KR102854980B1 - 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주 - Google Patents

약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주

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KR102854980B1
KR102854980B1 KR1020187019759A KR20187019759A KR102854980B1 KR 102854980 B1 KR102854980 B1 KR 102854980B1 KR 1020187019759 A KR1020187019759 A KR 1020187019759A KR 20187019759 A KR20187019759 A KR 20187019759A KR 102854980 B1 KR102854980 B1 KR 102854980B1
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respiratory syncytial
syncytial virus
rsv
seq
protein
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KR20180085803A (ko
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피터 엘. 콜린스
우르술라 제이. 부흐홀츠
신디 루온고
Original Assignee
더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈
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Application filed by 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈 filed Critical 더 유나이티드 스테이츠 오브 어메리카, 애즈 리프리젠티드 바이 더 세크러테리, 디파트먼트 오브 헬쓰 앤드 휴먼 서비씨즈
Priority to KR1020257029082A priority Critical patent/KR20250134719A/ko
Publication of KR20180085803A publication Critical patent/KR20180085803A/ko
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KR1020187019759A 2015-12-11 2016-12-12 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주 Active KR102854980B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257029082A KR20250134719A (ko) 2015-12-11 2016-12-12 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562266199P 2015-12-11 2015-12-11
US62/266,199 2015-12-11
PCT/US2016/066146 WO2017100759A1 (en) 2015-12-11 2016-12-12 Recombinant respiratory syncytial virus strains with mutations in the m2-2 orf providing a range of attenuation phenotypes

Related Child Applications (1)

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KR1020257029082A Division KR20250134719A (ko) 2015-12-11 2016-12-12 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주

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KR20180085803A KR20180085803A (ko) 2018-07-27
KR102854980B1 true KR102854980B1 (ko) 2025-09-03

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KR1020257029082A Pending KR20250134719A (ko) 2015-12-11 2016-12-12 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주
KR1020187019759A Active KR102854980B1 (ko) 2015-12-11 2016-12-12 약독화 표현형의 범위를 제공하는 m2-2 orf 변이를 가지는 재조합 호흡기 세포융합 바이러스 균주

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US (2) US10655109B2 (enExample)
EP (1) EP3386539A1 (enExample)
JP (2) JP7079197B2 (enExample)
KR (2) KR20250134719A (enExample)
CN (1) CN109310752B (enExample)
AR (1) AR106956A1 (enExample)
AU (1) AU2016366771B2 (enExample)
BR (1) BR112018011674A2 (enExample)
CA (1) CA3007408A1 (enExample)
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US11273214B2 (en) * 2017-05-29 2022-03-15 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Recombinant chimeric bovine/human parainfluenza virus 3 expressing RSV G and its use
US20230279362A1 (en) * 2020-06-05 2023-09-07 The United States Of America, As Represented By The Secretary, Department Of Health And Human Live attenuated respiratory syncytial virus
TW202535439A (zh) 2023-11-17 2025-09-16 美商賽諾菲巴斯德公司 海藻糖疫苗調配物

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3224404A1 (de) 1982-06-30 1984-01-05 LuK Lamellen und Kupplungsbau GmbH, 7580 Bühl Reibungskupplung sowie verfahren zu deren montage
US7465574B2 (en) 1994-09-30 2008-12-16 Medimmune, Llc Recombinant RSV virus expression systems and vaccines
US6713066B1 (en) 1996-07-15 2004-03-30 The United States Of America As Represented By The Department Of Health And Human Services Production of attenuated respiratory syncytial virus vaccines involving modification of M2 ORF2
CA2230033C (en) 1995-09-27 2010-01-26 Peter L. Collins Production of infectious respiratory syncytial virus from cloned nucleotide sequences
US20040005542A1 (en) 2001-06-22 2004-01-08 Krempl Christine D Respiratory syncytial virus vaccines expressing protective antigens from promotor- proximal genes
AU3652202A (en) 2000-11-28 2002-06-11 Aviron Inc Recombinant rsv virus expression systems and vaccines
DE10118446A1 (de) 2001-04-12 2002-10-17 Thomas Hartung Prüfverfahren zur Untersuchung fließfähiger Medien auf Gehalte an mikrobiellen Toxinen
DK3511015T5 (da) 2012-04-13 2025-12-01 Us Health Genetisk stabil levende svækket respiratorisk syncytialvirusvaccine og fremstilling deraf
US20160228536A1 (en) * 2013-09-18 2016-08-11 Medimmune, Llc Recombinant respiratory syncytial virus (rsv) and vaccines
WO2017030997A1 (en) 2015-08-14 2017-02-23 Research Institute At Nationwide Children's Hospital Respiratory syncytial virus having altered ns1 protein function and related materials and methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JOURNAL OF VIROLOGY, 1997, pp. 8973-8982.*
SCIENCE TRANSLATIONAL MEDICINE, 2015.11.04., vol. 7, no. 312, pp. 1-9.*

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JP7079197B2 (ja) 2022-06-01
US11332721B2 (en) 2022-05-17
JP2018536428A (ja) 2018-12-13
CA3007408A1 (en) 2017-06-15
WO2017100759A1 (en) 2017-06-15
EP3386539A1 (en) 2018-10-17
BR112018011674A2 (pt) 2018-12-04
US10655109B2 (en) 2020-05-19
CN109310752B (zh) 2022-12-16
US20190040365A1 (en) 2019-02-07
KR20250134719A (ko) 2025-09-11
AU2016366771A1 (en) 2018-07-12
AU2016366771B2 (en) 2022-04-07
JP7357709B2 (ja) 2023-10-06
RU2018125246A3 (enExample) 2020-05-25
KR20180085803A (ko) 2018-07-27
JP2022088376A (ja) 2022-06-14
CN109310752A (zh) 2019-02-05
US20200283740A1 (en) 2020-09-10
AR106956A1 (es) 2018-03-07
RU2018125246A (ru) 2020-01-15

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