KR102471402B1 - Jelly-type health functional food manufacturing method for relieving enlarged prostate containing Asian knotweed extract as an active ingredient, and health functional food manufactured thereby - Google Patents

Abstract

Disclosed are a method for preparing a jelly-type health functional food product containing a Reynoutria japonica extract as an active ingredient to alleviate prostate hypertrophy and a health functional food product prepared thereby. The method for preparing a jelly-type health functional food product containing a Reynoutria japonica extract as an active ingredient to alleviate prostate hypertrophy, disclosed herein, comprises: a step a) of obtaining a Reynoutria japonica extract by hot-water extracting Reynoutria japonica; a step b) of obtaining a Reynoutria japonica extract filtrate by filtering the Reynoutria japonica extract; a step c) of obtaining a Reynoutria japonica extract concentrate by concentrating the Reynoutria japonica extract filtrate; a step d) of obtaining a Reynoutria japonica extract mixed solution by mixing the Reynoutria japonica extract concentrate with sub-ingredients including lactose powder and a gelling agent; and a step e) of filling a stick-type package with the Reynoutria japonica extract mixed solution and packaging and cooling the same.

Description

Jelly-type health functional food manufacturing method for relieving enlarged prostate containing Asian knotweed extract as an active ingredient, and health functional food manufactured thereby}

The present invention relates to a method for manufacturing a health functional food containing a Knotweed extract as an active ingredient, and more particularly, to a method for manufacturing a jelly-type health functional food that contains the Knotweed extract as an active ingredient and has an effect of alleviating prostatic hyperplasia, and It relates to a health functional food prepared thereby.

Prostatic hyperplasia is caused by excessive proliferation of smooth muscle cells and epithelial cells in the transitional zone of the prostate, and urination disorders such as frequent urination, nocturia, and delayed urination appear due to bladder obstruction.

The exact cause of this enlargement of the prostate has not been identified, but when testosterone is excessively present in the blood, dihydrotestosterone (DHT) is produced by 5α-reductase (5AR) in the prostate. It is reported that the synthesized DHT is synthesized in large amounts and binds to the androgen receptor (AR) in prostate cells to induce prostate hypertrophy.

In addition, as men age, the secretion of male hormones decreases, and as a result, androgen receptors in prostate cells increase to maintain endocrine balance, it is reported that more DHT binds to cause enlargement of the prostate. .

Treatment methods for prostatic hyperplasia are divided into 5-alpha reductase inhibitors that reduce the size of the prostate gland and alpha blockers that open the urethra of the prostate gland.

5-alpha reductase inhibitors are mainly used as a treatment for prostatic hyperplasia. Finasteride and dutasteride are commercially available, and 5-alpha reductase inhibitors have the activity of dehydrotestosterone (DHT). By inhibiting, it inhibits the action of androgens and acts to reduce the size of the prostate. However, it takes a long time for the effect to appear after taking it, and there are side effects that reduce sexual function.

Alpha-blockers lower the pressure and tension in the prostate and urethra, so Terazosin and Tamsulosin, which are effective quickly, are commercially available, but they lower blood pressure, which can cause dizziness, lethargy, headache, or visual field abnormalities.

Therefore, it is necessary to develop a health functional food that can alleviate, prevent or improve prostatic hyperplasia using natural products capable of minimizing side effects and maximizing functionality.

Looking at the prior literature on health functional food for alleviating and preventing prostatic hyperplasia using these natural products, a health functional food composition for preventing or improving prostatic hyperplasia containing a mixed fermented extract of broiler chicken, milkweed bark, and cucumber apricot (registered patent 10-2022263) , a mixed extract of yacon, saxifrage and calamus, a health food composition containing the same for preventing or improving hair loss or prostatic hyperplasia, and a cosmetic composition for preventing or improving hair loss (Registration Patent 10-1432746), prostatic hyperplasia containing ginseng extract Pharmaceutical compositions and health functional food compositions for prevention or treatment (Patent Publication No. 10-2011-0076347), compositions for treating enlarged prostate containing laurel extract as an active ingredient, and health functional foods (Registration Patent No. 10-2093036) are known. have.

