KR102541809B1 - Antimicrobial system with antimicrobial synergistic effect - Google Patents

Abstract

It relates to an antibacterial system having an antibacterial synergistic effect, and the antibacterial system and cosmetic composition according to one aspect have a remarkable antibacterial synergistic effect against bacteria and fungi, so that even a small amount of antibacterial agent can exert an optimal effect.

Description

Antimicrobial system with antimicrobial synergistic effect}

It relates to an antibacterial system having an antibacterial synergistic effect.

Cosmetics mainly contain oil or water, and in particular, contain glycerin or sorbitol, which are carbon sources for microorganisms, and amino acid derivatives, proteins, etc., as nitrogen sources. Therefore, like foods with similar ingredients, microorganisms such as molds and bacteria are easy to penetrate and are advantageous for propagation.

Antibacterial agents are formulated into cosmetics to inhibit the proliferation of microorganisms that contaminate cosmetics from the outside, and to prevent changes in products by killing microorganisms. However, preservatives are regulated by the FDA of the United States, the Ministry of Health and Welfare of Japan, and the Korea Food and Drug Administration, and the media reports that they are harmful, increasing consumer anxiety. In spite of these problems, if antibacterial agents are not used, serious problems such as product deterioration and contamination by microorganisms or germs may occur, so the use of antimicrobial agents is essential.

However, synthetic antibacterial agents have problems such as the possibility of allergic reaction and generation of endocrine disruptors, and the induction of resistant bacteria, and the problem of excessive use of antibacterial agents while ignoring the safety of consumers has emerged.

Due to the above problems, many studies are currently being conducted to use natural antibacterial substances as preservatives to replace chemical antibacterial agents. In particular, efforts are being made to impart stable antibacterial activity against bacteria and fungi by mixing new and existing raw materials.

Korea Patent Registration 10-1772756

One aspect is to provide an antimicrobial system comprising a glyceryl ether and a polyol.

Another aspect is to provide a cosmetic composition comprising the antibacterial system.

One aspect provides an antimicrobial system comprising a glyceryl ether and a polyol.

As used herein, the term "glyceryl ether" may be one in which a hydroxy residue of glycerin forms an ether bond. The glyceryl ether may be a glyceryl monoalkyl ether, a glyceryl dialkyl ether, a glyceryl trialkyl ether, or a mixture thereof. The glyceryl ether may be substituted with a saturated or unsaturated, branched or unbranched alkyl moiety.

In one embodiment, the glyceryl ether is heptyl glycerin, ethylhexyl glycerin, caprylyl glyceryl ether, glyceryl capryl ether, chimyl alcohol, batyl alcohol, glyceryl allyl ether, glyceryl lauryl ether, It may include at least one selected from the group consisting of isodecyl glyceryl ether, isostearyl glyceryl ether, and oleyl glyceryl ether. For example, the glyceryl ether may include heptylglycerin.

According to one embodiment, the glyceryl ether is 0.1 to 75% by weight, for example, 1 to 75% by weight, 1 to 70% by weight, 1 to 50% by weight, 1 to 30% by weight, based on the total weight of the antimicrobial system 1 to 10 wt%, 5 to 75 wt%, 5 to 70 wt%, 5 to 50 wt%, 5 to 30 wt%, 5 to 10 wt%, 10 to 75 wt%, 10 to 70 wt%, 10 to 10 wt% 50 wt%, 10 to 30 wt%, 10 to 20 wt%, 30 to 75 wt%, 30 to 70 wt%, 30 to 50 wt%, 50 to 75 wt%, 50 to 70 wt%, 50 to 60 wt% %, 60 to 75% by weight, 60 to 70% by weight, or 70 to 75% by weight.

In this specification, the term “polyol” may mean an aliphatic compound having two or more hydroxyl groups (—OH).

According to one embodiment, the polyol is propanediol, butanediol, hexanediol, heptandiol, octanediol, pentylene glycol, glyceryl Caprylate, glycerol, erythritol, mannitol, sorbitol, xylitol, ribitol, ethylene glycol, propylene Propylene glycol, butylene glycol, hexylene glycol, benzyl glycol, butoxydiglycol, and diglycerin ) may include any one or more selected from the group consisting of.

