KR102529669B1 - On-site customized odor-removing compositions and the method of preparation thereof - Google Patents

Abstract

The present invention relates to a composition for removing malodor comprising microorganisms capable of removing malodor and adapted microorganisms collected from sites where odors occur, a method for preparing the composition, and a method for removing malodor using the same. Depending on the place of occurrence, it can have odor removal performance optimized for the site, and it can be manufactured in a relatively simple and economical way.

Description

Field-customized odor removal composition and its manufacturing method {ON-SITE CUSTOMIZED ODOR-REMOVING COMPOSITIONS AND THE METHOD OF PREPARATION THEREOF}

The present invention relates to a malodor removal composition tailored to a site capable of effectively removing malodor according to the cause and place of occurrence of the malodor, and a method for preparing the composition.

The present invention was made with the support of "Development of customized odor reduction solution to solve civil complaints of livestock farmers vulnerable to odor in Gyeonggi-do" (Task No.: D202064) of the Gyeonggi-do Technology Development Project of the Gyeonggi Business & Science Promotion Agency.

Problems such as civil complaints arise in the vicinity of facilities that generate a lot of odor, such as sewage treatment plants, composting facilities, and livestock facilities. Odor causes not only sensory discomfort, but also various symptoms such as sore eyes, nose, and throat, vomiting, dizziness, and shortness of breath. In particular, residents near odor-generating facilities or workers in related industries, who are inevitably exposed to odors for a long time, feel serious health problems due to odors. Therefore, this needs to be addressed urgently.

Substances that cause odor may include various components such as ammonia, hydrogen sulfide, methyl mercaptan, amines, volatile fatty acids, styrene, trimethylamine, methyl sulfide, methyl disulfide, acetaldehyde, propionic acid, and valeric acid. It can cause odor, but usually two or more components are mixed to cause odor. Various methods of reducing the odor may be applied depending on the substance that causes the odor. In the past, various methods such as chemical decomposition, adsorption, neutralization, masking, and sterilization have been used, but they do not remove the cause of odor but only dilute the concentration and cannot be seen as a fundamental solution. In addition, in the process of spraying or spraying a specific chemical, it may have a harmful effect on the human body and livestock, and there is a problem in that a lot of cost is required.

In a manure treatment facility installed in a livestock farm, etc., manure discharged from livestock is treated. A large amount of urea contained in urine among livestock manure is converted to ammonia by the enzyme urease. That is, a large amount of ammonia is inevitably generated in the excreta treatment facility, and this ammonia is the source of the odor. In order to reduce the ammonia, efforts are being made to increase the protein absorption rate of livestock by feeding feed additives, but the effect is insignificant. In addition, since most odor sources generate odors not only from one cause but also from a mixture of various odor-generating substances (hydrogen sulfide, methyl mercaptan, etc.), a technology for comprehensively reducing them is required.

Odor reduction technology is largely classified into physical, chemical, and biological methods depending on the treatment method based on the technical characteristics. The physical method has the advantage of not causing side effects or secondary environmental pollution, but it requires a lot of initial installation cost and has problems with repair in case of failure, so it is somewhat difficult to spread to farms. Therefore, chemical methods or biological methods are mainly used.

Regarding the chemical method, related technology is disclosed in Korean Patent Registration Publication No. 10-1671577. The above document relates to a composition for removing livestock odors containing calcium citrate, water, sodium alginate, chlorella extract, folding screen extract, magnesium hydroxide (Mg(OH) ₂ ), and magnesium oxide (MgO). Gases such as sulfur dioxide, hydrogen sulfide, methyl mercaptan (CH ₃ SH), dimethyl sulfide [(CH ₃ ) ₂ S] and amines can be removed by the composition described above.

As for the biological treatment method, it is common to use microorganisms. As a method for removing livestock odor using the above microorganisms, reference may be made to Korean Registered Patent Publication No. 10-1260872. The above document is a microbial probiotic consisting of Bacillus bacteria, Lactobacillus bacteria, Enterococcus bacteria, Streptococcus bacteria and yeast (Saccharomyces) bacteria; And a livestock odor remover containing lactic acid bacteria culture filtrate as an active ingredient. methylamine, ethylamine using the formulations described above. Odor can be eliminated by removing odor-causing substances such as trimethylamine, dimethylamine, triethylamine, hydrogen sulfide, and methyl mercaptan.

