KR102661205B1 - Cosmetic compostion for improving skin immune system, and its preparation method - Google Patents

Abstract

The present invention provides a method for producing a cosmetic composition that has excellent efficacy in promoting skin immunity, and can provide a cosmetic composition that is excellent in promoting skin immunity by manufacturing by the above method.

Description

Cosmetic composition for improving skin immunity and method for manufacturing same {COSMETIC COMPOSTION FOR IMPROVING SKIN IMMUNE SYSTEM, AND ITS PREPARATION METHOD}

The present invention relates to a method for producing a cosmetic composition for improving skin immunity and a cosmetic composition prepared therefrom.

Immunity is a self-defense system that exists in the body and refers to the process by which the human body recognizes various substances or living organisms invading from the outside as foreign substances to itself, eliminates them, and metabolizes them. Although it protects itself from damage caused by external stimuli or invasion by pathogenic microorganisms, it can also cause damage to its own tissues, such as inflammatory reactions.

The skin, which has the largest cross-sectional area in the body, is the first defense tissue and the largest immune organ, protecting the body from various harmful environments through physical, chemical, and biological methods. Therefore, improving the skin's immunity begins with increasing the skin barrier function, which is the skin's primary defense function.

Among the skin's barrier functions, the chemical barrier function is maintained by the permeability barrier function of lipids between keratinocytes, low acidity of the skin surface, and antibacterial peptides in the stratum corneum. Antibacterial peptides are known to rapidly increase in expression level when infected with bacteria or viruses, thereby increasing antibacterial power. However, they are secreted even under normal conditions to strengthen immunity and act as an important component of innate immunity that defends the organism from external infectious agents.

β-defensin is one of the antibacterial peptides produced by the skin itself. It has an antibacterial effect by destroying the cell membrane of pathogens and is known as a representative skin immune substance. β-defensin has little effect on human cells, which are composed of positive ions. By developing a natural material that induces the expression of β-defensin in keratinocytes, it can be used as a cosmetic composition to improve skin immunity.

Fermentation technology is one of the technologies already widely used in the cosmetics manufacturing process. It is known that when fermentation technology is applied, new effects different from existing ones are shown or efficacy is increased, absorption into the skin increases, and harmful ingredients are converted into safe ingredients. To this day, many fermented materials are still used as cosmetic raw materials, but since most are manufactured through similar fermentation technologies, there is a need to develop new fermentation technologies.

Ultrasound is a mechanical sound wave with a frequency of 20 kHz or more and less than 10 MHz, and is used in various industrial fields. Especially in the food field, it is used to extract biologically active substances from plants, etc., and is also used to destroy microorganisms to extend the shelf life of food.

Even during the MERS outbreak in 2015, cosmetics that boost immunity received attention, and COVID-19 brought major changes to the domestic and international cosmetics market. As it became known that immunity was important for COVID-19, the skin's own There is a growing interest in improving human immunity. In this regard, keywords such as ‘probiotics’ and ‘skin microbiome’ continue to attract attention.

According to the '2020 Korea Skin Health Trend Report' (targeted to 3,326 Korean adult men and women) published by AHC Brand, when asked how thoughts on 'beauty care' have changed since the coronavirus, the answers were 'I want to increase my skin immunity' and 'I want to improve my skin's health.' The answer 'pursuing natural health' was the most common, and when asked about how 'microbiome cosmetics' will help the skin, the answer 'improvement of skin immunity' was confirmed to be at the top. It has been done.

Therefore, awareness of skin immunity is expected to continue to be of interest even after the coronavirus outbreak, and in addition, the efficacy of skin immunity is expected to be applicable to skin trouble problems during seasonal changes.

The present invention seeks to provide a method for producing a cosmetic composition that has excellent efficacy in promoting skin immunity.

In addition, the object is to provide a cosmetic composition manufactured by the above method that is excellent for improving skin immunity.

The method for producing a cosmetic composition for improving skin immunity according to the present invention includes the steps of (a) fermenting spinach powder and lactic acid bacteria; (b) sonication step: (c) additional fermentation step; and (d) recovering the extract.

