KR102522982B1 - Cosmetics composition containing soft corals distillation extraction - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin soothing comprising a distilled soft coral extract and a method for producing the same, wherein the cosmetic composition contains, as an active ingredient, a distilled soft coral extract prepared by subjecting a soft coral extract to a dew-poll method and one or more specific additives. When contained together, the skin irritation is very low and the soothing effect is remarkably high, and the cosmetic effects such as anti-inflammatory, whitening, and antioxidant are very excellent, so it can be usefully used in functional cosmetics.

Description

Cosmetic composition containing soft coral distillation extract {COSMETICS COMPOSITION CONTAINING SOFT CORALS DISTILLATION EXTRACTION}

The present invention relates to a cosmetic composition comprising distilled soft coral extract as an active ingredient and a method for preparing the same.

The ocean covers more than 70% of the earth's surface and is rich in a variety of marine life including plants, animals and microorganisms. Coral, one of these marine organisms, is called the 'flower of the sea' for its splendid and beautiful appearance and has been commercialized as jewelry or accessories in the past. As a result, it is attracting attention as an important material in the medical field, such as drug development and artificial bones using coral skeletons.

On the other hand, as interest in the skin has recently increased, the demand for cosmetics with various functions such as skin hydration, pore reduction, skin exfoliation, wrinkle relief, and whitening effect is steadily increasing. Accordingly, functional cosmetics with various functions are increasing. Research on is continuing.

As an example, a composition for inhibiting the expression of inflammation-related factors, cytokines, and related proteins using an ethanol-extracted soft coral has been developed for the prevention or treatment of inflammatory diseases of the skin. However, the composition has an excellent anti-inflammatory effect and is not toxic to macrophages, but has a limit in application to actual cosmetic products due to skin irritation.

Republic of Korea Patent Publication No. 10-2018-0060754

Accordingly, an object of the present invention is to provide a cosmetic composition comprising, as an active ingredient, a distilled soft coral extract having low skin irritation and an excellent soothing effect using the soft coral extract, and a method for preparing the same.

In order to solve the above problems,

The present invention, in one embodiment, soft coral distillation extract; And it provides a cosmetic composition for skin soothing comprising at least one additive.

At this time, the soft coral distilled extract is derived from at least one soft coral selected from the group consisting of Dendronephthya spinulosa, Dendronephthya gigantea, Pink sea mandrami (Scleronephthya gracillimum) and Red sponge mandrami (Chromonephthea hirotai) may have been

In addition, the content of the distilled soft coral extract may be 1.0 to 50.0 wt.% based on the total cosmetic composition.

In addition, the additive may include at least one selected from the group consisting of panthenol, allantoin, dipotassium glycyrrhizate, 1,2-hexanediol, and a plant extract, wherein the plant extract is an aloe leaf extract , Centella asiatica extract, eoseongcho extract, tea tree extract, and witch hazel extract may include one or more selected from the group consisting of.

In addition, when the additive includes at least one of panthenol, allantoin, dipotassium glycyrrhizate, and 1,2-hexanediol, the content of the additive may be 0.1 to 10.0 wt.% based on the total cosmetic composition.

Specifically, the cosmetic composition is soft coral distilled extract 1.0 ~ 50.0 wt.%; Plant extract 45.0 ~ 55.0 wt.%; and 1.0 to 3.0 wt.% of 1,2-hexanediol; or

Soft coral distillate extract 1.0-50.0 wt.%; Panthenol 0.1-2.0 wt.%; Allantoin 0.1-2.0 wt.%; Dipotassium glycyrrhizate 0.1-2.0 wt.%; and 1.0 to 3.0 wt.% of 1,2-hexanediol.

In addition, the present invention, in one embodiment, the distillation step of distilling and cooling the diluted soft coral extract at least once to prepare a distilled soft coral extract (S1); and the soft coral distillate extract; And a blending step (S2) of preparing a cosmetic composition by blending one or more additives; provides a method for producing a cosmetic composition for soothing skin.

At this time, in the dew removal step (S1), when distillation and cooling are repeated twice or more, the dilution rate of the diluted solution is lowered by 20 to 40% from the dilution rate of the previous step, and the evaporation rate of the diluted solution is reduced to the evaporation rate of the previous step. 10 to 30% lower than that can be performed.

In addition, after the mixing step (S2), a step of aging the cosmetic composition prepared may be further included.

