KR102654317B1 - Composition for skin moisturizing and skin barrier strengthening containing avena strigosa seed extract as an active ingredient - Google Patents

Abstract

This relates to a composition for skin moisturizing and skin barrier strengthening containing black oat (Avena Strigosa Seed) extract as an active ingredient. More specifically, the black oat extract is characterized by excellent skin moisturizing and skin barrier strengthening effects.

Description

Composition for skin moisturizing and skin barrier strengthening containing black oat extract as an active ingredient {COMPOSITION FOR SKIN MOISTURIZING AND SKIN BARRIER STRENGTHENING CONTAINING AVENA STRIGOSA SEED EXTRACT AS AN ACTIVE INGREDIENT}

The present invention relates to a composition for skin moisturizing and skin barrier strengthening containing black oat (Avena Strigosa Seed) extract as an active ingredient. More specifically, the black oat extract is characterized by excellent skin moisturizing and skin barrier strengthening effects. There is.

The moisture balance of the skin is controlled by the epidermis. The epidermis is made up of dead cells, but it plays a very important role in maintaining moisture in the skin. The human body is mostly composed of water, which plays an important role in maintaining life. In normal skin, 70% of the moisture is contained within the body and 20% is contained in the stratum corneum, which supports the skin's elasticity and flexibility. It is known that when the moisture content of the stratum corneum is less than 10%, the skin becomes dry and causes skin problems. The moisture content in the stratum corneum created by regular keratinization is always maintained at 10-20%. This is due to the sebum film, which is a mixture of sweat and sebum, protecting the skin from the outside and preventing moisture from evaporating from the stratum corneum. As such, the skin is the largest organ in the human body and plays various roles such as barrier function, temperature control function, excretion function, and respiratory function to protect other important organs of the human body from the outside and prevent finite components from escaping. It is a very important institution.

However, artificial temperature control of cooling/heating due to recent environmental changes or changes in lifestyle patterns, skin stress due to various stresses and environmental pollution occurring in social life, frequent face washing due to makeup habits, and natural skin damage due to aging. Due to various causes such as aging, the moisture in the stratum corneum decreases, causing the skin to dry out, the surface to become rough, the skin to become dull, lose moisture, and appear lifeless. Therefore, it is necessary to use compositions for strengthening the skin barrier or moisturizing the skin. The need is increasing.

Meanwhile, oats are known to be rich in soluble dietary fiber, protein, vitamins and minerals. Avenanthramide, phenolic acid, and flavonoid are known to be the most important phenolic compounds in oat ingredients, and phenolic compounds have been reported to have antioxidant, anti-inflammatory, anti-allergic, and anti-carcinogenic biological activities. . The antioxidant and anti-inflammatory activities of oats and their indicator components, avenanthramides A, B, and C, have been previously reported, but they are also used as skin protectants through tyrosinase inhibition, such as whitening, inhibition of pigmentation, and inhibition of freckle pigment formation. The role is unknown.

Accordingly, while researching to solve the above problems, the present inventors developed a composition containing black oat (Avena Strigosa Seed) extract as an active ingredient, and found that the composition had excellent skin moisturizing and skin barrier strengthening effects. The discovery led to the completion of the present invention.

In this regard, Republic of Korea Patent Publication No. 10-2023-0071426 discloses a complex functional cosmetic composition for antioxidant, skin whitening and wrinkle improvement containing oat radicle extract or a fraction thereof as an active ingredient.

The present invention was made to solve the problems of the prior art, and its purpose is to provide a composition for moisturizing the skin and strengthening the skin barrier containing black oat (Avena Strigosa Seed) extract as an active ingredient.

Additionally, the object is to provide a cosmetic composition containing the composition for moisturizing the skin and strengthening the skin barrier.

As a technical means for achieving the above-described technical problem, one aspect of the present invention is,

A composition for moisturizing skin and strengthening the skin barrier containing black oat (Avena Strigosa Seed) extract as an active ingredient is provided.

The black oat (Avena Strigosa Seed) extract is a group consisting of water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol, and combinations thereof. It may be characterized as being extracted with a solvent selected from.

The black oat (Avena Strigosa Seed) extract may be extracted by extracting and depositing black oats in a solvent, filtering the deposit, and concentrating the filtrate under reduced pressure.

In addition, another aspect of the present invention is,

A cosmetic composition comprising the composition for moisturizing the skin and strengthening the skin barrier is provided.

The cosmetic composition includes lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, It may be characterized by having a formulation selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser formulations.

