KR102475512B1 - Method for Stimulating Maturation of Cardina denticulata - Google Patents

Abstract

Provided is a method for inducing maturation of Caridina denticulata, comprising: a freshwater shrimp capturing step (a) of capturing freshwater shrimps in a river; an input preparation step (b) of setting the temperature of breeding water in a sorting tank to be the same as the water temperature at the time of capturing the freshwater shrimps in the step (a) and installing a Caridina denticulata separation device floatable on the water surface of the sorting tank; a separation and breeding step (c) of breeding the freshwater shrimps for a set period after inputting the freshwater shrimps captured in the step (a) in the sorting tank prepared in the step (b) to separate the Caridina denticulata from other freshwater shrimps; a Caridina denticulata separation step (d) of, after the step (c), sorting and capturing the Caridina denticulata clustered on the inner wall of the Caridina denticulata separation device and the sorting tank; an egg-brooding inducement step (e) of inputting the Caridina denticulata sorted in the step (d) into a breeding tank in which the temperature is made to be the same as the water temperature in the sorting tank, then increasing the water temperature in the breeding tank at the rate of 1-2 ℃/day, and then keeping the rate until ecdysis occurs at 10-15 ℃; and a fry incubation step (f) of confirming mass ecdysis in the breeding tank in step (e), raising the breeding water temperature at a rate of 1 to 2℃/day to maintain the temperature at 20 to 25℃ and to check the eggs, and moving and inputting the eggs into a main breeding tank to incubate fry, thereby in the case of sorting, inducing maturation of, and breeding the Caridina denticulata, selectively and easily sorting the Caridina denticulata from the captured freshwater shrimps and improving survival rate and egg-brooding rate of the Caridina denticulata to enhance the incubation amount of the initial fry of the present breeding method.

Description

Method for inducing maturation of a new squirrel {Method for Stimulating Maturation of Cardina denticulata}

The present invention relates to a method for inducing maturation of a saber ( Cardina denticulata denticulata De Haan ), and more particularly, by effectively selecting a single variety of a saber from a variety of first-caught freshwater shrimps that are treated as landslides in nature, and The present invention relates to a method for inducing maturation of a larvae, in which a single species of larvae can be stocked during main breeding, as well as an increase in the hatching rate of postlarvae by increasing the survival rate and incubation rate.

It is a collective term for freshwater shrimp, such as Palaemon paucidens De Haan, scavenger ( Cardina denticulata denticulata De Haan), and raw fish ( Paratya compressa ), which are not a single specific breed that is generally vomited. get caught Usually, one type of raw fish is known as Toha, and it is comprehensively expressed as raw shrimp by confusing saebangi and raw fish, which have a similar appearance, but this is an incorrect expression.

Toha varieties that have domestic industrial value and are distributed are mostly line shrimp and saebaengi. Among the two varieties, the variety that is grafted into the aquaculture business for mass production and processed and distributed as salted seafood is Saebangi. In addition, recently, it is actively used to improve the environment for breeding ornamental fish, such as decomposition of moss and solid substances in the aquarium, and it is introduced as a new ornamental breed by improving and selecting mutants with various colors.

Saebangi is a freshwater shrimp with a body length of about 25 mm. In terms of appearance, the body color is dark brown, and there is a spine-shaped mottled pattern on the middle line of the upper surface. The carapace has an upper antennal spine and no upper eye spine, and the anterior lateral edge of the carapace is spine-shaped. There are 10 to 20 teeth on the upper edge of the rostrum and 1 to 9 on the lower edge. First and second pectoral legs have pincers. Pincers are mainly used for prey rather than attacking or defending other individuals. However, it is difficult to observe these characteristics when collected as a very small species, and it can be confirmed through detailed observations such as glass water tanks and beakers.

Most of the inland aquaculture varieties used industrially have low public acceptance due to their unique scent and foreign body sensation, but Saebaengi is widely recognized as a subsidiary material rather than a single use recipe, so it has a very high preference and can be linked with various industries.