The present invention discloses a health functional food for preventing and improving prostatic hyperplasia through natural products different from the above-mentioned prior art.

Registered Patent No. 10-2022263 Registered Patent No. 10-1432746 Patent Publication No. 10-2011-0076347 Registered Patent No. 10-2093036

The present invention has been devised from the above background, and an object of the present invention is to provide a method for manufacturing a jelly-type health functional food for mitigating prostatic hyperplasia containing a Japanese knotweed extract as an active ingredient and a health functional food prepared thereby.

However, the object of the present invention is not limited thereto, and even if not explicitly mentioned, the purpose or effect that can be grasped from the solution or embodiment of the problem is also included therein, of course.

To achieve the above object, a method for producing a jelly-type health functional food for mitigating prostatic hyperplasia containing the Korean knotweed extract of the present invention as an active ingredient includes the steps of: a) hot water extracting the knotweed root extract to obtain an extract; b) obtaining a filtrate by filtering the knotweed extract; c) obtaining a Hojanggeun extract concentrate by concentrating the Hojanggeun extract filtrate; d) obtaining a Knotweed extract mixture by mixing auxiliary raw materials including lactose powder and a gelling agent with the Knotweed extract concentrate; It is characterized in that it comprises a; e) the step of packing the knotweed extract mixture into a stick cloth, packaging, and cooling.

The step a) may be configured to hot-water extract the knotweed root extract by mixing the knotweed root and water at a weight ratio of 1:5 to 1:8, and heating and pressurizing at 85 ° C to 95 ° C for 10 to 12 hours or more.

The step b) may be configured to obtain the Knotweed extract filtrate by first filtering the Knotweed extract through a 90-110mesh filter net and then filtering it secondarily through a 190-210mesh filter net.

The step c) is to obtain the Knotweed extract concentrate by concentrating the Knotweed extract filtrate using a continuous concentrator at 55 ° C. to 65 ° C. for 10 to 14 hours until the solid content is 55% to 65%. can be configured.

In step c), 21 to 25% by weight of Knotweed extract concentrate, 45.28 to 49.28% by weight of purified water, 13 to 17% by weight of liquid oligosaccharide, 4 to 6% by weight of dextrin, 2 to 4% by weight of lactose powder, and 2 to 3 gelling agents % by weight, anhydrous citric acid 0.8~1.2% by weight, enzyme-treated stevia 0.8~1.2% by weight, Schizandra chinensis concentrate 0.4~0.6% by weight, red ginseng concentrate 0.4~0.6% by weight, red grape concentrate 0.4~0.6% by weight, golden mixed extract 0.2~ 0.4 wt% of zinc gluconate, 0.18 to 0.22 wt% of calcium lactate, 0.18 to 0.22 wt% of calcium lactate, and 0.018 to 0.022 wt% of vitamin D3 were added to the mixing tank, mixed and dissolved, and then dissolved at 75℃ to 85℃ for 25 to 35 minutes. It can be configured to sterilize to obtain a Korean knotweed mixture.

In the step e), the mixed solution of Knotweed Knotweed is filled in a stick and packaged, and after spraying high-temperature steam to sterilize the stick pack filled and packaged in the mixed solution of Knotweed Knotweed, put it in water at room temperature to cool the mixed solution of Knotweed Knotweed in the stick It can be configured to make jellies.

A jelly-type health functional food for mitigating prostatic hyperplasia containing the Kojanggeun extract of the present invention as an active ingredient can be prepared by the above-mentioned manufacturing method.

According to the above, the present invention is a health functional food containing, as an active ingredient, a natural product, Hojanggeun extract, compared to a pharmaceutical treatment for prostatic hyperplasia, which has side effects and has low accessibility, and is effective in alleviating prostatic hyperplasia without side effects.

