KR102398017B1 - Fermented red teatree, manufacturing method thereof, and use thereof - Google Patents

Abstract

The present invention relates to a red teatree fermented product, a method for preparing the same, and a use thereof and, more specifically, to a red teatree fermented product prepared by fermenting a red teatree extract with one or more microorganisms, a method for preparing the same, and a cosmetic composition, a quasi-drug composition, and a skin external application composition for soothing the skin or alleviating skin inflammation comprising the same. The red teatree fermented product has excellent anti-inflammatory activity, is safe by being prepared with natural ingredients and useful microorganisms and thus reducing toxicity or side effects, and can maximize the beneficial effects on the skin.

Description

Fermented red tea tree, manufacturing method thereof, and use thereof

The present invention relates to a fermented red tea tree, a method for preparing the same, and a use thereof, and more particularly, to a fermented red tea tree prepared by fermenting a red tea tree extract with one or more microorganisms, a method for preparing the same, and claims comprising the same It relates to a composition for inflammation.

Kefir is originally fermented effervescent milk often eaten in the mountainous regions of the Caucasus, and its name comes from the Turkish word kef. Commonly made from goat's milk, sheep's milk, etc. Similar to Eastern Europe's Urda (Urda) and Chile's Skuta (Skuta). As the fermenting flora, a spawn called Tibetan mushroom is usually used and contains 12 kinds of bacteria and yeast. In addition, wound healing and antibacterial effects have been reported.

Fermentation, in a broad sense, refers to the process of breeding using microorganisms or fungi, and in a narrow sense, refers to a metabolic process in which energy is obtained by decomposing sugar without oxygen. The product of fermentation is an organic acid or alcohol, and the strains mainly used in the cosmetic industry are lactic acid bacteria, yeast, Bacillus , Aspergillus , etc. The standards can be determined as presented in the Korean Cosmetic Ingredients Dictionary.

Recently, in order to reduce skin irritation caused by various chemical substances, a lot of attention has been paid to functional cosmetics having functions such as antioxidant, anti-inflammatory, and wrinkle relief obtained from natural products. In addition, natural fermented products prepared by using microorganisms beneficial to the human body without using organic solvents are safe without skin irritation by lowering the intrinsic toxicity of natural products, and have the advantage of maximizing the effective efficacy of the skin. As consumers' response to cosmetics using these natural materials increases, natural fermented products manufactured using natural materials and beneficial microorganisms are receiving more attention as their development value as cosmetic raw materials increases.

Red teatree ( Leptospermum ) scoparium ) is a plant called the Australian plum or manuka, derived from the plant's leaves being used as a tea substitute by early settlers in Australia. It is a semi-cold-resistant plant that grows up to 6m in height and has many branches. Flowers, leaves, and stems are usually used as essential oils. However, research on its fermented product is still insufficient.

Republic of Korea Patent Publication No. 10-2017-0065187 (published on June 13, 2017)

An object of the present invention is to provide a method for producing a fermented red tea tree having excellent anti-inflammatory activity.

Another object of the present invention is to provide a fermented red tea tree prepared by the above production method.

Another object of the present invention is to provide an anti-inflammatory composition comprising the fermented red tea tree.

In order to achieve the above object, the present invention comprises the steps of preparing a red tea tree (Leptospermum scoparium) extract; and fermenting the prepared extract with one or more microorganisms selected from the group consisting of lactic acid bacteria and yeast.

The present invention provides a fermented red tea tree prepared according to the above production method.

In addition, the present invention provides a cosmetic composition for preventing or improving skin inflammation, a quasi-drug composition, and a composition for external application for skin containing the fermented red tea tree as an active ingredient.

The red tea tree fermented product fermented by lactic acid bacteria and yeast according to the present invention has excellent anti-inflammatory activity, and can be used as a cosmetic composition, a quasi-drug composition, and a composition for external application for skin for skin soothing or skin inflammation improvement.

The composition is prepared by using natural materials and useful microorganisms, so that it is safe to reduce toxicity or side effects, and it is possible to maximize useful effects on the skin.