The cosmetic composition according to the present invention contains, as an active ingredient, a distilled soft coral extract prepared by performing the dewpo method of soft coral extract together with one or more specific additives, so that the skin irritation is very low and the soothing effect is remarkably high, and anti-inflammatory , whitening, anti-oxidation and other cosmetic effects are very excellent, so it can be usefully used in functional cosmetics.

Prior to describing the present invention in detail below, it is understood that the terms used herein are intended to describe specific embodiments and are not intended to limit the scope of the present invention, which is limited only by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art unless otherwise specified.

Throughout this specification and claims, the terms "comprise", "comprise" and "comprising", unless stated otherwise, are meant to include a stated object, step or group of objects, and steps, and any other object However, it is not used in the sense of excluding a step or a group of objects or a group of steps.

In the present invention, the use of "or" or "and" means "and/or" unless otherwise indicated. In the present invention, "A to B" representing a range herein means a range of A or more and B or less.

In addition, in the present specification, "active ingredient" is a component that exhibits the desired activity alone or can exhibit activity together with a carrier having no activity itself. It refers to a component that exhibits cosmetic effects such as wrinkle improvement, anti-inflammatory, and antioxidant.

Furthermore, in the present specification, "isulpoje" means a method of distilling and extracting the prepared extract. That is, in the case of the soft coral isulpoje extract used in the present invention, it may mean that the soft coral is distilled and extracted twice.

On the other hand, various embodiments of the present invention can be combined with any other embodiments unless clearly indicated to the contrary. Any feature indicated as being particularly desirable or advantageous may be combined with any other features and characteristics indicated as being particularly desirable or advantageous. Hereinafter, embodiments of the present invention and effects thereof will be described with reference to the accompanying drawings.

Hereinafter, the present invention will be described in detail.

cosmetic composition

The present invention, in one embodiment, soft coral distillation extract; And it provides a cosmetic composition for skin soothing comprising at least one additive.

The cosmetic composition according to the present invention contains distilled soft coral extract and one or more specific additives, so that skin irritation is low and soothing effect is high, and anti-inflammatory, whitening, anti-oxidation, etc. can exhibit excellent cosmetic effects.

At this time, the soft coral distillation extract is an extract obtained by performing the dewpo method on the soft coral extract, and the soft coral extract is a spiny resin cockscomb, a large resin cockscomb (Dendronephthya gigantea), a pink sea cockscomb (Scleronephthya gracillimum) and a red sponge mandrami (Chromonephthea hirotai) may be derived from one or more types of soft corals selected from the group consisting of.

Specifically, the soft coral extract may be derived from one of the thorny cockscombs, the large resin cockscombs, the pink sea cockscombs, and the red sponge cockscombs, or derived from two, three, or four of the above-mentioned soft corals. there is.

In addition, the content of the distilled soft coral extract may be 1.0 to 50.0 wt.%, specifically 1.0 to 20.0 wt.%, based on the total cosmetic composition; 10.0-30.0 wt.%; 5.0 to 15.0 wt.%; 10.0-30.0 wt.%; 20.0 to 50.0 wt.%; or 8.0 to 12.0 wt.%.

In addition, the at least one additive may include at least one selected from the group consisting of panthenol, allantoin, dipotassium glycyrrhizate, 1,2-hexanediol, and a plant extract, and the plant extract may include an aloe leaf extract , Centella asiatica extract, eoseongcho extract, tea tree extract, and witch hazel extract may include one or more selected from the group consisting of.

Here, panthenol is an alcohol derivative of pantothenic acid and is a long-acting pantothenic acid agent. It is oxidized in vivo to become pantothenic acid and is effective, and is mainly used as a hair conditioning agent, humectant, softener, moisturizer, etc.

In addition, allantoin is a chemical debrider of necrotic and scab tissue, from anti-acne products, sun protection products, clarifying lotions to dead skin cells due to its ability to help heal minor wounds and promote healthy skin. It is used in a variety of products, including skin-softening cosmetic formulations due to its keratolytic effect and abrasive and astringent properties.

In addition, 1,2-hexanediol is used as a preservative for skin external preparations.

Furthermore, in plant extracts, aloe leaf extract has skin regeneration and skin soothing effects and is used in anti-acne products, etc. Centella asiatica extract is also called centella extract, has an auxiliary treatment effect for wounds and skin ulcers, and binds blood vessels It is known to strengthen tissues and increase collagen formation.

In addition, Eoseongcho extract and tea tree extract are known to be highly effective in improving atopic or acne-prone skin.

