KR102407314B1 - Pippete for medium extraction - Google Patents

Abstract

The pipette for extracting a medium according to the present invention is a medium for extracting a medium in which a medium surrounding cells is accommodated in a vessel, and is disposed at a predetermined distance from the bottom of the vessel to extract the medium, and is inserted into the vessel and the A suction unit having a hole for sucking the medium, a moving unit extending from the suction unit to the upper portion of the container and serving as a passage through which the medium is moved, and a receiving unit connected to the moving unit to receive the medium sucked in the suction unit and a pressure providing unit coupled to the receiving unit to provide pressure so that the suction unit extracts the medium, the suction unit is disposed inside the container together with the moving unit, and the suction unit is bent at a preset angle to form the medium It provides a pipette for medium extraction, characterized in that for extracting.

Description

Pipette for medium extraction

The present invention relates to a pipette for extracting a medium, and more particularly, to a pipette for extracting a medium that extracts only the medium while preventing cell loss in order to extract the medium from a container in which the medium and cells or cell spheroids are accommodated.

Recently, various cells are cultured and used to predict changes that can occur in the body by preferentially applying them to cells prior to the interpretation of life phenomena and the application of toxic substances or drugs.

In order to culture the cells or cell spheroids as described above in vitro, the cells can be grown by locating the cells in a container containing a medium, which is a nutrient source in a liquid or gel state, in which the cells can grow.

However, since it is necessary to periodically replace the medium for cell culture or to remove the medium in order to observe the grown cells, it is necessary to go through the process of suctioning the medium using a pipette.

However, the pipette manufactured according to the prior art is a cause of stress and fatigue of researchers who want to observe this as the cells are lost or damaged as the cells are sucked together with the medium in which the cells are sucked while the medium is sucked.

In addition, even if the suction speed is adjusted to reduce cell movement, very small cell units can move even at a slow flow rate, so they are lost because they are moved along with the suctioned medium, or it is difficult to visually identify the location of the cells. In this case, there was a disadvantage in that the cells may be lost and separated from the site where they were located by the flow rate of the medium.

In addition, although suction power using a machine is used, when a person directly uses suction power with a pipette, it is difficult to control the suction power carefully, so there is a problem in that the cells are moved by the flow rate at which the medium is sucked and the cells are lost together with the suctioned medium. .

In order to overcome such disadvantages, the medium extraction pipette prevents the risk of cell loss or damage, regulates the flow rate of the suctioned medium, and prevents cells from being lost even when it is difficult to control the suction power. A means for maintaining the speed as low as possible is actively being devised, and a method for solving such problems is required.
[Prior literature]
China Utility Model Registration Publication 206121775 (2017.04.26.)

The present invention is an invention devised to solve the problems of the prior art described above, and since the suction part is bent by a predetermined angle toward the receiving part to prevent direct suction of the medium toward the cells, the loss of cells can be effectively prevented. In addition, a guard part is formed that can prevent the loss of cells together with the medium due to cell loss by the flow rate of the medium being sucked in, and the moving part through which the medium moves is formed narrower than the suction part to maintain the flow rate of the medium at a low speed. can have an effect.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

The pipette for medium extraction of the present invention for achieving the above object is a medium extraction pipette in which a medium surrounding cells is accommodated in a vessel, and is disposed at a predetermined distance from a bottom surface of the vessel to extract the medium, A suction part inserted into the container and having a hole for sucking the medium, a moving part extending from the suction part to the upper part of the container and becoming a passage through which the medium is moved, the moving part connected to the moving part and sucked in the suction part and a receiving unit for accommodating the medium and a pressure providing unit coupled to the receiving unit to provide pressure so that the suction unit extracts the medium, wherein the suction unit is disposed inside the container together with the moving unit, and the suction unit is It is characterized in that it is bent at a set angle to extract the medium.

Here, the suction unit, characterized in that for extracting the medium in the upper portion of the suction unit.

