CN211505326U

CN211505326U - Electrophoresis sample application appurtenance

Info

Publication number: CN211505326U
Application number: CN202020081161.9U
Authority: CN
Inventors: 王琳; 曾征宇; 郭晓磊
Original assignee: Guangzhou Kingmed Diagnostics Central Co Ltd
Current assignee: Guangzhou Kingmed Diagnostics Central Co Ltd
Priority date: 2020-01-14
Filing date: 2020-01-14
Publication date: 2020-09-15
Anticipated expiration: 2030-01-14

Abstract

The utility model provides an electrophoresis application of sample appurtenance is applied to among molecular biology experiment, genetics experiment, the biochemical experiment, and it is equipped with the sample groove that is used for the holding sample including application of sample dish and base on the application of sample dish, and the base is used for bearing application of sample dish, and application of sample dish can be dismantled in order to change on the base. The sample application disc is supported by the base, so that the sample application disc is easier to transfer, the problem that samples are mixed in a cross mode in the transferring process can be effectively solved, and meanwhile, the disposable sample application disc can be used, and cross contamination among different samples is prevented.

Description

Electrophoresis sample application appurtenance

Technical Field

The utility model relates to an experimental device technical field, concretely relates to electrophoresis sample application appurtenance.

Background

Agarose gel electrophoresis is an electrophoresis method using agarose as a support medium, is used for separating DNA molecules, and is widely applied to molecular biology experiments, genetic experiments or biochemical experiments, such as detection of PCR (polymerase chain reaction) products, nucleotide research and the like.

At present, a sealing film or a thin film glove is adopted as a temporary auxiliary tool before sample application before an electrophoresis experiment. Dropping about 3ul of bromophenol blue on a sealing film or a glove, sequentially and uniformly mixing about 2ul of detection sample with the bromophenol blue to complete preparation of an electrophoresis sample, and washing with clear water for next continuous use after the electrophoresis sample is used up. Because the diaphragm is comparatively frivolous, cause cross mixing to take place between the sample easily in the transfer process, and used repeatedly's diaphragm causes the sample easily and pollutes.

SUMMERY OF THE UTILITY MODEL

The utility model provides an electrophoresis sample application appurtenance aims at solving the easy contaminated problem of electrophoresis sample of preparation among the prior art.

The electrophoresis sample application appurtenance that this embodiment provided includes the sample application dish, be equipped with the sample groove that is used for the holding sample on the sample application dish, electrophoresis sample application appurtenance is still including being used for bearing the base of sample application dish, sample application dish can certainly dismantle in order to change on the base.

In an exemplary embodiment, the electrophoretic deposition aid further comprises a separator provided on the base, a portion of the deposition disc being supportable from the base by prying the separator.

In an exemplary embodiment, the separating member is provided with a positioning portion that allows the spotting plate to be disposed at a predetermined position on the base.

In an exemplary embodiment, a positioning structure is disposed between the base and the spotting plate, and the positioning structure enables the spotting plate to be placed at a predetermined position on the base.

In an exemplary embodiment, the positioning structure includes a protrusion and a groove formed between the spotting disk and the base to be engaged with each other.

In an exemplary embodiment, the number of the sample grooves is a plurality and the sample grooves are distributed in a matrix, a transverse scale and a longitudinal scale are arranged on the base, and an identification lattice formed by the transverse scale and the longitudinal scale corresponds to the distribution of the sample grooves;

when the sample dispensing disc is arranged on the base, each sample slot is marked with different marks by the transverse scale and the longitudinal scale.

In an exemplary embodiment, the spot plate is capable of being disassembled into unit spot plates having at least one of the sample wells.

In an exemplary embodiment, the base is provided with a fixing portion for fixing the reagent vessel.

In an exemplary embodiment, the base includes a table portion and a supporting portion, the supporting portion is configured to support the table portion, the table portion is configured to support the spotting disk, and the fixing portion is a groove body formed on the table portion.

In an exemplary embodiment, the base is provided with a handheld portion for holding.

Implement the embodiment of the utility model provides a, will have following beneficial effect:

this electrophoresis application of sample appurtenance supports the point appearance dish through setting up the base, makes the transfer of point appearance dish easier, can avoid the sample to produce the problem emergence of alternately mixing at the in-process that shifts effectively. Simultaneously set up the sample application dish to install and remove on the base, can use disposable sample application dish simultaneously, prevented the cross contamination between the different samples.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Wherein:

fig. 1 is a schematic top view of an electrophoretic spotting auxiliary tool provided in an embodiment of the present invention;

fig. 2 is a schematic diagram illustrating a top view structure of a sample application disk according to an embodiment of the present invention;

fig. 3 is a schematic top view of a base according to an embodiment of the present invention;

fig. 4 is a schematic top view of another spotting plate provided in the embodiment of the present invention;

fig. 5 is a schematic top view of another sample application disk provided by an embodiment of the present invention;

fig. 6 is a schematic diagram illustrating a top view of a sample application disk on a base according to an embodiment of the present invention;

fig. 7 is a schematic front view illustrating a base according to an embodiment of the present invention;

fig. 8 shows a schematic diagram of a right-view structure of a base provided by an embodiment of the present invention.