However, the above technique has a disadvantage in that it does not correspond to an effective countermeasure method according to the cause and place of occurrence of various odors. In addition, the form of the formulation does not meet the needs of the field requiring formulation in various formulations such as liquid and powder. In particular, from March 25, 2021, compulsory inspections for the ripeness of compost in livestock farms have been implemented.

Therefore, the present inventors have repeatedly studied microbial preparations that can improve the disadvantages of existing odor removal methods and reduce them in an environmentally friendly manner. After accurately identifying the cause of odor generation in various sites and studying the physicochemical properties of major odor generating substances, we can reduce them in an effective and eco-friendly way, restore contaminated soil, and promote composting. A field-customized composition for removing odor and a manufacturing method thereof have been completed.

Republic of Korea Patent Registration No. 10-1671577 (2016.10.26.) Republic of Korea Patent Registration No. 10-1260872 (2013.04.29.)

An object of the present invention is to provide a site-customized odor removal composition comprising a microorganism having an odor reducing effect by decomposing a substance causing malodor and an adapted microorganism isolated from a sample collected from the odor generating source.

Another object of the present invention is to provide a site-customized microbial preparation for removing malodor comprising the site-customized malodor removal composition.

Another object of the present invention is to provide a method for preparing the site-specific malodor removal composition and a malodor reduction method for livestock farms using the same.

The present invention is a microorganism having an odor reducing ability by decomposing a substance causing odor, a culture of the microorganism, a concentrate or dried product of the culture; and an adapted microorganism isolated from a sample collected from an odor generating source, a culture of the adapted microorganism, or a concentrate or dried product of the culture, wherein the microorganism having the odor reducing effect is Saccharomyces cerevisiae, Bacillus amyloliquis Provided is a composition for removing malodor tailored to the site, including one or more microorganisms selected from the group consisting of Paciens, Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris.

In addition, the present invention provides a site-specific microbial preparation for removing malodor comprising the above-described composition.

In addition, the present invention comprises the steps of taking a sample from the odor generating source and primary culturing the microorganisms included in the sample; Separating adapted microorganisms from among the primary cultured microorganisms and secondary culturing them in a separate medium: mixing the adapted microorganisms or their culture medium and the microorganisms having an odor reducing effect or their culture medium; Primary or secondary culture is cultured by smearing a sample taken from a source of odor on a solid medium, and the adapted microorganism is a secondary culture by separating one or two or more predominantly cultured microorganisms from the primary cultured sample. Microorganisms having an odor-reducing effect are selected from the group consisting of Saccharomyces cerevisiae, Bacillus amyloliquifaciens, Bacillus subtilis, Bacillus licheniformis, and Rhodopseudomonas palustris. , Provides a method for manufacturing a site-specific microbial deodorant.

In addition, the present invention provides a method for removing odor by incubating the above-described composition for removing odor together with sampled wastewater, livestock manure, compost, or household waste.

The present invention relates to a composition for removing odor containing adapted microorganisms collected from a site where odor is generated, and the composition has an effect of removing odor optimized according to the cause and location of the odor. In particular, the composition of the present invention, as a microbial agent, can improve the harmfulness caused by chemical control to remove odors in an eco-friendly and economical way. In addition, even when a plurality of odor-causing components are mixed, it can be effectively removed, and it can be formulated into various formulations such as liquid and powder.

1 is a diagram showing a step-by-step process for preparing a composition for removing odor according to the present invention.
Figure 2 shows the primary culture and secondary separation and culture processes of odor generating source samples for isolating the adapted microorganisms of the present invention.
3 shows a process of shaking and culturing adapted microorganisms isolated from a sample of an odor generating source.
Figure 4 shows the state of manure before / after application of adapted microorganisms.
Figure 5 shows the process of isolating adapted microorganisms by smearing after serial dilution.

