KR102395741B1 - Preparing method for outline injection composition - Google Patents

Abstract

The present application relates to a method for manufacturing an outline injection composition. The present application includes the steps of: forming a first mixture; forming a second mixture; forming a basic outline injection composition stock solution; and forming an outline injection composition. According to the present invention, fat can be effectively decomposed.

Description

Method for preparing contour injection composition {PREPARING METHOD FOR OUTLINE INJECTION COMPOSITION}

The present application relates to a method for preparing a contour injection composition and a method for breaking down fat using the contour injection composition prepared by the preparation method.

The skin is composed of several different layers, and at the bottom there is a subcutaneous layer for maintaining body temperature and protecting the inside of the body. The subcutaneous layer is known to conserve fat for energy conservation.

However, since the subcutaneous layer preserves fat, even when exercising to reduce weight, fat loss is very slow through recent diets and exercise or cellulite is formed under the skin, making it difficult to lose fat. That is, it is necessary to decompose subcutaneous fat in order to reduce weight, and research on so-called contour injection compositions that decompose such subcutaneous fat by injection or the like are being conducted.

Korean Patent Publication No. 10-2172439, which is the background technology of the present application, relates to a composition for dissolving fat and an injection composition comprising the same.

The present application is to solve the problems of the prior art described above, and an object of the present application is to provide a method for preparing a contour injection composition.

Another object of the present application is to provide a method for decomposing fat through the contour injection composition.

However, the technical problems to be achieved by the embodiments of the present application are not limited to the technical problems described above, and other technical problems may exist.

As a technical means for achieving the above technical problem, the first aspect of the present application is a method for producing a contour injection composition, by adding and mixing animal lipase, carnitine, and deoxycholic acid in a basic solution to a first mixture Forming a, adding and mixing nanoparticles containing Mg(OH) ₂ and hyaluronic acid on the first mixture to form a second mixture, adding sodium chloride and plant extract on the second mixture, A method for preparing a contour injection composition comprising the steps of mixing to form a basic contour injection composition stock solution, and adjusting the pH of the basic contour injection composition stock solution to 7.0 to 8.0 to form a contour injection composition.

According to one embodiment of the present application, the nanoparticles, forming spherical hyaluronic acid particles, and coating Mg(OH) ₂ on the surface of the spherical hyaluronic acid particles It may be produced by a process comprising , but is not limited thereto.

According to one embodiment of the present application, the diameter of the spherical hyaluronic acid particles may be 100 nm to 300 nm, but is not limited thereto.

According to one embodiment of the present application, 10% to 20% of the surface area of the spherical hyaluronic acid particles may be coated with the Mg(OH) ₂ , but is not limited thereto.

According to one embodiment of the present application, the nanoparticles may decompose subcutaneous fat by vibrating by microwaves, but is not limited thereto.

According to one embodiment of the present application, the step of forming the first mixture, the step of forming the second mixture, and the step of forming the basic contour injection composition stock solution each independently increase the temperature by 1° C. per minute. The final temperature may be carried out under the conditions of 30 °C to 50 °C, but is not limited thereto.

According to one embodiment of the present application, the plant extract may include a ginger extract and a phosphorus extract, but is not limited thereto.

In addition, the second aspect of the present application includes the steps of injecting the contour injection composition prepared by the method according to the first aspect into the subcutaneous fat layer, and irradiating microwaves to the skin above the subcutaneous fat layer, and blowing wind into the skin A method for decomposing subcutaneous fat, comprising the step of maintaining the temperature of the skin at 20 ° C to 25 ° C. will be.

According to one embodiment of the present application, the frequency of the microwave may be 100 MHz to 150 MHz, but is not limited thereto.

The above-described problem solving means are merely exemplary, and should not be construed as limiting the present application. In addition to the exemplary embodiments described above, additional embodiments may exist in the drawings and detailed description.

According to the above-described means for solving the problems of the present application, the method for producing the contour injection composition according to the present application can more effectively decompose fat compared to the conventional contour injection composition.

Specifically, the conventional contour injection composition simply contains a component that decomposes subcutaneous fat, and the decomposed fat can be discharged from the subcutaneous fat layer together with body wastes by lymphatic circulation.

However, the contour injection composition prepared by the method according to the present application includes not only the lipolytic component, but also nanoparticles harmless to the human body. The nanoparticles can improve lipolysis efficiency by locally heating the subcutaneous fat layer by irradiated microwaves and at the same time vibrating by microwaves to improve the fat decomposition rate.

