KR102457989B1 - Process for cultivating plant with organic germanium-ion water - Google Patents

Abstract

The present invention relates to a method of cultivating a plant containing a germanium component using organic germanium ion water, and more particularly, to synthesize insoluble metal germanium into ionic organic germanium to provide a good state of absorption for plants. , combine the oxygen group of germanium oxide with a chlorine group to synthesize germanium tetrachloride, and convert the acid (RCOOH) group (or hydroxyl group (ROH)) of the amino acid to an anion to synthesize it into germanium ions that are soluble in water. By making it a counterpart (RCOO ^- , Or RO ^- ) Cationic germanium (Ge ⁴⁺ ) and ion state that is well soluble in water, and the synthesized organic germanium ion is applied to the plant to reduce the harmfulness. is about According to the present invention, functional plants having safety and usefulness can be provided by changing insoluble metal germanium to organic germanium in an ionic state and providing it in a state with good absorption for plants.

Description

Cultivation method of plants containing germanium components using organic germanium ion water {Process for cultivating plant with organic germanium-ion water}

The present invention relates to a method of cultivating a plant containing a germanium component using organic germanium ion water, and more particularly, to synthesize insoluble metal germanium into ionic organic germanium to provide a good state of absorption for plants. , combine the oxygen group of germanium oxide with a chlorine group to synthesize germanium tetrachloride, and convert the acid (RCOOH) group (or hydroxyl group (ROH)) of the amino acid to an anion to synthesize it into germanium ions that are soluble in water. by making it a counterpart (RCOO ^- , Or RO ^- ) Cationic germanium (Ge ⁴⁺ ) and water-soluble ions are synthesized in the state, and the synthesized organic germanium ions are applied to plants to reduce the harmfulness. A method of growing plants containing germanium components using organic germanium ion water. is about

Germanium is a carbon group element belonging to Group 4B of the periodic table, and organic germanium with high purity holds and accumulates cancer cells, toxins, pollutants, and heavy metals in the human body within about 20-30 hours after completing various tasks in the human body. It is completely excreted from the human body through urine, so it is known to be very safe because it does not leave any toxicity [Asai Germanium Research Institute, Tokyo, Japan].

Also, it has been reported that organic germanium acts as an interferon derivative in the human body, suppresses tumors and malignant tumors, lowers blood pressure in animals, or has an analgesic effect.

On the other hand, due to the usefulness of germanium as described above, a method of growing a crop for increasing the germanium content in a cultivated crop so that germanium can be safely ingested is being researched and developed.

In order to cultivate a crop containing a large amount of organic germanium, there are largely two types of cultivation methods known so far.

One is to find soil or stones with high germanium content, crush them, sprinkle them on the soil like fertilizer, and then grow crops. The other is to develop and fertilize fertilizers with an artificially increased germanium content.

In the former case, minerals with a high germanium content are extremely rare and the content is also not uniform, so the expected effect is very insignificant. is still desired.

Korea Publication No. 10-2001-0000513 (Jan. 05, 2001) Korea Registration No. 10-0513267 (August 31, 2005) Korea Registration No. 10-1517074 (April 27, 2015)

As a result of intensive research in order to solve the problems according to the prior art as described above and provide a product with secured functionality, the present inventors synthesize insoluble metal germanium into organic germanium in an ionic state and provide water-soluble plants as described below. It has been found that a useful and safe product can be provided due to low toxicity when applied to plants and improved absorption to plants, and completed the present invention.

Accordingly, an object of the present invention, in one aspect, is to synthesize insoluble metal germanium into organic germanium in an ionic state to provide a method of growing a plant containing a germanium component using organic germanium ion water with good absorption to plants and low toxicity is in doing

The above object of the present invention is

a) heating germanium oxide with hydrochloric acid to obtain germanium tetrachloride;

b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;

c) diluting the organic germanium ions with purified water to obtain an organic germanium aqueous solution; and

d) injecting the organic germanium aqueous solution into a plant to obtain a plant containing organic germanium; can be achieved by a method of growing a plant containing organic germanium, comprising:

According to the present invention, functional plants having safety and usefulness can be provided by changing insoluble metal germanium to organic germanium in an ionic state and providing it in a state with good absorption for plants.

1 is a process diagram for a method of growing a plant containing organic germanium of the present invention.
2 is a view for explaining the synthesis process of organic germanium ions according to the present invention.
3 is a test result of measuring the content of germanium synthesized using ascorbic acid.
4 is a test result of measuring the content of germanium synthesized using low molecular weight collagen.
5 is a test result of measuring the germanium content of sprouted barley grown hydroponically.
6 is a test result of measuring the germanium content of sprouted barley powder grown hydroponically.

