KR102419918B1 - Microbial deodorant and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a microbial deodorant and a manufacturing method thereof and, more particularly, to a microbial deodorant with excellent deodorizing performance against harmful substances such as ammonia, trimethylamine, hydrogen sulfide, and methyl mercaptan by supplying oxygen to activate the dominant spawn of microorganisms while irradiating the same and drying the same with hot air with an oxygen supplying and exhausting device, and to a manufacturing method thereof. A liquid microbial deodorant according to the present invention has a total number of microorganisms of 1.0 X 10^9 or more and has excellent deodorizing performance against more than 90% of ammonia and trimethylamine and more than 5% of hydrogen sulfide and methyl mercaptan. A powdered microbial deodorant has a total number of microorganisms of 1.0 X 10^9 or more and has excellent deodorizing performance against more than 60% of ammonia and trimethylamine and more than 6% of hydrogen sulfide and methyl mercaptan.

Description

Microbial deodorant and manufacturing method thereof

The present invention relates to a microbial deodorant and a method for producing the same, and more particularly, by activating a microbial-dominant spawn by hot air drying with light irradiation and oxygen supplying/exhaling device and supplying oxygen, such as ammonia, trimethylamine, hydrogen sulfide, and methylmercaptan. It relates to a microbial deodorant excellent in deodorizing performance against harmful substances and a method for manufacturing the same.

The domestic livestock industry has achieved rapid quantitative growth and qualitative development, but environmental pollution such as odor and soil and water quality due to the continuous increase of livestock manure generated in this process is emerging as a social problem. Since the Enactment of the Odor Prevention Act in 2005, the number of complaints related to odors has steadily increased, about doubling from 13,103 cases in 2013 to 24,748 cases in 2016. Complaints related to odor

In general, malodor is defined as an odor in which hydrogen sulfide, mercaptans, amines, and other irritating gaseous substances stimulate the human sense of smell and cause discomfort and disgust. It is defined as an odor that causes discomfort and disgust by stimulating human sense of smell by working together with more than one odorous substance. Disulfide, trimethylamine, acetaldehyde, styrene, propionaldehyde, butyr aldehyde, n-valderaldehyde, n-valderaldehyde, I-valderaldehyde, toluene, xylene, methyl ethyl ketone, methyl isobutyr ketone, It refers to propionic acid, n-butyric acid, n-valeric acid, i-butyric acid, and i-butyr alcohol.

Meanwhile, various methods for removing odorous substances or reducing odor concentration have been proposed. Commercially available technology to remove odor substances can be divided into adsorption method, decomposition method, and microbial method. It can be divided into chemical adsorption method.

In addition, decomposition methods include ozone oxidation using ozone or UV lamps, catalytic oxidation using catalyst-supported porous ceramics or plasma, ozone catalytic oxidation combining ozone generation and catalytic oxidation, combustion using direct combustion and catalytic combustion, and decomposition of odors on the carrier. Technologies such as the microbial method, which removes odorous substances from the metabolic process of microorganisms during the process of supporting microorganisms that contain microbes and passing polluted air containing odor substances between the carriers on which the microorganisms are supported, are being applied to the removal of odor substances. This is the method of constructing this complex and large-scale facility.

As another method of removing odorous substances or reducing odor concentration, various deodorizing compositions such as transition metals capable of removing odorous substances from water, natural deodorants such as Schisandra extract, or addition of catalyst substances have been disclosed.

Currently, more than 90% of livestock manure is treated as fertilizer, and most of it is composted as solid matter. In addition to the high incidence and long maturation period, undecomposed solids and pathogenic microorganisms still remain a major problem.

In particular, unlike cattle (water content 60%), pig manure is raised in a different breeding form and has a high moisture content (more than 90%), so much more energy is required to dry moisture, and odors are generated during drying. cause serious safety problems.

