KR19980025198A - Method of Artificial Cultivation of Cordycepsmilitaris by Using Jam - Google Patents

Abstract

The present invention relates to a method of artificially growing Cordyceps militaris, which is known to be excellent in various effects such as anticancer and immunity enhancement, but is not useful to humans because it is extremely difficult to collect in nature. After sprinkling spores of Incodiceps Militaris on the epidermis of the fifth-larvae larvae of Gajam, fruiting bodies are formed in the pupa that forms mycelium.

Description

Artificial Cultivation Method of Chrysalis Cordyceps Sinensis (Corpdycesps militaris) Using

The present invention relates to a method of artificial cultivation of chrysalis of the pupa larvae (번 夏 草) by using the scavenger, more specifically, after inoculating the larvae of live larvae (Cordyceps militaris) to induce the infection, the worms infected with the bacteria The present invention relates to a method of artificially growing cordyceps militaris, which is a kind of Cordyceps sinensis, by forming fruiting bodies in.

Cordyceps sinensis is a kind of medicinal mushroom from which fungus Cordyceps sinus is infected with insects and is known to grow native to China, Nepal, Japan, South America and Korea.

The path of infection of cordyceps relies mainly on transdermal infection caused by conidia scattering and attaching to the insect epidermis.When spores adhere to the epidermis and meet conditions such as temperature and humidity, they germinate and penetrate the epidermis of the insect. The growth and development of the mycelia in the mycelia, after the death of the mycelia of the body will form fruiting bodies on the epidermis of host insects. Fruiting bodies form 1.5 ~ 10cm sized filaments, clubs, cylinders, etc., which are composed of diseased and distal ends, and have various colors such as white, black, red, and brown.

Cordyceps sinensis, which has the same physiological and ecological characteristics as above, has been the subject of much interest and research today because of its pharmacological effects on various diseases.

For example, in China, Cordyceps sinensis has long been widely known as a elixir of firemen and has been favored as a valuable medicine for protecting the lungs and strengthening the kidneys. Several species of Cordyceps sinensis, including Azibong bongchoong, have been reported to be effective in a wide range of diseases. In addition, in the case of Japan, records of Cordyceps sinensis were transmitted to the herbaceous book in 1801, and were sold as a special medicine for lung disease and pleurisy at that time. In addition, according to recent research results of China, Japan, etc., when Cordyceps sinensis extract, which contains a component that enhances immune function, is administered to the human body, it promotes cellular immunity and humoral immunity, thereby reinforcing immunity functions and various tumors and viruses. It has been shown to increase the resistance to infection. In a clinical trial in Switzerland, a drug addict also succeeded in returning to a normal normal by taking two weeks of treatment to eliminate the side effects of the drug and to stop temptation. In addition, Cordyceps sinensis has a very wide field of use because it is effective in relieving fatigue quickly when there is a lot of physical strength due to severe exercise and also in relieving stress of those who work mentally.

There are about 300 species of Cordyceps sinensis that show various effects, and a large number of species are known to show different efficacies. Therefore, they are expected to be used as raw materials for natural medicines, but they are larger in size than other mushrooms or medicinal plants. Because of its small size and low distribution density, it is difficult to find and collect in outdoor mountains, so it is not only able to secure the mass demand but also the absolute amount necessary for various studies such as drug search and clinical trials.

Therefore, active research on the artificial cultivation method of cordyceps is underway by understanding the types of caterpillars, host insects, path of infection, fruiting body formation conditions, etc., and further identifying species characteristics of cordyceps.

The present invention has been provided as a result of such research, and in the present invention, a method of artificially cultivating cordyceps is selected by selecting a gourmet whose breeding technique has already been established and usefully used as a cultivation host for cordyceps. I have studied.

Gajam is an insect that has developed mass production technology in Korea and is used not only as an important insect for producing silk, a natural high-quality fiber, but also as a valuable medicinal ingredient for treating various diseases. Cordyceps is a bow that grows only in living insects. Since it is watery, insect breeding techniques have been established among a number of insects, and it has selected the gamm that human beings use usefully today. However, not all cordyceps can be cultivated on the basis of gajam, and research on cordyceps can be cultivated on the basis of gajam.

