KR102186233B1 - Composition for removing odor from livestock manure comprising humic substance and lactic acid bacteria - Google Patents

Abstract

The present invention relates to a composition for removing odor from livestock manure containing a humic substance and lactic acid bacteria, and as a physiologically active substance derived from a natural material, peat moss, as a physiologically active substance, Lactobacillus plantarum ( Lactobacillus plantarum ) or Streptococcus thermophilus , in particular, by confirming that a composition containing Streptococcus thermophilus reduces ammonia and mercaptomethanol, which are malodorous components of livestock manure, the corrosive substances and strepto of the present invention It is possible to develop a product for reducing odor of livestock manure made of eco-friendly natural materials by using a composition for removing odor from livestock manure including caucus thermophilus, and is expected to help improve the breeding environment of livestock.

Description

Composition for removing odor from livestock manure comprising humic substance and lactic acid bacteria}

The present invention relates to a composition for removing odor from livestock manure containing a humic substance and lactic acid bacteria.

The tendency of livestock breeding to become denser and enclosed is causing air pollution problems, especially odor complaints that are frequently raised around farms. The number of complaints about livestock odor is increasing every year to 4,300 in 2015 and 6,400 in 2016, and the Republic of Korea has designated the target of odor management in a specific'facility' in the'Odor Prevention Act' As'regions' and strengthening the standards and management of odor emission, livestock farms are urgently required to maintain the sustainability and productivity of the pig farming industry, while maintaining profitability and simple odor reduction efforts and the development of eco-friendly livestock manure treatment technology ( Eunyoung Lee, et al., 2010).

The odor and harmful gas from the manure waste from the barn acts as a major pollutant that damages rivers, land, air and crops, and also serves as a means of spreading livestock infectious diseases and parasitic diseases.

The main components of livestock odor include ammonia, sulfur compounds, indole, and phenols, and are produced by anaerobic fermentation of manure when storing or processing livestock's digestive system or livestock manure. These livestock odor components adversely affect human and livestock health. In humans, it causes mild inflammation, sneezing, and drooling, and when it reaches severe levels, it can cause immediate death. Likewise for livestock, productivity is greatly reduced, such as inhibiting growth and causing diseases of the respiratory system in closed sheds.

Furthermore, it is causing a lot of damage to nearby farmhouses and communities around livestock stalls. The odor and harmful gases released from the inside of the barn pollute the pleasant atmosphere, and when reaching land and water through rain, it may lead to acidification of the soil and eutrophication of rivers (Dongyoon Choi, et al., 2009).

Currently, there are about 300-400 types of odor reduction (removal) agents for livestock that are in circulation, and their names are also used interchangeably as odor inhibitors and livestock environment improvement agents. Odor reducing agents for livestock are largely divided into four depending on the method of action.

1) Microbial preparations such as lactic acid bacteria and enzymes are directly involved in the production and maintenance of normal microflora in the intestine of livestock, promoting the proliferation of useful microorganisms with high metabolic activity, improving digestibility, and increasing feed efficiency to reduce odor. It reduces the concentration of organic matter in the manure, which is known as a major cause of occurrence. Separately, in the process of proliferation of photosynthetic bacteria by directly spraying microbial agents such as photosynthetic bacteria on livestock manure, odor sources such as volatile lower fatty acids and hydrogen sulfide are used as nutrients to fundamentally remove odors.

2) Natural and herbal extract formulations not only can supplement the toxicity and persistence problems of artificial and synthetic products, but are also gaining popularity recently because of their customary safety recognition, promoting the secretion of digestive enzymes in the intestine of livestock and barriers. It is known to contain a large amount of unknown growth factor (UGF) that is absorbed into the intestine, which increases the vitality of intestinal microorganisms and livestock, and contributes to the improvement of digestibility by selecting and adjusting harmful and beneficial bacteria.

3) Mineral preparations such as zinc and copper maximize digestion efficiency by adsorbing harmful ingredients or substances that cause odor in the intestines of livestock and acting as a prosthetic group of digestive enzymes. Classically, activated carbon has a high surface adsorption property, which directly adsorbs odorous substances such as ammonia gas, contributing to odor suppression.

4) The odor reducing agent, which uses disinfectants and pesticides as active ingredients, kills harmful microorganisms such as spoilage by spraying or spraying directly on sources such as livestock and manure, and harms such as flies, mosquitoes, and cockroaches that are considered as propagating media of harmful microorganisms By removing pests and harmful rodents, the effect of odor removal is expected.