Furthermore, the content of the additive may be 0.5 to 60 wt.% based on the total cosmetic composition. More specifically, when the additive includes at least one of panthenol, allantoin, dipotassium glycyrrhizate and 1,2-hexanediol, 0.1 to 10.0 wt.% or 0.1 to 5.0 wt. %; In the case of including the plant extract, it may be 10.0 to 60.0 wt.%, 10.0 to 15.0 wt.%, 35.0 to 59.9 wt.% or 15.0 to 49.9 wt.% based on the total cosmetic composition.

As an example, the cosmetic composition according to the present invention contains 1.0 to 50.0 wt.% of distilled soft coral extract; Plant extract 45.0 ~ 55.0 wt.%; and 1.0 to 3.0 wt.% of 1,2-hexanediol.

As another example, the cosmetic composition may include 1.0 to 50.0 wt.% of distilled soft coral extract; Panthenol 0.1-2.0 wt.%; Allantoin 0.1-2.0 wt.%; Dipotassium glycyrrhizate 0.1-2.0 wt.%; and 1.0 to 3.0 wt.% of 1,2-hexanediol.

The cosmetic composition according to the present invention can significantly improve anti-inflammatory, whitening, and antioxidant effects as well as skin soothing effects by maximizing the synergy between each component by controlling the contents of the distilled soft coral extract and the additives in the above range.

On the other hand, the cosmetic composition according to the present invention may further include purified water together with a distilled soft coral extract and one or more additives, and at this time, the content of the purified water may be included as much as the remaining amount remaining after including the distilled soft coral extract and one or more additives. . For example, the content of the purified water may be 30.0 to 90.0 wt.%, specifically 30.0 to 90.0 wt.%; 30.0 to 50.0 wt.%; 40.0-60.0 wt.%; 34.0-39.0 wt.%; 35.0-40.0 wt.%; or 85.0 to 90.0 wt.%; it may be.

functional cosmetics

On the other hand, the present invention, in one embodiment, provides a functional cosmetic comprising the cosmetic composition of the present invention described above.

The functional cosmetic according to the present invention includes a cosmetic composition containing a distilled soft coral extract and one or more additives, and exhibits excellent anti-inflammatory, whitening, and antioxidant effects along with a remarkably excellent skin soothing effect, so that it can be easily used as a functional cosmetic. can

Here, the functional cosmetics include essence, ampoule, soap, tonic and body essence, emulsion, lotion, cream (oil-in-water type, water-in-oil type, multi-phase) as skin and body care cosmetics containing the cosmetic composition of the present invention, It can be prepared in various formulations such as solutions, suspensions (anhydrous and aqueous), anhydrous products (oil and glycol-based), gels, powders, etc., and the cosmetic composition is included in an amount of 0.05 to 100 wt% with respect to the total cosmetic product, and the ampoule formulation In the case of, it may be made of 100% of the functional composition.

In addition to the cosmetic composition of the present invention, the functional cosmetic may include an acceptable carrier in skin cosmetic formulations.

Alcohols, oils, surfactants, fatty acids, silicone oils, preservatives, humectants, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, coloring agents, fragrances, diluents, and the like may be exemplified as the carrier.

Specific compounds or compositions that can be used as the alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion, stabilizer, sunscreen, coloring agent, fragrance, diluent, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate compound or composition.

Here, to give some examples, water-soluble polyhydric alcohols such as higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, sorbitol, polyethylene glycol, etc. are mentioned as alcohols, and avogard oil, palm oil, beef tallow as oils , jojoba oil, etc., examples of preservatives include ethyl paraben, butyl paraben, etc., moisturizing agents include hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate, etc., and diluents include ethanol, isopropanol etc. can be mentioned.

More specifically, when the formulation of the functional cosmetic is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. can be used.

In addition, when the formulation is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / propellants such as butane or dimethyl ether.

In addition, when the formulation is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

In addition, when the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , aluminum metahydroxide, bentonite, agar or tracanth, and the like can be used.

In addition, when the formulation is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, fatty alcohols, fatty acid glycerides, fatty acid diethanol amides, vegetable oils, linolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

In addition, in the case of soap containing the cosmetic composition according to an embodiment of the present invention, it may be prepared by including the cosmetic composition in a soap base, and may be prepared by including a skin moisturizer, an emulsifier, a water softener, etc. as auxiliary additives. .