The lactic acid bacteria are preferably Lactobacillus kunkeei.

The ultrasonic waves are preferably sound waves of 20 to 60 kHz.

The sonication step (b) preferably includes a plurality of sonication steps and an additional fermentation step.

It is more preferable that the sonication step (b) further includes (b-1) a short-term sonication step, (b-2) an additional fermentation step, and (b-3) a long-term sonication step.

It is preferable that the short-term ultrasonic treatment step (b-1) is performed as multiple ultrasonic treatments for a short period of time.

It is more preferable that the short-term sonication step (b-1) is performed 2 to 4 times for 5 to 15 minutes each.

It is preferable that the long-term sonication step (b-3) is sonicated for 2 to 4 hours.

As another embodiment of the present invention, it is desirable to provide a cosmetic composition for enhancing skin immunity prepared according to the production method of the present invention.

The composition is preferably in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder.

It is preferable that the composition is included in any one of a pack, band, or patch.

It is more preferable that the pack is any one of topical patch, sheet type, gauze type, cream type, and gel type.

The cosmetic composition includes hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair wax, and hair aerosol. , hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, hair moisturizer, It is preferable that it is a formulation selected from the group including hair mousse and hair spray.

The present invention provides a method for producing a cosmetic composition that has excellent efficacy in promoting skin immunity, and can provide a cosmetic composition that is excellent in promoting skin immunity by manufacturing by the above method.

Figure 1 schematically shows the manufacturing method according to the present invention.
Figure 2 schematically shows the effect of β-defensin expression according to Experimental Example 1.
Figures 3a to 3d schematically show the results of transepidermal water loss evaluation according to Experimental Example 2-1.
Figures 4A to 4D schematically show the results of measuring skin brightness and radiance according to Experimental Example 2-2.
Figures 5a and 5d schematically show the results of skin pigmentation measurement according to Experimental Example 2-2.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art. Accordingly, the embodiments of the present invention may be modified into various other forms, and the scope of the present invention is not limited to the embodiments described below.

Throughout the specification of the present invention, when a part “includes” a certain component, this means that it does not exclude other components but further includes other components, unless specifically stated to the contrary.

Throughout the specification of the present invention, when a step is said to be located “on” or “before” another step, this means not only when a step is in a direct time-series relationship with another step, but also when a step is in a direct time-series relationship with the other step, as well as a mixing step after each step. Likewise, the order of two steps can include the same rights as in the case of an indirect time-series relationship in which the time-series order can be changed.

The terms "about", "substantially", etc. used throughout the specification of the present invention are used to mean at or close to that value when manufacturing and material tolerances inherent in the stated meaning are presented, and the present invention Precise or absolute figures are used to aid understanding and to prevent unscrupulous infringers from taking unfair advantage of the disclosure. The term “step of” or “step of” used throughout the specification does not mean “step for.”

The method for producing a cosmetic composition for improving skin immunity according to the present invention includes the steps of (a) fermenting spinach powder and lactic acid bacteria; (b) sonication step: (c) additional fermentation step; and (d) recovering the extract.

The sonication step (b) preferably includes a plurality of sonication steps and an additional fermentation step.

It is more preferable that the sonication step (b) further includes (b-1) a short-term sonication step, (b-2) an additional fermentation step, and (b-3) a long-term sonication step.

It is preferable that the short-term ultrasonic treatment step (b-1) is performed as multiple ultrasonic treatments for a short period of time.

It is more preferable that the short-term sonication step (b-1) is performed 2 to 4 times for 5 to 15 minutes each.

It is preferable that the long-term sonication step (b-3) is sonicated for 2 to 4 hours.

When processing ultrasonic waves for extraction, frequencies in the low range (16 kHz to 100 kHz) are generally used, and if high frequencies are used, the components to be extracted may deteriorate due to excessive energy. Ultrasonic extraction uses ultrasonic waves to destroy the cell walls of plants so that the active ingredients can be easily extracted with a solvent. The mechanical effect of ultrasound allows the solvent to penetrate deeply into the extraction target, so high extraction yields can be obtained even without using organic solvents. there is. Because the extraction speed is also fast, it is used as an efficient extraction method.

