KR20030019724A - Culture device for probiotic fermentation using livestocks - Google Patents

Abstract

PURPOSE: A device for fermenting probiotic for domestic animals is provided, thereby a large quantity of culture media can be stably contained in a fermenting bag, to provide the optimal fermentation condition and to effectively supply oxygen to the culture media. CONSTITUTION: The device for fermenting probiotic for domestic animals comprises a fermenting bag(10) containing a culture medium(60) in each culturing chamber(50); a conventional ventilation apparatus(20) with a ventilation fan(21) outside the culturing chamber(50); an oxygen-supplying pipe(26) having opening and shutting valves(27), fitted to the outside of the culturing chamber(50) and connected to the ventilation apparatus(20) to supply the culturing chamber with oxygen; and oxygen-supplying hoses(28) connected to each opening and shutting valve(27), and connected to the culturing vessel(50) and to the fermenting bag(10) through an air-inlet pin(32) having a plurality of holes.

Description

Culture device for probiotic fermentation using livestocks}

The present invention relates to a culture apparatus for probiotic fermentation for livestock, and more particularly, to provide a fermentation bag that satisfies optimal fermentation conditions by safely accepting a large amount of a culture mixed with a culture material and a spawn, It is an object to improve the quality and mass production by providing a culture apparatus that can effectively supply the oxygen required for the culture contained in the fermentation bag.

Probiotics, as opposed to antibiotics, have the function of intestinal microorganisms to help digestion, prevent digestive diseases, and have excellent effects in removing odors such as ammonia and hydrogen sulfide gas from barns. In particular, probiotics fed to livestock must survive and reach the intestine of the livestock, and must also function to settle in the intestine.

Currently, probiotic, which ingests beneficial microorganisms in the intestines of animals and suppresses pathogenic microorganisms, is widely used in domestic livestock farms.In the method of manufacturing such probiotics, microorganisms are fermented in liquid form and mixed with excipients and then dried. Liquid fermentation and cooji fermentation process is generally used.

Liquid fermentation and coozy fermentation is a process for producing probiotics in which enzymes and metabolites produced by microorganisms remain in culture, that is, by fermenting them into complex microbial strains that open their shells naturally. It produces chitin, chitosan, and chitooligosaccharides, which are much more economical than conventional methods of obtaining chitosan by removing and deacetylating protein, calcium and pigments with chemicals such as HCL, NaOH, and ethanol. At the same time, it is widely used as a way to solve environmental waste during processing in that crab shell can be used as a 100% feed additive.

In this conventional manufacturing process, the culture and probiotic using soybean meal, rice cake, rice bran, crab shell, etc. as raw materials are mixed in a mixing device, mixed evenly, and then a certain amount is dispersed in a plurality of culture vessels and oxygen can be spread evenly. Spread as thin and evenly as possible to accommodate. At this time, the culture vessel is formed of a structure that can be stacked in a large number of vent holes are formed on the outer surface and a rectangular rectangular box shape with an open upper surface, and stored in each culture chamber in the form of up, down, left, right. The ventilator provided in the culture chamber is periodically ventilated to supply fresh air for cultivation to perform fermentation.

However, conventionally, the structure of the culture vessel does not meet the conditions for the culture to ferment optimally, so that the constant temperature and oxygen are not properly supplied, so that the enzymes and metabolites produced by the microorganisms do not exist in the culture. There was a problem that this greatly reduced. In addition, manpower is mobilized from the work of putting the culture to the culture vessel to the storage and recovery of the culture chamber. The culture chamber is evacuated through the air vents and contaminated in the culture chamber. In particular, the culture contacts the pathogenic microorganisms in the air and reuses the recovered culture vessel, including in the culture chamber contaminated by the fungus propagated during fermentation. In order to delay the cultivation work due to various post-processing operations such as cleaning and sterilization, mass production could not be expected due to the inability to continuously operate the culture chamber for a certain period of time.

The present invention provides a fermentation bag in the form of a large-sized bag that can accommodate a larger amount of culture in a safe state. In addition to eliminating all post-production work, the company aims to reduce manpower and mass production by establishing efficient management tasks using transportation equipment from storing and retrieving this massive fermentation bag to the culture chamber. Providing a culture device that can effectively supply oxygen required for fermentation to the culture to promote the growth and activation of microorganisms in the culture, and high quality products in which the enzymes and metabolites produced by these microorganisms remain in the culture. To mass-produce.