For example, the polyol may include heptanediol, pentylene glycol, glyceryl caprylate, or a combination thereof.

According to one embodiment, the polyol may include at least two polyols selected from the group consisting of heptanediol, pentylene glycol, and glyceryl caprylate.

According to one embodiment, the polyol is 0.1 to 99% by weight, for example, 0.1 to 90% by weight, 1 to 90% by weight, 1 to 80% by weight, 1 to 70% by weight, 1 to 99% by weight based on the total weight of the antimicrobial system 60 wt%, 1 to 50 wt%, 1 to 40 wt%, 1 to 30 wt%, 1 to 20 wt%, 1 to 10 wt%, 10 to 90 wt%, 10 to 70 wt%, 10 to 50 wt% %, 10 to 30% by weight, 10 to 20% by weight, 30 to 90% by weight, 30 to 70% by weight, 30 to 50% by weight, 50 to 90% by weight, 50 to 70% by weight, 50 to 60% by weight, 60 to 90% by weight, 60 to 80% by weight, 60 to 70% by weight, 70 to 90% by weight, 70 to 80% by weight, 80 to 90% by weight or 85 to 90% by weight.

The antibacterial system may further include other additional antibacterial components.

According to one embodiment, the antibacterial system may have improved antibacterial activity. For example, the antimicrobial system may have improved antibacterial activity compared to using glyceryl ether or polyol alone.

According to one embodiment, the antibacterial system may have antibacterial activity against bacteria or fungi.

In this specification, the term "bacteria" may mean a representative taxon of prokaryotes that occupy one of the three domains of living organisms along with archaea and eukaryotes.

According to one embodiment, the bacteria are Escherichia coli, Pseudomonas aeruginosa , Staphylococcus aureus , Burkholderia cepacia , Pluralibacter gergoviae , Streptococcus mutans , Propionibacterium acnes , Staphylococcus epidermidis, Klebsiella pneumoniae , Enterobacter cloacae , Enterobacter gergo Biae ( Enterobacter gergoviae ), Pseudomonas mendocina ( Pseudomonas mendocina ), Pseudomonas stutzeri ( Pseudomonas stutzeri ) Pseudomonas fluorescens ( Pseudomonas fluorescens ), Pseudomonas putida ( Pseudomonas putida ), Alcaligenes faecalis , Ochrobactrum tritici, Kocuria rhizophila , Rhizobium radiobacter , Comomonas aquatic , Sphingobium yanoikuyae and Tau It may be any one or more selected from the group consisting of Lera Mechernichensis ( Thauera mechernichensis ).

In this specification, the term "fungus" may mean having the meaning of 'real fungus', and may be used interchangeably with mold. The fungi may include both yeast-like fungi and filamentous fungi. In addition, the fungus may include yeast and mold.

According to one embodiment, the fungi are Candida albicans , Candida tropicalis , Candida stellatoidea, Candida glabrata , Candida krusei , Candida guilliermondii, Candida viswanathii, Candida lusitaniae , Yarrowia lipolytica , Cryptococcus neoformans , Cryptococcus Cryptococcus gattii , Rhodotorula mucilaginosa , Saccharomyces cerevisiae, Aspergillus brasiliensis , Aspergillus flavus , Penicillium camemberti , Penicillium bilaiae, Penicillium chrysogenum, Penicillium claviforme , Penicillium crustosum , Selected from the group consisting of Penicillium roqueforti, Penicillium expansum, Penicillium puberulum , Penicillium verrucosum and Penicillium digitatum can be one or more of the

The above Candida albicans, Candida tropicalis, Candida stellatoidea, Candida glabrata, Candida krusei, Candida gulieremondi, Candida viswanati, Candida lucitanie, Yarrowia lipolytica, Cryptococcus neoformans , Cryptococcus gattii, Rhodotorula Musillazinosa, Saccharomyces cerevisiae are yeasts, Aspergillus brasiliensis, Aspergillus flavus, Penicillium camemberti, Penicillium villaiae, Penicillium Chrysogenium, Penicillium claviforme, Penicillium crustosium, Penicillium roqueforti, Penicillium expansum, Penicillium furverulum, Penicillium verucosum and Penicillium digitatum may be fungi.

