KR102369272B1 - Manufacturing method for cube-type natural Korean beef seasoning using roasting mushroom - Google Patents

Manufacturing method for cube-type natural Korean beef seasoning using roasting mushroom Download PDF

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KR102369272B1
KR102369272B1 KR1020190172883A KR20190172883A KR102369272B1 KR 102369272 B1 KR102369272 B1 KR 102369272B1 KR 1020190172883 A KR1020190172883 A KR 1020190172883A KR 20190172883 A KR20190172883 A KR 20190172883A KR 102369272 B1 KR102369272 B1 KR 102369272B1
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powder
mushrooms
cube
roasted
korean beef
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KR20210080860A (en
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김인경
강혜주
김미향
정경임
박미화
김보경
류지혜
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00Meat products; Meat meal; Preparation or treatment thereof
    • A23L13/10Meat meal or powder; Granules, agglomerates or flakes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P30/00Shaping or working of foodstuffs characterised by the process or apparatus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/20Ingredients acting on or related to the structure

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Abstract

본 발명은 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법에 관한 것이다. 보다 상세하게는 표고버섯을 로스팅 처리하여 항산화 및 항염증 효과를 증대시키고, 맛, 냄새, 풍미, 감칠맛이 우수한 한우조미료를 제조할 수 있도록 하며, 정량화된 1인분의 고상의 큐브형으로 제조하여 유통과정에서 큐브형태가 유지되고 파손되지 않도록 하고, 화학첨가제나 방부제 및 보존제를 첨가하지 않고 수분함유량을 적정 수준으로 조절하여 미생물의 번식을 억제하고 보존력을 향상시키도록 하는 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법에 관한 것이다. The present invention relates to a method for manufacturing a cube-shaped natural Korean beef seasoning using roasted mushrooms. More specifically, the roasting process of shiitake mushrooms increases antioxidant and anti-inflammatory effects, makes it possible to manufacture Korean beef seasoning with excellent taste, odor, flavor, and umami, and manufactures and distributes quantified solid cubes for 1 serving. Cube-shaped natural Korean beef using roasting mushrooms that maintains the cube shape and prevents it from being damaged in the process, and controls the moisture content to an appropriate level without adding chemical additives, preservatives or preservatives to suppress the growth of microorganisms and improve preservation It relates to a seasoning manufacturing method.

Description

로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법{Manufacturing method for cube-type natural Korean beef seasoning using roasting mushroom}Cube-type natural Korean beef seasoning using roasting mushroom {Manufacturing method for cube-type natural Korean beef seasoning using roasting mushroom}

본 발명은 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법에 관한 것이다. 보다 상세하게는 표고버섯을 로스팅 처리하여 항산화 및 항염증 효과를 증대시키고, 맛, 냄새, 풍미, 감칠맛이 우수한 한우조미료를 제조할 수 있도록 하며, 정량화된 1인분의 고상의 큐브형으로 제조하여 유통과정에서 큐브형태가 유지되고 파손되지 않도록 하고, 화학첨가제나 방부제 및 보존제를 첨가하지 않고 수분함유량을 적정 수준으로 조절하여 미생물의 번식을 억제하고 보존력을 향상시키도록 하는 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법에 관한 것이다. The present invention relates to a method for manufacturing a cube-shaped natural Korean beef seasoning using roasted mushrooms. More specifically, the roasting process of shiitake mushrooms increases antioxidant and anti-inflammatory effects, makes it possible to manufacture Korean beef seasoning with excellent taste, odor, flavor, and umami, and manufactures and distributes quantified solid cubes for 1 serving. Cube-shaped natural Korean beef using roasting mushrooms that maintains the cube shape and prevents it from being damaged in the process, and controls the moisture content to an appropriate level without adding chemical additives, preservatives or preservatives to suppress the growth of microorganisms and improve preservation It relates to a seasoning manufacturing method.

국민소득 증대에 따라 아동 비만 및 성인병 발병률이 증가함에 따라 건강에 대한 관심이 증대되고 있다. 이에 따라 화학조미료 사용을 줄이면서 이를 대체할 수 있는 천연조미료 제품에 대한 관심이 높아지고 있는 추세이다.As the incidence of childhood obesity and geriatric diseases increases along with the increase in national income, interest in health is increasing. Accordingly, there is a growing interest in natural seasoning products that can replace chemical seasonings while reducing the use of chemical seasonings.

한편, 1~2인 가구가 증가하면서 1~2인 가구를 위한 편의성을 강조한 소용량 제품에 대한 선호도가 높아지고 있다. 최근 6개월 이내 식품 구입 경험이 있는 성인남녀 1000명을 대상으로 소포장 식품 소비에 관한 조사를 실시한 결과, 소비자의 30~40% 가량이 소용량 포장을 선호하고 있음을 확인할 수 있었다. Meanwhile, as the number of households with one or two members increases, preference for small-capacity products that emphasize convenience for single or two-person households is increasing. As a result of conducting a small-packed food consumption survey on 1,000 adult men and women who have purchased food within the past 6 months, it was found that 30-40% of consumers prefer small-capacity packaging.

