KR20220059716A - Plate for cells freezing and thawing - Google Patents

Abstract

The present invention provides a plate for freezing and thawing cells. The plate includes: a body in which a rim is vertically protruded along the periphery of a bottom part to form a space with an open upper side; a plurality of cell accommodating parts protruding vertically at regular intervals from the bottom surface of the space of the body, and accommodating cells and heat medium solutions on the upper surface; and a cover coupled to the upper side of the body to seal the space of the body. An accommodating space of any one of the plurality of cell accommodating parts forms a larger capacity than accommodating spaces of the other cell accommodating parts. Among a plurality of cell receptors forming a row, the capacity of some of the cell receptors is larger than that of the rest of the cell receptors, and the plurality of cell receptors forming a row are sequentially numbered. Usage can be ensured while giving various changes to cell freezing and thawing according to a user's purpose and method, and thus one plate can meet the various needs of users (researchers) in a process of freezing and thawing.

Description

Plate for cells freezing and thawing

The present invention relates to a plate for freezing and thawing cells, and more particularly, it allows cells such as eggs and embryos to be used with various changes according to the purpose and method of the user, so that in the process of freezing and thawing with one plate, It relates to a plate for freezing and thawing cells that can meet the various needs of users (researchers).

In general, in many areas of the pharmaceutical, medical and biotechnology fields, automated sample acceptance criteria have been established, and this mainly occurred during the development of high-throughput screening (the so-called SBS standard).

Here, used in laboratory practice is a multi-well substrate with a very small, round structure that forms a reaction space, also known as a well, a cavity, or a cup, which is arranged in rows and columns to store samples, e.g. Eggs, embryos, cellular material, blood samples, etc. are introduced.

These are planar plastic multiwell substrates with 6, 8, 16, 24 to 96 or more individual wells. This form has been standardized throughout the world, and is used in pipetting machines and cell culture machines, and is also used in device platforms for diagnostic as well as analysis technology.

As well as this defined as a freezing and thawing protocol, by cryopreservation, suspensions located within the well substrates, such as individual tubes, straws, and individual plastic containers, are removed and transported.

For example, after accommodating a sample in each well of a well plate having a plurality of wells having the same capacity, receiving a thermal medium solution together with all the received samples, freezing or thawing the sample only in a predetermined manner under the same conditions will proceed with the process.

Here, in the conventional well plate, the capacity of the well for receiving the sample is uniformly equal, so it is impossible to give various changes to freezing and thawing of the cells according to the purpose and method of the user.

As a prior art, reference may be made to Korean Patent Registration No. 10-2103237 (2020.04.16).

In the present invention, the capacity of some cell receptors among a plurality of cell receptors forming the rank is greater than the capacity of the other cell receptors, and numbering the plurality of cell receptors forming the rank sequentially, depending on the purpose and method of the user. The purpose of this is to provide a plate for freezing and thawing cells that can be used by giving various changes to cell freezing and thawing according to the needs of users (researchers) in the freezing and thawing process with one plate. do.

The plate for freezing and thawing cells according to the present invention includes a body having a plurality of cell receiving units accommodating cells and a heating medium solution respectively on the upper surface, and a plurality of cell receiving units formed on the upper surface of the body by being coupled to the upper side of the body and a cover sealing the part, wherein the accommodating space of any one of the plurality of cell accommodating units has a larger capacity than the accommodating space of the other cell accommodating units.

At this time, four cell receptors according to the present invention form one line in a line, and two lines are formed in the body.

Here, in the plate for freezing and thawing cells according to the present invention, the receiving space capacity of the cell receiving unit located on the left among the four cell receiving units forming one line is relatively larger than the receiving space capacity of the remaining three cell receiving units, and the remaining It is preferable to form the three cell receptor receiving space capacity to have the same capacity.

In addition, in the plate for freezing and thawing cells according to the present invention, the four cell receptors constituting one line may sequentially decrease the capacity of the cell receptor receiving space from left to right.

And, any one of the two sequences according to the present invention forms a first display part in which numbers are marked in order from the cell receptor with a large capacity, and the other sequence shows the numbers in order from the cell receptor with a small capacity. It is preferable to form the marked second display unit.

In addition, the body according to the present invention may form a vertically protruding edge along the periphery of the bottom to form a space with an open upper side.

At this time, the cell accommodating part according to the present invention may be formed in a columnar shape in the space of the body, and a hemispherical concave accommodating space may be formed on the upper surface to accommodate the cells and the heating medium solution.

And a flange portion is formed to protrude outward from the outer peripheral surface of the rim of the body according to the present invention, and a plurality of grip holders to be gripped by the user with fingers are formed on the flange portion.

