KR20030037885A - Composition for elimination of hangover containing naringin - Google Patents

Abstract

PURPOSE: Provided is a composition containing, as an active ingredient, naringin which reduces a blood alcohol concentration and increases the reduced blood glucose level. Therefore, the composition is effectively used as a functional food or a pharmaceutical composition for removing hangover. CONSTITUTION: The functional food or pharmaceutical composition for removing the symptoms of hangover contains, as an active ingredient, a great quantity of naringin which is a bioflavonoid contained in a rutaceous plant (e.g., orange, grapefruit, lemon or Citrus UNSHIU). The composition additionally contains excipients, disintegrants, sweeteners, lubricants, flavorings or the like.

Description

Composition for elimination of hangover containing naringin {Composition for elimination of hangover containing naringin}

The present invention relates to a hangover releasing composition containing naringin, and more particularly to a hangover releasing composition containing naringin to reduce blood alcohol concentration and raise blood sugar lowered by alcohol consumption.

Recently, many people drink a lot of alcohol as part of social activities or as a way to relieve stress. The main component of alcohol is ethanol, which is ingested in all organs, including the liver, and some (about 10%) are excreted through breathing or into urine and sweat. In the liver, the conversion to acetaldehyde through oxidation is the main metabolism. This is known to proceed by three reactive enzyme systems: alcohol dehydrogenase (ADH), microsomal ethanol-oxidizing system (MEOS), and catalase (K. Ebihara et al., Agri). Biol. Chem., 52, 1311 (1988)). It is known that the poisonous action of acetaldehyde, which occurs during alcohol metabolism, causes heavy hangover after drinking alcohol, causing symptoms such as general boredom, fatigue, bloating and vomiting. The toxicity of ethanol has been reported to affect genetics as well as neurological aspects such as hangover (J. Caballerial, et al., Life Sci., 41, 1921-1727 (1986)). In recent years, alcohol consumption leads to a decrease in blood sugar, and it has been reported that the decrease in blood sugar causes hangover (Arthur S., et. Al., Alcohol, 13, 553-557 (1996), Daniel Bunout, Nutrition, 15, 583-589 (1999)

On the other hand, naringin is a bioflavonoid in citrus fruits that has been determined to be suitable by the US Food and Drug Administration for safety evaluation. The present inventors have conducted research on the use of naringin and disclosed that it has a cardiovascular disease prevention and treatment effect in Korean Patent Registration No. 213895. In Korean Patent Registration No. 213899, hydroxymethyl glutaryl-coay It has been disclosed that there is an effect of inhibiting reductase activity, and in Korean Patent Registration No. 258584, it has been disclosed that there is activity to inhibit acylcoa: cholesterol-ortho-acyltransferase.

The inventors of the present invention while exploring a new use of naringin (naringin) by adding naringin to the alcohol and non-alcohol diet group to measure the blood sugar level, blood alcohol concentration, etc., alcoholic hypoglycemic symptoms are improved and blood alcohol concentration is reduced It was confirmed that the hangover is effective in completing the present invention.

It is an object of the present invention to provide a functional food and pharmaceutical composition which reduces narcotic-containing blood alcohol concentration and improves alcoholic hypoglycemia so as to relieve hangovers.

Hereinafter, the configuration and operation of the present invention.

Figure 1 shows the effect of narinjin for 6 weeks on the weight of the rats of the alcohol diet group and non-alcoholic diet group.

Figure 2 shows the effect of naringin supplement on the blood glucose concentration of the rats of the alcohol diet group and non-alcoholic diet group.

(Average of 10 rats,

significant difference (p <0.05) between the different numbers of ^abc alcohol diets or non-alcoholic counterparts,

* Significant differences from corresponding alcohol diets (* p <0.05, ** p <0.01, *** p <0.005))

Figure 3 shows the effect on the blood alcohol concentration of naringin.

(Average of 10 rats,

significant difference between the different numbers in the ^abc alcohol diet (p <0.05),

ND not detected)

In the present invention, there is provided a hangover composition comprising naringin as an active ingredient with a pharmaceutically acceptable carrier. In addition, there is provided a functional food composition for hangover, including naringin.

Naringin may be extracted from citrus fruits or synthesized by known synthetic methods. The citrus fruits refer to a mandarin orange, sugar citron, orange, lemon, citrus fruit, grapefruit or citron. Flavonoids such as naringin in citrus fruits may vary depending on the maturity of the fruit or the type of fruit, respectively.The extraction method of naringin in citrus fruits can be found in the literature and patents of Merck Index et al. Many are open to the public.

Naringin is effective in relieving low blood sugar caused by alcohol intake by increasing blood sugar, and reducing hangover by reducing blood alcohol concentration by ingesting alcohol. Toxicity studies to date have shown that naringin has little toxicity when administered orally to rats at a dose of 1000 mg / kg. In addition, naringin has no adverse effects on the function of organs, including the liver.