또한, 제품의 용량이 필요 이상으로 많아, 정량을 모두 소진하지 못하고 유통기한을 지나치게 되어 이로 인해 소포장 식품을 선호하는 소비자도 점점 늘어나고 있는 추세이다. In addition, the capacity of the product is more than necessary, so the quantity cannot be exhausted and the expiration date is exceeded.

선행기술문헌 : KR 등록특허공보 제0941779호(2010.2.11.공고) Prior art document: KR Registered Patent Publication No. 0941779 (2010.2.11. Announcement)

본 발명은 상기와 같은 문제점을 해결하기 위해 안출된 것으로, 특히 표고버섯이 가지는 항산화 및 항염증능을 증대시키고, 조리시 맛, 냄새, 풍미가 우수한 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법을 제공하는 데 그 목적이 있다.The present invention has been devised to solve the above problems, and in particular, increases the antioxidant and anti-inflammatory properties of shiitake mushrooms, and provides a method for producing a cube-shaped natural Korean beef seasoning using roasted mushrooms having excellent taste, smell and flavor during cooking. Its purpose is to provide

상기 목적을 달성하기 위해 안출된 본 발명에 따른 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법은 (a) 원료를 건조 및 분쇄하는 원료준비단계; (b) 소금, 간장, 설탕, 대파농축분말, 양파농축분말, 마늘농축분말, 효모추출물, 로스팅표고버섯분말, 포도당, 양파분말, 한우소고기분말, 마늘분말, 스테아린산마그네슘, 흑후추분말을 혼합기에 투입하여 배합하는 배합단계; (c) 수분함량을 측정하여 수분함량이 일정 범위를 초과하면 건조기에 투입하여 건조시키고, 수분함량이 일정 범위 미만일 경우 수분을 공급하는 수분함량조절단계; (d) 배합된 원료를 타정기기에 투입하여 타정방식으로 육면체형상으로 성형하는 성형단계; (e) 성형 이후 무게, 크기, 경도를 포함하는 품질검사를 실시하는 경도 및 중량검사단계; 및 (f) 완충기구를 용기 하단에 장착하고 큐브형 천연한우조미료를 투입하여 포장하는 포장단계 를 포함할 수 있다. A method for producing a cube-shaped natural Korean beef seasoning using roasted mushrooms according to the present invention devised to achieve the above object includes: (a) a raw material preparation step of drying and pulverizing the raw material; (b) Salt, soy sauce, sugar, green onion concentrate powder, onion concentrate powder, garlic concentrate powder, yeast extract, roasted shiitake mushroom powder, glucose, onion powder, Korean beef powder, garlic powder, magnesium stearate, black pepper powder are put into the mixer a blending step of blending; (c) a moisture content control step of measuring the moisture content and, when the moisture content exceeds a certain range, put it in a dryer to dry it, and supply moisture when the moisture content is less than a certain range; (d) a molding step of injecting the blended raw material into a tableting machine and molding it into a hexahedral shape by a tableting method; (e) a hardness and weight inspection step of performing quality inspection including weight, size, and hardness after molding; And (f) it may include a packaging step of mounting a buffer mechanism at the bottom of the container and packing by putting the cube-shaped natural Korean beef seasoning.

또한, (a) 원료준비단계는 (a1) 마늘분말을 65℃에서 1차 건조 후 이물 및 불순물을 제거하고 건조기에서 65℃에서 2차 건조 후 파쇄 및 분쇄하여 마늘분말을 제조하는 마늘분말 제조단계, (a2) 표고버섯을 햇빛에서 1~2일간 1차 건조하고, 이물,불순물 선별한 후 건조기에 투입하여 65℃에서 2차 건조한 후 180~200℃의 온도에서 20~45분 간 로스팅을 실시하고, 파쇄 및 분쇄하여 로스팅표고버섯 분말을 제조하는 로스팅표고버섯 분말 제조단계, 및 (a3) 원료를 60℃의 온도에서 1차 건조시킨 후, 원료를 물에 끓여서 추출을 진행하고, 끓이고 잔여한 침전물을 여과 및 농축시킨 후 저온살균과정을 거치고, 건조기에 원료와 알파미분을 투입하여 진공건조시킨 후, 파쇄 및 분쇄하여 농축액분말을 제조하는 농축액분말 제조단계를 포함할 수 있다. In addition, (a) raw material preparation step is (a1) garlic powder manufacturing step in which foreign substances and impurities are removed after primary drying at 65° C., and crushed and pulverized after secondary drying at 65° C. in a dryer to produce garlic powder , (a2) Dry the shiitake mushrooms in sunlight for 1-2 days, select foreign substances and impurities, put them in a dryer, dry them secondarily at 65℃, and roast them at 180-200℃ for 20-45 minutes. The roasting shiitake mushroom powder manufacturing step of producing roasted shiitake mushroom powder by crushing and pulverizing, and (a3) drying the raw material first at a temperature of 60 ° C., boiling the raw material in water to proceed with extraction, boiling and remaining After filtering and concentrating the precipitate, it is subjected to a pasteurization process, and the raw material and alpha fine powder are put in a dryer, dried in a vacuum, and crushed and pulverized to prepare a concentrate powder.

