KR20030079185A - Processing method and its Application of Medicine for Treating Mastitis of Expectant and Dairycow Using Dandelion Extract - Google Patents

Abstract

PURPOSE: Provided is a method for manufacturing a therapeutic agent for mastitis in pregnant women and nursing mothers as well as milch cows using an extract of Taraxacum officinale Web. The extract of Taraxacum officinale Web. has high antibacterial activity. CONSTITUTION: A method for manufacturing a therapeutic agent for mastitis in pregnant women and nursing mothers is characterized b the steps of; dipping Taraxacum officinale Web. in boiling water at 95-100 deg.C for 7 hours and followed by filtration; re-extracting the Taraxacum officinale Web. by dipping it in 25-30% ammonia solution for 7-10 days, and followed by separation of tinture from the extract; and mixing the filtrate and the tinture to pH 10.

Description

Processing method and its Application of Medicine for Treating Mastitis of Expectant and Dairycow Using Dandelion Extract}

The present invention relates more specifically to veterinary medicine and more specifically to methods of producing new pharmaceuticals for the treatment of mastitis in animals and humans, which are used not only for the medical practice of treating mastitis in humans but also for the veterinary treatment of various mastitis in all kinds of animals. do.

The present invention is based on the provision of a method for producing a novel pharmaceutical which is very effective in treating mastitis, is non-toxic and has no side effects.

Methods of producing various chemical pharmaceuticals for the treatment of mastitis in animals and humans are known techniques. Most of these drugs are the most commonly used antibiotics, with penicillin and streptomycin (81%).

Methods of preparing various compositions in which these antibiotics are combined with oil bases, peredinisols, foaming agents and accelerators in the treatment of mastitis in animals are well known. These compositions are prepared by mixing in the starting element. However, the availability of the compositions is not sufficient. Antibiotic drugs are used to treat mastitis, which can cause side effects and lower gastrointestinal function. The present invention is an invention for treating mastitis or mastitis of pregnant women and cows by extracting and purifying extracts with high antibacterial activity from pogongyoung belonging to the Asteraceae.

In general, pogongyoung is an outpost of asteraceae, its main components are Taraxacin (Taraxacin), Taraxalol (Taraxarol), Taraxaccoside, various fatty acids, Inulin, β-Sitosterol, Β-Amyrin and the like are known.

The use of pogongyoung as a medicinal herb is known to have medicinal pharmacological effects such as synergism, medicinal metabolism, etc., including antipyretic detoxification, edema, diuresis, acute mastitis, conjunctivitis, bronchitis, antifungal effect, palliative action, and antibacterial effect.

In addition, Pogongyoung is used as a diuretic by separating and purifying water-soluble extracts in modern medicine, and since it is not toxic to humans, it is powdered and then sold as health food.

On the other hand, no research on the antimicrobial activity of pogongyoung has been published yet, and the main studies to date have been made on the components thereof, by Triterpene compounds by R. Aexel and S. Evans, H. Nitsche, etc. Nexaanthin, Taraxacoside by Hans-WilliRauwald and the like.

Therefore, the present inventors not only used an inexpensive solvent such as water, ethanol, ethyl acetate, etc. in order to increase the industrial economics in extracting and purifying the extract having high antimicrobial activity from the herbal medicine poongongyoung, only organic solvent during the purification process We have developed a very efficient and simple method to use. The antimicrobial activity was verified through in vitro experiments of the extracts obtained in this way, and the acute toxicity test proved that the extract was not human toxic.

Accordingly, an object of the present invention is to provide a simple and economical extraction and purification method using only an inexpensive organic solvent in extracting and purifying antimicrobial active substances which are not toxic to human body from poongongyoung.

First, in extracting an excellent antimicrobial activity from Poongyoung,

Second, in the treatment of mastitis or mastitis of dairy cows and pregnant women using the extract,

Third, by improving the extraction method of Pogongyoung, maximize yield, reduce production cost and establish mass production system.

Fourth, to provide an applicability using pogongyoung extract.