Microbial sterilization using ultrasound can be largely explained by two principles: cavitation phenomenon and free radical. When energy is concentrated at one point due to cavitation, high temperature (5000 K) and high pressure (1000 atm) are instantly generated and bubbles are generated. In the process of collapsing bubbles, a fast and strong flow appears inside the liquid, and this shock wave can cause the cell membrane of microorganisms to collapse. Additionally, water molecules break down to generate free radicals, which act on cell membranes to destroy microorganisms. (J. Korean Soc. Manuf. Technol. Eng., 31:2, 2022, 101~107)

In the present invention, spinach was fermented using lactic acid bacteria, preferably Lactobacillus kunkeei, and ultrasonic treatment was performed at the same time to prepare an extract as shown in Figure 1.

The ultrasonic waves are preferably sound waves of 20 to 60 kHz. Specifically, by treating the 40 kHz frequency at a low intensity, spinach molecules were decomposed to increase the extraction efficiency of spinach. In addition, since the decomposed spinach is easier for lactic acid bacteria to use for fermentation, the fermentation efficiency was also attempted to be increased. When the 40 kHz frequency is treated at a low intensity for a short period of time, the sterilization effect on microorganisms is only about 15 to 20%, so the microorganisms are not completely killed and fermentation can be continued. Afterwards, when treated with a high frequency of 40 kHz, the cell membrane and cell wall of the lactic acid bacteria are destroyed, causing the lactic acid bacteria to die. At this time, as the lactic acid bacteria die, enzymes and active ingredients within the lactic acid bacteria are released, which was intended to be used for fermentation.

As another embodiment of the present invention, it is desirable to provide a cosmetic composition for enhancing skin immunity prepared according to the production method of the present invention.

The composition according to the present invention can be in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder, but is not limited thereto.

For example, lotions are similar to solutions, but tend to be thicker and more emollient in nature. Lotions are usually a mixture of water and oil and often contain less alcohol than a solution.

For example, creams are an emulsion of approximately equal proportions of oil and water. It penetrates well into the outer layer of the skin's stratum corneum. Creams are thicker than lotions and retain their shape when removed from the container.

Additionally, the composition of the present invention may be included in any one of a pack, band, or patch, but is not limited thereto.

The pack may be in the form of any one of topical patch, sheet type, gauze type, cream type, and gel type.

The pack may be any one selected from a mask pack, hand pack, foot pack, calf pack, hair pack, thigh pack, abdominal pack, side pack, forearm pack, chin pack, and neck pack.

The selection of suitable ingredients that can be added to the present invention, as will be apparent to those skilled in the art, depends to some extent on whether the external skin composition of the present invention is formulated as a solution, lotion, cream, ointment, gel, foam, or transdermal patch. something to do.

For example, it may include colorants (pigments), flavoring agents (flavors), suspending agents, emulsifiers, solubilizers, stabilizers, isotonic agents, pH adjusters, viscosity adjusters, solvents, etc. The ingredients and contents commonly added to cosmetic compositions will be apparent to those skilled in the art.

On the other hand, the cosmetic composition of the present invention can be used as hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, hair massage cream, hair Wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair lacquer, It may be a formulation selected from the group including hair moisturizer, hair mousse, and hair spray, but is not limited thereto.

Hereinafter, the present invention will be described in more detail through specific examples and experimental examples of the composition according to the present invention.

(Example 1) Spinach fermentation-preparation of ultrasonic extract

As shown in Figure 1, Lactobacillus kunkeei strain was inoculated into MRS broth medium and cultured with agitation at 37°C for 24 hours. The bacterial culture was diluted with MRS broth medium to prepare a bacterial inoculum so that OD _600nm = 1.00.

Spinach to be used for fermentation was dried, finely ground using a grinder, and powdered. Spinach powder was sterilized at 121°C for 15 minutes and then sufficiently cooled to room temperature. MRS broth medium was dispensed into the sterilized spinach powder and moistened for approximately 30 minutes to 1 hour to ensure sufficient moisture.