Vegetable oils such as palm oil, palm oil, soybean oil, castor oil, olive oil, and palm kernels, or animal oils such as beef tallow, lard, brisket, and fish oil may be used as the soap base material, and as the skin moisturizer, glycerin, erythritol, polyethylene glycol , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol, etc. may be used. Examples of the emulsifier include natural oils, waxy fatty alcohols, hydrocarbons, natural plant extracts, etc. may be used, and tetrasodium EDTA may be used as the water softening agent.

In addition, the soap composition of the present invention may additionally include an antibacterial agent, a dispersing agent, a foam inhibitor, a solvent, an antiscaling agent, a corrosion inhibitor, a fragrance, a colorant, a metal ion sequestering agent, an antioxidant, a preservative, and the like as auxiliary additives.

Manufacturing method of cosmetic composition

Furthermore, the present invention, in one embodiment,

A distillation step (S1) of preparing a distilled soft coral extract by distilling and cooling the diluted soft coral extract at least once; and

said soft coral distilled extract; And a blending step (S2) of preparing a cosmetic composition by blending one or more additives; provides a method for producing a cosmetic composition for soothing skin.

The manufacturing method of the cosmetic composition according to the present invention is a method of distilling and cooling the soft coral extract, that is, by performing a dew blowing method on the soft coral extract to prepare a soft coral distilled extract (S1), It is performed in a way to prepare a cosmetic composition by blending additives (S2).

At this time, the dew removal step (S1) of the soft coral extract may be performed by distilling and cooling the diluted solution obtained by diluting the soft coral extract one or more times. Specifically, the distillation extraction step (S2) is a step of obtaining a liquid extract by heating a diluted solution obtained by diluting the concentrated soft coral extract and cooling the material containing the vaporized active ingredient, wherein the diluted solution has a reduced volume with respect to the initial volume. After vaporizing until the ratio is 30 to 50%, a liquefied liquid extract can be obtained by passing the evaporated steam through a pipe in which cooling water at 15 to 25 ° C circulates.

At this time, the dilution solution may use water containing purified water or distilled water, a lower alcohol having 1 to 4 carbon atoms such as methanol, ethanol, butanol, or a mixed solvent thereof as a dilution solvent. As an example, purified water or distilled water may be used as the diluent.

In addition, the concentrated soft coral extract may be diluted in distilled water at a concentration of 5 wt.% to 50 wt.%, specifically, the soft coral extract is 5 wt.% to 30 wt.%; 10 wt.% to 25 wt.%; 15 wt.% to 25 wt.%; Or it may be diluted to a concentration of 20 wt.% to 40 wt.%.

In addition, the distillation temperature of the distillation extraction step (S2) may be 50 ℃ to 150 ℃, specifically 70 ℃ to 130 ℃; 80° C. to 120° C.; Or it may be 90 ℃ to 110 ℃.

In addition, the distillation extraction step (S2) is 0.5 atm to 5.0 atm; Alternatively, it may be performed for 30 minutes to 600 minutes at a pressure of 0.5 atm to 1.5 atm. The present invention prevents damage to active ingredients that are weak to heat by controlling the distillation temperature, pressure, and execution time in the above-described range during the dew removal step (S2), while extracting active ingredients that are difficult to extract under general distillation conditions in a short time at low temperature and low pressure. can be extracted efficiently.

In addition, in the distillation extraction step (S2), when distillation and cooling are repeated twice or more, the dilution rate of the diluted solution is lowered to 20 to 40% lower than the dilution rate of the previous step, and the evaporation rate of the diluted solution is reduced to that of the previous step. It may be repeatedly performed in a manner that lowers the evaporation rate to 10 to 30%. For example, the first distillation extract can be obtained by diluting the soft coral extract to a concentration of 20 wt.%, vaporizing the diluted solution until it is reduced to 50% of the original volume, and then cooling. Then, the first distillation extract was obtained continuously, and the remaining diluted solution was secondly diluted in distilled water at a concentration of 24 to 28 wt.%, vaporized until the volume of the second diluted solution reached 55 to 65%, and cooled. A tea distillation extract can be obtained.

In this way, when the distillation extraction step (S2) is repeated twice or more, specifically, 2 to 5 times, the concentration of the active ingredient contained in the prepared soft coral distilled extract is relatively high, so the cosmetic effect can be increased. there is.

In addition, the distilled soft coral extract prepared in this way may be combined with one or more additives (S2) to prepare a cosmetic composition according to the present invention.

In this case, the content of the distilled soft coral extract may be 1.0 to 50.0 wt.%, specifically 1.0 to 20.0 wt.%, based on the total cosmetic composition; 10.0-30.0 wt.%; 5.0 to 15.0 wt.%; 10.0-30.0 wt.%; 20.0 to 50.0 wt.%; or 8.0 to 12.0 wt.%.