In addition, the suction unit is bent by 90 degrees toward the receiving unit, so that a direction in which the medium is sucked into the suction unit and a direction in which the medium is moved through the moving unit are perpendicular to each other.

On the other hand, when the medium is inhaled, it characterized in that it further comprises a guard for preventing the cells from being sucked together.

In addition, the guard unit is coupled to the suction unit, it is characterized in that a compartment is formed in contact with the bottom surface of the container to isolate the cells.

In addition, the guard portion, characterized in that the elastic member is provided in order to alleviate the noise and impact generated in the portion in contact with the bottom surface of the container.

On the other hand, the moving part, characterized in that the inner diameter is formed to be relatively narrower than the diameter of the suction part to maintain the flow rate of the medium sucked through the suction part at a low speed.

In addition, the moving part is characterized in that the center of the outer diameter and the inner diameter of the moving part is formed differently from each other.

In addition, the pressure providing unit, characterized in that the cylinder or suction pump.

The pipette for medium extraction of the present invention for solving the above problems has the following effects.

First, the suction unit is bent by a predetermined angle toward the receiving unit, so that the suction unit is positioned toward the cell, thereby more effectively preventing the risk of cell loss.

Second, when a guard portion extending from the suction unit toward the cells is formed and the suction unit sucks the medium, there is an advantage in that the cells can be prevented from being sucked together with the medium while moving along with the medium being sucked.

Third, the moving part through which the inhaled medium is moved is formed to be narrower than the suction unit, and the passage through which the inhaled medium is moved is formed narrow like a capillary tube, so that the flow rate at which the medium is sucked can be maintained at a low speed.

Effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

1 is a view showing the overall appearance of a pipette for extracting a medium according to an embodiment of the present invention;
2 is a view showing the suction part of the pipette for extracting the medium according to an embodiment of the present invention;
3 is a diagram illustrating a situation in which a pipette for extracting a medium according to an embodiment of the present invention is applied;
Figure 4 is a view showing a situation in which the medium extraction pipette according to an embodiment of the present invention inhaled the medium;
5 is a view illustrating a situation in which a preset angle of a pipette for extracting a medium according to an embodiment of the present invention is different;
6 is a diagram illustrating a situation in which a medium is sucked in a situation where a preset angle of a pipette for medium extraction according to an embodiment of the present invention is different;
7 is a view showing a guard portion of the pipette for extracting the medium according to an embodiment of the present invention;
8 is a view showing an elastic member of a pipette for extracting a medium according to an embodiment of the present invention;
9 is a view showing the radius of the moving part and the suction part of the pipette for extracting the medium according to an embodiment of the present invention; and
10 is a view showing the center of the outer diameter and the center of the inner diameter of the pipette for medium extraction according to an embodiment of the present invention.

Hereinafter, preferred embodiments of the present invention in which the object of the present invention can be specifically realized will be described with reference to the accompanying drawings. In describing the present embodiment, the same names and the same reference numerals are used for the same components, and an additional description thereof will be omitted.

First, a configuration of a pipette for extracting a medium according to an embodiment of the present invention will be described in detail with reference to FIGS. 1 to 2 .

Here, FIG. 1 is a view showing the overall appearance of a pipette for medium extraction according to an embodiment of the present invention, and FIG. 2 is a view showing the suction unit 100 of the pipette for medium extraction according to an embodiment of the present invention. to be.

Specifically, as shown in FIG. 1 , the pipette for medium extraction according to an embodiment of the present invention is composed of a suction unit 100 , a receiving unit 200 , and a moving unit 300 , and the moving unit 300 is It may include a medium passage 320 through which the medium (M) is moved.

In more detail, the pipette includes the suction unit 100 in which a hole for sucking the medium M is formed, and the receiving unit 200 in which the medium M sucked through the suction unit 100 is accommodated. and the moving unit 300 connecting the suction unit 100 and the receiving unit 200 and providing the medium moving path 320 through which the medium M is moved.

In addition, as shown in FIG. 2 , the suction unit 100 is bent toward the receiving unit 200 so that the medium M is sucked through the suction unit 100 and the medium M is separated. The moving directions may be different from each other.

