KR102552182B1 - Centrifugation apparatus and method of extracting platelet rich plasma using the same - Google Patents

Abstract

One embodiment of the present invention provides a centrifugal separator capable of rapidly and accurately extracting platelet-rich plasma with a minimized process and a method for extracting platelet-rich plasma using the same. Here, the centrifugal separator includes a tube part, a rotating part, a first needle part, a second needle part, a suction part, and a control part. The tube unit has a first diaphragm sealing the lower end of the tube body in which blood is accommodated, and a second diaphragm sealing the side surface of the tube body. A tube unit is inserted into the rotation unit, and rotated so that blood in the tube unit is separated into red blood cells, platelet rich plasma, and platelet poor plasma from the lower side to the upper side. The first needle unit has a first needle provided on the lower side of the tube body and a first driving mechanism for moving the first needle through the first diaphragm. The second needle unit has a second needle provided on the outside of the tube body and a second driving mechanism for moving the second needle through the second diaphragm. The suction unit selectively provides suction force to the first needle and the second needle. The control unit controls the first actuator to aspirate red blood cells through the first needle by controlling the suction unit when the first needle penetrates the first diaphragm and is inserted into the tube body, and then controls the second actuator to move the second needle into the tube body. When inserted into the tube body through the diaphragm, the suction part is controlled so that the platelet-poor plasma is sucked through the second needle so that only the platelet-rich plasma remains in the tube body.

Description

Centrifugal separator and platelet-rich plasma extraction method using the same

The present invention relates to a centrifugal separator and a method for extracting platelet-rich plasma using the same, and more particularly, to a centrifugal separator capable of rapidly and accurately extracting platelet-rich plasma with a minimized process and a method for extracting platelet-rich plasma using the same.

Blood carries oxygen from the lungs to tissue cells, transports carbon dioxide from tissues to the lungs for release, and carries nutrients absorbed from the digestive tract to organs or tissue cells. In addition, blood, as a tissue decomposition product, transports substances unnecessary to the body to the kidneys to be discharged out of the body, and transports hormones secreted from endocrine glands to working organs and tissues. In addition, blood maintains a constant body temperature by distributing body heat evenly, and performs various functions such as destroying and detoxifying bacteria and foreign substances invading a living body.

Although such blood is used as a major indicator for determining various diseases or health conditions, platelets rich in growth factors in the blood are used for therapeutic purposes.

Blood consists of red blood cells, white blood cells, platelets, and plasma. Of these, platelets are mainly present in plasma, and plasma is divided into platelet rich plasma (PRP) and platelet poor plasma (PPP). Among them, PRP is used for therapeutic purposes because it is implanted in the pain part, especially the knee joint, ligament, muscle, etc., and stimulates stem cells to help cell generation.

PRP is a small amount of about 1% of collected blood, and it is difficult to separate from red blood cells due to its high viscosity. In general, PRP is extracted by injecting blood collected from the human body into a PRP separation container and then using a centrifuge. In the past, a test tube was mainly used as a PRP separation vessel, but PRP extraction is cumbersome, so a separation vessel in which the liquid chamber is divided into two has recently been developed and used.

In a conventional PRP separator, blood collected from a human body is injected into the PRP separator using a syringe, and then the blood-injected PRP separator is centrifuged using a centrifuge. And, when centrifugation is completed, red blood cells, PRP, and PPP are sequentially separated into three stages from the bottom by the difference in specific gravity. At this time, PRP includes a buffy coat, which is a boundary layer divided into white thin layers formed of platelets and leukocytes.

In order to extract PRP from the structure separated into three stages, the red blood cells are raised to the part where the axillary chamber is bisected by using a lifting member provided to move from the inside of the axillary chamber, then a syringe is coupled to the upper side, and a syringe is first used. Since PPP is extracted and PRP is extracted using a different syringe, there is a problem in that the extraction process takes a lot of time.

Therefore, a technique for rapidly and accurately separating PRP with minimal processes is required.

Republic of Korea Patent Registration No. 1128163 (Announced on March 23, 2012)

In order to solve the above problems, a technical problem to be achieved by the present invention is to provide a centrifugal separator capable of rapidly and accurately extracting platelet-rich plasma with a minimized process and a method for extracting platelet-rich plasma using the same.

The technical problem to be achieved by the present invention is not limited to the above-mentioned technical problem, and other technical problems not mentioned can be clearly understood by those skilled in the art from the description below. There will be.

In order to achieve the above technical problem, an embodiment of the present invention is a tube body for receiving blood therein, a first diaphragm for sealing a lower through hole formed through the lower end of the tube body, and a side surface of the tube body. A tube portion having a second diaphragm for sealing the side through-hole formed through; It has a mounting groove formed to insert the tube part, and rotates around a rotation axis coupled to a first motor so that blood in the tube part flows from the lower side to the upper side. Red blood cells, platelet rich plasma, and platelet-poor plasma a rotating part that allows separation into poor plasma); a first needle part having a first needle provided on a lower side of the tube body and a first driving mechanism through which the first needle moves through the first diaphragm; a second needle part having a second needle provided outside the tube body and a second driving mechanism through which the second needle moves through the second diaphragm; a suction unit configured to selectively provide a suction force to the first needle and the second needle; Then, when the first needle penetrates the first diaphragm and is inserted into the tube body by controlling the first actuator, the suction unit is controlled to suck the red blood cells through the first needle, and then the second actuator a control unit that controls the suction unit so that the platelet-poor plasma is suctioned through the second needle so that only the platelet-rich plasma remains in the tube body when the second needle penetrates the second septum and is inserted into the tube body; It provides a centrifugal separator characterized in that it comprises.