In addition, the method for preparing the contour injection composition according to the present application provides a lipolytic material that can be applied to not only facial skin but also parts of the body having a lot of subcutaneous fat such as the abdomen, as well as animals with high obesity.

In addition, the method for preparing the contour injection composition according to the present application is a method for preparing a contour injection composition whose pH is adjusted to have weak acidity, and skin trouble problems such as dry skin can be eliminated or minimized.

However, the effects obtainable herein are not limited to the above-described effects, and other effects may exist.

1 is a schematic diagram showing a method for preparing a contour injection composition according to an embodiment of the present application.

Hereinafter, embodiments of the present application will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art to which the present application pertains can easily carry out.

However, the present application may be implemented in several different forms and is not limited to the embodiments described herein. And in order to clearly explain the present application in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout this specification, when a part is "connected" with another part, this includes not only the case where it is "directly connected" but also the case where it is "electrically connected" with another element interposed therebetween. do.

Throughout this specification, when a member is positioned “on”, “on”, “on”, “on”, “under”, “under”, or “under” another member, this means that a member is positioned on the other member. It includes not only the case where they are in contact, but also the case where another member exists between two members.

Throughout this specification, when a part "includes" a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

As used herein, the terms "about", "substantially" and the like are used in a sense at or close to the numerical value when the manufacturing and material tolerances inherent in the stated meaning are presented, and to aid understanding of the present application. It is used to prevent an unconscionable infringer from using the mentioned disclosure in an unreasonable way. Also, throughout this specification, "step to" or "step to" does not mean "step for".

Throughout this specification, the term "combination of these" included in the expression of the Markush form means one or more mixtures or combinations selected from the group consisting of the components described in the expression of the Markush form, and the components It is meant to include one or more selected from the group consisting of.

Throughout this specification, reference to “A and/or B” means “A or B, or A and B”.

Hereinafter, the method for preparing the contour injection composition of the present application will be described in detail with reference to embodiments and examples and drawings. However, the present application is not limited to these embodiments and examples and drawings.

As a technical means for achieving the above technical problem, the first aspect of the present application is a method for producing a contour injection composition, by adding and mixing animal lipase, carnitine, and deoxycholic acid in a basic solution to a first mixture Forming a, adding and mixing nanoparticles containing Mg(OH) ₂ and hyaluronic acid on the first mixture to form a second mixture, adding sodium chloride and plant extract on the second mixture, A method for preparing a contour injection composition comprising the steps of mixing to form a basic contour injection composition stock solution, and adjusting the pH of the basic contour injection composition stock solution to 7.0 to 8.0 to form a contour injection composition.

The contour injection composition according to the present application is a lipolytic injection, and refers to a composition that breaks down fat cells inside the skin. Since the decomposed fat cells are naturally discharged through blood vessels through physiological action, they are safer than liposuction and are in the spotlight for obesity treatment. are receiving

First, animal lipase, carnitine, and deoxycholic acid are added to a basic solution and mixed to form a first mixture.

According to one embodiment of the present application, the pH of the basic solution may be 9 to 10, but is not limited thereto.

Carnitine according to the present application can promote the energy conversion of fatty acids by introducing fatty acids in the body into mitochondria and decomposing fatty acids. In addition, deoxycholic acid according to the present application is a substance that helps in digestion and absorption of fat, and has an effect of destroying subcutaneous fat cells when directly injected into subcutaneous adipose tissue. In addition, the animal lipase is an enzyme that breaks down fat, and all of them are effective substances for fat breakdown.

At this time, the animal lipase may be activated in a weak basic atmosphere having a pH of 7.0 to 9.0, and is not activated but not decomposed in a strong basic atmosphere (pH greater than 9.0). Accordingly, when the pH of the basic solution is a very strong base, for example, in an environment having a pH of 8.0 to 10.0, the animal lipase may exist in an inactivated state without being decomposed in the basic solution.

In general, since enzymes are also sensitive to heat, it is necessary to control the temperature so as not to become excessively high in the process of forming the contour injection composition.