또한, (a1) 내지 (a3)단계는 파쇄 및 분쇄 이후, 쇠가루의 혼입으로 인한 금속성분을 검출하여 제거하는 분급 및 금속제거단계와, 수분활성도를 측정하여 일정 범위 초과인 경우 건조기에 투입하여 진공건조시키는 수분활성도 조절단계를 더 포함할 수 있다. In addition, steps (a1) to (a3) include a classification and metal removal step of detecting and removing metal components due to mixing of iron powder after crushing and pulverization, and measuring water activity and, if exceeding a certain range, put it into a dryer and vacuum It may further include a water activity control step of drying.

본 발명에 의하면 표고버섯을 로스팅 처리하여 항산화 및 항염증 효과를 증대시키고, 맛, 냄새, 풍미, 감칠맛이 우수한 한우조미료를 제조할 수 있도록 하는 데 그 효과가 있다. According to the present invention, it is effective in increasing the antioxidant and anti-inflammatory effects by roasting shiitake mushrooms, and making it possible to prepare a seasoning for Korean beef excellent in taste, odor, flavor and umami.

또한, 본 발명에 의하면, 정량화된 1인분의 고상의 큐브형으로 제조되며 유통과정에서 큐브형태가 유지되고 파손되지 않도록 하는 데 그 효과가 있다. In addition, according to the present invention, it is produced in a quantified solid cube shape for 1 serving, and it is effective in maintaining the cube shape and preventing it from being damaged in the distribution process.

또한, 본 발명에 의하면 화학첨가제나 방부제 및 보존제를 첨가하지 않고, 수분함유량을 적정 수준으로 조절함으로써 미생물의 번식을 억제하여 보존력을 향상시키도록 하는 데 그 효과가 있다. In addition, according to the present invention, without adding chemical additives, preservatives and preservatives, by controlling the moisture content to an appropriate level, it is effective in suppressing the reproduction of microorganisms to improve the preservation power.

도 1은 본 발명의 바람직한 실시예에 따른 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법의 순서도,
도 2는 본 발명의 바람직한 실시예에 따른 완충기구를 도시한 도면,
도 3은 본 발명의 바람직한 실시예에 따른 버섯관능테스트 결과그래프를 도시한 도면,
도 4는 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 DPPH radical 소거능의 그래프를 도시한 도면,
도 5는 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 ABTS radical 소거능의 그래프를 도시한 도면,
도 6은 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 MC3T3-E1세포독성 및 LPS 유도 RAW 264.7세포독성 실험을 실시한 결과그래프를 도시한 도면이다.
1 is a flowchart of a method for manufacturing a cube-shaped natural Korean beef seasoning using roasted mushrooms according to a preferred embodiment of the present invention;
2 is a view showing a buffer mechanism according to a preferred embodiment of the present invention;
3 is a view showing a mushroom sensory test result graph according to a preferred embodiment of the present invention;
Figure 4 is a graph showing the DPPH radical scavenging ability of raw mushrooms, dried mushrooms and roasted mushrooms hot water extracts and ethanol extracts according to a preferred embodiment of the present invention;
5 is a graph showing the ABTS radical scavenging ability of the hot water extract and ethanol extract of raw mushrooms, dried mushrooms and roasted mushrooms according to a preferred embodiment of the present invention;
6 is a graph showing the results of MC3T3-E1 cytotoxicity and LPS-induced RAW 264.7 cytotoxicity experiments of raw, dried and roasted mushroom hot-water extracts and ethanol extracts according to a preferred embodiment of the present invention.

이하, 본 발명의 바람직한 실시예를 첨부된 도면들을 참조하여 상세히 설명한다. 우선 각 도면의 구성 요소들에 참조 부호를 부가함에 있어서, 동일한 구성 요소들에 대해서는 비록 다른 도면상에 표시되더라도 가능한 한 동일한 부호를 가지도록 하고 있음에 유의해야 한다. 또한, 본 발명을 설명함에 있어, 관련된 공지 구성 또는 기능에 대한 구체적인 설명이 본 발명의 요지를 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명은 생략한다. 또한, 이하에서 본 발명의 바람직한 실시예를 설명할 것이나, 본 발명의 기술적 사상은 이에 한정하거나 제한되지 않고 당업자에 의해 변형되어 다양하게 실시될 수 있음은 물론이다.Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. First of all, it should be noted that in adding reference numerals to the components of each drawing, the same components are given the same reference numerals as much as possible even though they are indicated on different drawings. In addition, in describing the present invention, if it is determined that a detailed description of a related known configuration or function may obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, preferred embodiments of the present invention will be described below, but the technical spirit of the present invention is not limited thereto and may be variously implemented by those skilled in the art without being limited thereto.

