KR101566588B1 - Feed Composition for domestic animal made of phosphorus from animal manure - Google Patents

Abstract

The present invention relates to a livestock feed composition containing phosphorus extracted from livestock manure as an active ingredient and a method for enhancing the recycling efficiency of livestock feed, and provides a method for efficiently extracting and recovering phosphorus in livestock feed , The present invention provides a functional livestock feed containing EPT liquefied EP 1 and heat-treated livestock manure (EP 2) for the purpose of removing ammonia toxic to animals from a calcium phosphate replacement material recovered from livestock manure In case that the livestock feed provided by the present invention is fed to the actual livestock, commercial livestock feed can be substituted for the commercial livestock feed.

Description

[0001] The present invention relates to a feed composition for livestock manure,

The present invention relates to a feed composition for livestock containing phosphorus extracted from livestock manure as an active ingredient.

For the normal growth of animals, the addition of phosphoric acid feedstuffs in feed is essential and phosphorous is required in various industries. However, currently, the reserves of minerals containing phosphates in the world are about 9 billion tons, of which about 50% are concentrated in Africa, and the amount of phosphorus reserves is rapidly decreasing as people procure from all over the world. In Korea, In fact.

In addition, production after 2030 is projected to decrease sharply and the whole world is expected to suffer shortage of the world, and it is expected to be completely exhausted by 2090.

Therefore, it is urgent to prepare alternative sources of phosphoric acid, but the research is still small.

Korean Patent Publication No. 2004-0069395

Accordingly, an object of the present invention is to provide a feed composition for livestock containing phosphorus extracted from livestock manure as an active ingredient.

Another object of the present invention is to remove ammonia toxic to animals from a calcium phosphate replacement material recovered from livestock manure by (a) microwave treatment for 1 to 10 minutes at 60 to 100 Hz / g, Preparing an ultrasonic treated phosphate substitute (EP 1); And (b) adding to the phosphate substitute (EP 1) of step (a) the corn, wheat, gluten, corn steep liquor (DDGS), whole soybean, soybean meal, vegetable oil, limestone, salt, Which comprises the step of adding DL-methionine, L-lysine-sulphate, threonine, a vitamin mix, a mineral mix, Clinacox, Lactovita and / or Maduramycin And to provide a feed manufacturing method.

It is another object of the present invention to provide a method for removing ammonia that may cause toxicity to an animal from calcium phosphate replacement material extracted and recovered from animal manure by (a) drying or painting at 100 to 600 ° C for 10 minutes to 10 hours To produce a powdered phosphate substitute (EP 2); And (b) adding to the powdered phosphate substitute (EP 2) of step (a) the corn, wheat, gluten, DDGS, whole soybean, soybean meal, L-lysine-sulphate, threonine, a vitamin mixture, a mineral mixture, Clinacox, Lactovita and / or Maduramycin, And a method for producing a feed for livestock.

In order to achieve the above object, the present invention provides a feed composition for livestock containing phosphorus extracted from livestock manure as an active ingredient.

In one embodiment of the present invention, the phosphorus may be a microwave treated at 60 to 100 Hz / g for 1 to 10 minutes.

In one embodiment of the present invention, the phosphorus may be dried or crystallized at 100 to 600 ° C for 10 minutes to 10 hours.

Also, the present invention provides a method for producing a phosphate-containing material (EP 1) comprising: (a) preparing an ultrasonic treated phosphate substitute (EP 1) by microwave treatment at 60 to 100 Hz / g for 1 to 10 minutes; And (b) adding to the livestock manure (EP 1) of the step (a) the corn, wheat, gluten, DDGS, whole soybean, soybean meal, Which comprises the step of adding DL-methionine, L-lysine-sulphate, threonine, a vitamin mix, a mineral mix, Clinacox, Lactovita and / or Maduramycin Thereby providing a feed production method.

In one embodiment of the present invention, the extreme ultrasound treated phosphate substitute (EP 1) may contain 5 to 15 wt% of calcium, 10 to 25 wt% of phosphorus, and 0.5 to 2 wt% of magnesium.

Further, the present invention provides a method for producing a phosphoric acid substitute (EP 2) comprising: (a) drying at 100 to 600 ° C for 10 minutes to 10 hours to prepare a powdered phosphate substitute (EP 2); And (b) adding to the powdered phosphate substitute (EP 2) of step (a) the corn, wheat, gluten, DDGS, whole soybean, soybean meal, L-lysine-sulphate, threonine, a vitamin mixture, a mineral mixture, Clinacox, Lactovita and / or Maduramycin, The present invention provides a method for producing a feed for livestock.