In addition, the at least one additive may include at least one selected from the group consisting of panthenol, allantoin, dipotassium glycyrrhizate, 1,2-hexanediol, and a plant extract, and the plant extract may include an aloe leaf extract , Centella asiatica extract, eoseongcho extract, tea tree extract, and witch hazel extract may include one or more selected from the group consisting of.

In addition, the content of the additive may be 0.5 to 60 wt.% based on the total cosmetic composition, and more specifically, the additive is at least one of panthenol, allantoin, dipotassium glycyrrhizate, and 1,2-hexanediol. When formulated, it may be 0.1 to 10.0 wt.% or 0.1 to 5.0 wt.% based on the total cosmetic composition; In the case of including the plant extract, it may be 10.0 to 60.0 wt.%, 10.0 to 15.0 wt.%, 35.0 to 59.9 wt.% or 15.0 to 49.9 wt.% based on the total cosmetic composition.

As an example, the cosmetic composition according to the present invention contains 1.0 to 50.0 wt.% of distilled soft coral extract; Plant extract 45.0 ~ 55.0 wt.%; and 1,2-hexanediol at 1.0 to 3.0 wt.%.

As another example, the cosmetic composition may include 1.0 to 50.0 wt.% of distilled soft coral extract; Panthenol 0.1-2.0 wt.%; Allantoin 0.1-2.0 wt.%; Dipotassium glycyrrhizate 0.1-2.0 wt.%; and 1,2-hexanediol at 1.0 to 3.0 wt.%.

The cosmetic composition according to the present invention can significantly improve anti-inflammatory, whitening, and antioxidant effects as well as skin soothing effects by maximizing the synergy between each component by controlling the contents of the distilled soft coral extract and the additives in the above range.

On the other hand, the manufacturing method of the cosmetic composition according to the present invention may further include a step (S3) of aging the cosmetic composition prepared after the mixing step (S2).

More specifically, the aging step (S3) is a step of aging for at least one month after filtering the blended cosmetic composition, and the activity of the active ingredient can be further improved by aging the distilled soft coral extract blended with one or more additives. .

Furthermore, the present invention is a formulation step (S40) of preparing a cosmetic of a desired formulation by mixing the cosmetic composition with ingredients constituting other cosmetics (eg, auxiliary additives) to prepare functional cosmetics after the aging step (S3) ) may be included.

In the formulation step (S40) according to the present invention, cosmetics such as essence, lotion, cream, and ampoule may be prepared by mixing the cosmetic composition of the present invention and other cosmetic ingredients.

Hereinafter, Preparation Examples, Examples and Comparative Examples are merely to illustrate the present invention, and the contents of the present invention are not limited thereto.

Manufacturing Examples 1 to 3. Preparation of soft coral distillate extract

Prickly resin mandrami, large resin mandrami, pink sea cockscomb, and red sponge mandrami were collected, washed, frozen, and pulverized to prepare soft coral in powder form. Then, as shown in Table 1 below, they were each weighed, put into a reactor together with 85 g of purified water, stirred at 60 ± 2 ° C for 3 hours, filtered and concentrated to prepare a soft coral extract.

The prepared soft coral extract was diluted in purified water to a concentration of 20 wt.% to prepare a diluted solution, which was injected into a reflux condenser. Then, the distilled soft coral extract was prepared by heating the diluted solution to 100 ± 2 ° C., followed by first or second distillation and cooling (pressure: 1.0 ± 0.2 atm).

Example 1 Example 2 Example 3 soft coral large resin mendrami 6g 15g 10g Prickly resin mendrami 3g - 5g pink sea cockscomb 3g - 5g Red Sponge Mandrami 3g - 5g Primary
Iseulpoje dilution rate 20% 20% 15% evaporation rate 50% 50% 30% Secondary
Iseulpoje dilution rate - - 25% evaporation rate - - 60%

Preparation Example 4.

Equal amounts of four types of soft corals were prepared in the same manner as in Preparation Example 1, and they were introduced into a reactor together with 300 g of 80 vol.% ethanol and refluxed at 80±1° C. to prepare an ethanol reflux extract.

Experimental Example 1. Efficacy evaluation of soft coral distillation extract

In order to evaluate the efficacy of the distilled soft coral extract used in the present invention, the following experiments were performed on the distilled soft coral extracts prepared in Preparation Examples 1 to 4.

