KR102480651B1 - Process for breeding animal with organic germanium-ion water - Google Patents

Abstract

The present invention relates to a breeding method for animals containing germanium components using organic germanium ionized water, and more particularly, to synthesize insoluble metal germanium into organic germanium in an ionic state to provide a state of good absorption for animals. , The oxygen group of germanium oxide, which is easily accessible, is combined with a chlorine group to synthesize germanium tetrachloride, and the acid (RCOOH) group (or hydroxyl group (ROH)) of an amino acid is used as an anion to synthesize germanium ions in a state that can be dissolved in water. By making it a counterpart (RCOO ^- , or RO ^- ) Cationic germanium (Ge ⁴⁺ ) and a method for breeding animals containing germanium components using organic germanium ionized water, which is synthesized in an ionic state that is well soluble in water and applied to animals to reduce harmfulness. It is about. According to the present invention, a functional product having safety and usefulness can be provided by converting insoluble metal germanium into organic germanium in an ionic state and providing it in a state having good absorbability for animals.

Description

Method for breeding animals containing germanium using organic germanium ion water {Process for breeding animal with organic germanium-ion water}

The present invention relates to a breeding method for animals containing germanium components using organic germanium ionized water, and more particularly, to synthesize insoluble metal germanium into organic germanium in an ionic state to provide a state of good absorption for animals. , The oxygen group of germanium oxide, which is easily accessible, is combined with a chlorine group to synthesize germanium tetrachloride, and the acid (RCOOH) group (or hydroxyl group (ROH)) of amino acids is used as an anion to synthesize germanium ions in a state that can be dissolved in water. By making it a counterpart (RCOO ^- , Or RO ^- ) Cationic germanium (Ge 4+ ) and a method for breeding animals containing germanium components using organic germanium ionized water that reduces harmfulness by synthesizing ionic germanium (Ge ⁴⁺ ) and water-soluble ions and applying the synthesized organic germanium ions to animals It is about.

Germanium is a carbon group element belonging to group 4B of the periodic table. Organic germanium with high purity holds and accumulates cancer cells, toxins, pollutants, heavy metals, etc. It is known to be very safe because it is completely excreted from the human body through urine without any toxicity [Asai Germanium Research Institute, Tokyo, Japan].

In addition, it has been reported that organic germanium acts as an interferon derivative in the human body, suppresses tumors and malignant tumors, lowers blood pressure in animals, or has analgesic effects.

On the other hand, Korean Patent Registration No. 10-400139 discloses that a composition for livestock supplementary feed containing germanium ore powder was mixed with general chicken feed at a ratio of 1:3 and fed to chickens for 25 days. It is confirmed that the production rate, egg weight, and eggshell strength of the eggs have improved after feeding. However, in the above registered patent, as in the present invention, when a small amount of germanium-containing supplementary feed composition is mixed and raised for a predetermined period of time, germanium is concentrated in the meat quality of chicken meat, mentioning how it affects humans who eat the chicken is not at all

In addition, Korean Patent No. 10-417241 discloses a method for producing germanium activated eggs by micropowdering mineral form of germanium and mixing it with a formulated feed and feeding it to chickens. However, when the fine powder germanium in the form of inorganic matter is mixed and used in the formulated feed, the absorption rate in the chicken's body is reduced, and the human body's absorption rate is similarly lowered compared to the expensive production cost when eggs produced by this are consumed by humans. There is a problem of low effectiveness, and there is also a risk problem for chicken liver following the intake of a large amount of germanium in a short period of time.

Therefore, various studies for simple and efficient use of organic germanium are still required.

Korean Publication No. 10-2006-0008191 (January 26, 2006) Korea Registration No. 10-0791015 (December 26, 2007) Korea Registration No. 10-0883012 (February 03, 2009)

As a result of intensive research in order to solve the problems of the prior art and provide a product with secured functionality, the present inventors synthesized insoluble metal germanium into organic germanium in an ionic state and provided it as a water-soluble product, as will be described later, so that animals The present invention was completed by discovering that a useful and safe product can be provided with low harmfulness and improved absorbency to animals when applied to.

Therefore, in one aspect, an object of the present invention is to provide a breeding method for animals containing germanium components using organic germanium ionized water having good absorbency and reduced harmfulness to animals by synthesizing insoluble metal germanium into organic germanium in an ionic state. is in doing

The purpose of the present invention as above is

a) heating and reacting germanium oxide with hydrochloric acid to obtain germanium tetrachloride;

b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;

c) diluting the organic germanium ions with purified water to obtain an organic germanium aqueous solution; and

d) injecting the organic germanium aqueous solution into an animal to obtain an animal or a by-product containing organic germanium;

According to the present invention, functional animals or by-products having safety and usefulness can be provided by converting insoluble metal germanium into organic germanium in an ionic state and providing it in a state with good absorbability for animals.