In an embodiment of the present invention, a color sensing unit for sensing the presence or absence of the red blood cells by taking a picture of the lower end of the tube body is included, and the control unit detects that the red blood cells are present in the tube body, and the control unit detects that the red blood cells are present in the tube body. The part may be controlled to suck the red blood cells through the first needle, and if the color sensing part senses that there are no red blood cells in the tube body, the suction part may be controlled to suck the platelet-deficient plasma through the second needle. there is.

In an embodiment of the present invention, the first needle is provided in the direction of the central axis of the tube body at the lower side of the tube body, and the first driving mechanism is provided in a direction parallel to the first needle at the lower side of the tube body. A first screw bar provided, a first needle mount screwed to the first screw bar and fixed to the first needle, and rotating the first screw bar to reciprocate the first needle mount so that the first needle is A second motor may be inserted into the tube body through the first diaphragm or moved out of the tube body.

In an embodiment of the present invention, the second needle is provided in the direction of the central axis of the side through hole from the outside of the tube body, and the second drive mechanism is provided in a direction parallel to the second needle from the outside of the tube body. A second screw bar provided, a second needle mount screwed to the second screw bar and fixed to the second needle, and rotating the second screw bar to reciprocate the second needle mount so that the second needle is It may have a third motor that penetrates the second diaphragm and is inserted into the tube body or moved out of the tube body.

In an embodiment of the present invention, a position sensing unit for sensing the position of the rotation unit is included, and the control unit determines that the tube body is the same as the first needle based on position information of the rotation unit sensed by the position sensing unit. It is possible to stop and control the rotating part so as to be located on the central axis.

In an embodiment of the present invention, the suction unit connects a pump generating a suction force, the pump and the first needle, and a first tube through which the sucked red blood cells are moved by providing the suction force to the first needle. , It may have a second tube connecting the pump and the second needle and moving the platelet-deficient plasma by allowing the suction force to be provided to the second needle.

In an embodiment of the present invention, after the platelet-rich plasma recovery of the tube body is completed, the washing liquid is injected into the tube body, and the control unit controls the suction unit so that the washing liquid is applied to the first needle and the second needle at the same time. The tube body, the first needle, the first pipe, the second needle, and the second pipe may be cleaned by suction.

On the other hand, in order to achieve the above technical problem, one embodiment of the present invention is a platelet-rich plasma extraction method using a centrifugal separator, injecting blood into the tube body through a third diaphragm provided at the upper end of the tube body injection step; a first centrifugal separation step of inserting the tube part into the mounting groove and rotating the rotating part so that the blood is separated into blood plasma mixed with red blood cells and platelets from the lower side to the upper side; The rotation of the rotating part is stopped, the first needle penetrates the first diaphragm and is inserted into the tube body, and the red blood cells are suctioned and removed through the first needle by the suction force generated by the suction unit. removal step; a second centrifugation step of rotating the rotation unit to separate the platelet-mixed plasma from lower side to upper side into platelet-rich plasma and platelet-poor plasma; and stopping the rotation of the rotation unit, allowing the second needle to penetrate the second diaphragm and being inserted into the tube body, and suctioning and removing the platelet-deficient plasma through the second needle by a suction force generated by the suction unit. A method for extracting platelet-rich plasma using a centrifugal separator is provided, comprising a step of removing platelet-poor plasma so that only the platelet-rich plasma remains in the tube body.

In an embodiment of the present invention, in the red blood cell removing step, the controller controls the rotation unit to position the tube body on the same central axis as the first needle based on positional information of the rotation unit sensed by the position sensing unit. can be stopped and controlled.

In an embodiment of the present invention, in the red blood cell removal step, the control unit controls the suction unit to suck the red blood cells through the first needle only while the color sensing unit senses that the red blood cells are present in the tube body; , When the color sensing unit senses that the red blood cells are not present in the tube body, the operation of the suction unit may be stopped.

In an embodiment of the present invention, in the first centrifugation step, the rotating unit may be rotated at 1000 to 1500 rpm for 9 to 11 minutes.

In an embodiment of the present invention, the second centrifugation step may be performed for 9 to 11 minutes at 2500 to 3500 rpm.

In an embodiment of the present invention, the suction unit connects a pump generating a suction force, the pump and the first needle, and a first tube through which the sucked red blood cells are moved by providing the suction force to the first needle. , having a second tube connecting the pump and the second needle, through which the suctioned platelet-poor plasma is moved by providing the suction force to the second needle, and after the platelet-poor plasma removal step, the platelet-rich plasma After the extraction of the washing liquid is injected into the tube body, a washing liquid injection step, the first needle is inserted through the first diaphragm, and the second needle is inserted through the second diaphragm, and a needle insertion step , The washing liquid is sucked into the first needle and the second needle by the suction force provided by the suction part, and the tube body, the first needle, the first pipe, the second needle, and the second pipe are washed. It may include a washing step having a washing liquid suction step so as to be.