In addition, the cover according to the present invention forms a plurality of cradles for holding the frozen straw on its upper surface, and the plurality of cradles forms a third display part marked with numbers in order from one side to the other, and is laminated on the upper surface A spaced protrusion is formed to separate the body to the corresponding height.

The effects exhibited by the plate for freezing and thawing cells according to the present invention are as follows.

Cells are frozen according to the purpose and method of the user by sequentially numbering the plurality of cell receptors in the rank while forming the cell receptor capacity of some of the plurality of cell receptors in the rank larger than the capacity of the other cell receptors. And by giving various changes for thawing, it has the effect of being able to meet the various needs of users (researchers) in the process of freezing and thawing with one plate.

1 is an exemplary view showing a plate for freezing and thawing cells according to an embodiment of the present invention.
2 is a plan view of a cover and a body of a plate for freezing and thawing cells according to an embodiment of the present invention.
3 is an exemplary view showing a state in which a freezing straw is mounted on a plate for freezing and thawing cells according to an embodiment of the present invention.
4 is an exemplary view showing a state in which a plurality of plates for freezing and thawing cells are stacked on each other according to an embodiment of the present invention.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. Prior to this, the terms or words used in the present specification and claims should not be construed as being limited to conventional or dictionary meanings, and the inventor should properly understand the concept of the term in order to best describe his invention. Based on the principle that can be defined, it should be interpreted as meaning and concept consistent with the technical idea of the present invention.

Accordingly, the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all of the technical spirit of the present invention. It should be understood that there may be variations.

The present invention allows cells such as eggs and embryos to be used with various changes according to the purpose and method of the user, so that the freezing and thawing process with one plate can meet the various needs of users (researchers). It relates to a plate for use, and will be described in more detail with reference to the drawings.

The plate for freezing and thawing cells according to an embodiment of the present invention with reference to FIGS. 1 to 4 includes a body 100 and a cover 200. First, the body 100 has plasticity due to heat, It is preferably made of a poly-styrene (PS) material that has excellent chemical resistance and is transparent so that it is easy to observe under a microscope. The cell receptor 110 is formed.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, a rim 101 of a certain height is formed to protrude vertically along the periphery of the bottom part, and the body 100 is a rectangular parallelepiped with an open upper side inside the body 100 . It is preferable to form a space 102 of the shape, and to extend outwardly the flange portion 103 having the corresponding thickness on the lower side of the outer surface of the edge 101 .

At this time, a plurality of grip holders 104 to be gripped by the user's fingers are formed on the flange portion 103, and two grip holders 104 are provided as a pair so that the user's thumb and index finger can be gripped respectively. It is formed on the left and right sides based on the center line dividing the center of 100 , so that the user can grip and handle the body 100 with the thumb and index finger of both hands.

Here, when freezing and thawing cells such as eggs and embryos, all processes must be performed within the correct time period.

However, in the body 100 according to an embodiment of the present invention, the flange portion 103 protrudes outwardly from the outer circumferential surface of the rim 101, and the flange portion 103 has a plurality of grips gripped by the user with fingers. As the holder 104 is formed, in the process of freezing or thawing the cells, remove the cover 200 from the body 100 and set it aside, and hold the plate according to the position of the cell receiving unit 110 in which the cells are accommodated. Because the position changes, the plate can be safely grasped and manipulated.

In addition, by rounding the end of the grip holder 104, an injury that may occur when the user grips the plate with his or her fingers is prevented in advance.

And a plurality of cell receiving units 110 are formed inside the space 102 of the body 100, and the cell receiving unit 110 accommodates cells and a heating medium solution required for freezing and thawing, in this case, the plurality of Four of the cell receiving units 110 are arranged in a line along the longitudinal direction of the body 100 to form one line, and the two cell receiving units 110 are formed in the space 102 of the body 100 as described above. it is preferable

Therefore, a total of eight cell receiving units 110 are formed in the body 100 according to an embodiment of the present invention.

And the capacity of the receiving space 111 of the cell receiving unit 110 located on the left among the four cell receiving units 110 constituting one row is relative to the capacity of the remaining three cell receiving units 110 receiving space 111 . , and the remaining three cell accommodating units 110 and accommodating spaces 111 have the same capacity.

For example, among the four cell receiving units 110 forming one line, the cell receiving unit 110 located on the left based on the length of the body 100 has a hemispherical receiving space 111 with a diameter of 14.7 mm. to accommodate the cell and thermal medium solution with a capacity of 0.36 cc, and the remaining three cell accommodating units 110 have a hemispherical accommodating space 111 with a diameter of 11.6 mm, with a diameter of 0.23 cc. It is preferable to form so as to accommodate the cell and thermal medium solution in a capacity.