1 is a photograph in which lactic acid bacteria and yeast are separated according to an embodiment of the present invention.
2 is a nucleotide sequence of the strain isolated according to FIG. 1, the nucleotide sequence of Lactobacillus sacai NCK01 (Accession No.: KFCC11856P) and the nucleotide sequence of the yeast Kluyveromyces marcianus NCK02 (Accession number: KFCC11857P) it has been shown
3 is an experimental result confirming the cytotoxicity of the fermented red tea tree according to an experimental example of the present invention.
4 is a result of confirming the NO production inhibitory ability of the fermented red tea tree according to another experimental example of the present invention.
5 is a result of confirming the ability to inhibit the production of cytokines (IL-1β and IL-4) of the fermented red tea tree according to another experimental example of the present invention.

Hereinafter, the present invention will be described in detail.

The present inventors have prepared a red tea tree fermented product using self-isolated lactic acid bacteria and yeast, and completed the present invention by confirming its anti-inflammatory activity.

The present invention provides a method for producing a fermented red tea tree.

The manufacturing method is a red tea tree ( Leptospermum scoparium ) preparing an extract; and fermenting the prepared extract with one or more microorganisms selected from the group consisting of lactic acid bacteria and yeast.

As used herein, the term "extract" refers to a substance obtained by extracting a component of a natural product regardless of an extraction method, an extraction solvent, an extracted component, or the form of an extract. It may include any material obtainable by processing or processing by the method.

The extract may be extracted according to a method commonly used in the art, for example, hot water extraction method, ultrasonic extraction method, filtration method, reflux extraction method, etc., these are performed alone or in combination of two or more methods. can be

In the method for producing a fermented red tea tree according to the present invention, the step of preparing the red tea tree extract comprises extracting the red tea tree with an extraction solvent selected from water, C1 to C4 alcohol, or a mixed solution thereof to obtain a red tea tree extract. manufacturing; preparing a mixture by mixing the red tea tree extract extracted with the extraction solvent and ethyl acetate (EA) in a weight ratio of 1: (0.5 to 2); and separating the ethyl acetate layer from the prepared mixture.

Preferably, the red tea tree extract extracted with the extraction solvent may be an ethanol extract of red tea tree extracted with ethanol or an aqueous ethanol solution, more preferably the red tea tree is 30 to 70% by weight of an aqueous ethanol solution, most preferably may be an extract extracted with a 50% by weight aqueous ethanol solution, but is not limited thereto.

The mixture may be a mixture of the prepared red tea tree ethanol extract and ethyl acetate (EA) in a weight ratio of 1: (0.5 to 2), preferably 1:1, but is not limited thereto.

According to an embodiment of the present invention, after extracting the washed red tea tree raw material with a 50% by weight aqueous ethanol solution, it is mixed with ethyl acetate in a ratio of 1:1 in a separatory funnel, and then when the two solutions are sufficiently separated After waiting until the ethyl acetate layer was separated, it was concentrated and lyophilized to obtain a red tea tree extract.

In the method for producing a fermented red tea tree according to the present invention, the fermenting step may be performed as a single fermentation using a single microorganism, and may be performed as two or more stages of complex fermentation using two or more different microorganisms simultaneously or sequentially. can

Preferably, after primary fermentation with lactic acid bacteria to the prepared extract, secondary fermentation with yeast may be performed, but is not limited thereto.

The lactic acid bacteria are Lactobacillus genus ( Lactobacillus sp . ) or Leuconostoc sp . It may be selected from strains, and the yeast is Kluyveromyces sp . ) or Saccharomyces genus ( Saccharomyces ). sp . ) may be selected from the strain, but is not limited thereto.

Preferably, the lactic acid bacterium is Lactobacillus sakei NCK01 (Accession number: KFCC11856P), and the yeast is Kluyveromyces NCK02 ( Kluyveromyces marxianus NCK02) (Accession No.: KFCC11857P), but is not limited thereto.

The strain may be directly isolated or may be used after being sold.

The present invention provides a fermented red tea tree prepared according to the above production method.

Since the fermented product has excellent anti-inflammatory activity, it can be utilized as an anti-inflammatory composition. Specifically, it may be used as a cosmetic composition for skin soothing or prevention or improvement of skin inflammation, a quasi-drug composition, and a composition for external application to the skin, but is not limited thereto.

The present invention provides a cosmetic composition for preventing or improving skin inflammation containing a fermented red tea tree as an active ingredient.

The red tea tree fermented product has excellent anti-inflammatory activity, which can help to soothe the skin or improve skin inflammation.

