KR102520633B1 - Dental membrane filter with improved absorption and manufacturing method thereof - Google Patents

Abstract

The present invention relates to a dental membrane filter with improved absorbency and a manufacturing method thereof. Dental membrane filter with improved absorbency according to an embodiment of the present invention includes a support layer comprising a biodegradable polymer; a shielding layer laminated on an upper surface of the support and containing collagen and a biodegradable polymer; And it may include a polymer film laminated on the upper surface of the shielding layer.

Description

Dental membrane filter with improved absorption and its manufacturing method {DENTAL MEMBRANE FILTER WITH IMPROVED ABSORPTION AND MANUFACTURING METHOD THEREOF}

The present invention relates to a dental membrane filter with improved absorbency and a manufacturing method thereof. More specifically, the present invention is to provide a dental membrane shield with improved absorbability or biodegradability that does not require additional removal surgery while having excellent space retention during bone grafting, and to provide a manufacturing method thereof.

When a tooth is missing, a bridging procedure has been performed in which a denture is inserted or the missing part is covered with metal or the like using a nearby tooth as a pillar. However, such a procedure has several problems, such as that the dentures or bridged artificial teeth have a weak ability to chew food and adversely affect surrounding teeth. Accordingly, implant surgery has appeared as one of the advanced dental procedures. Such an implant procedure forms an artificial tooth root in the alveolar bone and uses it to combine an artificial tooth, which is a final prosthesis manufactured similarly to a real tooth, so that the patient obtains the effect of using the real tooth.

In the above implantation procedure, an implantation groove is formed using a tool such as a drill to implant a fixture in the alveolar bone where a tooth is missing, and tapping is performed as an optional operation for firmly implanting the fixture in the implantation groove. Thereafter, after implanting the fixture in the implantation groove, a cover screw is fastened to prevent foreign substances from penetrating into the fixture, and the gum is covered and sutured to complete the primary surgery. Subsequently, as a secondary operation, after about 3 to 6 months have elapsed, the sutured gum is re-incised and the cover screw is removed from the fixture. After considering the type of abutment to be fastened, the healing abutment is selected contract The healing abutment at this time is to ensure that the gums are formed cleanly on the fixture before fastening the abutment to the fixture, and after approximately 2 to 3 weeks after fastening, after the gum suitable for the abutment procedure is generated, the healing abutment After removing the butment again, the abutment is combined, and the implant procedure is completed by making an artificial tooth pattern and combining the final prosthetic appliance having the shape of a natural tooth on the upper side of the abutment.

In such a general implant procedure, if part of the alveolar bone is missing and it is difficult to sufficiently support the fixture with only the remaining alveolar bone, a procedure is added to allow the bone graft material to function as a new alveolar bone after filling the missing part with a bone graft material. will do That is, a procedure to create alveolar bone that is insufficient as much as the defect is performed, which is called guided bone regeneration (GBR). In order to perform guided bone regeneration, once a fixture is inserted into the alveolar bone and a bone graft such as artificial bone or autogenous bone is filled in the defected area, a dental membrane is used to maintain the required shape of the filled bone graft material. to cover After that, the dental membrane is fixed to a required position by using a predetermined cover member.

Here, the membrane refers to a membrane that secures space to prevent the gum from growing into the part where bone formation is required in dental diseases requiring bone grafting, such as inflammation, trauma, and implantation.

In the case of a dental membrane used for alveolar bone regeneration, it must be in close contact with the root of the tooth during the procedure to have the effect of blocking the damaged area and other surrounding environments and to prevent the downward proliferation of epithelial cells. It is easy to secure space, and tissue regeneration can be achieved.

KR 10-2015-0134441 A KR 10-2011-0065380 A

An object of the present invention is to provide a dental membrane filter that is harmless to the human body and does not require a separate removal procedure while using a biodegradable polymer.

An object of the present invention is to provide a dental membrane filter capable of achieving excellent shielding effect while increasing the decomposition rate of the filter.

An object of the present invention is to provide a dental membrane filter with enhanced anti-inflammatory activity.