(1) skin irritation

A patch containing the distilled soft coral extract prepared in Preparation Example as an active ingredient was prepared, and a human skin primary irritation test was conducted for 48 hours using the patch.

Specifically, the distilled soft coral extract prepared in Preparation Example was diluted in purified water to a concentration of 10 wt.%, and a patch (2 × 4 cm) containing the test substance was applied to clinical subjects for 6 hours, and then the patch has been removed. Then, after 24 hours, the skin irritation level was evaluated by observing the patch-attached area. At this time, a total of 20 subjects, 10 women and 10 men each in their 10s to 50s, were selected as the clinical subjects. In addition, the evaluation criteria for skin irritation are as follows, and the results are shown in Table 1:

Non-irritating: Sensitization value 0.00 or more and less than 0.15

Mild irritation: Sensitization value 0.15 or more and less than 0.70

Medium irritation: Sensitization value 0.70 or more and less than 1.00

Strong stimulation: Sensitization value over 1.00

(2) Measurement of whitening effect

In order to confirm the whitening effect of the cosmetic composition according to the present invention, the inhibitory activity of tyrosinase used as an enzyme to form melamine was measured.

First, the inhibitory activity of tyrosinase of the distilled soft coral extract prepared in Preparation Example was measured using mouse pigment cells (B16 melanoma cells) (Korea Cell Line Bank), and as a control, it is known to inhibit melanin production. Intracellular tyrosinase inhibitory activity by arbutin was compared.

Murine melanoma (B-16 F1) cells (Korea Cell Line Bank) were cultured in DMEM medium containing 10% fetal bovine serum (FBS) at 1 × 10 ⁵ per well in a 6-well plate. After inoculation, the cells were incubated under 5% CO ₂ and 37° C. until about 80% or more of the cells adhered to the bottom of the well. Subsequently, the medium was removed, and the distilled soft coral extract prepared in Preparation Example was replaced with a medium diluted to an appropriate concentration, followed by culturing for 3 days under 5% CO ₂ and 37°C. The concentration range of the sample was determined to be 50 ppm, and arbutin and purified water were used as positive and negative controls. The cells from which the medium was removed were washed with PBS (phosphate buffered saline) and treated with trypsin to recover the cells. The recovered cells were counted using a hematocytometer (Tiefe Depth Profondeur 0.100 mm, Paul Marienfeld GmbH & Co.KG, Germany), centrifuged at 5,000 to 10,000 rpm for 10 minutes, and supernatant was removed to obtain pellets. The cell pellet was pulverized using a lysis buffer and centrifuged at 12,000 rpm for 10 minutes to collect the supernatant. The absorbance was measured at 492 nm with a microplate reader (Bio-Tek ELx8081U, USA) to measure the tyrosinase activity per cell constant, and the results are shown in Table 2 below.

(3) Antioxidant effect

In order to confirm the antioxidant effect of the cosmetic composition according to the present invention, the radical removal ability of the sample was measured.

In a mixture of 180 μl of 0.2mM DPPH solution dissolved in methanol and 20 μl of distilled water, the distilled soft coral extract (100 μg / ml) prepared in Preparation Example was put into a 96-well microplate, and incubated at 37 ° C. for 30 minutes, then the reduced The amount of DPPH was measured for absorbance at 520 nm using a spectrophotometer (Precision microplate reader; Molecular Devices, VersaMax), and the activity was compared with a negative control (purified water) to which no sample was added. In addition, α-tocopherol was used as a positive control, and the measured results are shown in Table 2.

(4) Anti-inflammatory effect

In order to confirm the anti-inflammatory effect of the cosmetic composition according to the present invention, NO production inhibitory ability was measured.

First, RAW264.7 cells were dispensed in a 96 well plate at 2×10 ⁶ cells/ml and the cells were cultured for 18 hours, and then the soft coral distilled extract prepared in Preparation Example was treated with 100 μg/ml for 30 minutes, respectively, and 24 incubated for hours. After culturing, LPS was treated at a concentration of 1 μg/ml, and cultured in an incubator under conditions of 37° C. temperature and 5% CO ₂ for 20 hours. After mixing 100 μl of the cultured cell culture medium and 100 μl of Griess reagent (1% sulfanilamide in 5% phosphoric acid and 1% α-naphthylamide in H ₂ O) and reacting at room temperature for 5 minutes, ELISA microplate reader (Tecan, USA) at 540 nm ) to measure the absorbance. After obtaining a standard curve by measuring absorbance with sodium nitrite (NaNO ₂ ), the concentration (%) of NO was calculated from the measured absorbance, and LPS and purified water were used as positive and negative controls, respectively. The measured results are shown in Table 2 below.