Therefore, by forming the cell receiving unit 110 smaller than the conventional type of freezing and thawing plate, the capacity of the thermal medium solution used for freezing or thawing is reduced to reduce costs.

In addition, when freezing and thawing cells, the amount of thermal medium solution used varies depending on the user's needs or the type of cells to be frozen and thawed. One cell receiving unit 110 having a relatively large capacity of the receiving space 111 and three cell receiving units 110 having a relatively small capacity of the receiving space 111 form a line, and one A first display unit 112 or a second display unit 113 is formed while arranging the cell receiving unit 110 having a large capacity of the receiving space 111 among the cell receiving units 110 in the line at the outermost left side. . The first display unit 112 and the second display unit 113 and are marked with Arabic numerals, and the first display unit 112 displays numbers in order from the cell receiving unit with the largest capacity in any one of the two lines. and, the second display unit 113 displays numbers in order from the cell receiving unit with the smallest capacity in the other row.

Therefore, among the cell receiving units 110 constituting one row, the cell receiving unit 110 having a large capacity of the receiving space 111 is denoted by number 1 from the outermost left, and the remaining three are numbered 2 in order. Numbers up to 4 are marked, and the cell receiving units 110 in other rows are marked with numbers 1 to 4 in order from the right side, so that the cell receiving units 110 having a large capacity of the receiving space 111 are marked. ), or a case in which the capacity of the accommodating space 111 can be used from the small cell accommodating unit 110 is provided.

For example, when freezing cells such as eggs and embryos, 300ul of culture medium is required in a method of first washing these cells in the basic culture medium and then transferring them to the freezing medium, and the first washing in the basic culture medium of the large cell receiving unit 110 After that, the cell freezing procedure is carried out by moving to the frozen culture medium of the cell receiving unit 110 with a small capacity of 200ul culture medium. Freezing is performed using the cell receiving units 110 sequentially from the direction of the unit 110 .

If, without washing in the basic culture medium, cells are frozen directly in 200ul of the cryoculture medium of the cell receiving unit 110 with a small capacity, the small cells in the 1st row of the cell receiving unit 110 with the smallest capacity among the two rows Freezing is performed using the cell receiving units 110 sequentially from the receiving unit 110 direction.

Also, when thawing cells such as eggs and embryos, if they are frozen with a frozen straw, open the lid of the frozen straw and immediately immerse the end of the frozen straw containing cells such as eggs and embryos in the culture solution (thaw solution). In the case of use, since the thawing culture medium of the cell receiving unit 110 with a large capacity of 300ul culture solution should be first performed, the cell receiving unit 110 with the largest capacity among the two lines is the largest cell receiving unit 110 in the row. The thawing is performed using the cell receptors 110 sequentially from the direction.

If the culture solution (thaw solution) is put in a micropipette in advance, mixed with the thawing solution and the freezing solution little by little, and then sucked up with a micropipette and then transferred to the thaw solution, the cell receiving part ( Since thawing is performed in the thawing culture solution of 110), thawing is performed by sequentially using the cell receiving units 110 from the direction of the small cell receiving unit 110 of the row in which the cell receiving unit 110 with the smallest capacity among the two rows is No. start

Therefore, as shown in the above example, it is advantageous in that it can be used with various changes depending on which user freezes and thaws for what purpose and by what method, so that it can meet the various needs of users during the freezing and thawing process.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, the four cell receiving units 110 forming a line sequentially increase the receiving space capacity of the cell receiving unit 110 from left to right. It can also be made small.

At this time, it is preferable that the capacity of the receiving space of the cell receiving unit 110 be sequentially smaller in comparison with a certain amount.

In addition, in the plate for freezing and thawing cells according to an embodiment of the present invention, a rim 101 of a predetermined height is formed to protrude vertically along the periphery of the bottom of the body 100, and the inside of the body 100 is When a rectangular parallelepiped-shaped space 102 with an open upper side is formed, a plurality of the space 102 of the body 100 vertically protrude at regular intervals from the bottom surface, and the cells and the heating medium solution are accommodated on the upper surface.

In more detail, the cell accommodating part 110 has a columnar shape, and a hemispherical concave accommodating space 111 is formed on the upper surface to accommodate the cells and the thermal medium solution.

At this time, four of the plurality of cell receiving units 110 are arranged in a line along the longitudinal direction of the body 100 to form a single line, and the two above-described lines are located inside the space 102 of the body 100 . It is preferable to form

Accordingly, a total of eight cell receiving units 110 are formed in the space of the body 100 according to an embodiment of the present invention.