In particular, the present invention is a liquid oligosaccharide, dextrin, lactose powder, gelling agent (for example, food gel A), anhydrous citric acid, enzyme-treated stevia, Schisandra chinensis concentrate, red ginseng concentrate, red grape concentrate, golden mixed extract, glue As a jelly-type natural health functional food with zinc conate, calcium lactate, and vitamin D3 added as sub-materials, not only is it rich in taste, but it can be consumed by chewing softly, so it can increase the preference for intake and accessibility to see the effect of relieving prostatic hyperplasia. have.

In addition, the various beneficial advantages and effects of the present invention are not limited to the above description, and will be more easily understood in the process of describing specific embodiments of the present invention.

1 is a block diagram showing a method for manufacturing a jelly-type health functional food for mitigating prostatic hyperplasia containing a Japanese knotweed extract as an active ingredient according to an embodiment of the present invention;
Figure 2 is a photograph of a jelly-type health functional food prepared by the manufacturing method of the present invention,
Figure 3 is a view for explaining the experimental procedure of the test for evaluating the efficacy of mitigating prostate hyperplasia of the extract of the Korean knotweed root of the present invention,
Figures 4 to 7 are graphs and micrographs showing the results of the test for evaluating the efficacy of the Korean knotweed extract.

Terms or words used in this specification and claims should not be construed as being limited to their usual or dictionary meanings, and the inventors may appropriately define the concept of terms in order to best explain their invention. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

Therefore, the embodiments described in this specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and do not represent all of the technical ideas of the present invention, so various alternatives can be made at the time of this application. It should be understood that there may be equivalents and variations.

Hereinafter, prior to description with reference to the drawings, matters that are not necessary to reveal the subject matter of the present invention, that is, known configurations that can be added obviously by those skilled in the art, are not shown or specifically described. reveal the sound

The method for manufacturing a jelly-type health functional food for mitigating prostatic hyperplasia containing the Knotweed extract as an active ingredient of the present invention includes the steps of extracting Knotweed Knotweed in hot water to obtain Knotweed Knotweed extract, filtering the Knotweed Knotweed extract and filtrate. Obtaining a step of obtaining a Knotweed extract mixture by concentrating the Knotweed extract filtrate to obtain a Knotweed extract concentrate, mixing the secondary raw materials containing lactose powder and a gelling agent in the Knotweed extract concentrate, obtaining a Knotweed Knotweed extract mixture, It is configured to include; filling the stick into a package and cooling it.

1 is a block diagram showing an embodiment of a method for manufacturing a jelly-type health functional food for mitigating prostatic hyperplasia containing the extract of Janggeun of the present invention as an active ingredient, which will be described in detail with reference to FIG.

First, as a step of preparing the knotweed, the process of checking the appearance of the knotweed, wearing and storing a normal product, washing and removing foreign substances, and selecting materials may be included (s10).

Next, the selected Knotweed and water are mixed in a weight ratio of 1:5 to 1:8 (5 to 8 times the weight of water based on Knotweed), and 10 to 12 at 85 ° C to 95 ° C. By heating and pressurizing for more than an hour, the hot water extract of the Korean knotweed root extract is extracted (s12). At this time, only the Knotweed root extract can be used by taking out the Knotweed root extract obtained by hot water extraction.

Next, the process of filtering the Knotweed extract with a filter net (s14). Specifically, the Knotweed extract is first filtered through a 90-110 mesh filter net and then secondarily filtered through a 190-210 mesh filter net to obtain a Knotweed extract filtrate. get

Next, the Knotweed extract filtrate is concentrated under reduced pressure using a continuous concentrator at 55 ° C to 65 ° C for 10 to 14 hours until the solid content is 55% to 65% to obtain a Knotweed extract concentrate (s14).