Bacterial inoculum with OD _600nm = 1.00 was evenly inoculated into the moistened spinach powder and mixed evenly using a sterilized spatular. Fermentation was carried out at 37°C, and the fermented product was recovered 2 days after fermentation.

After adding an appropriate amount of MRS broth medium to the fermented L. kunkeei spinach, the mixture was treated three times for 10 minutes at a low frequency of 40 kHz using a sonicator (powersonic 420, hwashin). Afterwards, fermentation was carried out at 37°C, and the fermented product was recovered 1 day after fermentation.

The fermented L.kuekeei spinach was treated with a sonicator (powersonic 420, hwashin) at a high frequency of 40 kHz for 3 hours to extract spinach and induce the death of lactic acid bacteria. Afterwards, additional fermentation was performed at 37°C, and the fermented product was recovered 1 day after fermentation.

For the final fermented product, the lactic acid bacteria cells and spinach powder were filtered through a 0.2㎛ filter to obtain the final spinach fermentation-ultrasonic extract.

(Comparative Example 1) Preparation of spinach ethanol extract

The dried spinach was finely ground using a grinder and powdered. After mixing spinach powder and 70% ethanol, an extraction solution was prepared for 3 hours at room temperature using a stirrer. After primary filtration of the extraction solution using non-woven filter paper, the primary filtrate was filtered using no. It was filtered under reduced pressure using 5C filter paper. This was concentrated under reduced pressure to completely remove the ethanol component in the extract, thereby obtaining a spinach ethanol extract.

(Experimental Example 1) Confirmation of β-defensin expression

Human Epidermal Keratinocyte (neonatal) cells were cultured on a plate for 24 hours, then treated with Example 1 and Comparative Example 1 and cultured for 24 hours. Cells were recovered, RNA was isolated, and RT-PCR was performed to confirm gene expression level.

As shown in Figure 2, the expression of β-defensin 2 and 3 was increased by the fermented spinach-ultrasonic extract of Example 1, and it was confirmed that the efficacy was superior to the spinach ethanol extract of Comparative Example 1. Through this, it can be confirmed that spinach fermentation-ultrasonic extract provides excellent effects in improving skin immune function.

(Example 2) Preparation of cosmetic raw material composition for external skin application

A cosmetic raw material composition for external use on the skin was prepared according to the composition in Table 1 below.

Ingredients content (%) 1,3-Butylene glycol 50.0 Water 49.5 Example 1 0.5

(Example 3) Manufacturing of external skin cosmetics in toner formulation

As a cosmetic composition for external use on the skin, a toner formulation was prepared according to the composition in Table 2 below.

Ingredients content (%) Water 81.63 Disodium EDTA 0.05 Betaine 0.50 Glycerin 3.00 1.2-Hexandiol 2.00 1,3-Butylene glycol 10.00 PEG-60 Hydrogenated castor oil 0.60 Example 2 2.00

(Experimental Example 2) Skin effectiveness evaluation

The effect of the toner formulation containing the fermented spinach-ultrasonic extract prepared in Example 1 on reducing transepidermal water loss and improving skin brightness was evaluated through clinical tests. As a sample, a toner containing 2% of spinach fermentation-ultrasonic extract cosmetic raw material was used, and the degree of improvement was measured before and after use.

Twenty adult male and female test subjects between the ages of 25 and 60 were asked to use the toner sample twice a day (morning and evening) for two weeks on their entire face (forehead, both cheeks) and left arm forearm. Transepidermal water loss, skin brightness and radiance, and improvement in pigmentation were confirmed before, 1 week after, and 2 weeks after use.

(Experimental Example 2-1) Evaluation of transepidermal water loss

In order to evaluate the effect on skin barrier function (transepidermal water loss, TEWL) after 2 weeks of use of the toner of Example 3, transepidermal water loss was measured using a Vapometer® after 1 to 2 weeks of use of the toner sample. was measured and the results are shown in Figures 3A to 3D.

