KR102635197B1 - Oral composition for effective prevention of biofilm formation - Google Patents

Abstract

The present invention relates to baking soda; L-menthol; At least one selected from sodium saccharin and beta-cyclodextrin; And at least one selected from sodium carboxymethyl cellulose, sodium alginate, and xanthan gum; containing the baking soda in an amount of 10 to 70% by weight based on the total weight of the composition. Disclosed is a composition for oral use. Specifically, the oral composition according to the present invention contains a high content of baking soda, showing an excellent biofilm formation inhibition effect, while improving the unique unpleasant taste and improving formulation stability, thereby providing an excellent feeling of use.

Description

Oral composition effective in inhibiting biofilm formation {ORAL COMPOSITION FOR EFFECTIVE PREVENTION OF BIOFILM FORMATION}

The present invention relates to an oral composition that is effective in suppressing biofilm formation. More specifically, it contains a high content of baking soda, which is effective in suppressing biofilm formation, while improving the unique taste of baking soda and improving the formulation separation phenomenon and feeling of use. It relates to a composition for oral use.

In general, oral diseases are caused by various bacteria living in the oral cavity, and when harmful oral bacteria gather together, they form a biofilm, which is a bacterial film on the teeth, causing cavities and gum disease. To inhibit and remove biofilm formation, abrasives are used physically and antibacterial agents are used chemically. Abrasives used in oral products include high molecular materials such as calcium carbonate, silica, aluminum hydroxide, calcium phosphate, baking soda, polyethylene, and polyvinyl chloride. In addition, antibacterial agents used in oral products include cetylpyridinium chloride, hexalkonium chloride, benzalkonium chloride, decarinium chloride, chlorhexidine, triclosan, thymol, isopropylmethylphenol, and alkyldiaminoethylglycane chloride. There are raw material ingredients, etc.

Among these, baking soda, which is particularly effective in suppressing biofilm formation, has the advantage of effectively suppressing biofilm formation and providing a refreshing feeling when used, but there was a problem of low preference due to the unique pungent taste of baking soda. In addition, there was a phenomenon of separation of the formulation, which had the disadvantage of flowing out in a liquid state or being inconvenient due to the flowing texture when used.

Republic of Korea Patent Publication No. 10-2016-0112919

The purpose of the present invention is to provide an oral composition that is effective in suppressing biofilm formation with improved feeling of use by improving taste preference and formulation stability while containing a high content of baking soda.

In order to achieve the above object, one embodiment of the present invention is baking soda; L-menthol; At least one selected from sodium saccharin and beta-cyclodextrin; And one or more selected from sodium carboxymethyl cellulose, sodium alginate, and xanthan gum;

Provided is an oral composition containing the baking soda in an amount of 10 to 70% by weight based on the total weight of the composition.

The oral composition according to the present invention contains a high content of baking soda, showing an excellent biofilm formation inhibition effect, while improving the unique unpleasant taste and improving formulation stability, thereby providing an excellent feeling of use.

Unless otherwise defined, all technical and scientific terms used in this specification have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein is well known and commonly used in the art.

“Biofilm” is a type of thin bacterial film created when microorganisms in the oral cavity adhere to the surface of teeth, and is also called dental bacterial film or plaque. This biofilm causes plaque and tartar to form, which can eventually lead to gum disease.

Hereinafter, the present invention will be described in detail.

In one aspect, the present invention includes baking soda; L-menthol; At least one selected from sodium saccharin and beta-cyclodextrin; And at least one selected from sodium carboxymethyl cellulose, sodium alginate, and xanthan gum; containing the baking soda in an amount of 10 to 70% by weight based on the total weight of the composition. It may be related to an oral composition. Specifically, the oral composition of the present invention may be used to inhibit biofilm formation.

According to one embodiment of the present invention, the baking soda is used in an amount of 10% by weight or more, 15% by weight, 20% by weight, 25% by weight, 30% by weight, 32% by weight or more, and 35% by weight based on the total weight of the composition. or more, 37% by weight or more, 40% by weight or more, 42% by weight or more, 45% by weight or more, 47% by weight or more, 50% by weight or more, 52% by weight or more, 55% by weight or more, 57% by weight or more, 60% by weight. It may be contained in an amount of 62% by weight or more, 65% by weight or more, or 67% by weight or more. Additionally, the baking soda may be contained in an amount of 70% by weight or less, 67% by weight, 65% by weight or less, and 62% by weight relative to the total weight of the composition. % or less, 60% or less, 57% or less, 55% or less, 52% or less, 50% or less, 47% or less, 45% or less, 42% or less, 40% or less, or 37% by weight. It may be contained in an amount of % or less. Preferably, the baking soda may be contained in an amount of 30 to 70% by weight based on the total weight of the composition.

