JP2005232163A - Depilation accelerating agent, depilation accelerating composition, and depilation accelerating method - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To obtain a depilation accelerating agent having excellent depilation accelerating action, lowly dangerous for a human body, and having an excellent skin moisturizing effect, to obtain a depilation accelerating composition, and to provide a depilation accelerating method. <P>SOLUTION: The depilation accelerating agent contains an extract of seaweed belonging to the Genus Eucheuma of Family Solieriaceae as an active ingredient, so that the agent has the excellent depilation accelerating action, is lowly dangerous for the human body, and has the excellent skin moisturizing effect. The depilation accelerating composition having excellent quality is composed by using the depilation accelerating agent. Further, the depilation accelerating method having high depilation accelerating efficiency without accompanied with pains is provided. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to a hair loss promoter containing an extract of seaweed belonging to the genus Eucheuma as an active ingredient, a hair loss promoting composition using the same, and a hair loss promoting method.

  In general, the hair on the head is desired to be rich, while the body hair on the hands, feet, wrinkles, face, etc., tends to be undesirably undesirably aesthetic in appearance.

  Examples of methods for removing such unwanted hair include a hair removal method using a shaver and a tweezer, and a physical hair removal method by applying wax or attaching / peeling a tape. In addition to being uncomfortable, there are problems such as rough skin caused by placing a burden on the skin. In addition, waste hair will regenerate and become conspicuous in a few days. Therefore, it is desired to develop a care that can remove hair without causing pain or discomfort and that maintains the hair removal effect.

  On the other hand, a cathepsin D production promoter (for example, Patent Document 1) and a herbal medicine extract (for example, Patent Document 2) are used as a hair removal method that suppresses hair growth without causing pain or discomfort and maintains the hair removal effect. Hair growth inhibitors that have been reported have been reported.

JP 2003-95903 A JP 2003-171249 A JP 2001-131049 A

  However, there is still no sufficient hair removal method with little pain and discomfort, and it is desired to provide a hair removal promoter that does not burden the skin. In addition, the hair growth inhibitory effect of the hair growth inhibitor is not yet sufficient, and it is desired to provide a hair growth inhibitor that has a higher hair growth inhibitory effect and is less irritating to the skin.

  The present invention has been made in view of the above, and has an excellent hair loss promoting action and hair growth suppressing effect, and provides a hair loss promoting agent that is less irritating to the skin and less painful and uncomfortable. With the goal.

  As a result of intensive studies to achieve the above-mentioned object, the present inventors have found that the extract of seaweed belonging to the genus Milinaceae (Eucheuma) has an excellent hair loss promoting effect and hair growth inhibiting effect, The present invention has been accomplished.

  In addition, the inventors of the present invention have confirmed the safety of the extract of seaweed belonging to the genus Eucheuma, and no problems are found in items such as acute toxicity, skin irritation, skin sensitization, Confirmed that the danger to the human body is low.

  That is, the hair loss promoter of the present invention is characterized by containing an extract of seaweed belonging to the genus Myrhinaceae as an active ingredient. The hair removal promoting composition of the present invention is a composition containing the hair removal promoting agent of the present invention, and promotes hair removal at the desired site by applying it to the desired site such as hands, feet, heels, and face. It is a composition that makes body hair thin, makes it difficult to grow, and makes unwanted hair inconspicuous. Moreover, the method for promoting hair loss of the present invention is a method for promoting hair loss using an extract of a seaweed belonging to the genus Myrhinaceae.

  The “hair removal promoter” as used in the present invention has an action of promoting hair removal, and is added to the target composition to prepare a hair removal promoting composition that has the effect of making body hair inconspicuous. Means a drug. By applying the hair removal promoting composition containing the hair removal promoting agent of the present invention to the hair growth sites of the hands, feet, wrinkles, face, etc., the effect of hair removal can be obtained, and the unwanted hair without causing pain Can be processed. Furthermore, the hair removal promoter of the present invention also has an effect of making it difficult for new hair to grow, and can maintain the hair removal effect.

  Further, the “extract” in the “extract of seaweed belonging to the genus Myrhinaceae” referred to in the present invention means a component having an effect of promoting hair loss, which is obtained by extraction treatment of seaweed belonging to the genus Myrhinaceae. In addition to the extract obtained by extracting seaweeds belonging to the genus Myrhinaceae and the dry solids thereof, the crude product obtained by purifying the extract by a fractionation method, etc. Is also included.

