KR102410704B1 - Composition for preventing, improving or treating bone disease comprising Euonymus sp. plant extract as effective component - Google Patents

Abstract

The present invention relates to a composition for the prevention, improvement or treatment of bone diseases, comprising a plant extract of the genus Acariosa as an active ingredient. Therefore, the plant extract of the genus Sagittarius of the present invention can be usefully used as a health functional food or pharmaceutical for the prevention, improvement or treatment of bone diseases.

Description

A composition for preventing, improving or treating bone disease containing a plant extract of the genus Sagittarius as an active ingredient, comprising: Euonymus sp. plant extract as effective component}

The present invention relates to a composition for preventing, improving, or treating bone diseases, comprising an extract of a plant of the genus Saraceae as an active ingredient.

Bone (bone) supports the body's soft tissues and body weight and surrounds internal organs to protect internal organs from external impact. In addition, it is one of the important parts of the human body that not only structurally supports muscles or organs, but also stores substances such as calcium and other essential minerals in the body, such as phosphorus and magnesium.

Bone is a very complex tissue composed of cells, collagenous matrix and mineral components. They provide basic and diverse functions, such as providing a microenvironment necessary for hematopoiesis, as well as protecting organs. In addition, it is one of the important parts of the human body that not only structurally supports muscles or organs, but also stores substances such as calcium and other essential minerals in the body, such as phosphorus and magnesium.

Adult bones that have completed growth do not stop, and the old bone is removed and replaced with new bone until the day of death. This is called bone remodeling.

The turnover of removing old bones and replacing them with new ones is essential to restore microscopic damage to bones caused by growth and stress and to maintain proper bone function. It is known that two types of cells are largely involved in bone remodeling. One of the two cells is an osteoblast that creates bone, and the other is an osteoclast that destroys the bone. Osteoblasts produce RANKL (receptor activator of nuclear factor-κB ligand) and its inducible receptor (decoy receptor) OPG (osteoprotegerin). When RANKL binds to receptor activator of nuclear factor-κB (RANK), a receptor on the surface of osteoclast progenitor cells, the osteoclast progenitor cells mature into giant multinucleated osteoclasts (PLturation) and bone resorption occurs. . However, when OPG binds to RANKL, the binding between RANKL and RANK is blocked, inhibiting the formation of osteoclasts and preventing excessive bone resorption. The activity of these osteoclasts leads to resorption or destruction of old bone, which makes a hole in the bone and releases a small amount of calcium into the bloodstream, which is used to maintain body functions.

On the other hand, Euonymus alata ( Euonymus alata ) has a size and a width of 3 m, and the stem and branches are divided a lot. It resembles an arrowhead tree, but has no wings on the stem. The leaves are opposite, oval, with fine saw teeth on the edge and short petioles. In May, three yellow-green flowers are gathered in the leaf axil and run in a striae. There are 4 petals, sepals, and stamens each. The capsule fruit ripens in red in October, and the white seeds are wrapped in a yellowish red hull. It is a deciduous broad-leaved shrub that grows in rocky areas at the foot of mountains and mid-mountain all over the country.

The arrowhead tree ( Euonymus alatus ) has two nodes opposite each other, and the leaf shape is egg-shaped and both ends are pointed. The length of the leaf is 3~5cm, the edge has small saw teeth, and the back side of the leaf is grayish green. Light green flowers bloom in May, and round and flat fruits ripen to brown in autumn. Similar to the arrow tree, there are spruce, ash, and ash trees.

Fertile soil with deep soil depth and water holding capacity is good, and it grows well in the sun with medium water, tolerates the cold well, overwinters in the open field, and can be cultivated nationwide. The shoots are edible as a vegetable, and are known to be effective in the treatment of cancer such as stomach cancer or esophageal cancer, and are known to be effective in curing diabetes and stinging of scissors. In addition, the fruit was decoctioned for a long time and used to treat skin diseases, and it is effective for high blood pressure, arteriosclerosis, cough phlegm, menstrual irregularity, menstruation, and abdominal pain due to eohyeol after childbirth.

