KR20030092515A - Composition comprising Aneilema keisak extract for preventing and curing wart and other epidermis proliferating disease such as psoriasis, ichthyosis - Google Patents

Abstract

PURPOSE: Provided is a composition comprising an Aneilema keisak extract for preventing and treating wart and other epidermis proliferating disease such as psoriasis and ichthyosis. The composition is applied to cosmetics or pharmaceutical preparations. CONSTITUTION: A composition is characterized by comprising an Aneilema keisak extract for preventing and treating wart and other epidermis proliferating disease selected from the group consisting of psoriasis and ichthyosis. It contains the extract in the amount of 0.001-20%, based on the total weight of the composition.

Description

Composition comprising Aneilema keisak extract for preventing and curing wart and other epidermis proliferating disease such as psoriasis, ichthyosis}

The present invention is a wart treatment or other skin epidermal proliferation including psoriasis and juvenile, including wart grass extract containing a component that inhibits the growth of warts caused by papilloma virus and decomposes the desmosome of skin tissue It relates to a cosmetic or pharmaceutical composition for the treatment and prevention of diseases by.

In general, warts are caused by the human papilloma virus (HPV), a DNA virus belonging to the papova virus, which occurs on the back of the hand, around the nail, on the lips, tongue, ear, nose, larynx, and sole of the foot.

Conventional warts can be treated with electrocautery, cryotherapy, acid, Podophyllin, laser, 5-fururauracil, DNCB, and the like. In the case of electrocautery, scars develop and may recur in 20-40%. 25% grapefilin is difficult to use on more than 3 cm in diameter and mucosal areas. CO ₂ lasers have a 90% cure rate. DNCB treatment can treat squamous warts at a 60% therapeutic rate. In addition, there are several treatments such as interferon, bleomycin, retinoids, cantharidine, formalin, and irradiation, but no satisfactory drugs have been developed.

The present invention has been made to solve the above problems, and the object of the present invention is to provide a composition for the treatment and prevention of diseases caused by skin epidermal proliferation, such as warts, psoriasis and mammary glands.

1 is a photograph of the wart grass extract warts tissue. In Figure 1 a is an extract 10%, b is 5%, c is 1%, d is 0.1%, e is incubated for 5 days in a culture containing no extract.

Figure 2 is an electron micrograph of the wart grass extract specifically breaks down the adhesive plaques of the wart tissue so that cells are separated one by one. In Figure 2 ad is a low magnification (3,000 times), eh is a high magnification (e, h is 20,000 times, f, g is 12,000 times) is a photograph taken, a, e is an extract 10%, b, f is 5% , c, g is 1%, d, h is incubated for 5 days in a culture containing no extract.

In order to achieve the above object, the present invention provides a cosmetic or pharmaceutical composition that is effective in the treatment and prevention of diseases caused by skin epidermal proliferation, such as warts and psoriasis comprising an effective amount of mole extract as an active ingredient.

In the present invention, the mole extract may be concentrated, and the mole extract may irritate the skin at 10% or more of the 100-fold concentrate, and may cause problems at low concentrations of 0.001% or less. It is preferable to apply the extract to the skin containing 0.001% -20% or 0.2% -10% 100-fold concentrate.

Praying Mantis grass ( Aneilema keisak (Hasskarl) Hand.-Mazz) is a perennial herb of the monocotyledonous botanical chickweed, growing in marshes and 10-30 cm in size. As the base of the stem extends to the side, the branches split and a beard root comes out from each node. It is reddish purple and has a hair on the stem. The leaves are displaced and the tips are gradually pointed and shiny. When dried out herbs are used as herbal medicines, they are called Murdannia triguetra (Wall.) Bruckn.

As the mantis grass extract used in the present invention, the mantis grass is ground to 10-200 mesh size and extracted by adding at least one solvent selected from an extraction solvent, preferably purified water, ethanol, methanol, butanol, ethyl acetate and acetone. It is used as an extract.

The composition of the present invention can be formulated and used in soap cream ointment, etc. Which formulation is used is a matter that can be determined very easily by those skilled in the art. In addition, the mole extract of the present invention can be conveniently added to basic cosmetics, such as lotions and essences, and can be used for the treatment and prevention of warts and other diseases caused by epidermal hyperplasia.

In addition to the extract of the present invention, the cosmetic composition includes purified water, monohydric or polyhydric alcohols, fatty acid oils, surfactants, and the like, and other flavoring agents, coloring agents, and preservatives.

In the solubilized state, there are purified water, surfactants, monohydric or polyhydric alcohols, and the like, flavoring agents, coloring agents, and preservatives are used as in the emulsion phase.