In one embodiment of the present invention, the phosphoric acid replacement material (EP 2) powdered by drying or painting may contain 5 to 15 wt% of calcium, 10 to 30 wt% of phosphorus, and 5 to 15 wt% of magnesium.

In one embodiment of the present invention, the composition comprises 35 to 40 wt% of corn, 10 to 20 wt% of wheat, 1 to 3 wt% of gluten, 2 to 6 wt% of corn main anchoring (DDGS) 1 to 3 wt% of limestone, 1 to 2 wt% of limestone, 0.1 to 0.5 wt% of salt, 4 to 5 wt% of tallow, 0.1 to 0.2 wt% of choline chloride, DL- 0.2 to 0.4% by weight of methionine, 0.5 to 0.7% by weight of L-lysine-sulfate, 0.03 to 0.12% by weight of threonine, 0.05 to 0.15% by weight of a vitamin mixture, 0.05 to 0.15% by weight of a mineral mixture, 0.1 to 0.3% by weight of Lactovita, 0.0001 to 0.06% by weight of maduramycin and 0.5 to 2% by weight of EP.

In one embodiment of the invention, the animal may be a chicken or a pig.

The present invention provides a method for efficiently extracting and recovering phosphorus in livestock manure as a method for enhancing the recycling efficiency of livestock manure, which is an animal wastes, and an ultrasonic treated phosphoric acid substitute (EP 1) And a phosphoric acid substitute (EP 2) dried or pored at high temperature. When the livestock feed provided by the present invention was actually fed to the livestock, commercial livestock feed substitutes were obtained in comparison with the case of using commercial livestock feeds.

Fig. 1 shows the result of the phosphorus recovery process in the phosphorus water process.
Fig. 2 shows the results of the degree of decrease in moisture content according to the drying method according to the present invention.
Fig. 3 shows the result of showing the type of the substance for each treatment method.
Fig. 4 shows the body weights and organ weights of an experimental rat (Rat).
FIG. 5 is a result of analyzing AST, ALT, BUN, creatinine, phosphorus and calcium in the blood of rats in order to determine whether or not the recovered human body (EP1 & EP2) has animal biotoxicity.
FIG. 6 is a result of examining the tissues (liver, kidney, lung, heart) of rats in order to determine whether or not the recovered human body (EP1 & EP2) has animal bio-toxicity.
FIG. 7 is a photograph showing the growth of broiler chicks fed the feed prepared according to the method of the present invention on a weekly basis.
FIG. 8 shows the results of photographing organs (heart, kidney, liver, cecum, proximal, small intestine, tibia) of the chickens fed with the feed prepared according to the method of the present invention.

The present invention provides a feed composition for livestock containing a phosphorus compound extracted from livestock manure as an active ingredient.

Phosphorus is generally taken from phosphorus and is widely used in feed, fertilizer and chemical industries. Currently, there is a pressing need to increase prices and secure volume as the exhaustion of the factor is deepening.

On the other hand, the biggest problem in livestock breeding is livestock waste disposal such as livestock manure. Environmental pollution caused by stench odor and the cost increase to cope with the pollution have always been persistent. There is no room for improvement. The inventors of the present invention focused on this problem and confirmed that the phosphorus compound recovered from livestock manure can be used as livestock feed while continuing the research to industrially utilize the phosphorus compound recovered from livestock manure.

Accordingly, the present invention provides a feed composition for livestock containing a phosphorus compound extracted from livestock manure as an active ingredient.

The livestock manure of the present invention may be a livestock mixture of bovine, manure, or fennel, or a mixture of various livestock fractions at a predetermined ratio. At this time, it is preferable to use cattle, pigs, and poultry which do not use antibiotics and insecticides.

The 'phosphorus' of the present invention may be selected from a method of drying at a microwave and a high temperature, more preferably a microwave treated at 60 to 100 Hz / g for 1 to 10 minutes .

In order to remove ammonia toxic to an animal from a calcium phosphate replacement material recovered from livestock manure, it is subjected to ultrasonic treatment (microwave treatment) at 60 to 100 Hz / g for 1 to 10 minutes, Preparing a treated phosphate substitute (EP 1); And (b) adding to the phosphate substitute (EP 1) of step (a) the corn, wheat, gluten, corn steep liquor (DDGS), whole soybean, soybean meal, vegetable oil, limestone, salt, Which comprises the step of adding DL-methionine, L-lysine-sulphate, threonine, a vitamin mix, a mineral mix, Clinacox, Lactovita and / or Maduramycin Thereby providing a feed production method.

