KR20030056418A - Tuberculosis Auto Stainer - Google Patents
Tuberculosis Auto Stainer Download PDFInfo
- Publication number
- KR20030056418A KR20030056418A KR1020010086625A KR20010086625A KR20030056418A KR 20030056418 A KR20030056418 A KR 20030056418A KR 1020010086625 A KR1020010086625 A KR 1020010086625A KR 20010086625 A KR20010086625 A KR 20010086625A KR 20030056418 A KR20030056418 A KR 20030056418A
- Authority
- KR
- South Korea
- Prior art keywords
- chamber
- rotating plate
- glass plate
- support member
- groove
- Prior art date
Links
- 201000008827 tuberculosis Diseases 0.000 title description 2
- 239000011521 glass Substances 0.000 claims abstract description 50
- 244000005700 microbiome Species 0.000 claims abstract description 30
- 238000004043 dyeing Methods 0.000 claims abstract description 26
- 238000010438 heat treatment Methods 0.000 claims abstract description 12
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 239000000243 solution Substances 0.000 claims description 24
- 238000009434 installation Methods 0.000 claims description 20
- 238000002347 injection Methods 0.000 claims description 14
- 239000007924 injection Substances 0.000 claims description 14
- 230000000813 microbial effect Effects 0.000 claims description 6
- 238000004891 communication Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 2
- 230000002906 microbiologic effect Effects 0.000 claims description 2
- 238000010186 staining Methods 0.000 abstract description 10
- 239000012192 staining solution Substances 0.000 abstract 1
- 239000000975 dye Substances 0.000 description 27
- 241000894006 Bacteria Species 0.000 description 10
- 239000007921 spray Substances 0.000 description 5
- 238000007689 inspection Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 2
- 208000003322 Coinfection Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229960000907 methylthioninium chloride Drugs 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 206010024229 Leprosy Diseases 0.000 description 1
- 230000001458 anti-acid effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
Abstract
Description
본 발명은 미생물 자동 염색장치에 관한 것으로, 세균(또는 검사하고자 하는 물질)의 감별을 위한, 유리판으로의 세균 고정, 염색액 분무, 염색작용, 탈색, 세척, 건조 등의 미생물 염색을 자동으로 수행하는 미생물 자동 염색장치에 관한 것이다.The present invention relates to a microorganism automatic staining apparatus, for automatically distinguishing bacteria (or substances to be tested), microbiological staining such as fixing a bacterium to a glass plate, spraying a stain solution, staining, decoloring, washing, and drying are performed automatically. It relates to a microorganism automatic staining apparatus.
일반적으로, 미생물 염색방법은 메틸렌블루, 크리스탈 바이올렛 염기성 푸크신등의 염색액을 많이 사용하고, 단순히 세균의 형태만을 관찰할 때 사용하는 단염색(simple stain)과, 크리스탈 바이올렛, 또는 메틸렌 블루 같은 염색액으로 미생물을 살아있는 채로 염색하는 생체염색(vital stain)과, 세균의 감별시 사용되는 감별염색(different stain)으로 분류되며, 상기 감별염색은 다시, 미생물 분류에 널리 사용되고 있는 그람염색(Gram's stain)과, 결핵균이나 나균과 같은 미생물에 속하는 균들을 관찰할 때 사용하는 항산성염색 및 아포염색, 편모염색, 협막염색 등의 특수염색으로 분류된다.In general, the microbial staining method uses a lot of dyes, such as methylene blue, crystal violet basic fuxin, and a simple stain used to simply observe the form of bacteria, and dyes such as crystal violet or methylene blue It is classified into a vial stain which stains microorganisms alive with a liquid and a differential stain which is used for the differentiation of bacteria. It is classified into special dyes such as anti-acid staining and follicle staining, flagella staining, and capillary staining, which are used to observe bacteria belonging to microorganisms such as tuberculosis bacteria and leprosy.
종래에는 상기와 같이 미생물을 염색하고자 할 경우, 염색하고자 하는 시료를 화염멸균한 유리판 위에 도말하고, 이것을 알콜램프 등의 불꽃에 의해 고정시킨 후, 염색액을 떨어뜨려 염색하며, 염색이 끝나면 염색액을 물로 씻어내고, 이를 다시 건조하였다.Conventionally, in order to dye microorganisms as described above, the sample to be dyed is smeared on a flame sterilized glass plate, and fixed with a flame such as an alcohol lamp, and then stained by dropping the dye solution. Washed with water and dried again.
