KR101671577B1 - Production method of Antibacterial livestock deodorant composition - Google Patents

Abstract

The present invention relates to a method for producing a composition for removing animal offensive odor, wherein the production method of the present invention efficiently removes gas such as hydrogen sulfide, ammonia, or amines generated in livestock or poultry, It is possible to effectively produce a composition which exhibits harmless antibacterial or fungicidal activity on livestock to improve the livestock environment.

Description

TECHNICAL FIELD The present invention relates to a method for producing an antimicrobial composition for removing animal odor,

The present invention relates to a method for producing a composition having antimicrobial and bactericidal activity for the removal of animal offensive odors, which is harmless to livestock and cattle using natural materials. The method of the present invention is a method for producing a composition containing hydrogen sulfide, ammonia or amines It is possible to effectively improve the livestock environment in a nature-friendly manner by providing a method of efficiently removing the gas of natural materials and having antibacterial or bactericidal activity of natural materials.

Meat consumption has been increasing with the improvement of people's living standards and dietary life. This change has been accompanied by the positive effects of the expansion of the livestock industry and the improvement of the technology level, as well as the overcrowding of unit farms due to the quantitative growth of commercial livestock production, .

The increase in livestock manure has been extended to the secondary problems such as odor, water quality and soil pollution caused by manure as well as throughput. In particular, Korea has a short distance between housing and housing, And odor generation are particularly problematic.

The odor from the livestock facility has become a source of bad complaints between the farmers and the surrounding residents, and efforts should be made to reduce the excretion of these pollutants and nitrogen. Above all, it is necessary to effectively control and alleviate harmful fungi that cause ill-smelling gases such as volatile fatty acids, ammonia and volatile amines, indole groups, phenol and sulfur compounds, and livestock that cause environmental pollution inside the house .

Since the enforcement of the Anti-Odor Control Law (March 2008), various odor reduction technologies and facilities have been developed and distributed to farmers, but the odor handling capacity is different among farmers using similar technology facilities. Development of abatement technology is not easy.

Unlike factories, all facilities are exposed to the outside, so there is a very limited way to reduce odors, and the cost of odor prevention facilities is considerably high, making it difficult to install preventive facilities on their own The supply to the farmhouse is also weak.

The technology of odor reduction is divided into the viewpoint based on the technical characteristic or the view point based on the generation stage or the place where the odorous substance is generated. In the case where it is based on the technical characteristic, it is divided into physical, chemical, Chemical methods include oxidation, ozone chlorination, chemical cleaning, ion exchange, combustion, neutralization, and masking. Physical methods include water washing, activated carbon adsorption, cooling, air dilution and ball blocking. Examples of methods include a liquid contact method and a meat contact method. In addition, it can be divided into absorption method, adsorption method, illustration method, and biological deodorization method according to the control technique.

However, in the case of the above-mentioned masking method, the effector is limited in a short time because it does not completely remove the odor-causing substance but merely blocks it, and it can cause irritation to the human body due to toxicity of the used material. There is a problem that the collected odorous substances may be released again with the lapse of time. In addition, in the case of the chemical deodorization method, the odor removing effect has not yet reached a satisfactory level, and the chemical substances used may be harmful to the human body and the environment. In addition, in the case of the biological deodorization method using microorganisms most commonly used in poultry houses and the like, there is a large difference in activity depending on the surrounding environment, so it is difficult to expect a stable efficacy and the odor and odor- And is released into the atmosphere.

Currently, the above-mentioned conventional techniques are used formally in livestock farmhouses other than regularly cleaning the interior of the barn. However, since the decomposition gases such as ammonia and hydrogen sulfide generated in the livestock odor and shale fragments, which continuously occur in the barn, It is impossible to control it. In some livestock farms, microbial fermentation methods are used in parallel, but there is a limit to the biological methods as described above, and an alternative method is urgently needed.

Accordingly, the present inventors have found that the use of an environmentally friendly substance that is safe, non-irritating, and non-toxic to livestock kept in a house can effectively remove odor causing substances and exhibit excellent antibacterial and bactericidal activity Antibacterial, sterilizing, and odor removing agents.

It is an object of the present invention to provide a method for producing a composition which can effectively remove odors generated in livestock and housing using natural substances harmless to the surrounding environment or living organism.

In order to achieve the above object, the present invention provides a process for preparing a calcium carbonate, comprising: i) mixing calcium citrate with water, activating it for 8 to 36 hours, and then removing the precipitate to form a solution; Ii) mixing the solution formed in step i) with sodium alginate, chlorella extract, and screen wind extract, aging for 8 to 36 hours, and then removing the precipitate to form a solution; Of the present invention.