The 'ultrasound-treated phosphoric acid substitute (EP 1)' of the present invention is a microwave treated for 1 to 10 minutes under a condition of 60 to 100 Hz / 15 to 25% by weight and magnesium of 0.5 to 2% by weight.

The present invention relates to a method for removing ammonia toxic to an animal from a calcium phosphate replacement material recovered from livestock manure by (a) drying at 100 to 600 ° C for 10 minutes to 10 hours to obtain a powdered phosphate substitute (EP 2); And (b) adding to the powdered phosphate substitute (EP 2) of step (a) the corn, wheat, gluten, DDGS, whole soybean, soybean meal, L-lysine-sulphate, threonine, a vitamin mixture, a mineral mixture, Clinacox, Lactovita and / or Maduramycin, The present invention provides a method for producing a feed for livestock.

The 'powdered phosphoric acid substitute (EP 2)' of the present invention is powdered by being dried at a high temperature of 100 to 600 ° C. for 10 minutes to 10 hours, containing 5 to 15% by weight of calcium, 10 to 30% by weight of phosphorus, And 5 to 15% by weight of magnesium.

The present invention also provides a broiler feeding method characterized in that the broiler feed composition is fed to broiler chickens.

Hereinafter, the present invention will be further described by way of examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

≪ Example 1 >

Analysis of phosphorus recovery and operation stability in phosphorus recovery system

The present inventors analyzed the phosphorus recovery rate and the stability of the process in the system by installing an empirical phosphorus recovery device on the farm in order to recover the phosphorus compounds in the pot by intermittently aeration for 60 days.

Sampling for analysis was conducted once a week. The analysis items are shown in Table 1.

As shown in Table 1, the recovery efficiency of phosphorus compounds in the phosphorus compound recovery tank was about 74% based on the soluble compound, and about 43% of the solids was removed together with the crystals. Additionally, NH ₄ -N jeeoyul and TOCs of the process was shown in each of 22%, 47%, and the reason for low NH ₄ -N removal efficiency in the process is NH to form a crystal in response to an OP with the same molar ratio ₄ Since the concentration of -N is relatively higher than that of OP.

Fig. 1 shows the analysis of the components in the whole system and the phosphorus compound recovery apparatus. The phosphorus compound recovery rate was about 20% lower than that of the initial Mg source (1.0M) The recovery rate was 85% or more.

It was found that the amount of phosphorus compound in the solution recovered through the phosphorus compound recovery device was very small and that the amount of Mg source injected for efficient phosphorus compound recovery was about 1.2M based on the soluble compound and 85% Efficiency can be obtained.

The control ratios of OP and TP in Fig. 1 are important indexes for judging that crystallization is occurring in the phosphorus compound recovery device.

< Example  2>

Processing, processing and production of test materials for the animal industry

The present inventors have sought to find efficient, hygienic and safe pulverization methods for the use of raw materials recovered from a phosphorus recovery device as a substitute for phosphorus or calcium phosphate. In order to treat raw materials of phosphorus resources extracted from livestock manure The method and conditions are shown in Table 2.

The microwave and heat treatment methods were selected for the production of test materials for the animal industry and the amount of water loss by treatment method was analyzed to determine the decrease in water content per treatment time. As a result, when the microwave treatment was performed, the moisture content was 17% or less after about 7 minutes And the moisture content was about 6% at 9 hours after the treatment at 105 ° C and decreased to 4.75% after 30 minutes at 550 ° C.

Therefore, the treatment time required for reducing the moisture and ensuring the hygienic properties is 5 to 7 minutes for microwave, 6 hours for heat treatment at 105 ° C, 550 Lt; 0 &gt; C for 25 to 30 minutes (see Fig. 2).

As a result of water content and sensory evaluation, it was found that the initial raw material and the product dried at 105 ℃ had a bad odor. When the microwave was used, there was a little odor. (See Table 3).

In order to use as a substitute for an animal industry, microbial changes were analyzed by treatment method because the death of harmful microorganisms is important. As a result, it was found that when the microwaves were treated or the heat treatment was performed at 550 ° C. for 30 minutes, all the microorganisms in the raw material were killed (see Table 4).

