KR20000036753A - Microorganisms for Fermenting Organic Waste - Google Patents

Abstract

PURPOSE: Microorganism composite (KCCM 10165) for the fermentation organic waste having a large amount of hydrocarbone and salts is provided which degrades organic waste for the purpose of making organic animal feed-stuff or fertilizer. CONSTITUTION: 1g of food waste is vortexed for 10 minutes, diluted with 10 ml of saline and smeared on MRS agar plate, nutrient agar plate, potato dextrose agar plate or YPD agar plate and cultured at 30°C for 2-3 days to obtain 80 colonies of bacillus, yeast, lactobacillus and fungi. 16 strains are selected based on the growth rate and the strong degradability of protein, starch, cellulose. 6 strains are selected finally based on salts and thermo tolerance. The six strain, Bacillus sp. HE-05, Bacillus sp. HE-06, Bacillus sp. HE-07, Bacillus sp. HE-08, Bacillus sp. HE-09, Bacillus sp. HE-10, Torulopsis sp. HE-11, Torulopsis sp. HE-11 are mixed with three strains, Saccharomyces sp. HE-04, Aspergillus sp. HE-04, Rhizopus sp. HE-02, Lactobacillus sp. HE03. The composite is suspended in liquid for the distribution or preservation or mixed with powder of rice bran, wheat bran, corn bran, barley bran, corn, bread or biscuit.

Description

Microorganisms for Fermenting Organic Wastes {Microorganisms for Fermenting Organic Waste}

The present invention relates to a microbial complex used for fermentation of organic waste, and more particularly to a microbial complex for organic waste fermentation, consisting of Bacillus and yeast strains isolated from food waste, and industrial filamentous fungi and lactic acid bacteria. will be.

With the recent development of the industry, urban waste and industrial waste are becoming a global problem, and organic waste such as food waste, food processing residue, and food waste emitted from food factories, restaurants, and households is 30% of the total urban waste throughput. Is reaching. Such organic waste causes aesthetic disgust, becomes a hotbed of various harmful insects such as flies, smells badly during decay, and decay water (leached water) contaminates soil and water, causing environmental pollution and disturbing natural ecosystems. It is a factor.

Conventionally, organic wastes have been treated by landfill or incineration, but these treatment methods have reached their limits due to saturation of landfills, reduction of sites, diversification of incinerators, and primary and secondary environmental pollution. It is becoming.

Such organic wastes can be utilized as high quality organic feed or organic fertilizers if properly fermented. For this purpose, good microorganisms or groups of microorganisms suitable for the characteristics of organic wastes are essential, but conventionally microorganisms for this purpose have been isolated from soil or introduced from abroad. In other words, the microorganisms separated from the soil show excellent characteristics in the soil environment, but they do not show smooth fermentation performance under organic wastes with completely different natural substances.The microorganisms introduced from abroad are Korean organic wastes with high carbohydrate content and high salt content. The result is not satisfactory in terms of cost and efficacy because it is not suitable.

The present invention is to solve the disadvantages of the microorganisms or microbial preparation for organic waste fermentation as described above, an object of the present invention is to provide a microbial complex for organic waste fermentation suitable for Korean characteristics.

In order to achieve the above object, the present invention, Bacillus HE-05 (Bacillus sp. HE-05), Bacillus HE-06 (Bacillus sp. HE-06), Bacillus HE-07 (Bacillus sp. HE-07) , Bacillus HE-08 (Bacillus sp. HE-08), Bacillus HE-09 (Bacillus sp. HE-09), Bacillus HE-10 (Bacillus sp. HE-10), Torulopsis HE-11 (Torulopsis sp. HE-11), Torulopsis HE-12 (Torulopsis sp. HE-12), Saccharomyces HE-04, Aspergillus HE-01 (Aspergillus sp. HE-01) It provides a microbial complex for fermentation of organic waste consisting of, Lyzopus HE-02 (Rhizopus sp. HE-02), Lactobacillus HE-3 (Lactobacillus sp. HE-03).

The microbial complex according to the present invention may be suspended in a liquid state and stored and distributed as necessary, and mixed and stored in any one or two or more powders selected from rice bran, bran, corn barley, barley bark, corn, bread, and confectionery, It is also possible to distribute.

