KR102566894B1 - Cosmetic composition having anti-oxidant, anti-inflammatory and skin whiening effects which comprise DKMEX - Google Patents

Abstract

The present invention relates to a cosmetic composition containing DKMEX as an active ingredient and having antioxidant, anti-inflammatory, and whitening effects, and more particularly, comprising DKMEX as an active ingredient, wherein the DKMEX is used as an active ingredient in donkey oil, Lamiaceae, green tea, quince and situation It is characterized by mixing and extracting mushrooms at a weight ratio of 0.1 to 20.0%, respectively.
The cosmetic composition according to the present invention provides skin antioxidant effects, exhibits skin anti-inflammatory and whitening effects, and has the advantage of not causing skin irritation and side effects.

Description

Cosmetic composition having anti-oxidant, anti-inflammatory and skin whiening effects which comprise DKMEX}

The present invention relates to a cosmetic composition containing 'DKMEX' (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus phellitus) as an active ingredient and having antioxidant, anti-inflammatory and whitening activities.

Our human body is in close contact with the external environment, and among them, the skin is a body part directly exposed to the external environment, and is easily sensitized by environmental pollution, harmful substances, stress, and irregular lifestyles. The skin exposed to these external environments generates active oxygen, that is, free radicals, and the generated active oxygen and free radicals attack normal cell tissues or cell membranes to destroy cells and eventually cause inflammation in the skin. The inflamed tissue destroys proteins or genetic materials by the physiological mechanism of the skin and becomes a major cause of skin troubles as well as atopic dermatitis and skin aging. Increased free radicals destroy collagen, elastin, and hyaluronic acid, which are the connective tissue of the dermis, causing subsidence (wrinkles) in certain areas of the skin, and also oxidizes the lipid portion of the cell membrane to improve cell health. Destruction occurs and causes diseases such as dermatitis, acne, and skin cancer.

In addition, when the skin is overexposed to ultraviolet rays, melanin synthesis is promoted in melanosomes by the action of tyrosinase, and skin aging progresses rapidly. and cause skin cancer.

In order to improve the problems of these skin conditions, it is important to improve the surrounding living environment first, but various methods are needed to normalize the skin that has lost its function incidentally.

Korean Patent Laid-open Publication No. 10-2018-0031937 discloses prior art related to the anti-inflammatory effect, which has anti-itching and anti-inflammatory effects including goji berry, sasangja, tosaja, bokbunja, Schisandra chinensis, Cassia tora, cinnamon, and white gulchae extract. It relates to a cosmetic composition having.

However, the prior art is a cosmetic composition for skin improvement containing natural substances, which only exhibits an anti-inflammatory effect, but has a problem in that antioxidant and whitening activities are impossible.

Therefore, a cosmetic composition having antioxidant, anti-inflammatory and whitening activities is urgently required, and it is also necessary to use the excellent synergistic effect of extract mixtures of natural substances with fewer side effects on the human body.

Republic of Korea Patent Publication No. 10-2018-0031937 (published on March 29, 2018), "Cosmetic composition for skin improvement having anti-inflammatory effect"

Accordingly, an object of the present invention relates to a cosmetic composition having antioxidant, anti-inflammatory and whitening activities containing DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus phellitus) as an active ingredient.

In order to achieve the above object, it provides a cosmetic composition having antioxidant, anti-inflammatory and whitening activities, characterized in that it contains the present DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus phellitus) as an active ingredient.

In addition, the DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus) is a mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus Phellinus 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : It is characterized in that it is mixed in a weight ratio of 0.1 to 20.0.

In addition, the extract is characterized by extraction using any one or a mixture of two or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol.

In addition, the cosmetic composition is a lotion, toner, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake foundation, two-way cake, or powder pact It is characterized in that it is formulated into a dosage form.

It is to provide antioxidant, anti-inflammatory and whitening effects containing 'DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus)' according to the present invention as an active ingredient.