In this regard, Korean Patent Application Laid-Open No. 2003-0014052 discloses a natural deodorant composition comprising a microbial group culture solution composed of Bacillus genus strains having excellent organic matter decomposition ability and forming endospores, wood vinegar extracted from natural plants, and a small amount of spices and purified water. By compensating for the shortcomings of chemical deodorants or single natural deodorants, it has a continuous and effective deodorizing ability, and at the same time has excellent antibacterial action against decay and pathogenic bacteria.

U.S. Patent No. 4996055 discloses a deodorant composition composed of butyric acid bacteria and Bacillus subtilis, which are microbial groups that do not use chemical materials, and discloses a deodorant composition using Bacillus Miyari as butyric acid bacteria and natto bacteria and Bacillus subtilis as the genus Bacillus subtilis. .

However, since these materials have low deodorization efficiency, it is difficult to treat odors generated at high concentrations in the industrial field, and various pre-treatment or post-treatment devices such as scrubbers or adsorption towers are required, and there is a problem that a large-scale treatment device is required.

Korean Patent Publication No. 2003-0014052 US Patent No. 4996055

The object of the present invention was invented to improve the above problems, and by activating the microorganism-dominant spawn by hot air drying with light irradiation and oxygen supply intake/exhaust device and supplying oxygen, such as ammonia, trimethylamine, hydrogen sulfide, methyl mercaptan, etc. An object of the present invention is to provide a microbial deodorant with excellent deodorizing performance against harmful substances and a method for manufacturing the same.

In order to achieve the above object, the present invention provides a microbial deodorant and a method for producing the same.

The method for producing the microbial deodorant is

A first step of preparing a mixture by mixing purified water, microbial dominant spawn, DTPA chelating agent, soipeptone, sodium hydroxide, amino acid, molasses, sodium chloride, dextrose monohydrate and sodium chlorite in a mixer;

a second step of irradiating light to the mixture of the first step with a top panel LED rail lighting;

a third step of supplying oxygen to the mixture irradiated with light in the second step by indirect hot air drying with an oxygen supply intake/exhaust device;

and a fourth step of subdividing and packaging the resulting mixture after the third step.

80 to 90 parts by weight of purified water in the mixture of the first step, 1 to 2 parts by weight of microbial dominant spawn, 1 to 5 parts by weight of DTPA chelating agent (culture and growth), 1 to 5 parts by weight of Soy peptone, sodium hydroxide (Sodium hydroxide) 1 to 5 parts by weight, amino acid 1 to 2 parts by weight, molasses 2 to 5 parts by weight, sodium chloride (Sodium chloride) 1 to 3 parts by weight, and glucose monohydrate (Dextrose monohydrate) 2 to 5 parts by weight It is preferred that .

The dominant microorganisms in the first stage are Rhodobacter, Bacillus subtilis and Lactobacillus pullantinum.

The Rhodobacter is Rhodobacter blasticus, Rhodobacter capsulatus, and Rhodobacter sphaeroides.

The mixing ratio of the Rhodobacter plasticus, Rhodobacter capsulatus and Rhodobacter spheroids is preferably 3-5:2-4:1-3 by weight.

In the first step, Saccharomyces cerevisiae may be further included.

In the second step, it is preferable that the wavelength of the upper panel LED rail lighting is 100 nm to 350 nm, and the intensity of the LED light source is 5 mW/cm 2 to 45 mW/cm 2 .

In the third step, the oxygen supply intake/exhaust device includes a cylindrical pipe for injecting a gas made of oxygen (O2) to the mixture of the first step, the cylindrical pipe having a body portion and a lower end, the end of the lower end is sealed, and the diameter of the cross-section of the lower end decreases toward the end of the lower end,

A plurality of first holes are provided on a side surface of the main body, and a plurality of second holes are provided on a side surface of the lower end, and the plurality of first holes and a plurality of second holes are formed through a cylindrical pipe, and oxygen It is preferable that the gas consisting of is injected from the inside of the cylindrical pipe to the outside.

In addition, there is provided a microbial deodorant prepared by the above manufacturing method.