In order to achieve the above object, a dental membrane filter having improved absorbency according to an embodiment of the present invention includes a support layer comprising a biodegradable polymer; a shielding layer laminated on an upper surface of the support and containing collagen and a biodegradable polymer; and a polymer film laminated on the upper surface of the shielding layer.

The biodegradable polymer is polyglycolide (PG), polyglycolic acid (PGA), polylactic acid (PLA), polylactic acid (PLLA), poly-D,L-lactic acid (PDLLA), polycaprolactone (PCL), It may be any one selected from polydioxanone (PDO) and mixtures thereof.

In the dental membrane filter with improved absorbency, the polymer film may be thinner than the shielding layer.

In the dental membrane filter with improved absorbency, the support layer; the shielding layer; And the polymer film may be formed with pores.

In the dental membrane filter with improved absorbency, the shielding layer may have spikes formed on one or both sides.

In the dental membrane filter with improved absorbency, the polymer film may further include chitosan and a hydrophilic additive.

A method of manufacturing a dental membrane filter with improved absorbency according to another embodiment of the present invention includes laminating a shielding layer containing collagen and a biodegradable polymer on an upper surface of a support layer made of a biodegradable polymer; and laminating a polymer film on the upper surface of the shielding layer.

According to another embodiment of the present invention, a method for manufacturing a shielding layer for a dental membrane filter with improved absorption is performed by using any one of roller molding, mold molding, or plasma treatment on a shielding layer containing collagen and a biodegradable polymer. It may include forming spikes on at least one surface of the shielding layer.

Hereinafter, the present invention will be described in more detail.

Dental membrane filter with improved absorbency according to an embodiment of the present invention includes a support layer comprising a biodegradable polymer; a shielding layer laminated on an upper surface of the support and containing collagen and a biodegradable polymer; and a polymer film laminated on the upper surface of the shielding layer.

In the present specification, when it is said to "include" a certain component, it may mean that it may further include other components unless otherwise stated.

Terms such as "on" or "on top" and "on the bottom" or "below" may be understood to describe the relative positional relationship between components or elements, and may be understood as "located above" or "underside". The term "located" may represent a relative positional relationship in a state of being in contact with a specific object as well as in a non-contact state.

As used herein, "collagen" is a natural biodegradable polymer and refers to a protein that is the main constituent of animal connective tissue (skin, bone, tendon, etc.). In this case, it may mean a protein family rather than a specific single protein. More specifically, collagen is a structural support of the body constituting the flesh and connective tissue of mammals, and its basic constituent unit is tropocollagen protein (consisting of three types of polypeptide chains having the same size), and the chains are Each is wound around one another to form a superhelical cable or triple-helix structure.

The membrane filter can achieve the effect of rapidly degrading while achieving the purpose of shielding by reducing the content of biodegradable polymer and collagen.

Preferably, the support layer may have a density of 0.6 to 1.3 g/cm ³ . In general, when the density of the support layer is increased, it is possible to manufacture a thinner membrane filter by increasing its rigidity. there is a problem. However, in the case of the dental membrane filter, considerable rigidity can be achieved while using a thin filter by the spike structure formed on the shielding layer, and a relatively excellent biodegradation effect can be obtained even in a relatively high density range by forming a very thin support layer with high density. can be achieved

On the other hand, when the support layer is lower than the density range, there is a problem in that the entire structure cannot be stably supported while resisting the pressure transmitted by the spike structure of the shielding layer described later.

In addition, -OH groups may be cross-linked on both sides of the support layer. In the case of the cross-linking, it is possible to achieve an effect of increasing degradation resistance within the bone regeneration period by protecting collagen, in particular, the spike structure on the upper surface of the supporting layer. The -OH crosslinking may be performed by a heat or chemical method according to a conventional method in the related field, or may be performed using an additive.

Preferably, the shielding layer is a mixture of collagen and a biodegradable polymer, and may have a plurality of spikes formed on one or both sides.

The spike referred to in the present invention refers to a columnar structure formed on the surface of the shielding layer, and its shape includes all columns of a cylinder, a triangular column, and other shapes.

