KR102374289B1 - Preparing Method for Germinated Brown Rice Having Increased Contents of GABA - Google Patents

Abstract

The present invention relates to a method for producing germinated brown rice with an increased GABA content, and more particularly, GABA (Gamma-aminobutyric acid), which is widely used as a food, additive, and cosmetic material, through control of germination conditions, germination with increased content It relates to a method for producing brown rice.
The method for producing germinated brown rice with increased GABA content according to the present invention is at least one selected from the group consisting of calamansi, aloes tree , cinnamon leaf, Jeju porridge, Korean thistle, peanut husk, chaga mushroom and Arabidopsis. It includes the step of immersing and germinating brown rice in an immersion water containing a plant extract.
According to the present invention, it is possible to significantly increase the content of GABA during germination of brown rice, and when the germinated brown rice extract extracted therefrom is applied to a cosmetic composition, it is possible to prepare a natural cosmetic with excellent whitening and antioxidant effects.

Description

{Preparing Method for Germinated Brown Rice Having Increased Contents of GABA}

The present invention relates to a method for producing germinated brown rice with an increased GABA content, and more particularly, GABA (Gamma-aminobutyric acid), which is widely used as a food, additive, and cosmetic material, through control of germination conditions, germination with increased content It relates to a method for producing brown rice.

GABA (gamma-aminobutyric acid, gamma-aminobutyric acid) was discovered in a mammalian brain extract by Roberts and Awapara in 1950 and identified as an inhibitory transmitter in the crustacean renal receptor. In addition to nerve stabilization, GABA is attracting attention as a functional material with various physiological activities such as blood pressure increase inhibition, anti-stress, brain metabolism promotion, liver function improvement, and growth hormone regulation. is being used In particular, tablet-type products mixed with vitamins are mainly related to the nerve stabilization function, and various types of products, ranging from cosmetics to pharmaceuticals, are being marketed and sold.

On the other hand, germinated brown rice has the characteristics of being easy to cook and easy to eat compared to brown rice.

When brown rice germinates, α-amylase is newly produced in the embryo, and enzymes such as protease and β-amylase are biosynthesized or activated. Activation of amylase, protease, decarboxylase, etc. brings about component changes characteristic of germinated brown rice, and it is known that gamma-aminobutyric acid (GABA) generally increases when brown rice is soaked in water.

However, in order to further increase the GABA content during germination of brown rice, cold shock, mechanical stimulation, heat shock, hypoxia, cytosolic acidification, and water stimulation stress), and there have been many studies using phytohormones.

Korean Patent Registration No. 1336295 discloses, "The first brown rice germination step in which maintaining 3 to 5 hours in dark conditions is repeated twice, and the cycle of maintaining the brown rice sprouts in light conditions and dark conditions for 3 to 5 hours, respectively, twice is repeated twice. The growth step, the secondary growth step of brown rice shoots in which the cycle of maintaining the green rice shoots under light conditions and dark conditions for 2 to 4 hours, respectively, is repeated twice, and maintaining the micros sprouts under light conditions and dark conditions for 1 to 3 hours, respectively, 4 to 10 times Disclosed is "a method of growing brown rice sprouts including repeated tertiary growth steps", and Korean Patent No. 0905772 discloses "a mixture of 0.01-0.25 wt% sodium chloride and 0.01-0.25 wt% chitosan solution in the germination medium as an elicitor. A method for producing germinated brown rice having an increased content of GABA and phenolic compounds, including the step of germinating brown rice in a germination medium comprising

However, these methods have an improved GABA enhancing effect compared to simply immersing brown rice in water, but the improved GABA content is not very satisfactory.

Accordingly, the present inventors have made efforts to solve the above problems, and as a result of germinating brown rice, plant extracts such as calamansi, aloes tree , cinnamon leaf, Jeju porridge, Korean thistle, peanut shell, chaga mushroom, and Arabidopsis as immersion water When oxygen is supplied, it was confirmed that germinated brown rice with significantly increased GABA content can be prepared, and the present invention has been completed.

