KR102496752B1 - Fruits of Acanthopanax Sessiliflorus extract including alcoholic liver injury prevention functional ingredients and method for manufacturing it - Google Patents

Abstract

The present invention relates to a composition for inhibiting alcohol-induced liver damage comprising an extract of Acanthopanax chinensis as an active ingredient. The composition of the present invention is effective in reducing blood concentrations of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) and effectively reducing the blood LDH concentration increased by ethanol administration in an animal model that induces alcohol-induced liver damage It can be usefully used as a health functional food for suppression or a treatment for alcoholic liver damage disease.

Description

Fruits of Acanthopanax Sessiliflorus extract including alcoholic liver injury prevention functional ingredients and method for manufacturing it}

The present invention relates to a composition for inhibiting alcoholic liver damage comprising an extract of a fruit extract of quincea cinnamon as an active ingredient.

The liver is the largest organ in the human body, and its main function is to process various substances introduced from outside the body or produced inside the body, and to synthesize and supply important substances. In addition, there are various proteins that play an important role in our body in the blood, and about 90% of them are made in the liver. In addition, the liver plays a role of detoxification and immune system, and various drugs and harmful substances that enter our body are converted into harmless substances in the liver and excreted through urine or bile. In particular, there are immune cells called Kupffer cells in the liver that eat bacteria, toxins, or foreign substances that come from outside the body, decompose them, and discharge them out of the body. As such, the liver performs various and important functions, so when the liver function is severely deteriorated, various problems occur. However, the liver has a vigorous regenerative power, so even if 75% is removed, the size and function are restored after 4 to 6 months.

Depending on the root cause of the disease, liver disease can be classified as liver disease caused by a virus, alcoholic liver disease caused by excessive drinking, toxic liver disease caused by drugs, fatty liver where fat accumulates in the liver, autoimmune liver disease caused by abnormalities in the body's immune system, and toxicity. It is classified as metabolic liver disease caused by excessive accumulation of substances. The main cause of chronic liver disease in Korea is the hepatitis B virus, and type C is on the rise, and is relatively small compared to viral liver diseases. there is. Alcoholic liver disease can be largely divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis according to clinical symptoms, and usually occurs when 60-80g of alcohol is consumed per day for about 10 years. Alcoholic fatty liver is caused by the accumulation of cholesterol and triglycerides in the liver cells due to excessive alcohol intake. It can be recovered soon if you stop drinking, but if you continue to drink, it develops into hepatitis. Alcoholic hepatitis is a state in which liver cell necrosis and inflammation occur, and it shows various symptoms such as fatigue, anorexia, weight loss, jaundice, fever, and right upper quadrant pain. About 40% of patients suffering from this develop alcoholic cirrhosis. Alcoholic cirrhosis is a condition in which recovery to a normal liver is impossible, and it shows various symptoms such as general fatigue, loss of appetite, ascites, esophageal varices, bleeding, hepatic encephalopathy, and coma, and is known to have a poorer prognosis than cirrhosis caused by hepatitis virus.

Alcohol is mainly absorbed in the digestive organs, and about 10% of the alcohol absorbed into the body is excreted through the lungs, urine, and sweat, and 90% is metabolized in the liver. Alcohol moved to the liver through the blood is oxidized to acetaldehyde by several enzymes (Alcohol Dehydrogenase, Microsomal ethanol oxidizing system, Catalase, etc.) produced in the liver, and acetaldehyde is oxidized to acetic acid harmless to the body by enzymes. Acetaldehyde, a metabolite produced by oxidation of alcohol, is a highly reactive toxic substance that inhibits enzyme activity by binding to proteins, damages mitochondria due to an increase in lipid and oxidation, causes glutathione deficiency, and induces pyridoxine, vitamin A, zinc, and selenium. It has been pointed out as a major factor inducing liver damage, such as protein secretion and migration inhibition by inhibition of tubulin polymerization. In addition, since the generation of free radicals by acetaldehyde promotes the synthesis of collagen in the liver, it has been reported to cause liver fibrosis (cirrhosis) in chronic alcohol drinkers. To this end, many products such as alcohol metabolism inhibitors, alcohol metabolism promoters, alcohol degrading enzymes, etc. have been developed or their compositions have been utilized, but most of these products prevent hangovers caused by excessive alcohol consumption rather than preventing and improving liver function. It focuses on resolution.