도 1은 본 발명의 바람직한 실시예에 따른 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법의 순서도이고, 도 2는 본 발명의 바람직한 실시예에 따른 완충기구를 도시한 도면이며, 도 3은 본 발명의 바람직한 실시예에 따른 버섯관능테스트 결과그래프를 도시한 도면이고, 도 4는 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 DPPH radical 소거능의 그래프를 도시한 도면이며, 도 5는 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 ABTS radical 소거능의 그래프를 도시한 도면이고, 도 6은 본 발명의 바람직한 실시예에 따른 생버섯, 건조버섯 및 로스팅 버섯 열수추출물 및 에탄올 추출물의 MC3T3-E1세포독성 및 LPS 유도 RAW 264.7세포독성 실험을 실시한 결과그래프를 도시한 도면이다.1 is a flowchart of a method for manufacturing a cube-shaped natural Korean beef seasoning using roasted mushrooms according to a preferred embodiment of the present invention, FIG. 2 is a view showing a buffer mechanism according to a preferred embodiment of the present invention, and FIG. 3 is the present invention It is a diagram showing a mushroom sensory test result graph according to a preferred embodiment of the present invention, and FIG. 4 is a graph showing the DPPH radical scavenging ability of raw mushrooms, dried mushrooms and roasted mushrooms hot-water extracts and ethanol extracts according to a preferred embodiment of the present invention. 5 is a graph showing the ABTS radical scavenging ability of raw mushrooms, dried mushrooms and roasted mushrooms hot water extracts and ethanol extracts according to a preferred embodiment of the present invention, and FIG. 6 is a raw mushroom according to a preferred embodiment of the present invention, It is a graph showing the results of MC3T3-E1 cytotoxicity and LPS-induced RAW 264.7 cytotoxicity experiments of dried and roasted mushroom hot-water extracts and ethanol extracts.

도 1을 참조하면, 본 발명의 바람직한 실시예에 따른 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법은 원료준비단계(S100), 배합단계(S200), 수분함량조절단계(S300), 성형단계(S400), 경도 및 중량검사단계(S500), 포장단계(S600)를 포함하여 구성된다. Referring to Figure 1, the cube-shaped natural Korean beef seasoning manufacturing method using roasted mushrooms according to a preferred embodiment of the present invention includes a raw material preparation step (S100), a mixing step (S200), a moisture content control step (S300), a molding step ( S400), hardness and weight inspection step (S500), is configured to include a packaging step (S600).

원료준비단계(S100)는 원료를 건조 및 분쇄하는 과정으로, 보다 구체적으로, 원료준비단계(S100)는 마늘분말 제조단계(S110), 로스팅표고버섯 분말 제조단계(S120), 농축분말 제조단계(S130)를 포함한다. The raw material preparation step (S100) is a process of drying and pulverizing the raw materials. More specifically, the raw material preparation step (S100) is a garlic powder manufacturing step (S110), a roasting shiitake mushroom powder manufacturing step (S120), a concentrated powder manufacturing step ( S130).

마늘 및 생강분말 제조단계(S110)는 마늘분말을 65℃에서 8시간 동안 1차 건조 후 이물 및 불순물을 제거하고 건조기에서 65℃에서 1시간 동안 2차 건조 후 파쇄 및 분쇄하여 마늘분말 및 생강분말을 제조하는 과정이다. In the garlic and ginger powder manufacturing step (S110), the garlic powder is first dried at 65° C. for 8 hours, foreign substances and impurities are removed, and then dried in a dryer at 65° C. for 1 hour, then crushed and pulverized to crush and pulverize garlic powder and ginger powder is the process of manufacturing

로스팅표고버섯분말 제조단계(S120)는 햇빛에서 1~2일간 건조하는 1차 건조하고, 이물,불순물 선별한 후 건조기에 투입하고 65℃에서 2시간 건조한 후 180~200℃의 온도에서 20~45분 간 로스팅을 실시하고, 파쇄 후 소정 입도로 분쇄하는 파쇄 및 분쇄하여 로스팅표고버섯분말을 제조하는 과정이다. The roasting shiitake mushroom powder manufacturing step (S120) is first dried in sunlight for 1-2 days, and foreign substances and impurities are selected, then put into a dryer, dried at 65°C for 2 hours, and then dried at a temperature of 180-200°C for 20-45 It is a process of producing roasted shiitake mushroom powder by roasting for minutes, crushing and pulverizing to a predetermined particle size after crushing.

로스팅 조건은 180∼200℃의 온도에서 20~45분 간 실시하는 것이 바람직하며, 180℃ 미만일 경우에는 1시간 이상의 장시간 가열로 표고버섯에 함유된 영양성분이 손실될 우려가 있고, 로스팅 시간이 많이 소요되며, 200℃ 초과일 경우에는 탄맛이 강하게 발현되고 표고버섯에 함유된 영양성분이 손실될 우려가 있다. Roasting conditions are preferably carried out at a temperature of 180-200 ℃ for 20-45 minutes, and if it is less than 180 ℃, there is a risk of loss of nutrients contained in shiitake mushrooms due to prolonged heating of 1 hour or more, and the roasting time is long. If it is over 200℃, the burnt taste is strongly expressed and there is a risk of loss of nutrients contained in shiitake mushrooms.

