KR102396164B1 - Cultivation method of soybean sprouts by using illite powder - Google Patents

Abstract

The present invention relates to a method for cultivating high-quality functional soybean sprouts by technically applying illite, paulownia leaves, and persimmon juice which are eco-friendly natural materials to a cultivating step when cultivating the soybean sprouts which are a traditional germinated food. More specifically, the method comprises: a material (soybean) sorting step as a first step; a soybean cleaning step as a second step; a first soaking step as a third step; a first cultivating step as a fourth step; a second soaking step as a fifth step; a second cultivating step as a sixth step; and a cleaning, packing, and product step as a seventh step. In the steps, by technically applying illite, paulownia leaves, and persimmon juice, the present invention provides high-quality soybean sprouts, which are technically cultivated and have a food value and functionality by finally receiving influence of beneficial ingredients of each additive material, and thus is beneficial to health of modern people and provides economic benefits to producers.

Description

Bean sprouts cultivation method using illite and the bean sprouts {Cultivation method of soybean sprouts by using illite powder}

The present invention is a traditional germinated food that can be easily grown at home regardless of the season and grafted bean sprouts, known as a major source of vitamin C in winter, to cultivation using eco-friendly natural materials illite, paulownia leaves and persimmon juice Thus, it relates to a method of cultivating high-quality functional bean sprouts.

Bean sprouts are called dua (豆芽) and sukahchae (菽牙菜), and by cultivating soybeans, which are raw materials, with water under suitable conditions, changes in components during sprouting cause changes in functional substances such as vitamin C and dietary fiber. It contains a lot of enzymes such as crease, urease, amidase, and amylase. In oriental medicine, dried bean sprouts are called soybean yellow spheres and are known to be used to relieve edema, muscle pain, and stomach heat.

Illite was first discovered in Illinois in the United States, and it is known in Korea that it was mass-buried in Yeongdong, North Chungcheong Province. This mineral belongs to the mica family belonging to the monoclinic system, and the streak color is white. Its effects include deodorizing effect, antibacterial effect, anion generation, odor neutralization effect, and harmful substance reduction effect.

Paulownia is a deciduous tree belonging to the genus Paulownia in the Hyeonsam family (玄蔘科), and although it has a similar appearance to the Paulownia tree, it is distinguished from the Paulownia tree by distinguishing it in characteristics. It is about 15m in height, and the bark is called Dongpi, leaves are called east, fruits are plump, and flowers are plump. In oriental medicine, the leaves of the paulownia tree are used for facial flushing, papules (acne-like condition), and pustules, and the fruits and flowers are known to be used for hemorrhoids, bruises, and malignant boils.

The origin of persimmon is Korea, China and Japan, and the cultivation history of Korea is during the Goryeo Myeongjong period. As for nutrients, sugar is about 14-16%, organic acid is about 0.1%, pectin is about 1.3-1.8%, and it has an astringent taste. It is known that about 0.39 to 0.77% of tannin, and about 800mg% of vitamin C, especially persimmon leaves.

Looking at the patent technology related to the conventional method for producing bean sprouts, Patent No. 10-2001-0043893''Cultivation method of bean sprouts using elm extract' Application No. 10-2012-0065497''Bean sprouts and cultivation method using cypress' Application No. 10-2001-0029965 ''Bean sprouts and cultivation method using herbal medicine' Registration No. 10-1381994''Cultivation method of bean sprouts using Sangha mushroom' Registration No. 10-1427169'' 'Registration No. 10-905107'' Bean Sprouts Continuous Production Equipment 'Registration No. 10-1446117'' Bean Sprouts Cultivation Container with Adjustable Length of Bean Sprouts 'Registration No. 10-1805432'' Growing Bean Sprouts Using Mulberry Extract Apparatus and its cultivation method', etc.;

As a patent technology related to illite, Publication No. 10-2021-0014960 ''Cosmetic composition containing wood flower and illite as active ingredients' Publication No. 10-2021-0085003'' Myrrh and illite are effective Cosmetic composition containing as ingredients 'Registration No. 10-2232460'' Fruit and vegetable freshness maintenance film containing Illite and manufacturing method thereof' Registration No. 10-1264530'' Highly moisturizing with Illite complex functional powder Powder-type color cosmetic composition 'Registration No. 10-1332268', 'Cosmeceutical composition containing illite and its manufacturing method', etc.