According to one embodiment of the present application, the step of forming the first mixture may be performed under conditions in which the temperature is increased by 1°C per minute and the final temperature is 30°C to 50°C, but is not limited thereto. For example, the first mixture is formed in an environment where the final temperature is 35°C while stirring while raising the temperature by 1°C per minute in the process of adding and mixing animal lipase, carnitine, and deoxycholic acid in a basic solution can be

Specifically, the first mixture is stirred in an environment in which the initial temperature is 20°C, the temperature is increased by 1°C per minute and stirred for 15 minutes, and when the final temperature reaches 35°C, the temperature rise is stopped and the temperature is maintained for 1 hour can be formed.

Then, the nanoparticles containing Mg(OH) ₂ and hyaluronic acid are added to the first mixture and mixed to form a second mixture.

According to one embodiment of the present application, the nanoparticles, forming spherical hyaluronic acid particles, and coating Mg(OH) ₂ on the surface of the spherical hyaluronic acid particles It may be produced by a process comprising , but is not limited thereto.

Hyaluronic acid according to the present application, as a kind of biosynthetic natural material, does not cause serious side effects to the human body and has a characteristic of causing only mild side effects to the extent of slight inflammation. In addition, Mg(OH) ₂ according to the present application is an anti-inflammatory agent, which effectively suppresses inflammation that occurs when the hyaluronic acid is decomposed, and can help the spherical hyaluronic acid particles vibrate.

According to one embodiment of the present application, the nanoparticles may decompose subcutaneous fat by vibrating by microwaves, but is not limited thereto.

Specifically, when the nanoparticles are irradiated with microwaves, the spherical hyaluronic acid particles or Mg(OH) ₂ formed on the surface of the particles may vibrate and generate heat. At this time, the vibration of the spherical hyaluronic acid particles or Mg(OH) ₂ helps to physically crush the fat cells in the subcutaneous fat layer, and the heat can supply an energy source necessary for the physical or chemical decomposition process of the subcutaneous fat layer. .

According to one embodiment of the present application, the diameter of the spherical hyaluronic acid particles may be 100 nm to 300 nm, but is not limited thereto.

According to one embodiment of the present application, 10% to 20% of the surface area of the spherical hyaluronic acid particles may be coated with the Mg(OH) ₂ , but is not limited thereto.

According to one embodiment of the present application, the method for forming the nanoparticles includes the steps of mixing hyaluronic acid and a hydrophobic material and self-assembling in distilled water to form spherical hyaluronic acid particles, Mg in a solution containing the spherical hyaluronic acid particles (OH) ₂ It may include the step of coating the surface of the spherical hyaluronic acid particles with Mg(OH) ₂ by heating and stirring, but is not limited thereto. At this time, the process of coating the surface of the spherical hyaluronic acid particles with Mg(OH) ₂ may be performed at 40° C. to 50° C., and Mg(OH) ₂ may be added when mixing hyaluronic acid and a hydrophobic material. there is.

Specifically, in the process of forming nanoparticles from aluronic acid, 600 mg of hyaluronic acid is dissolved in 120 ml of formamide, and 30 mg of Mg(OH) ₂ is dissolved in 200 ml of dimethyl formamide, and then the formamido -It may include the process of stirring at room temperature for 24 hours while slowly dropping it into the hyaluronic acid solution. In this case, the ratio between the mass of Mg(OH) ₂ and the mass of hyaluronic acid may be 0.01: 1 to 0.1: 1, but is not limited thereto.

If the ratio between the mass of Mg(OH) ₂ and the mass of hyaluronic acid is less than 0.01: 1, an area corresponding to less than 10% of the surface area of the spherical hyaluronic acid particles may be coated with Mg(OH) ₂ , If it is greater than 0.1: 1, it was confirmed that an area exceeding 20% of the surface area of the spherical hyaluronic acid particles may be coated with Mg(OH) ₂ .

As will be described later, the nanoparticles added to the first mixture are added in a dispersed form in an aqueous solution.

Mg(OH) ₂ is to suppress inflammation that occurs during the decomposition of hyaluronic acid, and may exist in the form of Mg ²⁺ ions in the blood. When there is too much magnesium ion or magnesium in the body, there are problems that can cause problems such as low blood pressure, depression, fatigue, dizziness, salt addiction, poor concentration, diarrhea, muscle weakness, hypotension, respiratory depression, and cardiac arrest.

In general, as magnesium is ingested into the body through food, Mg(OH) ₂ coated on the surface area of the spherical hyaluronic acid particles needs to be minimized to the extent that inflammation can be suppressed.