It is an object of the present invention to provide a method for producing germinated brown rice having an increased GABA content and a method for preparing a germinated brown rice extract having an increased GABA content.

Another object of the present invention is to provide a natural cosmetic composition having excellent moisturizing, antioxidant and whitening effects.

In order to achieve the above object, the present invention is an immersion water containing one or more plant extracts selected from the group consisting of calamansi, aloes tree , sour tree leaves, Jeju porridge, Korean thistle, peanut shells, chaga mushroom and Arabidopsis. It provides a method for producing germinated brown rice having an increased GABA content, comprising the step of immersing and germinating brown rice.

The present invention also provides (a) brown rice in immersion water containing one or more plant extracts selected from the group consisting of calamansi, aloes tree , cinnamon leaf, Jeju porridge, Korean thistle, peanut husk, chaga mushroom, and Arabidopsis. soaking and germinating; and (b) crushing germinated brown rice, then adding purified water and stirring at 30 to 60° C. for 6 to 10 hours to prepare a germinated brown rice extract. A method for preparing an extract is provided.

The present invention also provides a natural cosmetic composition comprising a germinated brown rice extract having an increased GABA content.

According to the present invention, it is possible to significantly increase the content of GABA during germination of brown rice, and when the germinated brown rice extract extracted therefrom is applied to a cosmetic composition, it is possible to prepare a natural cosmetic with excellent whitening and antioxidant effects.

1 is a photograph of germinated brown rice prepared according to an embodiment of the present invention.

In the present invention, it was attempted to confirm that germinated brown rice with a significantly increased GABA content could be produced when the germination conditions were controlled during the germination of brown rice.

In the present invention, brown rice was immersed in an immersion water containing plant extracts predicted to increase the GABA content of brown rice upon germination, and oxygen was supplied. As a result, it was confirmed that there was a difference in the GABA content of germinated brown rice depending on the type of plant extract and the presence or absence of oxygen supply.

That is, in one embodiment of the present invention, brown rice is immersed in immersion water containing 48 plant extracts, respectively, and the germination of brown rice is induced while supplying oxygen, and among the plant extracts , calamansi, aloes, cinnamon leaves, It was confirmed that the GABA content of germinated brown rice was significantly increased when Jeju porridge, Korean thistle, peanut husks, chaga mushroom, and baby evening primrose were included in the immersion water.

Accordingly, in one aspect, the present invention is brown rice in immersion water containing one or more plant extracts selected from the group consisting of calamansi, aloes tree , cinnamon leaf, Jeju rye, Korean thistle, peanut shell, chaga mushroom, and Arabidopsis It relates to a method for producing germinated brown rice having an increased GABA content, comprising the step of immersing and germinating.

The plant extract may be extracted by immersing, boiling, hot water extraction, or supercritical extraction or microwave extraction of a plant dried in an organic solvent such as ethanol or water, and may be obtained through filtration and concentration.

The immersion water may be used at a concentration of 10 to 100% by volume of the immersion water depending on the type of plant extract.

In the present invention, it is characterized in that oxygen is supplied during the soaking time in order to further increase the GABA content of germinated brown rice. The oxygen is not particularly limited, but it is preferable to supply 0.5 to 10 L/min.

In addition, in the present invention, the germination of brown rice can be carried out at 28-34 ° C., preferably at 31-33 ° C., and immersion for 20 to 28 hours until the length of the germinated shoot becomes 1.8 to 2.2 mm. desirable.

If out of the above temperature conditions or the length of the germinated shoot is less than 1.8 mm or exceeds 2.2 mm, the content of GABA may not increase to the maximum.