농축분말 제조단계(S130)는 원료를 60℃의 온도에서 20분~1시간 동안 1차 건조시킨 후, 원료를 물에 끓여서 추출을 진행하고, 끓이고 잔여한 침전물을 여과시킨 후, 40~50 brix로 농축시킨 후, 저온살균과정을 거치고, 건조기에 저온살균과정을 거친 원료와 알파미분을 투입하여 진공건조시킨 후, 1차 파쇄한 원료를 소정의 입도로 2차 분쇄하는 과정이다. In the concentrated powder manufacturing step (S130), the raw material is first dried at a temperature of 60 ° C. for 20 minutes to 1 hour, the raw material is boiled in water to proceed with extraction, and after boiling and filtering the remaining precipitate, 40-50 brix It is a process of secondly grinding the primary crushed raw material to a predetermined particle size after concentrating it with a vacuum cleaner, undergoing a pasteurization process, putting the raw material that has undergone the pasteurization process and alpha fine powder in a dryer, and drying it in a vacuum.

농축분말 제조단계(S130)에 의해 제조되는 농축분말은 대파농축액, 양파농축액, 마늘농축액을 액상에서 고상으로 가공하여 분쇄처리함으로써 분말화 상태로 제조되는 분말이다. The concentrated powder produced by the concentrated powder manufacturing step (S130) is a powder prepared in a powdered state by processing green onion concentrate, onion concentrate, and garlic concentrate from a liquid phase to a solid phase and pulverizing them.

이때, 알파미분은 액상의 농축액을 분말화하기 위한 고상 형태로 가공하기 위해 사용되고, 포도당(정제포도당)은 원료 간의 결착력을 높여주어 성형단계에서 타정이 이루어질 때 깨짐현상을 방지하기 위해 사용된다. At this time, the alpha fine powder is used to process the liquid concentrate into a solid form for pulverizing, and glucose (refined glucose) is used to increase the binding force between the raw materials to prevent breakage when tableting is performed in the molding step.

여기서, 알파미분은 알파화한 쌀가루로, 찌거나 삶은 다음 건조시킨 분말화된 상태의 쌀을 뜻한다. Here, the alpha fine powder is alpha rice flour, and refers to rice in a powdered state that has been steamed or boiled and then dried.

마늘분말 제조단계(S110), 로스팅표고버섯 분말 제조단계(S120), 농축분말 제조단계(S130)는 분쇄이후 분급 및 금속제거단계와 수분활성도 조절단계가 더 수반된다. Garlic powder production step (S110), roasted shiitake mushroom powder production step (S120), and concentrated powder production step (S130) are further accompanied by a classification and metal removal step and a water activity control step after grinding.

보다 구체적으로, 분급 및 금속제거단계는 분쇄시 쇠가루의 혼입으로 인한 금속성분을 검출 및 제거하기 위하여 7000gauss 이상의 봉자석을 이용하여 금속이물질을 검출하여 제거한다.More specifically, in the classification and metal removal step, metal foreign substances are detected and removed using a bar magnet of 7000 gauss or more in order to detect and remove metal components due to the mixing of iron powder during grinding.

또한, 분급 및 금속제거단계에서는 14mesh망으로 이루어진 여과망이 구비되어 여과망을 통과할 수 있는 입도를 가진 원료가 본 공정을 수행할 수 있도록 한다.In addition, in the classification and metal removal step, a filtration net made of 14 mesh is provided so that raw materials having a particle size that can pass through the filtration net can perform this process.

수분활성도 조절단계는 수분활성도를 측정하여 일정 범위 초과인 경우 건조기에 투입하여 진공건조시킨다.In the water activity control step, the water activity is measured and, if it exceeds a certain range, it is put into a dryer and vacuum dried.

보다 구체적으로, 수분활성도 조절단계는 수분활성도를 측정하여 수분활성도가 0.5를 초과하는 경우 물온도 87~90℃, 내부온도 75~80℃, 진공도 700mmAq에서 1시간 내지 1시간10분 동안 건조실시 후 분급 및 금속제거단계를 거친 후 수분활성도를 재측정한다.More specifically, in the water activity control step, when the water activity exceeds 0.5 by measuring the water activity, the water temperature is 87~90℃, the internal temperature is 75~80℃, the vacuum degree is 700mmAq After drying for 1 hour to 1 hour and 10 minutes After the classification and metal removal steps, the water activity is measured again.

수분활성도는 미생물의 생장에 영향을 주는 기본환경조건 중 하나로 물이나 수분의 가용성을 나타내는 것으로 순수한 물의 수증기압에대한 용액의 수증기압의 비율로 표현된다.Water activity is one of the basic environmental conditions that affect the growth of microorganisms and indicates the solubility of water or moisture, and it is expressed as the ratio of the water vapor pressure of a solution to the water vapor pressure of pure water.

대부분의 육상 세균은 최소 생장조건으로 0.91 이상의 수분활성도가 필요하며, 균류(fungi)는 0.86 정도의 낮은 수분활성도에서도 생존이 가능한 것으로 보고되고 있다. 일반적으로 세균, 효모, 곰팡이의 순으로 낮은 수분활성도에서도 잘 견딘다. 하지만 수분활성도가 0.50 이하에서는 미생물의 증식 자체가 거의 불가능하다고 알려져 있다.It has been reported that most terrestrial bacteria require a water activity of 0.91 or higher as a minimum growth condition, and that fungi can survive even at a water activity as low as 0.86. In general, it tolerates low water activity well in the order of bacteria, yeast, and mold. However, it is known that the growth of microorganisms itself is almost impossible when the water activity is less than 0.50.