그러나, 상기와 같은 종래의 방법은 모두 수작업에 의해 진행되므로, 매우 번거롭고, 숙련자에 의해 염색작업이 진행되어야 하며, 많은 염색시간이 소요되고, 대상물(미생물)의 균일한 염색이 곤란하였다.However, all of the conventional methods as described above are performed by hand, which is very cumbersome, requires a dyeing operation by a skilled person, requires a lot of dyeing time, and difficulty in uniform dyeing of an object (microorganism).
또한, 비숙련자에 의해 염색을 할 경우, 검사환경이 불결하게 되고, 이로 인해 세균에 의한 2차 감염문제가 발생되는 문제점이 있었다.In addition, when stained by an unskilled person, the test environment becomes dirty, which causes a problem of secondary infection caused by bacteria.
또한, 유리판에 시료를 고정하기 위하여 외부에서 알콜램프 등의 가열수단을 이용하므로, 항시 별도의 장치를 구비하여야 하며, 시료의 고정을 위해 별도의 작업을 해야 하는 등 여러 가지 문제점이 있었다.In addition, since a heating means such as an alcohol lamp is used to fix the sample to the glass plate, there must be a separate device at all times, and there are various problems such as a separate work for fixing the sample.
본 발명은 상기와 같은 문제점을 고려하여 이루어진 것으로, 그 목적은 미생물을 자동으로 염색하여, 염색작업 시간을 단축하고, 검사환경을 청결하게 하며, 검사의 정확도를 향상시킬 수 있는 미생물 자동 염색장치를 제공하는 것이다.The present invention has been made in consideration of the above problems, the object of the present invention is to automatically dye microorganisms, shorten the dyeing time, clean the inspection environment, and improve the accuracy of inspection microbial automatic dyeing apparatus To provide.
본 발명은 미생물 자동염색장치에 있어서; 상기 미생물 자동염색장치는 몸체 상부내에 챔버가 형성되고, 상기 챔버와 연결되어 배수관이 형성되며, 상기 챔버 상부에 위치하도록 몸체에 상부덮개가 설치되는 지지부재와; 상기 챔버내에 설치되고 미생물이 도말된 유리판 다수개가 장/탈착되는 회전판과; 상기 회전판과 연결축에 의해 연결되고 지지부재의 몸체에 설치되는 작동모터와; 상기 회전판의 하부 일측에 자석이 설치되고, 이를 감지하는 자석센서가 지지부재의 챔버 일측에 각각 설치되는 감지부재와; 상기 챔버와 연통되도록 지지부재 상부에 설치된 상부덮개에 설치되고 내부에 열풍기를 구비하는 가열부재와; 상기 지지부재 일측에 연결 설치되고 회전판에 장착된 유리판으로 염색액을 분무하는 분사부재를 포함하여 구성되어, 유리판에 도말된 미생물을 자동으로 고정, 염색, 탈수, 건조하는 미생물 자동 염색장치를 제공함에 있다.The present invention provides a microorganism automatic dyeing apparatus; The microbial automatic dyeing device includes a support member in which a chamber is formed in an upper portion of the body, a drain pipe is connected to the chamber, and an upper cover is installed on the body so as to be positioned above the chamber; A rotating plate installed in the chamber and having a plurality of glass plates smeared with microorganisms; An operating motor connected to the rotating plate by a connecting shaft and installed on a body of the support member; A sensing member provided at one side of the lower side of the rotating plate, and a magnetic sensor sensing the magnet at one side of the chamber of the supporting member; A heating member installed on an upper cover installed above the support member so as to communicate with the chamber and having a hot air fan therein; It is connected to one side of the support member is configured to include a spray member for spraying the dye solution to the glass plate mounted on the rotating plate, to provide a microbial automatic dyeing device for automatically fixing, dyeing, dehydrating, drying the plated microorganisms on the glass plate have.
