination without vaccine and virus

3: immediately after implementation

※ n is the number of individuals

As shown in the test results, the increase in vaccine antibody titer was 3,712 in test group A-2, which was significantly higher than 1,312 in control group B-2, and MHC-class II in the case of MHC-class II in the activity of immune cells. 2 increased relatively higher than control B-2, and in the case of B Cell, test group A-2 increased relatively higher than control B-2.

The feed additive according to the present invention increased the antibody titer and increased the MHC-class II, which plays a pivotal role, in part, in the immune control mechanism.

As a result of the test examples 2 and 3, the feed additive in the present invention increased antibody titers and increased B cells, including MHC-class II, which plays a pivotal role in part in the immune control mechanism. In other words, it has been shown to increase immune cells involved in cellular and humoral immunity caused by bacteria and viruses.

Test Example 4

In order to determine the fatty acid component in the meat of pork, the feed according to the present invention was analyzed and compared with the meat component of the test group fed the feed additive according to the present invention and the control group fed the general compound feed under the same conditions. .

Control group A-3 (n = 15), which is fed the feed additive of the present invention, and control group B-3, which is supplied under the conditions of commercially available general blended feed, for two groups of 30 pigs of 30-day-old pigs fed in Test Example 1 ( n = 15) was analyzed until meat before shipment.

Comparison of Pork Ingredients ingredient Test group A-3 (n = 15) Control group B-3 (n = 15) Saturated fatty acid (%) 34 41 Unsaturated fatty acid (%) 66 59

As a result of this experiment, pork fed the feed additive according to the present invention showed about 7% lower in saturated fatty acid content, which is the main cause of adult disease than the control group. Appeared. This means that the test group fed the feed additive according to the present invention is more suitable for preventing adult diseases.

As shown in the above test example, feeding the feed additive of the present invention to livestock is fast and excellent in immunity and grows healthy, so it does not need any emergency measures according to the disease, and the quality of the beef is very soft and excellent. Compared to general pork, it has an excellent effect on producing high protein, low cholesterol, low fat and high quality pork.

Claims (2)

A feed additive comprising 0.05 to 0.1 parts by weight of bamboo vinegar solution, 0.1. To 0.3 parts by weight of bamboo charcoal powder, 0.01 to 0.03 parts by weight of bamboo salt, and 0.06 to 0.12 parts by weight of boiled rice. The method of claim 1, wherein when feeding the feed additives to livestock, 500g to 1,000g of bamboo vinegar solution, 1,000g to 2,000g, charcoal salt 100g to 300g, and 600g of sulfur on the basis of 1,000kg (1 ton) of general compound feed A feed additive, characterized by adding a feed additive composed in the range of ˜1,200 g.