In the figure:

100-an auxiliary tool for electrophoresis sample application; 110-spot plate; 111-sample wells; 112-easy-tear line; 120-a base; 120 a-a table portion; 120 b-a support; 121-a catch; 122-lateral scale; 123-longitudinal scale; 124-a fixed part; 125-finger groove; 126-hand-held part; 130-a separator; 131-positioning part.

Detailed Description

In order to facilitate understanding of the present invention, the present invention will be described more fully hereinafter with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

As shown in fig. 1, the present embodiment provides an auxiliary tool 100 for electrophoresis spotting, which can be applied to experiments such as molecular biology experiments, genetics experiments, and biochemical experiments for containing, spotting, and loading samples. The reagents can react on the auxiliary tool 100 for electrophoresis spotting, thereby completing the preparation of the target sample, for example, the sample before the detection of DNA electrophoresis. The electrophoretic spotting assistant tool 100 includes a spotting disk 110 and a base 120.

Referring to fig. 2 and 3, the sample application tray 110 is provided with a sample slot 111 for accommodating a sample, and the base 120 is used for supporting the sample application tray 110 and providing a support for the sample application tray 110. Before preparing a sample, the spotting disk 110 is arranged on the base 120, then the sample preparation is carried out, and after the sample preparation is finished, the spotting disk 110 is carried by the base 120 to be transferred, so that the spotting disk 110 can be prevented from being deformed in the moving process due to the fact that the structure is light and thin, and the samples are prevented from being splashed out and even being mixed.

In addition, the spotting disk 110 can be detached from the base 120, the spotting disk 110 can be used once, and a new spotting disk 110 can be replaced every time spotting, so that cross contamination among samples, especially among different samples, can be effectively prevented.

The number of the sample wells 111 on the spotting plate 110 is plural and distributed in a matrix, and may be the same as the specification of a 96-well plate, having 8 rows and 12 columns, or may be provided as a plate with different rows and columns, and different row and column intervals as required. The sample application tray 110 is in a sheet shape, and the sample slot 111 thereon can be set to 10ul, but can also be set to other capacities, such as 15ul, 20ul, etc., according to the usage requirement. The sample application plate 110 can be made of transparent plastic, the transparent material is convenient to observe, and the plastic material can meet the use requirement of electrophoresis and can also meet the low-cost requirement of disposable use.

In this embodiment, the auxiliary tool for electrophoresis spotting further comprises a separating element 130, the separating element 130 is disposed on the base 120, the base 120 is provided with a clamping portion 121, the separating element 130 is clamped on the base 120 by the clamping portion 121, and the separating element 130 can be levered to a certain degree relative to the base 120. The separating member 130 has a sheet shape, and when the cliche plate 110 is placed on the base 120, the separating member 130 is interposed between the cliche plate 110 and the base 120. The microplate 110 can be partially supported from the base 120 by prying the separator 130, thereby facilitating removal of the microplate 110 from the base 120.

The separating member 130 is in the shape of a handle, and is disposed at a corner of the base 120 to be inclined outward. The clamping parts 121 are clamped at two sides of the separating part 130, the clamping parts 121 are two salient points arranged on the base 120, two notches are arranged at two sides of the separating part 130, and a certain movement allowance exists between the salient points and the notches, so that the separating part can be prized and connected to the base. In another embodiment, the holding portion 121 may also be a connecting lug, and the separating element 130 is provided with two rotating shafts, and the two rotating shafts are rotatably connected to the holding portion 121.

The separating member 130 is provided with a positioning portion 131 that allows the spotting plate to be disposed at a predetermined position on the base. For example, the positioning portion 131 may be an L-shaped boss provided on the separating member, and the positioning of the spotting plate is realized by positioning one corner of the spotting plate 110.

In addition, a positioning structure is further provided between the base 120 and the sample application disk 110, and the positioning structure enables the sample application disk 110 to be placed on a predetermined position of the base 120. Through the setting of location structure, carry out prepositioning in the position of spotting disk 110 on base 120 earlier, then fix handle 112 and accomplish the location on card holder 121, location structure makes the fixed effect of spotting disk 110 on base 120 better.