However, due to changes in the habitat environment, the distribution area and population of the new snapper are greatly reduced, which limits the ability to meet the demand for catches, and when switching to a rearing fishery, there is a difficulty in supplying mother hatchlings. situation.

When collecting normal mother saebaengi, there is a limit to direct selection by breed after catch because the habitat environment of other species handled, especially row shrimp, and breed overlaps. In addition, in the case of mixed farming with shrimp, it is the main cause of the reduction of the maximum production per unit area due to the formation of areas between the shrimp and the shrimp family, which intensifies aggression and causes predation at the hatching stage during the molting of the shrimp.

Therefore, selection technology is essential before breeding, and it is difficult to predict the hatching amount under PL because the amount of eggs between individuals is around 50 to 150 at maturity, so it is impossible to plan farming with a small number of broodstock. Therefore, it is essential to develop a technology that increases the rate of hatching by inducing mass maturation of female hatchlings of randomly captured sex through artificial stimulation to increase the rate of incubation.

Accordingly, Korean Patent No. 10-1845608 discloses a method for mass production of artificial seeds in soil, and Korean Patent No. 10-2323857 discloses a method for promoting and inducing sexual maturation of crustaceans using red LEDs. , The configuration of the method for inducing maturation of the saber, which can select the species of the mother hatchet and increase the incubation rate of the present invention, is not posted, showing a difference.

Domestic Patent No. 10-1845608 discloses the step of manufacturing a cage for artificial seedlings that can separately accommodate mother and postlarvae in the mother and postlarva compartments (1), natural food for artificial seedlings by fermenting grains step (2) of producing, collecting broodstock, receiving the broodstock in the cage for artificial seedlings in step (1), and feeding natural food in step (2) (3), hatching postlarvae Mass production of artificial seedlings characterized by comprising the step (4) of breeding PLs by feeding natural food in step (2) and the step (5) of harvesting PLs of step (4) when accommodated in the compartment. By providing a method, a method for mass-producing artificial seedlings in the soil that can perform stable aquaculture by producing a large amount of healthy sub-larvae by improving the low spawning amount and severe formula of the same species is disclosed. Korean Patent Registration No. 10-2323857 discloses a method for promoting and inducing sexual maturation of crustaceans using red LEDs, including the step of radiating red LEDs to promote sexual maturation of crustaceans, wherein the sexual maturation is carried out by the formation of crustacean blood sugar raising hormone ( A method for accelerating and inducing sexual maturation of crustaceans using a red LED in which secretion of CHH) is promoted or secretion of yolk-inhibiting hormone (VIH) is inhibited. Domestic Patent No. 10-1443851 discloses that when shrimp larvae are collected and reared, hatching of mother shrimp can be promoted to increase the number of larvae for seed production, and external growth and internal components of the larvae can be strengthened. Disclosed is a production system and production method of shrimp seeds that can be effectively used in the shrimp farming industry because it is possible to secure seeds with excellent quality and increase the survival rate. Korean Registered Patent No. 10-1711740 screens and mass-cultivates microorganisms in Korean-style festival farms and quantitatively injects them into farms to measure and prepare the water quality environment in real time so that the bacterial concentration in the farms can be maintained constant. It discloses a management system for a festival shrimp farm that overcomes the effects of daily temperature difference, temperature difference, seasonal change, and rainfall.

In order to solve the above problem that it is difficult to select only the larvae among the captured parental species, the present invention effectively selects only a single species of larvae from the captured larvae, increases the survival rate and the nesting rate of the larvae, and improves the first hatching of postlarvae during the breeding process. The purpose is to provide a maturation induction breeding method that can be.