Euonymus sieboldiana ) lives mainly in the foothills of high mountains at a height of 500 to 1300 m, along rivers and on the seashore. Leaves about 5 to 15 cm long hang opposite each other. It is a long oval with a sharp tip, and has irregular and dull fine serrations on the edge. It is hairless and is dyed bright red in autumn. It was used for cough, blood circulation disorders, menstrual pain, back pain, myalgia, arthralgia, and dermatitis.

The willow tree ( Euonymus trapococcus ) grows in mountainous areas. The leaves are opposite, long oval or egg-shaped oval, without hairs, and both ends are pointed. It is about 8cm long and has fine saw teeth on the edge. Flowers bloom in June in yellow mixed with green and hang in axillary inflorescences. There are 4 sepals, petals, and stamens. The fruit is a capsule, round, ripens in reddish brown from September to October, and has 4 wings. Wings are 12mm long and 4mm wide, and small fruit stalks are 7-10mm long. The young leaves are edible and used as firewood, and are distributed north of Jeollabuk-do and Gyeongsangbuk-do in Korea.

Euonymus bungeanus grows at the foot of the mountain. There is also a small arbor with a height of about 5 m. Small branches are round in shape. The leaves are opposite, broad oval or elliptical lanceolate, 5-10 cm long. Both ends are pointed and the edges have blunt teeth. Flowers bloom in May-June with greenish white, and hang in axillary inflorescences. The peduncle is 1~2cm, and 3~7 flowers hang. The calyx is divided into 4 parts, and there are 4 petals and 4 stamens. The fruit is a capsule with a diameter of about 1cm, ripens in October to a light red color, is deeply divided into 4 parts, and has no wings. The agaricous skin is yellow in color after fertilization. Plant it as a garden tree. It is distributed in Korea (Jeju Island, Gyeongsangnam-do, Jeollanam-do), China, and Mongolia.

The evergreen tree ( Euonymus fortunei ) has a creeping stem with a length of 10 m or more, and air roots come out and stick to other objects. Opposite leaves are oval or ovate, thick in quality, and have dull serrated edges. The surface is dark green, the back side is gray-green, and there are no hairs on both sides. From May to June, about 15 flowers hang in the form of bristle from the leaf axil. There are 4 petals, sepals, and stamens each, and there is 1 pistil. The fruit is a capsule, ripens in red in October, and splits into 4 parts to release the seeds.

Euonymus japonicus is a short evergreen tree that grows about 2-3m in height at the foot of the seashore south of Gangwon-do and Gyeonggi-do. Nowadays, it is often cultivated as a garden tree or planted on a fence to enjoy the greenery of all seasons. It is resistant to sea winds and bases, grows well in wetlands and dry areas, and the leaves are opposite to the stem and have a long oval shape with a length of 3 to 7 cm, and are thick and dark green with a glossy surface. In oriental medicine, fern bark is used as a diuretic or tonic, and is also used for arthritis, neuralgia, back pain, dentition, pain, and blood circulation. Twigs and roots are also used as anti-inflammatory agents. In folklore, it is said that the root of the perennial tree is decoctioned and taken as medicine, and it is said to be effective in menstrual irregularities, a female disease.

Confession tree ( Euonymus oxyphyllus ) has 5 petals and is light purple, and 3~8cm long leaves hang on the branches opposite to each other. It is egg-shaped with a sharp tip, has fine serrated edges, has little hair, and is dyed red in autumn. It blooms in light purple at the place with leaves in May-June. A flower stalk that splits opposite each other comes out, and a flower hangs at each node and end, and only one flower runs. Male and female flowers bloom on different trees. There is 1 pistil, 5 stamens, 5 petals, and 5 sepals. From September to October, round and flat fruits with a diameter of about 1cm in 5 pieces ripen in red or purple. When ripe, the skin of the fruit splits into 5 parts to reveal red seeds. It is used for allodynia, poor circulation, fracture pain and joint pain.

Boxwood ( Euonymus pauciflorus ) is harvested in spring and dried in the sun for use. Diabetes mellitus, arteriosclerosis, irregular menstruation, postpartum pain after childbirth, 10g dried in 700㎖ water and drink as a decoction. Apply dried decoction to the boil.