1 is a graph evaluating cell viability for each sample treatment concentration according to the present invention.
Figure 2 is a graph evaluating the radical scavenging effect for each sample treatment concentration according to the present invention;
Figure 3 is a graph evaluating NO production inhibition according to the sample treatment concentration according to the present invention.
Figure 4 is a graph evaluating the tyrosinase activity inhibitory effect by sample treatment concentration according to the present invention.
Figure 5 is a graph evaluating the melanin synthesis inhibition rate for each sample treatment concentration according to the present invention.

The detailed description of the present invention below refers to the accompanying drawings shown as examples of embodiments in which the present invention can be practiced. These embodiments are described in detail so that those skilled in the art will be able to practice the present invention. It should be understood that the various embodiments of the present invention are different, but need not be mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in one embodiment in another embodiment without departing from the spirit and scope of the invention. Additionally, it should be understood that the location or arrangement of individual components within each described embodiment may be changed without departing from the spirit and scope of the invention.

Accordingly, the detailed description set forth below is not to be taken in a limiting sense, and the scope of the present invention, if properly described, is limited only by the appended claims, along with all equivalents as claimed by those claims. Like reference numbers in the drawings indicate the same or similar function throughout the various aspects.

The terms used in the present invention have been selected from general terms that are currently widely used as much as possible while considering the functions in the present invention, but these may vary depending on the intention of a person skilled in the art or precedent, the emergence of new technologies, and the like. In addition, in a specific case, there is also a term arbitrarily selected by the applicant, and in this case, the meaning will be described in detail in the description of the invention. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, not simply the name of the term.

In the present invention, when a part "includes" a certain component, it means that it may further include other components, not excluding other components, unless otherwise stated.

The present invention provides a cosmetic composition containing 'DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus)' as an active ingredient and having antioxidant, anti-inflammatory and whitening activities.

The "Donkey milk" is milk most similar to human milk in terms of protein profile and lactose content, and its efficacy includes promotion of calcium absorption in the intestine, strengthening of the immune system, antioxidant, anti-aging, and antibacterial activity. In addition, donkey milk contains a large amount of essential amino acids, whey protein, unsaturated fatty acids, calcium, and selenium, and has low cholesterol and casein content, so it is rich in nutritional value (Ling Li et al., Korean Society of Food Science, 2018, 27(2), p393-400)

The "Prunella vulgaris" is a perennial plant belonging to the Lamiaceae family and is also called Hagocho. The stem of Lamiaceae is square, the flowers are purple, and the young leaves are used for food, and the outpost is mainly used for medicinal purposes. The outpost contains caffeic acid, ursolic acid, organic acid, etc. as active ingredients, and based on these ingredients, Lamiaceae extract is known to have antioxidant activity, anti-aging, antibacterial, and anti-inflammatory effects (Korean medicinal crops such as Hong Soo-young). Society, 2014, 22(2), p295-296).

The "green tea (Camellia Sinensis L.)" is a perennial seed plant belonging to the Camellia family, and refers to a product in which oxidizing enzymes are inactivated by drying and heating young leaves. Components of green tea include caffeine, tannins, vitamins A, B1, B2, C, and E, and representative physiologically active substances include catechins. In addition, it has pharmacological properties such as antioxidant, antibacterial, anticancer, inhibition of melanin production, diuresis and detoxification, and has been used in natural beverages and skin care from the past (Sung Ki-cheon, Korean Oil Painting Society, 2006, 23(2), p115-124) .

The "quince (Chaenomeles Sinensis)" is a mature fruit of a quince tree belonging to the Rosaceae family, and has a strong sour and astringent taste, making it difficult to reproduce. Quince contains saponin, malic acid, tartaric acid, citric acid, vitamin C, flavonoids, tannins, etc., and has pharmacological effects such as relief of cough and phlegm and promotion of digestive secretion (Lee Dae-hyung et al., 2002, 17(3) , p266-270).