The spikes can exhibit excellent durability while supporting the shielding layer between the support layer and the polymer film. In particular, it is not biodegradable in the osteogenesis step and maintains high durability and shielding effect for a certain period of time, but after osteogenesis, the membrane filter is rapidly biodegraded, and has the advantage of effectively removing problems such as inflammation and other side effects after the procedure. That is, it maintains high shielding power during the shielding period with excellent durability, and can achieve the effect of rapidly biodegrading after bone formation.

Preferably, the spikes may be formed on both sides of the shielding layer. The spikes formed on both sides maintain high durability with an excellent support effect for the entire film, and the effect of biodegradation at a certain point in time can appear more quickly.

The method for manufacturing a shielding layer for a dental membrane filter includes forming spikes on one or more surfaces of the shielding layer by using any one of roller molding, mold molding, or plasma treatment on a shielding layer containing collagen and a biodegradable polymer. it may be More preferably, the spike structure may be formed while compressing the shielding layer by roller molding.

Preferably, the polymer film may have a thickness of 0.05 to 0.1 μm and a density of 0.6 to 1.3 g/cm ³ . When the polymer film protects the spike surface of the shielding layer and the treatment site, it may protect the shielding film. In addition, biodegradability and overall durability of the membrane filter can be increased while maintaining a relatively high density with a thin thickness by the spike structure formed on the shielding layer. In addition, when the density of the polymer film is less than the above range, there is a problem that the shielding layer including the aforementioned spike structure cannot be stably supported.

The biodegradable polymer is polyglycolide (PG), polyglycolic acid (PGA), polylactic acid (PLA), polylactic acid (PLLA), poly-D,L-lactic acid (PDLLA), polycaprolactone (PCL), It may be any one selected from polydioxanone (PDO) and mixtures thereof.

In the dental membrane filter with improved absorbency, the polymer film may be thinner than the shielding layer.

Since the shielding layer supports the entire membrane film and increases durability while the support layer and the polymer film are spaced apart through the spike, the shielding layer should be thicker than the polymer film.

In the dental membrane filter with improved absorbency, the support layer; the shielding layer; And the polymer film may be formed with pores.

Preferably, the shielding layer may be treated or coated with a natural product exhibiting an anti-inflammatory effect. That is, in the shielding layer, a spaced space between the spike pillars is formed between the shielding layer and the support layer and the polymer film in contact with each other through the spike structure. At this time, as the natural product is treated or applied to the space, the bearing capacity of the shielding layer is increased, and the natural product flows out at regular intervals through the pores to achieve an anti-inflammatory effect. In particular, while providing the natural product in the form of a liquid, gel, powder, paste, etc. through a thickening agent or other additives to increase the viscosity or hardening, the anti-inflammatory effect can be steadily exhibited for a certain period of time.

Preferably, the natural product may include an achinacea extract.

Echinacea (Purple Coneflower) has about nine species worldwide, originating in the United States and later introduced to Europe. In China, about three species were introduced and cultivated in Beijing, Shenyang, and Shandong, and about three species are used as medicine. Echinacea is native to central North America and is rarely found in the wild. It is cultivated in the central and eastern regions of the United States and Europe.

In the case of including the achinacea extract, the relief effect on inflammation caused by the implant procedure may be excellent. In particular, it is possible to increase the anti-inflammatory effect through excellent antibacterial activity against oral microorganisms.

Inflammatory response is a reaction in which the injured area becomes open, red, swollen, and painful. The inflammatory response is initiated by cytokines secreted by activated macrophages while preying on bacteria entering through the wound, including histamine released from mast cells at the wound site.

More specifically, NF-kB, a transcription factor related to the inflammatory response, is translocated to the nucleus by inflammatory stimuli such as LPS, and is activated by inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and interleukin-1β (IL-1β). ) and induces the expression of pro-inflammatory genes such as TNF-α (tumor necrosis factor-α).

The inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) are associated with the production of nitric oxide (NO) and prostaglandin E2 (PGE ₂ ), which are pro-inflammatory mediators, respectively. That is, the cells in which the inflammatory response is induced by LPS promote the expression of iNOS (inducible NO synthase) gene and COX-2 (cyclooxygenase-2) gene, and the genes are NO (nitric oxide) and prostaglandin E2 (PGE ₂ ) genes, respectively. ), thereby causing an inflammatory response.