Euonymus sachalinensis ( Euonymus sachalinensis ) grows upright to about 4 m, has open branches, and young branches are brown. The leaves are opposite, oval or obovate, with fine saw teeth on the edge, and no hairs on both sides. In June-July, purple flowers hang from the leaf axils as bristle. Petals and stamens are 5 each. The fruit is round, has 5 wings, and ripens red in September. Inside are dark pink seeds.

As a prior art related to plants of the genus Saturn, Korean Patent Application Laid-Open No. 2004-0080641 discloses a composition for preventing and treating diabetes containing an arrowroot extract, and Korean Patent Publication No. 2020-0027455 discloses a respiratory disease using the arrowroot extract. Although a composition for improvement has been disclosed, there is no disclosure of a composition for preventing, improving, or treating bone disease containing the extract of the genus oleracea of the present invention as an active ingredient.

The present invention has been derived from the above needs, and the present invention provides a composition for preventing, improving, or treating bone diseases, comprising an extract of a plant of the genus Sagittarius as an active ingredient, wherein the extract of the plant of the genus Sagittarius contains osteoclasts. By confirming that there is an effect of inhibiting differentiation and promoting differentiation of osteoblasts, and maintaining or increasing the survival rate of osteoclast progenitors, the present invention has been completed.

In order to achieve the above object, the present invention is the bark of a birch tree; leaves of willow ash; a mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; It provides a health functional food composition for the prevention or improvement of any one bone disease selected from osteoporosis, osteoporosis, osteomalacia, and bone formation disorders, containing an extract of any one of the arrowroot plants selected from among them as an active ingredient.

In addition, the present invention is the bark of a birch tree; leaves of willow ash; a mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; It provides a pharmaceutical composition for the prevention or treatment of any one bone disease selected from osteoporosis, osteoporosis, osteomalacia, and osteogenic disorders, which contains an extract of any one of the genus Arboraceae selected from among the extracts as an active ingredient.

The present invention relates to a composition for the prevention, improvement or treatment of bone diseases, comprising a plant extract of the genus Arachnius as an active ingredient, wherein the plant extract of the genus Saturn has the effect of inhibiting osteoclast differentiation and promoting osteoblast differentiation, and progenitor of osteoclasts. It has the effect of maintaining or increasing the viability of cells.

1 shows osteoclast formation inhibition according to the treatment concentration of extracts for each part of the genus Sagittarius plant (H. It is the result of confirming the effect. * and ** indicate that the number of osteoclasts in the experimental group treated with the Achilles plant extract of the present invention was statistically significantly decreased compared to the control group that was not treated with the Achilles plant extract of the present invention, * is p<0.05, ** is p <0.01.
2 is an osteoclast progenitor cell survival rate according to the treatment of extracts for each part of the genus Sagittarius plant (H. is the result of checking * and ** indicate that the cell viability of the experimental group treated with the genus genus plant extract was statistically significantly increased compared to the control group that was not treated with anything, * indicates p<0.05, and ** indicates p<0.01.
Figure 3 shows the osteoblast differentiation promoting effect according to the treatment of extracts for each part of the plant of the genus Sagittary (H. is the result of checking * and ** indicate that there was a statistically significant difference in osteoblast differentiation of the group treated with the genus oleracea plant extract compared to the control group that was not treated with anything, * indicates p<0.05, and ** indicates p<0.01.

The present invention is the bark of a birch tree; leaves of willow ash; A mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; It relates to a health functional food composition for the prevention or improvement of any one bone disease selected from osteoporosis, osteoporosis, osteomalacia, and bone dysplasia, containing a plant extract of any one selected from the genus Euonymus sp .

The plant extract of the genus Sagittary may be prepared by a method comprising the following steps, but is not limited thereto:

(1) extracting by adding an extracting solvent to the plants of the genus oleracea;

(2) filtering the extract of step (1); and

(3) Concentrating the filtered extract of step (2) under reduced pressure and drying to prepare an extract.