The "Phellinus linteus" is a white rotting fungus belonging to Hymenochaetaceae and Phellinus taxonomically, and is called situation because it is yellow in all parts except for the cap surface. Phellinus Phellinus contains β-glucan, which has a side branch of β(1→6) bond to β(1→3) main bond. It has various physiological activities such as (Eunji Son et al., 2018, 61(1), p101-108).

In the present invention, 'DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus)' has excellent antioxidant activity, and a mixture of at least two of the above extracts, preferably 'DKMEX (donkey oil, Lamiaceae, Green tea, Chinese quince and Phellinus Phellinus extract)' was found to have an excellent synergistic effect.

In particular, 'DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus)' was used as an active ingredient to impart antioxidant, anti-inflammatory and skin-whitening effects.

According to a preferred embodiment of the present invention, the mixing ratio of the raw materials extracted by mixing the present invention 'DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus mushroom)' in equal amounts may be included in the following ratio.

'DKMEX' can be composed of mixed extracts of donkey milk, honey grass, green tea, quince and Phellinus Phellinus in a weight ratio of 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0, respectively.

In the cosmetic composition having antioxidant, anti-inflammatory and skin whitening effects containing 'DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus)' according to the present invention as an active ingredient, the mixed extract contains at least 0.01 to 30.0% by weight, more preferably 0.1 to 20.0% by weight, more preferably 1.0 to 10.0% by weight.

When the content of the mixed extract is less than 0.001% by weight, the antioxidant effect does not appear, and when it is contained at 30% by weight or more, the effect does not increase significantly, and problems arise in the safety and stability of the formulation, and thus the economic efficiency is generally lowered.

Antioxidant cosmetic composition according to the present invention is a combination based on scientific basic data on skin, and when 'DKMEX (mixed extract of donkey oil, honey grass, green tea, quince and Phellinus linteus mushroom)' is used as an active ingredient, it has antioxidant, anti-inflammatory and skin whitening activities. In terms of practical long-term efficacy can be realized.

Ingredients included in the cosmetic composition of the present invention include ingredients commonly used in cosmetic compositions other than 'DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus)' according to the present invention, such as antioxidants , conventional adjuvants such as stabilizers, solubilizers, vitamins, pigments, colors and flavors, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , Oil, powder foundation, emulsion foundation, wax foundation and spray may be formulated, but is not limited thereto. More specifically, it may be formulated into a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder formulation.

When the formulation of the present invention is a paste, cream or gel, any one or more of animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component can be used by selecting

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butyl Len glycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic esters, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquiolate, paraffin, Sorbitan stearate, lipophilic monostearic acid glycerin, stearic acid, glyceryl stearate/PEG-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, steareth-4, dicetylphosphate , Macadamia oil, carboxyvinyl polymer, xanthan gum, or sorbitan fatty acid ester may be selected and used.

When the formulation of the present invention is a suspension, water, ethanol, glycerin, liquid diluents such as butylene glycol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester and Same suspending agent, microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate , sorbitan sesquioleate, glyceryl monostearate/glyceryl stearate/polyoxyethylene stearate, wax, paraffin, squalane, caprylic/capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or tra Any one or more of the Kants may be selected and used.

When the formulation of the present invention is a surfactant-containing cleanser, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Any one or more of ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be selected and used.

In addition, the use efficacy of the cosmetic composition according to an embodiment of the present invention can be appropriately adjusted according to the individual's skin condition and condition, and external environmental conditions.

Hereinafter, examples and experimental examples will be described in detail to aid understanding of the present invention. However, the following examples and experimental examples are only provided to more easily understand the present invention, and should not be construed as limiting the technical level and scope of the present invention. Examples and experimental examples of the present invention are provided to more completely explain the present invention to those skilled in the art.

<Example 1> Preparation of donkey milk extract

Donkey milk was lyophilized and pulverized to obtain a pulverized product. 1 kg of an aqueous solution was added to 30% ethanol to make 50 g/L of pulverized donkey milk, extracted under reflux three times for 4 hours at 60 to 90 ° C, cooled to room temperature, and filtered using Whatman #3 filter paper. After filtering, concentration under reduced pressure and freeze-drying at less than 50 ° C., a donkey milk extract was prepared.