As for the patent technology related to paulownia leaves, application number 10-1986-0007279 'method for manufacturing cosmetics containing paulownia leaf extract 'application number 10-2006-0093298' 'cosmetic composition containing paulownia leaf extract', etc. there is,

Patent technology for persimmon includes registration number 10-0781763''Persimmon liquor using Monascus perprius red yeast strain and its manufacturing method 'Registration number 10-1804798''Rotary persimmon peeling device for automated fruit peeling 'Registration No. 10-2052017''Fruit jam containing persimmon concentrate and manufacturing method thereof' Registration No. 10-1372381''Persimmon transfer device for automated fruit peeling 'Registration No. 10-1150295''Persimmon with added persimmon Makgeolli manufacturing method 'Registration No. 10-1170278' and 'Persimmon makgeolli manufacturing method using persimmon' have been applied for and registered.

As can be seen, in the bean sprouts cultivation method, there has been no reported case of a technique for cultivating high-quality functional bean sprouts by grafting the above-mentioned illite, paulownia leaf and persimmon juice to cultivation.

Bean sprouts, the traditional germinated food of the nation, are undergoing major changes, such as mass production in response to changes in modern people's diet and increased demand. In particular, functional and eco-friendly bean sprouts are being developed in consideration of nutritional and functional aspects. Therefore, the present invention develops an eco-friendly bean sprouts cultivation method by grafting materials known as natural substances and eco-friendly materials when growing bean sprouts, thereby providing a functional and eco-friendly bean sprouts cultivation method that can achieve modern people's preference, health and high added value. As doing

The present invention invented a method of cultivating bean sprouts using natural materials illite, paulownia leaves and persimmon juice for cultivating functional bean sprouts that satisfy both consumers and producers when producing functional bean sprouts as a technical solution.

Accordingly, the present invention uses illite, paulownia leaf, and persimmon juice, which are eco-friendly natural materials, to cultivate high-quality functional bean sprouts by grafting it to the cultivation of high-quality functional bean sprouts. Used to wash raw soybeans, use illite immersion water for soaking raw beans, and illite cultivated water and pre-treated water for primary and primary cultivation It was cultivated using paulownia leaves. In the final stage, when cleaning the packaging of the cultivated bean sprouts, it was attempted to solve the technical problem by cultivating illite bean sprouts using a mixture of illite and persimmon juice.

By technically grafting illite, which is widely known and proven to contain scientifically beneficial ingredients, persimmon juice and paulownia leaf, which are beneficial as natural ingredients, when cultivating bean sprouts, the traditional sprouted food, bean sprouts, is finally cultivated to satisfy the tastes and preferences of modern people. By being able to provide eco-friendly and functional bean sprouts containing high quality and useful ingredients that meet the needs, it is possible to improve the health of the people and achieve high value-added of our agricultural products, thereby increasing consumer health and producer profits. There is an effect that can contribute.

1 is a flowchart showing a preferred embodiment of the present invention;

The present invention will be described in detail with reference to the accompanying drawings as follows.

Hereinafter, FIG. 1 is a process diagram of a bean sprouts cultivation method using illite, which will be described in detail as follows.

(1) Selection step of ingredients (beans)

This step is the screening step of the material (beans).

The raw soybeans used were sampled from Junjeori (variety) with an individual weight of 120±10 mg produced in Sangju, Gyeongbuk in 2020, and were used while storing at 4±℃ by selecting those with no abnormality in appearance.