The present invention, in one aspect,

a) heating germanium oxide with hydrochloric acid to obtain germanium tetrachloride;

b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;

c) diluting the organic germanium ions with purified water to obtain an organic germanium aqueous solution; and

d) injecting the organic germanium aqueous solution into a plant to obtain a plant containing organic germanium; provides a method for cultivating a plant containing organic germanium, comprising: a.

The present invention, in a further aspect,

The organic compound provides a method of cultivating a plant containing organic germanium, characterized in that ascorbic acid, cysteine, salicylic acid, sialyllactose or amino acids.

The present invention, in another further aspect,

Step a) is carried out by heating and reacting with hydrochloric acid,

In step b), germanium tetrachloride is reacted with an organic compound having a hydroxyl group or a carboxyl group, acetone and baking soda under reflux, and then the acetone is primarily removed using a rotary concentrator, and the acetone is secondarily removed by pumping it with a vacuum pump. It provides a method of growing a plant containing organic germanium, characterized in that.

The present invention, in another further aspect,

The plant provides a method of cultivating a plant containing organic germanium, characterized in that it is seafood including grains including sprouted barley, fruits or vegetables including apples, pears or strawberries, seaweed, seaweed, or kelp. .

The present invention, in another further aspect,

It provides a method of cultivating a plant containing organic germanium, comprising: supplying an organic germanium aqueous solution to sprouted barley in step d) to obtain a plant containing organic germanium by hydroponic cultivation.

Hereinafter, a method for cultivating a plant containing organic germanium according to the present invention will be described in more detail with reference to the accompanying drawings.

The terms or words used in the present specification and claims should not be construed as being limited to their ordinary or dictionary meanings, and the inventor may properly define the concept of the term in order to best describe his invention. Based on the principle that there is, it should be interpreted as meaning and concept consistent with the technical idea of the present invention. Accordingly, the embodiments described in the present specification and the configurations described in the drawings are only the most preferred embodiment, and do not represent all of the technical spirit of the present invention, and thus various equivalents and It should be understood that there may be variations.

The present invention synthesizes germanium tetrachloride by combining the oxygen group of germanium oxide, which is easy to contact, with a chlorine group, in order to provide a state with good absorption for plants by synthesizing insoluble metal germanium into organic germanium in an ionic state, and To synthesize the germanium ion in a soluble state, the acid (RCOOH) group (or hydroxyl group (ROH)) of the amino acid is made into an anionic counterpart (RCOO ^- , Or RO ^- ) Cationic germanium (Ge ⁴⁺ ) and water-soluble ions are synthesized in an ionic state, and the synthesized organic germanium ions are applied to plants to lower the harmfulness.

As shown in the figure, the cultivation method for plants containing organic germanium of the present invention largely includes a germanium tetrachloride production step, an organic germanium ion production step, an aqueous solution production step, and a plant application step. Hereinafter, each individual step will be described in detail.

a) Germanium tetrachloride manufacturing step

In order to make metal germanium easier to dissolve in water, a process of synthesizing germanium oxide into germanium chloride is required. Germanium tetrachloride is obtained by heating germanium oxide with hydrochloric acid as shown in Formula 1 below. For this purpose, it may be preferable to synthesize germanium tetrachloride by combining a chlorine group instead of oxygen with germanium oxide, which is easy to purchase. The heating time is suitable for 20 to 40 minutes, and the temperature suitable for the reaction is 90 to 120 °C. Subsequently, the reactant is distilled at 80 to 90° C. using a distillation apparatus to obtain germanium tetrachloride.

b) Preparation of germanium ions using germanium tetrachloride

b1) Preparation of germanium ions bound to a hydroxyl group

In order to synthesize organic germanium ions that are easily soluble in water, it is necessary to convert germanium chloride into an ionic state using amino acids or the like.

In this embodiment, the anion of the hydroxyl group (-OH) of ascorbic acid binds to a germanium cation to form a germanium ion.

As shown in Formula 2 below, the mixture is stirred with an organic compound having a hydroxyl group such as acetone, baking soda and ascorbic acid at room temperature for 5 to 20 minutes. Then, germanium tetrachloride is added to a reaction vessel and reacted under reflux for 6 to 10 hours to obtain ionic organic germanium. In this reaction, hydrochloric acid is produced as a by-product, and the hydrochloric acid is removed by neutralizing with baking soda (NaHCO ₃ ).

After the reaction is completed, the acetone is carefully removed using a rotary evaporator because there is a solid that may be doped. After that, it is pumped to a vacuum pump for 4 hours to remove a very small amount of acetone.

b2) Preparation of germanium ions bound to carboxyl groups

In order to synthesize organic germanium ions that are easily soluble in water, it is necessary to convert germanium chloride into an ionic state using amino acids.