Hereinafter, the present invention will be described in more detail.

Isolation and Identification of Microorganisms

In the present invention, each strain contained in the microbial complex was distributed from other domestic companies, or separated and identified as follows.

Various food wastes were collected from various parts of Korea and used as samples. About 1 g of the sample was shaken for 10 minutes in 10 ml of saline, and then diluted appropriately. MRS agar medium (Difco Lab, MRS medium) and nutrient agar medium (Difco Lab) for the growth of Bacillus, yeast, Lactobacillus and filamentous strains, respectively , NA medium), potato dextrose agar medium ((Difco Lab, PDA medium), YPD medium ((Difco Lab) and plated in 30 ℃ incubated for 2-3 days in a colony of 80 strain colonies were isolated by primary pure.

Using the same medium as described above, 16 strains having spore forming ability, fibrin resolution, starch resolution, protein resolution, and excellent growth ability were isolated, and then, among these 16 strains, the strains showing flame resistance and thermophilic properties were isolated. 6 was finally isolated and the strains were named HE-05, HE-06, HE-07, HE-08, HE-09, and HE-10, respectively.

Separately, three yeast strains having excellent sugar availability were isolated from primary pure strains and named HE-11, HE-12, and HE-04, respectively.

The morphological and physiological characteristics of the microorganisms isolated by the above method were investigated and shown in the following tables.

Cell Forms of Flame-Resistant / Heat Resistant Strains HE-05 HE-06 HE-07 HE-08 HE-09 HE-10 Cell form Stick Stick Stick Stick Stick Stick Gram dyeing positivity positivity positivity positivity positivity positivity

Physiological Characteristics of Flame-Resistant / thermophilic Strains Strain HE05 HE06 HE07 HE08 HE09 HE10 Tetrazolium red - - - + - + Amig + - - - - - N-acetyl-D-glucosamine + + + + - + Amylopectin - + + + - + Potassium Thiocyanate + + + + + + 7% sodium chloride - + + + + + Mandelic acid + + + + + + Oleandomycin - + - + - - Acetic acid chloride - + + + - + Polyamidohydrostriptin + + + + + + Nalidi Foods - - + + - + Escalin + + + + + +

Sugar Availability of Flame Tolerant / thermophilic Strains Strain HE05 HE06 HE07 HE08 HE09 HE010 Sucrose + + + + + + Catetose + - - - - - Glucose + + + + + + Inositol + + + + + + Galactose - - - + + - Arabinose - + + + + + Xylose - - - + + + Mannitol + + + + + + Raffinose - + + + - + Salinity + + + + - - Inulin - + + + + + Ribose - - - - + - maltose - + + + + + Trehalos + + + + + + Palatinose + + + + + + Sorbitol - - - + - + Arabitol - - - + - -

Physiological features of yeast Strain HE11 HE12 2-keto-D-gluconate - - α-methyl-D-glucoside - - N-acetyl-D-glucosamine - +

Sugar availability of yeast Strain HE11 HE12 Glucose + + Glycerol - + L-arabinose - - D-xylose - - Adonitol - - Zyritol - - Galactose - - Inositol - - Sorbitol - - Cellobios - - Lactose - - maltose - - Sucrose - - Trehalose - - Melezitose - - Raffinose - -

As a result, all of the salt tolerant / thermophilic strains were found to be in the genus Bacillus, and the yeast strains were Torulopsis sp., Torulopsis sp., And Saccharomyces sp. .) Turned out to be cheating.

The microbial complex of the present invention is an industrial filamentous bacterium known as Aspergillus oryzae, Rhizopus oryzae, and Lactobacillus acidophilus (KCTC 2182), which are known to be fast growing and excellent in carbohydrate resolution. The strains are mixed. The Aspergillus was purchased from Suwon Fermented Food Research Institute, Lysops was assigned to Co., Ltd., and Lactobacillus was purchased from Gene Bank. The present inventors have named the three filamentous strains HE-01, HE-02 and HE-03 for convenience.

As described above, the microbial complex according to the present invention was deposited on June 9, 1999 by KCCM-10165, the Korean spawn association.

Preparation of Microbial Complex

Each strain was cultured independently under the culture medium and culture conditions shown in Table 6.

