KR20020014453A - Ultraviolet-protecting cosmetic composition containing Saxifragaceae extracts - Google Patents

Abstract

PURPOSE: Provided is a skin care cosmetic composition for blocking UV containing Saxifragaceae extract. It activates DNA repairing enzyme system in vivo thus inhibits the mutation of a skin cell caused by DNA damage by UV and has a UV blocking effect. CONSTITUTION: The composition comprises 0.1-60, preferably 0.1-25 wt.% of Saxifragaceae extract having a bio-anti-mutagen, to the total weight of the composition, wherein the extract comprises tannin derivative and terpin essential oil as a bio-anti-mutagen, and is preferably extracted by the steps of: finely cutting 10kg of Saxifragaceae; adding 30 volume % of polyol; then extracting and filtering it.

Description

Ultraviolet-protecting cosmetic composition containing Saxifragaceae extracts}

The present invention relates to a UV protective skin cosmetic composition containing a sublingual extract (雪 x; Saxifragaceae ), more specifically, in vivo DNA repair enzyme system by containing a sublingual extract of 0.1 to 60% by weight of the total composition It relates to a skin cosmetic composition having a UV protective effect by inhibiting skin cell mutations caused by DNA damage caused by ultraviolet rays.

It is well known that ultraviolet radiation is harmful to the skin. In general, much research has been done on the mechanism by which ultraviolet light affects melanin formation and active enzyme and protein denaturation in the skin and causes aging (photoaging). On the other hand, studies on DNA damage and skin cell mutations, which are the most serious effects of ultraviolet rays on the skin, have not been conducted properly, so the method for protecting against ultraviolet rays has been limited to using a composition containing a sunscreen. However, as the research on the cause of skin cancer caused by DNA damage caused by ultraviolet light has been developed, interest in not only the effect of DNA damage on photoaging but also on the increase of skin cancer is rapidly increasing.

The mechanism by which ultraviolet light causes DNA damage in the skin can be summarized as ultraviolet light directly damages DNA or indirectly activates enzymes. This DNA damage means that pyrimidine, one of the DNA bases, is transformed into an oligomer or 6-4 condensate, or the DNA is cleaved by ultraviolet energy. At this time, if DNA damage is very large and the body fails to recover its own DNA repair (repair) system, a DNA sequence mutation occurs. These mutations cause abnormal protein synthesis or cell necrosis. Thus, DNA mutations in skin cells are closely associated with photoaging and skin cancer.

There are two types of substances that can protect against cell-induced mutagens, including UV rays. One is desmutagen, which acts chemically, enzymatically or physically directly on cell-induced mutagens and inactivates them. Let's do it. When ultraviolet light is a cell mutagenesis factor, melanin, superoxide dismutase (SOD), a sunscreen, etc. belong to this. The other is bio-anti-mutagen, which acts on damaged cells to inhibit mutations, which is believed to activate DNA repair enzyme systems to promote DNA repair. Bio-anti-mutagen can be applied to a variety of cell mutation triggers than desmutagen.

In order to prevent DNA mutations caused by UV rays, research on cosmetic compositions containing bio-anti-mutagen has begun. In particular, research and development on cosmetic compositions using natural raw materials are actively conducted to meet the needs of consumers. It is becoming a trend.

On the other hand, the tongue is called the tiger ear in China, has been used for a long time mainly for the treatment of contact dermatitis, burns, frostbite, etc., and has also been used as an anti-inflammatory agent.

Accordingly, the present inventors have developed an ultraviolet protective cosmetic composition different from the existing sunscreen containing composition, which has an effect of inhibiting skin cell variation caused by ultraviolet rays, caused by photoaging and DNA damage causing skin cancer. Continuous studies were conducted for this purpose. As a result, the present inventors have found a surprising fact that the composition of the present invention prepared by appropriately blending sublingual extracts exhibits significantly improved DNA damage suppressing effect and damaged DNA repair effect, and completed the present invention.

Accordingly, an object of the present invention is to contain a sublingual extract, thereby activating the DNA repair enzyme system in vivo to suppress skin cell mutations caused by DNA damage caused by ultraviolet light, ultimately to inhibit photoaging and skin cancer It is to provide a protective skin cosmetic composition.

The present invention provides a UV protective skin cosmetic composition containing a sublingual extract of 0.1 to 60% by weight based on the total weight of the composition. The composition according to the present invention can activate the DNA repair enzyme system in vivo to inhibit skin cell mutation caused by DNA damage caused by ultraviolet light. In the present composition, the sublingual extract preferably contains tannin derivatives and terpin essential oils, and it is preferable that the sublingual extract is obtained by extracting fine sublingually with a polyol. Further, it is more preferable to contain 0.1 to 25% by weight of sublingual extract relative to the total composition weight.

Hereinafter, the present invention will be described in detail.