<Example 2> Preparation of Lamiaceae Extract

Lamiaceae was washed with flowing purified water, dried, and then pulverized to obtain a pulverized product. 1 kg of an aqueous solution of 30% ethanol was added to the pulverized Lamiaceae to 50 g/L, extracted under reflux three times for 4 hours at 60 to 90 ° C, cooled to room temperature, and then filtered with Whatman #3 filter paper. Lamiaceae extract was prepared by filtering, concentrating under reduced pressure and freeze-drying at 50 ° C or less.

<Example 3> Preparation of green tea extract

Green tea was washed with flowing purified water, dried, and then pulverized to obtain a pulverized product. 1 kg of an aqueous solution of 30% ethanol was added to the pulverized green tea to 50 g/L, extracted under reflux three times for 4 hours at 60 to 90 ° C, cooled to room temperature, and then filtered with Whatman #3 filter paper. Filtered, concentrated under reduced pressure and freeze-dried at 50 ° C or less to prepare a green tea extract.

<Example 4> Preparation of quince extract

The quince was washed in purified water flowing, dried, and then all pulverized to obtain a pulverized product. Add 1 kg of an aqueous solution to 30% ethanol to 50 g/L of the pulverized quince, extract under reflux three times for 4 hours at 60 ~ 90 ° C, cool to room temperature, and then filter paper using Whatman #3 filter paper. Filtered, concentrated under reduced pressure and freeze-dried at 50 ° C or less to prepare a quince extract.

<Example 5> Preparation of Sanghwang mushroom extract

Phellinus Phellinus were washed in purified water, dried, and then ground to obtain a ground product. 1 kg of an aqueous solution of 30% ethanol was added to the ground Phellinus Phellinus to 50 g/L, extracted under reflux three times for 4 hours at 60 to 90 ° C, cooled to room temperature, and filtered using Whatman #3 filter paper. It was filtered, concentrated under reduced pressure and freeze-dried at 50 ° C or less to prepare an extract of Phellinus linteus.

<Example 6> Mixed extract

1 kg of a 50% aqueous solution of ethyl alcohol was added to 50 g of the pulverized products obtained by pulverization as in Examples 2 to 5 mixed at a weight ratio of 1: 1: 1: 1, and 3 at 60 to 90 ° C for 4 hours each. After extraction under reflux and cooling to room temperature, the mixture was filtered with Whatman #3 filter paper, concentrated under reduced pressure and freeze-dried at 50° C. or less to prepare Mixed Extract A.

< Example 7 > DKMEX

Donkey milk, Lamigrass, green tea, quince and Phellinus Phellinus pulverized raw materials obtained by pulverization as in Examples 1 to 5 were mixed in a weight ratio of 1: 1: 1: 1: 50 g in 50% ethyl alcohol aqueous solution 1 kg was added, extracted under reflux three times for 4 hours at 60 ~ 90 ° C, cooled to room temperature, filtered with Whatman #3 filter paper, concentrated under reduced pressure and freeze-dried at 50 ° C or less to obtain mixed extract B was manufactured.

< test example 1 > Cell viability test

1) Experiment method

The effect on cell proliferation was confirmed with respect to the samples prepared in Examples 1 to 7. In order to confirm cell proliferation and toxicity, experiments were conducted in the following manner.

Cultured cells 3T3 (fibroblast cell line) were dispensed in a 96-well microplate at 5,000 cells/well and cultured in a thermostat for 30 minutes, and the samples were 0.0008, 0.0016, 0.0048, 0.008, 0.0112, 0.0114 ( %, W/V)) and cultured for 72 hours.