Hereinafter, the present invention will be described in more detail.

The present invention is a microorganism having an odor reducing ability by decomposing a substance causing odor, a culture of the microorganism, a concentrate or dried product of the culture; and an adapted microorganism isolated from a sample collected from an odor generating source, a culture of the adapted microorganism, or a concentrate or dried product of the culture, wherein the microorganism having the odor reducing effect is Saccharomyces cerevisiae, Bacillus amyloliquis It relates to a composition for removing malodor tailored to the site, comprising at least one microorganism selected from the group consisting of Paciens, Bacillus subtilis, Bacillus licheniformis, and Rhodopseudomonas palustris.

In the present specification, the term 'customized to the site' includes a meaning optimized according to various sources of odor generation, and the composition for eliminating odor tailored to the site of the present invention is an adapted microorganism obtained from a sample collected from a source of odor in the field. By including in the malodor removal composition, it is possible to maximize the malodor removal effect of the malodor generating source. In general, in livestock manure, various salts such as zinc and copper, which participated in feed for livestock growth, accumulate and show high salt concentration. In high salt concentrations, it is not easy to grow general strains other than salt-tolerant strains. Therefore, the adapted microorganisms obtained in the field are the most suitable strains in the in-situ environment (high salt concentration), and through the dominance of these strains, decomposition of organic matter such as livestock manure promotes ripening and reduces odor.

In this specification, the term 'odor source' refers to various wastes, manure, etc., including odor-causing substances, and the 'odor-causing material' is a substance that stimulates the sense of smell and causes discomfort and aversion, mainly irritating gases. means The odor-causing substances mainly belong to volatile compounds, and may include nitrogen compounds, sulfur compounds, lower fatty acids, carbonyl compounds, esters, phenols/cresols, alcohols, hydrocarbons, chlorine compounds, and the like. Although not limited thereto, the odor-causing substance of the present invention may be a nitrogen compound or a sulfur compound, and more preferably may include ammonia, hydrogen sulfide, methyl sulfide, methyl mercaptan, or amines.

One component of the present invention, the 'microorganism having an odor reducing ability', has a function of removing odor by decomposing one or more of the odor-causing substances. The microorganism having the odor reducing ability is not limited to the type, but in the present invention, Saccharomyces cerevisiae , Bacillus amyloliquefaciens , Bacillus subtilis ( Bacillus subtilis ) , Bacillus licheniformis ( Bacillus licheniformis ) and Rhodopseudomonas palustris ( Rhodopseudomonas palustris ).

As used herein, the term 'adapted microorganism' refers to a microorganism that is dominant in a culture obtained by culturing a sample taken from an odor generating source, and refers to a species with a large number of colonies formed by microorganisms in the culture and a large area. . The adapted microorganisms may be one or two or more. The use of adapted microorganisms is to improve the decomposition of organic matter and the removal of odor by isolating and culturing microorganisms that decompose well the organic matter at the site where the odor occurs. Odor generating sources include various microorganisms that reproduce using organic substances as nutrients depending on the type of organic matter contained in the waste, so when they are included in the composition together with odor removing microorganisms, the decomposition of odor generating substances at the site is more effective. Accordingly, the odor reduction effect can be greatly improved.

The composition of the present invention may include the microorganisms as microorganisms themselves, or may be included in a culture medium of the microorganisms, a dried product or a concentrated solution of the culture medium, and may be included in a liquid or solid form. The culture medium or dried material of the culture medium means that a specific microorganism is cultured in a culture medium or culture medium, and it is preferable that the microorganism is contained in the culture medium.

The composition of the present invention is not limited thereto, but the ratio of the microorganism having the malodor reducing ability or the culture solution of the microorganism to the adapted microorganism or the culture solution thereof may be included in the composition at a ratio of 1:1 to 6:1. The ratio may be adjusted differently according to the type of the causative substance at the site of odor generation.