Specifically, the suction part 100 is inserted into the container P, and the suction part 100 is disposed to be spaced apart from the bottom surface of the container to extract the medium M, and the receiving part 200 ), the direction in which the medium (M) is sucked through the suction unit 100 is when the cell (C) is located between the suction unit 100 and the container (P). The medium (M) is sucked toward the bottom surface of the container (P), and the medium (M) sucked through the suction unit 100 is where the container (P) is located through the moving part (300). It can move in the opposite direction to the direction, that is, toward the top of the container (P).

Here, the pipette for extracting the medium according to an embodiment of the present invention is provided with a pressure providing unit (not shown) that provides a suction force, so that the suction unit 100 extracts the medium (M) from the receiving unit 200 side. It can provide suction power for

For example, the pressure providing unit (not shown) may be provided as a cylinder or a suction pump to provide suction power through which the medium M may be suctioned from the suction unit 100 .

Therefore, it is formed so that the direction in which the medium M moves and the direction in which the medium M is sucked through the suction unit 100 are different from each other, so that the medium M can be extracted.

Specifically, in order to explain the effect of such a phenomenon, it may be described in more detail with reference to FIGS. 3 to 6 .

Here, FIG. 3 is a diagram illustrating a situation in which the medium extracting pipette according to an embodiment of the present invention extracts the medium (M), and FIG. 4 is a medium extraction pipette according to an embodiment of the present invention. (M) is a view showing the extraction situation, and FIG. 5 is the suction part 100 of the pipette for medium extraction according to an embodiment of the present invention toward the receiving part 200 by a preset angle θ. 6 is a view showing a bent state and FIG. 6 is a pipette for medium extraction according to an embodiment of the present invention, wherein the suction unit 100 is bent toward the receiving unit 200 by a preset angle θ It is a view showing a situation in which the medium (M) is extracted from the state.

First, as shown in FIG. 3, the suction unit 100 is inserted into the vessel P in which the cells (C) and the medium (M) are accommodated, and the suction unit 100 is connected to the vessel ( It is disposed at a predetermined distance from the bottom surface of P) to suck the medium (M).

Here, the cell (C) may refer to a single cell, or may be a multicellular cell spheroid, or may refer to a cell having a specific substrate or characteristic.

As such, when the medium M is sucked through the suction unit 100, the medium M equal to the height of the hole of the suction unit 100 and the container P remains as a residual amount, There is an effect of preventing the cells (C) from being lost through the suction unit (100) together with the remaining medium (M).

In addition, the suction unit 100 is bent toward the upper portion of the container (P) as shown in FIG. 3 to prevent direct contact with the cell (C), and the suction toward the cell (C) The part 100 is positioned so that it is possible to prevent the cells C from being sucked into the suction part 100 .

That is, in order to prevent the suction unit 100 from sucking the cells (C) together with the medium (M) by applying a suction force toward the cells (C), a direction different from the direction in which the cells (C) are located It is in the shape of being bent toward the upper part of the said container P.

In addition, since the suction part 100 faces the upper part of the container P, the suction part 100 extracts only the medium M in the upper part of the suction part 100 through the hole. can

In addition, in more detail, as shown in FIG. 4 , the suction unit 100 is bent toward the receiving unit 200 and the medium ( M) remains, and it is possible to prevent loss of the cells (C) by the remaining medium (M).

Specifically, when all of the medium (M) is extracted, the medium (M) flows around the cells (C) so that the cells (C) flow through the medium (M) by the flow of the medium (M). As the cells (C) are moved in accordance with the flow, there is a risk that the cells (C) are damaged or lost due to being introduced into the suction unit (100). There is an effect of preventing the cell (C) from being lost by the flow of the medium (M) around the cell (C) by remaining the medium (M) as high as the height.