도 1 은 본 발명에 따른 구성을 보인 정면 예시도1 is a front view showing a configuration according to the present invention
도 2 는 본 발명에 따른 구성을 보인 평면 예시도Figure 2 is a plan view showing a configuration according to the present invention
도 3 은 본 발명 회전판의 구성을 보인 예시도Figure 3 is an exemplary view showing the configuration of the rotating plate of the present invention
* 도면의 주요부분에 대한 부호의 설명* Explanation of symbols for the main parts of the drawings
(10) : 회전부재 (11) : 회전판(10): rotating member (11): rotating plate
(12) : 작동모터 (13) : 연결축(12): working motor (13): connecting shaft
(20) : 지지부재 (21) : 몸체20: support member 21: body
(22) : 챔버 (23) : 배수관22: chamber 23: drain pipe
(24) : 상부덮개 (30) : 감지부재(24): upper cover (30): sensing member
(31) : 자석 (32) : 자석센서(31): magnet (32): magnet sensor
(40) : 가열부재 (41) : 열풍기40: heating member 41: hot air fan
(50) : 분사부재 (51) : 약품통(50): injection member (51): medicine container
(52) : 분사노즐 (53) : 펌프(52): injection nozzle (53): pump
(54) : 밸브 (60) : 유리판54: valve 60: glass plate
(111) : 설치홈 (111a): 배출홈(111): mounting groove (111a): discharge groove
(111b): 취출홈 (111c): 보조출구홈(111b): Outlet groove (111c): Auxiliary outlet groove
(112) : 출구홈 (113) : 배수구멍(112): outlet groove (113): drain hole
도 1 은 본 발명에 따른 구성을 보인 정면 예시도를, 도 2 는 본 발명에 따른 구성을 보인 평면 예시도를, 도 3 은 본 발명 회전판의 구성을 보인 예시도를 도시한 것으로, 본 발명은 미생물이 도말된 유리판(60)을 회전판 위에 장착시키고, 상기 유리판 일측 끝단에 염색액을 분무하거나 물을 분무하여 미생물을 염색시키거나 세척한 다음, 회전판을 회전시켜 탈수/건조하도록 되어 있으며, 열풍에 의해 회전판에 장착된 유리판에 도말되어 있는 미생물이 고정되도록 되어 있다.1 is a front view showing a configuration according to the invention, Figure 2 is a plan view showing a configuration according to the invention, Figure 3 is an illustration showing a configuration of the rotating plate of the present invention, the present invention The glass plate 60 is coated with microorganisms on the rotating plate, and one side of the glass plate is sprayed with a dye solution or water to dye or wash the microorganisms, and the rotating plate is rotated to dehydrate / dry the hot plate. As a result, the microorganisms smeared on the glass plate attached to the rotating plate are fixed.
즉, 본 발명은 미생물이 도말된 유리판이 장탈착되는 회전부재(10)와, 상기 회전부재(10)가 내부에 설치되는 지지부재(20)와, 상기 회전부재(10) 및 지지부재(20)에 각각 설치되어 회전부재(10)의 회전정도를 감지하는 감지부재(30)와, 상기 지지부재(20) 상부에 설치되는 가열부재(40)와, 상기 지지부재(20) 일측에 설치되는 분사부재(50)로 구성되어 있다.That is, the present invention is a rotating member 10 is a glass plate on which microorganisms are smeared, the support member 20 is installed inside the rotating member 10, the rotating member 10 and the support member 20 Installed on each side of the sensing member 30 for sensing the rotational degree of the rotating member 10, the heating member 40 installed on the support member 20, and one side of the support member 20. It consists of the injection member 50.
상기 지지부재(20)는 회전부재(10) 및 분사부재(50)의 일측이 내부에 설치되고, 가열부재가 상부에 설치되는 것으로, 가열부재(40), 분사부재(50) 및 회전부재(10)의 일측을 지지하는 몸체(21)와, 상기 몸체(21)내 상부에 형성되고 가열부재(40)와 연통되는 챔버(22)와, 상기 챔버(22)에 연결되어 지지부재(20) 외부와 연결되는 배수관(23)과, 상기 몸체(21) 상부에 설치되는 상부덮개(24)를 구비한다.The support member 20 is one side of the rotating member 10 and the injection member 50 is installed therein, the heating member is installed on the top, the heating member 40, the injection member 50 and the rotating member ( A body 21 supporting one side of the 10, a chamber 22 formed in the upper portion of the body 21 and in communication with the heating member 40, and connected to the chamber 22, the support member 20 It is provided with a drain pipe 23 connected to the outside, and an upper cover 24 installed on the upper body 21.