The NO (Nitric oxide) inhibits the growth of osteoblasts, increases cell death, and induces differentiation of osteoclasts. On the other hand, the prostaglandin E2 (PGE ₂ ) produced during the inflammatory response also induces the differentiation of osteoclasts.

In addition, tumor necrosis factor-α (TNF-α) and interleukin-1 (IL-1) are factors that promote the production of osteoclasts and induce bone resorption. More specifically, the TNF-α and IL-1β generated from NF-kB signaling not only inhibit bone formation from osteoblasts, but also increase the expression of the receptor activator of NF-kB ligand (RANKL), Accordingly, the secreted NF-kB ligand (RANKL) induces the formation of osteoclasts and accelerates alveolar bone resorption and periodontal disease.

As a result, by appropriately controlling pro-inflammatory mediators and pro-inflammatory cytokines, it is possible to regulate osteoclast formation and bone resorption, and the composition containing the rosemary extract as an active ingredient is treated with LPS (Lipopolysaccharides) to reduce inflammation. By suppressing the production of the pro-inflammatory mediator and pro-inflammatory cytokine in MC3T3-E1 osteoblast cells that induced the condition, the anti-inflammatory effect on peri-implantitis can be improved and successful osseointegration of the implant surface can be induced. .

More preferably, the composition comprises Achinacea extract; acai berry extract; It may include any one selected from the group consisting of Arborvitae extract and mixtures thereof.

Acai Berry is a palm fruit that grows near the Amazon rain forest in northern Rajil. This palm tree is a unique palm tree among the approximately 2,500 species of palm trees found worldwide. The acai berry is so famous that Brazilian natives call it the 'fruit of the tree of life'. The round-shaped acai berry has delicious juice and bead-like seeds. The taste is a combination of strawberry and chocolate, with a sweet and unique taste. It also looks good visually because it has a purple color. It bears fruit all year round, but it bears more fruit from July to December.

Arborvitae (Platycladus orientalis) is an evergreen tree, also called Gangbaek, native to China and widely distributed throughout Korea. It can be up to 20m tall and up to 1m in diameter. It is mainly distributed naturally in limestone areas such as Danyang, Chungcheongbuk-do and Andong, Gyeongbuk, and can be called an indicator plant in limestone areas. The small, flat leaves of the cypress tree are overlapped side by side like scales, and egg-shaped female and male flowers bloom on the same tree in April. There are several cultivars cultivated for ornamental purposes, such as snow arborvitae with large branches spreading sideways, pyramidal western arborvitae, golden arborvitae with golden leaves, and round arborvitae with a round tree shape. Arborvitae leaves are known to be effective in women's hemorrhage, or bleeding in the large intestine or rectum, and to prevent high blood pressure and stroke. It has been reported to be effective. In addition, there are flavonoids, essential oil, lead, tannins, and resins in the leaves, and flavonoids include quercetin, kaempferol, myricetin, hinokiflavone, and amentoflavone.

More preferably, the composition may include an achenesia extract, and 20 to 40 parts by weight of an acai berry extract and 10 to 20 parts by weight of a cypress tree extract based on 100 parts by weight of the achinacea extract. In the case of the above range, the production of pro-inflammatory mediators and pro-inflammatory cytokines in MC3T3-E1 osteoblasts, which were treated with LPS (Lipopolysaccharides) to induce an inflammatory state, can be effectively suppressed through synergistic effect by interaction. there is. Through this, it is possible to exhibit effective antibacterial activity against microorganisms in the oral cavity with excellent anti-inflammatory activity at a lower content.

More preferably, the composition includes an akinesia extract, and 20 to 40 parts by weight of an acai berry extract based on 100 parts by weight of the akinesia extract; 10 to 20 parts by weight of cypress tree extract; 5 to 10 parts by weight of sage extract; And it may contain 10 to 20 parts by weight of Zanja extract.

In the case of the above range, additional synergistic activity is observed as a synergistic effect due to the interaction. That is, it is possible to maximize the anti-inflammatory effect on MC3T3-E1 osteoblasts that induce an inflammatory state and the antibacterial effect on microorganisms in the oral cavity. On the other hand, when the value is less than or exceeds the above range, there is a problem in that the effect is significantly reduced.