Thus, the present inventors made a thorough study in order to find the cordyceps fungi which can be the basis of the sleep, and as a result, confirmed the infection of pupa cordyceps (Cordyceps militaris) to gajam and completed the present invention.

Accordingly, an object of the present invention is to provide a method of artificial cultivation of Cordyceps militaris.

In order to achieve the above object, the artificial cultivation method of Cordyceps sinensis according to the present invention after spraying the spores of Cordyceps fungus Cordyceps militaris sp. Protecting and infecting and forming fruiting bodies from the infected larvae.

Spray inoculation of the cordyceps militaris strain spores in the artificial cultivation method of the present invention was diluted with water at a concentration of about 10 ⁸ spores / ㎖, and then under a temperature of 29 ~ 30 ℃, humidity of about 95% It is characterized by an operation for about 24 hours. In addition, the formation conditions of the fruiting body is characterized in that the temperature of 18 ~ 24 ℃, humidity of about 95%.

Other objects and functions of the present invention will become apparent to those skilled in the art from the following detailed description of the invention.

1 is a wild pupae cordyceps (Cordyceps militaris) formed mushrooms using the chrysalis of the insects of the stamens.

Figure 2 is a pupae cordyceps (Cordyceps militaris) artificially cultivated according to the present invention.

Hereinafter, the present invention will be described in detail.

Cordyceps sinensis is a medicinal mushroom that relies on transdermal infection caused by conidia scattering and attaching to the host insect epidermis that lives in the same place. Spores attached to the epidermis meet the conditions of temperature, humidity, etc. When the insects die and grow in the mycelial state in the body, when the insect dies, the mycelium is filled in the body, and the insects in the mycelium state are produced by the mechanism of germination of the fruiting body by meeting natural conditions such as proper temperature and humidity.

Cordyceps sinensis differs depending on species, germination conditions, timing and the like, and also various fruit forms, sizes, and colors. In the artificial cultivation of cordyceps of the present invention, the microorganisms to be applied are examined by examining whether the cordyceps fungus is infected with gamma. Was chosen.

TABLE 1

From Table 1, the actual cordyceps fungi can be seen that the host is different depending on the strain, in the present invention was selected as a cordyceps fungi (Cordyceps militaris) as a cordyceps that can be applied to artificial cultivation based on the basis of the gaze.

Chrysalis cordyceps in the present invention is obtained by culturing spores formed in wild chrysalis caterpillars (see Fig. 1) collected from Yasan in Korea. Specifically, the spores formed in wild pupa cordyceps were inoculated into PDA medium or brown rice medium, and then cultured for 30 to 40 days while maintaining the temperature of 20-25 ° C to form mycelia, thereby preparing a parent strain. By sprinkling spores on the epidermis of Gaza, fruiting bodies with scarlet-colored sack and head were obtained from infected pupa, and Cordyceps militaris, the Cordyceps fungus, was obtained on November 27, 1997. It was deposited with the Korea Microorganism Conservation Center (KFCC), affiliated with the Association, and was assigned accession number KFCC-11004.

The pupae Cordyceps sinensis strain (KFCC-11004) is a representative species belonging to the incomplete fungi of filamentous fungi, and has a wide range of host parasitic parasitic insects of various species of the species. Fruiting body of pupa cordyceps is about 5.0 ~ 10.0cm in size, and the scapula of head is semi-projection type.

In the artificial cultivation method of Cordyceps sinensis according to the present invention, the first step is to inoculate the chrysalis Cordyceps sinensis strain (KFCC-11004) to the epidermis to induce the infection of the bacteria. Inoculation was carried out by diluting the spores of the strain cultured in brown rice medium with water to a concentration of about 10 ⁸ spores / mL, and then using the nebulizer under the conditions of temperature of 29 ~ 30 ℃ and humidity of about 95% It is characterized by spraying evenly. Inoculation of bacteria is carried out for 24 hours.