상기 회전부재(10)는 미생물이 도말된 유리판(50)을 탑재 및 회전시키는 것으로, 유리판(50)이 장/탈착되는 회전판(11)과, 상기 회전판(11)을 구동시키는 작동모터(12)로 구성되어 있다.The rotating member 10 mounts and rotates the glass plate 50 coated with microorganisms, the rotating plate 11 on which the glass plate 50 is mounted / removed, and an operation motor 12 driving the rotating plate 11. Consists of
상기 회전판(11)은 원판형상의 것으로, 원판 중심이 작동모터(12)의 축과 연결되어 지지부재(20)의 챔버(22)내에 설치되며, 상부에 유리판(50)이 장/탈착되는 다수개의 설치홈(111)과, 상기 회전판(11)의 중심을 중심으로 서로 다른 반경을 구비하도록 원주를 따라 형성된 다수개의 출구홈(112)과, 상기 출구홈(112)과 교차되도록 회전판(11)의 중심에서 원주를 향하는 방향으로 다수개의 배수구멍(113)이 형성되어 있다.The rotating plate 11 is a disk-shaped, the center of the disk is connected to the axis of the operating motor 12 is installed in the chamber 22 of the support member 20, a plurality of glass plate 50 is mounted / detached on the top Installation grooves 111, a plurality of outlet grooves 112 formed along a circumference to have different radii around the center of the rotating plate 11, and the rotating plate 11 to intersect the outlet grooves 112. A plurality of drainage holes 113 are formed in the direction from the center to the circumference.
상기 설치홈(111)은 유리판(50)이 설치되는 것으로, 장착되는 유리판(50)의 폭/길이와 동일한 폭/길이를 구비하고, 중앙에 길이방향으로 배출홈(111a)이 형성되어 있으며, 끝단에 취출홈(111b) 및 양측에 보조출구홈(111c)이 형성되어 있다.The installation groove 111 is a glass plate 50 is installed, has a width / length the same as the width / length of the glass plate 50 to be mounted, the discharge groove 111a is formed in the longitudinal direction in the center, The discharging groove 111b and the auxiliary outlet groove 111c are formed at both ends.
상기 배출홈(111a)은 유리판과 설치홈(111)내의 염색액을 배출하는 것으로,일측단이 취출홈(111b)에 연통되고, 또다른 일측단이 설치홈(111)에 유리판(50)이 장착될 시, 외부와 연통되도록 형성되어 있다. 즉, 상기 배출홈(111a)내에 고여 있는 염색액은 외부와 연통되는 일측단에 의해 외부로 배출된다.The discharge groove (111a) is to discharge the dye solution in the glass plate and the installation groove 111, one end is in communication with the extraction groove (111b), another one end is the glass plate 50 in the installation groove 111 When mounted, it is formed to communicate with the outside. That is, the dye solution accumulated in the discharge groove 111a is discharged to the outside by one side end in communication with the outside.
상기 취출홈(111b)은 유리판(50)을 용이하게 장/탈착할 수 있도록 형성한 것으로, 설치홈(111)의 끝단에 경사면을 구비하고 형성되어 있다.The extraction groove 111b is formed to easily mount / detach the glass plate 50, and is provided with an inclined surface at the end of the installation groove 111.
상기 보조출구홈(111c)은 배출홈(111a)과 같이 유리판(50)과 설치홈(111) 사이의 염색액을 외부로 배출하는 것으로, 설치홈(111)과 연통되도록 설치홈(111)의 양측에 일정간격을 유지하고 다수개가 형성되어 있다. 즉, 상기 설치홈(111)내에는 배출홈(111a)과 취출홈(11b) 및 다수개의 보조출구홈(111c)이 형성되어 있다.The auxiliary outlet groove 111c discharges the dye solution between the glass plate 50 and the installation groove 111 to the outside, such as the discharge groove 111a, and communicates with the installation groove 111 of the installation groove 111. Maintain a certain interval on both sides, and a number of them are formed. That is, the discharge groove 111a and the discharge groove 11b and the plurality of auxiliary outlet grooves 111c are formed in the installation groove 111.