Accordingly, in the process of removing the medium (M) in order to observe the cells (C) by a pipette for medium extraction manufactured according to the prior art, the cells (C) are removed by the suction force of the suction unit (100). Tension felt by researchers or users who want to observe the cell (C) due to damage that occurs when the cell (C) is lost when it flows into the suction unit 100 or the cell (C) moves into the pipette Although excessive stress and inconvenience occurred, the suction part 100 of the pipette for medium extraction of the present invention is bent toward the receiving part 200 to prevent direct suction force from acting toward the cell (C). It has the effect of significantly lowering the probability that the cell (C) is lost, thereby preventing tension, stress, and discomfort felt by the researcher or the user.

On the other hand, as shown in FIG. 5 , the suction unit 100 may be bent by a preset angle θ between the suction unit 100 and the moving unit 200 toward the receiving unit 200 . In addition, the preset angle θ may be variously changed according to the intention of a manufacturer who manufactures the pipette for medium extraction of the present invention or a user who requires the production.

Specifically, when the preset angle θ between the suction unit 100 and the moving unit 300 approaches 0 degrees indefinitely, the suction unit 100 moves toward the upper portion of the container P. The hole is directed to suck the medium M, and the medium M remains as much as the height between the hole of the container P and the suction unit 100, and the preset angle θ is It is a self-evident fact that the remaining amount of the medium (M) becomes smaller as it approaches 180 degrees.

However, when the preset angle θ becomes 180 degrees to generate suction force toward the cell C, the cell C, such as a pipette for medium extraction prepared according to the prior art, is lost. It is preferable to design the preset angle θ within 180 degrees because discomfort occurs, and as the preset angle approaches 0 degrees indefinitely, the probability that the cells C may be lost may be lowered.

On the other hand, since the remaining amount of the medium (M) decreases as the preset angle (θ) is toward 180 degrees, it is the most ideal to minimize the remaining amount of the medium (M) and the loss of the cells (C). The angle is a state in which the suction unit 100 is bent by 90 degrees toward the receiving unit 200 , and at this time, the direction in which the medium M is sucked into the suction unit 100 and the medium M A direction in which is moved toward the moving unit 300 may be vertical.

Specifically, the remaining amount of the medium (M) shown in FIG. 4 and the remaining amount of the medium (M) shown in FIG. 6 decrease as the preset angle (θ) approaches 180 degrees, so the most ideal 90 It is possible to minimize the amount of the remaining medium (M) while reducing the probability that the cells (C) may be lost by designing the preset angle (θ).

At this time, even though the suction unit 100 is bent by the preset angle θ, there is a probability that the cells C may be lost. As shown, the cell (C) may be formed to include a guard unit 400 for preventing the movement toward the suction unit (100).

Here, FIG. 7 is a view showing the guard part 400 of the pipette for extracting media according to an embodiment of the present invention, and FIG. 8 is an elastic member 420 of the pipette for extracting media according to an embodiment of the present invention. is a diagram showing

Specifically, to explain based on FIGS. 7 to 8 , first, as shown in FIG. 7 , the compartment is coupled to the suction unit 100 and is in contact with the bottom surface of the container P to isolate the cells C. The formed guard unit 400 is formed so that the cell (C) is inserted into the compartment of the guard unit 400 and isolated from the outside, and the suction unit 100 sucks the medium (M). In this case, the medium (M) in the compartment of the guard unit 400 cannot be moved toward the suction unit 100 to prevent the cells (C) from being lost by the flow rate of the medium (M). there may be an effect.

Here, the guard unit 400 blocks the cells (C) and the medium (M) from the inside to the outside of the guard unit 400 to prevent loss of the cells (C), The guard unit 400 may be formed in various ways different from that shown in FIG. 7 .

For example, as shown in FIG. 7 , the guard unit 400 may be made of a metal material, a glass material, or a plastic material to form a partition wall, and not a shape extending toward the bottom surface of the container (P). It may be formed widely around the suction unit 100, and the barrier ribs of the guard unit 400 form a network, so that the medium M flows, but the cells C pass through the barrier ribs of the guard unit 400. It is possible to prevent the cell (C) from being lost because it does not pass.