After 72 hours of culture, thiazoline blue was administered and additional culture was performed for 4 hours. After discarding all the culture solution, a reaction stop solution was added to each well of the microplate, stirred for 5 minutes, and absorbance was measured at 570 nm. For the control group, 10% fetal bovine serum (FBS) medium was administered as much as the amount of sample injected, and co-cultivation was performed under the optimal conditions for cell growth. did

The cell proliferation effect was calculated by the following formula 1, and the results are shown in Table 2 below.

2) Experimental results

sample name Cell viability (%) by sample treatment concentration (μg/ml) 0 44 88 264 308 352 Example 1 100 102.5 106.8 111.6 110.1 108.4 Example 2 100 100.2 99.8 99.7 94.2 89.5 Example 3 100 101.1 102.3 105.3 104.9 103.4 Example 4 100 100.8 100.2 99.4 97.4 93.1 Example 5 100 100.3 100.4 100.1 96.7 91.5 Example 6 100 100.8 101.5 100.3 110.2 91.8 Example 7 100 100.8 102.2 103.6 104.1 103.1

As shown in Table 1 above, as a result of confirming the cytotoxicity of donkey oil, Lamigrass, green tea, quince and Phellinus linteus mushroom extracts and mixed extracts extracted by mixing them, DKMEX prepared as in Examples 1 to 7, Example 1, In 3 and 7, it was confirmed that there was no cytotoxicity. On the other hand, it was confirmed that examples 2, 4, 5, and 6 showed some cytotoxicity.

<Test Example 2> Confirmation of free radical scavenging effect

1) Experiment method

The effect on free radical scavenging was confirmed for the samples prepared in Examples 1 to 7. In order to confirm the effect on free radical scavenging, an experiment was conducted in the following manner.

Experiments were performed using the DPPH (1,1-diphenyl-2picryl-hydrazyl) method (reference: Blois.M.S.Nature 181, 1190, 1958), and DPPH and Quercetin were used from SIGMA. . In 1 ml of 0.2 mM DPPH methanol solution, 2 ml each of Examples 1 to 7 and the positive control at various concentrations (8, 16, 48, 80 ppm) were added, stirred, and reacted at room temperature for 10 minutes. Then, the absorbance was measured at 517 nm, and at this time, purified water was used instead of each sample as a blank test.

Changes in free radicals were measured using the following formula 2, and the results are shown in Table 2.

2) Experimental results

The results of free radical scavenging are shown in Table 2 below.

sample name Radical scavenging effect (%) by sample treatment concentration (μg/ml) 44 88 264 704 100 control
(Ascorbic acid) 89.2 Example 1 26.2 41.9 65.1 83.4 One Example 2 13.6 30.2 49.6 69.8 2 Example 3 15.4 36.7 56.7 75.4 3 Example 4 14.8 32.4 53.4 71.5 4 Example 5 20.7 35.5 52.1 70.6 5 Example 6 22.6 39.6 61.8 76.2 6 Example 7 28.3 45.1 71.9 89.4 7

As shown in Table 2, it was confirmed that all of Examples 1 to 7 were effective in scavenging free radicals. In particular, in the case of Examples 1 and 7, it was confirmed that the antioxidant effect was significantly higher than that of Examples 2 to 6, and among them, the effect was the most excellent in Example 7.

<Test Example 3> Investigation of anti-inflammatory effect by inhibition of NO production

1) Experiment method

Raw 264.7 cells, which are mouse macrophages, were dispensed into a 6-well plate at a concentration of 3.5 Х 10 ⁵ cells/well and cultured for 24 hours. Thereafter, each well was replaced with a cell medium containing diluted samples prepared in Examples 1 to 7. As a stimulus, 1 μg of LPS (Lipopolysaccharide) was treated and cultured for 24 hours. 100 μl of the supernatant was transferred to a 96-well plate, 100 μl of Griess solution was added and reacted at room temperature for 10 minutes, and the NO inhibitory effect was determined by measuring the absorbance at 540 nm, and the results are shown in Table 3 below. showed up

sample name Inhibition of NO production (%) according to sample treatment concentration (μg/ml) 0 44 88 264 704 LPS (1 μg/ml) + + + + + Example 1 0 3.1 6.2 17.8 33.3 Example 2 0 0.8 2.3 11.6 24.1 Example 3 0 1.5 3.9 13.2 27.1 Example 4 0 1.5 4.2 13.4 27.9 Example 5 0 2.3 3.1 11.9 25.6 Example 6 0 3.1 5.4 14.7 29.5 Example 7 0 4.7 10.1 22.5 37.2