Naringin can be prepared as an active ingredient by mixing with a carrier, which is pharmaceutically or as a food additive, to prepare a hangover composition. The composition may further include conventionally used excipients, disintegrants, sweeteners, lubricants, flavoring agents and the like, and may be tablets, capsules, powders, granules, suspensions, emulsifiers, syrups, solutions or by conventional methods or It may be formulated in a unit dosage form or in multiple dosage form pharmaceutical preparations, such as preparations for parenteral administration.

Hangover composition containing naringin of the present invention as an active ingredient can be parenterally or orally administered as desired, an amount of 0.001 to 1 g, preferably 0.01 to 0.1 g per kg of body weight per day It may be administered in divided doses of 1 to several times. Dosage levels for a particular patient may vary depending on the patient's weight, age, sex, health condition, diet, time of administration, mode of administration, absorption, severity of the disease, and the like.

Naringin may also be added to foods or beverages for the purpose of eliminating hangovers. Foods to which naringin can be added for the development of dietary supplements include, for example, various foods, meats, beverages, chocolates, snacks, confectionery, pizzas, ramen noodles, other noodles, gums, ice creams, alcoholic beverages, vitamins. Complexes, dietary supplements, and the like.

Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following Examples are only for illustrating the present invention, but the scope of the present invention is not limited to these.

Example 1 Animal Composition of Naringin with Dietary Composition and Alcohol of Experimental Animals

Sixty-four male rats of Sprague-Dawley (SD) series, aged four weeks, were sold from Bio-Genomics, Inc. Breed on a chow pellet diet, then divide the diet into six groups and feed the diet for six weeks. Animal test group was naringin-free alcohol diet group, 0.02% naringin-added alcohol diet group, 0.05% naringin-added alcohol diet group and the corresponding naringin-free alcohol-free diet group, 0.02% naringin-free alcohol-free diet group and 0.05% naringin addition group It consists of a non-alcoholic counterpart. The specific composition of the alcohol diet group consisting of fatty liver induction diet is alcohol (36% energy), high fat (35% energy) and other general dietary components, the corresponding alcohol-free diet group is the same energy consumed by alcohol diet group It is a group to eat from the normal diet. The diet of the experimental group is prepared daily and supplied twice a day to maintain freshness. The dietary composition of the specific experimental group is shown in Table 1 below. Naringin added to the diet was purchased from Sigma.

Alcohol (express alcohol or alcohol consistently) For adaptation of the diet, the alcohol content of the first and second days of feeding is 3% of the diet (21% of the total energy intake), and the alcohol content of the third and fourth days is 4 % (28% of the total energy intake), and from the fifth day to 5% of the diet (36% of the total energy intake).

Animal breeding experiments are conducted in experimental animal facilities with constant temperature and humidity. Liquid diets, including alcohol, are provided in specially designed liquid diet bottles. Animals are housed in individual stainless cages installed in animal laboratories that maintain a constant temperature (20 ^o C) and humidity (50%) and a light cycle from 7:00 to 19:00. Animals should observe and record specifics during the breeding period. Body weights are measured every three days and dietary intake is measured daily during the breeding period. Animals are intramuscularly injected (75 mg / kg body weight) with a Ketamine-HCl anesthetic after 15 hours of fasting to collect blood from the inferior vena cava and collect all organ tissues.

Composition of Experimental Diet (g / ℓ) Alcohol diet Alcohol-Free Diet Naringin Free Naringin 0.02% Narigin 0.05% Naringin Free Naringin 0.02% Naringin 0.05% Casein 41.4 41.4 41.4 41.4 41.4 41.4 L-cystein 0.5 0.5 0.5 0.5 0.5 0.5 DL-Methionine 0.3 0.3 0.3 0.3 0.3 0.3 Corn oil 8.5 8.5 8.5 8.5 8.5 8.5 Olive oil 31.1 31.1 31.1 31.1 31.1 31.1 Dextrin Maltose 25.6 25.6 25.6 115.2 ^a 115.2 115.2 Choline bitartrate 0.53 0.53 0.53 0.53 0.53 0.53 Fiber 10.0 10.0 10.0 10.0 10.0 10.0 Xanthan gum 3.0 3.0 3.0 3.0 3.0 3.0 Vitamin mix ^b 2.55 2.55 2.55 2.55 2.55 2.55 Mineral mix ^c 9.0 9.0 9.0 9.0 9.0 9.0 Ethanol 50 50 50 - - - Naringin - 0.05 0.125 - 0.05 0.125 ^a 25.6 g of dextrin maltose in an alcoholic diet and 50 g of alcohol were replaced by 115.2 g of dextrin maltose in an alcohol-free diet (equivalent amount of energy) ^b Vitamin mixture by AIN-76. ^c Mineral mixture by AIN-76.

As a result of observing basic clinical symptoms such as weight, activity, and behavioral abnormalities for each experimental animal, the alcohol diet group showed less activity or movement and showed neurological sensitivity, but showed no specific external symptoms during the diet. No clinical symptoms were observed. In addition, as shown in FIG. 1, weight gain was observed in all the experimental groups during the entire experimental growth, but the average daily weight gain was significantly lower (p <0.005) in the alcohol diet group than in the non-alcoholic diet group. In addition, as shown in Table 2, naringin addition did not affect the daily weight gain and dietary intake of the alcohol diet group and non-alcoholic counterparts. The decrease in weight gain caused by alcohol is considered to be due to the fact that more than 10% of the energy consumed by alcohol is consumed by heat as a heat-generating reaction in the "futile cycle" reaction.