However, it is a very difficult problem to remove the odor that has already occurred inside the barn so that it does not affect livestock. In order to solve this problem, many studies have been conducted in various fields, but a satisfactory solution has not been found, and a method of removing odors from livestock facilities is currently being studied from various viewpoints.

Accordingly, the present inventors, in the process of developing a composition for removing odor from livestock manure made of an eco-friendly material, a composition containing a physiologically active substance derived from a natural material, peat moss, and lactic acid bacteria, ammonia, a odor component, The present invention could be completed by confirming that hydrogen sulfide and mercaptomethanol are significantly reduced.

Korean Patent Publication No. 2003-0000028, which is a prior art, describes a feed additive in which microorganisms and enzymes are mixed with a corrosive substance having a high content of fulvic acid and the effect of reducing odor in manure due to its intestinal bacteriostatic action. The composition is different from the composition for removing malodor, which reduces malodor components by spraying inside the house and on the manure slurry. In addition, Korean Patent Application Publication No. 2012-0104507 describes that it is possible to remove the odor of manure by using fertile soil or humus, which contains various soil microorganisms such as lactic acid bacteria, yeast bacteria, and Bacillus bacteria. As a method of separating a odor-removing seed from water and a composting method using such a seed to remove odor, the combination of the lactic acid bacteria and corrosive substances of the present invention and the resulting increased odor removal effect are not described.

Eunyoung Lee, et al., (2010), which is a non-patent document, describes the effect of removing major odor substances from pig manure through the addition of a microbial agent, but the increased odor removal effect by the combination of corrosive substances and lactic acid bacteria of the present invention is described. Not.

Korean Patent Laid-Open Publication No. 2003-0000028, Feed additive manufacturing and use method, 2003. 01. 03. Publication. Korean Patent Publication No. 2012-0104507, Method for producing and composting seeds for odor removal using livestock manure, 2012. 09. 21. Published.

Eun-Young Lee, et al., Current Situation and Prospect of Using Environmental Improvement Agents to Solve Odor Characteristics and Problems in Pig Manure, Kor. J. Microbiol., Biotechnol., 38(3), 244-254, 2010. Dongyoon Choi, et al., Efficiency of removing pollutants and odors from pig manure slurry using filter material circulation system, livestock facility environment, 15(3), 217-224, 2009.

It is an object of the present invention to provide a composition for removing odor from livestock manure containing corrosive substances and lactic acid bacteria.

The present invention relates to a composition for removing odor from livestock manure containing a humic substance and lactic acid bacteria.

The corrosive material may contain 0.08 to 0.4% by weight based on the total weight of the composition.

The lactic acid bacteria may contain 1×10 ⁸ ~ 5×10 ⁹ CFU/ml based on the total volume of the composition.

The lactic acid bacteria may be Lactobacillus plantarum , Streptococcus thermophilus , or a mixture thereof. Preferably, it is Streptococcus thermophilus.

The composition may be sprayed inside the barn or on the manure slurry.

Hereinafter, the present invention will be described in detail.

The present invention relates to a composition for removing odor from livestock manure containing corrosive substances and lactic acid bacteria.

In the present invention, "humic substance" means all organic matter present in the soil other than living organisms. The corrosive material of the present invention is a corrosive material derived from peat moss (aquatic plants and other corrosives accumulated in peat soil, wetlands, swamps, etc.).

The corrosive substance is a natural physiologically active substance extracted using natural material peat moss. It increases the vigorous proliferation and activity of beneficial microorganisms, suppresses the occurrence of odor components such as ammonia and hydrogen sulfide generated by intestinal microorganisms, and has antibacterial effect. It can act to inhibit the growth of microorganisms that produce ingredients.

The corrosive material may be included in 0.05 to 1% by weight based on the total weight of the composition. It is preferably 0.05 to 0.5% by weight, more preferably 0.08 to 0.4% by weight, still more preferably 0.2 to 0.4% by weight, and most preferably 0.4% by weight. However, it is not limited thereto. When the amount of the corrosive material is less than 0.05% by weight, the effect of removing odor is lowered due to the addition of a small amount, and it is not preferable, and when it is more than 1% by weight, the cost burden on the manufacturing process increases, which is not preferable.

The lactic acid bacteria of the present invention are beneficial bacteria used in fermented products, and are active in the production of lactic acid, so they kill harmful microorganisms to suppress the generation of odor, and contribute to the suppression of odor by the flavor components of the lactic acid bacteria.