In addition, with respect to the total composition, the microorganism or microbial culture may be included in 10 to 90% by weight, more preferably 10 to 60% by weight. When the content of microorganisms satisfies the above range, it is economically desirable and has an excellent odor removal effect. Specifically, the composition according to the present invention may be used by properly changing the microorganism or microbial culture medium within the above range according to the type of odor in the field.

In addition, the composition of the present invention may further include, in addition to the microorganisms, a material that serves as a nutrient source for microorganisms in order to stabilize the microorganisms, and the nutrient source is not limited to those components, but glucose, molasses, humic acid, rice bran and It may contain one or more of the amino acids. In one embodiment of the present invention, glucose, molasses or amino acids were mixed and used.

In the present invention, the composition is not limited to its dosage form, but may be formulated into a powder, pellet granule, or solution by mixing with a commonly used carrier. The carrier may be water, celite, zeolite, fillite, vermiculite, peat moss, talc, kaolinite, bentonize, pig dust, fish dust, chicken dust, rice dust, rappe dust, bone dust, and kaolin. It is not limited thereto.

1 is a diagram illustrating a step-by-step manufacturing process of a composition for removing malodor of the present invention. For example, referring to FIG. 1, a medium necessary for culturing microorganisms is prepared, sterilized and sterilized, cultured microorganisms in a mass incubator under culture conditions for each microorganism, and then the density and degree of contamination of the cultured microorganisms can be examined. . Thereafter, a composition for removing odor can be prepared by adding powdered raw materials to the product container according to the mixing ratio, adding liquid raw materials to the product container according to the mixing ratio, and then attaching a sticker to the product that has been manufactured. The composition for removal can be stored in a storage room until the product is shipped.

The present invention also relates to a microbial preparation for removing malodor tailored to the site comprising the above-described composition.

The preparation may be a powder, dry matter, liquid or concentrate of the composition, but is not limited thereto.

Specifically, the composition for removing malodor of the present invention may be mixed with a commonly used carrier or excipient and formulated in the form of a liquid, concentrate, dry material or powder. The preparation in solid form may be commercialized in the form of beads, powders, or granules after attaching microorganisms to a carrier, drying the water content to 0.1 to 10% by weight, and the like.

The solid formulation may further include an excipient, and the excipient may be used alone or in combination of two or more of starch, amino acid, vitamin C, vitamin E, chitosan, and glucose, but is not limited thereto.

The present invention also includes the steps of taking a sample from the odor generating source and primary culturing the microorganisms included in the sample; Separating adapted microorganisms from among the primary cultured microorganisms and secondary culturing them in a separate medium: mixing the adapted microorganisms or their culture medium and the microorganisms having an odor reducing effect or their culture medium; Primary or secondary culture is cultured by smearing a sample taken from a source of odor on a solid medium, and the adapted microorganism is a secondary culture by separating one or two or more predominantly cultured microorganisms from the primary cultured sample. Microorganisms having an odor-reducing effect are selected from the group consisting of Saccharomyces cerevisiae, Bacillus amyloliquifaciens, Bacillus subtilis, Bacillus licheniformis, and Rhodopseudomonas palustris. , It relates to a method for manufacturing a site-specific microbial deodorant.

As described above, the adapted microorganisms refer to microorganisms that are predominant in samples collected from odor sources in the field to be deodorized. In the case of a general pig farm, livestock are raised on the ground of the barn and pig manure is stored in a slurry pit under the barn. Livestock feed contains various salts such as phosphates, calcium salts, and inorganic materials for growth, and many salts accumulate in the slurry pit after eating these feeds. Therefore, there arises a problem that general microorganisms do not grow in a slurry pit having a high salt concentration. However, the site-specific microbial deodorant according to the present invention can provide microorganisms having salt tolerance by isolating and culturing adapted microorganisms in the field.

According to one embodiment of the present invention, in order to isolate the adapted microorganisms, the sample is first smeared and cultured on a solid agar medium, and then dominant bacteria having a large number of colonies visually are secondarily separated and cultured on a solid agar medium. did The isolated and cultured adapted microorganisms may be one or two or more species, and the isolated and cultured adapted microorganisms were transferred to a liquid medium and then shaken and cultured for use (see FIG. 3 ).