A method for inducing maturation of Saebangi according to an embodiment of the present invention includes the step (A) of catching land and rivers in a river; A stocking preparation step (b) of setting the breeding water temperature in the sorting tank to be the same as the water temperature at the time of catching land and downstream in step (a), and installing a separation device for blackfish that can float on the surface of the sorting tank; After the land streams caught in step (a) are stocked in the sorting tank prepared in step (b), the water temperature in the sorting tank is lowered at a rate of 1 to 2 ° C / day and maintained at 1 to 5 ° C for 10 to 20 days Isolation and breeding step (c); After the step (c), a blackbird sorting step (d) of selecting and capturing the blackbirds clustered on the inner wall of the blackbird separator and the sorting tank; After stocking the blackbirds selected in the step (D) in a breeding tank made with the same water temperature as the sorting tank, the water temperature in the breeding tank is raised at a rate of 1-2℃/day, and then kept at 10-15℃ until they are molted. Incubation induction step (E) to maintain; After confirming the mass ecdysis in the breeding tank in step (e), raise the breeding water temperature at a rate of 1 to 2 ° C / day and maintain it at 20 to 25 ° C to check the eggs, move to the main breeding tank, and stock PLs It may consist of a sublarval hatching step (bar) to hatch. It may be formed by mixing a blue food coloring in the breeding tank breeding water of the egg induction step.

According to another embodiment of the present invention, a method for inducing maturation of Saebangi includes the step of catching land and rivers in a river (A); A stocking preparation step (b) of setting the breeding water temperature in the sorting tank to be the same as the water temperature at the time of catching land and downstream in the step (a), and installing a separation device for blackfish that can float on the surface of the sorting tank; Separation and breeding step of separating the land and subsea caught in step (a) by breeding them for a certain period of time after stocking them in a sorting tank prepared in step (b); After the step (c), a blackbird sorting step (d) of selecting and capturing the blackbirds clustered on the inner wall of the blackbird separator and the sorting tank; Incubation induction step (E) of stocking the saberfish selected in step (D) in a breeding tank prepared at the same water temperature as the selection tank and rearing them until they are molted; It may be composed of a sublarval incubation step (f) of confirming mass ecdysis in the breeding tank in step (e), confirming the eggs, and transferring them to the main breeding tank to incubate the sublarvae.

The saber separator is a tubular shape with openings at both ends and installed perpendicularly to the bottom of the tank, and the oxygen transfer tube opening has a narrow indentation toward the opening of the oxygen transfer tube and forms a bend toward the opening The collection frame may be installed through and an oxygen generating device may be installed to extend inside the oxygen transfer pipe.

In the separation and breeding step (c), the water temperature in the sorting tank is lowered at a rate of 1 to 2 ° C / day after the stocking of the land stream and maintained at 1 to 5 ° C for 10 to 20 days. After raising the water temperature at a rate of 1 ~ 2 ℃ / day, the water temperature is maintained at 10 ~ 15 ℃ until shedding, and after confirming the large amount of shedding in step (f), the breeding water temperature is 1 ~ 2 ℃ / day It may be to rise at a rate of 20 ~ 25 ℃ to maintain until the confirmation of incubation.

The present invention has the advantage of being able to selectively and easily select new beetles from captured land streams when selecting and maturation inducing breeding of new beetles, and improving the hatching capacity of the first postlarvae of this breeding culture by increasing the survival rate and nesting rate of new beetles.

1 shows a schematic diagram of a method for inducing maturation of a saber of the present invention.
Figure 2 shows the saebang separation device of the present invention.
Figure 3 shows a schematic diagram of the operation of the saebang separator of the present invention.
Figure 4 shows a perspective view of the sorting tank in which the saberfish separator of the present invention is installed.
Figure 5 shows an embodiment of the saebang separation device of the present invention.
Figure 6 shows a schematic diagram in the sorting tank of prawns and prawns before and after sorting according to the present invention.
Figure 7 shows the number of breeding tanks in the incubation step of the present invention.
Figure 8 shows the molting of the hatching stage of the present invention.
Figure 9 shows the eggs in the hatching stage of the present invention.
Figure 10 shows the separated shrimp (A) and shrimp (B) individuals according to Experimental Example 1 of the present invention.

Hereinafter, a detailed explanation will be given with reference to drawings related to the maturation inducing method of the present invention.

