KR102603185B1 - Cosmetic composition comprising the extract of Spiraea salicifolia L. - Google Patents
Cosmetic composition comprising the extract of Spiraea salicifolia L. Download PDFInfo
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- KR102603185B1 KR102603185B1 KR1020220087427A KR20220087427A KR102603185B1 KR 102603185 B1 KR102603185 B1 KR 102603185B1 KR 1020220087427 A KR1020220087427 A KR 1020220087427A KR 20220087427 A KR20220087427 A KR 20220087427A KR 102603185 B1 KR102603185 B1 KR 102603185B1
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- cosmetic composition
- spirea
- lanceolate
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- 239000000284 extract Substances 0.000 title claims abstract description 41
- 239000000203 mixture Substances 0.000 title claims abstract description 23
- 239000002537 cosmetic Substances 0.000 title claims abstract description 22
- 241000681535 Spiraea salicifolia Species 0.000 title 1
- 230000037303 wrinkles Effects 0.000 claims abstract description 12
- 241001092387 Spiraea Species 0.000 claims description 25
- 230000000694 effects Effects 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000000638 solvent extraction Methods 0.000 claims description 4
- 125000004432 carbon atom Chemical group C* 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 8
- 238000000605 extraction Methods 0.000 description 7
- 210000003491 skin Anatomy 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 230000003833 cell viability Effects 0.000 description 5
- 239000000654 additive Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 3
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 229930003268 Vitamin C Natural products 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 3
- 235000019154 vitamin C Nutrition 0.000 description 3
- 239000011718 vitamin C Substances 0.000 description 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
- 238000000134 MTT assay Methods 0.000 description 2
- 231100000002 MTT assay Toxicity 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000001153 anti-wrinkle effect Effects 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000263 cytotoxicity test Toxicity 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 239000000049 pigment Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 239000012679 serum free medium Substances 0.000 description 2
- 230000009759 skin aging Effects 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229940058015 1,3-butylene glycol Drugs 0.000 description 1
- DKPFZGUDAPQIHT-UHFFFAOYSA-N Butyl acetate Natural products CCCCOC(C)=O DKPFZGUDAPQIHT-UHFFFAOYSA-N 0.000 description 1
- 239000012981 Hank's balanced salt solution Substances 0.000 description 1
- 241000721662 Juniperus Species 0.000 description 1
- 241001098499 Lanceolata Species 0.000 description 1
- 235000017879 Nasturtium officinale Nutrition 0.000 description 1
- 240000005407 Nasturtium officinale Species 0.000 description 1
- 235000003823 Petasites japonicus Nutrition 0.000 description 1
- 240000003296 Petasites japonicus Species 0.000 description 1
- 241000218657 Picea Species 0.000 description 1
- 241000218594 Picea pungens Species 0.000 description 1
- 241001104043 Syringa Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 235000019437 butane-1,3-diol Nutrition 0.000 description 1
- 235000001436 butterbur Nutrition 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- -1 cleansing Substances 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 238000002784 cytotoxicity assay Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 235000011187 glycerol Nutrition 0.000 description 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 231100001083 no cytotoxicity Toxicity 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000006072 paste Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 235000013772 propylene glycol Nutrition 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 230000037394 skin elasticity Effects 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Mycology (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Cosmetics (AREA)
Abstract
본 발명은 피부 주름 개선 효과가 우수한 꼬리조팝나무 추출물을 포함하는 화장료 조성물에 관한 것이다. The present invention relates to a cosmetic composition containing a lanceolate extract that is excellent in improving skin wrinkles.
Description
본 발명은 피부 주름 개선 효과가 우수한 꼬리조팝나무 추출물을 포함하는 화장료 조성물에 관한 것이다. The present invention relates to a cosmetic composition containing a lanceolate extract that is excellent in improving skin wrinkles.