1 is a process chart for a breeding method for animals containing organic germanium of the present invention.
2 is a diagram explaining a synthesis process of organic germanium ions according to the present invention.
3 is a test result of measuring the content of germanium synthesized using ascorbic acid.
4 is a test result of measuring the content of germanium synthesized using low molecular weight collagen.
5 is a test result of measuring the germanium content of eggs obtained from laying chickens.

The present invention, in one aspect,

a) heating and reacting germanium oxide with hydrochloric acid to obtain germanium tetrachloride;

b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;

c) diluting the organic germanium ions with purified water to obtain an organic germanium aqueous solution; and

d) obtaining an organic germanium-containing animal or a by-product thereof by injecting the organic germanium aqueous solution into the animal;

The present invention, in a further aspect,

The organic compound provides an animal breeding method using organic germanium, characterized in that ascorbic acid, cysteine, salicylic acid, sialyllactose or amino acids.

The present invention, in another further aspect,

Step a) is carried out by heating and reacting with hydrochloric acid,

Step b) reacts germanium tetrachloride with an organic compound having a hydroxy group or carboxy group, acetone, and baking soda under reflux, and then firstly removes acetone using a rotary condenser, and then pumps with a vacuum pump to secondarily remove acetone. It provides a method for breeding animals using organic germanium, characterized in that.

The present invention, in another further aspect,

The animal is poultry including chickens or pigs, livestock livestock including cattle, insects including bees or brown mealworms, and fish including eels, and provides a method for breeding animals using organic germanium.

Hereinafter, a method for breeding animals containing organic germanium according to the present invention will be described in more detail with reference to the accompanying drawings.

The terms or words used in this specification and claims should not be construed as being limited to ordinary or dictionary meanings, and the inventors may appropriately define the concept of terms in order to explain their invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that there is. Therefore, the embodiments described in this specification and the configurations described in the drawings are only the most preferred embodiments, and do not represent all of the technical spirit of the present invention, so various equivalents and equivalents that can replace them at the time of this application It should be understood that variations may exist.

The present invention synthesizes insoluble metal germanium into organic germanium in an ionic state to provide a state with good absorption for animals. In order to synthesize germanium ions in a soluble state, the acid (RCOOH) group (or hydroxyl group (ROH)) of an amino acid is made into an anion counterpart (RCOO ^- , Or RO ^- ) It has a technical feature in that it is synthesized in an ionic state that is well soluble in cation germanium (Ge ⁴⁺ ) and water, and the synthesized organic germanium ion is applied to animals to reduce harmfulness.

As shown in the figure, the breeding method for animals containing organic germanium of the present invention includes a step of preparing germanium tetrachloride, a step of preparing organic germanium ions, a step of preparing an aqueous solution, and a step of applying to animals. Hereinafter, each individual step will be described in detail.

a) Germanium tetrachloride manufacturing step

In order to make metal germanium easily soluble in water, a process of synthesizing germanium oxide into chlorine germanium is required first. Germanium tetrachloride is obtained by heating and reacting germanium oxide with hydrochloric acid as shown in Chemical Formula 1 below. For this purpose, it may be desirable to synthesize germanium tetrachloride by combining a chlorine group with germanium oxide, which is easy to purchase, instead of oxygen. The heating time is suitable for 20 to 40 minutes, and the temperature suitable for the reaction is 90 to 120 ° C. Subsequently, the reactant is distilled at 80 to 90 ° C using a distillation apparatus to obtain germanium tetrachloride.

b) Preparation of germanium ion using germanium tetrachloride

b1) Preparation of germanium ions bonded to hydroxy groups

In order to synthesize organic germanium ions that are easily soluble in water, it is necessary to convert germanium chloride into an ionic state using an amino acid or the like.

In the present embodiment, the anion of the hydroxyl group (-OH) of ascorbic acid combines with the germanium cation to form a germanium ion.

As shown in Formula 2 below, acetone, baking soda, and an organic compound having a hydroxyl group, such as ascorbic acid, are stirred at room temperature for 5 to 20 minutes. Subsequently, germanium tetrachloride is added to the reaction container and reacted under reflux for 6 to 10 hours to obtain organic germanium in ionic form. In this reaction, hydrochloric acid is produced as a by-product, and neutralized with baking soda (NaHCO ₃ ) to remove the hydrochloric acid.