Therefore, the material and shape of the partition wall of the guard unit 400 may be variously changed, but the plastic extending tapered as the circumference increases toward the cell C in the suction unit 100 presented in the detailed description of the present invention Alternatively, the metal guard unit 400 effectively prevents damage to the cell (C) that occurs while moving toward the cell (C), and by the flow rate of the medium (M) while the cell (C) moves Loss can be effectively prevented.

Meanwhile, an elastic member 420 may be provided around the guard portion 400 where the guard portion 400 and the container P contact each other.

At this time, as described above, when the guard unit 400 is formed of a plastic or metal material, the container (P) and the guard unit 400 prevent noise generated by colliding, and the container (P) There is an effect that can alleviate the shock generated while the guard portion 400 is moved toward the.

Here, the elastic member 420 may be simply made of a rubber material, or may be made of a sponge material that does not absorb the medium (M) in order to prevent the cells (C) from being damaged, and the medium (M) ) may be provided with a sponge material that absorbs. This is only an example presented in order to better understand the present invention, and the material of the elastic member 420 is not necessarily limited to the presented example.

On the other hand, the guard unit 400 is coupled to the suction unit 100 to suppress the movement by isolating the cell (C) in the compartment and prevent the cell (C) from being lost, The movement of the cell (C) may be suppressed by maintaining the flow rate of the medium (M) at a low speed through the moving unit (300).

Specifically, it can be described in more detail through the drawings shown in FIGS. 9 to 10 .

Here, FIG. 9 is a view for comparing the radius of the suction unit 100 and the moving unit 300 of the pipette for extracting media according to an embodiment of the present invention, and FIG. 10 is an embodiment of the present invention. It is a view for comparing the moving part 300 and the passage 320 of the pipette for extracting the medium according to .

First, looking at the cut surface of the suction unit 100 and the moving unit 300 as shown in FIG. 9 , the suction unit 100 in the cross section of the suction unit 100 and the moving unit 300 is The radius R and the radius r of the moving part 300 may be different from each other.

For example, when the radius r of the moving unit 300 is narrower than the radius R of the suction unit 100 , the medium M introduced through the suction unit 100 is moved As it moves toward the part 300, the radius r of the moving part 300 is narrowed so that the speed at which the medium M moves through the suction unit 100 is lowered, and eventually the medium M ) is sucked through the suction unit 100 at the same flow rate as the flow rate of the medium M moved through the moving unit 300 .

In addition, since the radius R of the suction part 100 is larger than the radius r of the moving part 300, the amount of the medium M moved through the moving part 300 is limited due to the limit. The flow rate of the medium (M) flowing into the suction unit 100 is eventually slowed down, and as the flow rate of the medium (M) is slowed, the cells (C) are lost while moving along with the flow of the medium (M). can be effectively prevented.

If the radius r of the moving part 300 is formed to be very narrow, even if a separate suction force is not provided, the medium M naturally flows through the capillary phenomenon that occurs as the moving part 300 narrows. An effect of being able to move toward the receiving part 200 may occur.

As such, when the medium M is sucked through the capillary phenomenon that occurs as the radius r of the moving part 300 is narrowed, the flow rate of the medium M is sufficient to move the cells C. Since the flow rate of the cell (C) is not generated, it is possible to more effectively prevent the loss of the cell (C).

However, when the radius r of the moving part 300 is small enough to cause a capillary phenomenon, there may be a risk that the moving part 300 may be easily broken by an external force.

Therefore, as shown in FIG. 10 , the moving unit 300 may have an outer diameter constituting the exterior of the moving unit 300 and an inner diameter in which the passage 320 through which the medium M moves is formed. have.

Specifically, as the radius r of the moving part 300 becomes narrower, the risk of the moving part 300 being damaged by an external force increases, so that the medium M can be moved inside the moving part 300. The inner diameter of the passage 320 may be formed to be different from each other.