노화, 스트레스, 환경 등의 요인에 의해 피부 탄력의 저하나 잔주름 발생과 같은 피부의 노화가 진행되고, 특히, UVA 자외선은 피부 세포를 손상시켜 피부 주름 생성을 유도한다. 이러한 피부 노화를 방지 또는 지연시키고자 하는 수요자의 요구가 강하고, 최근 이러한 현상은 성별이나 연령대를 불문하고 증가하고 있다. 이에 항노화, 주름 개선, 탄력 개선에 우수한 효과를 갖는 성분을 포함하는 화장품에 대한 연구 개발이 활발히 진행되고 있다. Factors such as aging, stress, and the environment cause skin aging, such as a decrease in skin elasticity and the development of fine wrinkles. In particular, UVA ultraviolet rays damage skin cells and induce the formation of skin wrinkles. There is a strong demand from consumers to prevent or delay skin aging, and this phenomenon has recently been increasing regardless of gender or age group. Accordingly, research and development is being actively conducted on cosmetics containing ingredients that have excellent effects on anti-aging, wrinkle improvement, and elasticity improvement.
일례로, 등록특허 10-1323487은 물냉이, 주니퍼, 백출 및 머위의 추출물 중 어느 하나 이상을 유효 성분으로 포함하는 항주름제를 개시하고 있다. 이외에도 다양한 식물 추출물을 유효 성분으로 포함하는 항주름 효과를 지닌 화장료 조성물이 제안되고 있으나, 종래의 화장품은 UVA 자외선에 대한 세포 보호를 통해 피부 주름을 개선하기에 충분한 효과를 나타내지 못하고 있다. For example, Registered Patent No. 10-1323487 discloses an anti-wrinkle agent containing one or more of the extracts of watercress, juniper, Baekchul, and butterbur as an active ingredient. In addition, cosmetic compositions with anti-wrinkle effects containing various plant extracts as active ingredients have been proposed, but conventional cosmetics do not show a sufficient effect to improve skin wrinkles through cell protection against UVA ultraviolet rays.
본 발명은 피부 주름 개선 효과가 우수한 꼬리조팝나무 추출물을 포함하는 화장료 조성물을 제공한다. The present invention provides a cosmetic composition containing a lanceolate extract that is excellent in improving skin wrinkles.
본 발명의 화장료 조성물은 꼬리조팝나무 추출물을 포함한다.The cosmetic composition of the present invention includes a lanceolate extract.
본 발명은 피부 주름 개선 효과가 우수한 꼬리조팝나무 추출물을 포함하는 화장료 조성물을 제공한다. 본 발명에 따른 화장료 조성물은 UVA 자외선에 대한 세포 보호 효능이 우수하고, 그 결과 우수한 피부 주름 개선 효과를 발휘한다.The present invention provides a cosmetic composition containing a lanceolate extract that is excellent in improving skin wrinkles. The cosmetic composition according to the present invention has an excellent cell protection effect against UVA ultraviolet rays, and as a result, it exhibits an excellent skin wrinkle improvement effect.
도 1은 꼬리조팝나무 추출물의 세포독성 시험 결과이다.
도 2는 꼬리조팝나무 추출물의 UVA 손상 개선 효과를 나타낸 그래프이다.
도 3은 조팝나무 추출물의 UVA 손상 개선 효과를 나타낸 그래프이다.Figure 1 shows the results of a cytotoxicity test of a lanceolate extract.
Figure 2 is a graph showing the UVA damage improvement effect of the lanceolate extract.
Figure 3 is a graph showing the UVA damage improvement effect of spirea extract.
이하, 본 발명에 대하여 설명한다. 그러나, 하기 내용에 의해서만 한정되는 것은 아니며, 필요에 따라 각 구성요소가 다양하게 변형되거나 선택적으로 혼용될 수 있다. 따라서, 본 발명의 사상 및 기술범위에 포함되는 모든 변경, 균등물 내지 대체물을 포함하는 것으로 이해되어야 한다.Hereinafter, the present invention will be described. However, it is not limited to the following content, and each component may be variously modified or selectively mixed as needed. Accordingly, it should be understood to include all changes, equivalents, and substitutes included in the spirit and technical scope of the present invention.