본 발명의 원료는 수분활성도를 0.5 미만으로 유지하여 화학보존제나 방부제 없이 수분활성도를 낮춰 미생물의 증식을 억제하여 부패를 방지하여 보존력을 향상시킨다.The raw material of the present invention maintains water activity below 0.5 to suppress the growth of microorganisms by lowering water activity without chemical preservatives or preservatives to prevent spoilage, thereby improving preservation.

배합단계(S200)는 소금, 간장, 설탕, 대파농축분말, 양파농축분말, 마늘농축분말, 효모추출물, 로스팅표고버섯분말, 포도당, 양파분말, 한우소고기분말, 마늘분말, 스테아린산마그네슘, 흑후추분말을 혼합기에 투입하여 배합하는 과정이다. In the mixing step (S200), salt, soy sauce, sugar, green onion concentrate powder, onion concentrate powder, garlic concentrate powder, yeast extract, roasted shiitake mushroom powder, glucose, onion powder, Korean beef powder, garlic powder, magnesium stearate, black pepper powder It is a process of mixing by putting it in a mixer.

보다 바람직하게는, 배합단계(S200)는 소금 32~38 중량%, 설탕 13~18 중량%, 포도당(정제포도당) 1~5 중량%, 간장 1~5 중량%, 대파농축액 1~5 중량%, 양파농축액 1~5 중량%, 마늘농축액 1~5 중량%, 양파분말 1~5 중량%, 마늘 분말 1~5 중량%, 로스팅 표고버섯분말 3~8 중량%, 스테아린산마그네슘 1~5 중량%, 효모추출물 3~8 중량%, 흑후추분말 1~5 중량, 한우소고기분말 1~5 중량% 로 배합된다. More preferably, the blending step (S200) is 32 to 38% by weight of salt, 13 to 18% by weight of sugar, 1 to 5% by weight of glucose (refined glucose), 1 to 5% by weight of soy sauce, 1 to 5% by weight of green onion concentrate , Onion concentrate 1 to 5% by weight, garlic concentrate 1 to 5% by weight, onion powder 1 to 5% by weight, garlic powder 1 to 5% by weight, roasted shiitake mushroom powder 3 to 8% by weight, magnesium stearate 1 to 5% by weight , 3 to 8% by weight of yeast extract, 1 to 5% by weight of black pepper powder, and 1 to 5% by weight of Korean beef powder.

마늘분말은 분말화된 원료 간의 결착력을 높여주어 성형단계에서 타정이 용이하게 이루어질 수 있도록 하기 위해 사용된다. Garlic powder is used to increase the binding force between the powdered raw materials to facilitate tableting in the molding step.

스테아린산마그네슘은 성형단계에서 타정이 이루어질 때 타정기기에 원료가 들러붙는 현상을 방지하기 위해 사용된다. Magnesium stearate is used to prevent the material from sticking to the tableting machine when tableting is made in the molding step.

효모추출물은 버텍스IG를 포함하고, 효모추출물은 감칠맛을 부여한다.Yeast extract contains Vertex IG, and yeast extract gives umami taste.

수분함량조절단계(S300)는 배합된 원료의 수분함량을 측정하는 단계로, 측정된 수분함량이 일정 범위를 초과하면 건조기에 투입하여 건조시키고, 수분함량이 일정 범위 미만일 경우 수분을 공급하는 과정이다. The moisture content control step (S300) is a step of measuring the moisture content of the blended raw material. When the measured moisture content exceeds a certain range, it is put into a dryer to dry, and when the moisture content is less than a certain range, moisture is supplied. .

수분함량의 범위는 2~3 중량% 으로 이루어지는 것이 바람직하며, 수분함량이 2 중량% 미만일 경우, 성형단계(S400)에서 타정 시 원료가 부스러지고, 원료 간의 결착력이 떨어질 우려가 있고, 수분함량이 3 중량% 초과할 경우, 세균 번식이 용이하여 보존력이 떨어질 우려가 있으므로, 상술한 범위를 유지하는 것이 바람직하다. It is preferable that the range of the moisture content consists of 2-3 wt%, and when the moisture content is less than 2 wt%, the raw material is crushed during tableting in the forming step (S400), there is a risk that the binding force between the raw materials is reduced, and the moisture content is If it exceeds 3% by weight, it is preferable to maintain the above-described range, because there is a risk of bacterial propagation is easy and there is a possibility that the preservation power may be deteriorated.

성형단계(S400)는 배합된 원료를 소정의 압력을 가하여 큐브형상으로 제조하는 과정이다. The forming step (S400) is a process of manufacturing the blended raw material into a cube shape by applying a predetermined pressure.

보다 구체적으로, 성형단계(S400)는 배합된 원료를 타정기기에 투입하여 타정방식으로 육면체형상으로 성형한다.More specifically, in the forming step (S400), the blended raw material is put into a tableting machine and molded into a hexahedral shape by a tableting method.