In one embodiment of the present invention, magnesium hydroxide (Mg (OH) ₂ ) or magnesium oxide (MgO) is mixed with water between the step i) and the step ii) Removing the precipitate to form a solution, and mixing the thus formed solution with the solution formed in step i) to form a mixed solution.

In one embodiment of the present invention, the composition comprises 0.01 to 25 parts by weight of sodium alginate relative to 100 parts by weight of the total composition; 0.01 to 5 parts by weight of chlorella extract; 0.01 to 5 parts by weight of a screen wind extract; 0.1 to 10 parts by weight of calcium citrate; And water of the remainder.

In one embodiment of the present invention, the mixing of step ii) may further comprise mixing of fragrance and / or minerals.

In one embodiment of the present invention, the composition is one or more selected from the group consisting of ammonia, sulfur dioxide, hydrogen sulfide, methyl mercaptan (CH ₃ SH), dimethyl sulfide [(CH ₃ ) ₂ S] The gas can be removed.

In one embodiment of the present invention, the composition may exhibit an antibacterial or bactericidal activity. For example, the composition may exhibit antibacterial or bactericidal activity against at least one strain selected from the group consisting of E. coli, Salmonella, Pseudomonas, Clostridium, Staphylococcus, Enterococcus, but is not limited thereto.

The method of the present invention for producing an animal offensive odor removing composition effectively removes a malodor originating from a house or livestock, as well as effectively producing an animal offensive odor removing composition exhibiting antibacterial or bactericidal activity against pathogenic bacteria.

A method for preparing an animal offensive odor removing composition according to one aspect of the present invention comprises the steps of: i) mixing calcium citrate with water, activating it for 8 to 36 hours, and then removing the precipitate to form a solution; Ii) mixing sodium alginate, chlorella extract, and screen wind extract with the solution formed in step i), aging the mixture for 8 to 36 hours, and removing the precipitate to form a solution.

In the present invention, the sodium alginate, the chlorella extract, and the screen wind extract are natural extracts obtained from nature, and they absorb and remove odor gas. The calcium citrate interacts with the sodium alginate, the chlorella extract, and the screen wind extract. The calcium citrate reacts with ammonia, trimethylamine, hydrogen sulfide, sulfur dioxide, methyl mercaptan, dimethyl sulfide and the like, And discharging it. The sodium alginate, the chlorella extract, the screen wind extract and the calcium citrate are excellent in safety against the human body and the environment and can exert excellent deodorizing, antibacterial and sterilizing effects through interaction with each other.

In one example of the present invention, the method for preparing a composition for removing animal odor comprises mixing magnesium hydroxide (Mg (OH) ₂ ) or magnesium oxide (MgO) with water between step (i) , Activating for 8 to 36 hours, removing the precipitate to form a solution, and mixing the thus formed solution with the solution formed in step i) to form a mixed solution.

When the magnesium hydroxide (Mg (OH) ₂ ) or magnesium oxide (MgO) is further included, it may be contained in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of the entire composition.

When the composition further contains magnesium hydroxide (Mg (OH) ₂ ) or magnesium oxide (MgO) as described above, ammonia, trimethylamine, hydrogen sulfide, sulfur dioxide, methyl mercaptan, dimethyl sulfide, Thereby accelerating the action of generating a water-soluble substance.

Scheme _{1: 2NH 3 + Mg (OH} ) 2 + O 2 → Mg (NO 3) 2 + 4H 2 O

Scheme _{2: (CH 3) 3 N} + Mg (OH) 2 → (CH 3) 2 NOMgO

Reaction formula 3: H ₂ S + Mg (OH) ₂ + O ₂ ? MgS + 2H ₂ O

Reaction 4: SO ₂ + Mg (OH) ₂ + _{1 /} 2O ₂ → MgSO ₄ + H ₂ O

Scheme _{5: 2CH 3 SH + 2Mg (} OH) 2 → 2CH 3 SMg + 3H 2 O + 1/2 O 2

Scheme _{6: (CH 3) 2 S} + 2Mg (OH) 2 (CH 3) 2 SO 3 + 2MgO + 2H 2 O

In one embodiment of the present invention, the composition comprises 0.01 to 25 parts by weight of sodium alginate relative to 100 parts by weight of the total composition; 0.01 to 5 parts by weight of chlorella extract; 0.01 to 5 parts by weight of a screen wind extract; 0.1 to 10 parts by weight of calcium citrate; And the remainder of water. When the content of the sodium alginate, chlorella extract, and screen wind extract is less than 0.01 part by weight, the peeling and coagulation effect of a substance causing odor or a contaminant is not sufficiently exhibited, so that the antibacterial, sterilizing and deodorizing activity is difficult to be sufficiently exhibited, , There may occur a problem of miscibility with other constituents, and it is difficult to expect further improvement of the effect according to the increase of the content.

