KR102569581B1 - Functional bee feed composition and method for producing the same - Google Patents

Abstract

The present invention relates to a functional bee feed composition and a method for producing the same, in which high-quality honey with improved antioxidant and anti-inflammatory effects can be obtained through a bee feed applied with onions, which can be obtained relatively easily and cheaply, and a method for producing the same, By providing a bee feed that is a mixture of the filtrate obtained from water bath and raw onion juice mixed with honey and kelp extract, it is possible to obtain better antioxidant and anti-inflammatory effects than bee feed mixed with aronia, which is expected to have high functionality. By determining the composition ratio of the composition, it is possible to economically and stably provide bee feed for stable production of high-functional honey to ensure stable income of beekeepers.

Description

Functional bee feed composition and method for producing the same {Functional bee feed composition and method for producing the same}

The present invention relates to a functional bee feed composition and a method for producing the same, and more particularly, to a functional bee feed to which high-quality honey with improved antioxidant and anti-inflammatory effects can be obtained through a relatively easy and inexpensive bee feed containing onions. It relates to a bee feed composition and a manufacturing method thereof.

The medical effects of honey include bacterial inhibition, rapid use as metabolic energy, promotion of liver function and detoxification, and treatment of constipation by promoting peristalsis of the digestive tract.

Physiologically active substances contained in honey include flavonoids and phenolic acids, as well as various types of phenolic substances such as tannin and methyl syringate.

Since bees obtain necessary food from flowers and plants, store and use it, they can maintain it without feeding artificial food. As the beekeeping industry increases, food feeding becomes more urgent for the proliferation and maintenance of productive bee colonies.

Furthermore, it is necessary to study the value of produced honey when using useful natural substances for breeding and production, especially for the establishment of food preparation and feeding system in breeding management of bee colony.

As a study related to such artificial feeding of bees, studies are being conducted to increase the physiological activity of honey when manufacturing fermented honey. To develop a liquid sugar feed for bees using a mixture concentrate of sugars, water, albumin, enzymes, vitamins, salt, alpha soybean flour, etc. Research is also being conducted to manufacture functional honey by mixing it with feed.

As these studies progressed, studies were also conducted to obtain high-quality honey with increased antioxidant activity by mixing natural products with bee feed, and studies were conducted to feed green tea, plum, and chestnut flower extracts as liquid feed to formulated milk mills. In this case, the titratable acidity of the harvested honey was higher in the plum extract feed, the species flavonoid content was higher in the chestnut extract feed, and the total phenol content, SOD-like activity and electron donating ability were higher in the green tea extract.

In addition, when raspberry extract was added to the food of honey, there was also a study that the antioxidant activity of honey increased as functional ingredients such as polyphenol compounds and flavonoids contained in raspberry were transferred to honey.

However, in the case of such conventional bee feed containing natural substances, the effect is only at a level where the effect can be estimated to some extent in that the natural substance is an expensive material having excellent functionality in the first place.

On the other hand, in the case of aronia, which is known to be high in antioxidant and anticancer components and is in the spotlight as a new health supplement, it was confirmed through experiments that the antioxidant and anticancer functions can be enhanced by mixing the aronia extract with bee feed.

In the case of Aronia, it is a general term for shrubs and fruits belonging to the genus Rosaceae and Aronia, which were used as a panacea in medieval Europe. is getting However, these active ingredients of aronia are destroyed when heated, so they are used in the form of fresh fruit or powder, and are known to help prevent liver damage and relieve inflammation as well as provide excellent antioxidant and anticancer effects due to their high anthocyanin content.

However, in the case of aroina, it is cultivated as a special product in Danyang-gun and Yangju-si in Korea, but 90% of the world production is in Poland, and domestic production is in high demand as a health supplement, so using it as bee feed is economical or stable in terms of raw material supply and demand. There is a limit that is not effective in .

Korean Patent Registration No. 10-2080608 [Method for producing honey with high mineral and antioxidant effect]

An object of the present invention is to obtain high-quality honey with improved antioxidant and anti-inflammatory effects through a bee feed using onions, which can be obtained relatively easily and inexpensively.

In addition, the present invention provides a bee feed obtained by mixing the mixture of the filtrate obtained by boiling the onion and the raw onion juice with honey and kelp extract, which is superior in antioxidant and anti-inflammatory properties to bee feed mixed with aronia, which is expected to have high functionality. The purpose is to economically produce high-quality honey by determining the composition ratio of the bee feed composition that can obtain the effect.