Next, 21-25% by weight of Knotweed extract concentrate, 45.28-49.28% by weight of purified water, 13-17% by weight of liquid oligosaccharide, 4-6% by weight of dextrin, 2-4% by weight of lactose powder, 2-3% by weight of gelling agent , anhydrous citric acid 0.8~1.2% by weight, enzyme-treated stevia 0.8~1.2% by weight, Schizandra chinensis concentrate 0.4~0.6% by weight, red ginseng concentrate 0.4~0.6% by weight, red grape concentrate 0.4~0.6% by weight, golden mixed extract 0.2~0.4% by weight %, zinc gluconate 0.18~0.22% by weight, calcium lactate 0.18~0.22% by weight, vitamin D3 0.018~0.022% by weight are put into a mixing tank, mixed and dissolved, and then sterilized at 75℃ ~ 85℃ for 25 to 35 minutes. Obtain a homogenous root mixture (s16).

Next, it is packaged by filling and sealing the packing stick of the knotweed mixture, and sterilizing the stick with the knotweed mixture filled and packed by spraying high-temperature steam at a temperature of 75 ° C to 85 ° C for 30 to 40 minutes, The manufacturing of the product may be completed by immersing the stick cloth in water at room temperature of 5 to 20 ° C. and slowly cooling the knotweed root mixture filled in the stick cloth to make it jellied (s20, s22). At this time, in order to remove water at room temperature on the surface of the stick cloth, the stick cloth may be blown and dried with a blower, and several stick cloth may be packaged and boxed before shipping.

<Experimental Example 1> Experiment to evaluate the efficacy of alleviating prostatic hyperplasia in mice

The following in vivo experiments were performed using mice (male) to confirm the efficacy of the jelly-type health functional food for mitigating benign prostatic hyperplasia containing the extract of Knotweed Knotweed of the present invention as an active ingredient.

1) Preparation of Janggeun Extract

It is prepared by extracting hot water from K. Knotweed using water as a solvent and concentrating to obtain an extract of K. Knotweed at a concentration of 0.1 mg/ml. More specifically, after drying the Knotweed Knotweed, it is pulverized with a grinder to produce a Knotweed Knotweed powder, mixed with 2 mg of Knotweed Knotweed powder in 100ml of water, heated at 90 ° C. for 4 to 5 hours, and then filtered with a 0.45 nm filter After filtering through paper, warming and concentrating to 20 ml through water evaporation to prepare a liquid Knotweed extract of 0.1 mg/ml concentration.

2) Experiment method and result

20 mice (experimental animals) were obtained and each group consisted of 5 mice. A normal group, a group inducing prostatic hyperplasia by testosterone stimulation (control group), a control group administered with testosterone stimulation and finasteride together, and a control group of the present invention In order to confirm the efficacy of the K. Knotweed extract in alleviating prostatic hyperplasia, it was classified into an experimental group administered with testosterone stimulation and K. K. K. K. extract together.

Referring to FIG. 3, the experiment was conducted for 34 days, and the control group (group 2) injected 3 mg of testosterone by intraperitoneal injection based on the weight of the mouse 1 kg once every 2 days for 34 days, and the control group (group 3) 3 mg of testosterone based on the weight of 1 kg of mouse and 5 mg of finasteride based on the weight of 1 kg of mouse were injected intraperitoneally once every 2 days for 34 days. The experimental group (group 4) injected testosterone once every 2 days for 34 days. Based on the weight of 1 kg of mouse, 3 mg was injected intraperitoneally, and the prepared Hojanggeun extract was fed to 50 mg based on the weight of 1 kg of mouse.

During this 34-day experiment, the weight of each group was measured at 3-day intervals, and the results are shown in FIG. 4 . As in the normal group, control group, and control group (groups 1, 2, and 3), the experimental group (group 4) showed no decrease in body weight during the experiment period and showed the same increase in weight, proving that there is no toxicity caused by the extract of K. did

In addition, after the 34-day experimental process, the mouse was euthanized, and the object weight was measured immediately in the euthanized state, and the prostate was removed by laparotomy to measure the prostate weight to measure the ratio of prostate weight per object weight, The results are shown in FIG. 5 .

Referring to FIG. 5, based on the prostate weight ratio per object weight for the normal group (group 1) as 1, the prostate weight ratio per object weight in the control group, control group, and experimental group is compared to the normalized group and displayed. (group 3) demonstrated that finasteride inhibited testosterone-induced prostate enlargement, and similarly, in the experimental group (group 4), it was demonstrated that the K. K. root extract suppressed testosterone-induced prostate enlargement.