1 shows a schematic diagram of a method for inducing maturation of a saber of the present invention. The method for inducing maturation of Saebangi of the present invention includes the step (A) of capturing soil and submerged soil in a river; A stocking preparation step (b) of setting the breeding water temperature in the sorting tank to be the same as the water temperature at the time of catching land and downstream in the step (a), and installing a separation device for blackfish that can float on the surface of the sorting tank; Separation and breeding step (c) of breeding the land streams caught in step (a) in the sorting tank prepared in step (b) and breeding them for a certain period of time to separate the blackfish from other land streams; After the step (c), a blackbird sorting step (d) of selecting and capturing the blackbirds clustered on the inner wall of the blackbird separator and the sorting tank; After stocking the blackbirds selected in the step (D) in a breeding tank made at the same water temperature as the sorting tank, the water temperature in the breeding tank is raised at a rate of 1-2℃/day, and then kept at 10-15℃ until they are molted. Incubation induction step (E) to maintain; After confirming the mass ecdysis in the breeding tank in step (e), raise the breeding water temperature at a rate of 1 to 2 ° C / day and maintain it at 20 to 25 ° C to check the eggs, and transfer to the main breeding tank to incubate postlarvae It consists of a sublarval hatching step (bar) to hatch.

(A) Land and downstream capture stage

The earth and river catch step (a) of the present invention includes the step of catching earth and river by fishing activities in rivers and lakes. In the wild land and submersibles captured in the step of the present invention, string shrimp and prawns other than sabers may be mixed.

(B) Preparing for stocking

The stocking preparation step (b) of the present invention includes the step of setting the water temperature of the breeding water stored in the sorting tank to be the same as the water surface captured by the land stream, and installing a saberfish separation device that can float on the surface of the breeding water in the sorting tank.

The palaemon paucidens referred to in the present invention is a species of the genus Palaemon belonging to the family Shrimpidae, and lives in various areas with a wide range of environmental changes from freshwater areas to estuaries and marine areas, and about 13 species are known to be distributed in Korea. have. With the same habits as prawns, when they are introduced into breeding facilities, they need food organisms in the early stages, so they prey on larvae instead of water fleas, and then eat the larvae that continuously shed during the growth process, making it difficult to increase the population of the prawns.

Therefore, when harvesting broodstock for aquaculture, it is essential to sort out the two breeds whose habitats overlap, resulting in mixing. In the past, this was done manually, but it was difficult to distinguish the two species by appearance, as well as the disadvantages of requiring time and effort. Prior to harvesting and managing broodstock, screening of prawns and prawns should be carried out.

Fig. 2 shows the shrimp separator of the present invention, Fig. 3 shows a schematic view of the operation of the shrimp separator of the present invention, and Fig. 4 shows a perspective view of the sorting water tank in which the shrimp separator of the present invention is installed.

The shrimp separator of the present invention has an oxygen transfer tube 100 installed perpendicularly to the bottom of the water tank in a tubular shape with both ends open, and a collection frame having a cross section forming a hemisphere so as to pass through the openings at both ends of the oxygen transfer tube and converging toward the transfer tube ( 200), and an oxygen generator 300 may be installed to extend inside the oxygen transfer pipe.

The oxygen transfer pipe 100 according to the present invention has a hollow tube shape, and the oxygen generator is installed to extend into the lower space inside the oxygen transfer tube. The oxygen generator can supply compressed air and can be preferably implemented as an air stone, but it is not limited thereto as long as the breeding water can move upward through the oxygen transfer pipe along with oxygen discharge. The oxygen transfer pipe is installed perpendicularly to the bottom of the tank, and one or more moving holes 110 are formed on the lower side thereof, so that the new fish or breeding water moves into the moving pipe through the moving hole and rises along with the operation of the oxygen generator.

The collection frame 200 has a structure in which both ends are open and a depression portion narrowing toward the opening of the oxygen transport tube is formed, and one or more through holes are formed on the side surface, and the central portion is installed to pass through the openings at both ends of the oxygen transport tube. The collection frame may preferably form a hemisphere in cross-sectional structure, but is not limited thereto as long as it forms a curved portion that narrows in width toward the oxygen transfer pipe.

