KR102501448B1 - Plate for cells freezing and thawing - Google Patents

Abstract

The present invention vertically protrudes along the periphery of the bottom part to form a body with an open upper side, and a plurality of protruding vertically at regular intervals from the bottom surface of the space of the body, and cells and cells on the upper surface. A cell accommodating part accommodating a heat medium solution, and a cover coupled to the upper side of the body to seal the space of the body, wherein the accommodating space of any one cell accommodating part among the plurality of cell accommodating parts is the rest of the cell accommodating part. By forming a larger capacity than the accommodating space, the capacity of some of the cell accommodating units of the plurality of cell accommodating units constituting the array is larger than the capacity of the other cell accommodating units, and the plurality of cell accommodating units constituting the array are numbered in order, A plate for freezing and thawing cells that can meet the various needs of users (researchers) in the process of freezing and thawing with one plate by making it usable by giving various changes to cell freezing and thawing according to the user's purpose and method. to provide.

Description

Plate for cell freezing and thawing {Plate for cells freezing and thawing}

The present invention relates to a plate for freezing and thawing cells, and more particularly, allows cells such as eggs and embryos to be used in various ways according to the user's purpose and method, so that a single plate can be used in the process of freezing and thawing. It relates to a plate for freezing and thawing cells that can meet the various needs of users (researchers).

In general, criteria for automated sample acceptance have been established in many parts of the pharmaceutical, medical and biotechnology sectors, and this has mainly occurred during the development of high-throughput screening (so-called SBS standards).

Here, what is used in laboratory practice is a multiwell substrate with a very small round structure that forms reaction spaces, also known as wells, cavities, and cups, arranged in rows and columns, such as samples, e.g. Eggs, embryos, cell material, blood samples, etc. are introduced.

These are flat plastic multiwell substrates with 6, 8, 16, 24 to 96 or more individual wells. This type has been standardized throughout the world and is used in pipetting machines, cell cultivators, and also used in diagnostic as well as analytical technology device platforms.

In addition to this, freezing and thawing protocols are defined. By cryopreservation, the suspension located in the well substrate is removed and transferred from individual tubes, straws, individual plastic containers, etc.

For example, after receiving a sample in each well of a well plate having a plurality of wells having the same capacity, receiving a heating medium solution together with all the received samples, freezing or thawing the sample only in a predetermined manner under the same conditions. going through the process.

Here, in the conventional well plate, since the capacity of the wells for accommodating samples is uniform, various changes in cell freezing and thawing cannot be made according to the user's purpose and method.

As for the prior art, reference may be made to Patent Registration No. 10-2103237 (2020.04.16).

In the present invention, the capacity of some of the cell accommodating units among a plurality of cell accommodating units disposed in a row on the body is larger than the capacity of the other cell accommodating units, and then cells are stored in each cell accommodating unit according to the user's purpose and method. Its purpose is to provide a plate for freezing and thawing cells that can meet the various needs of users (researchers) in the process of freezing and thawing with a single plate by storing a heating medium solution necessary for freezing and thawing.
In addition, in the present invention, after forming a plurality of cell accommodating units arranged in a row on the body with different capacities, each cell accommodating unit stores a heat medium solution necessary for cell freezing and thawing according to the user's purpose and method. Its purpose is to provide a plate for freezing and thawing cells that can meet the various needs of users (researchers) in the process of freezing and thawing with one plate.

In addition, according to the present invention, by sequentially numbering a plurality of cell accommodating units in which different capacities of heating medium solutions are stored, the cell accommodating unit giving various changes to cell freezing and thawing according to the user's purpose and method is easily recognized. Its object is to provide a plate for freezing and thawing of cells capable of.

The plate for freezing and thawing cells according to the present invention includes a body having a plurality of cell accommodating units accommodating cells and a heat medium solution, respectively, on the upper surface, and a plurality of cell accommodating portions formed on the upper surface of the body coupled to the upper surface of the body. A cover is included to seal the unit, and an accommodation space of any one cell accommodation unit among the plurality of cell accommodation units has a larger capacity than the accommodation space of the other cell accommodation units.