Culture medium Culture condition Final concentration Bacillus genus Soybean 3% Molasses 0.7% 24 hours incubation at 180 rpm in a 35 ° C Shaking Incubator 1 × 10 ¹⁰ or more each leaven Soybean 3% Molasses 3% Incubate 24 hours at 180 rpm in a 30 ° C Shaking Incubator 1 × 10 ⁹ or more each Lactobacillus Soybean 3% Molasses 2% Incubate 24 hours in a 37 ℃ incubator 1 × 10 ⁹ or more each Aspergillus / Raizovs Wheat bran 3% agar 1.5% 48-72 hours incubation in 30 ℃ / 35 ℃ incubator 1 × 10 ⁸ or more (100% spores)

The cultured cultures were mixed in a ratio of 40% Bacillus, yeast 25%, lactic acid bacteria 15%, filamentous bacteria 20% by the volume ratio of the culture medium, and 0.1% potassium sorbate (K-Sorbate) was added as a stabilizer to prepare the microbial complex of the present invention. Prepared. At this time, the strain belonging to each strain was mixed in the same ratio with respect to the%.

Application example 1

60% by weight of food waste (remnants) collected at a traditional Korean restaurant and 40% of rice bran in order to match the water content, add 100ml of the microbial complex according to the present invention per ton of the mixture and evenly mix in a 30 ℃ stirrer Livestock feed was prepared by fermentation for 17 hours with stirring every 6 hours. The nutritional components and the amount of residual microorganisms of the prepared feeds are analyzed and shown in Tables 7 and 8 below.

Analysis item Analysis (%) moisture 9.55 Crude protein 16.98 Crude fat 8.61 Crude fiber 5.64 View minutes 6.74

Microorganisms per gram of feed Remarks Bacillus genus 6.7 × 10 ⁸ B. licheniformis, B. subtilis leaven 2.5 × 10 ⁷ Lactobacillus 3 × 10 ⁷ Filamentous fungus 5 × 10 ⁶ Escherichia coli voice

Application example 2

Pig group breeding with feed fermented by adding the microbial complex according to the present invention in the application example 1, and pig group breeding with feed residue + rice bran without addition of the microbial complex were treated and untreated, respectively, for 100 days. Breeding. As can be seen in Table 9, the treatment group fed the feed with the microbial complex according to the present invention gained an improvement of 10.7% in weight gain and 33.8% in feed efficiency compared to the non-treatment. In the treatment group, mortality rate, respiratory disease, diarrheal disease, etc. occurred less frequently than the untreated group.

Test head Test period Initial average weight Average weight Average weight gain Feed rate Treatment 35 100 days 24.3 Kg / Two 99.8 Kg / Two 75.5 Kg / Two 2.54 Untreated 31 100 days 24.5 Kg / Two 92.7 Kg / Two 68.2 Kg / Two 3.40

Test place: Seongho Nongsan, Chungnam

When the microbial complex of the present invention is a mixture of strains isolated from domestic organic wastes and ferments organic wastes including food wastes by using the same, the food wastes can be used for livestock feed, and the feed thus prepared increases pig growth rate. Effect. In addition, when treating the organic waste using the microbial complex according to the present invention, it can be used as an organic feed fast decomposition and no harmful ingredients.

Claims (3)

Bacillus HE-05 (Bacillus sp. HE-05), Bacillus HE-06 (Bacillus sp. HE-06), Bacillus HE-07 (Bacillus sp. HE-07), Bacillus HE-08 (Bacillus sp. HE-08 ), Bacillus HE-09 (Bacillus sp. HE-09), Bacillus HE-10 (Bacillus sp. HE-10), Torulopsis HE-11, Torulopsis HE-12 ( Torulopsis sp.HE-12), Saccharomyces sp.HE-04, Aspergillus sp-01, -01 Lysperp HE-02 (Rhizopus sp.HE -02), Lactobacillus HE-3 (Lactobacillus sp. HE-03) microbial complex for fermentation of organic waste, characterized in that consisting of (KCCM-10165). The method of claim 1, A microbial complex for organic waste fermentation, which is suspended in a liquid medium. The method of claim 1, Microbial complex for organic waste fermentation, characterized in that it is mixed with any one or two or more powders selected from rice bran, bran, corn bark, barley bark, corn, bread, confectionery.