As shown in Table 3, it was confirmed that all Examples 1 to 7 had an effect of inhibiting NO production in a concentration-dependent manner. In particular, Examples 1 and 7 showed the same tendency as Experimental Examples 2, 3 and 4, and in particular, as in Example 7, DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus) was used as an active ingredient It was confirmed that the NO production inhibition rate was the best when containing as.

<Test Example 4> Inhibition of tyrosinase activity

1) Experiment method

The Tyrosinase activity inhibition experiment was performed to confirm the melanin activity inhibitory effect of the substance, and was measured by applying a method such as Yagi, which measures DOPA chrome by a colorimetric method.

In this experiment, a reaction solution was prepared by adding 40 μl of a 1.5 mM L-Tyrosine solution to 20 μl of 0.1 M phosphate buffer pH6.5 in a 96-well plate. After adding 10 μl and reacting for 5 minutes, 10 μl of mushroom tyrosinase (100 unit/ml) was added, and an enzyme reaction was performed at 37° C. for 10 minutes. After 10 minutes, dopachrome generated in the reaction solution was measured at 490 nm using a microplate counter.

Tyrosinase activity inhibition rate was calculated by the following formula 3, and the results are shown in Table 4 below.

2) Experimental results

sample name Tyrosinase activity inhibitory effect (%) by sample treatment concentration (μg/ml) 0 44 88 264 704 Example 1 0 5.4 12.3 17.4 25.5 Example 2 0 3.3 8.7 10.2 14.8 Example 3 0 4.8 11.6 16.5 19.1 Example 4 0 4.1 10.5 13.7 17.4 Example 5 0 3.6 9.4 12.1 15.7 Example 6 0 5.2 7.3 15.2 21.6 Example 7 0 5.8 13.2 19.1 27.3

As shown in Table 4, it was confirmed that the inhibition rate gradually increased in a concentration-dependent manner in all Examples 1 to 7, and as in Examples 1 and 7, donkey oil alone or DKMEX containing it (donkey oil, Lamiaceae, green tea, quince and Sangjae In the case of mushroom mixture extract), it was confirmed that the effect was increased compared to Examples 2 to 6. In particular, when mixed as in Example 7, it can be seen that it is most effective in inhibiting tyrosinase enzyme activity, exhibiting a synergistic effect in which the effect is further increased.

<Test Example 5> Inhibition of melanin synthesis

1) Experiment method

B16F10 mouse melanoma cells were dispensed in a 6-well plate at a concentration of 3.5 Х 10 ⁵ cells/well and cultured for 24 hours, and each well was treated with α-MSH (melanocyte stimulating hormone) and the sample prepared for each concentration. After culturing for 48 hours, the culture medium was removed by washing twice with phosphate buffer saline (PBS), and the attached cells were transferred to an EP tube and centrifuged to obtain a cultured cell pellet. The shape of the precipitated B16F10 melanoma cells was photographed, and the melanin was dissolved in DMSO-added 1N NaOH solution at 60 ° C. for 1 hour, and then the absorbance was measured at 405 nm by dispensing 200 μl into a 96-well plate.

The melanin synthesis inhibition rate was calculated by the following formula 4, and the results are shown in Table 5 below.

