KR20030014052A - Composition of natural bio-deodorant - Google Patents

Abstract

PURPOSE: A biodeodorizing agent composition containing a culture solution, a pyroligneous solution, a small amount of flavorings and purified water is provided which is harmless to a human body, live stock and plants and has excellent antimicrobial activity against putrefactive or pathogenic bacteria and a long-lasting deodorizing effect. CONSTITUTION: The biodeodorizing agent composition comprises a microorganism culture solution containing a Bacillus strain which has organic matter decomposability and forms endospore, a pyroligneous acid solution, a small amount of flavorings and purified water. The Bacillus strain has a final concentration of 0.5x10¬7 to 1x10¬7, based on 3 to 5% pyroligneous solution.

Description

Natural biodeodorant composition {COMPOSITION OF NATURAL BIO-DEODORANT}

The present invention relates to a natural biodeodorant composition, and more particularly, to a new natural biodeodorant composition which is composed of a natural plant extract having excellent efficacy in removing odors and a microbial group excellent in decomposing organic matter. .

In general, a lot of smell or bad smell is mainly used a lot of fragrance, but rather than removing the source of the smell or bad smell mainly masked by a stronger aroma effect and sensory neutralization of sensory deodorization.

In addition, the chemical deodorant may be harmful to humans, livestock, and plants, and in the case of a single natural deodorant, the deodorizing effect is not sustained.

An object of the present invention is to solve the above problems, to compensate for the shortcomings of the conventional chemical deodorant or single natural deodorant has a continuous and effective deodorizing ability and also excellent antibacterial activity against rot and pathogenic bacteria It is to provide a composition of natural biodeodorant.

Figure 1 illustrates the efficiency of removal of odor components by natural biodeodorant.

Therefore, the natural biodeodorant composition according to the present invention for achieving the above object of the present invention is a microbial group culture medium consisting of strains of the genus Bacillus excellent in organic decomposition and forming endospores, wood vinegar extracted from natural plants, and a small amount of fragrance and Includes purified water.

Here, the cultured Bacillus strain is preferably a final concentration of 0.5 × 10 ⁷ ~ 1 × 10 ⁷ CFU / ml for 3 to 5% of the wood vinegar solution.

Hereinafter, a natural biodeodorant composition according to the present invention will be described in detail with reference to the accompanying drawings.

The natural biodeodorant composition according to the present invention is completed by adding a microorganism group having excellent organic degradability to wood vinegar, which is a natural plant extract known to be excellent in deodorizing ability, and adding a small amount of perfume and purified water to the additive.

At this time, looking at the method of culturing and adding the microbial strain, three kinds of Bacillus strains that form strong endogenous spores having excellent resistance to external stimulation from the external stimulus among the microorganisms having excellent organic decomposition ability and at the same time, and cultured in a strain culture medium Let's do it.

Herein, the strain culture medium is as follows: Modified YPD broth. Yeast extract 5g / l, peptone 5g / l, glucose 10g / l, MgSO ₄ · 7H ₂ O 1g / l, CaCl ₂ · 2H ₂ O 0.08g / l, FeCl ₂ · 6H ₂ O 8.3mg / l, MnCl ₂ 4H ₂ O 1.4 mg / l, Na ₂ MoO ₄ 1.H ₂ O 1.17 mg / l, ZnCl ₂ 1 mg / l.

Each of the three strains was inoculated into the medium and incubated at 160 rpm in a shaker at 30 ° C. for 48 hours. Each cultured strain was added to the final concentration of 0.5 × 10 ⁷ ~ 1 × 10 ⁷ cfu / ml for purified 3-5% of the wood vinegar. Most preferably, the strain is one having a final concentration of 1 × 10 ⁷ CFU / ml for 5% wood vinegar.

Example 1 Deodorization Test

The tester uses Model 801 (Gastec co, Japan) and the experimental conditions are as follows.

1) Room temperature: 21 ℃

2) Relative Humidity: 52.0%

3) Size of container (container for deodorization test): 40cm × 40cm × 60cm

4) Sample amount: 20ml of sample

Firstly, the odor source is injected into the deodorization test apparatus and measured by gastec so that the malodorous methyl mercaptan in the device is 50ppm, hydrogen sulfide, ammonia, and amine (trimethylamine) are 60ppm, respectively. A sample of 150 times diluted deodorant stock is sprayed through an injector into each device adjusted to the initial concentration. 30 minutes, 1 hour, 2 hours, 4 hours, 6 hours after the injection, each concentration is measured by gastec.

The results are shown in Table 1.

Deodorization ability test result of natural biodeodorant Examination Elapsed time (hr) Analysis result (ppm) ammonia Hydrogen sulfide Trimethylamine Methyl mercaptan (NH ₃ ) (H ₂ S) ((CH ₃ ) ₃ NH ₂ ) (CH ₃ SH) Deodorization test 0 60 60 60 60 0.5 22 41 20 48 One 20 37 20 48 2 19 32 18 48 4 17 30 13 45 6 11 25 12 42

As shown in Table 1, the results of the deodorizing ability test of the natural biodeodorant of the present invention showed that the deodorizing ability of acid sulfides, hydrogen sulfide and methyl mercaptan, was 58.3% and 16%, respectively, compared to the initial concentration after 6 hours. After 6 hours, the deodorizing power of odorous ammonia and trimethylamine was 76.7% and 80% higher than the initial concentration.

<Example 2> Preservation test of organic decomposition microorganisms

LB agar plate (pancreatic digest of casein 10g / L, yeast extract 5g / L, NaCl 5g / over time while storing the natural biodeodorant of the present invention containing spores obtained by culturing as mentioned above at room temperature L, agar 15g / L) and the number of viable cells in the sample was measured.

Comparative Example 1 Antimicrobial Activity against Decay and Pathogenic Bacteria

E. coli, Salmonella sp., Staphylococcus aureus, Vibrio sp. It was tested whether to inhibit the growth of. Four strains incubated in LB broth were inoculated at about 10 ⁶ cfu / ml in each natural biodeodorant, and the number of viable cells was measured according to the above method as time passed.

In Comparative Example 1 as in Example 2. Referring to the test results, 0.5X10 ⁷ cfu / ml was added to the Bacillus sp pojasu three kinds of natural bio deodorant is in the initial concentration of 1X10 ⁷ cfu / ml at 30 ℃ for 6 months Showed excellent viability.

On the other hand, the inhibition of growth of the above four decaying and food poisoning bacteria by the natural biodeodorant showed a mortality of more than 99.9% of the initial number of bacteria by vividly dropping the viable count from the first day.

As mentioned above, the natural biodeodorant composition according to the present invention is effective to remove odor components by using wood vinegar which is a natural plant extract that can remove odor components and microorganism spores excellent in organic decomposition and resistant to the external environment. It is a very useful invention for the environmental and chemical industries related to the manufacture of deodorants as it can be removed continuously and continuously and exhibits excellent antibacterial effect against rot and pathogenic microorganisms.

On the other hand, while the above has been described with reference to the preferred embodiments of the present invention, those skilled in the art to which the present invention pertains without departing from the spirit and scope of the present invention described in the claims below It will be understood that modifications and changes can be made.

Claims (2)

A natural biodeodorant composition comprising a microorganism culture medium composed of Bacillus sp. Strains that have excellent organic decomposition and form endogenous spores, wood vinegar extracted from natural plants, and a small amount of perfume and purified water. The natural biodeodorant composition of claim 1, wherein the cultured Bacillus sp. Strain has a final concentration of 0.5 × 10 ⁷ to 1 × 10 ⁷ CFU / ml for 3 to 5% of wood vinegar.