After the reaction is complete, the acetone is carefully removed using a rotary evaporator, since solids may be duffed. It is then pumped in a vacuum pump for 4 hours to remove trace amounts of acetone.

b2) Production of germanium ions bonded to carboxy groups

In order to synthesize organic germanium ions that are easily soluble in water, it is necessary to convert germanium chloride into an ionic state using an amino acid or the like.

In this embodiment, the anion of the carboxy group of the low-molecular-weight collagen combines with the germanium cation to form a germanium ion.

As shown in Formula 3 below, acetone, baking soda and low molecular weight collagen are added and stirred at room temperature for 5 to 20 minutes. Subsequently, germanium tetrachloride is added to the reaction vessel and reacted under reflux for 6 to 10 hours to obtain organic germanium in ionic form. In this reaction, hydrochloric acid is produced as a by-product, and neutralized with baking soda (NaHCO ₃ ) to remove the hydrochloric acid.

After the reaction is complete, the acetone is carefully removed using a rotary evaporator, since solids may be duffed. It is then pumped in a vacuum pump for 4 hours to remove trace amounts of acetone.

The organic compound is not limited thereto, and may include ascorbic acid, cysteine, salicylic acid, and sialyllactose.

c) Preparation of an organic germanium aqueous solution

Organic germanium ions are obtained in the form of an aqueous solution using distilled water or the like. A suitable concentration of organic germanium ions in the aqueous solution may be preferably 10 to 10,000 ppm.

d) injection into animals

Organic germanium as above can be injected in various ways to various animals.

For example, organic germanium can be supplied by being included in the composition of drinking water, feed, or feed.

In addition, it can be applied to poultry including chickens or livestock animals such as pigs and cows after first ingestion using vectors such as earthworms, snakes, and larvae.

In addition, it can be used for breeding insects, including honey and brown gourds, and can also be used for growing fish, including eels.

As an example of egg production, in order to produce eggs containing organic germanium components, germanium ionized water that is well soluble in water is made, and germanium ionized water is supplied to laying hens as drinking water for 1 to 3 weeks so that organic germanium ionized water can be applied to animals. there is.

It may be desirable to set the germanium ionized water to a concentration of 200ppm and supply the feeding amount at 1 to 10,000 mL per animal.

<Example>

Hereinafter, the present invention will be described in more detail through examples. These examples are only for explaining the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Example 1: Preparation of germanium tetrachloride

After installing a condenser in a 500mL 3-neck flask, the condenser's cooling water was maintained at -40°C. 2 g of germanium oxide (GeO2) was added to 300 mL of 6N hydrochloric acid and heated for 30 minutes. After removing the condenser and installing a distillation device, the mixture was distilled at 86.5° C. to obtain 3 g of germanium tetrachloride (GeCl ₄ ).

Example 2: Preparation of germanium ion using germanium tetrachloride

500mL of dried acetone, 4.1g of ascorbic acid, and 2g of finely powdered baking soda (NaHCO ₃ ) were added to a 1000mL dry round bottom flask and stirred at room temperature for 10 minutes. Germanium tetrachloride (GeCl ₄ ) 1g (0.53mL) was added to the reaction vessel and refluxed for 8 hours. After the reaction was completed, acetone was carefully removed using a rotary evaporator, and then acetone was removed using a vacuum pump for 4 hours to obtain 2.5 g of organic germanium ions. 500 mL of distilled water was added to the flask from which the acetone was removed to obtain organic germanium ionized water.

Example 3: Preparation of germanium ion bound to carboxylic acid

500mL of dried acetone, 6.6g of low molecular weight collagen (Gly-Pro-Hyp), and 2g of finely powdered baking soda (NaHCO ₃ ) were added to a 1000mL round flask and stirred at room temperature for 10 minutes. Germanium tetrachloride (GeCl ₄ ) 1g (0.53mL) was added to the reaction vessel and refluxed for 8 hours.

After the reaction was completed, acetone was carefully removed using a rotary evaporator, and acetone was removed using a vacuum pump for 4 hours to obtain 2.5 g of organic germanium ions. 500 mL of distilled water was added to the flask from which the acetone was removed to obtain organic germanium ionized water.