In the present embodiment, it is a route in which the anion of the carboxyl group of the low molecular weight collagen bonds with the germanium cation to form the germanium ion.

As shown in Chemical Formula 3, acetone, baking soda and low molecular weight collagen are added and stirred at room temperature for 5 to 20 minutes. Then, germanium tetrachloride is added to a reaction vessel and reacted under reflux for 6 to 10 hours to obtain ionic organic germanium. In this reaction, hydrochloric acid is produced as a by-product, and the hydrochloric acid is removed by neutralizing with baking soda (NaHCO ₃ ).

After the reaction is completed, the acetone is carefully removed using a rotary evaporator because there is a solid that may be doped. After that, it is pumped to a vacuum pump for 4 hours to remove a very small amount of acetone.

The organic compound is not limited thereto, and may include ascorbic acid, cysteine, salicylic acid, and sialyllactose.

c) Preparation of organic germanium aqueous solution

Organic germanium ions are obtained in the form of an aqueous solution by using distilled water or the like. An appropriate concentration of organic germanium ions in the aqueous solution may be 10 to 10000 ppm.

d) injection into the plant

The above organic germanium can be injected into various plants in various ways.

For example, organic germanium may be supplied by adding it in the form of fertilizer or liquid fertilizer, or through a cultivation method such as hydroponics in which an aqueous solution is brought into contact with the roots of plants.

As an example, in the case of sprouted barley, the outer barley seeds are soaked in the organic germanium ionized water (100~10,000ppm) for 8~24 hours, then the ionized water is removed, put in a seedling box, and covered with plastic until germination occurs. After germination a day later, keep the room temperature at 18 ~ 20℃ in the seedling box and give the germanium solution with a sprayer about 2-3 times a day so that the moisture does not dry out. After 7 to 10 days after sowing, the seeds will grow to about 15 to 20 cm, and you can harvest them at this time.

In the same manner, organic germanium ionized water according to the present invention is not limited thereto, but is applied to cereals, fruits or vegetables including apples, pears or strawberries, seaweed, seaweed, or seafood including seaweed to increase the content of organic germanium. can do it

<Example>

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention in more detail, and it is obvious to those skilled in the art that the scope of the present invention is not limited by these examples according to the gist of the present invention.

Example 1: Preparation of germanium tetrachloride

After installing a condenser in a 500 mL 3-neck flask, the cooling water of the condenser was maintained at -40°C. 2 g of germanium oxide (GeO ₂ ) was added to 300 mL of 6N hydrochloric acid and heated for 30 minutes. After removing the condenser and installing a distillation apparatus, it was distilled at 86.5° C. to obtain 3 g of germanium tetrachloride (GeCl ₄ ).

Example 2: Preparation of germanium ions using germanium tetrachloride

500 mL of dried acetone, 4.1 g of ascorbic acid, and 2 g of fine powdered baking soda (NaHCO ₃ ) were added to a 1000 mL dry round-bottom flask and stirred at room temperature for 10 minutes. Germanium tetrachloride (GeCl ₄ ) 1 g (0.53 mL) was added to the reaction vessel and refluxed for 8 hours. After completion of the reaction, acetone was carefully removed using a rotary evaporator, and then acetone was removed in a vacuum pump for 4 hours to remove a very small amount of acetone to obtain 2.5 g of organic germanium ions. 500 mL of distilled water was added to the flask from which the acetone was removed to obtain organic germanium ionized water.

Example 3: Preparation of germanium ions bound to carboxylic acids

500 mL of dried acetone, 6.6 g of low molecular weight collagen (Gly-Pro-Hyp), and 2 g of fine powdered baking soda (NaHCO ₃ ) were added to a 1000 mL dried round flask and stirred at room temperature for 10 minutes. Germanium tetrachloride (GeCl ₄ ) 1 g (0.53 mL) was added to the reaction vessel and refluxed for 8 hours.

After the reaction was completed, the acetone was carefully removed using a rotary concentrator, and then the acetone was removed in a vacuum pump for 4 hours to remove a very small amount of acetone to obtain 2.5 g of organic germanium ions. 500 mL of distilled water was added to the flask from which the acetone was removed to obtain organic germanium ionized water.

Example 4: Hydroponic cultivation of sprout barley

First, soak 180~200g of barley seeds in germanium ionized water (200ppm) for 12 hours, drain the ionized water, put it in a seedling box (60cm x 30cm), and cover it with plastic until it sprouts. After germination a day later, in the seedling box, the room temperature was maintained at 18 ~ 20 ℃, and the germanium solution was supplied with a sprayer about 2-3 times a day to the extent that the moisture did not dry out. After 7 to 10 days after sowing the seeds, they grow to about 15 to 20 cm. The seeds were harvested and used in subsequent test examples.