본 발명의 화장료 조성물은 꼬리조팝나무 추출물을 포함한다. 또한, 본 발명의 화장료 조성물은 항산화제, 안정화제, 비타민, 안료, 향료, 방부제 등 해당 기술분야에서 통상 사용되는 첨가제를 더 포함할 수 있다.The cosmetic composition of the present invention includes a lanceolate extract. In addition, the cosmetic composition of the present invention may further include additives commonly used in the art, such as antioxidants, stabilizers, vitamins, pigments, fragrances, and preservatives.
상기 꼬리조팝나무 추출물은 꼬리조팝나무, 예를 들어 꼬리조팝나무의 잎, 줄기, 뿌리 및 꽃으로 이루어진 군에서 선택되는 하나 이상으로부터 얻은 추출물을 포함한다. The spirea extract includes an extract obtained from one or more species selected from the group consisting of leaves, stems, roots and flowers of spirea, for example, spirea.
상기 꼬리조팝나무를 세척하고 건조한 후, 해당 기술분야에 공지된 추출 방법을 사용하여 추출물을 제조할 수 있다. 예를 들어, 상기 꼬리조팝나무 추출물은 용매 추출법, 열수 추출법, 이산화탄소에 의한 감압 및 고온에 의한 초임계 추출법, 초음파 추출법 등으로 제조할 수 있고, 일례로, 용매 추출법으로 제조할 수 있다.After washing and drying the spirea, the extract can be prepared using an extraction method known in the art. For example, the syringa extract can be prepared by solvent extraction, hot water extraction, supercritical extraction using reduced pressure and high temperature using carbon dioxide, ultrasonic extraction, etc. For example, it can be prepared by solvent extraction.
상기 용매 추출법에 사용되는 추출 용매는 물, 탄소수 1 내지 4의 무수 또는 함수 저급 알코올, 프로필렌글리콜, 1,3-부틸렌글리콜, 글리세린, 아세톤, 디에틸에테르, 에틸아세테이트, 부틸아세테이트, 디클로로메탄, 클로로포름, 헥산 및 이들의 혼합물로 이루어진 군에서 선택되는 하나 이상의 용매일 수 있다. 일례로, 상기 추출 용매는 탄소수 1 내지 4의 무수 또는 함수 저급 알코올일 수 있다. The extraction solvent used in the solvent extraction method is water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, 1,3-butylene glycol, glycerin, acetone, diethyl ether, ethyl acetate, butyl acetate, dichloromethane, It may be one or more solvents selected from the group consisting of chloroform, hexane, and mixtures thereof. For example, the extraction solvent may be an anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms.
꼬리조팝나무와 용매는 1 : 5 내지 50 중량비, 예를 들어 1 : 5 내지 25 중량비, 다른 예로 1 : 10 내지 20 중량비로 혼합될 수 있다. The spirea and the solvent may be mixed at a weight ratio of 1:5 to 50, for example, 1:5 to 25, and for example, 1:10 to 20.
상기 추출은 통상의 온도 및 압력의 조건 하에서 수행될 수 있고, 일례로, 상온, 대기 중에서 수행될 수 있다. 또한, 상기 추출은 통상의 침지 시간 및 추출 횟수로 수행될 수 있고, 일례로, 2 내지 4일간 침지하는 추출 과정을 2 내지 4회 반복할 수 있다. 상기와 같이 추출된 수득물을 감압 농축한 후, 동결 건조(예를 들어, 5 Torr, 72 hr)하여 추출물을 제조할 수 있다. The extraction may be performed under normal temperature and pressure conditions, for example, at room temperature and in the air. Additionally, the extraction may be performed using normal immersion times and extraction times. For example, the extraction process of immersion for 2 to 4 days may be repeated 2 to 4 times. The extract can be prepared by concentrating the extracted product as described above under reduced pressure and then freeze-drying (e.g., 5 Torr, 72 hr).
상기 꼬리조팝나무 추출물은 UVA 자외선에 대한 세포 보호 효능이 우수하고, 그 결과 우수한 피부 주름 개선 효과를 발휘한다.The lanceolate extract has an excellent cell protection effect against UVA ultraviolet rays, and as a result, it exhibits an excellent skin wrinkle improvement effect.