For example, when Mg(OH) ₂ is coated with less than 10% of the surface area of the spherical hyaluronic acid particles, there is a problem in that inflammation that may occur in the decomposition process of the spherical hyaluronic acid particles cannot be suppressed. On the other hand, when Mg(OH) ₂ is coated with more than 20% of the surface area of the spherical hyaluronic acid particles, the concentration of Mg partially increases at the site where the contour injection composition is inoculated, and side effects such as dizziness or diarrhea may occur. there is.

According to the exemplary embodiment of the present application, the forming of the second mixture may be performed under conditions in which the temperature is increased by 1°C per minute and the final temperature is 30°C to 50°C, but is not limited thereto. For example, the second mixture may be formed in an environment where the final temperature is 40°C while stirring the process of adding and mixing nanoparticles onto the first mixture while increasing the temperature by 1°C per minute.

Specifically, the second mixture is formed in an environment in which the initial temperature is 20°C, the temperature is increased by 1°C per minute, stirred for 20 minutes, and when the final temperature reaches 40°C, the temperature rise is stopped and the temperature is maintained for 1 hour can be

In this regard, before performing the step of forming the second mixture, the nanoparticles may be dispersed in distilled water, but is not limited thereto.

The process of dispersing the nanoparticles in distilled water includes adding the nanoparticles to distilled water, irradiating the distilled water with microwaves, and stirring while increasing the temperature of the distilled water.

The nanoparticles may be mixed by Mg(OH) ₂ during the manufacturing process, but some non-decomposed hyaluronic acid crosslinkers may exist in the form of a suspension. When these nanoparticles are added to the first mixture and mechanically mixed, the nanoparticles may not be evenly dispersed on the first mixture by the non-decomposed hyaluronic acid crosslinker. In general, when the temperature is raised and stirred, the dispersion of the particles in the solution can proceed smoothly, but the first mixture contains an animal lipase enzyme, and if the temperature is too high, the animal lipase may be destroyed, and Mg ( There is a possibility that the spherical hyaluronic acid particles not coated with OH) ₂ are bound to each other.

In order to prevent this problem, the nanoparticles injected into the first mixture are formed by coating Mg(OH) ₂ on the surface of the spherical hyaluronic acid particles, and then added to distilled water to disperse through stirring and then the first It can be mixed with the mixture.

Then, sodium chloride and plant extract are added to the second mixture and mixed to form a basic contour injection composition stock solution.

Sodium chloride according to the present application can destroy fat cells in a way that induces osmotic pressure by generating a concentration difference between the portion into which the contour injection composition is injected and the fat cells.

According to one embodiment of the present application, the plant extract may include a ginger extract and a phosphorus extract, but is not limited thereto.

The ginger extract may include gingerol and shogaol. The gingerol and shogaol are known to be effective substances for fat decomposition.

In addition, as the injin extract has an anti-inflammatory and analgesic action, it can suppress pain that occurs when the contour injection composition is injected into the subcutaneous fat layer or in the process of lipolysis.

According to one embodiment of the present application, the step of forming the basic contour injection composition stock solution may be performed under conditions where the temperature is increased by 1 ℃ per minute and the final temperature is 30 ℃ to 50 ℃, but is not limited thereto.

Specifically, the basic contour injection composition stock solution has an initial temperature of 20 ° C., and the temperature is increased by 1 ° C. per minute and stirred for 20 minutes. When the final temperature reaches 30 ° C., the temperature rises and the temperature is maintained for 1 hour. can be formed in

Then, the pH of the basic contour injection composition stock solution is adjusted to 7.0 to 8.0 to form a contour injection composition. Specifically, the contour injection composition may be formed by adjusting the pH of the basic contour injection composition stock solution to 7.5.

As described above, the animal lipase can be activated in a weakly acidic atmosphere having a pH of 7.0 to 9.0, and the general skin pH has a weakly acidic atmosphere of about 5.5 to 5.9. In addition, considering that the pH of the basic solution is 9 to 10, if the pH of the basic contour injection composition is directly injected into the skin without adjusting the pH, the pH balance on the skin surface and inside is broken, making the skin dry, etc. Side effects may occur.

However, the contour injection composition according to the present application is diluted by injecting an acidic substance such as ascorbic acid to adjust the pH after being prepared in a basic solution, unlike when the diluted basic contour injection composition stock solution is injected into the skin It is possible to supply insufficient nutrients while having no or minimal side effects.