(2) washing step of beans

In this step, 1 kg of the material (beans) selected in (1) is washed 3 times in tap water, put in a pot, and the raw soybeans minus 10 minutes of water are mixed with 10 liters of bedrock water 5% by weight of Illite powder (Y-1000, Medex, Korea) After washing the raw beans 3 times with water thoroughly mixed with stirring for 10 minutes, they were left at room temperature for about 1 to 2 hours, and finally washed 3 times with tap water.

(3) 1st soaking step

This step is the first soaking step of the raw soybeans washed in (2). First, 1 kg of washed beans was immersed in 3 liters of illite-containing water at 23±2° C. for 5 to 8 hours. That is, the illite-containing water was used as the immersion water for immersing the raw beans, and the illite-containing water was Y-1000 illite in 3 liters of bottled water (Illite, Yeongdong, Chungbuk produced by Medex) (illite)) Mix in the range of 0.5 to 5% by weight and use a stirrer (Daehan, TEC-121, Korea) at 200-300 rotations per minute for 1 hour mixing at 60-70°C for 1 hour, 40-45 A mixture of 2 hours at ℃ and 2 hours at 20-25 ℃ was used as illite-containing water.

(4) Primary cultivation stage

This stage is the primary cultivation stage of raw soybeans that have undergone the primary immersion stage in (3). As for the cultivation method, 1 kg of raw soybeans were cultivated at a water immersion temperature of 22±℃, watering frequency 9 times a day, watering time for 2 minutes, and at a cultivation temperature of 22±℃ for 1-2 days (germination length: about 0.5-1cm). The cultivated water used at this time was prepared by the same method as the illite-containing water in (3) above, and was used for watering (watering) using the illite cultivation water.

(5) secondary soaking (soaking) step

In this step, 1 kg of the primary cultivated bean sprouts (about 0.5 to 1 cm) grown in (4) are dried in the shade using a plastic immersion container: water containing illite is 2 : 1 (w/w) was mixed at a ratio of 20 to 25 °C and immersed for 2 hours at a secondary immersion (soaking) step. The illite immersion water used at this time was prepared in the same way as the illite-containing water in (3) above as the illite immersion water.

(6) Secondary cultivation stage

In this step, lay 1 to 4 pre-treated paulownia leaves on the bottom of the container (height × bottom diameter = 27 cm × 22 cm), pour the bean sprouts that went through step (5) on top, and black After covering with a cloth, the number of watering 9 times a day, watering time of 2 minutes, and cultivation temperature of 22±℃ were cultivated for 4 to 5 days.

The illite cultivation water used at this time was prepared in the same way as the illite-containing water in (3) above, and the illite cultivation water was used for watering (watering).

Meanwhile, the used pre-treated paulownia leaves are collected from fresh paulownia leaves from Yeongdong, Chungcheongbuk-do, washed in tap water, drained, and then cut into a circular shape at the bottom of the cultivation container to fit the size. After making about 0.5 cm holes at an interval of cm, blanching them at about 90°C for 1 minute, spraying Illite-containing water at intervals of 30 minutes for 1 minute, and then using a thermostat (Hanil, KU-265, Korea) The dried leaves for 5-8 hours at 30-35°C were used as the final pre-treated paulownia leaves when cultivating bean sprouts for secondary cultivation.

(7) Final washing step

This step is the washing, packaging, and product step of the final sprouts grown in (6). When washing the final grown sprouts, the first is washed with tap water, and the second is illite powder (Medex, Y-1000, Korea). : Mixed powder mixed with persimmon juice powder at 1:1 (w/w) is included in 5~10% by weight based on the weight of water, washed 1~2 times in illite and persimmon juice mixture, and finally 2~3 times in tap water After washing the sashimi, the cultivation is completed.

Hereinafter, preferred embodiments of the present invention are as follows.