Sage is also called medicinal salvia. Sage is also known as Common Sage, Garden Sage, and is commonly called Salvia. The stem is square, the lower part is almost woody, the height is 30 to 90 cm, and the whole is fragrant. The leaves are opposite, the petiole is short, the long oval shape, the end is dull and the edge is flat. The back side of the leaf is grayish green with white hairs along with the stem, and the surface has fine wrinkles. Flowers bloom from May to July, purple, raceme attached in layers at the end of branches, and the top is turned. The flowers contain nectar and are a source of honey for bees. The calyx tube is bell-shaped, the corolla is 1.5 to 2 cm long, and the two flowers above and below split like two upper and lower lips at the tip of the wide tube. There are two stamens, and the style comes out longer than the upper lip of the corolla. There are many varieties, some with blue and white flowers.

Zandae is also called Takju, and in Chinese characters it is called Sasam (沙蔘). Its scientific name is Adenophora triphilla var. japonica HARA. am. It is a plant commonly grown in the wild and wild, reaching 40 to 120 cm in height, with thick roots and hairs all over. Radical leaves have long stems and are centrifugal, and disappear when flowers bloom. Cauline leaves grow alternately or face each other, have no petiole, and are oval, obovate, and lanceolate. The length is 4 to 8 cm, the width is 5 to 40 mm, and both ends are narrowed and there is a mount. The flower is a bell-shaped whole flower, blooms in purple in July to September, and forms a sloppy panicle at the end of the main stem. The fruit is capsule and matures in October.

The term 'extract' used herein has a meaning commonly used in the art as a crude extract, but also includes fractions obtained by further fractionation of the extract in a broad sense. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatography (made for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the extract of the present invention.

The extract used in the present invention may be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

As used herein, the term "comprising as an active ingredient" refers to an amount sufficient to affect a desired biological effect, such as beneficial results, including but not limited to prevention, reduction, alleviation or elimination of signs or symptoms of a disease or disorder. , and this amount is referred to as the “effective amount”. Thus, the total amount of each active ingredient in the composition or method is sufficient to yield a significant individual benefit. Thus, the effective amount will depend on the circumstances in which it is being administered. An effective amount can be administered prophylactically or therapeutically.

In addition, if necessary, the natural product may be used by applying it to a general membrane filter as well as a spiked shielding layer.

In the dental membrane filter with improved absorbency, the polymer film may further include chitosan and a hydrophilic additive.

When the chitosan and the hydrophilic additive are further included, durability of the membrane filter and biodegradability can be adjusted within a certain range.

A method of manufacturing a dental membrane filter with improved absorbency according to another embodiment of the present invention includes laminating a shielding layer containing collagen and a biodegradable polymer on an upper surface of a support layer made of a biodegradable polymer; and laminating a polymer film on the upper surface of the shielding layer.

According to another embodiment of the present invention, a method for manufacturing a shielding layer for a dental membrane filter with improved absorbency is to spike a shielding layer containing collagen and a biodegradable polymer on one or more surfaces of the shielding layer through roller molding or mold molding. It may include a forming step.

The present invention provides a dental membrane filter that is harmless to the human body while using a biodegradable polymer and does not require a separate removal procedure.

The present invention provides a dental membrane filter capable of achieving excellent shielding effect while increasing the decomposition rate of the filter.

The present invention provides a dental membrane filter with enhanced anti-inflammatory activity.

1 is a flowchart showing a manufacturing method according to an embodiment of the present invention.
Figure 2 shows a flow chart showing a manufacturing method according to an embodiment of the present invention.
Figure 3 shows a configuration diagram for a dental membrane filter according to an embodiment of the present invention.
Figure 4 shows a configuration diagram for a dental membrane filter according to an embodiment of the present invention.
5 is a conceptual diagram illustrating a method of forming a shielding layer having spikes through rolling molding according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein.

[Production Example 1: Production of Membrane Filter]

A shielding layer having fine spikes formed on both sides was fabricated through rolling molding by heating and pressing. The shielding layer was molded by mixing collagen and PGA. On the other hand, the shielding layer was laminated between the support layer and the polymer film layer having density properties as shown in [Table 1] below, and pressurized to prepare a filter. Then, it was observed whether the spikes formed on the shielding layer collapsed according to the density of the support layer and the polymer film. Meanwhile, the support layer and the polymer film layer were made of PG, and exhibited the same physical properties except for density.