If the baking soda content is less than 10% by weight, the effect of inhibiting biofilm formation is reduced, and if it is more than 70% by weight, taste preference and formulation stability are reduced due to the unique pungent taste.

According to one embodiment of the present invention, the L-menthol may be contained in an amount of 0.1 to 1% by weight based on the total weight of the composition. Specifically, the content of L-menthol is 0.1% by weight, 0.14% by weight, 0.18% by weight, 0.2% by weight, 0.24% by weight, 0.28% by weight, 0.3% by weight, 0.34% by weight, based on the total weight of the composition. Weight% or more, 0.38 weight% or more, 0.4 weight% or more, 0.45 weight% or more, 0.5 weight% or more, 0.55 weight% or more, 0.6 weight% or more, 0.65 weight% or more, 0.7 weight% or more, 0.75 weight% or more, 0.8 weight% or more It may be more than 1% by weight, more than 0.85% by weight, more than 0.9% by weight, or more than 0.95% by weight, and the content of L-menthol is less than 1% by weight, less than 0.95% by weight, less than 0.9% by weight, and less than 0.85% by weight based on the total weight of the composition. Weight% or less, 0.8 weight% or less, 0.75 weight% or less, 0.7 weight% or less, 0.65 weight% or less, 0.6 weight% or less, 0.55 weight% or less, 0.5 weight% or less, 0.45 weight% or less, 0.4 weight% or less, 0.38 weight% or less It may be less than 0.34% by weight, less than 0.3% by weight, less than 0.28% by weight, less than 0.24% by weight, less than 0.2% by weight, less than 0.18% by weight, or less than 0.14% by weight.

If the content of L-menthol is less than 0.1% by weight or more than 1% by weight, there is a disadvantage in that the taste preference of the composition is reduced.

According to one embodiment of the present invention, the composition may include both sodium saccharin and beta-cyclodextrin.

At this time, according to one embodiment of the present invention, for the desired effect, the sodium saccharin may be contained in an amount of 0.1 to 1% by weight based on the total weight of the composition. Specifically, the content of sodium saccharin is 0.1% by weight, 0.14% by weight, 0.18% by weight, 0.2% by weight, 0.24% by weight, 0.28% by weight, 0.3% by weight, 0.34% by weight, based on the total weight of the composition. % or more, 0.38 weight% or more, 0.4 weight% or more, 0.45 weight% or more, 0.5 weight% or more, 0.55 weight% or more, 0.6 weight% or more, 0.65 weight% or more, 0.7 weight% or more, 0.75 weight% or more, 0.8 weight % or more, 0.85 wt% or more, 0.9 wt% or more, or 0.95 wt% or more, and the content of sodium saccharin is 1 wt% or less, 0.95 wt% or less, 0.9 wt% or less, and 0.85 wt% based on the total weight of the composition. or less, 0.8 weight% or less, 0.75 weight% or less, 0.7 weight% or less, 0.65 weight% or less, 0.6 weight% or less, 0.55 weight% or less, 0.5 weight% or less, 0.45 weight% or less, 0.4 weight% or less, 0.38 weight% or less Hereinafter, it may be 0.34 wt% or less, 0.3 wt% or less, 0.28 wt% or less, 0.24 wt% or less, 0.2 wt% or less, 0.18 wt% or less, or 0.14 wt% or less.

If the content of sodium saccharin is less than 0.1% by weight or more than 1% by weight, there is a disadvantage in that the taste preference of the composition is reduced.