As a result of analysis of total phosphorus content, which is the solubility of each substance by treatment method, the content of soluble compound in the raw material recovered in the initial process was about 118 mg / g (based on DM) and the content of total phosphorus was about 185 mg / g The total phosphorus contents of the raw materials were 226, 206 and 367 mg / g, respectively, when treated with microwave, heat treatment Ⅰ, and heat treatment Ⅱ. (See Table 5).

FIG. 3 shows the shape of the raw material produced in the process by various methods. When the raw material is completely ashed, the color is grayish.

In addition, the present inventors after heat treatment in order to examine whether the phosphate substitute in EP 1 and EP 2 prepared by the method of the present invention in fact is suitable to use as animal industrial calcium phosphate alternative sources, microwave and 550 ^o C, toxicity in the living body (Table 6). It was found that ammonia was easily removed from raw materials recovered from livestock manure by microwave and 550 ^o C heat treatment technique.

< Example  3>

Of human resources recovered from livestock manure In vivo  Confirm stability

The present inventors extracted phosphorus resources from livestock manure by using a phosphorus compound recovery device installed in pig farms in Palmieri, Chuncheon city, Gangwon-do and investigated whether the microwave and heat-treated phosphate substitutes (EP 1 and EP 2) The experimental animals were divided into 5 groups (1 control group, 4 treatment group) after 30 sprague-dawley female rats weighing 200 ± 10g. Experiments were carried out by dividing the mice.

(1 mg / kg, 10 mg / kg) for 1 day in DMSO and 2 mg / kg in 1% DMSO, respectively. (See Table 7) were fed free-form and the light period was maintained at 12-hour intervals.

Twenty-four hours after the final feeding of the test substance, the body weight was measured six times per treatment and anesthetized with diethyl ether. Then, a blood sample was taken from the heart, and blood ALT (aspartate aminotransferase), alanine aminotransferase ), Phosphate, Calcium, Creatinine and BUN (Blood urea nitrogen) were measured. The organs were extracted from liver, lung, kidney and heart, And histopathologic examination was performed.

The contents of the test substances produced for the toxicity evaluation test are as shown in Table 8. The contents of total phosphorus were 221.0 mg / g DM and 259.8 mg / g DM when treated with microwave treatment and heat treatment (550 ℃), respectively.

(Body weight, liver, kidney, lung, and heart) of rats administered orally for 4 weeks, and body weight were not significantly different in all 5 treatments (1 treatment group and 4 treatment groups) <0.05). It was found that the control group (Vehicle) was somewhat higher than the treatment group M (microwave treatment group) and treatment group D (550 ° C. heat treatment group) (see FIG.

There were no significant differences in the organ weights between the groups. No specific findings were observed, and AST, ALT, BUN, Creatinine, Phosphate, Calcium (Fig. 5). As a result, the control and treatment groups showed normal ranges, and the concentration of the analytical items did not show a large difference between them, indicating that there was no toxicity due to the produced substances.

FIG. 6 is a result of examination of the tissues (liver, kidney, lung and heart) of the control and the treatments. Only normal cells were observed in the tissues of each organs, and no particular pathological changes were found.

Therefore, it was found that the phosphorus compound recovered from the animal manure of the present invention did not cause any toxicity to the living body of the animal.

< Example  4>

Identification of Phosphorus Substitution Effect by Animal Application Experiment

Specimens were tested in broiler chickens to evaluate the availability of the factor source recovered from the inventive livestock powder, which was confirmed to be safe through animal biotoxicity assessment, as an alternative calcium phosphate feed.

<4-1> Experiment 1

To investigate whether the factor sources recovered from the inventive livestock feeds are suitable for broiler feed, the present inventors disclosed 525 male broiler chicks (75 ± 10g) of 308 ROSS species, , Microwave treatment group, heat treatment group). The temperature of the breeding room was maintained at 33 ℃ from the day of embryo to 3 days, followed by 2 ~ 3 ℃ per week, and the lighting was lit for 24 hours. The specimens were run on the attached farm for two weeks and stopped at week 3 due to sudden outbreaks of massive deaths.

Table 9 shows the contents and amounts of phosphorus compounds used in the feed formulation. The control group included MDCP (Monodicalcium Phosphate, 21%, Powder / Granular), which is mainly used in poultry feed, and two kinds of test substances Microwave treatment group, heat - treated group) were added and the mixture was divided into two groups.

The content of total phosphorus in the MDCP was 21%, and the content of total phosphorus in the test material was 22.1 and 26.0%, respectively. (See Table 8).

Therefore, experimental feeds were prepared by varying the amount of MDCP and test substance added. The compounding ratios of the experimental feeds and chemical composition are shown in Table 9.