At this time, four cell receiving units according to the present invention form one line in a row, and form two lines on the body.

Here, in the plate for freezing and thawing cells according to the present invention, the capacity of the cell accommodating part located on the left side of the four cell accommodating parts constituting one row is relatively larger than the capacity of the remaining three cell accommodating parts, and the remaining It is preferable that the capacities of the three cell accommodating unit accommodating spaces are formed with the same capacity.

In addition, in the plate for freezing and thawing cells according to the present invention, the four cell accommodating units constituting one row may sequentially form a smaller accommodating space capacity of the cell accommodating unit from left to right.

In addition, one of the two streams according to the present invention forms a first display unit in which numbers are marked in order from the cell accommodating unit with a large capacity, and the other sequence displays numbers in order from the cell accommodating unit with a small capacity. It is preferable to form the marked second display portion.

In addition, the body according to the present invention may form a space with an open upper side by vertically protruding the rim along the periphery of the bottom portion.

At this time, the cell accommodating part according to the present invention may be formed in a columnar shape in the space of the body, and a hemispherical concave accommodating space may be formed on the upper surface to accommodate the cells and the heat medium solution.

In addition, a flange portion protrudes outward from the outer circumferential surface of the rim of the body according to the present invention, and a plurality of grip holders that the user grips with fingers are formed on the flange portion.

In addition, the cover according to the present invention forms a plurality of holders for holding frozen straws, respectively, on its upper surface, and the plurality of holders form a third display unit displaying numbers in order from one side to the other, stacked on the upper surface Spaced protrusions are formed to space the body to a corresponding height.

Effects exhibited by the plate for freezing and thawing cells according to the present invention are as follows.

Among the plurality of cell accommodating units arranged in a row on the body, the capacity of some of the cell accommodating units is larger than that of the rest of the cell accommodating units, and then each cell accommodating unit is required for freezing and thawing of cells according to the user's purpose and method. It has the effect of being able to meet the various needs of users (researchers) in the process of freezing and thawing with one plate by storing the heating medium solution.

In addition, after forming a plurality of cell accommodating units arranged in a row on the body with different capacities, a heat medium solution necessary for freezing and thawing cells is stored in each cell accommodating unit according to the user's purpose and method, It has the effect of meeting the various needs of users (researchers) in the process of freezing and thawing as a plate.
In addition, by sequentially numbering a plurality of cell accommodating units in which heat medium solutions of different capacities are stored, various changes in cell freezing and thawing can be easily recognized according to the user's purpose and method. It works.

1 is an exemplary view showing a plate for freezing and thawing cells according to an embodiment of the present invention.
2 is a plan view of a cover and a body of a plate for freezing and thawing cells according to an embodiment of the present invention.
3 is an exemplary view showing a state in which a freezing straw is applied to a plate for freezing and thawing cells according to an embodiment of the present invention.
4 is an exemplary diagram showing a state in which a plurality of plates for freezing and thawing cells are stacked on each other according to an embodiment of the present invention.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. Prior to this, the terms or words used in this specification and claims should not be construed as being limited to the usual or dictionary meaning, and the inventor appropriately uses the concept of the term in order to explain his/her invention in the best way. Based on the principle that it can be defined, it should be interpreted as meaning and concept consistent with the technical spirit of the present invention.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all the technical ideas of the present invention, so at the time of this application, they are equivalent alternatives that can be replaced. It should be understood that variations may exist.

The present invention allows cells such as eggs and embryos to be used with various changes according to the user's purpose and method, so that in the process of freezing and thawing with one plate, cell freezing and thawing that can meet the various needs of users (researchers) Regarding the plate for use, the following will be considered in more detail with reference to the drawings.

The plate for freezing and thawing cells according to an embodiment of the present invention with reference to FIGS. 1 to 4 includes a body 100 and a cover 200. First, the body 100 has plasticity by heat, It is preferable to be made of poly-styrene (PS) material, which has excellent chemical resistance and is transparent and easy to observe under a microscope. A cell receiving unit 110 is formed.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, an edge 101 of a certain height is protruded vertically along the periphery of the bottom portion, so that the inside of the body 100 is a rectangular parallelepiped with an open upper side. It is preferable to form a space 102 of the shape, and form a flange portion 103 having a corresponding thickness to extend outward on the lower side of the outer surface of the rim 101.