According to an embodiment of the present invention, it is possible to automatically separate red blood cells and platelet-deficient plasma separated by the centrifugation process using the first needle and the second needle so that only platelet-rich plasma remains in the tube part. The process may enable rapid and accurate extraction of platelet-rich plasma.

The effects of the present invention are not limited to the above effects, and should be understood to include all effects that can be inferred from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is a plan view showing a centrifugal separator according to an embodiment of the present invention.
2 is a front view showing a centrifugal separator according to an embodiment of the present invention.
3 is an exemplary view cut along line A-A' of FIG. 1;
4 is a cross-sectional view showing a tube part of a centrifugal separator according to an embodiment of the present invention.
5 is a cross-sectional view showing a rotating part of a centrifugal separator according to an embodiment of the present invention.
6 is an exemplary plan view showing a first needle part of a centrifugal separator according to an embodiment of the present invention.
7 is an exemplary right side view showing a first needle part of a centrifugal separator according to an embodiment of the present invention.
8 is a plan view illustrating a second needle part of a centrifugal separator according to an embodiment of the present invention.
9 is an exemplary right side view showing a second needle part of a centrifugal separator according to an embodiment of the present invention.
10 is an exemplary view showing a suction part of a centrifugal separator according to an embodiment of the present invention as a center.
11 is a configuration diagram of a suction unit of a centrifugal separator according to an embodiment of the present invention.
12 is a flowchart illustrating a method for extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention.
13 is an exemplary diagram for explaining the blood state of the tube part according to the process of extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention.
14 is an exemplary diagram for explaining the operation of the color sensing unit and the first needle during the process of extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention.
15 is a flow chart showing a washing step in the method of extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in many different forms and, therefore, is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a part is said to be "connected (connected, contacted, combined)" with another part, this is not only "directly connected", but also "indirectly connected" with another member in between. "Including cases where In addition, when a part "includes" a certain component, it means that it may further include other components without excluding other components unless otherwise stated.

Terms used in this specification are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as "include" or "have" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof described in the specification, but one or more other features It should be understood that the presence or addition of numbers, steps, operations, components, parts, or combinations thereof is not precluded.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is a plan view showing a centrifugal separation device according to an embodiment of the present invention, FIG. 2 is a front view showing a centrifugal separation device according to an embodiment of the present invention, and FIG. 3 is a line A-A' of FIG. FIG. 4 is a cross-sectional view showing a tube part of a centrifugal separator according to an embodiment of the present invention.

1 to 4, the centrifugal separator includes a tube part 100, a rotating part 200, a first needle part 300, a second needle part 400, a suction part 500, and a control part 600. ) may be included.

First, as shown in FIG. 4 , the tube unit 100 may have a tube body 110 , a first diaphragm 120 and a second diaphragm 130 .

The tube body 110 may form the body of the tube unit 100, and blood may be accommodated inside the tube body 110. A lower through-hole 111 may be formed at the lower end of the tube body 110, an upper end may be formed open, and a side through-hole 112 may be formed through the side of the tube body 110.

The first diaphragm 120 may be provided in the lower through hole 111 of the tube body 110 to seal the lower through hole 111 . The first diaphragm 120 may be formed of a Teflon-coated rubber material.

The second diaphragm 130 may be provided in the side through hole 112 to seal the side through hole 112 . Referring to FIG. 13, the side through hole 112 is preferably formed higher than the height of the Platelet Rich Plasma (PRP) 13 after the red blood cells 11 are removed. When the needle penetrates the second septum 130 and is inserted into the tube body 110, platelet poor plasma (PPP) 14 may be aspirated.

Also, the tube unit 100 may have a cap 140 and a third diaphragm 150 .

The cap 140 may be provided on the open upper end of the tube body 110 to cover the open upper end of the tube body 110 .

The third diaphragm 150 may be provided in the through hole 141 formed through the center of the cap 140 to seal the through hole 141 . The second diaphragm 130 and the third diaphragm 150 may be formed of the same material as the first diaphragm 120 .

Referring back to FIGS. 1 to 4 , the centrifugal separator may include a case part 900, a tube part 100, a rotating part 200, a first needle part 300, and a second needle part 400. ), the suction part 500 and the control part 600 may be provided inside the case part 900.

The case unit 900 may have an upper case 901 , and an opening 902 may be formed in the center of the upper case 901 . An upper part of the rotating part 200 may be exposed upward through the opening 902, the outer tube part 100 may be coupled to the rotating part 200 through the opening 902, and the rotating part 200 The tube unit 100 coupled to may fall outward through the opening 902 .

And, the case part 900 may have a side case 903 . An input unit 910 , a power switch 920 , and a power display unit 930 may be provided in a front side case of the side case 903 .

Also, the case part 900 may have an inner case 904 . The inner case 904 may be formed below the opening 902 to form an upper space 905 in which the rotation unit 200 is provided.

The input unit 910 may include a touch screen, and a control command of the centrifugal separator may be input through the touch screen. The power switch 920 may include a switch capable of turning on/off the power of the centrifugal separator. Also, the power display unit 930 may include an LED, and power on/off may be displayed by turning on the LED.