Additionally, according to one embodiment of the present invention, the beta-cyclodextrin may be contained in an amount of 0.1 to 2% by weight based on the total weight of the composition. Specifically, the content of the beta-cyclodextrin is 0.1% by weight or more, 0.15% by weight or more, 0.2% by weight or more, 0.25% by weight or more, 0.3% by weight or more, 0.35% by weight or more, 0.4% by weight or more, 0.45% by weight or more, 0.5% by weight or more, 0.55% by weight or more, 0.6% by weight or more, 0.65% by weight or more, 0.7% by weight or more, 0.8% by weight or more, 0.9% by weight or more, 1% by weight or more, 1.2% by weight or more, It may be 1.4% by weight or more, 1.6% by weight or more, or 1.8% by weight or more, and the content of beta-cyclodextrin is 2% by weight or less, 1.8% by weight or less, 1.6% by weight or less, and 1.4% by weight or less based on the total weight of the composition. , 1.2% by weight or less, 1% by weight or less, 0.9% by weight or less, 0.8% by weight or less, 0.7% by weight or less, 0.65% by weight or less, 0.6% by weight or less, 0.55% by weight or less, 0.5% by weight or less, 0.45% by weight or less. , may be 0.4 wt% or less, 0.35 wt% or less, 0.3 wt% or less, 0.25 wt% or less, 0.2 wt% or less, or 0.15 wt% or less.

If the content of beta-cyclodextrin is less than 0.1% by weight or more than 2% by weight, there is a disadvantage in that the taste preference of the composition is reduced.

Additionally, according to one embodiment of the present invention, the weight ratio of L-menthol:saccharin sodium:beta-cyclodextrin may be 0.1 to 1:0.1 to 1:0.1 to 2. Within the range of the above weight ratio, the unique unpleasant taste of baking soda can be effectively improved.

According to one embodiment of the present invention, the composition may include all of sodium carboxymethyl cellulose, sodium alginate, and xanthan gum.

At this time, according to one embodiment of the present invention, the carboxymethyl cellulose sodium may be contained in an amount of 0.1 to 2% by weight based on the total weight of the composition. Specifically, the content of sodium carboxymethyl cellulose is 0.1% by weight or more, 0.15% by weight or more, 0.2% by weight or more, 0.25% by weight, 0.3% by weight or more, 0.35% by weight or more, 0.4% by weight or more, 0.45% by weight or more, 0.5% by weight or more, 0.55% by weight or more, 0.6% by weight or more, 0.65% by weight or more, 0.7% by weight or more, 0.8% by weight or more, 0.9% by weight or more, 1% by weight or more, 1.2% by weight or more, It may be 1.4% by weight or more, 1.6% by weight or more, or 1.8% by weight or more, and the content of sodium carboxymethyl cellulose is 2% by weight or less, 1.8% by weight or less, 1.6% by weight or less, and 1.4% by weight or less based on the total weight of the composition. , 1.2% by weight or less, 1% by weight or less, 0.9% by weight or less, 0.8% by weight or less, 0.7% by weight or less, 0.65% by weight or less, 0.6% by weight or less, 0.55% by weight or less, 0.5% by weight or less, 0.45% by weight or less. , may be 0.4 wt% or less, 0.35 wt% or less, 0.3 wt% or less, 0.25 wt% or less, 0.2 wt% or less, or 0.15 wt% or less.

If the content of sodium carboxymethyl cellulose is less than 0.1% by weight, formulation stability is reduced and it is inconvenient due to a sluggish feeling of use, and if it is more than 2% by weight, the viscosity increases too much and the feeling of use is poor.

Additionally, according to one embodiment of the present invention, the sodium alginate may be contained in an amount of 0.1 to 2% by weight based on the total weight of the composition. Specifically, the content of sodium alginate is 0.1% by weight, 0.15% by weight, 0.2% by weight, 0.25% by weight, 0.3% by weight, 0.35% by weight, 0.4% by weight, 0.45% by weight, based on the total weight of the composition. % or more, 0.5% by weight or more, 0.55% by weight or more, 0.6% by weight or more, 0.65% by weight or more, 0.7% by weight or more, 0.8% by weight or more, 0.9% by weight or more, 1% by weight or more, 1.2% by weight or more, 1.4% by weight % or more, 1.6 wt% or more, or 1.8 wt% or more, and the content of sodium alginate is 2 wt% or less, 1.8 wt% or less, 1.6 wt% or less, 1.4 wt% or less, 1.2 wt% based on the total weight of the composition. or less, 1% by weight or less, 0.9% by weight or less, 0.8% by weight or less, 0.7% by weight or less, 0.65% by weight or less, 0.6% by weight or less, 0.55% by weight or less, 0.5% by weight or less, 0.45% by weight or less, 0.4% by weight. Hereinafter, it may be 0.35% by weight or less, 0.3% by weight or less, 0.25% by weight or less, 0.2% by weight or less, or 0.15% by weight or less.