According to the method for producing a microbial deodorant of the present invention, the dominant microorganisms such as Rhodobacter, Bacillus subtilis and Lactobacillus pullantinum are irradiated with light, and oxygen is supplied using an oxygen supply intake/exhaust device, whereby the dominant microorganisms are sufficiently fermented and It is activated and has the effect of improving the deodorization performance.

In addition, the liquid microbial deodorant has a total number of microorganisms of 1.0 X 10 ⁹ or more, 90% or more of ammonia, trimethylamine, 5% or more of hydrogen sulfide, and methyl mercaptan. X 10 ⁹ or more, ammonia, 60% or more of trimethylamine, hydrogen sulfide, 6% or more of methyl mercaptan has an effect of having excellent deodorization performance.

In addition, according to the method for producing a microbial deodorant of the present invention, the microbial dominant spawn is fermented and activated at high speed by light irradiation and oxygen supply, so that the microbial deodorant can be produced in a short period of 3 to 6 days.

1 is a process diagram of a method for producing a microbial deodorant of the present invention.
Figure 2 is a photograph of the microbial fermenter of the present invention.
Figure 3 is a photograph of the top panel LED rail lighting of the present invention.
4 is a photograph of the oxygen supply intake/exhaust device of the present invention.
5 is a photograph of a device for packaging and removing foreign substances of the present invention.
6 to 13 is a deodorizing performance test result table of the microbial deodorant prepared in Examples 1 and 2 of the present invention.
14 and 15 are explanatory pictures of the PAHs test method, As, Cd, Cr, Pb, Cu, Ni, Zn test method, Hg test method, VACs, and chlorinated hydrocarbon test method of Evaluation Examples 1 to 4 of the present invention to be.

Hereinafter, the present invention will be described in detail. In describing the present invention, detailed descriptions of related known configurations or functions may be omitted.

The terms or words used in the present specification and claims are not limited and interpreted in a conventional or dictionary meaning, but should be interpreted as meanings and concepts consistent with the technical matters of the present invention.

The embodiments described in this specification and the configurations shown in the drawings are preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention, so various equivalents and modifications that can replace them at the time of the present application there may be

The method for producing the microbial deodorant is

A first step of preparing a mixture by mixing purified water, microbial dominant spawn, DTPA chelating agent, soipeptone, sodium hydroxide, amino acid, molasses, sodium chloride, dextrose monohydrate and sodium chlorite in a mixer;

a second step of irradiating light to the mixture of the first step with a top panel LED rail lighting;

a third step of supplying oxygen to the mixture irradiated with light in the second step by indirect hot air drying with an oxygen supply intake/exhaust device;

and a fourth step of subdividing and packaging the resulting mixture after the third step.

80 to 90 parts by weight of purified water in the mixture of the first step, 1 to 2 parts by weight of microbial dominant spawn, 1 to 5 parts by weight of DTPA chelating agent (culture and growth), 1 to 5 parts by weight of Soy peptone, sodium hydroxide (Sodium hydroxide) 1 to 5 parts by weight, amino acid 1 to 2 parts by weight, molasses 2 to 5 parts by weight, sodium chloride (Sodium chloride) 1 to 3 parts by weight, and glucose monohydrate (Dextrose monohydrate) 2 to 5 parts by weight It is preferred that .

Purified water may be used as the diluted solution of the present invention, and 80 to 90 parts by weight of purified water may be used, and more preferably 80 to 85 parts by weight may be used to dilute the mixture.

In the present invention, a microbial dominant spawn may be used, and 1 to 2 parts by weight of the microbial dominant spawn may be used. The dominant microorganisms in the first stage are Rhodobacter, Bacillus subtilis and Lactobacillus pullantinum.

The Rhodobacter is Rhodobacter blasticus, Rhodobacter capsulatus, and Rhodobacter sphaeroides. Bacteria of the genus Rhodobacter are facultative aerobic bacteria isolated from seawater freshwater and soil environments. It is a Gram-negative bacterium and divides cells by double division or budding. It changes from yellow-green to yellow when cultured under photosynthetic conditions, and from pink to red in aerobic culture.