In step (1), the extraction solvent is preferably selected from water, a C ₁ to C ₄ lower alcohol or a mixture thereof, and more preferably methanol, but is not limited thereto.

In the above preparation method, the extraction method may use all conventional methods known in the art, such as filtration, hot water extraction, immersion extraction, reflux cooling extraction, and ultrasonic extraction. The extraction solvent is preferably added by adding 1 to 20 times the weight of the dried arrowroot plant, more preferably 5 to 15 times. The extraction temperature is preferably 20 ~ 110 ℃, but is not limited thereto. In addition, the extraction time is preferably 0.5 to 10 hours, more preferably 0.5 to 5 hours, but is not limited thereto. In the above method, the vacuum concentration in step (3) is preferably, but not limited to, a vacuum vacuum concentrator or a vacuum rotary evaporator. In addition, drying under reduced pressure, vacuum drying, boiling drying, spray drying or freeze drying is preferable, but is not limited thereto.

It is preferable that the extract of the genus Sagittarius plant is obtained by extracting one or more parts selected from leaves, flowers, branches, stems, bark (stem bark), fruits, underground parts, and outposts of the asteraceae plant, but is not limited thereto. .

The plant extract of the genus Arboraceae inhibits the differentiation of osteoclasts and promotes bone formation, and the bone disease may be caused by bone loss, but is not limited thereto.

The composition is preferably prepared in any one formulation selected from powder, granule, pill, tablet, capsule, candy, syrup and beverage, but is not limited thereto.

When the health functional food composition of the present invention is used as a food additive, the extract of the genus oleracea may be added as it is, or it may be used together with other foods or food ingredients, and may be suitably used according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of its use (prevention, health or therapeutic treatment). In general, in the production of food or beverage, the composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health and hygiene or health control, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount greater than the above range.

There is no particular limitation on the type of the food. Examples of foods to which the extract can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, There are alcoholic beverages and vitamin complexes, and includes all health functional foods in the ordinary sense.

When the composition of the present invention is used as a health drink, it may contain various flavoring agents or natural carbohydrates as an additional component like a conventional drink. The above-mentioned natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, polysaccharides such as dextrin and cyclotenstrin, and sugar alcohols such as xylitol, sorbitol and erythritol. As the sweetener, natural sweeteners such as taumarin and stevia extract, synthetic sweeteners such as saccharin or aspartame, and the like can be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 g of the composition of the present invention. The composition of the present invention contains various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal agents, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may contain a carbonation agent used in beverages, and the like. In addition, the composition of the present invention may contain the pulp for the production of natural fruit juice, fruit juice beverage, and vegetable beverage. These components may be used independently or in combination. Although the proportion of these additives is not very important, the composition of the present invention is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight.

In addition, the present invention is the bark of a birch tree; leaves of willow ash; a mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; It relates to a pharmaceutical composition for the prevention or treatment of any one bone disease selected from osteoporosis, osteopenia, osteomalacia, and osteogenic disorders, which contains an extract of any one of the genus oleracea as an active ingredient.

The composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent in addition to the active ingredient, and may be in various oral or parenteral formulations. In the case of formulation, it is prepared using commonly used diluents or excipients such as fillers, extenders, binders, wetting agents, disintegrants, and surfactants. Solid preparations for oral administration include capsules, powders, granules, tablets, pills, etc., and such solid preparations include one or more compounds and at least one excipient, for example, starch, calcium carbonate, sucrose or lactose ( lactose), gelatin, etc. In addition to simple excipients, lubricants such as magnesium stearate and talc are also used. Liquid formulations for oral administration include suspensions, emulsions, syrups, aerosols, etc., and various excipients such as wetting agents, sweetening agents, fragrances, preservatives, etc. in addition to commonly used simple diluents such as water and liquid paraffin. . Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, lyophilisates, and suppositories. As the non-aqueous solvent and the suspending solvent, propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate may be used. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycero gelatin, etc. may be used. For parenteral administration, it is preferable to select external or intraperitoneal, rectal, intravenous, intramuscular, subcutaneous, intrauterine dural or intracerebrovascular injection.