Another aspect provides a cosmetic composition comprising the antibacterial system.

The antibacterial system is as described above.

The antimicrobial system is 0.0001 to 50% by weight relative to the total weight of the cosmetic composition, for example, 0.0001 to 0.001% by weight, 0.001 to 0.01% by weight, 0.01 to 0.1% by weight, 0.1 to 1% by weight, 1 to 5% by weight, 1 to 10% by weight, 10 to 20% by weight, 20 to 30% by weight, 30 to 40% by weight, or 40 to 50% by weight.

The cosmetic composition may be a solubilizing formulation or an emulsifying formulation, but is not limited thereto. In addition, the cosmetic composition may be prepared in any formulation commonly prepared in the art to which the present invention belongs. For example, it may be formulated into softening lotion, nutrient lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc. It is not limited. More specifically, skin lotion, skin softener, skin toner, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulations.

The cosmetic composition may include additional ingredients commonly used in cosmetics, such as thickeners, dispersants, fragrances, fillers, preservatives, preservatives, neutralizers, sweeteners, vitamins, free-radical scavengers, metal ion sequestering agents, functional ingredients and these It may further include any conventional cosmetic ingredient which may be selected from mixtures of One skilled in the art may select any additional ingredients and/or amounts thereof such that the beneficial properties of the compositions according to the present disclosure are not adversely affected or substantially unaffected by the anticipated addition.

When the cosmetic composition is a surfactant-containing cleansing formulation, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether Sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester may be further included.

If the cosmetic composition according to the present invention is a cream or gel formulation, it may further contain animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, zinc oxide, etc. as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters and the like may be further included.

When the cosmetic composition is a suspension formulation, as a carrier component, liquid diluents such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, suspending agents such as polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may further be included.

When the cosmetic composition is in a powder or spray formulation, it may further include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and in particular, in the case of a spray formulation, additionally chlorofluorohydrocarbon , a propellant such as propane/butane or dimethyl ether.

The cosmetic composition may be used by applying alone or overlapping, or by overlapping with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

The antibacterial system and cosmetic composition according to one aspect have a remarkable synergistic antibacterial effect against bacteria and fungi, so even a small amount of antibacterial agent can exert an optimal effect.

It will be described in more detail through the following examples. However, these examples are for illustrative purposes only and the scope of the present invention is not limited to these examples.

Example 1. Measurement of antibacterial activity of antibacterial system containing glyceryl ether or polyol

1.1 Measurement of antibacterial activity of heptylglycerin alone

In order to measure the antimicrobial activity of heptylglycerin alone, Staphylococcus aureus: S. aureus , ATCC 6538), Pseudomonas aeruginosa: P. aeruginosa , ATCC 9027), Escherichia coli ( Escherichia coli: E. coli , ATCC 8739) , Candida albicans: C. albicans , ATCC 10231), Aspergillus brasiliensis: A. brasiliensis , ATCC 16404), Burkholderia cepacia complex: B. cepacia , Pluralibacter gergoviae ( P. gergoviae ), Streptococcus mutans ( Streptococcus mutans: S. mutans ), and Propionibacterium acnes ( Propionibacterium acnes: P. acnes ) Antimicrobial activity against It was measured through a minimum inhibitory concentration (MIC) test.

First, it was prepared by dissolving heptylglycerin in a strain compatible medium according to concentration: the strain compatible medium was TSB for the bacteria S. aureus, P. aeruginosa, E. coli, B. cepacia, and P. gergoviae , and for S. mutans BHI broth for P. acnes, RCM broth for P. acnes ; SDB for the fungi C. albicans and A. brasiliensis . Then, each bacteria was inoculated into the medium at a concentration of 1 X 10 ⁵ to 1 X 10 ⁶ CFU/mL. And 32.5 ℃ for S. aureus, P. aeruginosa, E. coli, B. cepacia, P. gergoviae and yeast ( C. albicans ), 22.5 ℃ for mold ( A. brasiliensis ), S. mutans and P. acnes In the case of was incubated at a temperature of 37 ℃. Changes in each strain were measured after 1 to 2 days for bacteria and 2 to 3 days for fungi, and the results are shown in Table 1.

strain Heptylglycerine MIC (%) S. aureus 0.25 P. aeruginosa 0.5 E. coli 0.125 C. albicans 0.5 A. brasiliensis 0.125 B. cepacian 0.25 P. gergoviae 0.25 S. mutans 0.25 P. acnes 0.25

As shown in Table 1, it was confirmed that the strain died at a concentration of 0.1 to 0.5% of heptylglycerin.