이때, 원료의 수분함유량은 2~3 중량% 으로 유지하는 것이 바람직하며, 수분함유량이 2 중량% 미만이면 결착력이 감소하여 성형시 부스러지는 현상이 발생되고, 3 중량% 초과하면 점성이 증가하여 타정기기와의 들러붙는 현상이 발생되어 생산효율이 감소된다.At this time, it is preferable to maintain the moisture content of the raw material at 2 to 3% by weight. If the moisture content is less than 2% by weight, the binding force decreases and crumbling occurs during molding. If it exceeds 3% by weight, the viscosity increases and the tableting machine The sticking phenomenon of the tile occurs and the production efficiency is reduced.

성형시 마늘분말은 다른분말들과의 결착력을 높여주어 성형에 용이하게 하며, 포도당(정제포도당)은 성형시 경도를 향상시켜 깨짐현상을 방지해주는 기능을 한다.During molding, garlic powder enhances bonding with other powders to facilitate molding, and glucose (refined glucose) improves hardness during molding and prevents cracking.

스테아린산마그네슘은 성형단계에서 타정기기로 타정이 이루어 질때 타정기기와 분말원료간의 들러붙는 현상을 방지해주는 기능을 한다.Magnesium stearate prevents the adhesion between the tableting machine and the powder raw material when the tablet is made with a tableting machine in the molding stage.

본 발명에 의해 제조된 큐브형상의 천연조미료는 1인용 분량으로 고형화되어 조미료의 정량을 사용하기에 용이하고, 보관 및 휴대가 간편하여 물에 대한 용해도도 우수하다.The cube-shaped natural seasoning prepared according to the present invention is solidified in a single serving, so it is easy to use a fixed amount of the seasoning, and it has excellent solubility in water because it is easy to store and carry.

경도 및 중량검사단계(S500)는 성형단계(S400) 이후 무게, 크기, 경도를 포함하는 품질검사를 실시하는 과정이다. The hardness and weight inspection step (S500) is a process of performing a quality inspection including weight, size, and hardness after the molding step (S400).

성형단계(S400)에서 성형된 큐브형상의 천연조미료는 한 모서리의 길이가 12mm 내지 15mm 로 이루어지며, 무게는 2 내지 3g 으로 형성되며, 이러한 용량은 물 330ml에 큐브형상의 천연조미료 하나가 용해되어 1인분 분량의 정량의 조미료를 사용하기에 용이하다. 여기서 큐브형상의 천연조미료의 규격은 이에 한정하는 것은 아니며, 천연조미료의 조성 및 배합비율에 따라 변경되어 사용될 수 있다.The cube-shaped natural seasoning molded in the forming step (S400) has a length of 12 mm to 15 mm at one edge, and a weight of 2 to 3 g. This capacity is obtained by dissolving one cube-shaped natural seasoning in 330 ml of water. It is easy to use the seasoning in the quantity for 1 serving. Here, the specification of the cube-shaped natural seasoning is not limited thereto, and it may be changed and used according to the composition and mixing ratio of the natural seasoning.

포장단계(S600)에서는 완충기구를 용기 하단에 장착하고 큐브형 천연한우조미료를 투입하여 포장하는 과정으로, 용기 내부에 완충기구가 구비되고, 상기 완충기구는 소정 간격을 두고 다수개의 홀이 구비되며, 연성 재질로 구성되어 내부에 실리카겔을 포함하는 건조제를 수용할 수 있도록 한다. In the packaging step (S600), a buffer mechanism is mounted at the bottom of the container and a cube-shaped natural Korean beef seasoning is put in and packaged. A buffer mechanism is provided inside the container, and the buffer mechanism is provided with a plurality of holes at predetermined intervals. , it is composed of a soft material to accommodate the desiccant containing silica gel inside.

보다 구체적으로, 포장단계(S600)에서 용기 하단에 구비되는 완충기구는 형태학적으로 육면체의 큐브형상은 운반 시 파손의 위험이 있어 이러한 파손을 최소한으로 줄일 수 있다.More specifically, in the packaging step (S600), the buffer mechanism provided at the bottom of the container has a morphologically hexahedral cube shape that has a risk of damage during transport, so that such damage can be minimized.

완충기구는 돔형의 형태로 제품의 운반 시 큐브형상의 조미료가 용기의 내부에서 흔들리며 서로 부딪치며 파손되는 것을 방지할 수 있다.The buffer mechanism is in the form of a dome and can prevent the cube-shaped seasoning from collided with each other and damaged when transporting the product while shaking inside the container.

도 2를 참조하면, 완충기구는 연성 재질로 이루어지고, 돔형상으로 다수개의 홀이 구비되며 홀의 크기는 돔형상의 상부에서 하부로 갈수록 점차 커지는 형상으로 되어 충격을 효과적으로 흡수할 수 있다.Referring to FIG. 2 , the buffer mechanism is made of a soft material, a plurality of holes are provided in a dome shape, and the size of the hole gradually increases from the upper part of the dome shape to the lower part, so that the shock can be effectively absorbed.