1 is an overall configuration according to the present invention

Figure 2 is an enlarged perspective view showing the air injection pin according to the present invention

<Description of the symbols for the main parts of the drawings>

10: fermentation bag 10 ': disposable endothelial vinyl

20: blower 21: blower fan

22: power supply 24: antibacterial filter

26: oxygen supply pipe 27: on-off valve

28,28 ': Oxygen supply hose 30: "T" type connection port

32: air injection pin 33: discharge hole

50: culture chamber M: motor

The present invention stores the fermentation bag (10) containing the culture (60) in each culture chamber 50 arranged in a row in a compartment structure, and by the blower fan 21 driven by a motor (M) outside the culture chamber Oxygen supply pipe (26) provided with a conventional blower (20) for sucking and blowing external air, which is provided to block and supply oxygen by the on / off valve (27) along the front outer wall of the culture chamber (50). On the other hand, by connecting the oxygen supply hose 28 to each of the on-off valves, one end of the lead into the culture chamber 50, and configured, the end of the rod-shaped air injection pin 32 having a plurality of discharge holes on the outer surface It is characterized in that it is configured to be inserted into the fermentation bag (10) by connecting, fixing.

Fermentation bag (10) is a disposable bag endothelial vinyl (10 ') to maintain a constant fermentation temperature required for the culture by receiving the culture substantially in the form of a large bag that can accommodate a large amount of culture in the inner surface This disposable endothelial vinyl (10 ') is separated from the fermentation bag (10) after use, discarded and replaced with a new disposable endothelial vinyl so that it can be reused to make the fermentation bag more hygienic and clean. To be used.

In another embodiment, the present invention connects and fixes the "T" type connection port 30 to the end of the oxygen supply hose 28 and connects the oxygen supply hose 28 'to each of the culture chambers 50, respectively. It is also preferable to be configured to be drawn, configured.

The blower 20 is a conventional blower system that sucks and blows external air by a blower fan 21 driven by a motor M therein. An antibacterial filter 24 is selectively provided on a suction inlet side for sucking outside air. It is recommended to filter the harmful microorganisms and contaminants contained in the external air to supply more clean oxygen to the culture.

Oxygen supply hose (28) is a flexible synthetic resin material is free to bend and can be extended and configured according to the installation conditions, the "T" type connection port 30 is fixed to the end, and there is a separate oxygen supply hose Each 28 'is connected and connected to each other, and when the culture chambers 50 are continuously installed in parallel, the oxygen supply hoses 28' are respectively introduced into both culture chambers.

In addition, the motor M is connected to the power supply 22 to receive power, and is preferably configured to operate the motor intermittently for a predetermined time by a timer mounted on the power supply.

The electrical connection system is a technical configuration for the main building which can be configured by those skilled in the art through easy design changes, the present invention will not be described in detail.

Air injection pin 32 is a long rod-like outer surface of the plurality of discharge holes 33 are formed at regular intervals in the longitudinal direction and the end is formed in a pyramid to penetrate the outer shell of the fermentation bag 10 so as to be easily inserted into the inside. It was.

Hereinafter, specific actions and effects on the culture apparatus for fermentation of probiotic for livestock according to the present invention will be described with reference to FIGS. 1 and 2, and reference numeral 60 denotes a culture.

The fermentation bag 10 is provided with a large number of cultures 60 and a mixture of the culture material and the spawn evenly through a compounding device (not shown), respectively. It is stored in each culture chamber 50 by using a transport equipment. At this time, in order to convey the fermentation bag using a transport equipment, but not shown in the drawing is provided with a pallet for transport.

The present invention can be easily stored by transporting the fermentation bag to a higher point by using a transport equipment, it is possible to install a culture chamber in more than a single layer, thus expanding more culture chambers as needed for the existing fermentation facilities In particular, it will be possible to collectively perform management tasks for storage and retrieval using transport equipment, thereby improving work efficiency and mass production.

On the other hand, after the storage of the fermentation bag 10 in the culture chamber, the blower fan 21 sucks the external air by the rotational force by the operation of the motor (M) receiving the power of the power supply unit 22. At this time, the outside air in which the various pathogenic harmful microorganisms and foreign substances contained in the outside air are filtered by the antibacterial filter 24 built in the inlet side is connected to the outlet side by the blowing force of the blower fan, and connected to the oxygen supply pipe 26. It is supplied along, through the oxygen supply hose 28 through the selective opening operation of the opening and closing valve 27 configured at the point located in the partition of each culture chamber 50 to the outer surface of the air injection pin 32 Oxygen is released through the discharge hole 33 formed. At this time, the operation of the on-off valve 27 may be selectively opened and closed depending on whether or not the operation of the culture chamber.

In such a state, the manager inserts the air injection pin 32 into the fermentation bag 10 so as to penetrate deeply into the inside of the fermentation bag 10 so that the insertion position is as close as possible to the bottom of the fermentation bag 10. Good to do. That is, in order to allow the oxygen to evenly contact the entire culture accommodated in the fermentation bag by the buoyancy of oxygen released through the discharge hole 33 of the air injection pin (32). And the oxygen supply to the culture contained in the fermentation bag is a periodic supply is made by the intermittent operation of the motor by the timer and the oxygen supplied to the fermentation bag 10 is carried out in the form that is released into the atmosphere through the open top do.