The composition according to the present invention as described above may have excellent skin moisturizing and skin barrier strengthening effects by containing black oat (Avena Strigosa Seed) extract as an active ingredient.

Figure 1 is a graph showing the cytotoxicity test results of black oat extract and regular oat extract prepared according to an example and comparative example of the present invention.
Figures 2 and 3 are graphs showing moisturizing test results of black oat extract prepared according to an embodiment of the present invention.
Figures 4 and 5 are graphs showing the results of barrier tests of black oat extract prepared according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail. However, the present invention can be implemented in various different forms, and the present invention is not limited to the embodiments described herein, and the present invention is only defined by the claims to be described later.

Additionally, the terms used in the present invention are only used to describe specific embodiments and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In the entire specification of the present invention, 'including' a certain element means that other elements may be further included rather than excluding other elements, unless specifically stated to the contrary.

The first aspect of the present application is,

A composition for moisturizing skin and strengthening the skin barrier containing black oat (Avena Strigosa Seed) extract as an active ingredient is provided.

Hereinafter, the composition for moisturizing skin and strengthening the skin barrier according to the first aspect of the present invention will be described in detail.

In one embodiment of the present application, the composition for moisturizing skin and strengthening the skin barrier may contain black oat (Avena Strigosa Seed) extract as an active ingredient.

In one embodiment of the present application, the black oat (Avena Strigosa Seed) extract may have an excellent extraction yield and may not have cytotoxicity even at high concentrations. Additionally, it may have excellent skin moisturizing and skin barrier strengthening effects. Therefore, the composition according to the present invention has skin moisturizing and skin barrier strengthening effects, and has excellent extraction yield and is not cytotoxic even at high concentrations, so it is very economical to manufacture and has excellent skin stability.

In one embodiment of the present application, the skin moisturizing effect of the black oat (Avena Strigosa Seed) extract was demonstrated by the expression effect of moisturizing-related genes (AQP3, HAS2), as described in the experimental example, and through this, the black oat (Avena Strigosa Seed) extract (Avena Strigosa Seed) extract increases the moisture content of the skin, so the composition containing it may have a moisturizing effect.

In one embodiment of the present application, strengthening the skin barrier may mean strengthening the barrier function of the stratum corneum located in the outermost layer of the skin. One of the most important functions performed by the skin (epidermis) is the physical barrier function that defends against various external factors (physical and chemical irritants, pollutants, chemicals, ultraviolet rays, etc.). As the skin barrier rapidly transitions from the granular layer to the stratum corneum, keratin intermediate fine fibers become entangled to form a fiber bundle, and cross-link with protein components in the keratin glass granules and other cytoplasmic proteins to form a keratin protein membrane surrounding the inside of the cell membrane. Cell membranes are replaced. The keratin protein membrane and the keratin lipid membrane combine to form a cornified envelope that forms the border of keratinocytes, completing a complete skin barrier structure. FLG, IVL, or LOR strengthens the skin barrier by crosslinking various proteins necessary for the formation of keratin hypertrophic membrane and contributing to the formation of keratin lipid membrane by linking long chain hydroxyceramide with the protein. .

Additionally, the skin acts as a primary barrier that protects the organism from the external environment. Keratinocytes, which mainly make up the epidermis of the skin, play the role of creating a skin barrier through division and differentiation. Skin diseases occur when the skin barrier is not created normally or when keratinocytes differentiate abnormally. In this case, strengthening the skin barrier can be induced by allowing keratinocytes to differentiate normally and form a skin barrier. Differentiation of keratinocytes results in cell cycle arrest or changes in cell shape as a result of changes in the expression of regulated genes. At this time, genes whose expression increases in relation to differentiation include FLG, IVL, or LOR. When keratinocytes differentiate, the structural proteins inside the cell membrane combine with FLG, IVL, or LOR to form a hard structure called the cornified cell envelope, thereby strengthening the skin barrier.

Therefore, in this respect, the black oat (Avena Strigosa Seed) extract according to the present invention may strengthen the skin barrier function by forming a skin barrier by increasing the expression of FLG, IVL, or LOR, factors related to keratinocyte differentiation. there is.

In one embodiment of the present application, the black oat (Avena Strigosa Seed) extract is water, anhydrous or hydrous ethanol, methanol, propanol, butanol, acetone, ethyl acetate, hexane, benzene, chloroform, glycerin, butylene glycol, propylene glycol. and combinations thereof, preferably extracted with a solvent of water or ethanol, more preferably extracted with high temperature water (purified water). It may be possible. That is, the black oat (Avena Strigosa Seed) extract may most preferably be extracted using hot water extraction. At this time, the hot water extraction may be performed at a temperature of 80°C to 100°C for 30 minutes to 2 hours, and according to one embodiment of the present invention, it may be performed at a temperature of about 95°C for 1 hour.