화장료 조성물 총 중량을 기준으로, 상기 꼬리조팝나무 추출물을 0.005 내지 30 중량% 포함할 수 있다. 꼬리조팝나무 추출물의 함량이 전술한 범위 미만인 경우 주름 개선 효과가 미미할 수 있고, 전술한 범위를 초과하는 경우 경제성이 저하될 수 있다.Based on the total weight of the cosmetic composition, the extract may contain 0.005 to 30% by weight. If the content of the spirea extract is less than the above-mentioned range, the wrinkle improvement effect may be minimal, and if it exceeds the above-mentioned range, economic feasibility may be reduced.
본 발명의 화장료 조성물은 항산화제, 안정화제, 비타민, 안료, 향료, 방부제 등 해당 기술분야에서 통상 사용되는 첨가제를 더 포함할 수 있다. 상기 첨가제는 필요에 따라 해당 기술분야에서 통상 사용되는 양을 포함할 수 있으며, 일례로, 화장료 조성물 총 중량을 기준으로, 상기 첨가제를 각각 0.1 내지 10 중량% 포함할 수 있다. The cosmetic composition of the present invention may further include additives commonly used in the art, such as antioxidants, stabilizers, vitamins, pigments, fragrances, and preservatives. The additives may include amounts commonly used in the relevant technical field as needed. For example, the additives may each include 0.1 to 10% by weight based on the total weight of the cosmetic composition.
본 발명의 화장료 조성물은 해당 기술분야에서 통상적으로 제조되는 어떠한 제형으로도 제조될 수 있으며, 예를 들어, 용액, 현탁액, 유탁액, 페이스트, 겔, 크림, 로션, 파우더, 비누, 클렌징, 오일 등으로 제형화될 수 있으나, 이에 한정되는 것은 아니다. The cosmetic composition of the present invention can be prepared in any formulation commonly manufactured in the relevant technical field, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing, oil, etc. It may be formulated as, but is not limited to this.
이하, 실시예를 통하여 본 발명을 보다 구체적으로 설명한다. 그러나, 하기 실시예는 본 발명의 이해를 돕기 위한 것일 뿐 어떠한 의미로든 본 발명의 범위가 실시예로 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. However, the following examples are only intended to aid understanding of the present invention and do not limit the scope of the present invention to the examples in any way.
[제조예: 꼬리조팝나무 추출물 제조] [Manufacturing example: Manufacture of lanceolata extract]
꼬리조팝나무를 세척하고 건조한 후, 줄기 및 뿌리로 구분하였다. 꼬리조팝나무의 줄기 30 g을 메탄올 450 g에 투입하고, 3일씩 3회 반복 상온 침지하였다. 상기 추출된 수득물을 감압 농축한 후, 동결 건조(5 Torr, 72 hr)하여, 꼬리조팝나무 뿌리 추출물 4.02 g을 수득하였다. After washing and drying the spirea, it was divided into stems and roots. 30 g of the stem of spirea spruce was added to 450 g of methanol, and immersed at room temperature three times for three days each. The extracted product was concentrated under reduced pressure and then freeze-dried (5 Torr, 72 hr) to obtain 4.02 g of C. spruce root extract.
꼬리조팝나무의 뿌리를 사용한 것을 제외하고는 상기와 동일한 방법으로 꼬리조팝나무 줄기 추출물 3.05 g을 수득하였다.3.05 g of spirea stem extract was obtained in the same manner as above, except that the roots of lanceolate lanceolate were used.
[비교제조예: 조팝나무 추출물 제조] [Comparative manufacturing example: Manufacturing of spirea extract]
조팝나무를 사용한 것을 제외하고는, 제조예와 동일한 방법으로 조팝나무 뿌리 추출물 및 조팝나무 줄기 추출물을 제조하였다.A spirea root extract and a spirea stem extract were prepared in the same manner as in the preparation example, except that spirea vulgaris was used.