As such, when the passage 320 having an inner diameter formed to be narrower than the outer diameter is provided inside the moving part 300, the thickness of the moving part 300 is formed thick to prevent it from being easily broken by an external force. The passage 320 in which the inner diameter of the moving unit 300 is narrower than the diameter of the suction unit 100 in order to maintain the flow rate of the medium M in the moving unit 300 at a low speed. ) provided with an effect of maintaining the flow of the medium (M) at a low speed while preventing the moving part 300 from being easily broken by an external force.

At this time, as shown in FIG. 10 , the center (A) of the outer diameter of the moving part 300 and the center (B) of the inner diameter in which the passage 320 is formed may be formed at different positions, and the inner diameter The center (B) of the is formed adjacent to the suction unit 100, the medium (M) can shorten the movement distance through the passage (320).

In the detailed description of the present invention, the center (A) of the outer diameter and the center (B) of the inner diameter are formed differently, and the center (B) of the inner diameter is adjacent to the suction unit 100 and the passage 320 is What is shown to be arranged is to shorten the moving distance through which the medium (M) is moved to shorten the time to extract the medium (M), and the center (A) of the outer diameter and the center (B) of the inner diameter ) can be formed identically to each other, but this has the effect of shortening the time for extracting the medium (M) by shortening the moving distance through which the medium (M) is moved, as described above in the detailed description of the present invention It can be difficult to see.

As described above, preferred embodiments according to the present invention have been reviewed, and the fact that the present invention can be embodied in other specific forms without departing from the spirit or scope of the present invention other than the above-described embodiments is a fact having ordinary skill in the art. It is obvious to them. Therefore, the above-described embodiments are to be regarded as illustrative rather than restrictive, and accordingly, the present invention is not limited to the above description, but may be modified within the scope of the appended claims and their equivalents.

θ: preset angle
A: center of outer diameter
B: center of inner diameter
C: cell
M: Badge
P: courage
R: Radius of suction part
r: radius of moving part
100: suction unit
200: receptacle
300: moving unit
320: badge passage
400: guard part
420: elastic member

Claims (9)

As a medium for extracting the medium is accommodated inside the vessel, the medium surrounding the cell is arranged to be spaced apart from the bottom surface of the vessel to extract the medium,
a suction unit inserted into the container and having a hole for sucking the medium;
a moving part extending from the suction part to the upper part of the container and becoming a passage through which the medium is moved;
a receiving unit connected to the moving unit to receive the medium sucked from the suction unit;
a pressure providing unit coupled to the receiving unit to provide pressure to the suction unit to extract the medium; and
The suction unit is coupled to the suction unit so as to extend from the suction unit toward the bottom surface of the vessel, and a compartment is formed therein to contact the bottom surface of the vessel to prevent the cells from being sucked together while extracting the medium. Including a guard portion for isolating the cell from the outside,
The suction part,
It is disposed inside the container together with the moving part, and the suction part is bent at a preset angle to extract the medium,
Pipette for medium extraction.
According to claim 1,
The suction part,
Characterized in extracting the medium in the upper part of the suction unit,
Pipette for medium extraction.
3. The method of claim 2,
The suction part,
It is bent by 90 degrees toward the receiving unit, characterized in that the direction in which the medium is sucked into the suction unit and the direction in which the medium is moved through the moving unit are perpendicular to each other,
Pipette for medium extraction.
delete delete According to claim 1,
The guard unit,
Characterized in that an elastic member is provided to alleviate noise and shock generated in a portion in contact with the bottom surface of the container,
Pipette for medium extraction.
According to claim 1,
The moving unit,
Characterized in that the inner diameter is formed relatively narrower than the diameter of the suction part to maintain the flow rate of the medium sucked through the suction part at a low speed,
Pipette for medium extraction.
8. The method of claim 7,
The moving unit,
characterized in that the centers of the outer diameter and inner diameter of the moving part are formed differently from each other,
Pipette for medium extraction.
According to claim 1,
The pressure providing unit,
Characterized in that it is a pneumatic cylinder or a suction pump,
Pipette for medium extraction.

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