In another aspect, the present invention is immersed in brown rice in immersion water containing one or more plant extracts selected from the group consisting of calamansi, aloes wood , cinnamon leaf, Jeju porcini, Korean thistle, peanut shell, chaga mushroom, and Arabidopsis. and germinating; and (b) crushing germinated brown rice, then adding purified water and stirring at 30 to 60° C. for 6 to 10 hours to prepare a germinated brown rice extract. It relates to a method for preparing an extract.

Even for germinated brown rice with an increased GABA content, there is a difference in the extracted GABA content depending on the extraction method.

In the present invention, the germinated brown rice extract with increased GABA content can be extracted by immersion in an organic solvent such as ethanol or water, hot water, distillation, or extraction by supercritical extraction or microwave extraction, and the filtration and concentration process Although it can be obtained through germinated brown rice, it is preferable to crush germinated brown rice in order to extract maximum GABA from germinated brown rice, then add purified water and stir at 30 to 60° C. for 6 to 10 hours.

The present invention also relates to a natural cosmetic composition comprising a germinated brown rice extract having an increased GABA content prepared by the method for preparing a germinated brown rice extract having an increased GABA content.

The germinated brown rice extract having an increased GABA content is characterized in that it contains 0.5 to 50 parts by weight based on 100 parts by weight of the natural cosmetic composition. When the amount of the germinated brown rice extract with an increased GABA content is less than 0.5 parts by weight based on the total weight of the natural cosmetic composition, antioxidant, anti-wrinkle and whitening effects cannot be sufficiently obtained, so the function as a skin cosmetic is poor, and 50 parts by weight When it is exceeded, it is uneconomical because it can exhibit sufficient efficacy even if it does not contain more.

The natural cosmetic composition of the present invention is an external skin ointment, cream, softening lotion, nourishing lotion, pack, essence, hair tonic, shampoo, conditioner, hair conditioner, hair treatment, gel, skin lotion, skin softener, skin toner, astringent, Lotion, Milk Lotion, Moisture Lotion, Nourishing Lotion, Massage Cream, Nourishing Cream, Eye Cream, Moisture Cream, Hand Cream, Foundation, Nourishing Essence, Sunscreen, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, Body Lotion and Body Cleanser It may have a formulation selected from the group consisting of, but is not limited thereto. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc acid may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic esters, fatty acid esters of polyethylene glycol or sorbitan, and the like.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

The cosmetic composition obtained from the above method removes active oxygen in the skin by GABA and functional ingredients, promotes collagen biosynthesis, and exhibits melanin production inhibitory activity to provide excellent antioxidant, anti-wrinkle and whitening cosmetics. can

[Example]

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1: Preparation of plant extract

After pulverizing the dried plants shown in Table 1 below, 200 g per 1 L of water was added, and hot water extraction was performed until the solvent became 200 ml.

botanical name English or scientific name botanical name English or scientific name calamansi Kalamansi aloes Aquilaria agallocha Roxburgh sour tree leaves Acer tataricum sub Jeju Joritdae Sasa quelpaertensis Baby Moonlight Outpost Oenothera laciniata Hill Korean thistle Cirsium setidens Peanut Shell Arachis hypogaea black beans Glycine max Merr Blueberries Vaccinium spp. black garlic Allium sativum Linneaus chaga mushroom Inonotus Obliquus

Example 2: Germination of brown rice

Put brown rice in the plant hot water extract prepared in Example 1 alone and mixed (50:50 volume ratio), and supply oxygen using an oxygenator for a water tank at 30-32° C. until the sprouts of brown rice are 2 mm 20 Germination was carried out for ~28 hours.

Example 3: Preparation of germinated brown rice extract with increased GABA content from germinated brown rice

After drying the germinated brown rice prepared in Example 2, purified water (D.W.) 10 times the weight of the crushed powder was added, and the active ingredients were extracted at 40° C. for 8 hours to prepare a germinated brown rice extract. At this time, as a control, extracts of brown rice and germinated brown rice germinated in water were also prepared.