[실험예 1: 세포 독성 평가, MTT assay][Experimental Example 1: Cytotoxicity evaluation, MTT assay]
섬유아세포에 대한 꼬리조팝나무 추출물의 세포 독성을 확인하기 위해, MTT assay를 실시하였다. 96 well plate에 섬유아세포(CCD-986sk)를 5Х104 cells/mL의 농도로 배양하고, 24시간 후에 무혈청 배지에 제조예의 각 추출물을 0.4 내지 100 μg/mL 농도로 희석하여 처리하였다. 24시간 후, 5 mg/mL MTT(M2128, sigma)를 20 μL 넣어 3시간 배양하였다. 이후, 배지를 모두 제거하고, 150 μL DMSO를 각 well에 넣어 교반한 후, Microplate reader로 570 nm에서 흡광도를 측정하였다. 하기 식으로 세포 생존율을 계산하였고, 세포 생존율이 80 % 이상인 경우, 세포 독성이 없는 것으로 판단하였다. To confirm the cytotoxicity of the Spirea lanceolate extract on fibroblasts, MTT assay was performed. Fibroblasts (CCD-986sk) were cultured in a 96 well plate at a concentration of 5Х10 4 cells/mL, and after 24 hours, each extract from the preparation example was diluted to a concentration of 0.4 to 100 μg/mL in serum-free medium. After 24 hours, 20 μL of 5 mg/mL MTT (M2128, Sigma) was added and cultured for 3 hours. Afterwards, all medium was removed, 150 μL DMSO was added to each well, stirred, and the absorbance was measured at 570 nm using a microplate reader. Cell viability was calculated using the formula below, and when the cell viability was 80% or more, it was judged that there was no cytotoxicity.
세포 생존율(%) = 100 X (실험군 흡광도 / 대조군 흡광도)Cell viability (%) = 100
도 1에 나타난 바와 같이, 꼬리조팝나무 줄기 추출물 및 꼬리조팝나무 뿌리 추출물은 모두 100 μg/mL 농도 이하에서 무독성임을 확인할 수 있다. As shown in Figure 1, it can be confirmed that both the spirea stem extract and the spirea root extract are non-toxic at a concentration of 100 μg/mL or less.
[실험예 2: UVA 손상 개선 효능 평가, UVA Irradiation and Cytotoxicity Assay] [Experimental Example 2: Evaluation of UVA damage improvement efficacy, UVA Irradiation and Cytotoxicity Assay]
96 well plate에 섬유아세포(CCD-986sk)를 5Х104 cells/mL의 농도로 24시간 배양하였다. 배지를 제거하고 HBSS를 넣은 후, UV irradiation system(VILBER)을 이용하여 UVA 30 J/cm2 를 조사하였다. UVA 조사 종료 후, 무혈청 배지에 비타민 C, 제조예 및 비교제조예의 각 추출물을 100 μg/mL 농도로 희석하여 처리하였다. 21시간 후, 5 mg/mL MTT(M2128, sigma)를 10 μL 넣어 3시간 배양하였다. 이후, 배지를 모두 제거하고, 150 μL DMSO를 각 well에 넣어 교반한 후, Microplate reader로 570 nm에서 흡광도를 측정하고, 하기 식으로 세포 생존율을 계산하였다.Fibroblasts (CCD-986sk) were cultured in a 96 well plate at a concentration of 5Х10 4 cells/mL for 24 hours. After removing the medium and adding HBSS, UVA 30 J/cm 2 was irradiated using a UV irradiation system (VILBER). After completion of UVA irradiation, vitamin C and each extract of Preparation Examples and Comparative Preparation Examples were diluted to a concentration of 100 μg/mL and treated in serum-free medium. After 21 hours, 10 μL of 5 mg/mL MTT (M2128, Sigma) was added and cultured for 3 hours. Afterwards, all medium was removed, 150 μL DMSO was added to each well and stirred, absorbance was measured at 570 nm using a microplate reader, and cell viability was calculated using the following formula.