  Conventionally, seaweed extracts belonging to the genus Eucheuma have been known to have an effect of improving moisture retention on the skin (for example, Patent Document 3). However, it is not known at all that the extract of the seaweed belonging to the genus Eucheuma belonging to the family Milinaceae has a hair loss promoting action, and it is a new finding by the present inventors that the extract promotes hair loss. It is. Moreover, it is a new finding by the present inventors that the above extract suppresses hair growth of body hair.

  The hair removal promoter of the present invention exhibits an excellent hair removal promoting effect and can be suitably used for hair removal, hair removal or shaving-related cosmetics. Moreover, the hair loss promoter of the present invention also has an effect of suppressing hair growth.

  Moreover, the active ingredient of the hair loss promoter of this invention is extracted from the seaweed used for food etc. from old times. Therefore, since the safety to the human body is extremely high and the moisturizing effect is high, it is possible to provide a safe hair removal promoter with low irritation to the skin.

Hereinafter, embodiments of the present invention will be described.
The hair loss promoter of the present invention is characterized by containing an extract of seaweed belonging to the genus Myrhinaceae as an active ingredient.

The seaweeds belonging to the genus Eucheuma used in the present invention include the following: Eucheuma cottonii) and the like are used, and most preferred are Eucheuma spinosum and Eucheuma cottonii. In addition, for example, giraffe rhinoceros (Euchuma muricatum) has a cylindrical columnar trunk, irregular branches in each direction, and the branches are entangled with each other to form a considerably large lump. It is an agar and paste algae and is distributed in the Nansei Islands and Australia.

  A method for obtaining an extract of seaweeds belonging to the genus Myrhinaceae is not particularly limited, and a normal extraction method is adopted, using water, a hydrophilic organic solvent, a hydrous hydrophilic organic solvent, other organic solvents, and the like. Extracted from seaweed. Examples of the hydrophilic solvent include lower alcohols such as methanol, ethanol and isopropanol, acetone, methyl ethyl ketone, acetonitrile, dimethyl sulfoxide, dimethylformamide and the like.

  Among these, it is particularly preferable to use water or a mixed solution of water and a lower alcohol such as methanol, ethanol, or isopropanol as the extraction solvent. In this case, the ratio of water and lower alcohol is preferably 100/0 to 30/70 (V / V, volume ratio) of water / lower alcohol. The ratio of the seaweed bulk to the extraction solvent is preferably such that the seaweed bulk (dry matter) / solvent ratio is 1/50 to 1/2 (W / W, mass ratio).

  Although there is no restriction | limiting in particular in extraction temperature, Preferably it is 5 to 80 degreeC, and it is preferable to perform extraction processing, stirring for 1 to 24 hours. The extraction pH is not particularly limited as long as it does not tend to be extremely acidic or alkaline.

  This extract can be used as it is as an extract of a seaweed belonging to the genus Myrhinaceae. Preferably, a high molecular weight fraction is prepared from this extract, and the high molecular weight fraction (hereinafter referred to as “high molecular weight fraction”) is used. It is preferable to use "molecular weight extract". Specifically, the molecular weight of the high molecular weight extract is preferably 100,000 or more. There is no restriction | limiting in particular as a fractionation method, A normal fractionation method is employ | adopted. Specific examples include a fractionation method using an ultrafiltration membrane filter having a fractional molecular weight of 100,000.

  This extract or the high molecular weight extract fractionated from this extract can be used as it is as a hair loss promoter, or it can be used as a diluted solution or a concentrated extract. Moreover, you may use as a hair removal promoter what was made into the dry powder by freeze-drying etc., or was prepared in the paste form. When it is prepared as a dry powder, it is preferably used by dissolving it in water or a lower alcohol such as methanol, ethanol or isopropanol containing water in advance, or solubilizing it in an external preparation containing water described below.

  In addition, by adding the hair removal promoter of the present invention to a predetermined target composition, the target composition can be given an effect of removing hair and maintaining the hair removal effect.

  By adding the hair loss promoter of the present invention to a predetermined target composition, the target composition can be imparted with an effect of making it difficult for hair to grow and making unwanted hair inconspicuous.