Preparation Example 1 Preparation Example 2 Preparation Example 3 Production Example 4 positive control negative control group Whitening Tyrosinase inhibitory ability 64.50% 53.2% 72.8% 23.3% 100.0% 0.00% Standard Deviation 5.20% 3.70% 6.10% 6.43% 7.60% - antioxidant ROS removal ability 57.30% 52.2% 64.6% 27.4% 100.0% 0.00% Standard Deviation 6.20% 5.70% 5.10% 5.86% 5.30% - anti-inflammatory NO inhibition ability 68.70% 65.3% 76.8% 35.1% 100.0% 0.00% Standard Deviation 8.10% 7.3% 7.6% 7.13% 5.3% - stimulation stimulation level 0.14 0.14 0 0.69 2.91 0.42 Judgment non-irritating non-irritating non-irritating light stimulation strong stimulation light stimulation

Examples 1 to 4 and Comparative Examples 1 to 3. Preparation of cosmetic composition

Together with the distilled soft coral extract prepared in Preparation Example 1, panthenol, allantoin, dipotassium glycyrrhizate, 1,2-hexanediol and a plant extract were prepared. At this time, as the plant extract, an aloe leaf extract, centella asiatica extract, houttuynia cordata extract, tea tree extract, and witch hazel extract were prepared. A cosmetic composition was prepared by weighing each of the prepared ingredients as shown in Table 3 below.

Unit: wt.% Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Soft coral distillate extract 10 10 10 10 - - - panthenol - 0.1~2.0 0.1~2.0 - 0.1~2.0 0.1~2.0 - allantoin - 0.05~0.2 0.05~0.2 - 0.05~0.2 0.05~0.2 - dipotassium glycyrrhizate - 0.05~0.2 0.05~0.2 - 0.05~0.2 0.05~0.2 - 1,2-hexanediol 1.5 to 2.5 1.5 to 2.5 1.5 to 2.5 1.5 to 2.5 1.5 to 2.5 1.5 to 2.5 1.5 to 2.5 aloe leaf extract - 10.0 - 10.0 10.0 - 10.0 centella extract - 10.0 - 10.0 10.0 - 10.0 Houttuynia cordata extract - 10.0 - 10.0 10.0 - 10.0 tea tree extract - 10.0 - 10.0 10.0 - 10.0 Witch Hazel Extract - 10.0 - 10.0 10.0 - 10.0 Purified water 87.5 to 88.5
(residual amount) 35.1 to 38.3
(residual amount) 85.1 to 88.3
(residual amount) 37.5 to 38.5
(residual amount) 45.1 to 48.3
(residual amount) 95.1~98.3
(residual amount) 47.5 to 48.5
(residual amount)

Examples 5-7. Preparation of cosmetic composition

It was carried out in the same manner as in Example 3, but each component was weighed and mixed, and then aged twice at 5 to 20 ° C. to prepare a cosmetic composition. At this time, the aging conditions of the cosmetic composition are as shown in Table 4 below.

Example 5 Example 6 Example 7 1st aging (24-36 hours) 5℃ 10℃ 15℃ 2nd aging (12~24 hours) 15℃ 17℃ 20℃

Experimental Example 2. Evaluation of skin soothing effect of cosmetic composition

In order to evaluate the skin soothing effect of the cosmetic composition according to the present invention, the following experiments were performed on the cosmetic compositions of Examples and Comparative Examples.

(1) Erythema relief and skin soothing effect

Targeting 12 men and women in their 20s and 30s who do not currently have skin disease or skin allergy symptoms, after inducing skin irritation through the tape stripping method on the inner part of the lower arm, the irritation caused by applying the prepared cosmetic composition The degree of sedation was evaluated relative to each other.