1.2 Measurement of antibacterial activity of heptanediol, pentylene glycol, or glyceryl caprylate alone

The antibacterial activity of heptanediol, pentylene glycol, and glyceryl caprylate was measured in the same manner as in Example 1.2, and the results are shown in Table 2.

strain Heptanediol MIC (%) Pentylene glycol MIC (%) Glyceryl Caprylate MIC (%) S. aureus One 5 0.06 P. aeruginosa 0.5 1.25 0.5 E. coli 0.5 2.5 0.25 C. albicans One 5 0.06 A. brasiliensis 0.25 2.5 0.125 B. cepacian 0.5 1.25 0.25 P. gergoviae 0.5 2.5 >1 S. mutans 0.5 5 0.06 P. acnes 0.5 5 0.125

As shown in Table 2, at a concentration of 0.2 to 1% for heptanediol, at a concentration of 1 to 5% for pentylene glycol, and at a concentration of 0.05 or more for glyceryl caprylate, the strain was killed. Confirmed.

Example 2. Measurement of antibacterial synergistic effect of antibacterial system containing glyceryl ether and polyol

Staphylococcus aureus (S. aureus , ATCC 6538), Pseudomonas aeruginosa: P. aeruginosa , ATCC 9027), Escherichia coli ( Escherichia coli: E. coli , ATCC 8739) in an antibacterial system containing glyceryl ether and polyol ), Candida albicans: C. albicans , ATCC 10231), Aspergillus brasiliensis: A. brasiliensis , ATCC 16404), Burkholderia cepacia complex: B. cepacian , Pluralibacter gergoviae: P. gergoviae ), Streptococcus mutans: S. mutans , and Propionibacterium acnes: Antibacterial activity against Propionibacterium acnes: P. acnes The synergistic effect was measured through checkerboard assay.

Specifically, heptylglycerin; And heptanediol, pentylene glycol, glyceryl caprylate, or a combination thereof was mixed and dissolved in a strain-compatible medium such as SDB, RCM broth, BHI broth, or TSB, and prepared by concentration: bacteria E. coli, Pseudomonas aeruginosa, TSB medium was used for Staphylococcus aureus, Burkholderia cepacia, and Fluralibacter gergovia, BHI medium was used for Streptococcus mutans, and RCM medium was used for Propionibacterium acnes SDB medium was used for the fungi Candida albicans and Aspergillus brasiliensis. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Burkholderia cepacia, Fluralibacter gergoviae, Streptococcus mutans, Propionibacterium acnes, Candida albicans at a concentration of 1 X 10 ⁵ CFU/mL in the medium Kans and Aspergillus brasiliensis strains were respectively inoculated. And 32.5 ℃ for S. aureus, P. aeruginosa, E. coli, B. cepacia, P. gergoviae and yeast ( C. albicans ), 22.5 ℃ for mold ( A. brasiliensis ), S. mutans and P. acnes In the case of was incubated at a temperature of 37 ℃. Changes in each strain were measured after 1 to 2 days for bacteria and 2 to 3 days for fungi.

Fractional inhibitory concentration (FIC) was calculated according to:

FIC of Material A = (MIC of Material A in formulation)/(MIC of Material A alone);

FIC of Material B = (MIC of Material B in formulation)/(MIC of Material B alone);

FIC index = FIC of substance A + FIC of substance B.

Synergy criteria are shown in Table 3 below. The results according to this are shown in Tables 4 to 12.