The dominant adapted microorganism is confirmed by making a sample by serial dilution and spreading it on a bacterial medium TSA. Color, colony shape, smell, etc. are visually checked and the most bacteria in the agar medium are called adapted microorganisms. These adapted microorganisms are passaged and stored as single colonies. 5 shows the process of separating adapted microorganisms by smearing after serial dilution.

The present invention also relates to a method for removing odor by incubating the above-described composition for removing odor together with wastewater, livestock manure, compost, or household waste from a sampled odor source.

In the case of using the composition for removing odor according to the present invention, odor can be reduced without secondary contamination, and thus there is an effect of soil improvement and environmental improvement by using composted liquid manure and compost.

Hereinafter, examples will be described in detail in order to specifically describe the present specification. However, the embodiments according to the present specification may be modified in many different forms, and the scope of the present specification is not construed as being limited to the embodiments described below. The embodiments herein are provided to more completely explain the present specification to those skilled in the art.

Experimental example 1. Stink reducing ability Cultivation of eggplant microorganisms

1-1. bacillus licheniformis SF5-1( Bacillus licheniformis ) culture

Bacillus licheniformis SF5-1 strain (KACC 91618P) was distributed and mass-cultivated through an incubator under aerobic conditions in a medium having the composition shown in Table 1 below. The incubator used a 2,000L capacity incubator, and was cultured for 24 hours under conditions of an optimal temperature of 35℃ and an air injection rate of 150L/min. After culturing, it was concentrated and lyophilized, if necessary, to prepare a powder formulation and store it. This strain is known to have proteolytic ability (see Patent No. 10-1278617).

badge raw material content, % Glucose 0.5 Peptone 1.0 Yeast extract 0.5 KH2PO4 0.3

1-2. bacillus Amyloliquifaciens AEY -One( Bacillus amyloliquefaciens ) culture

Bacillus amyloliquefaciens AEY-1 strain (KCTC 18314P) was received and cultured under aerobic conditions in a medium having the composition shown in Table 2 below. The incubator used a 2,000L capacity incubator, and was cultured for 24 hours under conditions of an optimal temperature of 35℃ and an air injection rate of 150L/min. After culturing, it was concentrated and lyophilized, if necessary, to prepare a powder formulation and store it. The strain is a strain known to have an ammonia removal effect (see Patent No. 10-1799750).

badge raw material content, % Glucose 0.5 Peptone 1.0 Yeast extract 0.5 KH ₂ PO ₄ 0.3

1-3. Rhodopseudomonas palustris JK -One( Rhodopseudomonas palustris ) culture

Rhodopseudomonas palustris JK-1 strain (KACC 92045P) was received, and a medium having the composition shown in Table 3 below was used. A microbial incubator with a capacity of 2,000 L was used, and 20 kg (2%, w / v) of the medium amount was put into a working volume of 1,000 L, and sterilization was used, and anaerobic culture was performed under optimal conditions of 30 ° C and light conditions. The strain is photosynthetic and is effective in decomposing hydrogen sulfide.

badge raw material Based on 1L, g Yeast extract 0.2 Sodium lactate 1.0 MgSO ₄ 0.2 NaCl 0.2 CaCl ₂ 0.05 KH ₂ PO ₄ 0.5 NH _4Cl 0.5

1-4. Saccharomyces Celebrity UB28-30( Saccharomyces cerevisiae ) culture

Saccharomyces cerevisiae UB28-30 is a strain previously owned by the applicant, and was mass-cultured through an incubator at 35 ° C. in aerobic conditions in a medium having the composition shown in Table 4 below. did The incubator used a 2,000L capacity incubator, and was cultured for 36 hours under the conditions of an optimal temperature of 35℃ and an air injection amount of 100L/min. After culturing, it was concentrated and lyophilized, if necessary, to prepare a powder formulation and store it. The strain has a degrading ability for organic substances such as proteins.