5 is a cross-sectional view showing a rotating part of a centrifugal separator according to an embodiment of the present invention.

As shown in FIGS. 3 and 5, the rotation unit 200 may be coupled to a rotation shaft 230 that is rotated by being coupled to the first motor 220 provided in the inner space 906 of the case unit 900, It can be rotated together with the rotation shaft 230. The rotation unit 200 may be rotated around the rotation shaft 230, and the first motor 220 may be a step motor.

Also, the rotating part 200 may have a mounting groove 210 . The mounting groove 210 may be formed in a shape corresponding to the tube part 100, and the tube part 100 may be inserted into the mounting groove 210. A plurality of mounting grooves 210 may be formed along the circumferential direction of the rotating part 200 .

Also, the rotating part 200 may have a first through hole 211 , a second through hole 212 , and a third through hole 213 connected to the mounting groove 210 .

The first through hole 211 may be formed in the direction of the central axis CA of the mounting groove 210 . A first needle 310 to be described later may be inserted into the first through hole 211 .

The second through hole 212 may be formed in a radial direction of the mounting groove 210 , and a second needle 410 to be described later may be inserted into the second through hole 212 . The second through hole 212 may be formed at a position corresponding to the second diaphragm 130 when the tube unit 100 is inserted into the mounting groove 210 .

The third through hole 213 may be formed in the radial direction of the mounting groove 210 , and the color sensing unit 700 to be described later may sense the tube unit 100 through the third through hole 213 .

6 is an exemplary plan view showing a first needle part of a centrifugal separator according to an embodiment of the present invention, and FIG. 7 is an exemplary right side view showing a first needle part of a centrifugal separator according to an embodiment of the present invention.

3, 5 to 7, the inner case 904 may have an inner inclined case 907, an inner horizontal case 908 and an inner round case 909. The inner inclined case 907 is formed to correspond to the side inclined part of the rotating part 200 and may be disposed to be spaced apart from the side inclined part of the rotating part 200, and the inner horizontal case 908 is formed on the lower surface of the rotating part 200. It is formed to correspond and may be disposed to be spaced apart from the lower surface of the rotating part 200 . The inner round case 909 connects the inner inclined case 907 and the inner horizontal case 908 and is formed to correspond to the lower outer round part of the rotating part 200 and is spaced apart from the lower outer round part of the rotating part 200. It can be.

The first needle part 300 may have a first needle 310 and a first driving part 320 . The first needle part 300 may be provided in the inner space 906 of the case part 900 .

The first needle 310 is provided on the central axis CA of the tube body 110 at the lower side of the tube body 110 and may be provided to extend in the direction of the central axis CA. The first needle 310 may be provided to pass through a first opening 941 formed through the inner round case 909 .

The first actuator 320 may allow the first needle 310 to reciprocate in the direction of the central axis CA of the tube body 110 . The first actuator 320 may have a first screw bar 321 , a first needle mount 322 and a second motor 323 .

The first screw bar 321 may be provided in a direction parallel to the first needle 310 at the lower side of the tube body 110 .

The first needle mount 322 may be screwed to the first screw bar 321 , and the first needle 310 may be fixed to the first needle mount 322 .

The second motor 323 may rotate the first screw bar 321 . According to the rotational direction of the first screw bar 321 , the first needle mount 322 may reciprocate along the longitudinal direction of the first screw bar 321 . When the first needle mount 322 moves upward, the first needle 310 may be introduced into the first through hole 211 through the first opening 941 and is opposed to the first through hole 211. It may be inserted into the tube body 110 through the first diaphragm 120 disposed thereon. Also, when the first needle mount 322 moves downward, the first needle 310 may move away from the tube body 110 . The second motor 323 may be a step motor.

The first needle part 300 may have a pair of first mounts 330a and 330b and a first guide bar 340 .

The first mounts 330a and 330b may be provided at both ends of the first screw bar 321, respectively, and the first screw bar 321 may be provided through the first mounts 330a and 330b. A first guide hole 331 may be formed in the mount 330b provided on the upper side of the first mounts 330a and 330b, and the first needle 310 may pass through the first guide hole 331. can Through this, one end of the first needle 310 is supported by the first needle mount 322 and the other end is supported by the first guide hole 331 to stably reciprocate linearly.

The first mounts 330a and 330b may be coupled to a first fixed mount 333 fixed to a fixed rack 332 provided in the inner space 906 of the case 900, and through this, the first needle The installation angle and installation position of the unit 300 can be firmly fixed.

The first guide bar 340 is provided in parallel with the first screw bar 321 and both ends thereof may be fixed to the first mounts 330a and 330b, respectively. Also, the first needle mount 322 may be coupled to the first guide bar 340, and through this, it may stably move in a straight line. To allow the first needle mount 322 to move more stably in a straight line, the first guide bar 340 may be provided as a pair, and both ends of the first needle mount 322 are attached to the first guide bar 340. can be combined

8 is an exemplary plan view showing a second needle part of a centrifugal separator according to an embodiment of the present invention, and FIG. 9 is an exemplary right side view showing a second needle part of a centrifugal separator according to an embodiment of the present invention.