The lactic acid bacteria are Lactobacillus plantarum ( Lactobacillus plantarum ), Streptococcus thermophilus ( Streptococcus thermophilus ) or may be a mixture thereof, preferably Streptococcus thermophilus . However, it is not limited thereto.

The lactic acid bacteria may be a culture medium cultured in a lactic acid bacteria culture liquid medium or a lactic acid bacteria obtained by separately separating the lactic acid bacteria from the culture medium.

The lactic acid bacteria may contain 1×10 ⁷ ~ 1×10 ¹⁰ CFU/ml based on the total volume of the composition. It is preferably 5×10 ⁷ to 5×10 ⁹ CFU/ml, and most preferably 1×10 ⁸ to 5×10 ⁹ CFU/ml. However, it is not limited thereto. If the lactic acid bacteria is less than 1×10 ⁷ CFU/ml, it is not preferable because the function of the lactic acid bacteria decreases and it cannot kill harmful microorganisms, so it may not be able to suppress the generation of odor components.If the lactic acid bacteria are more than 1×10 ¹⁰ CFU/ml It is difficult to cultivate a high number of lactic acid bacteria, which is not preferable in the manufacturing process.

The odor of livestock manure is that when the digestive system of livestock or livestock manure is stored or processed, odor components are generated as the manure is anaerobic fermentation, and may be caused by ammonia, hydrogen sulfide and mercaptomethanol.

The livestock may be cattle, pigs, chickens, ducks, and the like.

The composition for removing odor of livestock manure may remove odor by reducing or removing odor components such as ammonia, hydrogen sulfide, and mercaptomethanol by spraying inside the livestock shed or on the manure slurry.

The present invention relates to a composition for removing odor from livestock manure containing a humic substance and lactic acid bacteria, and as a physiologically active substance derived from a natural material, peat moss, as a physiologically active substance, Lactobacillus plantarum ( It was confirmed that a composition containing Lactobacillus plantarum ) or Streptococcus thermophilus , especially Streptococcus thermophilus , reduced ammonia and mercaptomethanol, which are malodorous components of livestock manure.

Through this, by using the composition for removing odor of livestock manure containing corrosive substances and lactic acid bacteria of the present invention, it is possible to develop a product for reducing odor of livestock manure made of eco-friendly natural materials, and it is expected that it will be helpful in improving the breeding environment of livestock.

1 is a result of confirming the effect of removing ammonia (A) and mercaptomethanol (B), which are malodorous components in pig manure, according to the treatment of a composition containing a corrosive substance or lactic acid bacteria alone and a corrosive substance and lactic acid bacteria in vitro Is showing.
Figure 2 shows the results of confirming the ammonia removal effect according to the corrosion substance alone treatment in a pig farm.
Figure 3 is in the air of ammonia, which is a malodorous component, according to treatment of a composition containing Streptococcus thermophilus alone or a corrosive substance of the present invention and Streptococcus thermophilus in the inside (A) or manure slurry (B) of a pig farm. It shows the result of checking the content.

Hereinafter, a preferred embodiment of the present invention will be described in detail. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the content introduced herein becomes thorough and complete, and is provided to sufficiently convey the spirit of the present invention to those skilled in the art.

<Experimental Example 1. Confirmation of the effect of reducing odor of the composition containing corrosive substances and lactic acid bacteria>

In order to compare the odor reduction effect of the composition containing corrosive substances and lactic acid bacteria, the reduction effect of ammonia (NH ₃ ), hydrogen sulfide (H ₂ S) and mercaptomethanol (R·SH), which are the main components of each composition, was confirmed. .

As a humus material, a caustic material derived from peat moss was used, and as a microorganism, Streptococcus thermophilus and Lactobacillus plantarum were used as lactic acid bacteria.

The Streptococcus thermophilus and Lactobacillus plantarum were MRS liquid medium (1% proteose peptone No.3, 0.5% yeast extract, 1% beef extract, 2% dextrose, 0.1% polysorbate 80, 0.2% ammonium citrate, 0.5 % sodium acetate, 0.01% magnesium sulfate, 0.005% manganese sulfate, 0.2% dipotassium phosphate) were each inoculated and cultured for 48 hours at 37°C. Each culture medium contained 2.3×10 ⁹ CFU of Streptococcus thermophilus. It was confirmed that (colony forming unit)/ml, Lactobacillus plantarum contained 2.6×10 ⁹ CFU/ml.