The present invention can be prepared in any form by conventional manufacturing methods according to the respective needs, such as liquid, cream, paste and solid phase.

Hereinafter, the present invention will be described in more detail with reference to non-limiting examples.

Example 1: Mantis grass extraction method

After 100 grams of mantis grass were washed well with water, the water was removed, the weight of the mantis grass and the same amount of water were added, and then ground with a grinder (Hanil food mixer). The ground mantis pulverized liquid was centrifuged at 250 xg for 10 minutes in a centrifuge. The supernatant was collected and filtered with a 0.22 um pore size filter to remove impurities and used as an additive to the culture.

Example 2: Wart Tissue Degradation Experiment of Mantis Grass Extract

The culture medium for tissue culture was prepared by sterilizing the RPMI 1640 medium with a filter and adding 10% bovine serum and 1% antibiotics. Then, the wart tissue was thinly cut and soaked in 70% alcohol for 1 minute to remove general bacteria. The culture solution was prepared to include 10%, 5%, and 1% wart grass extract in the culture solution, and the wart tissues were immersed therein and put into a 37 ° C. 5% CO ₂ incubator for 5 days. After 5 days, the culture vessel was taken out, and the change in tissue shape was observed and photographed. Warts incubated for 5 days in a solution containing no wart extract retained their tissues, while the warts were cultured into small pieces and the concentration of the mole extract was high. The smaller the pieces, the more they were broken down into powder.

Example 3: Electron micrographs of wart tissues degraded by wart grass extract

The tissues reacted in Example 2 were fixed in Karnovskys fixative (2% paraformaldehyde, 2% glutaraldehyde, 0.5% calcium cloride, pH 7.2) in the cacodylate buffer and fixed at room temperature for 2 hours. After washing twice with Cacodylate buffer, it was soaked in 50%, 60%, 70%, 80%, 90%, 95% 100% alcohol solution for 5 minutes and then planted in the epon mixture. Reaction was carried out at 37 ° C. for 6 hours, 48 ° C. for 12 hours, and 60 ° C. for 24 hours to form a block. The block was cut to 50 nm thickness with an ultrathin cutting machine (Reichert Jung Ultracut S, Leica, Vienna, Austria) and photographed while observing with a microscope (Zeiss EM 902 A electron microscope, Leo, Oberkohen, Germany). The wart tissue that was firmly attached was confirmed that the cells were dropped one by one as the adhesion plate was broken down. The wart tissues cultured for 5 days in a solution containing no wart grass extract still have a firm adherence between the cells, and even when the cells fall off, the adherents that hold the cells together remain intact. It appeared to be falling apart. On the other hand, wart tissues cultured for 5 days in a solution containing wart herb extract are dissolved and dissipated by the specific melting of the adherents that bind cells to cells. Disintegration was observed. The higher the concentration of the mantis grass extract, the more the tissues disintegrated.

Example 4 Preparation of Cosmetic or Pharmaceutical Compositions Comprising Mantis Grass Extract

Cosmetic preparation example: 1) Alcohol and water cosmetic skin:

Ethanol 20.00-70.00 wt%,

Salicylic Acid 0.10-0.50

Triclosan0.05-0.30

Combination fragrances

Mantis Grass Extract 0.01-10.00

Allantoin0.01-0.20

Dipotassium glycyrginate 0.01-0.10

PC-Zinc0.01-0.10

1,3-butylene glycol 1.00-10.00

Take purified water and make 100%.

2) Alcohol and Water Aqueous Gels

Ethanol 20.00-45.00 wt%,

Salicylic Acid 0.10-0.50

Triclosan0.05-0.30

Campa0.01-0.05

Combination fragrances

Carboxy vinyl polymer 0.03-1.20

Triethanolamine 1.00-2.50

Mantis Grass Extract 0.01-10.00

Allantoin0.01-0.20

Dipotassium glycyrginate 0.01-0.10

PC-Zinc0.01-0.0.05

1,3-butylene glycol 1.00-10.00

Take purified water and make 100%.

Examples of pharmaceutical compositions:

Praying Mantis Extract0.01-20 wt%

Preservative 0.05-0.5

hydrophilic ointment base 79.50-99.94

The wart grass extract of the present invention having the configuration as described above may be used as a therapeutic agent for warts, corns, and psoriasis and young glands, which proliferate in the epidermis by promoting decomposition of wart tissues.

Claims (2)

A composition for the treatment and prevention of diseases caused by epidermal proliferation of one skin selected from the group consisting of warts, psoriasis and young glands comprising an effective amount of the mole extract. The method of claim 1, The mole extract is a composition, characterized in that 0.001% to 20% by weight of the composition as a whole.