상기 출구홈(112)은 회전판(11)의 설치홈(111)에 장착되는 다수개의 유리판 중, 유리판 사이와, 일측 유리판에 남아 있는 염색액이 또다른 유리판으로 넘어가는 것을 방지하는 것으로, 작동모터와 연결되는 회전판(11)의 중심을 중심으로, 서로 다른 반경을 구비하도록 또한, 일측 설치홈과 이에 근접하는 또다른 설치홈 사이에 위치하도록 다수개가 원주를 향하도록 형성되어 있으며, 반원형상의 홈을 구비한다. 즉, 상기 출구홈(112)은 회전판(11)을 상부에서 보았을 경우, 설치홈(111)에 형성된 보조출구홈(111c)과 어울려 원형을 이루도록 형성되어 있으며, 상기 출구홈(112)과 보조출구홈(111c)은 동일 깊이를 구비한다.The outlet groove 112 is to prevent the dye solution remaining in the glass plate between the glass plate and the glass plate of the plurality of glass plates mounted on the installation groove 111 of the rotating plate 11 to pass to another glass plate, the operation motor It is formed around the center of the rotating plate 11 to be connected to each other, having a different radius, and located between the one installation groove and another installation groove adjacent to the plurality of facing the circumference, the semi-circular groove Equipped. That is, the outlet groove 112 is formed so as to form a circle with the auxiliary outlet groove 111c formed in the installation groove 111 when the rotating plate 11 is viewed from the top, the outlet groove 112 and the auxiliary outlet The grooves 111c have the same depth.
상기 배출구멍(113)은 회전판(11)을 관통하여 형성되는 것으로, 회전판(11)에 원주방향으로 형성된 다수개의 출구홈(112)과 교차되도록 형성되어 있으며, 하나의 출구홈에 하나 이상의 배출구멍이 연통 및 교차되도록 또한, 설치홈과 설치홈사이에 하나이상의 배출구멍이 위치하도록 회전판(11)의 중심에서 원주방향으로 다수개가 형성되어 있다.The discharge hole 113 is formed through the rotating plate 11, is formed to cross the plurality of outlet grooves 112 formed in the circumferential direction in the rotating plate 11, one or more discharge holes in one outlet groove A plurality of circumferential directions are formed in the circumferential direction of the center of the rotating plate 11 such that at least one discharge hole is located between the installation groove and the installation groove so as to communicate and intersect.
상기 작동모터(12)는 회전판(11)의 중심과 연결되어 회전판(11)을 회전시키는 것으로, 지지부재(20)의 몸체(21)내에 설치되고, 챔버(22)내에 위치하는 회전판(11) 중심하부와 연결축(13)에 의해 연결된다. 이때, 상기 연결축은 몸체에 베어링 지지됨과 동시에, 챔버내의 물질이 연결축을 따라 흐르지 못하도록 실링처리되어 있다.The operating motor 12 is connected to the center of the rotary plate 11 to rotate the rotary plate 11, is installed in the body 21 of the support member 20, the rotary plate 11 located in the chamber 22 It is connected by the lower center and the connecting shaft (13). At this time, the connecting shaft is bearing-supported to the body, and is sealed to prevent the material in the chamber from flowing along the connecting shaft.
상기 감지부재(30)는 작동모터(12)에 의해 회전되는 회전판(11)의 회전정도를 감지/측정하는 것으로, 회전판(11)의 일측 하부끝단에 설치되는 자석(31)과, 지지부재의 챔버(22) 일측에 설치되어 자석(31)의 위치를 감지하는 자석센서(32)로 구성되어 있으며, 상기 작동모터(12)에 의한 회전판(11)의 회전시, 자석(31)의 위치를 자석센서(32)에 의해 감지하여, 작동모터(12)의 구동정도 및 회전판(11)의 회전정도(위치)를 감지하도록 되어 있다.The sensing member 30 detects / measures the degree of rotation of the rotating plate 11 rotated by the operating motor 12, and a magnet 31 installed at one lower end of the rotating plate 11 and a support member. It is composed of a magnet sensor 32 installed on one side of the chamber 22 to sense the position of the magnet 31, the rotation of the rotating plate 11 by the operating motor 12, the position of the magnet 31 Detected by the magnet sensor 32, it is to detect the driving degree of the operating motor 12 and the rotation degree (position) of the rotating plate (11).