In the present invention, useful microorganisms are cultured using Rhodobacter plasticus, Rhodobacter capsulatus, and Rhodobacter spheroids of the genus Rhodobacter. The mixing ratio of the Rhodobacter plasticus, Rhodobacter capsulatus and Rhodobacter spheroids is preferably 3-5:2-4:1-3 by weight.

In general, Rhodobacter plasticus, Rhodobacter capsulatus and Rhodobacter spheroides grow well at 27°C to 30°C, neutral pH 6 to 8, and at a neutral pH, the above Rhodobacter plaque The mixture of 3~5:2~4:1~3 weight ratio of Styx, Rhodobacter capsulatus and Rhodobacter spheroides achieves the most ideal growth, has the fastest growth and culture rate, and improves deodorization performance. can do it

Among the microorganisms dominant in the first stage, Bacillus subtilis is a bacterium of the genus Bacillus and is widely distributed in the surrounding environment such as soil, human body, animals, and the like. Bacillus subtilis of the present invention has important enzyme activities such as amylase and protase, and can exhibit deodorizing properties. Bacillus subtilis of the present invention, often referred to as 'Bacillus subtilis', is a spore-forming bacterium that mainly inhabits in soil and to which the genus Bacillus belongs, and has a positive reaction in a Gram reaction, and is aerobic and main Exercise with maternal flagella. Spores oval, centrally located. It is closely related as a fermenting microorganism of cheonggukjang and is also used as a preparation for various microorganisms. In addition, it is very importantly used as a plasmid of recombinant DNA technology in genetic engineering.

Among the microorganisms dominant in the first stage, Lactobacillus fulantinum is a bacterium of the genus Lactobacillus that ferments sugars to obtain energy and produces a large amount of lactic acid. There are single, double-stranded, single-chain, and single-chain types of bacterial arrangement, and it is useful for reducing odor and reducing sludge by generating lactic acid from various sugars.

A chelate refers to a complex ion formed by coordinating one ligand with a metal ion at two or more sites. For example, when an aqueous solution of ethylenediamine is added to a solution containing copper, copper bonds with nitrogen atoms of two amine groups of ethylenediamine to form a cyclic complex ion, and the resulting compound is called a chelate.

The chelate functions to make the nutrients necessary for crops in a good state for absorption. At this time, EDTA (Ethylenediamine tetraacetic acid) and DTPA (Diethylenetriamine pentaacetic acid) are representative synthetic chelating agents.

The present invention may use a DTPA chelating agent (culture and growth), and preferably contains 1 to 5 parts by weight. By using the DPTA chelating agent, nutrients can be absorbed well, and the dominant microorganism can be cultured to further improve performance. In addition, if a chelating agent is sprayed on fixed or insolubilized nutrients such as nitrogen, phosphoric acid, potassium, calcium, and magnesium among the components of the microbial deodorant, potassium, calcium, trace elements, and insolubilized phosphoric acid are separated from the bad odor for easy absorption by microorganisms. There are advantages to helping.

The present invention can use soy peptone (Soy peptone), it is preferable to include 1 to 5 parts by weight. Soy peptone is an enzymatically digested soy protein, which contains a high ratio of low molecular weight peptides, free amino acids and growth promoters, so it can be used as an excellent medium for the growth of various microorganisms in the fermentation industry.

Soy peptone is an enzymatically digested soy protein, which contains a high ratio of low molecular weight peptides, free amino acids and growth promoters.

Unlike raw materials such as animal-derived extracts, it is 100% plant-derived, so it is safe from infectious diseases such as mad cow disease and avian influenza. Soy peptone contains a variety of amino acids such as free amino acids, and provides a rich source of nutrients for the growth of microorganisms nutritionally.

In addition, Lactobacillus, Bifidobacterium sp., which are widely used in the dairy industry, lactic acid bacteria, Bacillus sp., E. coli, Yeast & mold It also exhibits high performance in detection.

In the present invention, sodium hydroxide may be used, and it is preferable to include 1 to 5 parts by weight. Sodium hydroxide is used as an acidity regulator when culturing microorganisms, and by using it, microbial production efficiency is increased.