badge raw material content, % molasses 10 Corn Steep Liquor (CSL) 4.0 Yeast extract 0.5 Glucose 0.5 KH ₂ PO ₄ 0.3

1-5. bacillus subtilis UB -156( Bacillus subtilis ) culture

Bacillus subtilis UB-156 is a strain already possessed by the applicant, and was mass-cultured through an incubator under aerobic conditions in a medium having the composition shown in Table 5 below. The incubator used a 2,000L capacity incubator, and was cultured for 24 hours under conditions of an optimal temperature of 35℃ and an air injection rate of 150L/min. After culturing, it was concentrated and lyophilized, if necessary, to prepare a powder formulation and store it. The strain has an ability to decompose organic matter such as protein and ammonia.

badge raw material content, % Glucose 0.5 Peptone 1.0 Yeast extract 0.5 KH ₂ PO ₄ 0.3

Experimental example 2. Isolation and cultivation of adapted microorganisms

2-1. Field Customizing Agar, FCA )

Adaptive microorganisms are isolated by taking samples from odor sources at the site of odor generation and culturing them. First, to 1.2 g of solid agar powder, samples (liquid fertilizer) collected from each odor generating site were diluted in distilled water at a rate of 5% and added, sterilized at 121 ° C for 15 minutes, and then dispensed. cultured (primary culture).

division Input amount based on 100ml Distilled water 95ml On-site liquid manure and compost 5ml Agar powder 1.2g

2-2. Separate culture of adapted microorganisms (secondary culture)

A sample was made at an appropriate concentration through serial dilution in the field-customized medium (FCA) prepared in 2-1 above, and it was smeared on a FAC medium and cultured in an incubator at 30 ° C. for 2 to 3 days. Among various bacteria, bacteria with similar colony shape, color, smell, etc. were identified, and the bacteria showing the largest population were isolated and re-cultured. As the culture medium, TSA (Tripic Soy Agar) or NA (Nutrient Agar) medium was used as a solid agar medium. As shown in Figure 2, the primary and secondary culture results of the adapted microorganisms were obtained.

2-3. Mass culture of adapted microorganisms

Each of the adapted microorganisms obtained as a result of the separate culture in 2-2 was inoculated into a liquid medium and cultured with shaking (see FIG. 3). The isolated and cultured adapted microorganisms were inoculated in a TSB (Tripic Soy Broth) medium, shaken and cultured for 2 to 3 days at 35° C. and 150 rpm and stored.

Experimental example 3. Preparation of site-specific deodorant composition

With the following composition, Examples and Comparative Examples were prepared. Microorganisms and adapted microorganisms having odor reduction ability cultured in Experimental Examples 1 and 2 were mixed, and nutrient sources, carriers, and additives were prepared according to the ratios shown in Table 7 below. (Examples 1 to 3, Comparative Examples 1 to 3)

The test sites were 1. Nonghyup composting facility in Goesan, Chungcheongbuk-do, 2. Pig farm in Cheorwon, Gangwon-do, and 3. Pig farm in Gochang, Jeonbuk. used after.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 brewer's yeast 50 50 50 - - - Saccharomyces Serviziae UB28-30 10 10 10 20 20 20 Bacillus subtilis UB156 10 10 10 20 20 20 Bacillus licheniformis SF5-1 10 - 10 - - - Bacillus amyloliquifaciens AEY-1 10 - - 20 - - Rhodopseudomonas palustris JK-1 - 15 - - 20 10 molasses 10 10 10 - - - glucose - - - 10 10 10 zeolite - - 5 - - 5 amino acid - 5 5 - 5 5 Lactic acid bacteria ( L. Plantalum ) - - - 20 15 20 adapted microbes - - - 10 10 10 Sum 100 100 100 100 100 100

Experimental example 4. Stink reduction Check the effect

After spraying the composition of Example and Comparative Example prepared from Experimental Example 3 on the site, the change in concentration of the odor-causing substance according to the type of odor-generating source was measured. The odor was measured by measuring the type and concentration through a Gastech detector and an odor meter.