The pharmaceutical composition according to the present invention is administered in a pharmaceutically effective amount. In the present invention, "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable to medical treatment, and the level of the effective amount is determined by the type, severity, and drug activity of the patient. , can be determined according to factors including sensitivity to drug, administration time, administration route and excretion rate, duration of treatment, concurrent drugs, and other factors well known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, may be administered sequentially or simultaneously with conventional therapeutic agents, and may be administered singly or multiple times. In consideration of all of the above factors, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, which can be easily determined by those skilled in the art.

The dosage of the composition of the present invention varies depending on the patient's weight, age, sex, health status, diet, administration time, administration method, excretion rate, and severity of disease. The composition of the present invention can be used alone or in combination with methods using surgery, radiation therapy, hormone therapy, chemotherapy, and biological response modifiers.

Hereinafter, the present invention will be described in more detail using examples. These examples are only for illustrating the present invention in more detail, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited thereto.

Example 1. Preparation of extract of the sagittal plant

The extracts of the plants of the genus Sagitta were purchased from the Korea Plant Extracts Bank of the Korea Research Institute of Bioscience and Biotechnology. The plant names and extraction parts of the genus Saturn are shown in Table 1 below.

botanical name scientific name extraction site ash tree Euonymus alata for. ciliatodentatus outpost arrow tree Euonymus alatus stem leaf + stem stem (bark) branch leaf birch tree Euonymus sieboldiana Fruit stem leaf leaf + stem stem (bark) willow ash tree Euonymus trapococcus leaf stem birch tree Euonymus bungeanus leaf + stem fern tree Euonymus fortunei var. radicans outpost leaf + stem + flower leaf + stem leaf Branch fern tree Euonymus japonicu s stem leaf + stem leaf Branch Confetti Euonymus oxyphyllus leaf stem leaf + stem boxwood Euonymus pauciflorus leaf stem boxwood Euonymus sachalinensis leaf stem

Methanol was added to the powder sample dried and pulverized for each plant part in the shade, and using an ultrasonic extractor (SDN-900H, SD-ULTRASONIC CO., LTD) at room temperature 30 times (40KHz, 1500W, 15 minutes extraction-120 minutes) stop) and repeated extraction. Then, after filtration and concentration under reduced pressure, an extract was obtained, which was dissolved in dimethyl sulfoxide and used in the experiment. In Table 1, the stem refers to the entire stem, and the stem (bark) is obtained by separating only the bark from the stem.

Example 2. Analysis of the effect of extracts of the genus Sagittarius on osteoclast differentiation

The purpose of this study was to determine whether or not the extract of the genus Saturnum inhibits the differentiation of osteoclast progenitors into osteoclasts. The osteoclast progenitor cells were treated with the extract of the genus Arrica by concentration, and the number of osteoclasts was analyzed accordingly.

Specifically, bone marrow cells of mice were cultured in α-MEM medium containing M-CSF (60ng/ml) and 10% FBS for 3 days to obtain bone marrow cell-derived macrophages (BMM), which were then transformed into osteoclast progenitors. was used. To generate osteoclasts, 1×10 ⁴ cells of the obtained bone marrow cell-derived macrophages per well of a 96-well plate were placed in a medium containing M-CSF (60 ng/ml) and RANKL (50 ng/ml) for 4 days. Incubated for a while, the BMM was treated with the concentration of the extract of the genus Arachnoid (11.1, 33.3, 100 μg/ml) in the same culture condition. After fixing the cells with 10% formalin, TRAP (Tartrate-resistant acid phosphatase) staining was performed using naphthol AS-MS phosphate and Fast red violet LB salt, and TRAP-positive cells having three or more nuclei (osteoclasts, osteoclasts) were counted.

As a result, as shown in FIG. 1 , the osteoclast differentiation inhibitory effect was exhibited by the treatment with the plant extract of the genus Sagittary.

Example 3. Analysis of the effect of extracts of the sagittal plant on cell viability

The purpose of this study was to determine whether or not the extracts of the genus Arboraceae inhibited the survival of osteoclast progenitor cells.