Example 4: Method for measuring GABA content

1 g of the dried product of the germinated brown rice extract prepared in Example 3 was added, 10 mL of 6N hydrochloric acid was added, and then hydrolyzed at 110° C. for 22 hours. After filtering this with filter paper, take 10uL for derivatization, add 70uL of Borate Buffer and 20uL of AccQ-Fluor Reagent, mix well for 10 seconds, leave for 1 minute, heat in a 55℃ oven for 10 minutes, cool, and use as the final test solution and analyzed by HPLC (Alliance 2695, Waters, USA). The analysis conditions are as follows, and for the preparation of the standard solution, GABA is dissolved in distilled water and diluted in each step, and the GABA content is measured from the standard calibration curve prepared using the standard solution, and the results are shown in Table 2.

Detector: Waters 2475 Fluorescence detector

Column : AccQ Taq ^TM (3.9×150mm)

Flow: 1.0 mL/min

Column temperature: 30℃

Mobile Phase: A - Waters AccQ Taq Eluent A: D.W. = 1:10; B - Acetonitrile; C - tertiary distilled water

sample GABA (mg/100g) Brown rice 3.104 Germinated brown rice soaked in water 8.350 Germinated brown rice with calamansi extract 21.021 Spearmint Leaf Extract Germinated Brown Rice 19.097 Germinated Brown Rice with Baby Dalmatian Herb Extract 23.388 Peanut Shell Extract Germinated Brown Rice 20.012 Blueberry Extract Germinated Brown Rice 17.423 Germinated Brown Rice with Chaga Mushroom Extract 16.779 Germinated brown rice with aloes root extract 28.122 Germinated brown rice with Jeju bamboo shoot extract 22.321 Korean thistle extract germinated brown rice 27.726 Black bean extract germinated brown rice 14.714 Black garlic extract germinated brown rice 13.029 Calamansi Extract + Senna Leaf Extract Germinated Brown Rice 20.025 Calamansi Extract + Peanut Shell Extract Germinated Brown Rice 31.339 Calamansi extract + Jeju rice extract germinated brown rice 28. 350

From Table 2, normal brown rice has a GABA content of 3.1 mg/100 g, and germinated brown rice germinated in water is 8.35 mg/100 g, but generally, when germinated in a plant hot water extract, the GABA content is increased, and among them, aloes agarula and Korean thistle , Germinated brown rice germinated from the extracts of daisy, calamansi, peanut husks, cinnamon leaves, and Jeju bamboo shoots significantly increased the GABA content. And it was found.

As a specific part of the present invention has been described in detail above, for those of ordinary skill in the art, it is clear that this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. will be. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

Claims (7)

The GABA content including the step of immersing and germinating brown rice in an immersion water containing one or more plant extracts selected from the group consisting of aloes wood , cinnamon leaf, Jeju sagebrush, Korean thistle, peanut husk and Arabidopsis is increased. A method for producing germinated brown rice.
The method of claim 1, wherein oxygen is supplied when the brown rice is immersed.
(a) immersing and germinating brown rice in an immersion water containing one or more plant extracts selected from the group consisting of aloes tree , cinnamon leaf, Jeju porridge, Korean thistle, peanut shell and Arabidopsis; and
(b) crushed germinated brown rice, then added purified water and stirred at 30 to 60° C. for 6 to 10 hours to prepare a germinated brown rice extract manufacturing method.
The method of claim 3, wherein oxygen is supplied when the brown rice is immersed.
A germinated brown rice extract with an increased GABA content prepared by the method of claim 3 or 4.
A natural cosmetic composition comprising an extract of germinated brown rice with an increased GABA content prepared by the method of claim 3 or 4.
The natural cosmetic composition according to claim 6, wherein the germinated brown rice extract having an increased GABA content is included in an amount of 0.5 to 50 parts by weight based on 100 parts by weight of the natural cosmetic composition.

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