The method for preparing a functional bee feed composition according to an embodiment of the present invention includes the steps of preparing an onion mixture by mixing an extract extracted by boiling onions and raw onion juice, preparing a kelp leachate, and adding honey to the kelp leachate. After preparing a bee feed base by mixing, preparing a bee feed composition by mixing the onion mixture with 30% of the total bee feed weight.

As an example, the onion mixture may be a mixture of an extract filtered by filtering onion at 80 to 85 degrees in a water bath for 30 minutes to 2 hours and a juice obtained by squeezing and filtering raw onion at a ratio of 9:1.

As an example, the kelp leachate is for mineral supplementation and may be a leachate extracted for 4 to 6 hours at room temperature by adding kelp to water at a ratio of 10:1.

As an example, the bee feed composition may be prepared from a liquid wheat source in which 65% by weight of honey, 5% by weight of kelp leachate, and 30% by weight of an onion mixture are mixed.

Functional bee feed composition according to another embodiment of the present invention is a combination of onion extract filtered by filter paper by boiling onion at 80 to 85 ° C for 30 minutes to 2 hours and raw onion juice filtered and mixed at a ratio of 9:1 It consists of 30% by weight of the mixture, 5% by weight of kelp extract obtained by adding kelp to water at a ratio of 10:1 and extracting it at room temperature for 4 to 6 hours, and 65% by weight of honey.

The functional bee feed composition and method for producing the same according to an embodiment of the present invention have the effect of continuously obtaining high-quality honey with improved antioxidant and anti-inflammatory effects through a bee feed using onion, which can be obtained relatively easily and inexpensively. .

In addition, the functional bee feed composition and method for producing the same according to an embodiment of the present invention provides a bee feed obtained by mixing a mixture of a filtrate obtained by boiling onions and raw onion juice with honey and kelp leachate, so that high functionality is expected. By determining the composition ratio of the bee feed composition that can obtain more excellent antioxidant and anti-inflammatory effects than bee feed mixed with aronia, stable income of beekeepers is provided by economically and stably providing bee feed for stable production of high-functional honey. There is a guaranteed effect.

1 is a flow chart of a method for producing a functional bee feed composition according to an embodiment of the present invention.
2 is a photograph showing an actual experimental process according to an embodiment of the present invention.
3 to 5 are graphs measuring antioxidant activity for several types of samples.
6 to 7 are graphs confirming the effect of samples on cancer cells using human-derived gastric cancer cells and breast cancer cells, respectively.
Figure 8 is a graph measuring the antihypertensive activity for several types of samples.
9 and 10 are graphs measuring anti-inflammatory activity (cytotoxicity and NO content) of samples.
Figure 11 is a graph measuring the hepatoprotective activity for several samples.

It should be noted that technical terms used in the present invention are only used to describe specific embodiments and are not intended to limit the present invention. In addition, technical terms used in the present invention should be interpreted in terms commonly understood by those of ordinary skill in the art to which the present invention belongs, unless specifically defined otherwise in the present invention, and are excessively inclusive. It should not be interpreted in a positive sense or in an excessively reduced sense. In addition, when the technical terms used in the present invention are erroneous technical terms that do not accurately express the spirit of the present invention, they should be replaced with technical terms that those skilled in the art can correctly understand. In addition, general terms used in the present invention should be interpreted as defined in advance or according to context, and should not be interpreted in an excessively reduced sense.

Also, singular expressions used in the present invention include plural expressions unless the context clearly dictates otherwise. Terms such as "consisting of" or "comprising" in the present invention should not be construed as necessarily including all of the various elements or steps described in the invention, and some of the elements or steps may not be included. It should be construed that it may, or may further include additional components or steps.

In addition, terms including ordinal numbers such as first and second used in the present invention may be used to describe components, but components should not be limited by the terms. Terms are used only to distinguish one component from another. For example, a first element may be termed a second element, and similarly, a second element may be termed a first element, without departing from the scope of the present invention.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings, but the same or similar components are given the same reference numerals regardless of reference numerals, and redundant description thereof will be omitted.

In addition, in describing the present invention, if it is determined that a detailed description of a related known technology may obscure the gist of the present invention, the detailed description will be omitted. In addition, it should be noted that the accompanying drawings are only for easily understanding the spirit of the present invention, and should not be construed as limiting the spirit of the present invention by the accompanying drawings.

Hereinafter, a functional bee feed composition and a manufacturing method according to the present invention will be described in detail with reference to the drawings.