The step of adjusting the pH of the basic contour injection composition stock solution may be performed by injecting an acidic substance into the basic contour injection composition stock solution, and adding distilled water to control the concentration. At this time, the type and amount of the acidic substance may be adjusted so that the pH of the contour injection composition is 7.5 after the acidic substance is injected in consideration of the pH, and distilled water is added to adjust the concentration of the substance.

In this regard, the contour injection composition having a pH of 7.0 to 8.0 may be formed by adjusting the pH of the basic contour injection composition stock solution and then diluting the concentration with distilled water.

In addition, the second aspect of the present application includes the steps of injecting the contour injection composition prepared by the method according to the first aspect into the subcutaneous fat layer, and irradiating microwaves to the skin above the subcutaneous fat layer, and blowing wind into the skin A method for decomposing subcutaneous fat, comprising the step of maintaining the temperature of the skin at 20 ° C to 25 ° C. will be.

With respect to the method for decomposing subcutaneous fat according to the second aspect of the present application, detailed descriptions of parts overlapping with the first aspect of the present application are omitted, but even if the description is omitted, the contents described in the first aspect of the present application The same can be applied to both sides.

First, the contour injection composition prepared by the method according to the first aspect is injected into the subcutaneous fat layer.

Then, microwaves are irradiated to the skin above the subcutaneous fat layer.

Then, air is applied to the skin to maintain the temperature of the skin at 20°C to 25°C.

As described above, the contour injection composition contains not only subcutaneous fat decomposing components such as animal lipase, carnitine, deoxycholic acid, sodium chloride, and plant extracts for decomposing subcutaneous fat, but also nano for decomposing subcutaneous fat through microwaves, etc. particles may be included.

The nanoparticles include spherical hyaluronic acid particles and Mg(OH) ₂ coated on the surface of the spherical hyaluronic acid particles. When the nanoparticles are irradiated with microwaves, the nanoparticles vibrate so that vibration is applied to the subcutaneous fat of the subcutaneous fat layer, Subcutaneous fat may be decomposed by the vibration. The decomposed fat may be mixed with the fat decomposed by the subcutaneous fat decomposition component and discharged to the outside of the body.

In this regard, the step of irradiating the microwave to the skin and the step of maintaining the temperature of the skin may be performed at the same time, but is not limited thereto.

At this time, when the microwave is irradiated, heat may be generated in the skin due to the vibration of moisture in the skin, and low-temperature burns or first-degree burns may occur in the skin above the subcutaneous fat layer by the heat. In order to prevent this problem, the skin can be blown with air or a cooling material (such as low-temperature water) can be brought into contact with the skin. It can vibrate and generate additional heat.

According to one embodiment of the present application, the frequency of the microwave may be 100 MHz to 150 MHz, but is not limited thereto. Preferably, the frequency of the microwave may be 114 MHz.

When the frequency of the microwave is less than 100 MHz, the degree of vibration of the nanoparticles is low, so that there may be no effect of subcutaneous fat decomposition by the nanoparticles. In addition, when the frequency of the microwave is more than 150 MHz, the vibration by the nanoparticles is active and the effect of subcutaneous fat decomposition is excellent, but there is a risk of burns to the skin due to the generation of a lot of heat in the skin.

The method for decomposing subcutaneous fat can be applied to not only facial skin but also parts of the body having a lot of subcutaneous fat, such as the abdomen, as well as animals with a high degree of obesity.

The method of decomposing the subcutaneous fat can be analyzed by comparing the pictures before and after the operation. Specifically, when the contour injection composition is injected into the cheeks for the decomposition of the subcutaneous fat, the degree of subcutaneous fat decomposition can be confirmed by measuring the distance from the cheeks based on the position of the two eyes.

In this regard, the amount of injecting the contour injection composition into the skin for subcutaneous lipolysis may be up to 10 ml at a time.

The present invention will be described in more detail through the following examples, but the following examples are for illustrative purposes only and are not intended to limit the scope of the present application.

[Example 1-1]

First, 1 μg of animal lipase, 10 g of carnitine, and 10 g of deoxycholic acid are added to 1 L of a basic solution having a pH of 9.7, the initial temperature is 20°C, and the temperature is increased by 1°C per minute for 15 minutes. While stirring, when the final temperature reached 35°C, the temperature was stopped and stirred in an environment where the temperature was maintained at 35°C for 1 hour, followed by cooling (first mixture).