In one embodiment of the present invention, the mixing of step ii) may further comprise further mixing of natural perfume ingredients. The fragrance may be selected from the group consisting of lemon oil, rose oil, lavender oil, Vegamot oil, Western peppermint oil, eucalyptus oil, geranium oil, clove oil, cinnamon oil, orange oil, jasmine oil, rosemary oil, aniseed fruit oil, peppermint oil, , Ylang-ylang oil, 1,8-cinereol, menthol, terpinol hydrate, limonene, a-pinene, and ergeneol.

Also, in one embodiment of the present invention, the mixing of step ii) may further include mixing of mineral components. Korean Patent No. 10-1087052, for example, discloses a method for producing an animal offensive odor remover prepared by adding a liquid complex mineral extracted from granite, wherein the complex mineral is selected from the group consisting of lithium, sodium, magnesium, aluminum, , Calcium, titanium, vanadium, manganese, iron, cobalt, nickel, copper, zinc germanium, and rubidium.

The method for producing a composition for removing animal offensive odor of the present invention can effectively produce a composition having an effect of decomposing and removing odor causing substances and causative bacteria. Examples of the odor-causing substances include, but are not limited to, ammonia gas and amines such as methyl mercaptan (CH ₃ SH), dimethyl sulfide [(CH ₃ ) ₂ S], hydrogen sulfide (H ₂ S), trimethylamine (CH ₃ ) ₃ N, methyl sulfide (CH ₃ ) ₂ S, acetaldehyde (CH ₃ CHO), methyl disulfide ((CH ₃ ) ₂ S ₂ ), styrene (C ₈ H ₈ ) Contains all the odorous substances present.

The causative microorganisms include not only bacteria but also fungi and viruses, as well as microbes causing illness in livestock as well as causes of odor. Examples of the bacteria include, but are not limited to, Salmonella, Clostridium, Escherichia coli, Staphylococcus, Enterococcus, Pseudomonas, and Mycobacterium. Examples of fungi include tricophyton, Candida and Aspergillus Examples of viruses include avian influenza, foot and mouth disease, swine cholera, African swine fever, rabies, and Newcastle virus.

The composition for removing malodor can be used in the form of a powder or an aqueous solution according to an application method, and in the case of powder, an appropriate particle size can be selected in consideration of reactivity. When used as an aqueous solution, the pH range of the aqueous solution may be 6.0 to 11.0, preferably 7.0 to 9.0, but is not limited thereto. In general, a conventionally known antimicrobial agent exhibits a strong alkalinity of pH 12 or more, and thus is known to exhibit antibacterial properties. However, the composition of the present invention can exhibit not only superior antibacterial and sterilizing effects but also excellent odor removing effect while exhibiting much lower pH. Further, the pH value is maintained in the range of almost neutral or slightly alkaline, thereby further securing safety and ease of handling.

Hereinafter, the present invention will be described in more detail by way of examples. However, the following examples are illustrative of the present invention, and the scope of the present invention is not limited to the following examples.

Example  1. Preparation of composition for removing animal odor according to the present invention

Sodium alginate (a), chlorella extract (b) and screen wind extract (c) were mixed according to the preparation example shown in the following Table 1, and a mixed solution was prepared.

Also, calcium citrate (d); And / or magnesium hydroxide (e) were each mixed with water, stirred well, allowed to stand for about 24 hours, and the precipitate was removed to form a solution, respectively. The thus prepared calcium citrate solution, a mixed solution of calcium citrate and magnesium hydroxide were mixed and stirred, and a mixed solution of sodium alginate (a), chlorella extract (b) and screen wind extract (c) Respectively. The agitated solution was allowed to stand for about 24 hours, and the precipitate was removed to prepare a composition according to the present invention. On the other hand, comparative compositions were also prepared in a similar manner and compared to compositions according to the present invention. The composition and content of the prepared composition are shown in Table 1.

[Table 1] Production example

Example  2. Deodorizing effect by animal odor component

<2-1> Sensory evaluation

Deodorizing effect was observed using trimethylamine, ammonia, hydrogen sulfide and sulfur dioxide, which are the causative substances of odor generated in the housing, using the compositions of Preparation Examples 1 to 6 and Comparative Examples 1 to 4 prepared in 1 above (Table 2) . After spraying the composition on each of the causative substances, odor before and after spraying was observed by smell and classified according to the following criteria.

?: Odor removed or significantly decreased after spraying.

DELTA: Odor slightly decreased after spraying.

X: Odor after spraying hardly decreased.

[Table 2] Sensory evaluation

As a result of the above-mentioned experiment, it was confirmed that the composition according to the present invention exhibits a reliable deodorizing effect for each of the causative substances. However, in the case of Comparative Examples 1 to 4, the deodorizing effect was not exhibited or the effect was lower than that of the products of Production Examples 1 to 6, depending on the odor.