If the content of sodium alginate is less than 0.1% by weight, formulation stability is reduced and it is uncomfortable to use due to the feeling of being sluggish. If it is more than 2% by weight, the viscosity increases too much and the feeling of use is poor.

Additionally, according to one embodiment of the present invention, the xanthan gum may be contained in an amount of 0.1 to 1% by weight based on the total weight of the composition. Specifically, the content of xanthan gum is 0.1% by weight, 0.14% by weight, 0.18% by weight, 0.2% by weight, 0.24% by weight, 0.28% by weight, 0.3% by weight, 0.34% by weight, based on the total weight of the composition. % or more, 0.38 weight% or more, 0.4 weight% or more, 0.45 weight% or more, 0.5 weight% or more, 0.55 weight% or more, 0.6 weight% or more, 0.65 weight% or more, 0.7 weight% or more, 0.75 weight% or more, 0.8 weight % or more, 0.85 wt% or more, 0.9 wt% or more, or 0.95 wt% or more, and the content of xanthan gum may be 1 wt% or less, 0.95 wt% or less, 0.9 wt% or less, and 0.85 wt% based on the total weight of the composition. or less, 0.8 weight% or less, 0.75 weight% or less, 0.7 weight% or less, 0.65 weight% or less, 0.6 weight% or less, 0.55 weight% or less, 0.5 weight% or less, 0.45 weight% or less, 0.4 weight% or less, 0.38 weight% or less Hereinafter, it may be 0.34 wt% or less, 0.3 wt% or less, 0.28 wt% or less, 0.24 wt% or less, 0.2 wt% or less, 0.18 wt% or less, or 0.14 wt% or less.

If the content of xanthan gum is less than 0.1% by weight, formulation stability is reduced and it is uncomfortable to use due to the feeling of feeling sluggish, and if it is more than 1% by weight, the viscosity increases too much and the feeling of use is poor.

Additionally, according to one embodiment of the present invention, the weight ratio of sodium carboxymethyl cellulose:sodium alginate:xanthan gum may be 0.1 to 2:0.1 to 2:0.1 to 1. Within the range of the above weight ratio, the flowing feeling and syneresis phenomenon due to the high content of baking soda can be effectively improved, and the viscosity may not become excessively high.

The oral composition of the present invention is not particularly limited in its formulation, and may specifically have formulations such as toothpaste, oral cleaner, and tooth whitening agent.

In addition, the oral composition of the present invention may contain commonly used wetting agents, auxiliary foaming agents, pH adjusters, preservatives, medicinal ingredients, fragrances, brighteners, pigments, solvents, etc., depending on the formulation and purpose of use.

Hereinafter, the content of the present invention will be described in more detail through examples and test examples. However, these examples and test examples are only presented to understand the content of the present invention, and the scope of the present invention is not limited to these examples and test examples, and modifications and substitutions commonly known in the art and insertion, etc. can be performed, and this is also included in the scope of the present invention.

[Comparative Examples 1 to 25 and Examples 1 to 3]

The toothpastes of Comparative Examples 1 to 25 and Examples 1 to 3 were prepared using the compositions shown in Tables 1 to 3 below (unit: weight %).

Comparative example
One Comparative example
2 Comparative example
3 Comparative example
4 Comparative example
5 Comparative example
6 Comparative example
7 Comparative example
8 Comparative example
9 water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 sodium monofluorophosphate 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 cetylpyridinium chloride - - 0.05 - - - - - - Sodium saccharin 0.2 0.2 0.2 0.4 0.2 0.2 0.2 0.2 0.2 beta-cyclodextrin - - - - - 0.4 - - - glycerin 25 25 25 25 25 25 25 25 25 Spices One One One One One One One One One L-Menthol - - - - 0.2 - - - - Hydrated silica 10 3 3 3 3 3 3 3 5 sodium carboxymethyl cellulose One 0.5 0.5 0.5 0.5 0.5 - - - xanthan gum - - - - - - 0.5 - - sodium alginate - - - - - - - 0.5 - baking soda - 35 - 35 35 35 35 35 35 sodium lauryl sulfate 2 2 2 2 2 2 3 2 2