The positioning structure comprises a protrusion and a groove which are formed between the sample application plate 110 and the base 120 and are mutually embedded, wherein the protrusion is arranged on the sample application plate 110, the groove is arranged on the table top of the base 120, the groove is arranged on the sample application plate 110, and the protrusion is arranged on the table top of the base 120.

Further, in order to make the cliche plate 110 thinner and lighter and to save the material of the cliche plate 110, the thickness of the entire cliche plate 110 is uniform, and the sample wells 111 thereon are formed by the concave-convex structure of the cliche plate 110, thereby forming protrusions on the reverse surfaces of the sample wells 111. Grooves corresponding to the protrusions are formed on the base 120, and the protrusions on the sample application disk 110 and the sample wells 111 are distributed in the same manner, so that the grooves on the base 120 and the sample wells 111 are distributed in the same manner, thereby enabling sufficient positioning between the sample application disk 110 and the base 120.

The base 120 is provided with a horizontal scale 122 and a longitudinal scale 123, and the horizontal scale 122 and the longitudinal scale 123 form an identification lattice corresponding to the distribution of the sample grooves 111. When the spot plate 110 is placed on the base 120, each sample well 111 is marked with a different label by the lateral scale 122 and the longitudinal scale 123. Further, the lateral scales 122 are disposed along the length direction of the matrix of sample wells 111, and the pitch of adjacent marks on the lateral scales 122 is the same as the column pitch of the sample wells 111, thereby serving to mark the columns of the sample wells 111. The longitudinal scale 123 is disposed along the width direction of the matrix of sample grooves 111, and the pitch of adjacent marks on the longitudinal scale 123 is the same as the line pitch of the sample grooves 111, thereby serving to mark the lines of the sample grooves 111. The dot matrix formed by the marks on the lateral scale 122 and the longitudinal scale 123 corresponds one-to-one to the sample grooves 111.

The horizontal scale 122 and the vertical scale 123 may be provided with numbers, letters, numbers, or other symbols and characters. In this embodiment, the horizontal scale 122 is provided with numbers 1-12, and the longitudinal scale 123 is provided with letters A-H.

By arranging the scale, on one hand, each sample groove 111 can be marked, and on the other hand, the number and the positions of the sample grooves 111 can be obtained more intuitively. The lateral scale 122 and the longitudinal scale 123 may be adhered to the base 120 or may be engraved on the base 120.

In this embodiment, the print tray 110 can be disassembled into unit print trays 110 having at least one sample well 111. Therefore, the sample plate 110 can be detached according to the use requirement, and the waste of the sample plate 110 can be effectively reduced when the sample amount is small.

The spotting plate 110 is provided with an easy-tear line 112, and the spotting plate 110 can be detached by tearing the easy-tear line 112. A tear line 112 may be provided between adjacent rows of sample wells 111 to allow the sample trays to be separated between the rows; as shown in fig. 4, a tear line 112 may also be provided between adjacent columns of sample wells 111 to split the sample tray between the columns; as shown in fig. 5, the tear lines 112 may also be disposed between both rows of the sample wells 111 and columns of the sample wells 111 to separate the sample tray into sample tray units having more patterns.

As shown in fig. 6, it should be noted that the separating member 130 is provided at a corner where the lateral scale 122 and the longitudinal scale 123 meet. If the intersection point where the lateral scale 122 and the longitudinal scale 123 extend is at the upper left corner of the base 120, the catch 121 is also disposed at the upper left corner. Thus, when the well plate 110 is divided, particularly when the well plate is divided simultaneously between rows and columns, the marking can be started always in the forward direction of the scale, the number of sample wells 111 can be indicated more intuitively, and the separator 130 can always be caused to function as a stand-off for the well plate 110.

In this embodiment, the base 120 is provided with a fixing portion 124 for placing a reagent container, the reagent container can be a test tube, a measuring cup, a reaction vessel, etc., and the structural specification of the fixing portion 124 can be set according to the type of the experiment and the container required by the experiment. For electrophoresis experiments, a label (Marker) and a Loading buffer solution (Loading buffer) are needed, and the reagent container is a centrifuge tube, also called an EP (eppendorf) tube, and has a capacity of 1.5 ml. The structure of the fixing portion 124 is correspondingly configured according to the centrifuge tube, such as a slot/hole corresponding to the centrifuge tube.