상기 분사부재(50)는 염색액을 회전판(11)에 설치된 유리판(50)의 끝단에 분무하는 것으로, 염색액을 분무하는 다수개의 분사노즐(52)과, 상기 염색액이 저장되는 다수개의 약품통(51)과, 상기 다수개의 약품통(51)내 염색액을 각각의 분사노즐(52)로 이송시키는 다수개의 펌프(53)와, 상기 펌프(53)와 분사노즐(52) 사이에 설치되는 밸브(54)로 구성되어 있으며, 상기 펌프(53)와 밸브(54)는 지지부재(20)의 몸체(21)내에 설치되고, 분사노즐(52)의 끝단은 챔버(22)내에 위치하도록 설치된다.The spray member 50 is to spray the dye solution to the end of the glass plate 50 installed on the rotating plate 11, a plurality of spray nozzles 52 for spraying the dye solution, and a plurality of chemicals in which the dye solution is stored Cylinder 51, a plurality of pumps 53 for transferring the dyeing liquid in the plurality of chemical containers 51 to each injection nozzle 52, and installed between the pump 53 and the injection nozzle 52 The pump 53 and the valve 54 are installed in the body 21 of the support member 20, and the end of the injection nozzle 52 is located in the chamber 22. Is installed.
즉, 상기 염색액의 공급은 약품통(51)에 들어있는 염색액을 펌프(53)로 빨아들여 밸브(54)를 통해 분사노즐(52)로 원하는 양을 유리판(50)으로 보내도록 되어 있으며, 염색액 등을 유리판(50)에 뿌려줄 때, 회전판(11)에 설치된 자석(31)과, 챔버(22)에 설치된 자석센서(32)에 의해 작동모터(12)의 현위치값을 측정하고, 상기 측정값에 의해 염색하고자 하는 미생물이 고정된 유리판을 원하는 염색액을 분무하는 분사노즐의 중앙에 위치하도록 작동모터(12)를 작동하여 회전판(11)을 회전시키도록 되어 있다.That is, the supply of the dyeing solution is to suck the dyeing solution contained in the chemical container 51 into the pump 53 to send the desired amount to the injection nozzle 52 through the valve 54 to the glass plate 50. When the dyeing liquid is sprayed onto the glass plate 50, the current position value of the operating motor 12 is measured by the magnet 31 installed in the rotating plate 11 and the magnet sensor 32 installed in the chamber 22. And, by operating the operating motor 12 so as to be located in the center of the spray nozzle for spraying the desired dyeing solution to the glass plate to which the microorganism to be stained by the measured value is to rotate the rotating plate (11).
상기 가열부재(40)는 유리판(50)의 미생물 고정을 용이하게 하고, 또한 염색 반응을 촉지하기 위한 것으로, 챔버(22)내로 원하는 온도의 공기를 공급하여 주는 열풍기(41)가 내부에 설치되어 있으며, 상기 열풍기(41)에 의해 원하는 온도로 가열된 공기가 챔버(22)로 이동될 수 있도록 일측이 챔버(22)와 연통되게 지지부재(20)의 상부덮개(24) 상부에 설치된다.The heating member 40 is for facilitating the fixing of the microorganisms of the glass plate 50 and also for facilitating the dyeing reaction. A heating fan 41 for supplying air of a desired temperature into the chamber 22 is installed therein. In addition, one side is installed on the upper cover 24 of the support member 20 to communicate with the chamber 22 so that the air heated to the desired temperature by the hot air fan 41 is moved to the chamber 22.
상기와 같이 구성된 본 발명은 염색하고자 하는 미생물을 백금이에 의해 유리판에 도말하고, 상기 미생물이 도말된 유리판을 회전판의 설치홈에 장착 시킨 다음, 가열부재에 의해 원하는 온도의 공기를 챔버내로 공급하여 세균을 유리판에 고정하고, 회전부재에 의해 원하는 염색액을 분무하는 일측 분사노즐의 중앙에 유리판을 위치시킴과 동시에, 분사부재에 의해 유리판 일측 끝부분에 염색액을 뿌려 점차적으로 유리판 전체에 염색액이 퍼지되도록 하여 유리판에 고정된 미생물을 자동으로 염색하도록 되어 있다.In the present invention configured as described above, the microorganisms to be dyed are plated on a glass plate by platinum, and the microplates are plated on the installation groove of the rotating plate, and then, the heating member supplies air of a desired temperature into the chamber. The bacteria are fixed to the glass plate, the glass plate is placed at the center of one injection nozzle spraying the desired dye solution by the rotating member, and the dye is sprayed on one end of the glass plate by the injection member, and the dye solution is gradually applied to the entire glass plate. The microorganisms fixed on the glass plate are automatically stained by the purge.