In addition, 600 mg of hyaluronic acid was dissolved in 120 ml of formamide, 30 mg of Mg(OH) ₂ was dissolved in 200 ml of dimethyl formamide, and then slowly dropped into the formamido-hyaluronic acid solution at room temperature 24 By performing a stirring process for a period of time, a part of the surface was formed of nanoparticles containing spherical hyaluronic acid particles coated with Mg(OH) ₂ . At this time, the ratio between the mass of Mg(OH) ₂ and the mass of hyaluronic acid is 0.05:1.

Then, the aqueous solution was mixed with the first mixture, stirred, and then cooled. At this time, the stirring condition is an environment in which the initial temperature is 20°C, the temperature is increased by 1°C per minute, stirred for 20 minutes, and when the final temperature reaches 40°C, the temperature rise is stopped and the temperature is maintained at 40°C for 1 hour can (second mixture).

Then, 100 mg of sodium chloride, 5 g of ginger extract and 5 g of phosphorus extract were added to the second mixture and mixed. At this time, the mixing conditions may be an environment in which the initial temperature is 20°C, the temperature is raised by 1°C per minute, stirred for 20 minutes, and when the final temperature reaches 30°C, the temperature rise is stopped and the temperature is maintained at 30°C for 1 hour There is (basic contour injection composition stock solution).

Then, 10 mg of ascorbic acid was added to the stock solution of the basic contour injection composition, and distilled water was added to form a contour injection composition whose pH was adjusted to about 7.5.

[Example 1-2]

Then, the formed contour injection composition was injected into the facial skin, and while the facial skin was irradiated with a microwave of 100 MHz, air was blown so that the body temperature of the facial skin was maintained at 25°C. This experiment was performed on 10 people.

[Comparative Example 1-1]

The same process as that of Example 1-1 was performed, but the step of adding animal lipase was omitted.

[Comparative Example 1-2]

The same process as in Example 1-1 was performed, but the step of adding carnitine was omitted.

[Comparative Example 1-3]

The same process as in Example 1-1 was performed, but the step of adding deoxycholic acid was omitted.

[Comparative Example 1-4]

The same process as that of Example 1-1 was performed, but the step of adding nanoparticles was omitted.

[Comparative Example 1-5]

The same process as in Example 1-1 was performed, but the step of adding sodium chloride was omitted.

[Comparative Example 1-6]

The same process as in Example 1-1 was performed, but the step of adding the ginger extract and the phosphorus extract was omitted.

[Comparative Example 2-1]

The same procedure as in Example 1-1 was performed, except that in the process of preparing the first mixture, the second mixture, and the basic contour injection composition stock solution, the temperature was fixed at the initial temperature (20° C.) and for 80 minutes without increasing the temperature. stirred.

[Comparative Example 2-2]

The same procedure as in Example 1-1 was performed, except that the final temperature (35° C., 40° C., and 30 ℃) and stirred for 80 minutes.

[Comparative Example 2-3]

The same procedure as in Example 1-1 was performed, except that the temperature conditions were changed in the process of preparing the first mixture, the second mixture, and the basic contour injection composition stock solution. Specifically, the changed temperature condition is that the initial temperature is 20°C, the temperature is increased by 5°C per minute and stirred (3 minutes, 4 minutes, 2 minutes, respectively), and the final temperature (35°C, 40°C, and 30°C, respectively) ) and stirred for 77 min, 76 min, and 78 min, respectively.

[Comparative Example 3-1]

Nanoparticles were prepared using the same procedure as in Example 1-1, but using 3 mg of Mg(OH) ₂ .

[Comparative Example 3-2]

Nanoparticles were prepared in the same manner as in Example 1-1, but using 300 mg of Mg(OH) ₂ .

[Comparative Example 4-1]

The same procedure as in Example 1-1 was performed, but the step of adding an acidic substance to the prepared basic contour injection composition stock solution was omitted to form a basic contour injection composition in which the basic contour injection composition stock solution was diluted.

[Comparative Example 4-2]

The same procedure as in Example 1-1 was performed, but the step of adding an acidic substance to the prepared basic contour injection composition stock solution and the dilution step were omitted.

[Comparative Example 5-1]

The same process as in Example 1-2 was performed, but the step of irradiating microwaves was omitted.

[Comparative Example 5-2]

The same process as that of Example 1-2 was performed, but the step of providing air was omitted.

[Comparative Example 5-3]

The same process as in Example 1-2 was performed, but both the microwave irradiation step and the air blowing step were omitted.