The collection frame includes an upper collection frame installed through the upper opening of the oxygen transport tube and a lower collection frame installed through the lower opening of the oxygen transport tube. An escape prevention net 210 is installed on the side of the upper collection frame facing the opening of the oxygen transfer pipe. The escape prevention net makes it easier to catch the shrimp in the sorting step to be described later by preventing the shrimp from escaping to the outside of the shrimp separator when the breeding water moves with the shrimp to the top of the oxygen transfer pipe due to the operation of the oxygen generator.

In the shrimp separator of the present invention, an oxygen transfer pipe is installed perpendicular to the bottom of the sorting tank in which breeding water is stored, and when oxygen (gas) is generated from the oxygen generator installed in the pipe, the mixture of oxygen and breeding water is lighter than the breeding water outside the pipe. The difference in specific gravity increases the breeding number in the tube.

In general, newbies have a habit of gathering at a low temperature similar to the winter season or swimming and distributing to places where water flows, so as shown in FIG. will be captured in

At this time, the prawns and prawns that are caught together and stocked in the sorting tank do not cluster, form an area, and are distributed in the bottom of the tank (bottom of the tank), so that they can be easily separated from the shrimp. In addition, when selecting and capturing shrimps clustered on the lower side of the wall of the sorting tank in the shrimp selection step to be described later, prawns clustered on the bottom surface of the sorting tank may be mixed. The shrimp separator according to the present invention In the case of installation, by being crowded on the bottom surface of the floating body, the mixing of the shrimp can be prevented by being spatially separated from the prawns clustered on the bottom surface of the sorting tank.

Figure 5 shows a cross-sectional view of an embodiment of the shrimp separator of the present invention. The saboteur separator according to an embodiment of the present invention has a structure in which the lower end is spaced apart from the bottom of the tank at a predetermined interval and the upper end can float on the surface of the sorting tank, and is installed vertically with the tank in a tubular shape with openings at both ends. The oxygen transfer tube 100 and the openings at both ends of the oxygen transfer tube form a hemisphere in cross section and a collection frame 200 whose width converges toward the transfer tube is installed, and an oxygen generator 300 is installed so as to extend inside the oxygen transfer tube It can be done.

One or more buoys (not shown) are installed on the upper collection frame according to the embodiment, so that the upper part of the shrimp separator of the present invention floats on the surface of the breeding water, and the frame below the upper collection frame can be installed so that it can infiltrate the breeding water. do.

The new fly separator according to an embodiment of the present invention is spaced apart from the bottom of the tank at a certain distance and additionally installed with a buoy at the top so that it can float inside the sorting tank, thereby increasing the separation efficiency by clustering the new fly distributed throughout the sorting tank. can

(C) Isolation and Breeding Stage

In the separation and breeding step (c) of the present invention, the land and submerged fish caught in step (a) are stocked in a sorting tank prepared in step (b), and then the water temperature in the sorting tank is lowered at a rate of 1 to 2 ° C / day. This is the step of creating an artificial wintering environment by maintaining the temperature at 1-5℃.

When the water temperature of the sorting tank is maintained at 1 to 5 ° C, the prawns and prawns do not cluster in a benthic state with reduced vitality and form an area, whereas the same variety is sorted with the same variety in the upper part or bends such as the wall of the tank. It exhibits a characteristic of clustering in a dark place, so it is possible to easily sort out the purebred birds in the selection tank and breed them separately.

If the breeding water temperature is maintained below 1℃, mass mortality may occur due to the formation of ice crystals. There is a problem of low selectivity.

It is desirable to select when the water temperature of the sorting tank is maintained at 1 to 5 ° C for 10 to 20 days to form a colony of shrimp, which is maintained for less than 10 days. There is a problem that the selection rate is not good, such as being clustered in large numbers or having difficulties in sorting out the small size of the colony.