On the other hand, the oak tree is a short deciduous broad-leaved tree reaching 3 to 4 m in height, and its stem is split close to the ground and grows widely, and its twigs are grayish brown and have few thorns. The leaves are placed opposite each other and form a palm shape with 3 to 5 leaf pieces, and the leaf pieces are egg-shaped or oval-shaped, 6-15 cm long. There are no hairs on the surface of the leaves, and there are fine hairs only on the veins on the back side. At the edge, large teeth and small teeth are arranged overlapping each other. The flowers bloom in umbrella-like clusters at the ends of newly grown branches. Flowers are light purple, have 5 petals, and are about 3mm in diameter. After the flowers fade, the elongated, oval-shaped, watery fruits run together and turn black in autumn. The root or bark is mainly used as a medicine, and has effects such as tonic, pain relief, and wind. There is no report on the effects of preventing or treating alcoholic liver disease, such as paralytic pain caused by wind and dampness, rheumatism, back pain, infidelity, and beriberi.

Accordingly, the inventors of the present invention have made efforts to develop natural materials effective for the treatment of alcoholic liver damage without side effects, and have found that the extract of the fruit extract of the present invention is effective in preventing or treating alcoholic liver damage, and preventing or treating alcoholic liver damage using the same. The therapeutic composition was completed.

An object of the present invention is to provide a composition for inhibiting alcoholic liver damage comprising an extract of quinoa fruit as an active ingredient.

In addition, another object of the present invention is to provide a health functional food composition for inhibiting alcoholic liver damage containing a fruit extract of quinoa fruit.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating alcoholic liver damage containing an extract of a fruit extract of quinoa.

In order to achieve the above purpose,

The present invention provides a composition for inhibiting alcoholic liver damage comprising an extract of a fruit extract of Chinese apricot cinnamon as an active ingredient.

In addition, the present invention provides a health functional food composition for inhibiting alcoholic liver damage containing an extract of a fruit of Ogapi.

In addition, the present invention provides a pharmaceutical composition for preventing or treating alcoholic liver damage comprising an extract of a fruit extract of quinoa.

The present invention relates to a composition for inhibiting alcohol-induced liver damage, comprising an extract of the fruit extract of cucumber fruit as an active ingredient. aminotransferase) and AST (aspartate aminotransferase), and effectively reduces the blood LDH concentration increased by ethanol administration, so that the liver damage inhibitory effect is excellent, so the composition of the present invention is a health functional food composition for preventing alcoholic liver damage or It can be used as a material for a pharmaceutical composition for preventing or treating alcoholic liver disease.

Figure 1 is a diagram showing the cell damage protective effect of the extract of the fruit extract in liver cancer (HepG2) cells.
Figure 2 is a view showing the change in blood concentration of GOT / AST of the extract of the fruit extract of the present invention in an animal model.
Figure 3 is a diagram showing the change in blood concentration of GPT / ALT of the extract of the fruit extract of the present invention in an animal model.
Figure 4 is a diagram showing the change in blood LDH concentration of the extract of cucumber fruit extract.
Figure 5 is a diagram showing the weight of the liver (liver) measured in order to confirm the long-term weight loss change by administration of the extract of the fruit extract.
Figure 6 is a diagram showing the measurement of the weight of the spleen (spleen) in order to confirm the long-term weight loss change by administration of the extract of the fruit extract.

Hereinafter, preferred implementations of the present invention will be described in detail. However, it should be understood that this invention may be embodied in many different forms and is not limited to the described embodiments. It should be noted that the embodiments of the present invention described below are intended to sufficiently convey the spirit of the present invention to those skilled in the art.

In addition, the terms (terminology) used in this specification are terms used to appropriately express preferred embodiments of the present invention, which may vary according to the intention of a user or operator or customs in the field to which the present invention belongs. Therefore, definitions of these terms should be made based on the contents throughout this specification. Throughout the specification, when a certain component is said to "include", it means that it may further include other components without excluding other components unless otherwise stated.

Throughout this specification, '%' used to indicate the concentration of a particular substance is solid/solid (w/w) %, solid/liquid (w/v) %, and Liquid/liquid is (v/v) %.

The present invention provides a health functional food composition for inhibiting alcohol-induced liver damage, comprising an extract of quinoa fruit as an active ingredient.

In the present invention, the extract can be extracted using any one part selected from the stem, fruit or root of the cucumber, but among the above-mentioned parts, the extract extracted from the fruit of the cucumber has a hepatocellular protective effect, GOT/AST And GPT / ALT level reduction, LDH concentration reduction effect may be more excellent.