As shown in FIGS. 8 and 9 together with FIGS. 3 and 5 , the second needle unit 400 may have a second needle 410 and a second actuator 420 . The second needle unit 400 may be provided in the inner space 906 of the case unit 900 .

The second needle 410 may be provided to extend from the outside of the tube body 110 toward the central axis of the side through hole 112 of the tube body 110 . The second needle 410 may be provided to pass through the second opening 942 formed through the inner round case 909 .

The second actuator 420 may allow the second needle 410 to reciprocate through the second diaphragm 130 provided in the side through hole 112 of the tube body 110 . The second actuator 420 may have a second screw bar 421 , a second needle mount 422 and a third motor 423 .

The second screw bar 421 may be provided in a direction parallel to the second needle 410 on the outside of the tube body 110 .

The second needle mount 422 may be screwed to the second screw bar 421 , and the second needle 410 may be fixed to the second needle mount 422 .

The third motor 423 may rotate the second screw bar 421 . According to the rotational direction of the second screw bar 421 , the second needle mount 422 may reciprocate along the longitudinal direction of the second screw bar 421 . When the second needle mount 422 is moved downward, the second needle 410 may flow into the second through hole 212 through the second opening 942 and face the second through hole 212. It can be inserted into the tube body 110 through the second diaphragm 130 disposed thereon. And, when the second needle mount 422 moves upward, the second needle 410 can move away from the tube body 110 . The third motor 423 may be a step motor.

The second needle unit 400 may have a pair of second mounts 430a and 430b and a second guide bar 440 .

The second mounts 430a and 430b may be provided at both ends of the second screw bar 421, respectively, and the second screw bar 421 may be provided through the second mounts 430a and 430b. A second guide hole 431 may be formed in the lower mount 430b of the second mounts 430a and 430b, and the second needle 410 may pass through the second guide hole 431. can Through this, one end of the second needle 410 is supported by the second needle mount 422 and the other end is supported by the second guide hole 431 so that the second needle 410 can stably reciprocate linearly.

The second mounts 430a and 430b may be coupled to the second fixed mount 432 fixed to the inner inclined case 907, and through this, the second needle part 400 is firmly installed at an angle and an installation position. can be fixed

The second guide bar 440 is provided parallel to the second screw bar 421 and both ends thereof may be fixed to the second mounts 430a and 430b, respectively. In addition, the second needle mount 422 may be coupled to the second guide bar 440, and through this, it may stably move in a straight line. In order for the second needle mount 422 to move more stably in a straight line, the second guide bar 440 may be provided as a pair, and both ends of the second needle mount 422 are attached to the second guide bar 440. can be combined

10 is an exemplary diagram showing the suction part of the centrifugal separator according to an embodiment of the present invention, and FIG. 11 is a configuration diagram of the suction part of the centrifugal separator according to an embodiment of the present invention.

As shown in FIGS. 10 and 11 together with FIG. 3 , the suction unit 500 may selectively provide suction force to the first needle 310 and the second needle 410 .

The suction unit 500 may have a pump 510 , a first tube 520 and a second tube 530 .

Pump 510 may generate a suction force. As the pump 510, a peristaltic pump capable of delivering a constant amount of liquid per unit time may be used.

The first pipe 520 may connect the pump 510 and the first needle 310 . A suction force of the pump 510 may be provided to the first needle 310 through the first pipe 520 .

The second pipe 530 may connect the pump 510 and the second needle 410, and the suction force of the pump 510 may be provided to the second needle 410 through the second pipe 530. there is.

In addition, the suction unit 500 may have a first valve 540 , a second valve 550 and a manifold 560 .

The first valve 540 is connected to the first pipe 520 to open and close the first pipe 520, and the second valve 550 is connected to the second pipe 530 to open and close the second pipe 530. can be opened. Solenoid valves may be used as the first valve 540 and the second valve 550 .

The manifold 560 may be provided between the pump 510, the first valve 540, and the second valve 550, and the manifold 560 includes the first pipe 520 and the second pipe 530. ) can be connected. Also, the manifold 560 may be connected to the pump 510 through a connection pipe 571 . Red blood cells can be aspirated and moved through the first tube 520, platelet-deficient plasma can be aspirated and moved through the second tube 530, and red blood cells and platelet-deficient plasma pass through the manifold 560. After being sucked into the pump 510, it may be discharged through the discharge pipe 572 connected to the pump 510.

As shown in FIG. 11, the pump 510 and the manifold 560 may be configured as one, and the first needle part 300 and the second needle part 400 correspond to each tube part 100. They may be provided in pairs. The first and second needles 310a and 410a, the first valve 540a and the second valve 550a, respectively, are disposed in pairs with any one tube part. The connected first pipe 520a and the second pipe 530a may be connected to the manifold 560 . And, the first needle part and the second needle part disposed in pairs with the other tube part, the first needle part 310b and the second needle part 410b, the first valve 540b and the second valve 550b The first pipe 520b and the second pipe 530b respectively connected to may also be connected to the manifold 560. And the first and second needles 310c and 410c, the first valve 540c and the second valve 550c of the first needle part and the second needle part paired with another tube part. The first pipe 520c and the second pipe 530c respectively connected to may also be connected to the manifold 560. Accordingly, it may be possible to selectively aspirate red blood cells and platelet-deficient plasma from each tube unit using a single pump 510 and a manifold 560 .

