KR20020040532A - Antioxidative drink excellent in inhibiting the side effects according to anticancer therapy and the method of manufacturing the same - Google Patents

Abstract

PURPOSE: A process of preparing an antioxidant drink having excellent antioxidant action by mixing low molecular weight red ginseng saponin and glutamate and an auxiliary component is provided. Whereby, the drink has an excellent effect on suppressing side effects caused by anticancer treatment and preventing various diseased caused by free radials. CONSTITUTION: This antioxidant drink is prepared by the subsequent processes: enzyme-treating saponin of red ginseng to make low molecular weight; mixing 1 to 15% by weight of saponin, 1 to 5% by weight of glumate having an active oxygen suppressing effect, an auxiliary component for improving antioxidant functionality and an additive and then absolutely dissolving in purified water; and filtering the mixed solution.

Description

Antioxidant drink excellent in inhibiting the side effects according to anticancer therapy and the method of manufacturing the same}

The present invention relates to an antioxidant drink having a side effect inhibitory effect according to anticancer therapy and a method for preparing the same, and more particularly, including low molecular weight red ginseng saponin having antioxidant activity and glutamate (Glumate, GMT) which is an active oxygen inhibitor. The present invention relates to an antioxidant beverage having a side effect inhibiting effect according to an anticancer therapy having excellent antioxidant activity, and a method for producing the same.

Free radicals are molecules that have one or more unpaired electrons and have the property of stabilizing electrons from other molecules. Free radicals are also very unstable, aggressive, and strong in oxidative power, with lifespans as short as thousands of seconds. Most of the free radicals are reactive oxygen species, which include hydroxyl radicals, superoxide radicals, singlet oxygen, and hydrogen peroxide (H ₂ O ₂ ). And alkoxyl radicals generated by lipid peroxidation.

As a common factor for generating such reactive oxygen species in the body, stress, alcohols, peroxides, drugs and the like are known.

On the other hand, there are more than tens of millions of cancer patients all over the world, and all of the anticancer therapies applied to them are known to have serious side effects, which are commonly known to be caused by free radicals.

Representative anti-cancer therapies and their side effects, first of all, radiation therapy, such as X-ray and radiation by irradiation with α, β, γ-rays, DNA degradation, cell death, loss of proliferative capacity, mutation, mutation of tumor cells, etc. Brings chemical changes. In addition, the generation of free radicals (OH, NO _2, etc.) and metabolic processes due to the combination of general or local anesthetics, hemostatic agents, anticoagulants, and analgesics during surgical therapy. Massive generation of free radicals due to oxidative stress such as transient anemia caused by bleeding can cause side effects such as fatigue, lowering white blood cell count, lowering immunity, and reducing platelets. Examples of chemotherapy agents include adriamycin, bleomycin, mitomycin, and cisplatin, which inhibit DNA synthesis, cell division, and cell membrane damage during long-term administration. And can cause reversible or irreversible side effects from DNA damage. As described above, most anti-cancer therapies all have serious side effects, and it is known that free radicals are commonly involved in the mechanism of action of the side effects.

To protect itself from damage caused by these free radicals, the body is equipped with a series of oxidative protection systems. Such protective systems include enzymes such as superoxide dismutase (SOD) and catalase, and α-tocopherol (vitamin E), β-carotene and ascorbic acid (ascorbic acid). fat-soluble and water-soluble antioxidants or radical scavengers such as acid, Vitamin C) and glutathione.

However, modern people are exposed to air pollution, unhealthy diet, UV exposure, stress, and harmful environment of illness, and it is difficult to eliminate oxidative stress caused by various radicals due to imbalance with the radical scavenging system in vivo. As a matter of fact, it is important to get enough antioxidant nutrients in order to function properly. In addition, the development of antioxidant foods that can prevent various diseases and side effects caused by free radicals by removing the active oxygen species generated in the body by itself.