After inoculating pupa puerto cordyceps strain KFCC-11004 into a gourmet, it is rapidly raised 2-4 times a day in accordance with the standard temperature and humidity, and the silkworms are protected, and the cocoons are protected at 15-20 ℃ after incubation. Select the hardened pupa. Cured pupa is infected with pupa Cordyceps sinensis strain, and when cut with a knife, it can be seen that orange Cordyceps sinensis mycelium is full.

In the second step of artificial cultivation, in order to induce fruiting body formation, the cured pupa is protected at a temperature of 18 to 24 ° C. and a humidity of about 95%. After 10-15 days, orange hyphae is formed on the pupal epidermis, and after several days, fruiting bodies start to form. After about 20-30 days, fruiting bodies grow to an average of about 5-10cm. This is the harvest season. The weight of the harvested Cordyceps sinensis is about 1.3 ~ 1.5g per one (chrysalis + fruiting body).

[Test Example 1]

In order to induce the temporary percutaneous infection of pupa Cordyceps sinensis strain (KFCC-11004), the inoculation method, inoculation concentration, inoculation time and inoculation temperature were tested under the conditions of 95% humidity under the conditions shown in Tables 2 and 3, and the results were examined. Table 2 and Table 3 are shown.

TABLE 2

TABLE 3

From Tables 2 and 3, the spores of the pupa Cordyceps sinensis strain (KFCC-11004) were diluted in water at a concentration of 2.0 × 10 ⁸ spores / ml for 24 hours in 5th stage larvae under the conditions of temperature 29-30 ° C. and 95% humidity. It can be seen that after spray inoculation, the cocoon exhibited a high infection rate of 50.0% when protected at 18 ° C.

[Test Example 2]

In order to determine the conditions that induce the formation of fruiting bodies from pupa infected with dead strains, the degree of fruiting was tested under the conditions described in Table 4, and the results are shown in Table 4.

TABLE 4

Degree of fruiting body formation by protection temperature

Table 4 shows that fruiting bodies are formed under a wide range of conditions at a temperature of 15 to 30 ° C. and a humidity of 95%. However, at 15 ° C. and 30 ° C., fruiting bodies are formed at a low rate and growth of fruiting bodies is extremely low. Therefore, it is preferable to form a fruiting body on the conditions of 18-24 degreeC of temperature, and 95% of humidity.

The longer the fruiting period is, the larger the fruiting body becomes, but it is desirable to harvest the offspring during the immature stage (30-40 days after protection) because the fruiting body loses its freshness because it loses weight and loses its freshness due to nutritional loss of host pupa. Do.

As described above, the artificial cultivation method of the present invention cultivating cordyceps by spraying the spores of the Cordyces militaris strain collected from wild cordyceps in the Gajam as the basis of the development of mass farming technology in Korea It is possible to mass-produce the Cordyceps sinensis by spray inoculation. Furthermore, more active research can be conducted in relation to the pharmacological effects of chrysalis of the chrysalis. In the pharmacological effect screening currently being conducted at the Natural Products Science Research Institute of Seoul National University, strong immune and anti-fatigue effects have been identified. In this case, it can be expected to improve national health and improve physical strength.

Claims (3)

In the method of artificial cultivation Cordyceps militaris, Spraying the spores of the Cordyceps militaris strain (KFCC-11004) onto the fifth larvae of Gajam, and then protecting the inoculated gom larva at 15-20 ° C. for infection; And A method of artificial cultivation of pupa caterpillar fungus comprising the step of forming an fruiting body from the infected larva. The spray inoculation of the spores according to claim 1, wherein the spores of the spores (KFCC-11004) are diluted with water to a concentration of about 10 ⁸ spores / ml, and then subjected to a temperature of 29 to 30 ° C. and a humidity of about 95%. Artificial cultivation method of pupa cordyceps, characterized in that performed for 24 hours. According to claim 1, wherein the conditions for forming fruiting bodies from the infected larva temperature 18 ~ 24 ℃, humidity is about 95% artificial cultivation method of pupa puertopsis.