Comparative example
10 Comparative example
11 Comparative example
12 Comparative example
13 Comparative example
14 Comparative example
15 Comparative example
16 Comparative example
17 Comparative example
18 water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 sodium monofluorophosphate 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 cetylpyridinium chloride - - - - - - - - - Sodium saccharin 0.05 1.5 0.3 0.3 0.3 0.3 0.3 0.3 0.3 beta-cyclodextrin 0.4 0.4 0.05 2.5 0.4 0.4 0.4 0.4 0.4 glycerin 25 25 25 25 25 25 25 25 25 Spices One One One One One One One One One L-Menthol 0.2 0.2 0.2 0.2 0.05 1.5 0.2 0.2 0.2 Hydrated silica 3 3 3 3 3 3 3 3 3 sodium carboxymethyl cellulose 0.1 0.1 0.1 0.1 0.1 0.1 0.05 2.5 0.1 xanthan gum 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.2 0.05 sodium alginate 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 0.15 baking soda 35 35 35 35 35 35 35 35 35 sodium lauryl sulfate 2 2 2 2 2 2 2 2 2

Comparative example
19 Comparative example
20 Comparative example
21 Comparative example
22 Comparative example
23 Comparative example
24 Comparative example
25 Example
One Example
2 Example
3 water To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 To 100 Sodium monofluorophosphate
(sodium monofluorophosphate) 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 0.76 Cetylpyridinium chloride
(cetylpyridinium chloride) - - - - - - - - - - Sodium saccharin
(sodium saccharin) 0.3 0.3 0.3 0.3 0.3 0.3 0.2 0.3 0.3 0.6 beta-cyclodextrin
(beta-cyclodextrin) 0.4 0.4 0.4 0.4 0.4 0.4 - 0.4 0.4 0.6 glycerin 25 25 25 25 25 25 20 25 25 25 Spices One One One One One One One One One One L-Menthol 0.2 0.2 0.2 0.2 0.2 0.2 - 0.2 0.2 0.2 Hydrated silica 3 3 3 3 3 3 3 3 5 3 sodium carboxymethyl cellulose 0.1 0.1 0.1 0.1 0.1 One 0.1 0.1 0.7 0.1 xanthan gum 1.5 0.2 0.2 0.2 0.2 - 0.2 0.2 0.7 0.1 sodium alginate 0.15 0.05 2.5 0.15 0.15 - 0.15 0.15 0.3 0.15 baking soda 35 35 35 8 75 35 35 35 10 70 Sodium Lauryl Sulfate
(sodium lauryl sulfate) 2 2 2 2 2 2 2 2 2 2

[Test Example 1] Evaluation of toothpaste taste preference, formulation texture, and formulation stability

Comparative Examples 2 to 25 and Examples 1 to 3 containing baking soda or an antibacterial agent (cetylpyridinium chloride) were used by three expert panelists, and the overall taste preference and formulation texture were evaluated after repeated use three times ( ( In addition, formulation stability was evaluated by filling a glass bottle with toothpaste and storing it at 60°C for one week to observe whether the syneresis phenomenon, that is, the separation of the contents into water and solid, occurred. At this time, the viscosity was so high that it was difficult to use. Formulation stability was evaluated except for Comparative Examples 17, 19, and 21, which were evaluated as excessively high. The results are shown in Table 4 below.

Comparative example
2 Comparative example
3 Comparative example
4 Comparative example
5 Comparative example
6 Comparative example
7 Comparative example
8 Comparative example
9 Comparative example
10 Taste preference X X X X X X X X X FormulationTexture X X X X X X X X O Formulation stability separation separation separation separation separation separation separation separation stability Comparative Example 11 Comparative example
12 Comparative example
13 Comparative example
14 Comparative example
15 Comparative example
16 Comparative example
17 Comparative example
18 Comparative example
19 Taste preference X X X X X O O O O FormulationTexture O O O O O X X X X Formulation stability stability stability stability stability stability separation - separation - Comparative Example 20 Comparative example
21 Comparative example
22 Comparative example
23 Comparative example
24 Comparative example
25 Example
One Example
2 Example
3 Taste preference O O O X O X O O O FormulationTexture X X X X X O O O O Formulation stability separation - stability separation separation stability stability stability stability

From the results in Table 4, Comparative Examples 2 to 9 and 25 containing no at least one of beta-cyclodextrin and L-menthol, and sodium saccharin, beta-cyclodextrin, or L-menthol, respectively, in accordance with the present invention. In the case of Comparative Examples 10 to 15 containing the amount below the lower limit or above the upper limit of the range, it was confirmed that the taste preference was lowered.