At this time, a plurality of grip holders 104 that the user grips with fingers are formed on the flange portion 103, and the grip holders 104 are a pair of two so that the user's thumb and index finger can each be gripped. It is formed on the left and right sides based on the center line dividing the center of 100, so that the user can handle the body 100 by gripping it with the thumb and forefinger of both hands, respectively.

Here, when cells such as eggs and embryos are frozen and thawed, all processes must be performed within the correct time. When holding a normal plate in the hand, users (researchers) put a lot of force on their fingers, resulting in increased fatigue.

However, in the body 100 according to an embodiment of the present invention, a flange portion 103 protrudes outward from the outer circumferential surface of the rim 101, and a plurality of grips that the user grips with fingers are formed on the flange portion 103. As the holder 104 is formed, in the process of freezing or thawing the cells, the cover 200 is separated from the body 100 and put aside, and the plate is held according to the position of the cell accommodation unit 110 in which the cells are accommodated. Because the position is different, you can safely hold and operate the plate.

In addition, by rounding the end of the grip holder 104, injury that may occur when the user grips the plate with his or her fingers is prevented in advance.

In addition, a plurality of cell accommodating units 110 are formed inside the space 102 of the body 100, and the cell accommodating units 110 accommodate cells and a heat medium solution required for freezing and thawing. Four cell receiving units 110 are arranged in a row along the longitudinal direction of the body 100 to form one row, and inside the space 102 of the body 100, the two rows are formed. it is desirable

Therefore, a total of eight cell receiving units 110 are formed in the body 100 according to an embodiment of the present invention.

Also, the capacity of the accommodating space 111 of the cell accommodating unit 110 located on the left side of the four cell accommodating units 110 forming one row is relatively higher than the capacity of the accommodating space 111 of the remaining three cell accommodating units 110. , and the capacities of the remaining three cell accommodating units 110 and accommodating spaces 111 are formed with the same capacity.

For example, among the four cell accommodating units 110 forming one row, the cell accommodating unit 110 located on the left side relative to the length of the body 100 has a hemispherical accommodating space 111 with a diameter of 14.7 mm. It is formed to accommodate the cells and the heat medium solution with a capacity of 0.36cc, and the remaining three cell accommodating parts 110 have a hemispherical accommodating space 111 with a diameter of 11.6mm to form a 0.23cc It is preferable to form a capacity to accommodate the cells and the heat medium solution.

Therefore, by forming the cell accommodation unit 110 smaller than the conventional type of freezing and thawing plate, the volume of the heating medium solution used for freezing or thawing is reduced to seek cost reduction.

In addition, when freezing and thawing cells, the amount of heat medium solution used varies depending on the user's request or the type of cells to be frozen and thawed. One cell accommodating unit 110 having a relatively large capacity of the accommodating space 111 and three cell accommodating units 110 having a relatively small capacity of the accommodating space 111 form one line, and one Among the cell accommodating units 110 forming a row, the cell accommodating unit 110 having a large capacity of the accommodating space 111 is disposed on the outermost left side, forming the first display unit 112 or the second display unit 113. . The first display unit 112 and the second display unit 113 are marked with Arabic numerals. In the first display unit 112, any one of the two rows displays numbers in order from the cell receiving unit with the largest capacity And, in the second display unit 113, the other row displays numbers in order from the cell receiving unit with the smallest capacity.

Therefore, among the cell accommodating units 110 forming one row, the cell accommodating unit 110 having a large capacity of the accommodating space 111 is marked with the number 1 from the outermost left, and the remaining three are numbered 2 to 1 in order. Numbers up to number 4 are indicated, and cell accommodation units 110 in other rows are marked with numbers 1 to 4 in order from the right side, on the contrary, the cell accommodation unit 110 having a large capacity of the accommodation space 111 ), and two cases in which the capacity of the accommodating space 111 can be used from the cell accommodating unit 110 with a small capacity are provided.