Specifically, after putting 2 × 10 ⁴ bone marrow cell-derived macrophages (BMM) obtained in Example 2 per well of a 96-well plate, various concentrations for 1 day in a medium containing M-CSF (60 ng/ml) Incubated by adding a sample of the plant extract of the genus Saturnum. Cell viability was confirmed by measuring absorbance at 450 nm using a cell counting kit-8 (Cell Counting Kit-8, Dojindo Inc.).

As a result, as shown in FIG. 2 , cell viability was maintained or increased similar to that of the control group.

Example 4. Analysis of the effect of extracts of the genus Sagitta on osteoblast differentiation

The purpose of this study was to determine whether a plant extract of the genus Achilles plant promotes osteoblast differentiation. The mesenchymal stem cells were treated with extracts of the genus Arachnoid by concentration, and the degree of differentiation into osteoblasts was analyzed by Alizarin Red S staining.

Specifically, 1.7×10 ⁴ mouse bone marrow-derived mesenchymal stem cells (D1 ORL UVA) were added per well of a 96-well plate, and then cultured in DMEM medium containing 10% FBS for 2 days. After replacement with α-MEM medium containing 10% FBS, ascorbic acid (50㎍/㎖) and β-glycerophosphate (β-glycerophosphate, 5mM) were treated for 8 days to induce osteoblast differentiation, and the same In the culture conditions, the concentrations of the plant extracts of the genus A. genus were treated (11.1, 33.3, 100 μg/ml). Cells were fixed with 10% formalin and then stained with Alizarin red S to check the degree of calcification according to osteoblast differentiation. Alizarin red S stained with 10% acetic acid was extracted and the concentration was measured.

As a result, treatment with extracts of serrata (stem, leaf+stem, stem (bark), branch, leaf), cypress tree (stem, leaf+stem, stem (bark)) and willow tree (leaf) showed the osteoblast differentiation promoting effect.

Also perennials (outpost, leaf+stem+flower, leaf+stem, branch), evergreen (stem, leaf+stem, leaf, branch), Confetti (stem, leaf+stem), and ash tree (leaf, stem) It exhibited the osteoblast differentiation promoting effect by the extract treatment (FIG. 3).

[Statistics processing]

The data obtained in Examples 2 to 4 of the present invention were expressed as mean ± standard deviation, and statistical analysis was calculated by one-way ANOVA accompanied by Dunnett's test.

Claims (8)

the bark of a birch tree; leaves of willow ash; a mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; A health functional food composition for the prevention or improvement of any one bone disease selected from osteoporosis, osteopenia, osteomalacia, and bone formation disorders, comprising an extract of any one of the genus Arborus genus selected from among the extracts as an active ingredient. The health functional food composition for the prevention or improvement of bone disease according to claim 1, wherein the extraction solvent of the plant extract of the genus Arboraceae is water, C ₁ ~ C ₄ lower alcohol, or a mixture thereof. [Claim 2] The health functional food composition for preventing or improving bone disease according to claim 1, wherein the plant extract of the genus Sagittarius inhibits the differentiation of osteoclasts. [Claim 2] The health functional food composition for preventing or improving bone disease according to claim 1, wherein the plant extract of the genus Sagittarius promotes osteogenesis. The health functional food composition for preventing or improving bone disease according to claim 1, wherein the bone disease is caused by bone loss. According to claim 1, wherein the composition is a health functional food composition for the prevention or improvement of bone disease, characterized in that it is prepared in any one formulation selected from powder, granules, pills, tablets, capsules, candy, syrup and beverages. the bark of a birch tree; leaves of willow ash; a mixture of leaves, stems and flowers of the evergreen tree; a mixture of leaves and stems of the evergreen tree; a mixture of leaves and stems of perennials; Evergreen leaves; the trunk of an evergreen tree; and branches of fern trees; A pharmaceutical composition for the prevention or treatment of any one bone disease selected from osteoporosis, osteopenia, osteomalacia, and bone dysplasia, comprising an extract of any one of the arrowroot plants selected from among. The pharmaceutical composition for preventing or treating bone disease according to claim 7, further comprising a pharmaceutically acceptable carrier, excipient or diluent in addition to the active ingredient.

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