In addition, when maintained for a long period of more than 20 days and longer than 40 days, the colony of saberfish is maintained, but there is a problem in that economic efficiency is lowered. it is appropriate

In the stage of the present invention, it is possible to help form a colony by maximizing the habit of seeking hiding of the nocturnal saberfish by allowing appropriate natural light to come in without the need to create a complete blackout in the tank.

Feed supply can be minimized, or if the separation step is performed only once, feed supply can be omitted, or if a lot of prawns or prawns are mixed in the soil and subsea, prawns or prawns with high animal protein requirements in the sorting tank Shrimp can be fed to minimize saboteur predation.

(D) Selection step for newbies

While common sabers have the characteristics of clustering at 4 ° C or lower, which is similar to the winter season, shrimp require a benthic area of at least 4 × 4 cm (16 cm 2) or more per individual due to the presence of areas between individuals. At this time, since 1 m2 = 10,000 cm2, it is possible to breed 600 to 625 individuals/m2 (average around 0.66 g, 400 to 500 g/m2 production), which makes it less economical than saberfish.

The step (d) of sorting the sabers of the present invention may include a step of collecting the sacks collected on the sacks separating device and the wall surface after the above step (c). Figure 5 shows a schematic diagram in the sorting tank of prawns and prawns before and after sorting according to the present invention. As shown in FIG. 5, before the sorting, the prawns and prawns sporadically distributed in the sorting tank are distributed in clusters on the wall surface of the tank and the prawn separator after the sorting, while the prawns are sporadically distributed in the benthic. It has the advantage of easy selection.

In addition, in the screening of new birds of the present invention, the selection rate of new birds can be increased by repeating step (c) after selecting new birds once to carry out the second separation and breeding step and the second nest selection step.

According to an embodiment of the present invention, after the first separation and breeding step, the saberfish selected in the first selection step are stocked in another selection tank and the second separation and breeding step is performed. The second separation and breeding step is maintained for 5 to 10 days at the same water temperature of 1 to 5 ℃ as the first. Afterwards, the clustered newbies can increase the selection rate of pure newbies by carrying out the second screening step. The screening step can be performed twice or more, such as the first screening, the second screening, etc., and is more effective in selecting pure saberfish.

That is, the sorting step includes the first sorting step of maintaining the water temperature in the sorting tank at 1 to 5°C for 10 to 20 days to selectively capture the blackbirds with strong gregariousness among the land streams, and the selectively captured blackbirds at 1 to 5°C. It is possible to increase the selection rate of new blackbirds by holding them for 5 to 10 days after stocking them in another sorting tank maintained as a second sorting step to selectively capture new blackbirds with strong gregariousness.

When the selection method according to the present invention is carried out and maintained at 1 to 5 ° C for 10 to 20 days to selectively capture sacrificial larvae in the soil and downstream, the selection rate of larvae standing in the sorting tank is generally very high, at 70 to 75% or more.

(E) Incubation stage

In the incubation induction step (E) of the present invention, after stocking the saberfish selected in step (D) in a breeding tank set to the same water temperature as the sorting tank, raising the water temperature of the breeding water at a rate of 1 to 2 ° C / day , and maintaining at 10 to 15 ° C until peeling.

It is preferable to keep the water temperature of the breeding tank for breeding the selected sabers at the same level as the water temperature of the sorting tank, considering the characteristics of cold-blooded animals, since there is a risk of death due to shock if the water temperature difference between the water temperature of the sorting tank and the water temperature is large. .

In addition, as shown in FIG. 6, the breeding water of the breeding tank according to the present invention is preferably mixed with a blue food coloring to form a darker breeding water. Birds prefer a dark place, and when the environment is created through normal lighting to increase the incubation rate by creating a dark environment, the stress of the cockroach increases when lit. When the blue food coloring is mixed with the breeding water, By providing the same environment from breeding to hatching as well as creating an environment, it is possible to reduce stress as well as increase the incubation rate.