The term "extract" used in the present invention refers to a preparation obtained by extracting cucumbers with an appropriate extraction solvent and evaporating the solvent to concentrate, but is not limited thereto, an extract obtained by extraction treatment, a dilution of the extract, or It may be a dried product obtained by drying a concentrate or an extract, or a crude or purified product thereof. The Ogapi extract can be prepared using a general extraction method, separation and purification method known in the art. The extraction method is not limited thereto, but preferably, methods such as hot water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, or ultrasonic extraction may be used.

The extract according to the present invention may be obtained by extraction and separation from nature using an extraction and separation method known in the art, and the "extract" defined in the present invention is extracted from cucumber skin using an appropriate solvent, For example, a crude extract, a polar solvent-soluble extract, or a non-polar solvent-soluble extract are all included. Any suitable solvent for extracting the extract from the cucumber may be used as long as it is a pharmaceutically acceptable organic solvent, and water or an organic solvent may be used, but is not limited thereto. For example, purified water, methanol ( Alcohols having 1 to 4 carbon atoms including methanol, ethanol, propanol, isopropanol, butanol, etc., acetone, ether, benzene, chloroform ( chloroform), ethyl acetate, methylene chloride, hexane and cyclohexane, etc. may be used alone or in combination.

As an extraction method, any one of methods such as hot water extraction, cold brew extraction, reflux cooling extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, and compression may be selected and used. In addition, the desired extract may be additionally subjected to a conventional fractionation process or may be purified using a conventional purification method.

There is no limitation on the preparation method of the extract of the present invention, and any known method may be used. For example, the extract included in the composition of the present invention can be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying of the primary extract extracted by the above-described hot water extraction or solvent extraction method.

The term "composition" used in the present invention means a mixture in which other chemical components such as diluents or carriers are mixed with the cucumber extract of the present invention.

The health functional food composition of the present invention can be prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup effervescent tablet, and beverage, or can be ingested to prevent or improve liver damage. If it is, it is not particularly limited.

When using the health functional food composition of the present invention as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to conventional methods.

Active ingredients can be appropriately used depending on their purpose of use (prevention or improvement). In general, it is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the health functional food composition of the present invention when preparing food or beverage. However, in the case of long-term intake for the purpose of health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of food. Examples of foods to which the functional food composition may be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea There are drinks, vitamin complexes, and the like, and includes all health foods in a conventional sense.

In addition, the health functional food composition of the present invention can be made into food, particularly functional food. The functional food of the present invention includes components commonly added during food preparation, and includes, for example, proteins, carbohydrates, fats, nutrients, and seasonings. For example, when prepared as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to active ingredients. The natural carbohydrates are monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrins, cyclodextrins, etc.) or sugar alcohols (eg, maltose, sucrose, etc.) , xylitol, sorbitol, erythritol, etc.) are preferred. As the flavoring agent, natural flavoring agents (eg, thaumatin, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent used in beverages and the like.

In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of alcoholic liver damage disease containing the extract of the fruit extract of quincea cinnamon as an active ingredient.

In the present invention, the alcoholic liver damage disease is alcoholic fatty liver, alcoholic hepatitis or alcoholic cirrhosis, but is not limited thereto.

The pharmaceutical composition of the present invention may further include suitable carriers, excipients or diluents commonly used in the preparation of pharmaceutical compositions. The pharmaceutical dosage form of the composition according to the present invention may be used alone or in combination with other pharmacologically active compounds as well as in suitable combinations.

The pharmaceutical composition according to the present invention may be formulated and used in the form of oral preparations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, aerosols, external preparations, suppositories and injections according to conventional methods, respectively. . Carriers, excipients and diluents that may be included in the pharmaceutical composition include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and various compounds or mixtures including mineral oil and the like.

When formulated, it is prepared using diluents or excipients such as commonly used fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc. These solid preparations include at least one excipient, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. is prepared by mixing In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid preparations for oral use include suspensions, solutions for oral use, emulsions, syrups, etc. In addition to water and liquid paraffin, which are commonly used simple diluents, various excipients such as wetting agents, sweeteners, aromatics, and preservatives may be included. .

Formulations for parenteral administration include sterilized aqueous solutions, non-aqueous solvents, suspensions, emulsions, freeze-dried formulations, and suppositories. Propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used as non-aqueous solvents and suspending agents. As a base for the suppository, Witepsol, Macrogol, Tween 61, cacao butter, laurin paper, glycerogelatin, and the like may be used.

The preferred dosage of the pharmaceutical composition of the present invention varies depending on the condition and body weight of the patient, the severity of the disease, the drug type, the route and duration of administration, but can be appropriately selected by those skilled in the art. The pharmaceutical composition of the present invention can be administered to mammals such as rats, mice, livestock, and humans through various routes. All modes of administration can be envisaged, for example by oral, rectal or intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebroventricular injection.