M1 M2 M3 M4 M5 M6 g/cm ³ 0.4 0.6 0.8 1.1 1.3 1.6

[Experimental Example 1: Spike and Flexibility Evaluation]

The level of spike retention (S) of the support layer was observed in M1 to M6. When the spike structure of the support layer was maintained without being buried in the support layer and the polymer film layer, it was marked as O, and when the spike structure was buried and collapsed, it was evaluated as X. In addition, the flexibility (U) of the entire fabricated membrane filter was evaluated, and force was applied to the membrane filter to evaluate the flexibility, while maintaining the spike structure and usability as a membrane filter. O, otherwise, X was evaluated. The results are shown in [Table 2] below.

M1 M2 M3 M4 M5 M6 S X O O O O O U O O O O O X

As can be seen in [Table 2], in the case of M1, the spikes of the shielding layer were buried in the support layer and the polymer film layer, and in the case of M6, it could not be used as a membrane filter due to its hardness. Therefore, it can be seen that in the case of M2 to M5, it is possible to manufacture a membrane filter having a certain degree of flexibility while maintaining the spike structure of the shielding layer.

In the case of the spiked shielding layer, not only can the shielding effect be shown for a certain period of time, but also it can be used as a more effective membrane filter by reducing the content of the biodegradable material so that rapid biodegradation can proceed at the necessary time. can

[Preparation Example 2: Preparation of natural extract]

1. Preparation of Achinacea extract (AE)

After washing and drying the achinacea, it was pulverized and extracted three times with ethanol as a solvent. Thereafter, it was concentrated under reduced pressure to prepare an extract AE.

2. Preparation of other extracts

Acai berry extract (BE) in the same manner as in the AE; Arborvitae extract (CE); A sage extract (DE) and an extract (EE) were prepared.

3. Preparation of mixed extract

In order to evaluate the synergistic activity according to the action of each active ingredient by mixing each extract, a mixed extract was prepared by mixing the AE to EE with the composition shown in [Table 3] below.

T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 AE 100 100 100 100 100 100 100 100 100 100 BE 10 20 30 40 50 30 30 30 30 30 CE 5 10 15 20 25 15 15 15 15 15 DE - - - - - 2 5 7 10 15 EE - - - - - 5 10 15 20 30

(unit: parts by weight)

[Experimental Example 2: Evaluation of anti-inflammatory activity]

1. Evaluation of antibacterial activity against oral microorganisms

The evaluation results for the antibacterial activity against oral microorganisms for the AEs and T1 to T10 were evaluated. Oral microorganisms were used by culturing Streptococcus mutans (S. mutans). For objective comparison, Con treated with purified water was fixed as 1, and each Example was evaluated on a scale of 1 to 10. The higher the number, the better the antibacterial activity against oral microorganisms. The results are shown in [Table 4] below.

Con AE T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 A/B One 4.5 4 6.5 6 6.5 4.5 6 8.5 8.5 8 6.5

(Unit: index)

Referring to [Table 4], it can be confirmed that antibacterial activity against oral microorganisms is exhibited in the case of AE. In addition, when mixing in the range of T2 to T4, a synergistic effect due to the interaction of each composition can be confirmed. Through this, it can be confirmed that excellent antibacterial activity can be exhibited with a smaller content. And it can be seen that in the case of the range of T7 to T9, an additional synergistic effect is exhibited. This can be understood as the difference in the mechanism of the composition forming the mixing and the synergistic effect caused by each mixing occurring within a certain mixing range.

Through this, it is possible to effectively suppress the growth of microorganisms in the oral cavity without side effects such as cytotoxicity at a lower content than in the case of the composition, and increase the effect of removing the inflammatory action caused by the microorganisms in the oral cavity.