[Experimental Example 1]

The process of Example 1-2 was performed using the contour injection composition prepared through the processes of Example 1-1 and Comparative Examples 1-1 to 1-6. At this time, the inoculation method of the contour injection composition follows the method of Example 1-2.

Example 1-1 Comparative Example 1-1 Comparative Example 1-2 Comparative Example 1-3 Comparative Example 1-4 Comparative Example 1-5 Comparative Example 1-6 immediately after the procedure
degree of pain experienced 1.5 1.6 1.4 1.6 1.4 1.5 1.7 1 week after surgery
degree of pain experienced 0.2 0.1 0.3 0.4 0.2 0.3 1.0 left before surgery
(mm) 53.1 52.3 51.2 54.6 53.2 52.9 56.4 right side before surgery
(mm) 52.6 51.8 52.8 53.2 52 52.7 55.8 left after surgery
(mm) 47.2 47.2 46.4 50.3 51.2 49.3 53.2 right side after surgery
(mm) 47.5 47.1 48.2 49.2 49.9 49.2 52.9 left difference
(mm) 5.9 5.1 4.8 4.3 2 3.6 3.2 right difference
(mm) 5.1 4.7 4.6 4 2.1 3.5 2.9

In Table 1, the degree of pain experienced is a score of inflammation at the inoculation site, and 0 points for no pain, 1 point for mild pain, 2 points for moderate pain, and 3 points for severe pain. , and left means the horizontal distance from the center of the left eye to the left cheek, and right means the horizontal distance from the center of the right eye to the right cheek. In this regard, the pain level experienced, the left side, and the right side may be equally applied to Tables 2 to 4 to be described later.

Referring to Table 1, the contour injection composition according to Example 1-1 includes all of animal lipase, carnitine, deoxycholic acid, nanoparticles, sodium chloride, ginger extract, and phosphorus extract, of which Compared to the contour injection composition (Comparative Examples 1-1 to 1-6) in which any one is excluded, the facial skin fat removal effect may be excellent. In particular, Comparative Examples 1-4 and Comparative Examples 1-5 do not contain nanoparticles or sodium chloride, respectively, so the fat removal effect may be insufficient compared to other compositions, and Comparative Examples 1-6 contains both the ginger extract and the phosphorus extract. If you do not do this, the fat removal effect is insufficient, and a very slight inflammation may remain after the procedure.

[Experimental Example 2]

The physical properties of the contour injection compositions prepared through the processes of Example 1-1 and Comparative Examples 2-1 to 2-3 were compared with the fat decomposition effect. At this time, the inoculation method of the contour injection composition follows the method of Example 1-2.

Example 1-1 Comparative Example 2-1 Comparative Example 2-2 Comparative Example 2-3 immediately after the procedure
degree of pain experienced 1.5 1.7 1.4 1.8 1 week after surgery
degree of pain experienced 0.1 0.6 0.9 1.2 left before surgery
(mm) 53.1 52.9 53.4 54.7 right side before surgery
(mm) 52.6 52.1 53.1 54.3 left after surgery
(mm) 47.2 49 51.2 52.1 right side after surgery
(mm) 47.5 48.4 51.4 52 left difference
(mm) 5.9 3.9 2.2 2.6 right difference
(mm) 5.1 3.7 1.7 2.3

Referring to Table 2, it can be confirmed that the composition stirred at a low temperature (Comparative Example 2-1) has somewhat insufficient fat decomposition effect and has a cloudy feeling compared to the composition of Example 1-1 whether the stirring is performed smoothly. there was. In addition, the composition of Comparative Example 2-2 was formed at a relatively high temperature from the beginning, unlike the material of Example 1-1, and the composition of Comparative Example 2-3 had a temperature increase rate unlike the composition of Example 1-1. formed under rapid conditions. As a result, it was confirmed that the compositions of Comparative Examples 2-2 and 2-3 had little lipolytic effect, and in particular, the composition of Comparative Example 2-3 showed severe inflammation at the inoculation site compared to other compositions. [Experimental Example 3]

The lipolytic effect of the contour injection composition prepared through the process of Example 1-1, Comparative Example 3-1, and Comparative Example 3-2 was compared. At this time, the inoculation method of the contour injection composition follows the method of Example 1-2.