The present invention may use an amino acid, preferably including 1 to 2 parts by weight, and containing the amino acid to help the decomposition of the enzyme through the acid / base reaction can improve the deodorizing component.

The present invention may use sodium chloride (Sodium chloride), preferably containing 1 to 3 parts by weight, sodium chloride (Sodium chloride) can be dissolved in purified water as white crystals, it can be used as a fermentation aid.

In the present invention, molasses can be used, and it is preferable to include 2 to 5 parts by weight, and molasses is a by-product that is no longer crystallized and remains in the process of processing sugar cane or sugar beet into sugar as a fermentation aid. It has a dense syrup form.

In the present invention, Dextrose monohydrate can be used, and it is preferable to include 2 to 5 parts by weight. Dextrose monohydrate is used to facilitate fermentation during LED irradiation. can

In the first step, Saccharomyces cerevisiae may be further included. By further including the Saccharomyces cerevise, it can help the rapid growth of microorganisms.

The Saccharomyces cerevisiae is a natural live yeast, and unlike artificial artificial yeast that is generally cultured using chemical substances, various microorganisms act when the natural live yeast is fermented. At this time, the amount of protein decomposition into amino acids is It has the advantage of being soft to the touch, and has the advantage of helping the culture performance of the dominant spawn through continuous fermentation for a long time.

In the second step, it is preferable that the wavelength of the upper panel LED rail lighting is 100 nm to 350 nm, and the intensity of the LED light source is 5 mW/cm 2 to 45 mW/cm 2 .

A light source is required for the fermentation reaction of the microbial dominant spawn of the present invention. These light sources must be low cost, durable and highly efficient for commercial production. One of the light sources that satisfy these conditions is a light-emitting diode (LED). Because LED is small and light, it can be directly illuminated for any type of micro-aeration reaction, has a long average lifespan, and has high electrical efficiency, making it suitable as a light source for fermentation reactions of dominant microorganisms. If the LED light source emits light in a specific wavelength region and this wavelength region overlaps with the action spectrum of photosynthesis, it is an advantage to increase the efficiency of the supplied light energy. However, the conventional LED cannot be used as a light source for the fermentation reaction of the dominant microorganism due to the small amount of light. It can be used to grow microorganisms.

The LED light source may be irradiated with light using a plurality of LED lamps. In addition, the light irradiation by the LED light source in the second step can be irradiated with light in a non-contact state with the mixture of the first step from a plurality of LED lamps installed on the high-speed fermentation equipment. In addition, light can be irradiated in contact with the mixture of the first step in a plurality of LED lamps installed on the floor inside the high-speed fermentation equipment. In this way, there is an advantage in that the light irradiation efficiency can be increased by performing LED light irradiation on the upper part of the mixture and the inside of the mixture.

In the present invention, the LED wavelength region can use the ultraviolet region, and when it is out of the above range, the culture conditions deteriorate and the number of microorganisms decreases. For example, in the visible or infrared region, there is a risk of a decrease in the number of microorganisms due to light irradiation, which is not preferable.

The intensity of the light source of the present invention may be 5mW/cm2 to 45mW/cm2, and if it is less than the above range, it is difficult to expect an effect on light irradiation, and if it exceeds the above range, the number of microorganisms is rather reduced, which is not preferable.

Indirect hot air drying with the oxygen supply intake and exhaust device of the present invention may include a third step of supplying oxygen. The oxygen supply intake/exhaust device may inject oxygen by inserting a cylindrical pipe into the mixture to maintain a constant oxygen concentration in the mixture and activate the activity of aerobic microorganisms.

In the third step, the oxygen supply intake/exhaust device includes a cylindrical pipe for injecting a gas consisting of oxygen (O ₂ ) to the mixture of the first step, the cylindrical pipe having a main body and a lower end, the end of the lower end is sealed, and the diameter of the cross section of the lower end decreases toward the end of the lower end,

A plurality of first holes are provided on a side surface of the main body, and a plurality of second holes are provided on a side surface of the lower end, and the plurality of first holes and a plurality of second holes are formed through a cylindrical pipe, and oxygen It is preferable that the gas consisting of is injected from the inside of the cylindrical pipe to the outside.