<2-2> Measurement of deodorizing effect over time

In order to objectively confirm the sensory evaluation result of the above-mentioned <2-1>, the inventors of the present invention found that the gas detection tube method (KS I 2218) described in the registered patent No. 10-1260872, (Table 3 and Table 4). 20 ml of a liquid sample of each of the compositions of Preparation Examples 1 and 6 was placed in a 5-liter reactor and sealed. The initial concentration of the test gas was injected with 100 μmol / mol (ppm) of ammonia and 30 μmol / mol (ppm) of trimethylamine and the concentration of the test gas was measured at the initial (0 minute), 30 minute, 60 minute, 90 minute and 120 minute This was called sample concentration. During the test, the temperature was maintained at 23 ° C ± 5 ° C and the humidity was maintained at 50% ± 10%. Separately, in the absence of a sample, the same test as described above was carried out, which was called blank concentration. The removal rate of test gas for each time period was calculated by the following equation.

Removal rate (%) = [{ blank  Concentration) - (concentration of sample) / ( blank  Concentration)} X 100

[Table 3] Ammonia (NH ₃ ) deodorization test

[Table 4] Triethylamine (CH ₃ ) ₃ N) Deodorization test

As described above, when the composition for removing odor according to the present invention was treated with ammonia and triethylamine, the odor removal rate reached almost 100% within 30 minutes, and this result supports the above sensory evaluation result.

Example  3. Odor removal experiment

1 kg of pig manure, which generates about 10 ppmv of ammonia and amine, was put into an acrylic reaction tank of about 96 L (40 cm X 40 cm X 60 cm), and the composition for removing animal odor of Example 1 was set at 10, 20, 30, 60, 100, and 200 cc, and then mixed with the impeller at 70 rpm. After 1, 2, 12, and 24 hours, the ammonia contained in the odor substances was removed by the moisture absorption method And amines were measured with a detector tube. As a result, it was confirmed that a very high deodorizing effect can be obtained when spraying 40 cc or more as a whole.

Example  4. Antimicrobial activity measurement

<4-1>

Seven kinds of bacteria (Escherichia coli, Salmonella, Pseudomonas, Clostridium, Streptococcus, Enterococcus and Mycobacterium) isolated from the bottom of the pork were cultivated in a culture dish and then treated in the same manner as in Preparation Examples 1 to 6 and Comparative Examples 1 to 3 And the degree of growth inhibition was measured (Table 5).

[Table 5]

As a result, the antibacterial activity of the composition of the present invention was confirmed as a whole although there was a difference depending on the kind of the subject bacteria.

<4-2>

Pig manure was applied to each reagent bar and the bacterial counts were measured by a bacterial analyzer (Clean-Q, Model TBD 1000, Teltel). The bacterial count of pig manure was 6456.

[Table 6]

From the results, it was confirmed that the compositions of Production Examples 1 to 6 exhibited excellent antibacterial and germicidal effects as compared with Comparative Examples.

Claims (7)

I) mixing calcium citrate with water, activating for 8 to 36 hours, and then removing the precipitate to form a solution;
Ii) mixing the solution formed in step i) with sodium alginate, chlorella extract, and screen wind extract, aging for 8 to 36 hours, and removing the precipitate to form a solution,
Said composition comprising 0.01 to 25 parts by weight of sodium alginate relative to 100 parts by weight of the total composition; 0.01 to 5 parts by weight of chlorella extract; 0.01 to 5 parts by weight of a screen wind extract; 0.1 to 10 parts by weight of calcium citrate; And water of the remainder,
Wherein the pH of the solution formed by removing the precipitate of step ii) is 6.0 to 11.0. The method according to claim 1,
Between the step i) and the step ii)
After the magnesium hydroxide (Mg (OH) ₂ ) or magnesium oxide (MgO) is mixed with water and activated for 8 to 36 hours, the precipitate is removed to form a solution, To form a mixed solution. The method for preparing a composition for removing animal odor according to claim 1, delete The method according to claim 1,
Wherein the mixing of step (ii) further comprises adding a perfume or a mineral component. 5. The method according to any one of claims 1 to 4,
Characterized in that the composition removes at least one gas selected from the group consisting of ammonia, sulfur dioxide, hydrogen sulfide, methyl mercaptan (CH ₃ SH), dimethyl sulfide [(CH ₃ ) ₂ S] A method for producing a composition for removing odor. 5. The method according to any one of claims 1 to 4,
Wherein the composition exhibits an antibacterial or bactericidal activity. The method according to claim 6,
Wherein the composition has antibacterial or bactericidal activity against at least one strain selected from the group consisting of E. coli, Salmonella, Pseudomonas, Clostridium, Staphylococcus, Enterococcus and Mycobacterium. &Lt; / RTI &gt;