As used herein, "inflammation improvement or anti-inflammation" means suppressing inflammation, and the inflammation is one of the body's defense responses to certain stimuli, and is related to skin diseases such as atopic dermatitis, acne, and eczema. It may have an improving effect of such skin diseases through an inflammatory improving effect.

The red tea tree fermented product may be included in an amount of 0.01 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition, but is not limited thereto.

The cosmetic composition according to the present invention may further include one or more of the ingredients listed in the list of cosmetic raw materials registered with the Ministry of Food and Drug Safety and ICID (International Cosmetic Ingredients). For example, the cosmetic composition may include an organic solvent, a solubilizer, a thickener, a gelling agent, an emollient, an antioxidant, a suspending agent, a stabilizer, a fragrance, a surfactant, an emulsifier, a filler, a sequestering agent, a preservative, a vitamin, a blocking agent, It may further comprise one or more adjuvants commonly used in the cosmetic field, such as wetting agents, dyes and pigments, hydrophilic or lipophilic actives, or any other ingredients commonly used in cosmetics.

The vitamin may include a water-soluble or oil-soluble vitamin, and the water-soluble vitamin is a water-soluble vitamin that can be formulated in cosmetics, preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, and nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like, and more preferably vitamin C, which helps collagen synthesis. In addition, salts thereof (thiamine hydrochloride, sodium ascorbate salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) may be included, and the water-soluble vitamin is a microorganism transformation method , can be obtained by a conventional method such as a purification method from a culture of a microorganism, an enzymatic method or a chemical synthesis method.

The cosmetic composition according to the present invention may be prepared in any formulation conventionally prepared in the technical field to which the present invention pertains. For example, it may be formulated as a lotion, emulsion, lotion, cream, paste, gel, solution, suspension, oil, wax, pack, powder, foundation, spray, surfactant-containing cleansing, etc., but is not limited thereto. .

More specifically, flexible lotion, nourishing lotion, astringent lotion, nourishing cream, massage cream, milk lotion, powder, essence, eye cream, sun lotion, sun cream, makeup primer, makeup base, BB cream, powder foundation, emulsion foundation , cleansing cream, cleansing foam, cleansing water, soap, pack, stick product, balm type product, spray or powder formulation.

When the cosmetic composition according to the present invention is a cream or gel formulation, animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be further included as a carrier component. can

When the cosmetic composition is a solution or emulsion formulation, a solvent, solvating agent or emulsifying agent, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid It may further include esters and the like.

When the cosmetic composition is a suspension formulation, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be further included.

When the cosmetic composition is in a powder or spray formulation, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be further included as a carrier component, and in particular, in the case of a spray formulation, additional chlorofluorohydrocarbon , may further include a propellant such as propane/butane or dimethyl ether.

When the cosmetic composition is a surfactant-containing cleansing formulation, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glycerides, fatty acid diethanolamide, vegetable oil, linoline derivatives or ethoxylated glycerol fatty acid esters may be further included.

The cosmetic composition according to the present invention may be applied alone or in overlapping use, or may be used by overlapping application with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention can be used according to a conventional method of use, and the number of times of use can be changed according to the skin condition or taste of the user.

The present invention provides a quasi-drug composition for preventing or improving skin inflammation containing a fermented red tea tree as an active ingredient.

As used herein, the term "quasi-drugs" refers to items with a milder action than pharmaceuticals among items used for the purpose of diagnosing, treating, improving, alleviating, treating or preventing diseases of humans or animals. For example, according to the Pharmaceutical Affairs Act, quasi-drugs Textiles and rubber products used for the treatment or prevention of diseases in humans and animals, which do not act directly on the human body, are not instruments or machines, and are similar to those that are not used for pharmaceutical purposes. This includes disinfectants and pesticides to prevent The type or formulation of the quasi-drug composition of the present invention is not particularly limited, but may be, for example, disinfectant cleaner, shower foam, gargrin, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment or filter filler.

When the composition of the present invention is used as a quasi-drug composition, the red tea tree fermented product may be added as it is or may be appropriately used in combination with other quasi-drug ingredients according to a conventional method. The mixing amount of the active ingredient may be appropriately determined according to the purpose of use.

The contents of the cosmetic composition may be applied mutatis mutandis to the quasi-drug composition of the present invention.