As a result, as shown in Table 8 below, it was confirmed that the concentration of odor-causing substances was greatly reduced in the case of the composition containing the adapted microorganisms of the present invention for each site, and based on these results, when the adapted microorganisms were included, It can be confirmed that the odor reduction effect according to the site is more excellent.

smell classification site name recipe Before treatment (ppm) After treatment (ppm) ammonia dominance Chungbuk Goesan
00Nonghyup Composting Ground Recipe number ① NH ₃ 470 NH ₃ 50 hydrogen sulfide dominance Gangwon Cheorwon
00Farm Recipe number ⑤ _{H2S253 H2S60} complex odor Jeonbuk Gochang
00Farm `Recipe number ⑥ NH ₃ 100
H ₂ S 100
_CH4S10 NH ₃ 3
H ₂ S 0.2
_CH4S0

In addition, the odor removal composition of the present invention containing adapted microorganisms is formulated as follows (product name: Saccharo) and sprayed on various sites, and then, after 3 days, the concentration of odor-causing substances is measured through a gas detection tube (Gastech) and was measured through an odor meter. Figure 4 is a diagram showing the state of liquid manure (manure) before and after spraying adapted microorganisms. As a result, it was confirmed that the concentrations of various odor-causing substances were significantly reduced as shown in Table 9. In addition, even with the naked eye, it can be seen that black manure is well fermented in the early stage of liquid manure and turns brown or dark brown. There are various ways to judge the ripeness of compost and liquid manure, such as smell, color, indication by analysis using a gas meter, pH, and electrical conductivity (EC). In the field, the method of judging based on color is widely used because it is the easiest and fastest. The liquid manure is black gray or dark black at the beginning, dark brown in the middle of ripening, and dark brown or brown in the late and complete ripening.

site scene 1 scene 2 scene 3 scene 4 scene 5 scene 6 scene 7 scene 8 scene 9 causative agent hydrogen sulfide methyl mercaptan ammonia ammonia ammonia ammonia ammonia ammonia ammonia ammonia Before spraying 16.0 9.9 25 60 18 25 84 62 75 58 After spraying 0.0 0.4 9 17 14 9 40 20 25 3

(Concentration: ppm)

So far, the present invention has been looked at with respect to its preferred embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent range should be construed as being included in the present invention.

Claims (5)

microorganisms having an odor reducing ability by decomposing odor-causing substances, cultures of the microorganisms, concentrates or dried products of the cultures; and
Including an adapted microorganism isolated from a sample collected from an odor generating source, a culture of the adapted microorganism, and a concentrate or dried product of the culture,
The microorganism having the odor reducing effect includes at least one microorganism selected from the group consisting of Saccharomyces cerevisiae, Bacillus amyloliquifaciens, Bacillus subtilis, Bacillus licheniformis and Rhodopseudomonas palustris,
Wherein the adapted microorganism is obtained by culturing a sample taken from the odor source and isolating and culturing one or more microorganisms dominant in the culture medium.
delete A field-customized microbial agent for removing malodor for use in a malodor generating source from which a sample containing the composition of claim 1 is taken. Collecting a sample from an odor generating source and primary culturing microorganisms included in the sample;
Separating adapted microorganisms from among the primary cultured microorganisms and secondary culture in a separate medium: and
Mixing the adapted microorganism or its culture medium and the microorganism having an odor reducing effect or its culture medium,
The primary or secondary culture is cultured by smearing a sample collected from an odor generating source on a solid medium,
The adapted microorganisms are secondary cultures obtained by isolating one or two or more predominantly cultured microorganisms from the primary cultured samples,
The microorganism having the odor reducing effect is selected from the group consisting of Saccharomyces cerevisiae, Bacillus amyloliquifaciens, Bacillus subtilis, Bacillus licheniformis, and Rhodopseudomonas palustris. A method for manufacturing a site-specific microbial deodorant for use in a sampled odor source. A method of removing odor by incubating the composition for removing odor of claim 1 together with wastewater, livestock manure, compost, or household waste from a source of odor.

Images