200 ml of pig manure collected from pig farms was put into a container, treated so that the final content of humus or lactic acid bacteria corresponded to the content of each treatment group listed in Table 1 below, and then for 12, 24, and 48 hours at 40°C. After reacting, air was collected, and ammonia, hydrogen sulfide and mercaptomethanol were measured using a Gastec detector (Gastec Corporation, Japan), and the results are shown in FIG. 1. At this time, the pig manure was treated as a negative control.

Treatment group Corrosive substances
(weight%) Lactobacillus (CFU/ml) Lactobacillus Planta Room Streptococcus Thermophilus Treatment group 1 0.4 - - Treatment group 2 0.08 - - Treatment group 3 - 4.5×10 ⁸ - Treatment group 4 - 9.0×10 ⁸ - Treatment group 5 - 1.7×10 ⁹ - Treatment group 6 0.4 4.5×10 ⁸ - Treatment group 7 0.4 9.0×10 ⁸ - Treatment group 8 0.4 1.7×10 ⁹ - Treatment group 9 - - 4.5×10 ⁸ Treatment group 10 - - 9.0×10 ⁸ Treatment group 11 - - 1.7×10 ⁹ Treatment group 12 0.4 - 4.5×10 ⁸ Treatment group 13 0.4 - 9.0×10 ⁸ Treatment group 14 0.4 - 1.7×10 ⁹

As shown in the ammonia measurement result of FIG. 1(A), when Lactobacillus plantarum was treated alone (treatment group 3) or with corrosive substances (treatment group 6) so as to be 4.5×10 ⁸ CFU/ml, the ammonia level Was significantly increased compared to the negative control group, and when Lactobacillus plantarum was treated alone (treatment group 4) or with humus (treatment group 7) so that it became 9.0×10 ⁸ CFU/mL, it appeared similar to the negative control group. In the case where Lactobacillus plantarum was treated alone (treatment group 5) or with corrosive substances (treatment group 8) so as to be 1.7×10 ⁹ CFU/ml, it was confirmed that the ammonia level decreased compared to the negative control group.

On the other hand, in the case of treatment with Streptococcus thermophilus alone (treatment group 9) or with corrosive substances (treatment group 12) so as to be 4.5×10 ⁸ CFU/ml, ammonia levels increased at 12 hours compared to the negative control group, After 24 hours, it was confirmed that it was similar or lowered to that of the negative control group, and Streptococcus thermophilus alone (treatment groups 10 and 11) or with corrosive substances (treatment groups 13 and 14) so as to be more than 9.0×10 ⁸ CFU/ml. ) If the processing in the case where in comparison to the negative control ammonia levels decrease was, especially, 1.7 × 10 ⁹ CFU / ㎖ of Streptococcus Thermo filler's and (group treated with the corrosion material 14) treatment is 1.7 × 10 ⁹ CFU / Unlike the case where ml of Streptococcus thermophilus was treated alone (treatment group 11), it was confirmed that ammonia was not detected at all.

In addition, as shown in the mercaptomethanol results of FIG. 1(B), when treated with Lactobacillus plantarum alone or with a corrosive substance (treatment groups 4, 5, 7, 9), mercapto was not detected in the negative control group. While methanol levels were increased, mercaptomethanol was not detected at all as in the negative control when Streptococcus thermophilus was treated alone or with humus (treatment groups 9 to 14).

Hydrogen sulfide, another malodorous component, was not detected in all treatment groups.

Through this, when Streptococcus thermophilus is used as lactic acid bacteria, it has excellent effect of removing odor from livestock manure compared to Lactobacillus plantarum, which is another lactic acid bacteria, and corrosive substances when Streptococcus thermophilus is treated together with corrosive substances. It was confirmed that the effect of removing odor from livestock manure was increased compared to either alone or Streptococcus thermophilus alone.

< Example 1. Corrosive substances and Streptococcus Thermophilus Preparation of a composition for removing odor containing livestock manure>

Streptococcus thermophilus was cultured under the same conditions as in Experimental Example 1.

Based on the results of Experimental Example 1, the corrosive substances in tap water were mixed to be 0.4% by weight based on the total weight of the composition, and the Streptococcus thermophilus was 1.7×10 ⁹ CFU/ml to remove the odor of livestock manure of the present invention. The composition was prepared.