Referring to fig. 7 to 8, the base 120 includes a stage 120a and a supporting portion 120b, the supporting portion 120b is used for supporting the stage 120a, the stage 120a is used for supporting the spotting disk 110, and the fixing portion 124 is a groove formed on the stage 120a for inserting and storing the centrifuge tube. It can be understood that the fixing portion 124 can be a through groove/through hole formed on the table top for inserting a sample tube, or a blind groove formed on the table top, so as to provide better wrapping property for a centrifuge tube inserted therein, so that the centrifuge tube inserted therein has better fixing effect.

The table part 120a has a rectangular plate shape, and a groove corresponding to the protrusion of the sample application plate 110 is formed on the table surface, a longitudinal scale 123 is formed on the left side of the table part 120a, a lateral scale 122 is formed on the top side, a clamping part 121 is formed on the upper left corner, and fixing parts 124 are formed on the upper right corner and the lower left corner. The fixing portion 124 may be a shell-shaped groove recessed from the table portion 120 a. Meanwhile, the lower right corner is provided with a finger groove 125 which has a vortex type convex edge structure and has a certain anti-slip effect. When the stage part 120a is held, the base 120 is pushed and moved by placing the thumb on the finger groove 125.

The supporting portion 120b may be a leg provided under the stage 120a for supporting the stage 120a from the placing surface thereof, so as to move the base 120 by holding the stage 120 a.

The base 120 is provided with a handheld portion 126 for holding, specifically disposed on two sides of the table portion 120a, so as to facilitate carrying the base 120, the handheld portion 126 may be groove-shaped or protrusion-shaped, so as to provide a fastening point for holding, facilitate carrying the base 120, and further improve the stability of movement of the sample tray 110.

The flow of use of the electrophoretic spotting assist tool 100 will now be illustrated:

the first step is as follows: taking out the base 120 and placing the base in front of the electrophoresis apparatus;

the second step is that: selecting the size of the sample application disk 110 according to the amount of the sample, placing the sample application disk 110 on the base 120, and partially clamping the separating member 130 between the sample application disk 110 and the base 120;

the third step: preparation of the electrophoretic sample is started by placing the EP tubes containing the Marker and the Loading buffer at the two fixing portions 124, respectively.

Preparing an electrophoresis sample:

the first step is as follows: sucking quantitative Loading buffer to each sample groove 111;

the second step is that: then taking a quantitative PCR product and mixing with a Loading buffer uniformly;

the third step: the left and right hand-held parts 126 of the hand-held base 120 move smoothly to prevent cross mixing among samples;

the fourth step: sample application;

the fifth step: after the spotting is completed, the separation member 130 is pried to separate the spotting disk 110 from the base 120, and then the spotting disk 110 is removed and thrown into a medical trash can (to prevent cross contamination, the base 120 can be recycled).

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims

1. Electrophoresis application of sample appurtenance, including the application of sample dish, be equipped with the sample groove that is used for the holding sample on the application of sample dish, its characterized in that, electrophoresis application of sample appurtenance still is including being used for bearing the base of application of sample dish, application of sample dish can certainly dismantle in order to change on the base.

2. The electrophoretic deposition aid of claim 1, further comprising a separator disposed on the base, wherein a portion of the deposition disk can be lifted off the base by prying the separator.

3. An auxiliary tool for electrophoresis spotting according to claim 2, wherein a positioning part is provided on the separator, and the positioning part allows the spotting disk to be disposed at a predetermined position on the base.

4. An auxiliary tool for electrophoresis spotting according to claim 1, wherein a positioning structure is provided between the base and the spotting plate, the positioning structure allowing the spotting plate to be placed at a predetermined position on the base.

5. The electrophoretic spotting auxiliary tool of claim 4 wherein the positioning structure comprises interfitting projections and recesses formed between the spotting disk and the base.

6. The auxiliary tool for electrophoresis spotting of claim 1, wherein the number of the sample grooves is plural and distributed in a matrix, the base is provided with a horizontal scale and a vertical scale, and the horizontal scale and the vertical scale form an identification lattice corresponding to the distribution of the sample grooves;

7. The electrophoretic spotting accessory tool of claim 6 wherein the spotting disk is capable of being broken down into unit spotting disks having at least one of the sample wells.

8. An auxiliary tool for electrophoresis spotting according to any of claims 1-7, wherein a fixing portion for fixing a reagent container is provided on the base.

9. The electrophoresis spotting auxiliary tool of claim 8, wherein the base comprises a table portion and a supporting portion, the supporting portion is used for supporting the table portion, the table portion is used for supporting the spotting disk, and the fixing portion is a groove formed on the table portion.

10. An auxiliary tool for electrophoretic deposition according to any of claims 1-7 wherein the base is provided with a hand-held portion for holding.