Referring back to FIGS. 3, 5 and 10 , the control unit 600 controls the first actuator 320 so that the first needle 310 penetrates the first diaphragm 120 and is inserted into the tube body 110. The red blood cells of the tube body 110 may be sucked through the first needle 310 by controlling the suction part 500 . In addition, the controller 600 may control the first actuator 320 to remove the first needle 310 from the tube body 110 when the red blood cells are sucked. Then, the controller 600 may control the second actuator 420 so that the second needle 410 penetrates the second diaphragm 130 and is inserted into the tube body 110, and the suction part 500 By controlling, the platelet-deficient plasma of the tube body 110 may be sucked through the second needle 410 . In addition, the controller 600 may control the second actuator 420 to remove the second needle 410 from the tube body 110 when the aspiration of the platelet-poor plasma is completed, and through this, the tube body 110 ) can be left with only platelet-rich plasma.

Meanwhile, the centrifugal separator may include a color sensing unit 700 .

The color sensing unit 700 may be provided in the inner space 906 of the case unit 900 and may be fixed to the inner inclined case 907 . The color sensing unit 700 may sense the presence or absence of red blood cells by photographing the lower end of the tube body 110 in a state where the tube unit 100 is inserted into the mounting groove 210 .

The controller 600 may control the suction unit 500 to suck the red blood cells through the first needle 310 when the color sensing unit 700 senses that there are red blood cells in the tube body 110 . Then, when the color sensing unit 700 senses that there are no red blood cells in the tube body 110, the controller 600 may control the suction unit 500 to stop suction through the first needle 310. Also, the controller 600 may control the suction unit 500 to suck the platelet-deficient plasma of the tube body 110 through the second needle 410 .

Also, the centrifugal separator may include a position sensing unit 800 .

The position sensing unit 800 may be provided between the internal horizontal case 908 and the rotating unit 200, and may sense the position of the rotating unit 200.

The control unit 600 controls the rotation unit 200 so that the tube body 110 is located on the same central axis as the first needle 310 based on the position information of the rotation unit 200 sensed by the position sensing unit 800. You can control the pause. Through this, the first needle 310 can stably penetrate the first diaphragm 120 and be inserted into the tube body 110, and the second needle 410 stably penetrates the second diaphragm 130 and It can be inserted into the tube body 110, and the color sensing unit 700 can stably sense the red blood cells of the tube body 110.

The control unit 600 includes a first motor 220, a second motor 323, a third motor 423, a color sensing unit 700, a position sensing unit 800, a pump 510, and a first valve 540. ) and the second valve 550 and the wire 610 may be electrically connected.

Meanwhile, after the platelet recovery of the tube body 110 is completed, a cleaning solution may be injected into the tube body 110 .

In addition, the control unit 600 may control the suction unit 500 so that the washing liquid is simultaneously sucked into the first needle 310 and the second needle 410, and the first tube 520 and the second tube 530 ) and can be discharged through the discharge pipe 572. Through this, including the tube body 110, the first needle 310, the first pipe 520, the second needle 410, and the second pipe 530, from the tube body 110 to the discharge pipe 572 The inside of each component of can be cleaned. The washing liquid may be, for example, ethanol.

Hereinafter, a method for extracting platelet-rich plasma using a centrifugal separator will be described.

12 is a flow chart showing a method for extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention, and FIG. 13 is a tube according to a process for extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention. FIG. 14 is an exemplary diagram for explaining the blood state of the unit, and FIG. 14 is an exemplary diagram for explaining the operation of the color sensing unit and the first needle during the process of extracting platelet-rich plasma using a centrifugal separator according to an embodiment of the present invention.

As shown in FIGS. 12 to 14 together with FIGS. 3 and 4, the platelet-rich plasma extraction method using a centrifugal separator includes an injection step (S1110), a first centrifugation step (S1120), and a red blood cell removal step (S1130). , a second centrifugation step (S1140) and a plasma removal step (S1150).

The injection step ( S1110 ) may be a step of injecting the blood 10 into the tube body 110 through the third diaphragm 150 provided at the upper end of the tube body 110 .

In the first centrifugal separation step (S1120), the tube unit 100 is inserted into the mounting groove 210 of the rotation unit 200, and the rotation unit 200 is rotated so that the blood 10 moves from the lower side to the upper side of the red blood cells 11. and platelets may be separated into the mixed blood plasma 12 .

In the first centrifugation step (S1120), the rotating unit 200 may be rotated at 1000 to 1500 rpm for 9 to 11 minutes. Accordingly, the blood 10 can be separated into two stages of red blood cells 11 and platelet-mixed plasma 12 instead of being separated into three stages of red blood cells, platelet-rich plasma, and platelet-poor plasma. That is, in the platelet-mixed plasma 12, there may be no distinction between platelet-rich plasma and platelet-poor plasma.

The red blood cell removal step (S1130) stops the rotation of the rotating part 200, allows the first needle 310 to penetrate the first septum 120 and is inserted into the tube body 110, and causes the suction part 500 to This may be a step of removing the red blood cells 11 by being sucked through the first needle 310 by a suction force.