In addition, the level of prostate-specific antigen (PSA) in the prostate for each experimental group was measured, and the results are shown in FIG. 6 .

PSA, or prostate specific antigen, is a proteolytic enzyme synthesized in prostate epithelial cells, and is rarely expressed in tissues other than the prostate. It is a useful tumor marker used for screening prostate cancer and increases in prostatic hyperplasia. As shown in FIG. 6, it was demonstrated that the PSA level of the control group (group 3 and the experimental group (group 4) was at an equal level, and that the Korean knotweed extract exhibited the alleviating effect of benign prostatic hyperplasia.

In addition, H&E staining results were observed by H&E staining of mouse prostates for each experimental group and photographed under a microscope, and also, COX2 marker and iNOS marker, which are inflammatory markers, were observed by photographing under a microscope through specific antibody staining, and the results are shown in FIG. 7 shown in

Looking at the H&E staining results, compared to the control group (group 2), the control group (group 3) and the experimental group (group 4) showed a distribution of large circular rings. As compared to group 2), the control group (group 3) and the experimental group (group 4) had lower staining levels, which proved that finasteride and knotweed extract inhibited the inflammatory markers COX2 and iNOS.

Through the above results, it can be seen that the Knotweed extract is effective in alleviating benign prostatic hyperplasia, and the jelly-type health functional food of the present invention containing the Knotweed Knotweed extract as an active ingredient is also effective in alleviating benign prostatic hyperplasia.

In the above, the present invention has been shown and described in relation to preferred embodiments for illustrating the principles of the present invention, but the present invention is not limited to the configuration and operation as described. Rather, those skilled in the art will appreciate that many changes and modifications to the present invention can be made without departing from the spirit and scope of the appended claims. Accordingly, all such appropriate changes and modifications and equivalents should be regarded as belonging to the scope of the present invention.

Claims (7)

a) hot-water extracting Knotweed extract by mixing Knotweed and water at a weight ratio of 1:5 to 1:8 and heating and pressurizing at 85 ° C to 95 ° C for 10 to 12 hours;
b) firstly filtering the Knotweed extract obtained through step a) through a 90-110mesh filter net and then secondarily filtered through a 190-210mesh filter net to obtain a Knotweed extract filtrate;
c) obtaining a knotweed extract concentrate by concentrating the filtrate of the knotweed extract using a continuous concentrator at 55 ° C to 65 ° C for 10 to 14 hours until the solid content is 55% to 65%;
d) 21-25% by weight of the Knotweed extract concentrate, 45.28-49.28% by weight of purified water, 13-17% by weight of liquid oligosaccharide, 4-6% by weight of dextrin, 2-4% by weight of lactose powder, 2-3% by weight of gelling agent , anhydrous citric acid 0.8~1.2% by weight, enzyme-treated stevia 0.8~1.2% by weight, Schizandra chinensis concentrate 0.4~0.6% by weight, red ginseng concentrate 0.4~0.6% by weight, red grape concentrate 0.4~0.6% by weight, golden mixed extract 0.2~0.4% by weight %, zinc gluconate 0.18 ~ 0.22% by weight, calcium lactate 0.18 ~ 0.22% by weight, vitamin D3 0.018 ~ 0.022% by weight to obtain a homogenous root extract mixture;
e) Filling and packaging the knotweed extract mixture in a stick cloth, spraying high-temperature steam on the packed stick cloth, and then immersing the stick cloth in room temperature water at 5 to 20 ° C. A method for producing a jelly-type health functional food for mitigating prostatic hyperplasia containing the Knotweed extract as an active ingredient, characterized in that it comprises a; packing and cooling step of cooling the Knotweed extract mixture in the gel to make it jellied.
delete delete delete delete delete A jelly-type health functional food for relieving benign prostatic hyperplasia containing, as an active ingredient, the extract of the Korean knotweed root prepared by the manufacturing method according to claim 1.

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