From the water temperature of the maturation induction breeding tank above 10 ° C., feed of 2-3% of the weight of the stocking fish is supplied three times a week. As the feed, the feed quality such as the content ratio of crude protein is not greatly limited, and it is effective to supply a 2-5 mm sedimentary EP (Extruded pellet) feed.

(F) Sublarval hatching stage

In the sublarval incubation step (f) of the present invention, after confirming mass ecdysis in the breeding tank in step (e), raise the breeding water temperature at a rate of 1 to 2 ° C / day and maintain it at 20 to 25 ° C to check eggs, It includes the step of hatching PLs by transferring them to the main breeding tank.

Figure 7 shows the molting of the hatching stage of the present invention. Usually, saberfish dissociate at a water temperature of 10 to 15 ° C, and active swimming is observed in the opposite direction of the flow speed, and mass moulting is observed on the surface of breeding water due to preparation of eggs by mating. The greater the amount of shedding, the greater the amount of initial PL can be secured. It is possible to incubate 50 to 150 grains per mother brood, and if you calculate the average of 100 grains/individual, it is possible to calculate the first hatching amount before main breeding.

Figure 8 shows the incubation individual in the sublarval hatching stage of the present invention. After inducing mating and incubation through observation of the mass molting of the birds, the breeding tank is continuously raised at the same rate of 1 to 2 ° C / day, and then maintained at 20 to 20 ° C to determine whether the fertilized eggs are mature ( Light gray → dark brown) and move to this breeding tank.

Hereinafter, an experimental example according to the maturation induction method of the present invention will be described in detail.

<Experimental Example 1> Effect according to the maturation inducing method of the present invention

According to Experimental Example 1 of the present invention, 6 kg of prawns and 4 kg of prawns captured and selected from a river in Gochang-gun, Jeollabuk-do were stocked in a Φ4.8 m circular tank with a water temperature of 20 ° C. At this time, a 2 kW electric heater was installed in the circular water tank, and blackfish separators were installed at three locations.

After stocking prawns and prawns in a circular tank, feed was supplied at 5% of body weight/day for 20 days. For the feed, a feed exclusively for prawns was used, which was composed of 8% moisture, 50% crude protein, 7% crude fat, 24% carbohydrates (including crude fiber, etc.) and 11% ash.

The water temperature in the circular water tank was lowered to 4℃ at a rate of 1~2℃/day, which took 20 days. After maintaining the water temperature at 4 ° C. for 15 days, individuals clustered at the bottom of the circular water tank were selectively captured.

Figure 9 shows the separated shrimp (A) and shrimp (B) individuals according to Experimental Example 1 of the present invention. As shown in FIG. 9, during the sorting step, the shrimp are clustered and distributed on the tank wall and the shrimp separator, and the shrimp are distributed on the bottom of the tank, making it easy to separate. As a result of visually judging the selection rate of blackbirds among the captured individuals, the selection rate of blackbirds was estimated to be approximately 95%.

After stocking the selectively captured saberfish in another circular tank maintained at a water temperature of 4 ° C, the temperature was raised to 15 ° C at a rate of 1 to 2 ° C / day (takes 15 days), maintained for 15 days, and mass molted individuals were identified. . At this time, prawn-only feed was supplied at 1%/day of fish body weight.

The water temperature of the circular water tank was raised to 20 ° C at a rate of 1 to 2 ° C / day (it took 5 days). At this time, as a result of visual inspection, the nesting individuals of the new beetle occupied approximately 1/3 of all the new beetles. And the water temperature was maintained at 20 ℃ for 20 days, and the final 5.0 kg (around 0.03 g of sublarval weight) was caught. After that, it was observed until day 100, and after 100 days, 14.2 kg of sage was finally caught.

<Experimental Example 2> Effect according to the maturation inducing method of the present invention

In Experimental Example 2 of the present invention, after the first separation step and the first selection step, the second separation step and the second selection step were performed to confirm the maturation induction effect. In the same way as in Experimental Example 1, 6 kg of prawns and 4 kg of wild shrimps captured and selected from a river in Gochang-gun, Jeollabuk-do were stocked in a circular water tank with a water temperature of 20 ° C.