(1) Raw material The measurement of the yield of bean sprouts according to the amount of illite powder added (wt%) during the first soaking (soaking) is shown in Table 1.

① Yield measurement: The yield of bean sprouts was measured by measuring the weight of bean sprouts per planted rice using an electric indicator scale (SW-1, CAS Co., Korea).

② Results of measuring the yield of bean sprouts according to the amount of illite powder added (wt%) during the first soaking of raw beans

Referring to Table 1, the amount of illite powder added was 0.5% by weight (103.6%), 1% by weight (110.9%), and 5% by weight (106.3%) of all illite powder treatment groups compared to the untreated group (100%) showed a higher yield of 3.6~10.9% than that of untreated

(2) The measurement of the moisture content of bean sprouts according to the amount of illite powder added (wt%) during the first soaking of raw beans is shown in Table 2.

① Moisture content measurement: On the 5th day of growing bean sprouts, 10 g of a random sample was taken, and cotyledon and hypocotyl were immediately divided, and the atmospheric pressure heating and drying method was used in accordance with the AOAC method.

② Measurement result of water content of bean sprouts according to the amount of illite powder added (wt%) during the first soaking of raw beans

Referring to Table 2, cotyledons did not show a significant difference between the untreated group (73.1%) and the illite-treated group (73.9-74.2%), and hypocotylosis untreated group (93.9%) and illite-treated group (93.8~ 94.1%) did not show a significant difference.

(3) The measurement of the mineral content of bean sprouts according to the amount of illite powder added (weight %) during the primary soaking of raw beans is shown in Table 3.

① Measurement of minerals

Na and K were analyzed using an atomic absorption spectrophotometer (Spectra AA800, Varian Co., Australia), and Zn, Mn , Fe , Mg, and Cu were injected into an ICP emission spectrophotometer (38Plus, Jobin Yvon, Co., France). analyzed.

② Measurement result of mineral content of bean sprouts according to the amount of illite powder added (wt%) during the first soaking of raw beans

Referring to Table 3, Cu, K, Mg, and Zn showed lower contents in the illite-treated group than in the untreated, and Fe, Mn, and Na showed higher contents in the illite-treated group than the untreated.

(4) Measurement of the antioxidant effect of bean sprouts according to the pre-treated paulownia leaf treatment during primary cultivation of bean sprouts was shown in Table 4. (5th day of cultivation)

① Measurement of antioxidant effect

(a) Total polyphenol content

The total phenol content was measured using the Folin-Ciocalteu method. On the 5th day of cultivation, 20 ml of methanol was added to 10 g of the bean sprouts sample, followed by centrifugation at room temperature for 24 hours to obtain 5 ml of a supernatant, concentrated under reduced pressure, and then dissolved in 3 ml of methanol and used to measure the polyphenol content. 1 ml of 2% Na ₂ CO ₃ solution was added to 50 μl of the extract in 96 well-plate, and after 3 minutes, 50 μl of 50% Folin-Ciocalteu reagent was added. After 30 minutes, the absorbance value of the reaction solution was measured at 750 nm, and 0.1% gallic acid was used as a standard material. As a measuring device, an ELISA reader (Infinite F50, Tecan, Switzerland) was used.

(b) DPPH radical scavenging ability

DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity was measured by modifying Broyce's method, and the DPPH solution was used at a concentration of 0.1% and measured as follows. After reacting for 30 minutes with DPPH and methanol 1:1 (C), methanol (Co), DPPH and sample 1:1 (S), and sample and methanol 1:1 (So) for 30 minutes, ELISA reader (Sunrise basic, Tecan) at 517 nm , Austria) was used to measure the absorbance. After repeating each experiment three times and averaging, the degree of decrease in absorbance for the control was calculated by the following formula.