In addition, the present invention provides a composition for external application for skin for preventing or improving skin inflammation, comprising a fermented red tea tree as an active ingredient.

The composition for external application for skin may include, for example, ointment, lotion, solubilization, suspension, emulsion, cream, gel, spray, patch, warning agent, patch or water paste, but is not limited thereto. It may be formulated in any known base.

With respect to the composition for external application for skin of the present invention, the contents of the cosmetic composition or quasi-drug composition may be applied mutatis mutandis.

Hereinafter, examples will be described in detail to help the understanding of the present invention. However, the following examples are merely illustrative of the content of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.

< Example 1> red tea tree fermented product Produce

1. Preparation of raw materials

Red teatree ( Leptospermum ) scoparium ) was used by purchasing Australian plum bonsai through Naver shopping since there is no place that sells leaves separately in Korea.

2. Preparation of strains

To isolate lactic acid bacteria with useful functions, first, kefir yogurt samples were diluted to 10 ^-2 , 10 ^-3 , 10 ^-4 , 10 ^-5 and 10 ^-6 by decimal dilution, and then BL medium [proteose Proteose peptone No.3 10g/L, Beef extract 10g/L, Yeast extract 5g/L, Dextrose 20g/L, Polysorbate 80 1g/L L, Ammonium citrate 2g/L, Sodium acetate 5g/L, Magnesium sulfate 0.1g/L, Manganese sulfate 0.05g/L, Dipotassium phosphate ) 2g/L, agar 15.0g/L, Difco, USA], and SDB medium (Peptic Digest of Animal Tissue 5g/L, Pancreatic Digest of Casein 5g/L, Dextrose 20g/L, Difco, USA) The yeast was isolated by smearing, and colonies forming a ring around were isolated as candidate groups of the lactic acid bacteria genus.

3. 16s of the selected strain rRNA sequencing

The lactic acid strains grown in MRS medium were analyzed for nucleotide sequences by performing 16s rRNA sequence analysis. From the gDNA obtained by chromosomal DNA extraction, PCR was performed using the universal primers 27F (5' AGA GTT TGA TCM TGG CTC AG 3') and 1492R (5' TAC GGY TAC CTT GTT ACG ACTT 3'). The sequence was analyzed.

Yeast grown in SDA medium was subjected to ITS sequence analysis to analyze the nucleotide sequence. Based on the results obtained using the universal primers ITS1 (5'TCC GTA GGT GAA CCT GCG G 3') and ITS4 (5'TCC TCC GCT TAT TGA TAT GC 3') from gDNA obtained through chromosomal DNA extraction, The homology test was performed through BLAST search provided by NCBI.

Table 1 below is a list of primers used in this Example.

primer order 27F 5' AGA GTT TGA TCM TGG CTC AG 3' 1492R 5' TAC GGY TAC CTT GTT ACG ACTT 3' ITS1 5’TCC GTA GGT GAA CCT GCG G 3’ ITS4 5’TCC TCC GCT TAT TGA TAT GC 3’

As a result, Lactobacillus ( Lactobacillus ) belonging to the genus Lactobacillus sakei ( Lactobacillus sakei ) and showed 99.96% similarity and Kluyveromyces marxianus ( Kluyveromyces marxianus ) and 99.69% similarity.

- [Accession No. KFCC11856P] Lactobacillus sakei NCK01 ( Lactobacillus sakei NCK01)

- [Accession No. KFCC11857P] Kluyveromyces marxianus NCK02

4. Extraction Preparation

After removing soil and dust in running water, extract in 50% ethanol at 4 to 8℃ for 1 to 5 days, and mix ethylacetate (EA) with 50% ethanol extract 1:1 using a separatory funnel. Afterwards, I waited overnight until they were sufficiently separated. Only the separated EA layer was separated, concentrated, and freeze-dried.

5. Through strain fermentation fermented product Produce

1) Single-Strain Fermentation

As the lactic acid bacteria, Lactobacillus Sakei NCK01 (Accession No.: KFCC11856P) was used, and first, pre-culture was performed for 2-3 days using MRS broth (DB) at 30-37°C under anaerobic conditions. Yeast extract 5g/L, hydrolyzed soy peptone (HSP) 10g/L, dextrose 20/L, sodium acetate 1g/L and sodium chloride 5g/L to prepare a medium, and freeze-dried red tea tree After sterilization by inoculating the powder at about 1 to 3%, the pre-cultured strain was added and cultured at 30 to 37° C. for 3 to 7 days.