The oxygen supply intake/exhaust device of the present invention may include a cylindrical pipe. Oxygen is supplied through an inlet of the cylindrical pipe to inject oxygen into the mixture through a plurality of first holes and a plurality of second holes. Although it is possible to inject air, it is preferable to inject a gas made of oxygen in order to maintain a high oxygen concentration.

The cylindrical pipe of the present invention has a main body and a lower end, the end of the lower end is sealed, and when the direction perpendicular to the ground of the cylindrical pipe is defined as the z-axis, the xy-plane of the lower end toward the end of the lower end The diameter of the cross-section of the phase may be reduced. In this way, the cylindrical pipe of the oxygen supply intake and exhaust device can be easily inserted into the mixture.

In addition, a plurality of first holes are provided on a side surface of the main body portion and a plurality of second holes are provided on a side surface of the lower end portion, so that oxygen in the cylindrical pipe can be finely sprayed to the outside. In particular, oxygen bubbles may be supplied to the side surface of the cylindrical pipe by a plurality of first holes on the side surface of the body part, and oxygen may be supplied radially to the lower surface of the cylindrical pipe by a plurality of second holes on the side surface of the lower end part. can

The number of operations for injecting the gas made of oxygen in the oxygen supply intake and exhaust device of the present invention is 2 to 3 times / min, to 3 to 4 times / min, and the one operation time is 30 to 40 seconds Adjustment timer setting condition value. It is preferable to be driven. That is, when the number and time of the operation of spraying the gas made of oxygen exceeds the above range, excess oxygen is injected and the activity of aerobic microorganisms is rather disturbed, and the efficiency may be lowered than that of not injecting oxygen.

The present invention may include a fourth step of subdividing and packaging the resulting mixture after the third step. The second step and the third step of the present invention are performed simultaneously, and the second step and the third step may be performed for 3 to 5 days, more preferably 3 to 4 days.

As such, it has the advantage of being able to produce a microbial deodorant in a short period of 3 to 6 days by reducing the aging time required for more than one week by the light irradiation of the second stage and the oxygen supply by the oxygen supply intake/exhaust device of the third stage. .

In addition, there is provided a microbial deodorant prepared by the above manufacturing method. The microbial deodorant prepared by the manufacturing method of the present invention can be used as a deodorant in liquid or powder form.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it is obvious to those of ordinary skill in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention. .

Example 1 Preparation of liquid microbial deodorant

Purified water 84g, Rhodobacter plastikus 1g, Rhodobacter capsulatus 0.6g, Rhodobacter spheroids 0.4g, Bacillus subtilis 2g, Lactobacillus plantinum 1g, DPTA chelating agent 3g, Soipeptone 3g, Sodium hydroxide 3g , amino acid 2g, molasses 3g, sodium chloride 2g and glucose monohydrate 3g were mixed, exposed to an LED light source with a wavelength of 225 nm and an intensity of 15 mW/cm 2 , and fine holes were drilled 3 times per minute at 30 second intervals using an oxygen supply intake and exhaust device. A liquid microbial deodorant was prepared by supplying oxygen to a cylindrical pipe having and aging for 3 days.

Example 2 Preparation of powdered microbial deodorant

Purified water 85g, Rhodobacter plastikus 1g, Rhodobacter capsulatus 1g, Rhodobacter spheroides 1g, Bacillus subtilis 2g, Lactobacillus plantinum 1g, DPTA chelating agent 1g, Soipeptone 1g, Sodium hydroxide 1g, Amino acid 2 g, molasses 3 g, sodium chloride 1 g, and glucose monobasic salt were mixed, exposed to an LED light source with a wavelength of 225 nm and an intensity of 15 mW/cm 2 , and a cylindrical shape having micro-holes 3 times per minute at an interval of 30 seconds using an oxygen supply intake and exhaust device A powdery microbial deodorant was prepared by supplying oxygen to the pipe, aging for 3 days, and aging and drying for 24 hours.