Dandelion (Taraxacum officinale Web.) For the treatment of human and animal mastitis is soaked in boiling water at 95-100 ° C for at least 7 hours, and then the extract is filtered and immersed in 25-30% aqueous ammonia solution for 7 to 10 days. After baking, the extract and the tincture of the same weight (tinture) is separated and the above-mentioned herbal extract and tincture (tinture) to a pH of at least 10, characterized in that the method for producing a drug for the treatment of human and animal mastitis.

In the method of preparing tincture, 1 kg of crushed pogongyoung dry powder is placed in an extractor, and 3-5 L of ethanol, which is a nonpolar solvent, is added thereto, stirred at 60 ° C for 6 hours, and filtered to obtain a pogongong residue. This residue is added to an extractor, 3 to 5 L of 70-100% lower alcohol is added, followed by stirring and extraction at 50 ° C for 6 hours. After extraction, it is filtered and inorganic salts are added to the filtrate to adjust the pH to neutral and then concentrated.

To this, 2 to 3 L of chloroform and 2 to 3 L of distilled water are added to obtain a water-soluble extract liquid separated in layers.

The resulting aqueous extract was concentrated under reduced pressure to 1 to 1.5 L at 60 ° C to remove the organic solvent. 0.1-0.2N inorganic acid is added to it, and pH concentration is adjusted to 3-5.

At this time, if the pH concentration is less than 3, there is a problem in that the active ingredient is deteriorated. If the pH is more than 5, a part of the active ingredient may be precipitated between layers when the organic solvent is separated from the organic solvent.

Ethanol is added 1 to 1.5 liters three to four times, and the layers are separated. The organic solvent layer is collected, and concentrated under reduced pressure at 60 ° C. or lower to obtain 6-8 g of extract.

10-20% of a lower alcohol aqueous solution and 100-200 ml are added to the obtained extract and dissolved, and calcium carbonate is added to adjust the pH to 5-7.

At this time, if the PH concentration is less than 5, there is a problem in that the active ingredients are diffused into each layer when the layer is separated from the organic solvent, and if it exceeds 7, some of the active ingredients may be precipitated between the layers when the layer is separated from the organic solvent.

100-200 ml of a halogen compound or lower ketone is added thereto, and the layers are separated. The aqueous layer is concentrated under reduced pressure at 60 ° C. or lower to obtain 3.5-4.5 g of pogongyoung extract having antimicrobial activity.

In the above process, when the% content of the lower alcohol is less than 10%, the active ingredient is precipitated between the aqueous layer and the organic solvent layer, and when more than 20%, the active ingredient is diffused in both the aqueous layer and the organic solvent layer. Since the purified extract is very low in human toxicity, it is suitable for external skin preparations as well as oral preparations.

Hereinafter, the present invention will be described in detail with reference to the following examples, and the present invention is not limited by the examples.

Example 1

Dandelion (Taraxacum officinale Web.) For the treatment of human and animal mastitis is soaked in boiling water at 95-100 ° C for at least 7 hours, and then the extract is filtered and immersed in 25-30% aqueous ammonia solution for 7 to 10 days. After baking, the extract and the tincture of the same weight (tinture) is separated and the above-mentioned herbal extract and tincture (tinture) to a pH of at least 10, characterized in that the method for producing a drug for the treatment of human and animal mastitis.

Example 2

The antimicrobial effect on the pogongyeong extract obtained in Example 1 is shown in the following Table 1, MIC (Minimum Inhibitory Concentration) value was measured after incubation for 24 hours by the (Broth dilution) method by Ericsson (Ericsson).

Example 3

The agent according to the invention was used for the treatment of 40 cows suffering from mastitis with different degrees of illness.

Type of disease Treatment number Processing period Treatment effect Acute mastitis 15 5 days All were done three or more times a day to complete. Fibrous mastitis 10 4 days Purulent mastitis 5 4 days Chronic mastitis 10 5 days

The medicament according to the invention was milked daily and successively injected into the udder skin of a sick animal after a light massage of 5-7 minutes in succession.