For example, when cells such as eggs and embryos are frozen, the method of first washing these cells in the basic culture medium and then transferring them to the frozen culture medium requires 300ul culture medium. After that, the 200ul culture medium is transferred to the frozen culture medium of the cell receiving unit 110 having a small capacity to proceed with the cell freezing procedure. Freezing is performed by sequentially using the cell accommodating units 110 from the direction of the unit 110.

If cell freezing is performed directly in the 200ul frozen culture medium of the cell accommodating unit 110 with a small capacity without washing in the basic culture medium, the small cells of the row of cell accommodating unit 110 having a small capacity among the two arrays are No. 1 Freezing is performed by sequentially using the cell accommodating units 110 from the direction of the accommodating unit 110 .

In addition, when thawing cells such as eggs and embryos, if they are frozen with a freezing straw, open the lid of the freezing straw and immerse the tip of the frozen straw containing the cells such as eggs and embryos directly into the culture medium (thawing solution). In case of use, since it must be performed first in the thawed culture medium of the cell accommodating unit 110 having a large capacity into which 300ul culture medium is entered, the large cell accommodating unit 110 of the first row of the cell accommodating unit 110 having a large capacity among the two rows Thawing is performed by sequentially using the cell accommodating units 110 from the same direction.

If the culture solution (thawing solution) is put in a micropipette in advance, the thawing solution and the freezing solution are mixed little by little, then sucked up into the micropipette and then transferred to the thawing solution, the cell receiving unit with a small capacity of 200ul of the culture solution ( 110), since the thawing is performed in the thawing culture medium, the cell accommodating part 110 with the smallest capacity among the two rows is sequentially used from the direction of the small cell accommodating part 110 of the row No. 1 to perform thawing. Start.

Therefore, as shown in the above example, since it can be used with various changes depending on the purpose and method of freezing and thawing by a user, it is advantageous that it can be tailored to the various needs of users during the freezing and thawing process.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, the four cell accommodating units 110 constituting one row increase the capacity of the cell accommodating unit 110 sequentially from left to right. It can also be made small.

At this time, it is preferable to form the capacity of the receiving space of the cell accommodating unit 110 sequentially smaller than a certain ratio.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, an edge 101 of a certain height is vertically protruded along the periphery of the bottom of the body 100, so that the inside of the body 100 When a rectangular parallelepiped-shaped space 102 with an open upper side is formed, a plurality of cells protrude vertically at regular intervals from the bottom surface of the space 102 of the body 100, and cells and a heat medium solution are accommodated on the upper surface.

More specifically, the cell accommodating part 110 has a columnar shape, and forms a hemispherical concave accommodating space 111 on the upper surface to accommodate the cells and the heat medium solution.

At this time, four of the plurality of cell accommodating units 110 are arranged in a line along the longitudinal direction of the body 100 to form a single row, and the above-mentioned two rows are inside the space 102 of the body 100. It is preferable to form

Therefore, a total of 8 cell accommodating units 110 are formed inside the space of the body 100 according to an embodiment of the present invention.

In addition, the cover 200 is coupled to the upper side of the body 100 according to an embodiment of the present invention. The cover 200 is coupled to the upper side of the body 100, and the space of the body 100 ( 102) is sealed.

At this time, the cover 200 is preferably formed with an area including the space 102 and the rim 101 of the body 100, so as to seal the space 102 of the body 100, A cover edge 201 extending downward is formed around the cover 200 .

Therefore, when the cover 200 is coupled to the upper side of the body 100, the inner surface of the cover edge 201 and the outer surface of the edge 101 of the body 100 are in close contact with each other, so that the body 100 The space 102 of ) is sealed.

In addition, a plurality of holding parts 210 are formed on the upper surface of the cover 200, and the plurality of holding parts 210 hold a freezing straw or the like.