  When the hair loss promoter of the present invention is added to a predetermined target composition, the amount of seaweed extract belonging to the genus Myrhinaceae in the target composition varies depending on the product form and frequency of use, and is generally defined. However, the lower limit concentration is 0.01% by mass or more, preferably 0. 0%, based on the dry solid weight of a fraction having a molecular weight of 100,000 or more obtained from an extract of seaweed belonging to the genus Myrhinaceae. 1 mass% or more, More preferably, it is 1 mass% or more. On the other hand, the upper limit concentration is 50% by mass or less, preferably 30% by mass or less, more preferably 10% by mass or less, and particularly preferably 5% by mass or less. In particular, in the range of 1 to 5% by mass, the effect of promoting hair loss tends to be recognized depending on the concentration.

  As described above, since the hair loss promoter of the present invention has a low risk to the human body, it is particularly suitable for products that are applied to the skin, in particular, to sites where unwanted hair grows such as hands, feet, wrinkles, and faces. Can be included. The product to which the hair removal promoter of the present invention is applied is not particularly limited, but is preferably a hair removal, hair removal or shaving-related product, or animal hair removal or hair removal product. Specifically, hair removal agents such as pastes, creams and aerosols, hair removal agents such as waxes, gels and sheets, lotions used for hair removal or post-treatment of hair removal, post treatments such as creams, Deodorant lotions, deodorant powders, deodorant sprays, antiperspirant and deodorant cosmetics such as deodorant sticks, pre-shaving treatments such as pre-shave lotion, shaving creams such as shaving cream, post-shaving treatments such as after-shave lotion, etc. It is done.

  The hair removal promoting composition containing the hair removal promoting agent of the present invention can be appropriately blended with known medicinal ingredients and raw materials used for usual external preparations as needed within a range not impairing the effects of the present invention. For example, antibacterial agents, anti-inflammatory agents, moisturizers, surfactants, oils, alcohols, thickeners, preservatives, antioxidants, chelating agents, pH adjusters, fragrances, pigments, UV absorbers / scatterers, vitamins , Water, etc. can be blended.

  The hair loss promoting composition of the present invention can be used in the form of a uniform solution, lotion, gel or the like according to a conventional method. Moreover, the hair loss promoting composition of the present invention can take the form of an aerosol. In that case, in addition to the above components, lower alcohols such as n-propyl alcohol or isopropyl alcohol; flammable gases such as butane, propane, isobutane, liquefied petroleum gas, dimethyl ether; nitrogen gas, oxygen gas, carbon dioxide gas, suboxide Compressed gas such as nitrogen gas can be contained.

  Hereinafter, the present invention will be described in more detail based on examples. In addition, this invention is not limited to the following Example.

[Preparation Example 1: Production of extract of seaweed seaweed]
100 g of Eucheuma spinosum was immersed in 2 liters of water, and extraction treatment was performed at room temperature for 3 hours while stirring. Then, 20.3 g of Eucheuma spinosum water extract was obtained by concentration and freeze-drying.
Similarly, an aqueous extract of Eucheuma cottonii was obtained.

[Preparation Example 2: Production of a fraction having a molecular weight of 100,000 or more from a seaweed extract of the genus Kirinsai]
5.0 g of the Eucheuma spinosum water extract obtained in Preparation Example 1 was dissolved in 1 liter of water at 4 ° C. This solution was passed through a filter having a pore size of 0.65 μM and then passed through a filter having a pore size of 0.45 μM to obtain a filtrate. This filtrate was fractionated by filtration using an Amicon YM100 filter (Millipore) with a molecular weight of less than 100,000. The solution on the Amicon YM100 filter was freeze-dried to obtain 1.9 g of a high molecular weight extract having a molecular weight of 100,000 or more.
In the same manner, a high molecular weight extract having a molecular weight of 100,000 or more was obtained from the Eucheuma cottonii water extract.