As shown in Figures 3a to 3d, the amount of transepidermal water loss decreased two weeks after using the formulation containing the spinach fermented-ultrasonic extract, which confirms that the spinach-fermented ultrasonic extract provides an excellent effect in improving skin barrier function. there is.

(Experimental Example 2-2) Evaluation of skin condition improvement effect

After 2 weeks of using the toner of Example 3, in order to measure the clinical skin brightening effect, after 1 to 2 weeks of using the toner sample, skin brightness and radiance (Luminance, ΔL*) were measured using skin color catch®. ) and skin pigmentation (Individual Typological Angle, ITA°) were measured, and the results are shown in FIGS. 4A to 4D and 5A to 5D.

<Measurement of skin brightness and radiance>

Skin brightness and radiance were measured using skin color catch® on the same area before and after using the toner sample. Measurements were performed three times, and the average value was used as evaluation data and is shown in Figures 4A to 4D.

As shown in Figures 4a to 4d, the Luminance value increased two weeks after using the formulation containing the spinach fermentation-ultrasonic extract, which confirms that the spinach fermentation-ultrasonic extract provides an excellent effect in improving skin brightness and radiance. there is.

<Skin pigment measurement>

Skin pigmentation was measured on the same area before and after using the toner sample using skin color catch®, and measurements were performed three times, and the average value was used as evaluation data and is shown in Figures 5A to 5D.

As shown in Figures 5A to 5D, the ITA° value increased 2 weeks after using the formulation containing the spinach fermentation-ultrasonic extract, which confirms that the spinach fermentation-ultrasonic extract provides an excellent effect in improving skin pigmentation. .

Although the embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and variations are possible without departing from the technical spirit of the present invention as set forth in the claims. This will be self-evident to those with ordinary knowledge in the technical field.

Claims (13)

(a) fermenting spinach powder and lactic acid bacteria;
(b-1) Short-term sonication step of 2-4 sonications for 5-15 minutes each:
(b-2) additional fermentation step;
(b-3) long-term sonication step;
(c) additional fermentation steps; and
(d) recovering the extract; a method for producing a cosmetic composition for skin moisturizing and skin whitening, comprising: The method for producing a cosmetic composition for skin moisturizing and skin whitening according to claim 1, wherein the lactic acid bacteria are Lactobacillus kunkeei. The method for producing a cosmetic composition for skin moisturizing and skin whitening according to claim 1, wherein the ultrasonic waves are sound waves of 20 to 60 kHz. The method for producing a cosmetic composition for skin moisturizing and skin whitening according to claim 1, wherein the long-term ultrasonic treatment step (b-3) is ultrasonic treatment for 2 to 4 hours. delete delete delete A cosmetic composition for skin moisturizing and skin whitening comprising the extract obtained by the method according to claim 1. The cosmetic composition for skin moisturizing and skin whitening according to claim 8, which further has a skin immunity enhancing effect. The cosmetic composition for skin moisturizing and skin whitening according to claim 9, wherein the composition is in the form of any one of aerosol, liquid, solution, gel, paste, cream, ointment, foam, and powder. The cosmetic composition for skin moisturizing and skin whitening according to claim 9, wherein the composition is included in any one of a pack, a band, or a patch. The cosmetic composition for skin moisturizing and skin whitening according to claim 11, wherein the pack is one of a topical patch, sheet type, gauze type, cream type, and gel type. The method of claim 9, wherein the cosmetic composition is used in hair tonic, hair conditioner, hair essence, hair lotion, hair nutrition lotion, hair shampoo, hair rinse, hair treatment, hair cream, hair nutrition cream, hair moisture cream, and hair massage cream. , hair wax, hair aerosol, hair pack, hair nutrition pack, hair soap, hair cleansing foam, hair oil, hair dryer, hair preservation treatment, hair dye, hair waving agent, hair bleach, hair gel, hair glaze, hair dresser, hair A cosmetic composition for skin moisturizing and skin whitening, characterized in that it is a formulation selected from the group including lacquer, hair moisturizer, hair mousse, and hair spray.