Test Example 1: Germanium component analysis

The components obtained in Example 2 were subjected to ICP (Inductively Coupled Plasma) emission spectroscopy to analyze the components. 3 is a test result of measuring the content of germanium synthesized using ascorbic acid, and the content of ionic germanium was found to be 1042.8 mg/kg.

Test Example 2: Germanium component analysis

The components (germanium ions prepared using carboxylic acid) obtained in Example 3 were requested for ICP (Inductively Coupled Plasma) emission spectroscopy to analyze the germanium components. 4 is a test result of measuring the content of germanium synthesized using low molecular weight collagen. As a result of the test, the content of ionic germanium was found to be 1440.6 mg/kg.

Test Example 3: Analysis of sprouted barley components

The sprouted barley harvested in Example 4 and the dried powdered sprouted barley using a dryer were requested for ICP (Inductively Coupled Plasma) emission spectroscopy and component analysis. 5 is a test result of measuring the germanium content of sprouted barley grown hydroponically, and FIG. 6 is a test result of measuring the germanium content of dry sprouted barley powder grown hydroponically.

In the case of the harvested sprouted barley, the germanium content was found to be 350.1 mg/kg, whereas the dry powder was found to be 799.6 mg/kg, indicating that the drying process was more useful.

Test Example 4: Acute toxicity test

A toxicity test was performed on the organic germanium ionized water according to Example 2, and the presence or absence of toxicity was observed. For laboratory animals, 60 SD rats, male and female, weighing 105±4g and 95±3g, aged 4 and 5 weeks, were used and tested using the extract of the present invention and distilled water as a negative control. First, the rats were subjected to about 1 in a laboratory breeding box equipped with a temperature of 22±2° C., a relative humidity of 53±2%, a light-dark cycle of fluorescent lighting (09:00 on-18:00 off), and an illuminance condition of 150-300 Lux. After acclimatization over a period of about a week, only healthy animals were selected and divided into groups so that the average weight was matched, and administered orally at a dose of 20ml/kg once a day for 14 days. , motility, appearance, autonomic nerves, weight change, and the presence or absence of dead animals were checked.

According to the experimental results, there was a change within 5% of body weight during the experiment period, but there was no significance. Since it was impossible to calculate the lethal dose, the LD ₅₀ was found to be 20ml/kg BW or more, and no specific general symptoms or specific lesions were observed at autopsy.

As described above, the direction of use of organic germanium has been suggested according to the present invention, and this provides an opportunity to additionally generate interest and roles from people involved in the manufacturing industry, thereby providing an opportunity to take the lead in job creation and well-being food culture. is judged In addition, since social and cultural health-related issues continue to emerge in the present and in the future in the mass media, etc., many palatable and functional foods such as the present invention have been developed, suggesting a direction to solve various environmental and social problems.

As described above, although the present invention has been described with reference to the limited embodiments and drawings, the present invention is not limited to the above embodiments, which are various modifications and Transformation is possible. Accordingly, the spirit of the present invention should be understood only by the claims set forth below, and all equivalents or equivalent modifications thereof will fall within the scope of the spirit of the present invention.

Claims (5)

a) heating germanium oxide with hydrochloric acid to obtain germanium tetrachloride;
b) reacting germanium tetrachloride with an organic compound having a hydroxy group or a carboxy group to obtain organic germanium in an ionic form;
c) diluting the organic germanium ions with purified water to dissolve the organic germanium ions so that the final concentration of organic germanium is 10 to 10000 ppm to obtain an aqueous solution; and
d) injecting the organic germanium aqueous solution into the plant to obtain a plant containing organic germanium;
Step a) is carried out by heating and reacting with hydrochloric acid,
In step b), germanium tetrachloride is reacted with an organic compound having a hydroxyl group or a carboxyl group, acetone and baking soda under reflux, and then the acetone is first removed using a rotary concentrator, and the acetone is secondarily removed by pumping it with a vacuum pump. perform,
The organic compound is a method of growing a plant containing organic germanium, characterized in that ascorbic acid, cysteine, salicylic acid, or sialyllactose. delete delete The method of claim 1,
The plant is a method of cultivating a plant containing organic germanium, characterized in that it is cereals including sprouted barley, fruits or vegetables including apples, pears or strawberries, seaweed, seaweed, or seafood including kelp. The method according to claim 1,
Method of cultivating a plant containing organic germanium comprising the; step d) in step d) by supplying an aqueous solution of organic germanium to sprouted barley and hydroponically culturing to obtain a plant containing organic germanium.

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