Therefore, after the drug treatment according to the present invention was completely treated to recover milk production.

(Example 4)

Forty-eight animals with chronic mastitis in 80 cows were selected for testing. In clinical trials, sick animals had the following forms of mastitis:

Type of disease Treatment number Processing period Treatment effect Intestinal mastitis 4 10 Cured Intestinal Qatar Mastitis 15 8 Cured Catarrhal mastitis 20 10 Cured Fibrous mastitis 3 9 Cured Purulent mastitis 5 10 Cured Hemorrhagic mastitis 5 11 Cured

(Example 5)

The effect of mastitis on 30 animals was confirmed in 1000 cow farms.

Type of disease Treatment number Processing period Treatment effect Intestinal Qatar Mastitis 7 6 Two of the breasts were massaged for 5-10 minutes prior to cure. Fibrous mastitis 8 7 Antibiotic treatment Purulent Qatar Mastitis 15 7 Antibiotic treatment

(Example 6)

For the purposes of testing, 65 mammary cows were selected from dairy farms. Following clinical trials, mastitis in animals is as follows.

Type of disease Treatment number Processing period Treatment effect Qatar Mastitis 40 15 Two of the breasts were massaged for 5-10 minutes prior to cure. Fibrous mastitis 10 10 Purulent Qatar Mastitis 15 15 Antibiotic treatment

(Example 7)

Somatic reduction effect after oral administration of mastitis-infected cows in dairy farms for

(Unit: 10,000 / ml)

Individual number Somatic cells before feeding Somatic cells after feeding (after 15 days) One 120 27 2 80 15 3 70 13 4 60 20 93 68 3 12 54 7 Average 75.33 (100%) 14.16 (18.7%)

(Example 8)

Staphylococcus aureus, a representative strain that causes mastitis in cows, was used to investigate the antimicrobial activity. (First dose bacteria; 2x105 CFU / ml)

<Antibacterial effect against standard strain Staphylococcus aureus ATCC 29213>

Test Death effect (survival colony number) Poongyoung extract administration time 0 min 30 minutes 2 hours 18 hours 24 hours Comparative example No addition 2 × 10 ⁵ 2 × 10 ⁵ 3 × 10 ⁶ 7 × 10 ⁸ 5 × 10 ⁹ 0.1% Poongyoung Extract 2 × 10 ⁵ 1 × 10 ² 6 0 0 0.01% Poongyoung Extract 2 × 10 ⁵ 6 × 10 ⁴ 8 × 10 ² 12 0 0.001% Poongyoung Extract 2 × 10 ⁵ 2 × 10 ⁵ 6 × 10 ⁴ 4 × 10 ² 80

The present invention has the advantage that it is economically easy to obtain the material to the invention to treat mastitis or mastitis of pregnant women and cows by extracting, purifying the extract with high antimicrobial activity from poongongyoung. In addition, pogongyoung extract according to the present invention has a uniform physical properties, has excellent antimicrobial activity, and low production cost. In addition, the process is very simple, it is possible to produce an economical extract without any environmental pollution in the production process.

Claims (4)

Dandelion (Taraxacum officinale Web.) For the treatment of human and animal mastitis is soaked in boiling water at 95-100 ° C for at least 7 hours, and then the extract is filtered and immersed in 25-30% aqueous ammonia solution for 7 to 10 days. After baking, the extract and the tincture of the same weight (tinture) is separated and the above-mentioned herbal extract and tincture (tinture) to a pH of at least 10, characterized in that the preparation method for the treatment of medicaments for human and animal mastitis . The volume% ratio of the resulting extract and tincture to Poongyoung Extract 70-80% Poongyoung tincture 30-20% Characterized by mixing. The scope of application of the extract using claim 1 and claim 2 to cows mastitis, feed additives, human mastitis. The method of adding the extract quality using the claim 1, 2, 3 is the method of adding the cow to the feed and the direct administration to the milk and the method of attaching the extract to the nipple area