[Preparation Example 3: Production of an extract having a molecular weight of 50,000 or more and less than 100,000 from an extract of the seaweed of the genus Kirinsai]
In Preparation Example 2 above, the filtrate having a molecular weight of less than 100,000, which was fractionated by filtration using an Amicon YM100 filter (manufactured by Millipore), was filtered using Amicon YM50 (manufactured by Millipore) to obtain a molecular weight of 50,000 or more. It was divided into a fraction and a fraction less than 50000. Further, the above fraction less than 50,000 was divided into a fraction having a molecular weight of 10,000 or more and a fraction having a molecular weight of less than 10,000 using Amicon YM10 (manufactured by Millipore). Each fraction obtained by the above method is freeze-dried to obtain 1.1 g of an extract having a molecular weight of 50,000 or more and less than 100,000, 0.8 g of an extract having a molecular weight of 50,000 or more and less than 50,000, and 0.0. 7 g was obtained.

[Example 1: Mouse application test]
An Eucheuma spinosum extract having a molecular weight of 100,000 or more prepared under the conditions of Preparation Example 2 was dissolved in a solvent (30% ethanol) to a concentration of 5% by mass to obtain a sample.
In the experiment, six 49-day-old C3H / HeNCrj mice having a hair cycle in a resting period were used. A hair growth site of 1.2 cm square on the left and right sides of each mouse on the back of each mouse was used as a test site, the sample was applied to one side, and a solvent was applied to the other side to serve as a control. The application amount was 50 μl for both, and application was performed once a day for 10 days. The effect of the Eucheuma spinosum extract was compared with the application site and the control, scored as follows, and the results are shown in Table 1.

<Score division>
Same as control hair growth: 0 rating
Slight hair removal than control: Rating 0.5
Clearer than control: Grade 1
Hair loss significantly more than control: Grade 2

  From the results shown in Table 1, hair loss was significantly induced in the site where the sample was applied as compared with the control group, and the Eucheuma spinosum extract showed an excellent hair loss promoting effect. Furthermore, it was confirmed that the hair removal effect persisted at the site where the hair removal effect was observed, and that the Eucheuma spinosum extract had an excellent hair growth inhibiting effect.

[Example 2: Mouse application test (difference in effect due to concentration difference)]
An Eucheuma spinosum extract having a molecular weight of 100,000 or more prepared under the conditions of Preparation Example 2 was dissolved in a solvent (20% ethanol) to a concentration of 1% by mass, 3% by mass, and 5% by mass to obtain a sample.
In the experiment, 12 53-day-old C3H / HeNCrj mice having a hair cycle in the resting period were used (three individuals were used in each group of control, 1% by mass, 3% by mass, and 5% by mass). In order to accurately identify the test site, the hair on the entire back of the mouse was shortened with a clipper. Only 100 μl of either sample or solvent was applied to the back test site of each mouse once a day for 10 days. The effect of application of the Eucheuma spinosum extract (sample) was compared with the control group to which only the solvent was applied, and scored as follows. The results are shown in Table 2.

<Score division>
Same as control group hair growth: 0 rating
Slightly less hair than the control group: Rating 0.5
Clearer hair loss than control group: Grade 1
Hair loss markedly higher than the control group: Rating 2

  From the results in Table 2, the hair removal promoting action of the Eucheuma spinosum extract was recognized in a concentration-dependent manner within a concentration range of 1 to 5% by mass. When the concentration was 5% by mass or more, it was insoluble in the solvent (20% ethanol) used in this example.

[Example 3: Mouse application test (difference in effect due to difference in molecular weight)]
Extract of Eucheuma spinosum prepared in Preparation Example 2 (extract with a molecular weight of 100,000 or more) and extract of Eucheuma spinosum prepared in Preparation Example 3 (extract with a molecular weight of 50,000 to less than 100,000, extract of 10,000 to less than 50000, 10000 Less than extract) was dissolved in a solvent (20% ethanol) so as to have a concentration of 3% by mass, respectively, to prepare a sample.
In the experiment, 15 53-day-old C3H / HeNCrj mice (three in each of the control and sample groups) having a hair cycle in the resting period were used. In order to accurately identify the test site, the hair on the entire back of the mouse was shortened with a clipper. Only 100 μl of either sample or solvent was applied to the back test site of each mouse once a day for 10 days. The effect of application of each extract (sample) was compared with the control group to which only the solvent was applied, and scored as follows. The results are shown in Table 3.