2) Experimental results

sample name Melanin synthesis inhibition rate (%) by sample treatment concentration (μg/ml) 0 44 88 264 704 α-MSH
(100 nM) + + + + + Example 1 0 5.8 13.9 24.1 27.8 Example 2 0 3.7 5.8 15.7 14.3 Example 3 0 5.4 8.4 20.5 21.1 Example 4 0 4.4 7 18.3 18.32 Example 5 0 4.1 6.6 24.1 16.1 Example 6 0 5.5 10.8 18.4 21.7 Example 7 0 7.4 19.1 27.2 32.9

As shown in Table 5, it was confirmed that the melanin synthesis inhibition rate increased in a concentration-dependent manner in all Examples 1 to 7. In particular, when donkey milk alone or DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Phellinus linteus) was used as in Examples 1 and 7, it was confirmed that the melanin synthesis inhibition rate was superior to that of Examples 2 to 6.

Hereinafter, based on the results of the above experimental examples, a cosmetic composition containing DDKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus) is formulated and presented as follows.

However, the composition of the present invention is not limited to the following formulation examples.

< formulation example 1 > DKMEX (donkey milk, honey grass, green tea, quince) and mixed extract of Sanghwang mushroom) as an active ingredient.

Example 7 The prescription examples of lotion (skin lotion) among cosmetics containing DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus) are shown in Table 6.

number Raw material Content (% by weight) One Example 7 DKMEX (mixed extract of donkey milk, honey grass, green tea, quince and Phellinus linteus) 5.0 2 glycerin 3.0 3 Butylene Glycol 2.0 4 propylene glycol 2.0 5 Polyoxyethylene Hydrogenated Castor Oil 1.0 6 ethanol 10.0 7 triethanolamine 0.1 8 antiseptic a very small amount 9 pigment a very small amount 10 Spices a very small amount 11 Purified water a very small amount

<Manufacturing method> Add No. 2, 3, 4, and 8 to No. 11 in order and stir to dissolve. After dissolving No. 5 by heating to about 60 ° C, add No. 10 to dissolve and then add No. 11. Finally, 6, 7, and 9 are added, but 1 is added, sufficiently stirred, and then aged.

< formulation example 2 > DKMEX (donkey milk, honey grass, green tea, quince) and mixed extract of Phellinus linteus) as an active ingredient.

Example 7 A prescription example of a nutritional lotion among cosmetics containing DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus) is shown in Table 7.

number Raw material Content (% by weight) One Example 7 DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Sanghwang mushroom) 5.0 2 beeswax 1.0 3 Polysorbate 60 1.5 4 Sorbitan Sesquioleate 0.5 5 liquid paraffin 10.0 6 Sorbitan Stearate 1.0 7 Pro-type glycerin monostearate 0.5 8 stearic acid 1.5 9 Glyceryl Stearate/PEG-400 Stearate 1.0 10 Propylene Glycol 3.0 11 carboxypolymer 0.1 12 triethanolamine 0.2 13 antiseptic a very small amount 14 pigment a very small amount 15 Spices a very small amount 16 Purified water a very small amount

<Manufacturing method> 10, 11, 12, 13, 16 are heated between 80 ~ 85 ℃ while mixing and stirring, and put into the manufacturing unit, and then the emulsifier works and 2, 3, 4, 5, 6, 7, 8, 9, 12 is heated and dissolved between 80 and 85℃ and then emulsified. After emulsification is finished, cool to 50℃ while stirring using a stirrer, add No. 15, cool to 45℃, add No. 14, add No. 1 to 35℃, cool to 25℃, and age.

< formulation example 3 > DKMEX (donkey milk, honey grass, green tea, quince) and Phellinus linteus mixed extract) Preparation of a nutritious cream containing the mixed extract extracted by mixing as an active ingredient

Example 7 The prescription examples of nutrient cream among cosmetics containing DKMEX (mixed extract of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus) are shown in Table 8.