It is known that calcium and phosphorus are closely related to metabolic processes, and ratios of calcium and phosphorus required for chick growth are in the range of 1.0: 1 to 2.5: 1 (3.3: 1).

As a result, the ratio of calcium to phosphorus was calculated by analyzing the chemical composition of the experimental diets. As a result, the mixed calcium phosphate MDCP (control) and the microwave treated factor (treatment M) D) to 2.3: 1 / 2.3: 1, 2.0: 1 / 2.6: 1 and 2.0: 1 / 2.5: 1, respectively, as shown in Table 9.

The first experiment was aborted at the 3rd week due to the sudden onset of the massive death due to the sudden heat, and we were able to investigate only the broiler performance score except the blood test, organ weight, skeletal component and so on. The nutrient intake of the broiler chicks was significantly higher than that of the control (p <0.05). The feed intake was slightly higher in the MDCP (control) than in the control (p <0.05).

The amount of weight gain was the highest in the control group, which had higher feed intake, and showed statistical significance. The feed conversion ratios were 1.25, 1.30, and 1.26 for MDCP (control), microwave treated factor (treated M), and high temperature dried factor (treated D), which are commercially available mixed calcium phosphate. Similar results were obtained without any difference. The results of two weeks showed that the factor sources (microwave treatment, heat treatment and drying at high temperature) prepared by the method of the present invention added to the feeds were similar to calcium phosphate Effect.

However, considering that the breeding period of broiler chickens is usually 30 ~ 35 days, it is difficult to conclude definite conclusions due to lack of research results on the specification period and survey items.

Therefore, the inventors of the present invention conducted experiments for the application of the second broiler system.

<4-2> Experiment 2

In order to examine whether the test substance of the present invention is suitable as a feed for broiler chickens, the present inventors disclosed 202 male numbers of male ROSS 308 male (80 ± 5) male, 3 treatment (MDCP, microwave treatment group, The temperature of the breeding room was kept at 33 ℃ for 3 days from the embryo day, then decreased by 2 ~ 3 ℃ per week and maintained at 25 ℃ from 22 days. Illumination was carried out 24 hours a day, and feed and water were fed free.

To supplement the results of Experiment 1, specimens were tested for 5 weeks (1 ~ 2 weeks / 3 ~ 5 weeks).

The preparation and specification of the experimental diets were carried out in the same manner as in Experiment 1 for broiler performance, and FIG. 8 shows growth of broiler chickens for 5 weeks during the experiment. It is generally known that lack of phosphorus or lack of availability in feed usually reduces appetite and weakens it, kills within 10 to 12 days, and if it is somewhat deficient, it causes osteomalacia or poor growth. Careful observation of the broiler chickens during the specimen period revealed no abnormalities in the outline and general symptoms of both the control and treatment groups (see FIG. 7).

The test results were analyzed for 4 weeks and the results are shown in Table 11.

Feed intake was 659.6, 715.6, and 692.7g in MDCP (control), microwave treated factor (treated M), and high temperature dried factor (treated D), respectively, which are commercially available mixed calcium phosphate. There was no significant difference (p <0.05), but there was no significant trend or significant difference in weight gain or feed conversion ratio (Table 12).

Statistical analysis of feed intake, body weight gain, and feed conversion ratio showed no correlation between control and treatments (p <0.05).

As a result of the 4-week specimen results, the factor source (microwave treatment, drying at high temperature) prepared by the method of the present invention added to the feed was judged to have similar effects to calcium phosphate used in general, and calcium phosphate (MDCP) Table 12 shows the nutrient digestibility of the broiler when the metabolic test was conducted to determine the effect of the test substance on the productivity of broiler chickens.

The nutrient digestibility of the experimental diets was not significantly different between dry matter and crude protein and the digestibility of Crude fat and Crude ash was higher than that of control (See Table 12).

In addition, the present inventors extracted the heart, kidney, liver, cecum, proximal, small intestine and tibia as shown in FIG. 8 and analyzed the effect of the test substance on the digestive organs to measure the weight and length of the organ. And no abnormalities were observed in both of the treatments.

The length of the organs (heart, kidney, liver, cecum, proximal, small intestine, tibia) was expressed as a percentage of body weight and the length (small intestine, tibia) , The ratio of organs to body weight was generally lower in treatment group D but there was no significant difference and it was found that there was no particular tendency in comparison with organ groups (see Table 13) ).