At this time, the position sensing unit 800 senses the position of the rotation unit 200, and the control unit 600, based on the position information of the rotation unit 200 sensed by the position sensing unit 800, the tube body 110 Stopping of the rotation unit 200 may be controlled to be positioned on the same central axis as the first needle 310 .

Also, the color sensing unit 700 may sense the presence or absence of the red blood cells 11 by photographing the lower end of the tube body 110 . If it is determined that red blood cells 11 are present based on the information sensed by the color sensor 700, the controller 600 may control the red blood cells 11 to continue to be sucked. Then, when it is determined that there are no more red blood cells 11 in the tube body 110 as the suction of the red blood cells 11 continues, the control unit 600 controls the suction unit 500 so that the suction force is not provided to the first needle 310. , and the first needle 310 can be removed from the tube body 110.

The second centrifugation step (S1140) may be a step of rotating the rotation unit 200 so that the platelet-mixed plasma 12 is separated from the bottom to the top into platelet-rich plasma 13 and platelet-poor plasma 14. there is.

The second centrifugation step (S1140) may be performed for 9 to 11 minutes at a higher rpm of 2500 to 3500 than in the first centrifugation step (S1120), through which platelet-mixed plasma (12) It can be separated into platelet-rich plasma 13 and platelet-poor plasma 14 from the lower side to the upper side.

The plasma removal step (S1150) stops the rotation of the rotating part 200, allows the second needle 410 to penetrate the second diaphragm 130 and is inserted into the tube body 110, and causes the suction part 500 to This may be a step of removing the platelet-poor plasma 14 by being sucked through the second needle 410 by a suction force, and through this, only the platelet-rich plasma 13 may remain in the tube body 110 .

The position sensing unit 800 senses the position of the rotation unit 200 rotated for secondary centrifugal separation, and the control unit 600 based on the location information of the rotation unit 200 sensed by the position sensing unit 800, Stopping of the rotating part 200 may be controlled so that the tube body 110 is positioned on the same central axis as the first needle 310 . Through this, the second needle 410 can be stably inserted into the tube body 110 by penetrating the second diaphragm 130 .

The user can take out the tube unit 100 from the rotation unit 200 and recover the platelet-rich plasma 13 accommodated in the tube unit 100 .

Meanwhile, the platelet-rich plasma extraction method using a centrifuge may include a washing step (S1160) performed after the platelet-rich plasma is extracted after the plasma removal step (S1150).

15 is a flow chart showing a washing step in the platelet-rich plasma extraction method using a centrifugal separator according to an embodiment of the present invention.

As shown further including FIG. 15 , the washing step (S1160) may have a washing solution injection step (S1161), a needle insertion step (S1162), and a washing solution suction step (S1163).

The washing liquid injection step (S1161) may be a step in which the washing liquid is injected into the tube body 110.

The needle insertion step ( S1162 ) may be a step in which the first needle 310 is inserted through the first diaphragm 120 and the second needle 410 is inserted through the second diaphragm 130 .

In the washing liquid suction step (S1163), the washing liquid is sucked into the first needle 310 and the second needle 410 by the suction force provided by the suction unit 500, and the tube body 110 and the first needle 310 , It may be a step of cleaning the first tube 520, the second needle 410 and the second tube 530. Through the washing step (S1160), the inside of each component of the centrifugal separator can be cleaned.

The above description of the present invention is for illustrative purposes, and those skilled in the art can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts should be interpreted as being included in the scope of the present invention.

10: blood 11: red blood cells
12: Plasma mixed with platelets 13: Plasma rich in platelets
14: platelet-poor plasma 100: tube part
120: first diaphragm 130: second diaphragm
200: rotating part 210: mounting groove
300: first needle part 310: first needle
400: second needle part 410: second needle
500: suction unit 520: first tube
530: second pipe 540: first valve
550: second valve 560: manifold
600: control unit 700: color sensing unit
800: position sensing unit 900: case unit
904: inner case

Claims (13)