The water temperature in the circular water tank was lowered at the same rate of 1 to 2 °C/day to 4 °C, which took 20 days. After maintaining the water temperature at 4° C. for 15 days, the individuals clustered at the bottom of the circular water tank were first screened and captured.

The weight of the individual captured by the first selection was 4.5 kg. The individuals captured by the first selection were stocked in a circular tank maintained at 4° C. and maintained for 15 days, and the individuals clustered at the bottom of the circular tank were captured by the second selection. The weight of the individuals captured in the second selection was 4.4 kg. As a result of visually judging the selection rate of blackbirds among the individuals captured by secondary selection, the selection rate of blackbirds was estimated to be approximately 98%.

After stocking the selectively captured saberfish in another circular tank maintained at a water temperature of 4°C, the temperature was raised to 15°C at a rate of 1 to 2°C/day (takes 15 days), maintained for 15 days, and mass-molded individuals were identified. did At this time, prawn-only feed was supplied at 1%/day of fish body weight.

Next, the water temperature in the above circular water tank was raised to 20 ° C at a rate of 1 to 2 ° C / day (it took 5 days), maintained for 20 days, and finally 5.2 kg (around 0.03 g of sublard weight) was caught. . After that, it was observed up to 100 days, and after 100 days, 16.5 kg of the new bream was finally caught.

<Experimental Example 3> Maturation induction effect according to the existing method

In Experimental Example 3, the selection rate was confirmed by screening and capturing with the naked eye, which is an existing method. As in Experimental Example 3, 6kg of prawns and 4kg of shrimps captured and selected from the river in Gochang-gun, Jeollabuk-do were stocked in a circular water tank with a water temperature of 20 ° C. Then, among the land streams stocked in the circular tank, saberfish were visually selected and captured. As a result of visually judging the selection rate of blackbirds among the captured individuals, the selection rate of blackbirds was estimated to be approximately 60%.

Individuals visually selected and captured from the circular tank were stocked in another circular tank, lowered to 15 ° C at a rate of 1 ~ 2 ° C / day, and maintained for 15 days. .

Next, the water temperature in the above circular water tank was raised to 20 ° C at a rate of 1 to 2 ° C / day (it took 5 days) and maintained for 20 days. caught a lobster. After that, it was observed up to 100 days, and after 100 days, 4.1 kg of prawns and 5.7 kg of prawns were finally caught.

After the implementation of Experimental Examples 1 to 3, the selection rate according to Experimental Example 3, in which the blackfish was visually screened from the vomit stream, was as low as 60%, while the selection rate was very high as 95% in Experimental Example 1 and 98% in Experimental Example 2. has been measured In addition, the population of the finally captured saberfish was only 4.1 kg in the case of the comparative group, but it was measured as very high as 14.5 kg in Experiment 1 and 16.5 kg in Experiment 2.

The present invention catches land streams prior to breeding and solves the problem of difficulty in planning farming due to the small number of broodstock individuals due to the inclusion of prawns, etc. By providing this possible technology, it has industrial applicability as it contributes to the development of the shrimp aquaculture industry.

100: oxygen transfer pipe 200 collection frame
300: Oxygen generator 210: Departure prevention net
110: moving ball
A: Shrimp B: Shrimp

Claims (3)

Breeding step (1) of breeding for a certain period of time after stocking the caught soil and submerged fish in a sorting tank at the time of fishing;
a step (2) of selecting and catching new birds after the step (1);
A method for inducing maturation of sabers, characterized in that it comprises a; brood induction step (3) of inducing nesting by stocking the sabers selected in step (2) in a breeding tank containing breeding water prepared by mixing blue food coloring The method according to claim 1, in the incubation step, the water temperature in the breeding tank is raised at a rate of 1 to 2 ° C / day, and then maintained at 10 to 15 ° C until shedding,
After confirming the mass molting, the breeding water temperature is raised at a rate of 1 to 2 ° C / day and maintained at 20 to 25 ° C until the confirmation of nesting. delete

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