FIC index Interpretation FIC index ≤ 0.5 synergistic effect 0.5 < FIC index ≤ 1 Additive effect 1 < FIC index ≤ 4 indifferent FIC index > 4 antagonistic effect

S. aureus A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.03 0.125 0.1875 synergistic effect pentylene glycol 0.125 1.25 0.5 synergistic effect Glyceryl Caprylate 0.06 0.03 0.625 side effects

P. aeruginosa A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.25 0.125 0.5 synergistic effect pentylene glycol 0.25 1.25 1.25 irrelevant Glyceryl Caprylate 0.125 0.5 0.625 side effects

E. coli A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.125 0.25 1.0 side effects pentylene glycol 0.125 1.25 1.0 side effects Glyceryl Caprylate 0.125 0.06 1.0 side effects

C. albicans A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.125 0.25 1.0 side effect pentylene glycol 0.5 2.5 1.0 side effect Glyceryl Caprylate 0.125 0.06 1.0 side effect

A. brasiliensis A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.06 0.125 1.0 side effect pentylene glycol 0.06 1.25 1.0 side effect Glyceryl Caprylate 0.06 0.06 1.0 side effect

B. cepacia A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.06 0.125 0.375 synergistic effect pentylene glycol 0.25 0.625 1.0 side effect Glyceryl Caprylate 0.125 0.06 0.5 synergistic effect

P. gergoviae A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.25 0.25 0.75 side effect pentylene glycol 0.5 1.25 One side effects Glyceryl Caprylate 0.125 One 1.25 irrelevant

P. acnes A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.125 0.06 0.375 synergistic effect pentylene glycol 0.25 2.5 One side effect Glyceryl Caprylate 0.125 0.03 0.5 synergistic effect

S. mutans A B MIC (%) for material combination FICI _m Interpretation A B heptylglycerin 1,2-heptanediol 0.25 0.125 0.75 side effect pentylene glycol 0.125 1.25 0.5 synergistic effect Glyceryl Caprylate 0.125 0.03 0.75 side effect

A: Heptylglycerin + 1,2-heptanediol
B: glyceryl caprylate strain MIC (%) for material combination FICI _m Interpretation A B S. aureus 0.01 + 0.03 0.01 0.3125 synergistic effect P. aeruginosa 0.06 + 0.12 0.25 0.5 synergistic effect E. coli 0.01 + 0.03 0.06 0.375 synergistic effect C. albicans 0.125 + 0.25 0.06 One side effect A. brasiliensis 0.03 + 0.06 0.03 0.5 synergistic effect B. cepacia 0.03 + 0.06 0.06 0.375 synergistic effect P. gergoviae 0.125 + 0.25 One 1.0 side effect P. acnes 0.03 + 0.06 0.03 0.375 synergistic effect S. mutans 0.06 + 0.125 0.01 0.5 synergistic effect

A: Heptylglycerin + 1,2-heptanediol
B: pentylene glycol strain MIC (%) for material combination FICI _m Interpretation A B P. gergoviae 0.06 + 0.125 0.125 0.75 synergistic effect

As shown in Tables 4 to 14, specifically, heptylglycerin; And an antibacterial system comprising heptanediol, pentylene glycol, glyceryl caprylate, or a combination thereof is Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Candida albicans, Aspergillus brasiliensis, Burkholderia cepha It was confirmed that it has an antibacterial synergistic effect against Sia, Fluralibacter gergoviae, Streptococcus mutans, and Propionibacterium acnes.

In particular, antibacterial systems containing heptylglycerin and two or more different polyols, for example, heptylglycerin, 1,2-heptanediol, and glyceryl caprylate or pentylene glycol, are significantly It was confirmed that it had an improved antibacterial synergistic effect.

Additionally, when heptylglycerin was not included, an antibacterial synergistic effect was confirmed, and the results are shown in Table 15.

A: 1,2-heptanediol
B: glyceryl caprylate strain MIC (%) for material combination FICI _m Interpretation A B S. aureus 0.25 0.01563 0.375 synergistic effect P. aeruginosa 0.5 0.125 0.75 side effects E. coli 0.25 0.0625 0.5 synergistic effect C. albicans 0.25 0.0625 0.5 synergistic effect A. brasiliensis 0.25 0.125 1.0 side effects B. cepacia 0.125 0.25 1.0 side effects P. gergoviae 0.25 One 1.5 irrelevant P. acnes 0.06 0.03 0.375 synergistic effect S. mutans 0.25 0.03 1.0 side effect

As shown in Tables 13 and 15, when heptylglycerin, a glyceryl ether, was not included, it was confirmed that the synergistic antibacterial effect in bacteria and fungi was reduced compared to the antibacterial system containing heptylglycerin.