On the other hand, ginseng is a plant that has been widely used as a representative nourishing tonic since ancient times, and is generally in the form of red ginseng prepared by heat-treating white ginseng or ginseng dried at room temperature, as it is grown and harvested at room temperature. It is used. Many researches on the components and efficacy of ginseng have been reported.The known effects of ginseng are anti-aging, anti-arteriosclerosis and hyperlipidemia, liver function enhancement, radiation disorder removal, immune enhancement, antithrombosis, brain hyperactivity. , Antistress, lowering blood sugar, lowering blood pressure or anticancer effect. In particular, red ginseng saponins, total saponins, are known to have antioxidant activity that reduces damage caused by free radicals or lipid peroxidation and blocks oxygen-induced damage in the biological system. It is still incomplete.

Accordingly, an object of the present invention is to provide an antioxidant beverage having a low side effect according to an anticancer therapy having excellent antioxidant activity, including low molecular weight red ginseng saponin and an active oxygen inhibitor glutmate having antioxidant activity.

Another object of the present invention is to provide a method for producing the antioxidant drink by combining a low molecular weight red ginseng saponin, glutate, an auxiliary component to improve antioxidant functionality and an adjuvant to add flavor or taste.

Another object of the present invention is to continuously drink the antioxidant drink according to the present invention to prevent various diseases and side effects resulting from free radicals by strengthening the overall immunity, and in particular, to suppress the anticancer therapy side effects, which are the main cause of free radicals To provide an antioxidant drink.

1 is a graph showing the free radical scavenging ability of the antioxidant beverage having the side effect inhibitory effect according to the anticancer therapy according to the present invention.

Figure 2 is a graph showing the role of glutmate (GMT) in the antioxidant capacity of the antioxidant beverage according to the present invention.

Antioxidant beverage having a side effect inhibitory effect according to the anticancer therapy according to the present invention for achieving the above object is characterized in that it comprises a low molecular weight red ginseng saponin having antioxidant activity and a glutate having an active oxygen inhibitory effect.

The red ginseng saponin is 1 to 15% by weight, glutamate is characterized in that it contains 1 to 6% by weight.

The method for preparing an antioxidant beverage having the side effect inhibitory effect according to the anticancer therapy according to the present invention comprises the steps of low molecular weight by enzyme treatment of red ginseng saponin having antioxidant activity, 1 to 15% by weight of the low molecular weighted red ginseng saponin 6 wt%, by mixing the auxiliary component to improve the antioxidant function and the additive to add aroma or taste to completely dissolved in purified water and the step of sterilizing and sealing the mixed solution to cool and repeat filtration.

Ginsenoside derivatives, ginseng's unique pharmacologically active saponins, contain sugars such as glucose, rhamnose, xylose or arabinose in the alcoholic hydroxyl groups of the protoparnasadiol and the protoparnasatriol, which are triterpenoids of the dimaran series. As the combined compounds, their molecular weight is so large that absorption into the body is not easy. Therefore, in the present invention, saponins were used in a low molecular weight by enzymatic treatment so as to increase the absorption of the polymer saponins in the body. At this time, the enzyme uses Deerland Cellulase 4000 and adds 0.01% to 0.1% by weight to red ginseng extract to lower the red ginseng saponin to facilitate the absorption in the body, thereby enhancing antioxidant activity.

Glumate used in the preparation method of antioxidant drink having the side effect suppression effect according to the anticancer therapy according to the present invention is extracted from microsaccharides and soybeans by alkaline fermentation, harmful oxygen inhibitor (superoxide dismutase (SOD) action, blood pressure inhibitory effect In addition, catalase and antistress are known to decompose hydrogen peroxide produced by the action of the antioxidant enzyme SOD1 into water and oxygen. In particular, active oxygen and free radicals such as OH ·, H ₂ O ₂ and ¹ O ₂ It is known to have an excellent effect on scavenging action. In the present invention, in order to increase the antioxidant activity, GMT-SOD, a glutamate 5-fold concentrate, which is an 'embryonic, soybean fermentation extract' invented by Dongyang Fermentation Co., Ltd., Japan, can be used. Most of them are produced by fermentation by alkalescent microorganisms as raw materials.