In addition, Comparative Examples 2 to 9 and 24, which do not contain at least one of sodium carboxymethyl cellulose, sodium alginate, and xanthan gum, contain sodium carboxymethyl cellulose, sodium alginate, or xanthan gum below the lower limit or upper limit of the content range according to the present invention, respectively. In the case of Comparative Examples 16 to 21 containing excessive amounts, it was confirmed that the formulation was runny or the viscosity was excessively high.

In addition, in the case of Comparative Example 22 containing baking soda at 8% by weight, which is lower than 10% by weight, the taste preference was good, but the formulation texture was poor, and in the case of Comparative Example 22 containing baking soda at 75% by weight, which is higher than 70% by weight. In case 23, it was confirmed that both taste preference and formulation texture decreased.

Meanwhile, in the case of Comparative Examples 2 to 9 containing only one or none of sodium carboxymethyl cellulose, sodium alginate, and xanthan gum, a syneresis phenomenon was observed as early as the 3rd day when stored at 60°C, and carboxymethyl cellulose Comparative Examples 16, 18, and 20 containing sodium, sodium alginate, or xanthan gum in amounts below the lower limit of the content range according to the present invention, Comparative Example 23 containing 75% by weight of baking soda, and xanthan gum and sodium alginate It was confirmed that a syneresis phenomenon was observed even in Comparative Example 24, which did not contain any.

On the other hand, baking soda is contained in an amount of 10 to 70% by weight according to the present invention, and sodium saccharin, beta-cyclodextrin, L-menthol, sodium carboxymethyl cellulose, sodium alginate, and xanthan gum are contained within the content range according to the present invention. It was confirmed that Examples 1 to 3 showed excellent results in taste preference, formulation texture, and formulation stability.

[Test Example 2] Evaluation of the effect of inhibiting biofilm formation

An experiment was conducted to evaluate the effect of inhibiting biofilm formation for Comparative Examples 1 to 3, 22 to 25, and Examples 1 to 3. The procedure was specifically carried out using the tooth simulating material, Hydroxyapatite Disc (HD; Biotek), using the following method.

a) Streptococcus mutans (KCTC 3065) was inoculated into Brain Heart infusion broth (Difco) and incubated in a 35°C incubator (Imperial III Incubator, Lab Line Instruments Inc.) for 24 hours. Cultured.

b) The hydroxyapatite disk was sterilized by placing it in 70% ethanol for 30 minutes and then washed three times with sterilized deionized water.

c) Hydroxyapatite disks were placed one by one in a 24-well plate (BD Falcon) and completely dried.

d) Comparative Examples 1 to 3, 22 to 25, and Examples 1 to 3 were each diluted to 20%, and then 1 ml of each was dispensed into wells containing the hydroxyapatite disks and treated for 3 minutes.

e) The hydroxyapatite disk was washed with sterilized ionized water to remove the remaining sample.

f) After transferring the hydroxyapatite disk to another 24-well plate, 1 ml of brain heart leachate containing 1% sucrose was added to each well, 1 μl of cultured Streptococcus mutans culture medium was inoculated, and then incubated at 35°C for 3 days. Biofilm was formed by culturing for one day.

g) After 3 days, the hydroxyapatite disk was washed with sterilized ionized water, then treated with 1 ml of 0.1% crystal violet solution (Sigma), stained and washed for 30 minutes, dried completely at room temperature, and then washed with 70% ethanol. 1ml was added and the dye was extracted until the dyed hydroxyapatite disc was discolored to white.

h) After diluting the extract in steps of 1/2, the absorbance (Optical Density, 590 nm) was measured using a spectrophotometer (Spectramax 190, Molecular Devices Coporation).

i) The efficacy of inhibiting biofilm formation was calculated by subtracting the value of the blank well from the measured value and selecting the value of the dilution ratio measured as a value of 2 or less.

The results are shown in Table 5 below. When the biofilm formation inhibition effect (%) is more than 30%, it can be judged that there is biofilm formation inhibition effect.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 22 Comparative Example 23 Comparative Example 24 Comparative Example 25 Example 1 Example 2 Example 3 Biofilm inhibition effect (%) 20.91 48.12 49.24 28.91 52.12 48.39 48.13 48.77 32.6 50.61

From the results in Table 5, it was confirmed that the biofilm inhibition effect was significantly reduced in Comparative Example 1, which did not contain baking soda, and Comparative Example 22, which contained 8% by weight of baking soda. On the other hand, it was confirmed that Comparative Examples 2, 3, 23 to 25, and Examples 1 to 3, which contained 10% by weight or more of antibacterial agent or baking soda, had excellent efficacy in inhibiting biofilm formation.