In the composition of the present invention, the sublingual extract is extracted from sublingually purified tannin derivatives (e.g., proanthocyanidins) and terpin essence oils (e.g., leaf alcohols, camphene, Linalool, borneol, and the like). In other words, an appropriate extraction solvent, preferably a polyol, is added to an appropriate amount, preferably 30% by volume, and the resultant is extracted and filtered under a subdivided tongue to obtain a final extract. In the present invention, as the sublingual extract, for example, commercially available Yukinoshita Liquid-MB (manufactured by Ichimaru Pharcos Co., Ltd.) can be used.

In the present invention, the sublingual extract acts to inhibit DNA damage by ultraviolet rays. More specifically, the damage of DNA genetic information by ultraviolet light causes a change in DNA sequence or necrosis of cells. As soon as the DNA sequence changes, a mutation occurs. In order to prevent such changes in DNA genetic information and cell necrosis, a DNA repair enzyme system exists in the cell itself. The substance that enhances the activity of this system is called bio-anti-mutagen. The composition of the present invention can exert a skin protective effect against ultraviolet rays by reducing damage such as DNA damage by ultraviolet rays by the action of the sublingual extract on the skin.

In the present invention, the effect of bio-anti-mutagen that inhibits DNA damage by ultraviolet rays is evaluated by measuring the degree of suppression of necrosis of cells and the degree of inhibition of cell variation that occur after ultraviolet irradiation. More specifically, the degree of inhibition of cell necrosis is measured by the survival rate of E. Coli after UV irradiation, and the degree of inhibition of cell mutation is measured by the rate of mutant cell generation. The reason for using E. coli is that even though DNA repair enzymes are different in E. coli and mammals, the effects of substances that promote DNA repair, such as bio-anti-mutagen, have been reported to be the same. At this time, one species of E. coli is converted and used so as to be suitable for detection of UV-induced mutations. This E. coli species does not produce tryptophan, an essential amino acid, without UV irradiation, so it does not grow on tryptophan-deficient media, but E. coli mutated by UV produces tryptophan and also on tryptophan-deficient media. In other words, E. coli, which requires tryptophan for growth, uses mutations that can be produced without tryptophan by causing mutations to produce tryptophan by ultraviolet rays, thereby measuring the incidence of cell mutations in E. coli. The effect of inhibiting cell mutation is evaluated as the extent to which cell incidence decreases and the bactericidal effect of ultraviolet light (DNA damage factor) decreases, thereby increasing cell survival rate. In the present invention, tryptophan-dependent E. coli is tested in three groups, first, the group irradiating only ultraviolet light, second, the group to add sublingual extract after ultraviolet irradiation, third, the group not to irradiate ultraviolet rays and do not add sublingual extract to be. Determine the number of cell variants in E. coli and normal E. coli in each group.

On the other hand, the amount of the sublingual extract, which is a characteristic component of the composition of the present invention can be appropriately adjusted according to the type of cosmetic composition, but preferably 0.1 to 60% by weight, more preferably 0.1 to 25% by weight relative to the total cosmetic composition %, Most preferably 25% by weight. If sublingual extract is less than 0.1% by weight of the DNA damage inhibitory effect is minimal, even if it is blended in more than 60% by weight, especially more than 25% by weight because the effect is not so increased.

In addition to the sublingual extract, the cosmetic composition of the present invention may be formulated with an appropriate amount of an oily base, a surfactant, a sunscreen, an antioxidant, a preservative, a perfume, a dye, a pH adjuster, and the like within a range that does not impair the effects of the present invention.

EXAMPLE

Hereinafter, the present invention will be described in more detail with reference to Examples, Comparative Examples and Experimental Examples, but the scope of the present invention is not limited to the following Examples. The sublingual extract used in the following examples used the following products.

(Sublingual extract)

1. Extraction Method

(1) Cut 10 kg of sublingually.

(2) Polyol is added to 30 vol%.

(3) Extraction and filtration obtain final extract.

2. Company name: Ichimaru Pharcos Co., Ltd.

3. Product Name: Yukinoshita Liquid-MB

4. Composition: Tannin derivatives, terpines (leaf alcohol, camphene, linalool, borneol)

Examples 1-16 and Comparative Examples Preparation of Cream

To the composition (except sublingual extract) shown in Table 1, the oil phase component and the water phase component were completely dissolved, respectively, and emulsified in the emulsifying unit and cooled.

Ingredient Name content Sublingual extract The amount listed in Table 2 White sugar fatty acid ester 1.0 Methylparaben 0.15 Imidazolidinylurea 0.2 glycerin 7.0 Carbomer 0.15 Sorbitan monostearate 1.8 Decaglyceryl Myristate 1.2 Glyceryl Monostearate 2.0 Cetostearyl alcohol 2.0 Beeswax 1.0 Ceresin 1.0 Propylparaben 0.1 Liquid paraffin 3.0 Capric / Caprylic Triglycerides 17.0 Squalane 5.0 Methylpolysiloxane 0.5 Purified water Up to 100

After cooling to 40 ° C., sublingual extract was added to the content shown in Table 2 below to prepare a cream.