The liver is known to be an organ which is synthesized in the body or converts various lipophilic substances introduced from the outside into water-soluble substances and discharges through the bile or urine. (M) treated with mixed micronized calcium phosphate (control), microwave treated factor (treated M), and high temperature dried factor (treated D) were 3.14, 3.46 and 3.04%, respectively. , Control, and treatment D, but there was no significant difference.

To analyze the effect of the test substance on the skeletal development of the broiler chick, the tibia of the right leg was taken and analyzed for constituents such as dry matter, crude ash, calcium and phosphorus As a result, there was no significant difference between control and treatments (see Table 14).

Calcium and phosphorus contents of the tibia were affected by the levels of phosphorus in the feed, which was higher in the order of control, treatment group A and treatment group M. In addition, blood samples were collected from the heart to determine the physiological effects of using the test substance as a MDCP replacement agent. As a result, there was a significant difference only in calcium, and in the other items (AST, ALT, BUN, Creatinine and Phosphate) (See Table 15).

The chemical composition of late diets was 0.64 and 0.64 (p <0.05) for the mixed calcium phosphate MDCP (control) and microwave (factor M) and high temperature factor , And 0.63%, respectively, and blood test showed significant difference in microwave treatment compared to control. AST and ALT were reported to be elevated when they were injured in the liver including kidneys. However, there was no damage to the body organs due to the test substance. RBC, WBC, NEU, LYM and EOS also showed significant differences It did not appear. WBC, NEU, LYM, and EOS have been reported to increase the level of inflammation, and there were no abnormal symptoms when the control and treatments were compared.

In addition, when analyzing the broiler performance, weight and length of the organs, and blood test results, the factor source (microwave treatment, drying at high temperature) prepared by the method of the present invention added to the control and feed using normal calcium phosphate was replaced with an alternative zero There is no significant difference between the treatments used.

In the present invention, it has been found that human resources can be extracted from livestock manure and produced as an animal feed, and it is characterized in that an optimized blending ratio is determined for producing a broiler feed. In addition, the feed prepared by the method of the present invention is not only toxic to animals but also nutritionally superior to commercially available calcium phosphate feed. Therefore, using livestock manure, which is difficult to treat, is very economical in that it is environmentally friendly as well as has developed a material that can replace phosphorus stones that depend on total imports.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (9)

delete delete delete (a) preparing an ultrasonic treated phosphate substitute (EP 1) by microwave treatment at 60 to 100 Hz for 1 to 10 minutes; And
(b) In step (a), the phosphoric acid substitute (EP 1) is mixed with a mixture of corn, wheat, gluten, cornstarch, DDGS, whole soybean, soybean meal, A method for manufacturing a feed for livestock comprising the steps of adding DL-methionine, L-lysine-sulphate, threonine, a vitamin mixture, a mineral mixture, Clinacox, Lactovita and Maduramycin Characterized in that the said eutectic treated phosphate substitute (EP 1) contains from 5 to 15% by weight of calcium, from 10 to 25% by weight of phosphorus and from 0.5 to 2% by weight of magnesium. delete (a) drying at 100 to 600 ° C for 10 minutes to 10 hours to prepare a powdered phosphate substitute (EP 2); And
(b) adding to the powdered phosphate substitute (EP 2) of step (a) the corn, wheat, gluten, corn steep liquor, DDGS, whole soybean, soybean meal, Which comprises the step of adding choline chloride, DL-methionine, L-lysine-sulphate, threonine, vitamin mix, mineral mix, Clinacox, Lactovita and Maduramycin Wherein the dried and powdered phosphate substitute (EP 2) contains 5 to 15% by weight of calcium, 10 to 30% by weight of phosphorus and 5 to 15% by weight of magnesium. delete The method according to claim 4 or 6,
35 to 40 wt% of corn, 10 to 20 wt% of wheat, 1 to 3 wt% of gluten, 2 to 6 wt% of DDGS, 6 to 10 wt% of whole soybean, 17 to 21 wt of soybean meal 1 to 3% by weight of limestone, 1 to 2% by weight of limestone, 0.1 to 0.5% by weight of salt, 4 to 5% by weight of woji, 0.1 to 0.2% by weight of choline chloride, 0.2 to 0.4% by weight of DL- Lactobita, Lactobita, Lactobita, and Lactobita have been found to be useful in the treatment of diabetic nephropathy, 0.1 to 0.3% by weight, Maduramycin 0.0001 to 0.06% by weight and EP 0.5 to 2% by weight. The method according to claim 4 or 6,
Wherein the livestock is a chicken or a pig.

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