Specifically, skin erythema was induced by taping 20 to 30 times (the number of times adjusted according to the volunteer's skin sensitivity) with general scotch tape on two inner parts of the lower arm. After inducing erythema, the cosmetic compositions of Examples 1 to 7 and Comparative Examples 1 to 3 were applied, respectively, and purified water was separately applied as a negative control. After 3 hours of application, the degree of skin soothing was evaluated and shown in Table 5 below, and the criteria are as follows:

- 0.0 points: no reaction

- 0.5 points: faint or mild erythema

- 1.0 points: well-defined but weak erythema, edema and papules

- 2.0 points: distinct erythema, papules and vesicles

- 3.0 points: Severe erythema and blisters, scab formation

degree of skin irritation Degree of skin irritation 3 hours after application of the composition left right left Irritation relief rate [%] right Irritation relief rate [%] Example 1 22.0 21.0 11.5 47.7% 11.0 47.6% Example 2 23.0 22.0 9.0 60.9% 9.0 59.1% Example 3 22.0 21.0 9.0 59.1% 9.5 54.8% Example 4 22.5 22.0 12.5 44.4% 13.0 40.9% Example 5 23.0 22.0 6.0 73.9% 6.5 70.5% Example 6 22.5 22.0 8.0 64.4% 8.0 63.6% Example 7 22.0 23.0 8.5 61.4% 9.0 60.9% Comparative Example 1 23.0 21.0 16.0 30.4% 14.0 33.3% Comparative Example 2 24.0 21.0 17.0 29.2% 14.5 31.0% Comparative Example 3 22.0 22.0 17.5 20.5% 16.0 27.3% negative control group
(Purified water) 24.0 22.0 19.5 18.8% 18.5 15.9%

(2) Skin trouble relief and skin soothing effect

A total of 20 subjects, 10 males and females in their 20s and 30s suffering from skin troubles, were selected as test subjects. The selected test subject receives a photo and preliminary evaluation of the area where the skin trouble occurred (hereinafter referred to as "skin trouble area") on the test start date, and applies Example 1 to the skin trouble area twice a day for 4 weeks after the test start date. The cosmetic compositions of ~4 and Comparative Examples 1-3 were applied. After 4 weeks had elapsed, pictures were taken and post-evaluation of the skin trouble area was performed in the same manner as on the start of the test. At this time, the post evaluation criteria are as follows, and the results are shown in Table 6 below:

- 1 point: Trouble area widens and becomes more sensitive

- 2 points: There is no change in the width of the trouble area, but it is somewhat sensitive

- 3 points: no change

- 4 points: The area of the trouble area is reduced to less than 20% of the initial area, and it is somewhat calmed down

- 5 points: The area of the trouble area is reduced by 20% or more from the initial standard, and it is very calming.

Relief of skin troubles 5 points 4 points 3 points 2 points 1 point Sum Example 1 5 7 7 One 0 76 Example 2 10 8 2 0 0 88 Example 3 7 9 4 0 0 83 Example 4 6 9 4 One 0 80 Comparative Example 1 One 7 11 One 0 68 Comparative Example 2 One 5 15 One 0 72 Comparative Example 3 0 8 12 0 0 68 negative control group
(Purified water) 0 0 18 One One 57

The features, structures, effects, etc. illustrated in each of the above-described embodiments can be combined or modified with respect to other embodiments by those skilled in the art in the field to which the embodiments belong. Therefore, contents related to these combinations and variations should be construed as being included in the scope of the present invention.

Claims (11)

comprising a soft coral distillate extract, a plant extract and one or more additives;
The distilled soft coral extract is prepared from two or more types of soft corals selected from the group consisting of Dendronephthya spinulosa, Dendronephthya gigantea, Scleronephthya gracillimum and Chromonephthea hirotai,
The plant extract includes at least one selected from the group consisting of aloe leaf extract, centella asiatica extract, houttuynia cordata extract, tea tree extract, and witch hazel extract,
The additive includes at least one selected from the group consisting of panthenol, allantoin, dipotassium glycyrrhizate, 1,2-hexanediol, and plant extracts,
The cosmetic composition includes 1.0 to 50.0 wt.% of distilled soft coral extract; Plant extract 45.0~55.0 wt.%; Panthenol 0.1-2.0 wt.%; Allantoin 0.1-2.0 wt.%; Dipotassium glycyrrhizate 0.1-2.0 wt.%; And 1,2-hexanediol 1.0 ~ 3.0 wt.% A cosmetic composition for soothing skin.
delete delete delete delete delete delete delete According to claim 1,
The distilled soft coral extract is a cosmetic composition for skin soothing that is prepared by distilling and cooling the distilled liquid obtained by diluting the soft coral extract at least once (S1).
According to claim 9,
In the dew removal step (S1), when distillation and cooling are repeated twice or more, the dilution rate of the diluted solution is lowered by 20 to 40% than the dilution rate of the previous step, and the evaporation rate of the diluted solution is increased by 10 to 40% from the evaporation rate of the previous step. A cosmetic composition for skin soothing that lowers the skin by 30%. delete