< Experimental example 2. Corrosive substances and Streptococcus Thermophilus Checking the odor removal effect of the composition containing>

The effect of the composition for removing odor from livestock manure in Example 1 was confirmed in the pig farm.

Experimental example 2-1. Confirmation of odor removal effect by corrosive substances alone

In order to confirm the odor removal effect by the corrosive material alone, a composition containing 0.4% by weight of the corrosive material alone based on the total weight of the composition was sprayed inside the pighouse of a pig farm of 600 heads in Gyeonggi-do. Specifically, the pig pit is divided into three zones (zones-1 to 3) with a size of 60㎡, and a composition containing 0.4% by weight of corrosive substances based on the total weight of the composition is sprayed once a day for 5 minutes. Repeated 6 times. Ammonia, hydrogen sulfide and mercaptomethanol were measured using a gastec detector by collecting air inside the pig house before and after spraying the composition containing the corrosive substance alone, and the results are shown in FIG. 2.

As shown in FIG. 2, it was confirmed that the level of ammonia in the pig house of the pig farm was decreased after spraying the composition containing the corrosive substance alone.

Experimental Example 2-2. Confirmation of odor removal effect by Streptococcus thermophilus alone or a composition containing corrosive substances and Streptococcus thermophilus

The effect of removing odor in pig farms was confirmed by the treatment of the composition containing the corrosive substance and Streptococcus thermophilus of Example 1 or the composition containing Streptococcus thermophilus alone.

A composition containing 1.7×10 ⁹ CFU/ml of Streptococcus thermophilus alone (control) or a composition containing the corrosive substance and Streptococcus thermophilus of Example 1 in three pig farms of 600 heads in Jeollabuk-do The inside of the pig house divided by the size of 60㎡ or divided into manure slurry was sprayed with 70ℓ per week for 8 weeks, respectively. At this time, air was collected at intervals of 4 weeks and ammonia, hydrogen sulfide, and mercaptomethanol were measured using a Gastec detector. The ammonia measurement results are shown in Table 2 below, and the amount of ammonia at week 0 is 100%, and the amount of ammonia at weeks 4 and 8 is converted into percentage (NH ₃ content), and the results are shown in FIG. 3. Hydrogen sulfide and mercaptomethanol, which are other odor components, were not detected at all.

NH ₃ (ppm/100ml) Streptococcus Thermophilus Example 1 composition 0 weeks 4 weeks 8 weeks 0 weeks 4 weeks 8 weeks Pig
inside Farm A 20 10 9 20 7 5 Farm B 20 9 4 20 5 3 Farm C 15 7 9 15 3 3 excretions
Slurry Farm A 45 15 9 45 10 7 Farm B 40 20 15 40 5 5 Farm C 75 24 20 75 10 7

As shown in Table 2, compared to the case where the composition containing Streptococcus thermophilus alone was treated in both the inside of the pig house and the manure slurry, the composition containing the corrosive substance and Streptococcus thermophilus of Example 1 was treated. In one case, it was confirmed that the level of ammonia was significantly lowered.

Further, as shown in Fig. 3 in which the amount of ammonia in 0 weeks is 100% and the amount of ammonia in 4 weeks and 8 weeks in terms of percentage based on the value of ammonia in Table 2, inside the pig house (A) and manure slurry (B) Compared to the case where the composition containing Streptococcus thermophilus alone was treated, the ammonia content was lowered by 50% or more when the composition containing the corrosive substance and Streptococcus thermophilus of Example 1 was treated. Confirmed.

Through this, it is expected that the composition for removing odor of livestock manure containing the corrosive substances of the present invention and Streptococcus thermophilus can be used to remove not only the odor of livestock manure but also the odor inside the livestock shed, thereby helping to improve the livestock environment. do.

Claims (6)

A composition for removing odor from livestock manure containing a humic substance and lactic acid bacteria,
The corrosive material is a corrosive material derived from peat moss, and the lactic acid bacteria are Lactobacillus plantarum, Streptococcus thermophilus, or a mixture thereof. The method of claim 1,
The corrosive material is a composition for removing odor from livestock manure, characterized in that it contains 0.08 to 0.4% by weight based on the total weight of the composition. delete delete The method of claim 1,
The lactic acid bacteria is Streptococcus thermophilus ( Streptococcus thermophilus ) composition for removing odor from livestock manure, characterized in that. The method of claim 1,
The composition is a composition for removing odor from livestock manure, characterized in that sprayed on the inside of the livestock house or the manure slurry.

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