And the cover 200 is coupled to the upper side of the body 100 according to an embodiment of the present invention, the cover 200 is coupled to the upper side of the body 100, the space of the body 100 ( 102) is sealed.

At this time, the cover 200 is preferably formed in an area including the space 102 and the edge 101 of the body 100, so as to seal the space 102 of the body 100, the A cover border 201 extending downwardly is formed around the cover 200 .

Therefore, when the cover 200 is coupled to the upper side of the body 100, the inner surface of the cover border 201 and the outer surface of the rim 101 of the body 100 are in close contact with each other to form the body 100 ) of the space 102 is sealed.

And a plurality of mounting portions 210 are formed on the upper surface of the cover 200, and the plurality of mounting portions 210 receive a frozen straw or the like.

At this time, the holding part 210 is preferably formed as a groove corresponding to the width of the frozen straw in the protrusion vertically protruding from the left or right sides or front and rear sides of the upper surface of the cover 200, and the groove is It is preferable to form a plurality of cells corresponding to the number of the cell receiving units 110 formed in the space 102 of the body 100 , and the plurality of grooves may also correspond to the cell receiving units 110 so as to correspond to the third display unit. In forming a , the third display unit also marks numbers in the order from one side to the other side among the plurality of mounting units 210 .

Therefore, when freezing a fertilized egg or egg, each patient prepares the procedure by putting the culture solution on a plate. Thus, it is possible to intuitively recognize the cell receiving unit 110 and the corresponding frozen straw without mixing or confusing the frozen straw to be placed.

In addition, the cover 200 forms a spaced protrusion 220 on the upper surface, and the spaced protrusion 220 separates the body 100 stacked on the upper surface of the cover 200 by a corresponding height.

In general, when packaging or storing plates for freezing and thawing cells, several plates are stacked. When stacking plates, a gap of 0.3 mm is created between the lower cover and the upper body, preventing the flow of fluid between the layers during storage. make it easy

Although the present invention has been described with reference to the embodiment shown in the drawings, which is only exemplary, those of ordinary skill in the art will understand that various modifications and equivalent other embodiments are possible therefrom. Therefore, the true technical protection scope of the present invention should be determined by the technical spirit of the appended claims.

100: body
101: border
102: space
103: flange portion
104: grip holder
110: cell receptor
111: accommodation space
112: first display unit
113: second display unit
200: cover
201: cover border
210: holding part
220: spaced protrusion

Claims (9)

a body having a plurality of cell receiving units accommodating each of the cells and the heating medium solution on the upper surface; and
a cover coupled to the upper side of the body and sealing the plurality of cell receiving units formed on the upper surface of the body;
A plate for freezing and thawing cells in which the receiving space of any one of the plurality of cell receiving units has a larger capacity than the receiving space of the other cell receiving units.
The method according to claim 1,
The cell receptor
A plate for freezing and thawing cells, in which four cells form one column in a line, and two columns are formed on the body.
3. The method according to claim 2,
Among the four cell receptors in a row, the capacity of the cell receptor located on the left is relatively larger than that of the other three cell receptors, and the other three cell receptors have the same capacity. Plates for freezing and thawing of the formed cells.
3. The method according to claim 2,
A plate for freezing and thawing cells in which the four cell receptors forming a line have sequentially smaller storage capacity of the cell receptor from left to right.
5. The method of any one of claims 3 or 4,
Any one of the two rows forms a first display part marked with numbers in order from the cell receiving part with the largest capacity,
The other row is a plate for freezing and thawing cells that forms a second display section with numbers in order from the cell receiving section with the smallest capacity.
The method according to claim 1,
the body is
A plate for freezing and thawing cells that forms an open space at the top by vertically protruding edges along the periphery of the bottom part.
The method according to claim 1,
The cell receptor
A plate for freezing and thawing cells, which is formed in a columnar shape in the space of the body and has a hemispherical concave accommodation space on the upper surface to accommodate the cells and the heating medium solution.
The method according to claim 1,
Forming a flange portion extending outwardly from the outer peripheral surface of the rim of the body,
A plate for freezing and thawing cells having a plurality of grip holders gripped by a user's fingers on the flange portion.
The method according to claim 1,
the cover is
A plurality of cradles are formed on the upper surface of each of the freezing straws,
The plurality of mounting units form a third display unit in which numbers are marked in the order from one side to the other side,
A plate for freezing and thawing cells with a spaced protrusion that separates the body stacked on the upper surface to the corresponding height.

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