상기와 같은 작동에 의해 유리판에 도말된 미생물의 염색이 완료되면, 회전부재에 의해 회전판을 회전시키어, 출구홈과 배출홈을 통해 회전판 외부로 염색액을 배출시켜 탈수 시킨다. 이때, 상기 일측 슬리이드 글래스의 염색액은 출구홈과 교차하도록 형성된 배수구멍을 통해 챔버로 이동되므로, 인접하는 또다른 유리판으로 염색액이 넘어가는 현상은 방지된다.When the dyeing of the microorganisms smeared on the glass plate by the operation as described above is completed, by rotating the rotating plate by the rotating member, the dye solution is discharged to the outside of the rotating plate through the outlet groove and the discharge groove to dehydrate. At this time, since the dye solution of the one side of the glass glass is moved to the chamber through the drain hole formed to cross the outlet groove, the phenomenon that the dye solution is passed to another adjacent glass plate is prevented.
이와 같이 챔버로 이동된 염색액은 배수관을 통해 지지부재 외부로 이송된다.In this way, the dye solution transferred to the chamber is transferred to the outside of the supporting member through the drain pipe.
본 발명은 상술한 특정의 바람직한 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 발명이 속하는 기술분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형실시가 가능한 것은 물론이고, 그와 같은 변경은 청구범위 기재의 범위내에 있게 된다.The present invention is not limited to the above-described specific preferred embodiments, and various modifications can be made by any person having ordinary skill in the art without departing from the gist of the present invention claimed in the claims. Of course, such changes will fall within the scope of the claims.
이와 같이 본 발명은 유리판 위에 도말된 미생물을 자동으로 고정/염색하도록 되어 있어, 짙은 염색 결과를 얻을 수 있으며, 염색시간을 단축하고, 검사의 정확도, 검사공정의 숙련도에 따른 변화감소 및 미숙련자 검사수행이 가능하다.As described above, the present invention is to fix / dye the microorganisms smeared on the glass plate automatically, so that a dark dyeing result can be obtained, the dyeing time can be shortened, the accuracy of the test and the change reduction according to the skill of the test process and the unskilled test It can be done.
또한, 염색공정시 발생되는 세균에 의한 2차 감염을 방지할 수 있으며, 검사환경이 청결하게 되고, 검사하기 위한 공간을 감소시킬 수 있다.In addition, the secondary infection by the bacteria generated during the dyeing process can be prevented, the inspection environment is clean, and the space for inspection can be reduced.
또한, 유리판 끝단에 염색액을 분무하여 전체로 퍼지도록 되어 있으므로, 분무되는 염색액으로 인해 고정된 세균이 떨어져 나가는 현상을 방지하였다.In addition, since the dye is sprayed on the glass plate ends to spread throughout, it prevented the fixed bacteria from falling off due to the sprayed dye solution.
또한, 다수개의 배수구멍과 출구홀 및 배수홀에 의해 염색액이 빠지도록 되어 있어, 회전부재에 의한 탈수작업이 용이하고, 유리판 간의 세균 이동을 방지할 수 있는 등 많은 효과가 있다.In addition, the dye solution is removed by a plurality of drainage holes, outlet holes and drainage holes, so that the dehydration operation by the rotating member is easy, and there are many effects such as preventing bacterial movement between glass plates.
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| JP2000232875A (en) * | 1999-02-15 | 2000-08-29 | Muto Kagaku Kk | Gram staining apparatus |
-
2001
- 2001-12-28 KR KR10-2001-0086625A patent/KR100425233B1/en not_active IP Right Cessation
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100736335B1 (en) * | 2005-12-20 | 2007-07-06 | 주식회사 다가 | Sample Heating Apparatus of Automated Stainer |
| KR101416966B1 (en) * | 2013-12-31 | 2014-07-09 | 주식회사 다가 | Automatic Dyeing Apparatus Capable of Sample Multi-Layer Loading |
| CN113834715A (en) * | 2020-06-23 | 2021-12-24 | 上海伯顿医疗设备有限公司 | Dyeing machine of many slide loads, pneumatic atomizing hydrojet |
| CN113834715B (en) * | 2020-06-23 | 2024-05-14 | 上海伯顿医疗设备有限公司 | Dyeing machine with multiple glass slide loading capacity and pneumatic atomization liquid spraying |
Also Published As
| Publication number | Publication date |
|---|---|
| KR100425233B1 (en) | 2004-03-31 |
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