1 is a flow chart of a method for producing a functional bee feed composition according to an embodiment of the present invention.

As shown, the step of preparing an onion mixture by mixing the extract extracted by boiling the onion and the raw onion juice is performed. Such an onion mixture can be prepared by mixing only pure onions in a water bath at 80 to 85 degrees for 30 minutes to 2 hours, filtering the extract and filtering the raw onion and filtering the juice at a ratio of 9:1.

On the other hand, a step of preparing a kelp leachate for mineral supplementation to bees is performed. This kelp leachate is for mineral supplementation and may be a leachate extracted for 4 to 6 hours at room temperature by adding kelp to water at a ratio of 10:1.

After preparing a bee feed base by mixing honey with the kelp leachate prepared through the above processes, a step of preparing a bee feed composition is performed by mixing an onion mixture with 30% of the total bee feed weight.

Here, the bee feed composition may be prepared from a liquid wheat source in which 65% by weight of honey, 5% by weight of kelp extract, and 30% by weight of an onion mixture are mixed.

Thereafter, the prepared bee feed composition is fed to honey and then harvested to obtain onion honey.

In addition to confirming whether the functional active ingredient of the bee feed composition produced according to the bee feed manufacturing method shown in FIG. 1 provides antioxidant and anti-inflammatory properties and no cytotoxicity, it is confirmed that the bee feed composition is a natural product with expensive antioxidant and anticancer properties. Experiments were conducted with various contents in order to compare the bee feed containing rhonia with the honey fed.

For the experiment, in the case of aroina and onion, 10% by weight, 30% by weight, and 50% by weight were mixed in a bee feed base mixed with kelp leachate and honey, respectively, and then prepared feed for feeding and tested twice. Results were averaged. On the other hand, if necessary, in order to compare with the effect obtained in the process of transitioning these components from the body of the bee to honey, a sample of honey mixed with onion or aronia is also compared.

Figure 2 is a photograph showing the process of preparing a bee feed mixed with aronia and onion in various mixing ratios for the experiment, as well as preparing a bee feed in the manner according to FIG. 1 and feeding it.

Afterwards, these bee feeds were supplied and the honey produced thereby was harvested, and these samples were tested, but for accurate component analysis and efficacy evaluation, polar substances (saccharides such as glucose and fructose, 98% by weight or more) and non-polar substances (phenols, flavonoids, etc.) Less than 2% by weight of the active ingredient) was separated and the experimental data was conducted only for non-polar materials.

Experiments were conducted on antioxidative activity, anti-inflammatory activity, antihypertensive activity, anticancer activity, and hepatoprotective activity, respectively.

First, total phenol content, DPPH radical scavenging activity and ABTS radical scavenging activity were measured as shown in FIGS. 3 to 5 in order to confirm antioxidant activity.

Figure 3 is a measurement of the total phenol content for several samples prepared in advance.

Phenolic compound is a general term for aromatic compounds with two or more phenolic hydroxyl (-OH) groups in one molecule. There are very many types, and the solubility according to each chemical structure is very diverse.

The total phenol content was measured by adding 200 μL of a 2% Na2CO3 solution to 10 μL of the sample and allowed to stand for 3 minutes. Then, 10 μL of 2 N Folin-Ciocalteu phenol reagent was added and mixed, and then the color was developed in an incubator at 30 ° C for 27 minutes. The absorbance of the colored sample was measured at 750 nm using a microplate reader (SpectraMax M5, Molecular Devices, CA, USA). The total phenol content was calculated using a standard calibration curve using gallic acid.

As shown, the polyphenol content tended to be higher in the production honey fed with aronia and onions compared to general honey, especially in 30% aronia fed honey, 206.31 ± 6.69 (GAE mg / g), onion 30 It can be seen that % feeding honey shows a high polyphenol content as 211.3±7.03 (GAE mg/g).

That is, it can be seen that the case of using the onion 30%-containing bee feed according to the embodiment of the present invention has the highest phenol content in terms of total phenol content.

Figure 4 is a measurement of DPPH radical scavenging activity for several samples.