2. Assessment of pro-inflammatory mediator production inhibitory activity

Anti-inflammatory activity of each composition was evaluated by evaluating the production of pro-inflammatory mediators in the MC3T3-E1 osteoblast model in which an inflammatory state was induced by LPS (Lipopolysaccharides) while treating AE and T1 to T10. NO (Nitric oxide) was detected using a commercially available NO (Nitric oxide) analysis kit, and the absorbance was measured at 520 nm. In addition, the concentration of prostaglandin E2 (PGE ₂ ) was measured at 440 nm absorbance using an ELISA reader using a PGE ₂ ELISA kit.

Meanwhile, purified water was used as a control group. In addition, for objective comparison, the case of using purified water was fixed as an index of 1, and the case of AE and T1 to T10 treatment was comparatively evaluated with an index of 1 to 10. The higher the index, the better the anti-inflammatory activity due to the inhibitory activity of pro-inflammatory mediators. The results are shown in [Table 5] below.

Con AE T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 NO generation One 5 5 7 7 7.5 4.5 6.5 8.5 8 8 6 Generate PGE ₂ One 3 3.5 6 5.5 6 3 5 7 7.5 7.5 5.5

(Unit: Index)

Referring to [Table 5], it can be confirmed that the effect of inhibiting the production of pro-inflammatory mediators appears in the case of AE. In addition, it can be seen that in the case of T2 to T4, a synergistic effect due to the interaction of the active ingredients included in each composition appears, and additional synergistic activity is observed in the range of T7 to T9.

3. Assessment of inhibitory activity against the production of pro-inflammatory cytokines

Evaluation of pro-inflammatory cytokine production in the MC3T3-E1 osteoblast model in which inflammation was induced by LPS (Lipopolysaccharides) while processing AE and T1 to T10 by protein extraction and Western blot analysis did Purified water was used as a control group, and for objective evaluation, the degree of production of pro-inflammatory cytokines was observed and evaluated on a scale of 1 to 10. The higher the index, the better the cytokine production inhibitory effect.

The results are shown in [Table 6] below.

Con AE T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 A/B One 5.5 5 7 7 7.5 4.5 6.5 9 9 8.5 6

(Unit: Index)

Referring to [Table 6], it was confirmed that the mRNA and protein expression inhibitory activity of pro-inflammatory cytokines appeared in AE. In addition, it was confirmed that through the synergistic effect from T2 to T4 and the additional synergistic effect from T7 to T9, the inhibitory activity and significant effect on the production of previous pro-inflammatory mediators appeared.

Through this, it can be seen that in the case of the composition, it is possible to effectively suppress or alleviate inflammatory diseases in the oral cavity caused by the implant procedure. In addition, in the case of using the mixed composition within a certain range, it is possible to show a better effect without side effects such as cytotoxicity with a smaller content through synergistic activity due to the interaction of each active ingredient.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also made according to the present invention. falls within the scope of the rights of

100: support layer
200: shielding layer
210: Spike
300: polymer film layer
1000: dental membrane filter

Claims (8)

A support layer containing a biodegradable polymer;
a shielding layer laminated on an upper surface of the support layer and containing collagen and a biodegradable polymer; and
Including a polymer film laminated on the upper surface of the shielding layer,
The shielding layer is formed with spikes on one or both sides
Dental membrane filter. According to claim 1,
The biodegradable polymer is polyglycolide (PG), polyglycolic acid (PGA), polylactic acid (PLA), polylactic acid (PLLA), poly-D,L-lactic acid (PDLLA), polycaprolactone (PCL), Any one selected from polydioxanone (PDO) and mixtures thereof
Dental membrane filter. According to claim 2,
The polymer film is thinner than the shielding layer
Dental membrane filter. According to claim 3,
the support layer; the shielding layer; And the polymer film is formed with pores
Dental membrane filter. delete According to claim 1,
The polymer film further comprises chitosan and a hydrophilic additive
Dental membrane filter. Laminating a shielding layer containing collagen and a biodegradable polymer on an upper surface of a support layer made of a biodegradable polymer; and
Comprising the step of laminating a polymer film on the upper surface of the shielding layer
Manufacturing method of dental membrane filter. Forming spikes on one or more surfaces of the shielding layer using any one of roller molding, mold molding, or plasma treatment on a shielding layer containing collagen and a biodegradable polymer
Method for manufacturing a shielding layer for a dental membrane filter.

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