The yeast Kluyveromyces marcianus NCK02 (Accession No.: KFCC11857P) was used, and the pre-culture was performed for 3-7 days using YM broth (DB) at 20-27°C first. Prepare a medium by adding yeast extract 20g/L, hydrolyzed soy peptone (HSP) 5g/L, and dextrose 10/L, and inoculate 1-3% freeze-dried powder of red tea tree to sterilize and then pre-culture One strain was added and cultured at 20 to 27°C for 7 to 10 days.

2) Complex strain fermentation (two-stage fermentation)

In the case of complex fermentation with the two strains, the composition and temperature of the medium used for culturing a single strain were conducted under the same conditions.

(1) In the case of secondary Kluyveromyces fermentation after primary Lactobacillus fermentation, (2) In case of secondary Lactobacillus fermentation after primary Kluyveromyces fermentation, culture conditions and efficacy test results such as temperature 20 ~ 40℃ , the complex fermentation conditions of (1) were selected, and the detailed fermentation conditions were the same as the single strain fermentation conditions.

< Experimental example 1> Check for cytotoxicity: WST assay

In order to confirm that the red tea tree fermented product (single strain fermentation and two-stage fermentation) prepared according to Example 1 can exhibit an immune activation effect without stimulation such as exacerbation of inflammation, macrophages (RAW 264.7, ATCC) Toxicity was measured.

The fermented product was treated by concentration and after 48 hours, 10% EZ-Cytox (Dail, 99-EZ3000) was mixed in DMEM medium, and the sample-treated medium was dispensed by 100 μL per well after suction. After incubation for 2 hours, absorbance was measured at a wavelength of 450 nm with a microplate reader (Biotek, Synergy HT). Cell viability was calculated by comparing the sample with the untreated group (untreated group). same.

<Equation 1>

Cell viability (%) = (absorbance in the sample treated group / absorbance in the untreated group) * 100

As a result, as shown in FIG. 3, in the case of a two-stage fermented product of Red Tea Tree, a single-strain fermented product of Red Tea Tree Lactobacillus, and a single-strain fermented product of Red Tea Tree Kluyveromyces, cytotoxicity was not observed in all cells, and cosmetics It was confirmed that it can be used as a safe material for

< Experimental example 2> Check anti-inflammatory activity

1. NO Analysis

RAW 264.7, a mouse macrophage, was dispensed in a 96-well plate at a concentration of 5×10 ⁵ cells/mL, and cultured in a 5% CO ₂ incubator for 24 hours. After that, the cells RAW 264.7 were treated with 1.5 μg/mL of LPS (Sigma, St. Louis, MOLogan, UT, USA), and the red tea tree two-stage fermented product or single strain fermented product was treated by concentration and cultured for 24 hours. .

Next, the supernatant of the culture medium and a grease reagent (Griess reagent, Sigma) were reacted in the same amount, absorbance was measured at 540 nm with an ELISA reader, and the nitric oxide production rate was converted into a percentage. Nitrate ions (NO ₂ ⁻ ) were measured using nitrite as a standard solution. After treatment with 50 μM of L-NMMA, which is known to have anti-inflammatory effects, as a positive control, NO ₂ ⁻ production concentrations were compared.

As a result, as shown in FIG. 4 , in the case of the red tea tree single strain fermented product, NO production was 26.00% and 18.64% when 4% treatment was performed, whereas in the case of the red tea tree double-stage fermented product, NO production was 15.03 when treated with 4%. % was shown. Therefore, it can be confirmed that the anti-inflammatory effect of the two-stage fermented product compared to the single strain fermented product is enhanced.

2. IL- 1β & IL-4 Assay

Macrophages RAW264.7 were counted using a hemocytometer, and then aliquoted at a concentration of 2×10 ⁵ cells/well in a 24-well plate.

After culturing in DMEM medium (10% fetal bovine serum, 5% penicillin/streptomycin) for 24 hours, LPS (L2630, sigma) 1μg/mL and red tea tree double fermented product, red tea tree single strain fermented product and L-NMMA (M7033, sigma) were mixed with DMEM medium and treated simultaneously. Red tea tree two-stage fermented product or single strain fermented product was treated with 0 to 4%, and L-NMMA was treated with 100 nM as a positive control. After 20 hours of sample treatment, the culture medium was recovered and IL-1b ELISA (MLB00C, R&D systems) and IL-4 ELISA (M4000B, R&D systems) were performed. For IL-1b ELISA and IL-4 ELISA, protocols provided by the manufacturer were used.