Example / Comparative Example Sublingual Extract Example / Comparative Example Sublingual Extract Content Comparative example - Example 9 20.0 Example 1 0.1 Example 10 25.0 Example 2 1.0 Example 11 30.0 Example 3 2.0 Example 12 35.0 Example 4 3.0 Example 13 40.0 Example 5 5.0 Example 14 45.0 Example 6 7.0 Example 15 50.0 Example 7 10.0 Example 16 55.0 Example 8 15.0

Experimental Example Inhibition of DNA Damage by UV Light

Test Sample: Yukinoshita Liquid-MB (30 vol% Polyol Extract in Sublingual)

Strain: Escherichia coli B / r W2 tryptophan (IFO 14195)

badge:

B-2 Liquid Medium- A medium for strain propagation consisting of bovine extracts and polypeptides

B-2 Agar Medium-Medium added agar to B-2 liquid medium

SEM agar medium-medium supplemented with 0.008% nutrient solution to minimal glucose agar to prevent the growth of tryptophan-dependent strains

Experimental method:

The medium was removed by centrifugation from cells cultured for 15 hours at 37 ° C. in B-2 liquid medium, and then suspended in 100 mM sodium phosphate buffer. The turbidity of the suspension was measured by optical density and diluted to 200-300 x 503 / 0.1 ml cell concentration to use as cell suspension. 20 ml of the cell suspension was taken into a sterile container, and then 48 J / m 2 ultraviolet light was irradiated to the container. 0.1 ml of the test sample and 0.5 ml of sodium phosphate were added to 0.1 ml of the cell suspension irradiated with ultraviolet light. The cell suspension was sufficiently shaken at 37 ° C. for 30 minutes and then divided into two groups. One group was incubated with gentle agar added to B-2 agar medium to dilute to 503 cell density and measure cell number. After 24 hours at 37 ° C., the number of cells was measured and considered as viable cell number. The other group was cultured in SEM medium with gentle agar and after 48 hours at 37 ° C., the number of cells was measured and considered as the mutant cell number.

To measure the effect of inhibiting cell mutations:

Inhibition of cell mutation was assessed by the survival and mutation incidence of UV-irradiated cells. The viable cell number before UV irradiation was calculated as 100% survival rate, and the incidence of mutation was calculated as the viable cell number and the mutant cell number.

In three groups (① UV irradiation group, ② UV irradiation + test sample treatment group, ③ control group), cell survival rates measured in B-2 medium were S1, S2, and S3, respectively. T1, T2, and T3.

Survival Rate (%) = S2 / S3 × 100 (%)

Frequency of cell mutations = (T1-T3) / S1 = M1

Incidence of cell mutation = M2 / M1 × 100 (%)

Experiment result:

As shown in Table 3 below, the incidence of cell mutation due to ultraviolet rays decreased proportionally with the content of Yukinoshita Liquid MB, and the cell viability also increased with the content of Yukinoshita Liquid MB.

Growth test of tryptophan-dependent E. coli An experimental sample UV Survival rate (%) Variation frequency % Variation % Inhibition 30% 1,3-BG X 100 - - - 30% 1,3-BG O 31.0 4.72 100 - Comparative example O 30.9 4.70 100 - Example 1 O 9.2 5.55 99.5 0.5 Example 2 O 47.9 3.20 90.4 9.6 Example 3 O 49.1 2.93 85.8 14.2 Example 4 O 49.7 2.74 72.8 27.2 Example 5 O 51.0 2.54 63.7 36.3 Example 6 O 51.1 2.20 59.3 40.7 Example 7 O 52.0 1.89 47.0 53.0 Example 8 O 52.5 1.42 35.0 65.0 Example 9 O 53.3 1.27 26.0 74.0 Example 10 O 55.5 1.14 24.2 75.8 Example 11 O 55.5 1.12 23.7 76.3 Example 12 O 55.5 1.10 22.5 77.5 Example 13 O 55.5 1.05 21.9 78.1 Example 14 O 55.5 1.02 21.5 78.5 Example 15 O 55.5 0.98 20.8 79.2 Example 16 O 55.5 0.95 20.5 79.5

* O: No UV irradiation, X: No UV irradiation

As shown in Table 3, Yukinoshita Liquid MB is one of the bio-anti-mutagen to reduce the toxicity of harmful ultraviolet rays, when used at 25% by weight (Example 10) showed the most effective effect.

The cosmetic composition according to the present invention exhibits an excellent protective effect against skin cell DNA attack by ultraviolet rays by containing 0.1 to 60% by weight of sublingual extract.

Claims (5)

UV-protective skin cosmetic composition containing 0.1 to 60% by weight of sublingual ( Saxifragaceae ) extract based on the total weight of the composition. The composition of claim 1, wherein the composition activates the DNA repair enzyme system in vivo to inhibit skin cell variation caused by UV damage-induced DNA damage. The composition of claim 1 or 2, wherein the sublingual extract contains tannin derivatives and terpine essence oils. The composition according to claim 1 or 2, wherein the sublingual extract is obtained by extracting the subcutaneous sublingually with a polyol. The composition according to claim 1 or 2, which contains 0.1 to 25% by weight of sublingual extract, based on the total weight of the composition.