DPPH radical scavenging activity is an antioxidant activity index for phenolic compounds, using the fact that DPPH, a relatively safe free radical with a dark purple color, is reduced and decolored by aromatic amines, antioxidants, polyhydroxy aromatic compounds, etc. It is used to measure the antioxidant activity. In the examples of the present invention, DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity was measured by 10 uL of the sample in a 96-well plate and 0.2 mM DPPH solution 190 prepared immediately before use. After mixing uL, the mixture was reacted for 30 minutes in a dark room at room temperature, and the absorbance was measured at 520 nm using a microplate reader (SpectraMax M5, Molecular Devices, CA, USA). Ascorbic acid was used for comparison as a positive control group, and the DPPH radical scavenging activity was calculated as [1-(absorbance of sample added / absorbance of negative control)] × 100 and expressed as a percentage.

As shown, the DPPH radical scavenging activity tended to be higher in the production honey fed with aronia and onion than in general honey, especially in 30% aronia fed honey and 84.66±1.03% in 30% onion fed honey. By showing high DPPH radical scavenging activity at 81.88±1.37%, it can be seen that onion 30% feed honey provides almost the same level of effect as Aronia.

5 is a measurement of ABTS radical scavenging activity for several samples.

The ABTS radical scavenging activity can be confirmed by measuring that the ABTS free radical generated by the reaction with potassium persulfate is removed by the antioxidant power in the sample and decolorized from bluish green to light green, a unique color of the radical. In the embodiment of the present invention, ABTS [2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid)] cation radical scavenging activity was obtained by mixing 2.45 mM potassium persulfate in a 7 mM ABTS solution at a ratio of 1:1 and then leaving it in the dark at room temperature for 24 hours. After that, ABTS radical (ABTS+·) was made and diluted with PBS (phosphate buffer saline, pH 7.4) buffer so that the absorbance value at 732 nm was 0.7±0.02. (ABTS: 4 tablets + DW 10mL/ PBS　: 1 tablet + DW 200mL)

In addition, 190 uL of ABTS radical solution and 10 uL of sample were added to a 96-well plate, allowed to stand for 1 minute, and then absorbance was measured at 732 nm. Ascorbic acid was used as a positive control group, and the ABTS radical scavenging activity was calculated as [1-(absorbance of sample added / absorbance of negative control)] × 100 and expressed as a percentage.

As shown, ABTS radical scavenging activity tended to be higher in production honey fed aronia and onion than in general honey, especially in 30% aronia fed honey and 97.05±0.28% in onion 30% fed honey. It can be seen that the ABTS radical scavenging activity was as high as 96.8±1.59%. It can also be seen that onion 30% feeding honey provides almost the same level of effect as aronia.

The following FIGS. 6 to 7 confirm the effect of samples on cancer cells using human-derived gastric cancer cells and breast cancer cells, respectively.

Cancer cells were human-derived cells, gastric cancer cells (AGS cells) and breast cancer cells (MCF-7 cells) were used, and DMEM medium containing 10% FBS and 1% streptomycin/penicillin was used at 37°C and 5% CO2. was cultured in an incubator where When the number of cells reached 80-90% in a 175-cm2 flask, the monolayer of cells was washed with phosphate buffered saline solution (PBS), subcultured using trypsin-EDTA, and the medium was changed every 2-3 days.

In order to observe the cancer cell proliferation inhibitory effect according to the sample, the cells were dispensed in a 96 well plate at 2 × 104 cells/mL and stabilized for 24 hours. Then, each cell was treated with 0, 50, 100, and 200 μg/mL of the sample, respectively, and cultured for 24 hours. After 24 hours, remove the medium, treat each well with 20 μL of MTS solution dissolved in PBS, incubate in a CO2 incubator for 3 hours, and measure the absorbance at 490 nm with an ELISA reader (Bio-Rad, Hercules, CA, USA). did

6 is a measurement of the degree to which various types of samples inhibit the growth of human-derived gastric cancer cells (AGS).

As shown, as the concentration of the functional honey extract increases according to the amount of onion and aronia feeding, a significant effect of inhibiting the survival of AGS gastric cancer cells can be confirmed. At 500 ug/mL, control groups 1 (regular honey) and 2 (specified honey) showed lower cancer cell inhibitory effects than the sample group at 18.28±0.15% and 16.10±0.21%, respectively. ± 0.23%) and onion 50% feed honey (12.80 ± 0.19%) showed a low cancer cell survival rate, showing a high AGS gastric cancer cell growth inhibitory effect, and onion 30% feed honey also showed a cancer cell suppression effect compared to the control. Able to know.

7 is a measurement of the degree to which various types of samples inhibit the growth of breast cancer cells (MCF-7).