Evaluation Example 1 Confirmation of the total number of aerobic microorganisms

The total number of aerobic microorganisms was confirmed by requesting the microbial deodorant prepared in Examples 1 and 2 to the Korea Convergence Chemical Testing Institute, respectively. 6, the total number of aerobic microorganisms of the liquid microbial deodorant prepared in Example 1 is 1.6x109 CFU/mL, and referring to FIG. 7, the total number of aerobic microorganisms in the powdered microbial deodorant prepared in Example 2 is 1.1x109 CFU/mL.

Therefore, it was confirmed that microorganisms can be effectively cultured within a short period of time, and it was confirmed that the microbial growth and development promotion effect was excellent.

Evaluation Example 2 Deodorization Performance Test 1

In order to confirm the deodorizing performance of the microbial deodorant prepared in Examples 1 and 2, each was requested by the Korea Research Institute of Convergence Chemicals and VACs, benzene, toluene, xylene, ethylbenzene, 1,4-dichlorobenzene, styrene, The detected amounts of PAHs, Acenaphthene, Acenaphthylene, Anthracene, and Benzo(a)anthracene were confirmed.

8, in the liquid microorganism deodorant prepared in Example 1, VACs, benzene, toluene, xylene, ethylbenzene, 1,4-dichlorobenzene, styrene, PAHs, Acenaphthene, Acenaphthylene, Anthracene, Benzo (a) It can be confirmed that anthracene is not detected.

In addition, referring to FIG. 11, in the powdered microbial deodorant prepared in Example 2, VACs, benzene, toluene, xylene, ethylbenzene, 1,4-dichlorobenzene, styrene, PAHs, Acenaphthene, Acenaphthylene, Anthracene, Benzo (a) It can be confirmed that anthracene is not detected.

Evaluation Example 3 Deodorization Performance Test 2

In order to confirm the deodorizing performance of the microbial deodorant prepared in Examples 1 and 2, benzo(a)pyrene, benzo(b)fluoranthene, Benzo(g,h,i)perylene, Detected amounts of benzo(k)fluorathene, Chrysene, Dibenzo(a,h)anthracene, fluoranthene, fluorene, indeno(1,2,3-cd)pyrene, naphthalene, phenanthrene, and pyrene were confirmed.

Referring to FIG. 9, in the liquid microorganism deodorant prepared in Example 1, benzo(a)pyrene, benzo(b)fluoranthene, Benzo(g,h,i)perylene, Benzo(k)fluorathene, Chrysene, Dibenzo(a, h) It can be confirmed that anthracene, fluoranthene, fluorene, indeno(1,2,3-cd)pyrene, naphthalene, phenanthrene, and pyrene are not detected.

In addition, referring to FIG. 12, in the powder-type microorganism deodorant prepared in Example 2, benzo(a)pyrene, benzo(b)fluoranthene, Benzo(g,h,i)perylene, Benzo(k)fluorathene, Chrysene, Dibenzo It can be seen that (a,h)anthracene, fluoranthene, fluorene, indeno(1,2,3-cd)pyrene, naphthalene, phenanthrene, and pyrene are not detected.

Evaluation Example 4 Deodorization Performance Test 3

In order to confirm the deodorizing performance of the microbial deodorant prepared in Examples 1 and 2, the chlorinated hydrocarbons, dichloromethane, chloroform, carbon tetrachloride, 1,1,1-trichloroethane, The detected amounts of 1,1-dichloroethylene, trichloroethylene, tetrachloroethylene, As, Cd, Cr, Pb, Cu, Ni, Zn, and Hg were confirmed.