At this time, when combined with the results of Test Example 1, Comparative Examples 2 and 3 containing 35% by weight of baking soda or an antibacterial agent have the ability to inhibit biofilm formation, but taste preference, feeling of use, and formulation stability are poor, and baking soda or antibacterial agents are suppressed. In the case of Comparative Examples 23 and 24, which contained 75% by weight and 35% by weight of soda, respectively, the feeling of use and formulation stability were poor, and although they contained 35% by weight of baking soda, they did not contain beta-cyclodextrin and L-menthol. In the case of Comparative Example 25, it can be seen that taste preference is reduced.

[Test Example 3] Toothpaste use feeling evaluation

Based on the results of evaluating the effect of inhibiting biofilm formation in Test Example 2, the feeling of use of toothpaste was evaluated for Comparative Example 2 and Examples 1 to 3. After using the toothpastes of Comparative Example 2 and Examples 1 to 3 repeatedly 3 times by 10 professional panel members who were trained to evaluate the feeling of use of toothpaste products for 3 months, they were evaluated for sharp taste, refreshing feeling, texture preference, and overall preference. After doing so, the results are shown in Table 6.

Comparative Example 2 Example 1 Example 2 Example 3 overall preference 3.1 6.6 7.1 5.3 Preference for spicy taste 1.6 7.4 8.4 6.6 refreshing 7.2 8.2 7.5 8.6 preference for texture 4.4 7.1 8.5 7.2

(1 point: very dissatisfied/ 3 points: dissatisfied/ 5 points: average/ 7 points: satisfied/ 9 points: very satisfied)

From the results in Table 6, in Examples 1 to 3 according to the present invention, the spicy taste and texture were significantly improved compared to Comparative Example 2, which did not contain beta-cyclodextrin, L-menthol, xanthan gum, and sodium alginate. It was confirmed that the refreshing feeling, which is a characteristic of baking soda, was also higher in Examples 1 to 3 of the present invention compared to Comparative Example 2.

That is, in the case of Examples 1 to 3 according to the present invention, it was confirmed that the unique unpleasant taste of baking soda was effectively improved, and the biofilm inhibition effect was excellent while having excellent taste preference, feeling of use, and formulation texture.

Claims (12)

baking soda;
L-menthol;
sodium saccharin and beta-cyclodextrin; and
Contains one or more selected from sodium carboxymethyl cellulose, sodium alginate, and xanthan gum;
Containing the baking soda in an amount of 10 to 70% by weight based on the total weight of the composition,
The weight ratio of L-menthol:saccharin sodium:beta-cyclodextrin is 0.1 to 1:0.1 to 1:0.1 to 2 in an oral composition. According to paragraph 1,
An oral composition, characterized in that it contains the L-menthol in an amount of 0.1 to 1% by weight based on the total weight of the composition. delete According to paragraph 1,
An oral composition, characterized in that it contains the sodium saccharin in an amount of 0.1 to 1% by weight based on the total weight of the composition. According to paragraph 1,
An oral composition, characterized in that it contains the beta-cyclodextrin in an amount of 0.1 to 2% by weight based on the total weight of the composition. delete According to paragraph 1,
The composition is an oral composition, characterized in that it contains all of sodium carboxymethyl cellulose, sodium alginate, and xanthan gum. In clause 7,
An oral composition, characterized in that it contains the sodium carboxymethyl cellulose in an amount of 0.1 to 2% by weight based on the total weight of the composition. In clause 7,
An oral composition, characterized in that it contains the sodium alginate in an amount of 0.1 to 2% by weight based on the total weight of the composition. In clause 7,
An oral composition, characterized in that it contains xanthan gum in an amount of 0.1 to 1% by weight based on the total weight of the composition. In clause 7,
A composition for oral use, wherein the weight ratio of sodium carboxymethyl cellulose:sodium alginate:xanthan gum is 0.1 to 2:0.1 to 2:0.1 to 1. The oral composition according to any one of claims 1, 2, 4, 5, and 7 to 11, wherein the composition is for suppressing biofilm formation.