KR102896317B1 - 니트레이트 환원효소의 돌연변이를 통한 식물 내 니트레이트 수준 조절 - Google Patents

니트레이트 환원효소의 돌연변이를 통한 식물 내 니트레이트 수준 조절

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Publication number
KR102896317B1
KR102896317B1 KR1020217023809A KR20217023809A KR102896317B1 KR 102896317 B1 KR102896317 B1 KR 102896317B1 KR 1020217023809 A KR1020217023809 A KR 1020217023809A KR 20217023809 A KR20217023809 A KR 20217023809A KR 102896317 B1 KR102896317 B1 KR 102896317B1
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South Korea
Prior art keywords
nicotiana tabacum
plant
tabacum plant
sequence
plant cell
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KR1020217023809A
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English (en)
Korean (ko)
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KR20210109578A (ko
Inventor
루치엔 보벳
프리스카 캄파노니
사이먼 괴페르트
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필립모리스 프로덕츠 에스.에이.
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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/10—Processes for modifying non-agronomic quality output traits, e.g. for industrial processing; Value added, non-agronomic traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/10—Chemical features of tobacco products or tobacco substitutes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/12—Steaming, curing, or flavouring tobacco
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0044—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on other nitrogen compounds as donors (1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y107/00—Oxidoreductases acting on other nitrogenous compounds as donors (1.7)
    • C12Y107/01—Oxidoreductases acting on other nitrogenous compounds as donors (1.7) with NAD+ or NADP+ as acceptor (1.7.1)
    • C12Y107/01001—Nitrate reductase (NADH) (1.7.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020217023809A 2018-12-30 2019-12-16 니트레이트 환원효소의 돌연변이를 통한 식물 내 니트레이트 수준 조절 Active KR102896317B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP18215913 2018-12-30
EP18215913.7 2018-12-30
PCT/EP2019/085321 WO2020141062A1 (en) 2018-12-30 2019-12-16 Modulation of nitrate levels in plants via mutation of nitrate reductase

Publications (2)

Publication Number Publication Date
KR20210109578A KR20210109578A (ko) 2021-09-06
KR102896317B1 true KR102896317B1 (ko) 2025-12-08

Family

ID=65030891

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020217023809A Active KR102896317B1 (ko) 2018-12-30 2019-12-16 니트레이트 환원효소의 돌연변이를 통한 식물 내 니트레이트 수준 조절

Country Status (8)

Country Link
US (1) US11976286B2 (https=)
EP (1) EP3902390A1 (https=)
JP (2) JP2022515435A (https=)
KR (1) KR102896317B1 (https=)
CN (1) CN113271768B (https=)
BR (1) BR112021012596A2 (https=)
MX (1) MX2021007628A (https=)
WO (1) WO2020141062A1 (https=)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7696368B2 (ja) * 2020-12-09 2025-06-20 日本たばこ産業株式会社 タバコ植物及びたばこ製品
CN112575005A (zh) * 2021-01-04 2021-03-30 昆明理工大学 一种提高烟草重金属镉胁迫抗性和降低镉富集的方法
WO2022199665A1 (zh) * 2021-03-25 2022-09-29 苏州齐禾生科生物科技有限公司 一种提高植物遗传转化和基因编辑效率的方法
CN120530195A (zh) 2023-02-02 2025-08-22 菲利普莫里斯生产公司 糖转运蛋白的调节
KR20250139370A (ko) 2023-02-02 2025-09-23 필립모리스 프로덕츠 에스.에이. 리신 케토글루타레이트 환원효소 코딩 유전자 조절
WO2025003105A1 (en) 2023-06-29 2025-01-02 Philip Morris Products S.A. Modulation of genes coding for glutamate dehydrogenase
WO2026057635A1 (en) 2024-09-12 2026-03-19 Philip Morris Products S.A. Modulation of nitrate levels in tobacco via mutation of nitrate reductase
WO2026082649A1 (en) 2024-10-15 2026-04-23 Philip Morris Products S.A. Modulation of alkaloid levels in tobacco via mutation of quinolinate synthase

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
BRPI0717355B1 (pt) 2006-10-13 2018-01-16 North Carolina State University Método para obtenção de uma planta transgênica de nicotina, método para obtenção de uma semente; construto de ácido nucléico recombinante; método de redução da conversão de nicotina em nornicotina em uma planta de nicotina
WO2009064771A2 (en) 2007-11-12 2009-05-22 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
EP2559766B1 (en) 2007-12-13 2017-10-18 Philip Morris Products S.A. Plants modified for reduced cadmium transport, derivative products, and related methods
PH12012501393B1 (en) 2010-01-15 2017-10-25 Univ North Carolina State Compositions and methods for minimizing nornicotine synthesis in tobacco
CA2794037A1 (en) 2010-03-22 2011-09-29 Philip Morris Products S.A. Modifying enzyme activity in plants
CN103228671B (zh) 2010-09-03 2017-08-15 菲利普莫里斯产品有限公司 植物中的重金属减少
EP2565271A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Threonine synthase from Nicotiana tabacum and methods and uses thereof
EP2565265A1 (en) 2011-09-02 2013-03-06 Philip Morris Products S.A. Isopropylmalate synthase from Nicotiana tabacum and methods and uses thereof
AU2012331235B2 (en) 2011-10-31 2017-06-15 Philip Morris Products S.A. Modulating beta-damascenone in plants
AP2015008563A0 (en) * 2012-12-21 2015-06-30 Philip Morris Products Sa Tobacco specific nitrosamine reduction in plants
EP3140404A1 (en) * 2014-05-08 2017-03-15 Philip Morris Products S.A. Reduction of nicotine to nornicotine conversion in plants
WO2015189693A1 (en) 2014-06-12 2015-12-17 King Abdullah University Of Science And Technology Targeted viral-mediated plant genome editing using crispr/cas9
WO2015197727A2 (en) * 2014-06-25 2015-12-30 Philip Morris Products S.A Modulation of nitrate content in plants
EP3198015A1 (en) 2014-09-26 2017-08-02 Philip Morris Products S.a.s. Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
RU2742725C2 (ru) 2015-09-09 2021-02-10 Филип Моррис Продактс С.А. Растения со сниженным содержанием аспарагина
EP3407704B1 (en) 2016-01-29 2024-08-14 Philip Morris Products S.A. Reducing cadmium accumulation in field grown tobacco plants
CN106690403A (zh) * 2017-03-09 2017-05-24 河南农业大学 用于降低白肋烟中硝酸盐积累的调节剂及其方法

Also Published As

Publication number Publication date
CN113271768B (zh) 2024-02-06
US20220186242A1 (en) 2022-06-16
EP3902390A1 (en) 2021-11-03
US11976286B2 (en) 2024-05-07
BR112021012596A2 (pt) 2021-11-30
WO2020141062A1 (en) 2020-07-09
JP2024180620A (ja) 2024-12-26
CN113271768A (zh) 2021-08-17
JP2022515435A (ja) 2022-02-18
KR20210109578A (ko) 2021-09-06
MX2021007628A (es) 2021-08-11

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