number Raw material Content (% by weight) One Example 7 DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Sanghwang mushroom) 5.0 2 stearic acid 2.0 3 cetyl alcohol 2.0 4 Glyceryl Monostearate 2.0 5 Polyoxyethylene sorbitan monostearate 0.5 6 Sorbitan Sesquioleate 0.5 7 Glyceryl Monostearate/Glyceryl Stearate/Polyoxyethylene Stearate 1.0 8 wax 1.0 9 liquid paraffin 4.0 10 squalane 4.0 11 Caprylic/Caprictriglycerides 4.0 12 carboxyvinyl polymer 0.3 13 Butylene Glycol 5.0 14 glycerin 3.0 15 triethanolamine 0.5 16 Purified water balance

<Manufacturing method> 12, 13, 14, 16 are heated between 80 ~ 85 ℃ while mixing and stirring, and put into the manufacturing unit, and then the emulsifier works and 2, 3, 4, 5, 6, 7, 8, 9, 10, After dissolving by heating 11 to 80 ~ 85 ℃, put 15 into the production unit by stirring and emulsifying. After emulsification is finished, cool to 35℃ while stirring using a stirrer, add No. 1, cool to 25℃, and age.

< formulation example 4 > DKMEX (donkey milk, honey grass, green tea, quince) and mixed extract of Phellinus linteus) as an active ingredient Preparation of Essence

Example 7 The prescription examples of essences among cosmetics containing mixed extracts extracted by mixing DKMEX (mixed extracts of donkey oil, Lamiaceae, green tea, quince and Phellinus linteus) are shown in Table 9.

number Raw material Content (% by weight) One Example 7 DKMEX (mixed extract of donkey milk, Lamiaceae, green tea, quince and Sanghwang mushroom) 5.0 2 sitosterol 1.7 3 Polyglyceryl 2-oleate 1.5 4 ceramide 0.7 5 steareth-4 1.2 6 cholesterol 1.5 7 dicetyl phosphate 0.4 8 concentrated glycerin 5.0 9 macadamia oil 15.0 10 carboxyvinyl polymer 0.2 11 xanthan gum 0.2 12 antiseptic a very small amount 13 Spices a very small amount 14 Purified water balance

<Production method> 2, 3, 4, 5 and 6 are homogenized at a constant temperature and are called nonionic amphiphilic lipids. Mix the nonionic amphiphilic lipids with 7, 8 and 14, mix by adding No. 1, homogenize at a constant temperature and pass through a microfluidizer, then slowly add 9 at a constant temperature to homogenize, and then again micro Pass it through the fluidizer again. Then, 10, 11, 12, and 13 are added and dispersed to stabilize and mature.

Although the present invention has been described with the accompanying drawings, this is only one example of various embodiments including the gist of the present invention, and is intended to be easily practiced by those skilled in the art. As an object, it is clear that the present invention is not limited to the embodiments described above. Therefore, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent range by change, substitution, substitution, etc. within the scope of the present invention do not depart from the scope of the present invention. will be included In addition, it is clear that some configurations in the drawings are provided exaggerated or reduced than the actual configuration to more clearly explain the configuration.

Claims (4)

DKMEX (donkey milk, honey grass, green tea, quince, and Phellinus linteus) were mixed in a weight ratio of 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0 : 0.1 ~ 20.0, and using an extraction solvent at 60 ~ 90 ℃ conditions. A cosmetic composition having anti-oxidation, anti-inflammatory and whitening effects, comprising a mixed extract extracted under reflux, filtered, concentrated under reduced pressure and freeze-dried) as an active ingredient.
delete According to claim 1,
The extract is a mixed extract, characterized in that extracted using any one or a mixture of two or more extraction solvents selected from the group consisting of water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate, glycerin and ethylene glycol A cosmetic composition having antioxidant, anti-inflammatory and whitening effects.
According to claim 1,
The cosmetic composition is a lotion, toner, lotion, cream, foundation, essence, gel, pack, emulsified sunscreen cream, emulsified foundation, emulsified makeup base, oil cake foundation, two-way cake, or powder pact formulation A cosmetic composition having antioxidant, anti-inflammatory and whitening effects containing a mixed extract, characterized in that the formulation.