In one embodiment of the present application, the black oat (Avena Strigosa Seed) extract may be characterized in that black oats are extracted and deposited in a solvent, and then the sediment is filtered and the filtrate obtained is concentrated under reduced pressure. .

In one embodiment of the present application, the filtration may be performed using a membrane filter, but is not limited thereto. When the membrane filter is used, the pore size of the filter is about 0.1 μm to 1 μm. It may be. Additionally, the reduced pressure concentration may be performed using a reduced pressure concentrator, but is not limited thereto, and any conventional reduced pressure concentration method may be used.

In one embodiment of the present application, the extract concentrated under reduced pressure as described above may be freeze-dried to remove all solvents, and through this, a black oat (Avena Strigosa Seed) extract in powder form may be obtained.

The second aspect of the present application is,

A cosmetic composition comprising a composition for moisturizing skin and strengthening the skin barrier according to the first aspect of the present application is provided.

Detailed description of parts overlapping with the first aspect of the present application has been omitted, but the content described in the first aspect of the present application can be applied equally even if the description is omitted in the second aspect.

Hereinafter, the cosmetic composition according to the second aspect of the present application will be described in detail.

In one embodiment of the present application, the content of the composition for moisturizing the skin and strengthening the skin barrier may be 0.0001 parts by weight to 10 parts by weight based on 100 parts by weight of the cosmetic composition. At this time, if the content of the composition for moisturizing the skin and strengthening the skin barrier is less than 0.0001 parts by weight, a problem may occur in which the efficacy of the black oat (Avena Strigosa Seed) extract is reduced, and if it is more than 10 parts by weight, the cosmetic composition Problems may arise where the formulation stability is reduced.

In one embodiment of the present application, the cosmetic composition includes lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, and foundation. It may be characterized by having a formulation selected from the group consisting of formulations of essence, nutritional essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser formulation.

More specifically, the cosmetic composition may be formulated containing a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, for example, solutions, gels, solids, pasty anhydrous products, emulsions obtained by dispersing the oil phase in the water phase, suspensions, microemulsions, microcapsules, microgranules or ionic forms (liposomes) and non-ionic forms. It may be provided in the form of an ionic vesicular dispersion, or in the form of a cream, skin, lotion, powder, ointment, spray or conceal stick. It can also be used in the form of foam or in the form of an aerosol composition further containing compressed propellant. These compositions can be prepared according to conventional methods in the art. In addition, the composition according to the present invention contains fatty substances, organic solvents, solubilizers, thickeners, gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or non-ionic. such as emulsifiers, fillers, sequestering agents, chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or any other ingredients commonly used in cosmetics. May contain auxiliaries commonly used in the fields of cosmetology or dermatology. The auxiliary agent may be introduced in an amount commonly used in the fields of cosmetology or dermatology. Additionally, the composition of the present invention may contain a substance that promotes skin absorption to increase the skin improvement effect.

Additionally, in another embodiment of the present application, an external skin preparation composition may be provided as a replacement for the cosmetic composition. At this time, the content of the composition for moisturizing the skin and strengthening the skin barrier may be 0.0001 parts by weight to 10 parts by weight based on 100 parts by weight of the composition for external skin application.

In another embodiment of the present application, the composition for external application for skin may be used as an external composition for skin application for anti-aging, which may improve skin elasticity and have excellent anti-wrinkle and anti-wrinkle effects. Meanwhile, the composition of the present invention can be used as a moisturizing external skin composition, which can strengthen the skin barrier function and induce differentiation of skin keratinocytes. Therefore, it can be useful as an external skin composition to prevent or improve dry skin, atopic dermatitis, contact dermatitis, or psoriasis caused by incomplete epidermal differentiation.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

Example 1. Preparation of black oat extract (hot water extraction)

Black oats were added to purified water, extracted at 95°C for 1 hour, and cooled at room temperature for 2 hours.

Afterwards, the extract was filtered through a 0.45 μm membrane filter, and the filtered extract was concentrated using a reduced pressure concentrator.

Finally, black oat extract powder was prepared by lyophilizing the concentrated extract to remove all purified water.

Example 2. Preparation of black oat extract (ethanol extraction)

Black oat extract powder was prepared in the same manner as in Example 1, except that black oats were immersed in 70% ethanol rather than purified water, and extraction was repeated three times over three days.