A tube body accommodating blood therein, a first diaphragm sealing a lower through hole formed through the lower end of the tube body, and a second diaphragm sealing the side through hole formed through a side surface of the tube body. tube part;
It has a mounting groove formed to insert the tube part, and rotates around a rotation axis coupled to a first motor so that blood in the tube part flows from the lower side to the upper side. Red blood cells, platelet rich plasma, and platelet-poor plasma a rotating part that allows separation into poor plasma);
a first needle part having a first needle provided on a lower side of the tube body and a first driving mechanism through which the first needle moves through the first diaphragm;
a second needle part having a second needle provided outside the tube body and a second driving mechanism through which the second needle moves through the second diaphragm;
a suction unit configured to selectively provide a suction force to the first needle and the second needle; and
When the first needle penetrates the first diaphragm and is inserted into the tube body by controlling the first actuator, the suction unit is controlled so that the red blood cells are sucked through the first needle, and then the second actuator is controlled. and a controller configured to control the suction unit so that the platelet-poor plasma is suctioned through the second needle so that only the platelet-rich plasma remains in the tube body when the second needle penetrates the second septum and is inserted into the tube body. A centrifugal separator characterized in that for doing. According to claim 1,
A color sensing unit for sensing the presence or absence of the red blood cells by photographing the lower end of the tube body;
The control unit
When the color sensing unit senses that the red blood cells are present in the tube body, controls the suction unit to suck the red blood cells through the first needle;
The centrifugal separation device according to claim 1 , wherein the suction unit controls the suction unit to suck the platelet-deficient plasma through the second needle when the color sensing unit senses that there are no red blood cells in the tube body. According to claim 1,
The first needle is provided in the direction of the central axis of the tube body from the lower side of the tube body,
The first driving mechanism
A first screw bar provided in a direction parallel to the first needle at the lower side of the tube body;
A first needle mount screwed to the first screw bar and to which the first needle is fixed;
Having a second motor for rotating the first screw bar so that the first needle mount is reciprocally moved so that the first needle penetrates the first diaphragm and is inserted into the tube body or moved out of the tube body centrifugal separator. According to claim 1,
The second needle is provided in the direction of the central axis of the side through hole from the outside of the tube body,
The second driving mechanism
A second screw bar provided in a direction parallel to the second needle on the outside of the tube body;
A second needle mount screwed to the second screw bar and to which the second needle is fixed;
By rotating the second screw bar to reciprocate the second needle mount so that the second needle penetrates the second diaphragm and is inserted into the tube body or moved out of the tube body. Characterized in that it has a third motor centrifugal separator. According to claim 1,
Including a position sensing unit for sensing the position of the rotation unit,
The centrifugal separation device according to claim 1 , wherein the control unit stops and controls the rotation unit so that the tube body is positioned on the same central axis as the first needle, based on position information of the rotation unit sensed by the position sensing unit. According to claim 1,
the suction part
A pump that generates a suction force;
a first tube connecting the pump and the first needle and allowing the suction force to be applied to the first needle to move the sucked red blood cells;
and a second tube connecting the pump and the second needle and allowing the suction force to be provided to the second needle so that the suctioned platelet-deficient plasma is moved. According to claim 6,
After the platelet-rich plasma recovery of the tube body is completed, a cleaning solution is injected into the tube body;
The controller controls the suction unit so that the washing liquid is simultaneously sucked into the first needle and the second needle so that the tube body, the first needle, the first pipe, the second needle, and the second pipe are washed. A centrifugal separator characterized in that for doing. A method for extracting platelet-rich plasma using the centrifugal separator according to any one of claims 1 to 7,
an injection step of injecting blood into the tube body through a third diaphragm provided at an upper end of the tube body;
a first centrifugal separation step of inserting the tube part into the mounting groove and rotating the rotating part so that the blood is separated into blood plasma mixed with red blood cells and platelets from the lower side to the upper side;
The rotation of the rotating part is stopped, the first needle penetrates the first diaphragm and is inserted into the tube body, and the red blood cells are suctioned and removed through the first needle by the suction force generated by the suction unit. removal step;
a second centrifugation step of rotating the rotation unit to separate the platelet-mixed plasma from lower side to upper side into platelet-rich plasma and platelet-poor plasma; and
Stop rotation of the rotation unit, allow the second needle to penetrate the second diaphragm and be inserted into the tube body, and remove the platelet-deficient plasma by suctioning through the second needle by the suction force generated by the suction unit The method of extracting platelet-rich plasma using a centrifugal separator, characterized in that it comprises a step of removing platelet-poor plasma by doing so so that only the platelet-rich plasma remains in the tube body. According to claim 8,
In the red blood cell removal step,
The control unit stops and controls the rotation unit so that the tube body is located on the same central axis as the first needle based on position information of the rotation unit sensed by the position sensing unit. Rich Plasma Extraction Method. According to claim 8,
In the red blood cell removal step,
The control unit controls the suction unit to suck the red blood cells through the first needle only while the color sensing unit senses that the red blood cells are present in the tube body, and the color sensing unit detects that there are no red blood cells in the tube body. A method of extracting platelet-rich plasma using a centrifugal separation device, characterized in that when sensing, the operation of the suction unit is stopped. According to claim 8,
In the first centrifugation step, the platelet-rich plasma extraction method using a centrifugal separator, characterized in that the rotating part is rotated for 9 to 11 minutes at 1000 to 1500 rpm. According to claim 8,
The second centrifugation step is a platelet-rich plasma extraction method using a centrifugal separator, characterized in that carried out for 9 to 11 minutes at 2500 to 3500 rpm. According to claim 8,
the suction part
A pump generating a suction force is connected to the pump and the first needle, and a first tube through which the sucked red blood cells are moved by providing the suction force to the first needle is connected to the pump and the second needle, , having a second tube through which the suctioned platelet-deficient plasma is moved by providing the suction force to the second needle;
After the platelet-poor plasma removal step, after the platelet-rich plasma is extracted,
A washing liquid injection step in which a washing liquid is injected into the tube body;
A needle insertion step in which the first needle is inserted through the first diaphragm and the second needle is inserted through the second diaphragm;
The washing liquid is sucked into the first needle and the second needle by the suction force provided by the suction part so that the tube body, the first needle, the first pipe, the second needle, and the second pipe are washed. A method for extracting platelet-rich plasma using a centrifugal separator, comprising a washing step having a washing solution aspiration step.

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