Supplementary ingredients and auxiliaries may be added to the low molecular weight red ginseng saponin and glutate. In this case, the auxiliary ingredient may be used as vitamin C, vitamin B ₁ , vitamin B ₂ , and the like as green tea extract and vitamins having antioxidant activity as an antioxidant function in addition to the main ingredient. Adjuvants add flavors or flavors, for example, herbal flavors, oligosaccharides, sodium citrate, CMC and the like can be used.

The amount of red ginseng saponin in the step of mixing these ingredients to make an antioxidant drink is preferably used in the range of 1 to 15% by weight, glutamate 1 to 6% by weight in the total beverage composition. If the amount of red ginseng saponin and glutate is less than 1% by weight, it does not have a definite effect on the antioxidant activity and the antioxidant activity increases as the amount increases. However, when the amount of red ginseng saponin exceeds 15% by weight, it is not suitable for drinking due to the bitter taste peculiar to red ginseng, and glutmate is an expensive product and shows excellent antioxidant activity even under 6% by weight. Preferred component ratios of the antioxidant beverage according to the present invention are shown in Table 1 below.

Table 1 Composition of Antioxidant Beverage

In the step of sterilizing, sealing and cooling the solution in which the above components are mixed, the filter is repeatedly filtered four or more times using a 10 μ microfilter to remove impurities that may remain in the beverage mixture.

Hereinafter, the method and effect of preparing the beverage according to the present invention will be described in more detail with reference to Examples, but the scope of the present invention is not limited to the following Examples.

Example 1

500 g of red ginseng was added to 1 L of ethanol, and the method of extracting and filtering by ultrasonic apparatus at 70 ° C. for 5 hours was repeated three times. The red ginseng extract of about 2.5L was depressurized to 1.5L with a decompression concentrator, and 0.1% of Deerland Cellulase 4000 was added to the red ginseng extract. After 12 hours, the enzyme was inactivated by heat treatment for 10 minutes in a 100 ° C. constant temperature water bath, followed by centrifugation at 3,500 × g for 20 minutes, and the supernatant was taken to prepare low molecular weight red ginseng saponin.

Example 2

The low molecular weight red ginseng saponin, glutmate, green tea extract, oligosaccharide, vitamin C, vitamin B ₁ hydrochloride, vitamin B ₂ , herbal flavor, sodium citrate, and CMC prepared in Example 1 were mixed in the ratio of Table 2 below. After dissolving completely at 80 ° C., the solution was sterilized at 95 ° C. for 30 minutes, sealed, and cooled for 18 hours.

Table 2 Composition table of antioxidant drink according to Example 2

The cooled solution was repeatedly filtered several times with a 10 占 micro filter to obtain a dark brown clear liquid, which was filled and sealed by quantitation. The liquid was then sterilized and packaged at 95 to 100 ° C. for 30 minutes to prepare a finished product of this product.

Example 3

Except that GMT-SOD was removed from the low molecular weight red ginseng saponin prepared in Example 1, red ginseng saponin was prepared by mixing the same components and compositions as in Example 2 and removing the glutate in the same manner.

Stability test was performed according to the sensory test and food standards test method for a beverage containing low molecular weight red ginseng saponin and glutate prepared according to an embodiment of the present invention. The test method is based on the packaged product and five sensory inspectors inspecting the color, flavor, and odor of the product stored in an electric thermostat (40 ℃) for 60 days. It is a product with little change, which can give consumers satisfaction, and it is a product with merchandise, and the result is shown in Table 3 below.

Table 3

As a result of the test of Table 3, the beverage prepared according to the embodiment of the present invention has been proved its stability against the appearance and general bacteria and E. coli over 18 months.