Comparative Example 1. Preparation of common oat extract (hot water extraction)

A common oat extract was prepared using the same method as in Example 1, except that regular oats were used instead of black oats.

Comparative Example 2. Preparation of common oat extract (ethanol extraction)

A common oat extract was prepared using the same method as in Example 2, except that regular oats were used instead of black oats.

Experimental Example 1. Yield measurement of extract

The yields of the extracts prepared according to the above examples and comparative examples were measured and shown in Table 1 below.

[Table 1]

As shown in Table 1, it was confirmed that in the case of the black oat extract according to the example of the present invention, the extract was obtained in a higher yield than the general oat extract.

Experimental Example 2. Cytotoxicity test evaluation

HaCaT MTT assay was performed to evaluate the cytotoxicity of the extracts prepared according to Example 1 and Comparative Example 1, and the results are shown in Table 2 and Figure 1, respectively.

[Table 2]

As shown in Table 2 and Figure 1, the black oat extract prepared according to Example 1 of the present invention did not show toxicity to HaCaT cells at concentrations of up to 500 μg/mL or less. On the other hand, the extracts according to Comparative Examples 1 and 2 did not show toxicity at concentrations of 50 μg/mL and 25 μg/mL or less, respectively, and the black oat extract prepared according to Example 1 was compared to the extracts according to Comparative Examples 1 and 2. It was confirmed that it did not have cytotoxicity even at significantly higher concentrations.

Experimental Example 3. Moisturizing effect evaluation

In order to evaluate the moisturizing efficacy of the extract prepared according to the above example, the expression effect of the moisturizing-related gene (AQP3) was measured through real-time polymerase chain reaction (qRT-PCT), and the results are shown in Table 3 and Figure 2 below. are shown respectively.

[Table 3]

As shown in Table 3 and Figure 2, the black oat extract prepared according to an example of the present invention has an excellent expression effect of a moisturizing-related gene (AQP3) even at a low concentration compared to panthenol, which is known to have a skin moisturizing effect. As a result, it was confirmed that it had a moisturizing effect.

In addition, the expression effect of moisturizing-related gene (HAS2) was measured through real-time polymerase chain reaction (qRT-PCT), and the results are shown in Table 4 and Figure 3, respectively.

[Table 4]

As shown in Table 4 and Figure 3, the black oat extract prepared according to an example of the present invention expresses a similar or higher level of a moisturizing-related gene (HAS2) at a lower concentration than panthenol, which is known to have a skin moisturizing effect. It was confirmed that it has excellent moisturizing effect by showing the effect.

Experimental Example 3. Barrier efficacy evaluation

In order to evaluate the barrier efficacy of the extract prepared according to the above example, the expression effect of barrier-related genes (FLG, IVL, and LOR) was measured through real-time polymerase chain reaction (qRT-PCT), and the results are shown in the table below. 5 and 6 and Figures 4 and 5, respectively (Table 5 and Figure 4 show the effect of FLG expression / Table 6 and Figure 5 show the effect of LOR expression).

[Table 5] - FLG expression effect

[Table 6] - LOR expression effect

As shown in Tables 5 and 6 and Figures 4 and 5, the black oat extract prepared according to the examples of the present invention has excellent barrier efficacy by showing the expression effect of barrier-related genes (FLG and LOR). I was able to confirm.

Above, the present invention has been described in detail along with preferred embodiments with reference to the drawings, but the scope of the technical idea of the present invention is not limited to these drawings and examples. Accordingly, various modifications or equivalent embodiments may exist within the scope of the technical idea of the present invention. Therefore, the scope of rights of the technical idea according to the present invention should be interpreted in accordance with the claims, and technical ideas within the scope equivalent or equivalent thereto should be interpreted as falling within the scope of the rights of the present invention.

Claims (5)

Contains black oat (Avena Strigosa Seed) extract as an active ingredient,
The black oat (Avena Strigosa Seed) extract is a composition for moisturizing skin and strengthening the skin barrier, which is obtained by extracting black oats by hot water extraction and precipitation, then filtering the sediment and concentrating the filtrate under reduced pressure.
delete delete A cosmetic composition comprising the composition for moisturizing skin and strengthening the skin barrier according to claim 1.
According to clause 4,
The cosmetic composition includes lotion (skin lotion), skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage cream, nutritional cream, moisture cream, hand cream, foundation, essence, nutritional essence, pack, A cosmetic composition characterized by having a formulation selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser formulation.