Referring to FIG. 10, in the liquid microorganism deodorant prepared in Example 1, chlorine-based hydrocarbons, dichloromethane, chloroform, carbon tetrachloride, 1,1,1-trichloroethane, 1,1-dichloroethylene, trichloroethylene, tetra It can be seen that chloroethylene, As, Cd, Cr, and Pb are not detected, Cu is 0.13 mg/kg, Ni is 0.07 mg/kg, and referring to FIG. 6 , Zn is 0.7 mg/kg and Hg is not detected It can be seen that the deodorizing ability is superior to that of the conventional microbial deodorant.

In addition, referring to FIG. 13, in the powdered microbial deodorant prepared in Example 2, chlorine-based hydrocarbons, dichloromethane, chloroform, carbon tetrachloride, 1,1,1-trichloroethane, 1,1-dichloroethylene, trichloro It can be seen that ethylene, tetrachloroethylene, As, Cd, Cr, and Pb are not detected, Cu is 0.25 mg/kg, Ni is 0.21 mg/kg, and referring to FIG. 7 , Zn is 2.38 mg/kg, Hg is It can be confirmed that it is not detected, so it can be seen that the deodorizing ability is superior to that of the conventional microbial deodorant.

Evaluation Example 5 Deodorization Performance Test 4

In order to confirm the deodorizing performance of the microbial deodorant prepared in Examples 1 and 2, the amounts of ammonia, trimethylamine, hydrogen sulfide, and methyl mercaptan were detected by requesting the Korea Convergence Chemistry Research Institute, respectively.

6, it was confirmed that the liquid microbial deodorant prepared in Example 1 deodorized 90.4% of ammonia, 96.2% of trimethylamine, 6.0% of hydrogen sulfide, and 6.3% of methylmercaptan. In addition, referring to FIG. 7 , it was confirmed that 68.8% of ammonia, 72.5% of trimethylamine, 10.0% of hydrogen sulfide, and 6.3% of methyl mercaptan of the powdery microbial deodorant prepared in Example 2 were deodorized.

Claims (9)

80 to 85 parts by weight of purified water, 1 to 2 parts by weight of the dominant microorganism, 1 to 5 parts by weight of DTPA chelating agent (culture and growth), 1 to 5 parts by weight of soipeptone, 1 to 5 parts by weight of sodium hydroxide, 1 to 2 parts by weight of amino acids A first step of preparing a mixture by mixing parts by weight, 2 to 5 parts by weight of molasses, 1 to 3 parts by weight of sodium chloride, and 2 to 5 parts by weight of glucose monohydrate in a mixer;
a second step of irradiating light to the mixture of the first step with a top panel LED rail lighting;
a third step of supplying oxygen to the mixture irradiated with light in the second step by indirect hot air drying with an oxygen supply intake/exhaust device;
A fourth step of subdividing and packaging the mixture produced after the third step;
here
The dominant microorganisms of the first stage are Rhodobacter blasticus, Rodobacter capsulatus, Rodobacter sphaeroides, Bacillus subtilis and Lactobacillus pullantinum,
The Rhodobacter plasticus, Rhodobacter capsulatus, Rhodobacter spheroides, Bacillus subtilis and Lactobacillus pullantinum are each mixed in a weight ratio of 1: 0.6 to 1: 0.4 to 1: 2: 1,
Further comprising Saccharomyces cerevisiae in the first step,
In the second step, the wavelength of the upper panel LED rail lighting is 100 nm to 350 nm, and the intensity of the LED light source is 5mW/cm2 to 45mW/cm2,
In the third step, the oxygen supply intake/exhaust device includes a cylindrical pipe for injecting a gas consisting of oxygen (O ₂ ) to the mixture of the first step, the cylindrical pipe having a main body and a lower end, the end of the lower end is sealed, and the diameter of the cross section of the lower end decreases toward the end of the lower end,
A plurality of first holes are provided on a side surface of the body portion, and a plurality of second holes are provided on a side surface of the lower end portion, and the plurality of first holes and a plurality of second holes are formed through a cylindrical pipe, and oxygen A method for producing a microbial deodorant, characterized in that the gas is sprayed from the inside of the cylindrical pipe to the outside. delete delete delete delete delete delete delete delete

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