Antioxidant activity, ie free radical scavenging activity, was measured as follows for the beverages prepared according to the embodiment of the present invention. The method used was performed by the 1,1-Diphenyl-2-Pycryl-Hydrazyl (DPPH) method. DPPH is a relatively stable free radical which, when present in the radical state, exhibits maximum absorbance at 517 nm and loses absorbance when erased. DPPH was used by Sigma, USA, and 0.006 g of 50 ml of ethanol was dissolved, and 50 ml of water was added to make a 0.006% solution. After taking 3 ml of this DPPH solution into a test tube, the beverage according to Example 2 of the present invention was taken from 0.05 ml to 0.55 ml for each concentration, mixed well, and left at room temperature for 15 minutes, and the absorbance at 517 nm was measured. At this time, the control was used to put water instead of the beverage according to the present invention. The absorbance of the sample is compared with that of the control group and is shown in FIG. 1.

1 shows free radical scavenging ability, that is, antioxidant capacity, of the beverage according to the present invention. The free radical scavenging ability provides an electron to the oxidative free radicals involved in the chain reaction of lipid peroxide, thereby stopping the chain reaction and inhibiting oxidation, thus becoming a measure of antioxidant capacity. Therefore, as can be seen in Figure 1, the free radical scavenging ability of the beverage added glutamate to red ginseng saponin according to the present invention showed a 2.5 to 3.0 times better antioxidant capacity than a control without a sample It can be seen.

In order to determine the role of glutmate in the antioxidant capacity of the beverage according to an embodiment of the present invention for the sample prepared in Example 3, in which the beverage and glutate prepared in Example 2 including red ginseng saponin and glutate were removed, The same experiment was conducted. Take 0.2 ml of the beverage according to the present invention as a sample, add 2.0 ml of 2% sodium carbonate (Na ₂ CO ₃ ), mix well for 2 minutes, add 0.2 ml (50%) of Folin-ciocalpeu's reagent as a color development reagent, and leave it at room temperature. After measuring the absorbance at 750nm to calculate the amount of total phenolic acid (total phenolic acid) that is a component showing antioxidant activity. At this time, samples were taken at each concentration from 0.2ml to 17ml and chlorogenic acid was used as a standard sample. As a control, the samples prepared in Example 3 were taken at the same concentration, and the absorbance was measured. The results are shown in FIG.

Figure 2 shows the role of glutmate in the antioxidant capacity of the beverage according to the present invention was measured for the amount of total phenolic acid which is a component showing the antioxidant activity for the beverage and the beverage without glutamate according to the present invention. As can be seen in Figure 2, the beverage according to the present invention is a total phenolic acid amount of 16.94 mg / ㎖ while the total amount of phenolic acid of the SOD-removed sample shows 1.99 mg / ㎖ not only red ginseng saponin in antioxidant capacity The role of the mate is also important. That is, the present invention has developed a beverage that exhibits excellent antioxidant capacity, including red ginseng saponin and glutate.

Antioxidant beverages according to the present invention are prepared by including red ginseng saponin with improved antioxidant capacity and red ginseng saponin and glutamate, which is an antioxidant substance, to show excellent free radical scavenging ability. It is effective in inhibiting the effects of various diseases caused by free radicals. Furthermore, by continuously drinking the antioxidant drink according to the present invention there is an effect that can enhance the overall immunity.

Although the present invention has been described in detail only in the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the technical spirit of the present invention.

Claims (3)

An antioxidant beverage having a side effect inhibitory effect according to an anticancer therapy comprising low molecular weight red ginseng saponin and a glutate having an active oxygen inhibitory effect. The method of claim 1, The low molecular weight red ginseng saponin is 1 to 15% by weight, glutamate containing 1 to 6% by weight of the antioxidant drink having a side effect inhibitory effect according to the anticancer therapy. Low molecular weight by enzyme treatment of saponin of red ginseng; 1 to 5% by weight of the low molecular weight saponin, glutamate 1 to 5% by weight of the active oxygen inhibitory effect, an auxiliary component to improve the antioxidant function and aroma or flavoring adjuvant is completely dissolved in purified water; And Sterilizing and sealing the mixed solution to cool and repeat filtration; Method for producing an antioxidant beverage having a side effect inhibitory effect according to the anticancer therapy.