In addition, after the end of the experiment to test the animals after the autopsy to measure the weight change of the long-term weight to determine the weight change is shown in Table 2 below.

Effects of Naringin Supplementation on Daily Weight Gain, Dietary Intake, and Organ Weight in Alcoholic and Alcohol-Free Groups (Average of 10 Rats) Ethanol diet Pair-fed normal diet Narinjin additives Narinjin0.02% Narinjin0.05% Narinjin additives Narinjin0.02% Narinjin0.05% Daily weight gain (g / day) 3.86 ± 0.17 3.44 ± 0.21 3.92 ± 0.19 5.42 ± 0.08 ^*** 5.34 ± 0.09 ^*** 5.27 ± 0.12 ^*** Dietary Intake (ml / day) 73.89 ± 1.76 72.67 ± 1.55 72.30 ± 1.96 76.06 ± 0.06 74.69 ± 0.04 74.28 ± 0.01 Organ Weight (g / 100g B.W.) liver 3.43 ± 0.03 3.43 ± 0.1 3.31 ± 0.03 3.07 ± 0.05 ^*** 2.92 ± 0.08 ^*** 2.95 ± 0.06 ^*** Heart 0.32 ± 0.005 0.33 ± 0.003 0.31 ± 0.006 0.30 ± 0.006 ^** 0.30 ± 0.003 ^*** 0.30 ± 0.004 kidney 0.72 ± 0.01 0.72 ± 0.01 0.75 ± 0.01 0.66 ± 0.01 ^*** 0.67 ± 0.02 ^** 0.66 ± 0.02 ^*** Significant difference (p <0.05) * from the ^abc alcohol diet or other numbered nonalcoholic equivalent diet * Significant difference from the corresponding alcohol diet group (* p <0.05, ** p <0.01, *** p < 0.005)

The liver weight per 100g body weight was significantly higher in the alcohol diet group than in the alcohol-free diet group. Kidney weight per body weight was also significantly higher in the alcohol diet group than in the non-alcoholic diet group. Heart weight was higher in the alcohol diet group than in the 0.05% naringin supplement group. This suggests that alcohol administration is associated with increased lipid peroxide production in the heart (Lee et al., 1991) and liver tissue damage and tissue changes due to damage to the cell membrane (Sherwood et al. 1954). The naringin addition effect was observed to decrease liver weight somewhat, and did not affect other organ weights.

Example 2 Effect of Naringin Supplemented on Alcohol Diet on Blood Glucose Levels

Blood glucose levels were measured to investigate the effects of alcohol intake on glucose metabolism. Glucose reacts with enzymes and water in solution by the action of GOD (glucose-oxidase) to form gluconic acid and hydrogen peroxide. The produced hydrogen peroxide is produced by the action of peroxidase (phenol) and 4-amino. Antipyrine (4-aminoantipyrine) is oxidatively condensed to produce a quinone type red pigment. The red pigment was measured for absorbance at a wavelength of 500 nm to quantify the amount of glucose, and the results are shown in FIG. 2 using a kit of Asan Pharmaceutical, commercialized using this principle.

As shown in FIG. 2, all the alcohol diet groups had a significant (p <0.005) decrease in plasma glucose (p <0.005) compared to the non-alcoholic counterpart groups, confirming hypoglycemic reduction due to chronic alcohol intake. Naringin supplementation increased the blood glucose level in the alcohol and nonalcoholic diets, especially in the 0.05% naringin supplement group.

Example 3 Effect of Naringin Supplemented with Alcohol Diet on Blood Alcohol Concentration

Blood alcohol levels are analyzed on a Cobas Integra 800 (Roche, Switz) instrument using ETOH (Roche, Switz) reagents. Principle of analysis is that NADH is produced by adding alcohol dehydrogenase (ADH) to alcohol, quantifying alcohol concentration by measuring the absorbed NADH, and the results are shown in FIG. 3.

As shown in FIG. Serum alcohol levels were maintained at 15 mg / dl after 6 weeks of chronic alcohol intake, but the 0.02% and 0.05% naringin supplemented groups had significantly higher alcohol concentrations of 23% and 47%, respectively, than the naringin-free alcohol diet group. Reduced. In particular, the higher the amount of naringin, the lower the blood alcohol concentration. This phenomenon may be related to the improvement of alcohol metabolism efficiency.

As described in the above embodiment, the composition of the present invention containing naringin can be usefully used as a functional food or pharmaceutical composition for hangover resolution because it increases blood sugar lowered by alcohol intake and decreases blood alcohol concentration.

Claims (3)

Hangover releasing composition comprising naringin as an active ingredient. According to claim 1, The naringin is hangover composition, characterized in that to lower the blood alcohol concentration. According to claim 1, wherein naringin is a hangover composition, characterized in that to increase the blood sugar concentration.