세포 생존율(%) = 100 X (실험군 흡광도 / 대조군 흡광도)Cell viability (%) = 100
도 2는 꼬리조팝나무 추출물의 UVA 손상 개선 효과를 나타낸 그래프이다. 도 2에 나타난 바와 같이, 본 발명에 따른 제조예의 꼬리조팝나무 줄기 추출물 및 꼬리조팝나무 뿌리 추출물로 처리한 경우, 무첨가군(blank) 대비 세포 생존율 증가를 나타내었으며, 비타민 C에 비하여 UVA 손상 개선 효과가 우수하게 나타났다. Figure 2 is a graph showing the UVA damage improvement effect of the lanceolate extract. As shown in Figure 2, when treated with the spirea stem extract and the spirea root extract of the preparation example according to the present invention, the cell survival rate was increased compared to the no addition group (blank), and the effect of improving UVA damage compared to vitamin C was shown. appeared excellent.
도 3은 조팝나무 추출물의 UVA 손상 개선 효과를 나타낸 그래프이다. 도 3에 나타난 바와 같이, 비교제조예의 조팝나무 줄기 추출물 및 조팝나무 뿌리 추출물로 처리한 경우, 무첨가군(blank)에 비해 세포 생존율이 증가하지 않았으며, 비타민 C에 비하여 UVA 손상 개선 효과가 낮게 나타났다. Figure 3 is a graph showing the UVA damage improvement effect of spirea extract. As shown in Figure 3, when treated with the spirea stem extract and the spirea root extract of the comparative preparation example, the cell survival rate did not increase compared to the no-added group (blank), and the effect of improving UVA damage was lower than that of vitamin C. .
상기 결과로부터, 본 발명에 따른 꼬리조팝나무 줄기 추출물 및 꼬리조팝나무 뿌리 추출물이 자외선에 대한 우수한 세포 보호 효능을 나타냄을 확인할 수 있다.From the above results, it can be confirmed that the spirea stem extract and the spirea root extract according to the present invention exhibit excellent cell protection efficacy against ultraviolet rays.
Claims (7)
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| PCT/KR2023/009765 WO2024014809A1 (en) | 2022-07-15 | 2023-07-10 | Cosmetic composition comprising spiraea salicifolia l. extract |
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| US20030190300A1 (en) * | 2002-04-05 | 2003-10-09 | Scancarella Neil D. | Cosmetic compositions containing meadowsweet extract and methods for treating skin |
| KR101323487B1 (en) | 2005-04-13 | 2013-10-31 | 가부시키가이샤 시세이도 | Antiwrinkle agent |
| KR20140043258A (en) * | 2012-09-24 | 2014-04-09 | 중앙대학교 산학협력단 | Novel hemiterpene glucoside compounds isolated from the leaves of spiraea prunifolia and anti-oxidative and anti-inflammatory use thereof |
| KR20190134211A (en) * | 2018-05-25 | 2019-12-04 | 주식회사 차메디텍 | A cosmetic composition for hair containing an extract of Spiraea prunifolia |
| KR20200080556A (en) * | 2018-12-27 | 2020-07-07 | 재단법인 홍천메디칼허브연구소 | Composition for preventing or treating skin disease comprising extract of Spiraea prunifolia simpliciflora |
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| KR20020073041A (en) * | 2001-03-14 | 2002-09-19 | 주식회사 에스티씨나라 | Composition of natural material for acne treatment and prevention |
| US20030190300A1 (en) * | 2002-04-05 | 2003-10-09 | Scancarella Neil D. | Cosmetic compositions containing meadowsweet extract and methods for treating skin |
| KR101323487B1 (en) | 2005-04-13 | 2013-10-31 | 가부시키가이샤 시세이도 | Antiwrinkle agent |
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| KR20190134211A (en) * | 2018-05-25 | 2019-12-04 | 주식회사 차메디텍 | A cosmetic composition for hair containing an extract of Spiraea prunifolia |
| KR20200080556A (en) * | 2018-12-27 | 2020-07-07 | 재단법인 홍천메디칼허브연구소 | Composition for preventing or treating skin disease comprising extract of Spiraea prunifolia simpliciflora |
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