Hereinafter, the present invention will be described in more detail using preparation examples and examples. These Preparation Examples and Examples are only for explaining the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited thereto.

Example 1. Preparation of Ogapi Fruit Extract

1 kg of hot air-dried cucumber fruit was powdered, mixed with 3 L of 50% fermented alcohol, and stirred at room temperature for 24 hours. The above process was repeated twice with 3L of 50% fermented alcohol. After filtering on a filter paper and concentrating in a vacuum condition, an ethanol extract of the fruit of a cucumber plant was obtained.

Experimental Example 1. Liver cancer cell toxicity test

The extract of the fruit extract prepared in Example 1 was administered to liver cancer (HepG2) cells at concentrations of 50 μg/ml, 100 μg/ml, 200 μg/ml, and 300 μg/ml, and after culturing for 48 hours, MTT assay was performed. Cytotoxicity was measured.

As a result, as shown in FIG. 1, it was confirmed that the extract of the present invention had no significant cytotoxicity up to a concentration of 200 μg/ml.

Experimental Example 2. Confirmation of Alcoholic Liver Damage Inhibiting Activity of Acanthopanax Fruit Extract

In order to confirm the alcohol-induced liver damage inhibitory activity of the extract of the fruit of Ogapi, the experiment was conducted as follows.

GOT/AST and GPT/ALT were quantified in the serum of mice orally administered 1 mg/mouse and 3 mg/mouse once a day for a total of 10 times from 3 days before ethanol administration to confirm the liver disease inhibitory activity of the samples. At this time, blood GPT/ALT, GOT/AST, and LDH were quantified using a serum sample on day 1 after ethanol administration was terminated.

As a result, as shown in FIGS. 2 and 3, the levels of GOT/AST and GPT/ALT in the blood, which were elevated by ethanol administration, were all in the experimental group (1 mg/mouse and 3 mg/mouse dose groups) administered with the cucumber fruit extract. It was confirmed that it was effectively reduced.

In addition, when hepatocytes are damaged, LDH (Lactate dehydrogenase) enzyme present in hepatocytes is released into the blood and its concentration increases.

As a result, as shown in FIG. 4 , it was confirmed that the group administered with the extract of the cucumber fruit extract effectively reduced the blood LDH concentration increased by ethanol administration, thereby inhibiting liver damage.

Experimental Example 3. Measurement of Toxic Expression Level of Organs of Acanthopanax Fruit Extract

In order to measure the toxicity expression level of the long-term organ extract, the experiment was conducted as follows.

Experimental groups were divided into normal, ethanol (EtOH)-treated, and ethanol-treated groups. In order to observe the effect of preventing liver damage according to the concentration of the extract of the cucumber fruit extract, the extract of the cucumber fruit extract was orally administered to the ethanol-treated group at concentrations of 1 mg/mouse and 3 mg/mouse, respectively, once a day for a total of 10 times. Based on the initial weight of the mouse, the weight after 10 days of ethanol administration was measured to measure the weight gain rate.

As a result, as shown in FIGS. 5 and 6, it was confirmed that there was no significant change in the weight of the liver and spleen before and after administration of the extract of the extract of the fruit extract, and it was confirmed that the weight reduction of the organ by the administration of the extract of the extract of the fruit extract was not significantly affected. did

Although the preferred embodiments of the present invention have been described in detail above, the present invention is not limited to the specific embodiments described above, and is common in the art to which the present invention pertains without departing from the gist of the present invention claimed in the claims. Of course, various modifications and improvements are possible by those with knowledge of, and these modifications and improvements should not be individually understood from the technical spirit or prospect of the present invention.

Claims (6)

A health functional food composition for inhibiting alcoholic liver damage containing 50% ethanol extract of Acanthopanax Sessiliflorus fruit,
Wherein the alcoholic liver damage disease is alcoholic fatty liver, alcoholic hepatitis or alcoholic cirrhosis. delete delete According to claim 1,
The 50% ethanol extract of the cucumber fruit is a health functional food composition for inhibiting alcoholic liver damage comprising an extract of cucumber extract showing a reducing effect on at least one selected from blood GOT / AST, blood GPT / ALT, and blood LDH concentration elevated by ethanol administration . Acanthopanax Sessiliflorus A pharmaceutical composition for the prevention or treatment of alcoholic liver damage disease comprising 50% ethanol extract of fruit as an active ingredient,
Wherein the alcoholic liver damage disease is alcoholic fatty liver, alcoholic hepatitis or alcoholic cirrhosis. delete

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