<Score division>
Same as control group hair growth: 0 rating
Slightly less hair than the control group: Rating 0.5
Clearer hair loss than control group: Grade 1
Hair loss markedly higher than the control group: Rating 2

  From the results of Table 3, it was confirmed that among the Eucheuma spinosum extracts, a high molecular weight extract having a molecular weight of 100,000 or more has a hair loss promoting effect.

[Example 4: Product formulation example]
Hereinafter, the formulation example (hair removal promotion composition) which mix | blended the hair removal promoter of this invention is shown. In addition, in each table | surface, "the extract of Eucheuma spinosum" and "the extract of Eucheuma cottonni" represent the high molecular weight extract of molecular weight 100000 or more prepared on the conditions of the said preparation example 2.

(Prescription Example 1 Hair Removal Lotion)
A hair removal lotion was prepared according to the blending ratio shown in Table 4 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製
「スタノロン」：５α−Ａｎｄｒｏｓｔａｎ−１７β−ｏｌ−３−ｏｎｅ、シグマアルドリッチジャパン株式会社製

“Bromelain F”: protease preparation, manufactured by Amano Enzyme Co., Ltd. “Stanolone”: 5α-Androstan-17β-ol-3-one, manufactured by Sigma Aldrich Japan Co., Ltd.

  When this hair removal lotion was used to conduct a mouse application test in the same manner as in Example 1, it was found that the hair removal promoting effect was excellent and the safety was also good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

(Prescription Example 2 Hair Removal Cream)
A hair removal cream was prepared according to the blending ratio shown in Table 5 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製

“Bromelain F”: Protease preparation, Amano Enzyme, Inc.

  Using this hair removal cream, a mouse application test was conducted in the same manner as in Example 1. As a result, it was found that the hair removal promoting effect was excellent and the safety was good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

(Prescription Example 3 Hair Removal Cream)
A hair removal cream was prepared according to the blending ratio shown in Table 6 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製

“Bromelain F”: Protease preparation, Amano Enzyme, Inc.

  Using this hair removal cream, a mouse application test was conducted in the same manner as in Example 1. As a result, it was found that the hair removal promoting effect was excellent and the safety was good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

(Prescription Example 4 Hair Removal Gel)
A hair removal gel was prepared according to the blending ratio shown in Table 7 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製

“Bromelain F”: Protease preparation, Amano Enzyme, Inc.

  Using this hair removal gel, a mouse application test was conducted in the same manner as in Example 1. As a result, it was found that the hair removal promotion effect was excellent and the safety was good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

(Prescription Example 5 Hair Removal Foam)
A depilatory foam was prepared according to the blending ratio shown in Table 8 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製

“Bromelain F”: Protease preparation, Amano Enzyme, Inc.

  When this hair removal foam was used and a mouse application test was conducted in the same manner as in Example 1, it was found that the hair removal promoting effect was excellent and the safety was also good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

(Prescription Example 6 Hair Removal Spray)
A hair removal spray was prepared according to the blending ratio shown in Table 9 below.

「ブロメラインＦ」：プロテアーゼ製剤、天野エンザイム株式会社製

“Bromelain F”: Protease preparation, Amano Enzyme, Inc.

  Using this hair removal spray, a mouse application test was carried out in the same manner as in Example 1. As a result, it was found that the hair removal promoting effect was excellent and the safety was good. Furthermore, it was also confirmed that the hair removal effect persisted at the site where the hair removal effect was observed and that there was an excellent hair growth inhibiting effect.

  As described above, the hair loss promoter according to the present invention has an excellent hair loss promoting effect and has a low risk to the human body, so that it is applied to the skin, in particular, to the site where unwanted hair grows such as hands, feet, wrinkles and face. It can be used for products to be applied, and is particularly suitable for hair removal, hair removal or shaving-related products, and animal hair removal, hair removal-related products.

Claims (5)

  A hair loss-promoting agent comprising an extract of seaweed belonging to the genus Milinaceae as an active ingredient.   The molecular weight of the said extract is 100,000 or more, The hair loss promoter of Claim 1 characterized by the above-mentioned.   A hair loss promoting composition comprising the hair loss promoting agent according to claim 1 or 2.   A method for promoting hair loss, characterized by promoting hair loss using an extract of a seaweed belonging to the genus Myrhinaceae.   The method for promoting hair loss according to claim 4, wherein a molecular weight of the extract is 100,000 or more.