As shown, as the concentration of the functional honey extract increased according to the amount of onion and aronia feeding, a significant effect of inhibiting the survival of MCF-7 breast cancer cells was confirmed. Control group (regular honey) showed 28.31±1.16 ~ 96.99±1.65%, 30% aronia feed honey (23.99±0.69 ~ 78.04±1.33%) and onion 30% feed honey (22.44±0.61 ~ 79.99±0.28 %) showed a low cancer cell survival rate, indicating that MCF-7 had a high effect on breast cancer cell growth inhibition.

Figure 8 is a measurement of antihypertensive activity for several types of samples.

The inactive form of angiotensin I present in the body is converted to angiotensin II, which has a blood vessel wall constriction effect, as the dipeptide is shed by ACE (Angiotensin-converting enzyme), and blood pressure rises by inactivating bradykinin, a blood pressure lowering factor in vivo. The activity can be known by checking the degree of inhibition of ACE, which is one of the causes of blood pressure increase in the bioregulatory function.

In the examples of the present invention, measurement was performed using ACE kit-WST (Dojindo Molecular Technologies, Inc., Kumamoto, Japan). Substrate buffer and enzyme working solution were added to 10 uL of the sample and reacted at 37°C for 1 hour. After putting the indicator working solution and reacting at room temperature for 10 minutes, the absorbance was measured at 450 nm. The negative control group was treated with distilled water instead of the sample, and the ACE inhibitory activity was expressed as a percentage (%).

As shown, the ACE inhibitory activity tended to be higher in production honey fed with aronia and onion than in general honey, especially in 30% aronia fed honey and 69.77 ± 0.75% in onion 30% fed honey. It can be seen that it exhibits a high ACE inhibitory activity at 67.92±1.19%.

9 and 10 are measurements of anti-inflammatory activity (cytotoxicity and NO content) of the samples.

To characterize anti-inflammatory activity, RAW264.7 cells, a mouse macrophage cell line, were cultured in DMEM (Dulbecco's modified eagle medium) medium supplemented with 10% FBS (fetal bovine serum) and 1% P/S (penicilin-streptomycin). was used. When the cultured RAW264.7 cells became 80% confluent, they were washed with PBS, detached using a cell scraper, centrifuged at 3,000 rpm for 4 minutes, and subcultured at 2-day intervals.

In order to confirm cytotoxicity, RAW 264.7 cells were dispensed in a 96-well plate to be 2 × 104 cells/well, and then pre-incubated in a CO2 incubator (37°C, 5% CO2) for 24 hours, and then each sample was prepared at the final concentration. (10, 50, 100, 200, 500 ug/mL) the cells were treated and cultured for 18 hours. Afterwards, 20 uL of MTS solution (5-(3-carboxymethoxyphenyl)-2H-tetraza lium inner salt) was added per well, reacted for 3 hours in a CO2 incubator, and then read at 490 °C using a microplate reader (SpectraMax M5, Molecular Devices, CA, USA). Absorbance was measured in nm.

On the other hand, the concentration of NO is divided into 2 × 104 cells / well in a 96-well plate and cultured in a CO2 incubator (37 ° C, 5% CO2) for 24 hours, and then each sample is 10, 50, 100, 200 ug After pre-treatment for 2 hours at a concentration of /mL, LPS (100 ng/mL) was treated and cultured for 18 hours. After adding the same amount of Griess reagent as the culture medium, absorbance was measured at 540 nm using a microplate reader (SpectraMax M5, Molecular Devices, CA, USA). The concentration of NO was calculated based on the standard curve for each concentration of NaNO2.

9 is a measurement of cytotoxicity for various samples.

As shown, the cell survival rates for Raw 264.7 cells found by treating functional honey extracts by concentration (10, 50, 100, 200, 500 ug/mL) by ratio of onion and aronia are as follows. When the survival rate of the control group (1: normal honey, 2: specification honey) not treated with the extract was set to 100%, the cell survival rate was less than 80%, which is the minimum concentration that does not cause cytotoxicity at a concentration of 500 ug / mL. Able to know. In the experiment of the present invention, if the sample processing concentration of the extract by ratio of onion and aronia is 200 ug / mL or less, it is judged that there will be no significant effect on the result of the experiment, and then the experiment is performed at a processing concentration of 200 ug / mL or less proceeded.

10 is a measurement of NO content for several samples.