At this time, the holder 210 is preferably formed as a groove corresponding to the width of the frozen straw on a protrusion protruding vertically from the left and right sides or front and rear sides of the upper surface of the cover 200, and the groove is It is preferable to form a plurality of cells corresponding to the number of cell accommodating units 110 formed in the space 102 of the body 100, and the plurality of grooves also correspond to the cell accommodating units 110 in the third display unit. To form, the third display unit also displays numbers in order from one side to the other side of the plurality of holders 210.

Therefore, when fertilized eggs or oocytes are frozen, each patient prepares the procedure by putting the culture medium in a plate. When there are many patients who need to freeze, each freezing straw is manufactured and placed on each plate so that the frozen straws prepared for each patient do not mix. Thus, the frozen straws to be placed are mixed or the user can intuitively recognize the cell accommodating unit 110 and the corresponding frozen straw without confusion.

In addition, the cover 200 forms a separation protrusion 220 on the upper surface, and the separation protrusion 220 separates the body 100 stacked on the upper surface of the cover 200 to a corresponding height.

In general, when packaging or storing plates for freezing and thawing cells, several plates are stacked. When the plates are stacked and stacked, a gap of 0.3 mm is created between the lower cover and the upper body, which prevents the flow of fluid between layers during storage. make it easy

Although the present invention has been described with reference to the embodiments shown in the drawings, this is merely exemplary, and those skilled in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

100: body
101: border
102: space
103: flange part
104: grip holder
110: cell reception unit
111: accommodation space
112: first display unit
113: second display unit
200: cover
201: cover border
210: mounting part
220: spaced protrusion

Claims (9)

A plurality of cell accommodating units accommodating cells and a heat medium solution are formed on the upper surface, and four cell accommodating units form a row, and the cells are located on the left side of the four cell accommodating units forming a row. a body in which the capacity of the accommodating space of the accommodating unit is relatively greater than that of the remaining three cell accommodating units, and the capacity of the accommodating space of the remaining three cell accommodating units is the same; and
A cover coupled to the upper side of the body to seal the plurality of cell receiving units formed on the upper surface of the body;
The body forms two rows of four cell receiving units in a row,
An accommodating space of any one of the plurality of cell accommodating units forms a larger capacity than the accommodating space of the other cell accommodating units,
the cover,
Forming a plurality of holding portions for holding frozen straws on the upper surface, respectively,
The plurality of holders form a third display unit in which numbers are marked in order from one side to the other side,
A plate for freezing and thawing cells formed with spaced protrusions that separate the bodies to be stacked on the upper surface at the corresponding height.

delete delete A plurality of cell accommodating units for accommodating cells and a heat medium solution are formed on the upper surface, but four cell accommodating units form a line in a row, and the four cell accommodating units constituting a sequence are moving from left to right. a body in which the capacity of the cell accommodating unit is sequentially reduced; and
A cover coupled to the upper side of the body to seal the plurality of cell receiving units formed on the upper surface of the body;
The body forms two rows of four cell receiving units in a row,
An accommodating space of any one of the plurality of cell accommodating units forms a larger capacity than the accommodating space of the other cell accommodating units,
the cover,
Forming a plurality of holding portions for holding frozen straws on the upper surface, respectively,
The plurality of holders form a third display unit in which numbers are marked in order from one side to the other side,
A plate for freezing and thawing cells formed with spaced protrusions that separate the bodies to be stacked on the upper surface at the corresponding height.
According to claim 1 or claim 4,
One of the two rows forms a first display section with numbers marked in order from the cell receiving section with the largest capacity,
The other row is a plate for freezing and thawing cells forming a second display section with numbers marked in order from the cell reception section with a small capacity.
According to claim 1 or claim 4,
the body
A plate for freezing and thawing cells in which a rim is vertically protruded along the periphery of the bottom part to form a space with an open top.
According to claim 1 or claim 4,
The cell receptor
A plate for freezing and thawing cells formed in a columnar shape in the space of the body and having a hemispherical concave accommodation space formed on the upper surface to accommodate cells and a heat medium solution.
According to claim 1 or claim 4,
Forming a flange portion extending outward from the outer circumferential surface of the rim of the body,
A plate for freezing and thawing cells in which a plurality of grip holders gripped by a user with fingers are formed on the flange portion.
delete

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