The above results mean that the antibacterial activity of the antibacterial system including glyceryl ether and polyol can be significantly improved compared to the use of glyceryl ether or polyol alone.

Claims (10)

glyceryl ethers including heptylglycerin; and
An antimicrobial system comprising a polyol comprising heptandiol, pentylene glycol, glyceryl caprylate or a combination thereof. The method of claim 1,
The glyceryl ether is ethylhexyl glycerin, caprylyl glyceryl ether, glyceryl capryl ether, chimyl alcohol, batyl alcohol, glyceryl allyl ether, glyceryl lauryl ether, isodecyl glyceryl ether, isoste An antibacterial system further comprising at least one selected from the group consisting of aryl glyceryl ether and oleyl glyceryl ether. The method of claim 1,
The polyol is propanediol, butanediol, hexanediol, octanediol, glycerol, erythritol, mannitol, sorbitol, xylitol ), ribitol, ethylene glycol, propylene glycol, butylene glycol, hexylene glycol, benzyl glycol ( benzyl glycol), butoxydiglycol (butoxydiglycol), and diglycerin (diglycerin) further comprising any one or more selected from the group consisting of, antibacterial system. The method of claim 1,
The polyol is heptandiol; and
An antibacterial system comprising pentylene glycol, glyceryl caprylate or a combination thereof. The method of claim 1,
The antibacterial system is an antibacterial system that has improved antibacterial activity compared to using glyceryl ether or polyol alone. The method of claim 1,
The glyceryl ether is contained in 0.1 to 75% by weight relative to the total weight of the antimicrobial system, the antimicrobial system. The method of claim 1,
The polyol is an antimicrobial system that is included in 0.1 to 99% by weight relative to the total weight of the antimicrobial system. The method of claim 1,
An antibacterial system having antibacterial activity against bacteria or fungi. The method of claim 8,
The bacteria are Escherichia coli ( Escherichia coli ), Pseudomonas aeruginosa ( Pseudomonas aeruginosa ), Staphylococcus aureus ( Staphylococcus aureus ), Burkholderia cepacia ( Burkholderia cepacia ), Pluralibacter gergoviae ( Pluralibacter gergoviae ), Streptococcus mutans ( Streptococcus mutans ), Propionibacterium acnes ( Propionibacterium acnes ), Staphylococcus epidermis ( Staphylococcus epidermidis ), Bacillus pneumoniae ( Klebsiella pneumoniae ), Enterobacter cloacae ( Enterobacter cloacae ), Enterobacter gergoviae ), Pseudomonas mendocina, Pseudomonas stutzeri , Pseudomonas fluorescens , Pseudomonas putida , Alcaligenes faecalis , Ocrobacterum tree Tish ( Ochrobactrum tritici ), Kocuria rhizophila , Rhizobium radiobacter , Comomonas aquatic , Sphingobium yanoikuyae , and Taurera mechanikensis ( Thauera mechernichensis ) At least one selected from the group consisting of,
The fungi are Candida albicans, Candida tropicalis , Candida stellatoidea, Candida glabrata , Candida krusei , Candida gulieremondi ( Candida guilliermondii ), Candida viswanathii, Candida lusitaniae , Yarrowia lipolytica, Cryptococcus neoformans , Cryptococcus gattii , Rhodotorula mucilaginosa , Saccharomyces cerevisiae , Aspergillus brasiliensis , Aspergillus flavus , Penicillium camemberti ( Penicillium camemberti ), Penicillium bilaiae ), Penicillium chrysogenum , Penicillium claviforme ), Penicillium crustosum , Penicillium roqueforti ( Penicillium roqueforti ), Penicillium expansum, Penicillium puberulum , Penicillium verrucosum and Penicillium digitatum At least one selected from the group consisting of, antibacterial system. A cosmetic composition comprising the antibacterial system of any one of claims 1 to 9.