Example 1-1 Comparative Example 3-1 Comparative Example 3-2 immediately after the procedure
degree of pain experienced 1.5 1.4 1.3 1 week after surgery
degree of pain experienced 0.1 0.6 0.7 left before surgery
(mm) 53.1 51.9 55.2 right side before surgery
(mm) 52.6 51.4 54.7 left after surgery
(mm) 47.2 50.7 49.5 right side after surgery
(mm) 47.5 50.1 49.3 left difference
(mm) 5.9 1.2 5.7 right difference
(mm) 5.1 1.3 5.4

Referring to, the composition prepared by using a small amount of Mg(OH) ₂ (Comparative Example 3-1) has a problem in that the effect of removing subcutaneous fat is small. However, the composition prepared by using a large amount of Mg(OH) ₂ (Comparative Example 3-2) has a lipolytic effect similar to that of Example 1-1, but a slight dizziness, decreased concentration, drowsiness, fatigue, It was reported that symptoms such as diarrhea continued to occur for 1 week after inoculation.

[Experimental Example 4]

The progress after inoculation of the contour injection composition prepared through the process of Example 1-1, Comparative Example 4-1, and Comparative Example 4-2 was observed.

Since the compositions of Comparative Examples 4-1 and 4-2 are strong basic substances, unlike the inoculation site of the composition of Example 1-1, the composition of Comparative Example 4 has a slight fever or slippery skin at the inoculation site. There were other problems. In addition, after the fever subsided, the inoculation site became rough for about 2 weeks, acne, some urticaria, and erythema occurred.

[Experimental Example 5]

The composition of Example 1-1 was inoculated according to the method of Example 1-2 and Comparative Examples 5-1 to 5-3.

Example 1-1 Comparative Example 5-1 Comparative Example 5-2 Comparative Example 5-3 immediately after the procedure
degree of pain experienced 1.5 1.6 1.4 1.3 1 week after surgery
degree of pain experienced 0.1 0.1 0.2 0.2 left before surgery
(mm) 53.1 53.2 52.9 54.2 right side before surgery
(mm) 52.6 52.9 52.3 53.8 left after surgery
(mm) 47.2 49.1 47.5 50.4 right side after surgery
(mm) 47.5 49.2 47.4 50.6 left difference
(mm) 5.9 4.1 5.4 3.8 right difference
(mm) 5.1 3.7 4.9 3.2

Referring to Table 4, in the case of the inoculation method without air (Comparative Example 5-2), the lipolysis effect was excellent compared to Comparative Examples 5-1 and 5-3, but the inoculator reported that the inoculation part had severe fever. did In addition, in Comparative Examples 5-1 and 5-3, the fat was decomposed by substances other than nanoparticles (deoxycholic acid, carnitine, etc.) because microwaves were not irradiated, so that the lipolytic effect was lower than that of Example 1-1. low can be seen.

Claims (9)

A method for preparing a contour injection composition comprising:
Forming a first mixture by adding and mixing animal lipase, carnitine, and deoxycholic acid on a basic solution;
Forming a second mixture by adding and mixing nanoparticles containing Mg(OH) ₂ and hyaluronic acid on the first mixture;
Forming a basic contour injection composition stock solution by adding and mixing sodium chloride and a plant extract on the second mixture; and
adjusting the pH of the basic contour injection composition stock solution to 7.0 to 8.0 to form a contour injection composition;
containing,
A method for preparing a contour injection composition.
The method of claim 1,
The nano-particles, forming spherical hyaluronic acid particles, and Mg(OH) ₂ on the surface of the spherical hyaluronic acid particles will be prepared by a process comprising the step of coating a method for producing a contour injection composition.
3. The method of claim 2,
The spherical hyaluronic acid particles have a diameter of 100 nm to 300 nm, a method for producing a contour injection composition.
3. The method of claim 2,
10% to 20% of the surface area of the spherical hyaluronic acid particles is coated with the Mg(OH) ₂ A method for producing a contour injection composition.
The method of claim 1,
The method for producing a contour injection composition, wherein the nanoparticles are to decompose subcutaneous fat by vibrating by microwaves.
The method of claim 1,
The step of forming the first mixture, the step of forming the second mixture, and the step of forming the basic contour injection composition stock solution each independently increase the temperature by 1° C. per minute so that the final temperature is 30° C. to 50° C. A method for producing a contour injection composition, which is carried out under the conditions of phosphorus.
The method of claim 1,
The plant extract is a method for producing a contour injection composition comprising a ginger extract and a phosphorus extract.
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