Example 4: Production of eggs

In order to produce eggs containing germanium, a laying hen cage (4m x 4m x 2m) is built, and germanium ionized water is put in a barrel with a nipple waterer. The organic germanium ion water of Example 2 was supplied to the laying hens for 1 to 3 weeks of drinking water. Germanium ionized water is set to a concentration of 200ppm, and the feeding amount is set to 120mL per animal. Five healthy laying hens were selected and the laying eggs were collected on the 1st week and submitted to ICP emission spectroscopic analysis.

Test Example 1: Germanium component analysis

Components obtained in Example 2 were analyzed by requesting ICP (Inductively Coupled Plasma) emission spectroscopy. 3 is a test result of measuring the content of germanium synthesized using ascorbic acid, and it was found that the content of ionic germanium was 1042.8 mg/kg.

Test Example 2: Germanium component analysis

The components (germanium ions prepared using carboxylic acid) obtained in Example 3 were subjected to ICP (Inductively Coupled Plasma) emission spectroscopy analysis to analyze the germanium components. 4 is a test result of measuring the content of germanium synthesized using low molecular weight collagen. As a result of the test, the content of ionic germanium was found to be 1440.6 mg/kg.

Test Example 3: Germanium component analysis

Eggs obtained in Example 4 were analyzed for their components by requesting emission spectroscopy. 5 is a test result of measuring the germanium content of eggs produced from chickens supplied with germanium ionized water. The germanium content of eggs was found to be 4.6 mg/kg.

Test Example 4: Acute toxicity test

A toxicity test was performed on the organic germanium ionized water according to Example 2, and the presence or absence of toxicity was observed. Experimental animals were 60 male and female SD rats weighing 105 ± 4 g and 95 ± 3 g, aged 4 and 5 weeks, and tested using the extract of the present invention and distilled water as a negative control. First, the rats were placed in a laboratory breeding box with a temperature of 22 ± 2 ° C, a relative humidity of 53 ± 2%, and a light-dark cycle of fluorescent lighting (09:00 on-18:00 off), illumination conditions of 150-300 Lux for about 1 After acclimatization over a period of about a week, only healthy animals were selected, divided into groups to match the average weight, and forced orally administered once a day in an amount of 20 ml/kg for 14 days, and then changes in general condition and symptoms of poisoning , motility, appearance, autonomic nerves, weight change, and the presence or absence of dead animals were checked.

According to the experimental results, there was no significant change in body weight during the experimental period, but there was no significance, and no dead animals were observed even though the highest concentration of 20ml per kg was selected for the amount of sample, which is the maximum amount that can be administered to test animals. It was not possible to calculate the lethal dose, so the LD ₅₀ was found to be 20ml/kg BW or more, and no specific general symptoms or specific lesions were observed at autopsy.

As described above, the present invention suggests the direction of using organic germanium, and provides an opportunity for additional interest and roles of those involved in the manufacturing industry to create jobs and to lead the well-being food culture. judged In addition, since social and cultural health-related issues continue to emerge in the present and future in the mass media, many palatable and functional foods such as the present invention have been developed, suggesting a direction to solve various environmental and social problems.

As described above, the present invention has been described by the limited embodiments and drawings, but the present invention is not limited to the above embodiments, and those skilled in the art in the field to which the present invention belongs can make various modifications and transformation is possible Therefore, the spirit of the present invention should be grasped only by the scope of the claims described below, and all equivalent or equivalent modifications thereof will be said to belong to the scope of the spirit of the present invention.

Claims (5)

a) heating and reacting germanium oxide with hydrochloric acid to obtain germanium tetrachloride;
b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;
c) obtaining an aqueous solution by diluting the organic germanium ion with purified water and dissolving the organic germanium to a final concentration of 10 to 10000 ppm; and
d) supplying the organic germanium aqueous solution to an animal to obtain an animal containing organic germanium or a by-product thereof;
The organic compound is an animal breeding method using organic germanium, characterized in that ascorbic acid or low molecular weight collagen. delete The method of claim 1,
Step a) is carried out by heating and reacting with hydrochloric acid,
Step b) reacts germanium tetrachloride with an organic compound having a hydroxy group or carboxy group, acetone, and baking soda under reflux, and then firstly removes acetone using a rotary condenser, and then pumps with a vacuum pump to secondarily remove acetone. Animal breeding method using organic germanium, characterized in that. The method of claim 1,
In step d), the organic germanium solution is supplied to the animal at a concentration of 200 ppm and 1 to 10,000 mL per animal to obtain an animal containing organic germanium or a by-product thereof. animal breeding methods. The method of claim 1,
The animal breeding method using organic germanium, characterized in that the animal is poultry including chickens or pigs, cattle livestock animals, insects including bees or brown geese, or fish including eels.

Images