DPPH radical scavenging activity (%)=[1-(S-So)/(C-Co)]x100

② Result of antioxidant effect of bean sprouts following pre-treated paulownia leaf treatment during primary cultivation of bean sprouts

Referring to Table 4, the antioxidant effect was 88.99% in the untreated group and 90.33~98.12 in the pre-treated paulownia leaf treatment group. %, and the total polyphenol content was 15.38㎍GAE/g in the untreated group and 19.99~29.33㎍GAE/g in the pretreated paulownia leaf treatment group, showing a higher value in the pre-treated paulownia leaf treatment group than in the untreated group.

(5) The color measurement of bean sprouts according to the mixing ratio of illite powder and persimmon powder when washing cultivated bean sprouts is shown in Table 5.

① Color measurement: Freeze-dry the prepared bean sprouts, spread 5 g of bean sprouts powder evenly on a petri dish, and put plastic wrap on top of it. b (yellowness) value was measured.

② Color results of bean sprouts according to the mixing ratio of illite powder and pre-treated persimmon juice powder when washing cultivated bean sprouts

Referring to Table 5, when washing the cultivated bean sprouts, the result of treating the mixture ratio of illite powder and persimmon juice powder at 1:3,1:2,1:1,2:1,3:1 (w/w) L The (lightness) value was 64.99 ~ 66.23, the a (redness) value was -1.66 ~ -1.34, and the b (yellowness: yellowness) value was 18.99 ~ 20.11, indicating no significant difference between treatments.

Claims (5)

In the bean sprouts cultivation method comprising a washing step, a first soaking step, a first cultivation step, a second soaking step, a second cultivation step, and a final washing step,
In the washing step, 1 kg of beans are washed in tap water, then drained beans are washed 3 times with water mixed with 10 liters of bedrock water and 5 wt% of illite powder washing step,
The first immersion step is a step of immersion in 3 liters of Illite immersion water and immersion at 23±℃ for 5 to 8 hours,
The first cultivation step is a step of culturing for 1 to 2 days at a cultivation temperature of 22 ± ° C, with a watering time of 9 times a day for 2 minutes, and the number of watering 9 times a day using the cultivated water of Illite,
In the second immersion step, the cultivated primary cultivated bean sprouts are mixed in a chimchi container in the shade in a ratio of 1 kg of primary cultivated bean sprouts: Illite immersion water in a ratio of 2: 1 (w/w) and immersed at 20-25° C. for 2 hours. is to do,
In the secondary cultivation step, 1 to 4 pretreated paulownia leaves are laid on the bottom of the cultivation container, the bean sprouts are poured on top, and the black cloth is covered. Cultivated at ±℃ for 4-5 days,
After washing the pre-treated paulownia leaves in tap water, drain the water, cut the bottom of the cultivation container into a circular shape to fit the size, and then make about 0.5 cm holes at about 2 cm intervals and blanch them at about 90 ° C for 1 minute. A method of growing illite bean sprouts, characterized in that after spraying water containing 5% by weight illite at 30-minute intervals for 1 minute, drying at 30-35° C. for 5-8 hours.
The method of claim 1,
In the final washing step, the bean sprouts are first washed with tap water, and then the mixed powder obtained by mixing Illite powder: persimmon juice powder in a 1:1 (w/w) ratio is mixed with water, but the mixed powder is mixed with water 5 A method of growing bean sprouts using illite, characterized in that washing 1 to 2 times in a mixture of illite and persimmon juice containing ~10% by weight, and then washing again in tap water.
The method of claim 1,
The illite-containing water is mixed with 0.5 to 5% by weight of illite powder in 3 liters of mineral water, and then mixed at a speed of 200 to 300 revolutions per minute using a stirrer for 1 hour at 60 to 70° C., the second time A method of growing bean sprouts using Illite, characterized in that it is mixed at 40~45℃ for 2 hours and at 20~25℃ for 2 hours.
delete Bean sprouts, characterized in that consisting of the cultivation method of any one of claims 1 to 3.

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