As shown, after two hours of treatment with 10 to 200 ug / mL of functional honey extract by ratio of onion and aronia, cells were treated with 100 ng / mL of LPS, an inflammation-inducing substance, for 18 hours. As a result, LPS was treated. The NO concentration of 17.89±0.99 uM was shown in the group, and all groups showed higher NO production inhibition ability than the LPS-treated group. When treated with 200 ug/mL, in the group added with aronia, 10% feeding honey (8.75 ± 0.69 ~ 15.14 ± 0.75 uM), 30% feeding honey (9.39 ± 0.35 ~ 14.51 ± 0.84 uM), 50% Feed honey (9.64±0.52 ~ 14.87±0.61), 10% feed honey (9.11±0.30 ~ 14.26±0.17 uM), 30% feed honey (9.56±0.21 ~ 14.68±0.54 uM) in the onion-added group , showed the NO content of 50% fed honey (8.16±0.52 ~ 13.42±0.29). It can be seen that, when treated with 200 ug/mL, the functional honey extracts fed with 10% aronia and 30% onion showed the highest NO production inhibitory ability.

Figure 11 is the measurement of hepatoprotective activity for several samples.

For hepatocytes (BNL.CL2), DMEM (Dulbecco's modified eagle medium) supplemented with 10% fetal bovine serum (FBS) and 1% penicilin-streptomycin (P/S) was used. When the cultured BNL.CL2 cells became 80% confluent, they were washed with PBS, detached using a cell scraper, centrifuged at 3,000 rpm for 4 minutes, and subcultured at 2-day intervals.

The cultured BNL.CL2 cells were divided into 5 × 104 cells/24-well and cultured for 24 hours, then the medium was removed, washed twice with phosphate-buffered saline (PBS), and 1 mL of the extract was added for 24 hours. cultured for a while. Thereafter, the cells were treated with 0.4mM H ₂ O ₂ to cause oxidative stress, incubated for 2 hours, added with MTS solution, reacted at 37° C. for 2 hours, and cell viability was obtained by measuring absorbance at a wavelength of 450 nm.

As shown, functional honey extracts by ratio of onion and aronia were pretreated by concentration (10, 50, 100, 200 ug/mL) and then treated with 0.4 mM H ₂ O ₂ for 2 hours, H ₂ O ₂ The survival rate of the cells treated with was 47.14±0.82%, but when the functional honey extract was treated at a concentration of 200 ug/mL, 30% aronia fed honey (89.51±2.40%) and onion 30% fed honey (86.07 ±0.93%) showed a high survival rate. This shows that cell survival is improved by about 40% compared to cells treated with H ₂ O ₂ without pretreatment of functional honey, and the protective effect on liver cells in functional honey fed by adding 30% of aronia and 30% of onion is significant. can know that It can be seen that the sample expressed as +n% is a direct mixture of aronia or onion extract with honey, and has a significantly lower effect than that provided as bee feed.

In this way, in the case of a bee feed formulated with 30% of the total weight of the bath-bathed onion and raw onion mixture according to an embodiment of the present invention, it is the same as the case of applying aronia, an expensive functional natural product, even though inexpensive and easy-to-obtain onions are used. It can be seen that it shows better functionality, and it can be seen that it provides very excellent antioxidant, anti-cancer, anti-inflammatory and hepatoprotective effects compared to existing honey that does not apply these natural products.

The foregoing may be modified and modified by those skilled in the art without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain, and the scope of the technical idea of the present invention is not limited by these embodiments. The protection scope of the present invention should be construed according to the claims below, and all technical ideas within the equivalent range should be construed as being included in the scope of the present invention.

Claims (5)

Preparing an onion mixture by mixing the onion at 80 to 85 degrees Celsius for 30 minutes to 2 hours in a water bath for 30 minutes to 2 hours and mixing the extract filtered with filter paper and the filtered juice by squeezing raw onion at a weight ratio of 9: 1; Preparing a kelp extract by adding kelp at a weight ratio of 10:1 and extracting at room temperature for 4 to 6 hours;
A method for producing a functional bee feed composition comprising the step of preparing a bee feed composition with a liquid wheat source by mixing 65% by weight of honey, 5% by weight of the kelp leachate, and 30% by weight of the onion mixture.
delete delete delete 30% by weight of an onion mixture made by mixing the onion in a water bath at 80 to 85 degrees Celsius for 30 minutes to 2 hours and filtering the extract with filter paper and the filtered juice by squeezing raw onion at a ratio of 9:1, water and kelp at a ratio of 10:1 A functional bee feed composition consisting of 5% by weight of kelp extract extracted at room temperature for 4 to 6 hours and 65% by weight of honey.