After completion of the normal fermentation for a certain period of time, the fermentation bag 10 is taken out of the culture chamber 50 by using a transport equipment to recover the culture 60 contained in the disposable endothelial vinyl 10 'and The collected culture is put into a drum dryer (not shown), dried with water to be 15% or less, and then weighed and packaged to produce a product. The disposable endothelial vinyl (10 ') is then used, and then easily separated from the fermentation bag (10) to be discarded and replaced with a new disposable endothelial vinyl so that the fermentation bag can be reused continuously in a more hygienic and clean state. To make it possible.

In order to commercialize the final culture after the completion of such fermentation, a microbial water test, which is usually performed regularly, is performed to determine a shelf life, and some samples of the culture obtained by the embodiment of the present invention are used. The results of analyzing the number of microorganisms collected are shown in the table below.

<Example>

<Analysis of Microbial Counts by Traditional Koji Fermentation>

After mixing before incubation After 1 week incubation After drying (finished product) Lactobacillus 5.0 × 10 ⁷ cfu / g 8.5 × 10 ⁸ cfu / g 1.0 × 10 ⁸ cfu / g Bacillus 8.0 × 10 ⁷ cfu / g 2.5 × 10 ⁹ cfu / g 1.0 × 10 ⁹ cfu / g Yeast mold 1.0 × 10 ⁷ cfu / g 7.0 × 10 ⁷ cfu / g 5.0 × 10 ⁷ cfu / g leaven 5.0 × 10 ⁶ cfu / g 1.0 × 10 ⁸ cfu / g 5.0 × 10 ⁷ cfu / g Photosynthetic microorganisms 6.0 × 10 ⁶ cfu / g 8.5 × 10 ⁷ cfu / g 5.0 × 10 ⁷ cfu / g

<Analysis Result of Microorganisms by Koji Fermentation According to the Present Invention>

After mixing before incubation After 5 days incubation After drying (finished product) Lactobacillus 5.0 × 10 ⁷ cfu / g 1.0 × 10 ⁹ cfu / g 5.0 × 10 ⁸ cfu / g Bacillus 8.0 × 10 ⁷ cfu / g 5.0 × 10 ¹⁰ cfu / g 8.0 × 10 ⁹ cfu / g Yeast mold 1.0 × 10 ⁷ cfu / g 2.0 × 10 ⁸ cfu / g 1.0 × 10 ⁷ cfu / g leaven 5.0 × 10 ⁶ cfu / g 8.0 × 10 ⁸ cfu / g 2.0 × 10 ⁷ cfu / g Photosynthetic microorganisms 6.0 × 10 ⁶ cfu / g 1.5 × 10 ⁸ cfu / g 1.0 × 10 ⁷ cfu / g

As shown through the comparison table, the present invention is to effectively supply oxygen to the culture contained in the fermentation bag through the air injection pin and to evenly contact the entire culture to actively promote the activation of microorganisms, and also disposable endothelial vinyl By maintaining the internal temperature of the fermentation bag by satisfying the optimum fermentation conditions necessary for the culture of microorganisms it was found that the number of microorganisms significantly increased, as well as the fermentation time was reduced by about 2 days. .

As described above, the present invention increases the number of microorganisms by stably accommodating the cultures without spilling by fermentation bag to maintain the cleanliness of the culture chamber and at the same time satisfying the optimum fermentation conditions for the cultures stored in large quantities. And it is possible to mass-produce a better product by promoting the activation. In addition, it is possible to promote mass production by excluding the margin period due to post-fermentation work after fermentation work as usual, and to promote mass production, and to store and recover fermentation bags using transport equipment. By constructing efficient management work up to the cost of manpower can be greatly reduced.

Claims (3)

The fermentation bag 10 containing the culture 60 is stored in each of the culture chambers 50 arranged in a row in a compartment structure, and the outside air is blown by a blower fan 21 driven by a motor M outside the culture chamber. It is provided with a conventional blower 20 for sucking and blowing, which is connected to the oxygen supply pipe 26 installed to supply and block the oxygen by the on-off valve 27 along the front outer wall surface of the culture chamber (50) On the other hand, by connecting the oxygen supply hose (28) to each of the on-off valves, the one end is introduced into the culture chamber 50, and the end is connected to the rod-shaped air injection pin 32 having a plurality of discharge holes on the outer surface, Culture device for fermentation of probiotic for livestock, characterized in that it is fixed to be inserted into the fermentation bag (10) inside. According to claim 1, Fermentation bag 10 is a livestock probiotic, characterized in that the disposable endothelial vinyl (10 ') is accommodated so as to maintain the fermentation temperature to substantially accommodate the culture on the inner surface in a large bag shape Incubator for fermentation. The method of claim 1, wherein the "T" type connection port 30 is connected to and fixed to the end of the oxygen supply hose 28, and the oxygen supply hose 28 'is connected to each of the incubation chambers 50 to draw in, Culture apparatus for fermentation of probiotic for livestock, characterized in that configured.