As a result, as shown in Figure 5, in the case of a single fermentation of Red Tea Tree / Lactobacillus, it was reduced to 71.11% (IL-1β), 76.54% (IL-4) compared to the control (CTL) when treated with up to 4%, red Tea tree / Kluyveromyces single ferment was reduced to 68.11% (IL-1β) and 72.22% (IL-4) compared to CTL when treated with up to 4%. In addition, in the case of red tea tree/2-stage fermented product, IL-1β was reduced by up to 8% compared to single fermentation, with 63.49% (IL-1β) and 71.24% (IL-4), while IL-4 decreased by about 1% was shown to be effective.

< production example 1> Lactobacillus sake (Accession number: KFCC11856P ) and Kluyveromyces Marcianus (Accession number: KFCC11857P ) using bacteria red tea tree fermented contain manufacture of cosmetics

(1) manufacture of lotion

A lotion was prepared by a conventional method using the ingredients in the content shown in Table 2 below.

ingredient content (wt%) Red Tea Tree Fermented Product 3.00 Hydroxyethylene cellulose (2% aqueous solution) 12.00 Xanthan Gum (2% aqueous solution) 2.00 1,3-butylene glycol 6.00 glycerin 4.00 Sodium hyaluronate (1% aqueous solution) 5.00 Purified water 68.00 Sum 100.00

(2) lotion manufacturing

A lotion was prepared by a conventional method by applying the ingredients in the content shown in Table 3 below.

ingredient content (wt%) Red Tea Tree Fermented Product 3.00 Ascorbic acid-2-phosphate magnesium salt 1.00 Water-soluble collagen (1% aqueous solution) 1.00 sodium citrate 0.01 citric acid 0.05 1,3-butylene glycol 3.00 Purified water 91.94 Sum 100

As the specific parts of the present invention have been described in detail above, for those of ordinary skill in the art, it is clear that these specific descriptions are only preferred embodiments, and the scope of the present invention is not limited thereby. Do. That is, the substantial scope of the present invention is defined by the appended claims and their equivalents.

Korea Microorganism Conservation Center (domestic) KFCC11856P 20200409 Korea Microorganism Conservation Center (domestic) KFCC11857P 20200409

Claims (10)

Red tea tree ( Leptospermum scoparium ) Preparing an extract; and
Including; fermenting the prepared extract with one or more microorganisms selected from the group consisting of lactic acid bacteria and yeast;
The fermenting step is
It is performed by primary fermentation with lactic acid bacteria on the red tea tree extract prepared above, and secondary fermentation with yeast,
The lactic acid bacteria is Lactobacillus sakei NCK01 ( Lactobacillus sakei NCK01), and the yeast is Kluyveromyces marxianus NCK02 ( Kluyveromyces marxianus NCK02), characterized in that the red tea tree fermented product manufacturing method. The method of claim 1,
The step of preparing the red tea tree extract,
Preparing an ethanol extract of red tea tree by extracting the red tea tree with ethanol or an aqueous ethanol solution;
preparing a mixture by mixing the prepared red tea tree ethanol extract and ethyl acetate (EA) in a weight ratio of 1: (0.5 to 2); and
Separating the ethyl acetate layer from the prepared mixture; characterized in that it comprises a, manufacturing method. 3. The method of claim 2,
The red tea tree ethanol extract,
A manufacturing method, characterized in that the red tea tree is extracted with 30 to 70% by weight of an aqueous ethanol solution. delete delete A fermented red tea tree prepared according to any one of claims 1 to 3. 7. The method of claim 6,
The red tea tree fermented product,
Red tea tree fermented product, characterized in that it has anti-inflammatory activity. A cosmetic composition for preventing or improving skin inflammation comprising the red tea tree fermented product according to claim 6 as an active ingredient. A quasi-drug composition for preventing or improving skin inflammation, comprising the red tea tree fermented product according to claim 6 as an active ingredient. An external composition for skin application for preventing or improving skin inflammation, comprising the red tea tree fermented product according to claim 6 as an active ingredient.

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