1. 표고버섯 관능테스트1. Shiitake Mushroom Sensory Test

도 3은 표고버섯, 느타리버섯, 팽이버섯, 양송이버섯을 건조하여 동일한 량(30g)으로 육수를 낸 뒤 관능검사를 진행하였으며, 평가항목은 맛, 냄새, 풍미, 감칠맛으로 전체적인 기호도로 7점 척도법을 사용하여 실시하였다. 3 is a sensory test after drying shiitake mushrooms, oyster mushrooms, enoki mushrooms, and shiitake mushrooms in the same amount (30 g), and the evaluation items were taste, smell, flavor, and umami. was carried out using

도 3을 참조하면, 맛, 냄새, 감칠말, 풍미 전반적으로 표고버섯이 관능테스트에서 높은 점수로 나왔으며 버섯 중에 육수로 사용하기에 표고버섯이 적합한 것으로 판단되었다. Referring to FIG. 3 , shiitake mushrooms showed high scores in the sensory test overall in taste, smell, taste, and flavor, and it was determined that shiitake mushrooms were suitable for use as broth among mushrooms.

2. 건표고버섯과 로스팅버섯 관능테스트2. Sensory test of dried shiitake and roasted mushrooms

아래의 표 1은 건표고버섯과 로스팅표고버섯의 관능적 특성을 평가한 결과로, 7점 척도를 기준으로 맛, 풍미 및 감칠맛의 관능테스트를 진행하였다. Table 1 below is the result of evaluating the sensory characteristics of dried shiitake mushrooms and roasted shiitake mushrooms, and sensory tests of taste, flavor and umami were conducted based on a 7-point scale.

관능테스트sensory test 건표고버섯dried shiitake mushrooms 로스팅표고버섯Roasted Shiitake Mushrooms 건조기 65℃ 24시간Dryer 65℃ 24 hours 1차 햇빛건조
2차 건조기 65℃ 2시간
1st sunlight drying
Secondary dryer 65℃ 2 hours
200℃ 20분200℃ 20min 180℃ 45분180℃ 45min
taste 4.24.2 6.16.1 3.03.0 5.95.9 냄새smell 4.64.6 5.35.3 4.24.2 5.65.6 풍미zest 4.34.3 5.15.1 4.64.6 5.35.3

표 1을 참조하면, 관능테스트 실시결과, 건조표고버섯은 1차 햇빛 건조 2차 건조기 65℃ 2시간 건조한 것과 로스팅표고버섯은 1차 햇빛 건조, 2차 건조기 65℃ 2시간 건조 후 180℃ 45분 로스팅 조건이 맛, 냄새, 풍미 평가항목에서 우수한 결과를 나타내었다. Referring to Table 1, as a result of the sensory test, dried shiitake mushrooms were first dried in sunlight at 65°C for 2 hours in the secondary dryer, and roasted shiitake mushrooms were dried in the first sunlight at 65°C for 2 hours and dried at 65°C in the second dryer for 2 hours at 180°C 45 minutes. The roasting conditions showed excellent results in taste, odor, and flavor evaluation items.

3. 항산화활성 측정 및 함량결과3. Antioxidant activity measurement and content result

① 총 폴리페놀 함량 측정① Measurement of total polyphenol content

총 polyphenol 함량은 Folin-Ciocalteu법을 변형시켜 측정하였으며 표준물질로는 tannic acid (Sigma Chemical Co., St. Louis, Mo, USA)를 사용하여 분석하였다. The total polyphenol content was measured by modifying the Folin-Ciocalteu method and analyzed using tannic acid (Sigma Chemical Co., St. Louis, Mo, USA) as a standard material.

시료 1 mL에 95% 에탄올 1 mL, 정제수 5 mL 및 50% Folin-ciocalteu reagent (Sigma) 0.5 mL을 혼합하여 5분간 방치 후 5 Na2CO3 1 mL를 첨가하여 교반시킨 다음 1시간 동안 암소에서 반응 후 gallic acid를 표준시약으로 사용하여 760 nm에서 흡광도 측정하였다. To 1 mL of sample, mix 1 mL of 95% ethanol, 5 mL of purified water, and 0.5 mL of 50% Folin-ciocalteu reagent (Sigma), stand for 5 minutes, add 1 mL of 5 Na2CO3, stir, and then react in a dark place for 1 hour. The absorbance was measured at 760 nm using acid as a standard reagent.

② 총 폴리페놀 함량 ② Total polyphenol content

약용식물에 널리 분포되어 있는 폴리페놀 화합물들은 다양한 기능을 하는 파이토케미칼(phytochemical)로서 항산화작용과 항돌연변이 등의 생리활성이 있는 것으로 알려져 있다. Polyphenol compounds widely distributed in medicinal plants are phytochemicals with various functions and are known to have physiological activities such as antioxidant action and anti-mutation.

식물체의 주요 생리활성물질에는 플라보노이드, 탄닌, 안토시아닌, 페놀산 및 카테킨 등의 페놀성 성분이 있으며, 대부분은 산화, 항균 활성 및 항암작용 등의 활성을 지니고 식물의 고유색깔을 띠게 하거나 떫은맛과 쓴맛 등의 특유한 맛을 내는 2차 대사산물로써 작용한다. The main physiologically active substances of plants include phenolic components such as flavonoids, tannins, anthocyanins, phenolic acids, and catechins, and most of them have activities such as oxidation, antibacterial activity and anticancer activity, giving the plant its own color, astringent and bitter taste, etc. It acts as a secondary metabolite that gives the characteristic taste of

인체의 세포 손상과 노화에 의한 질병은 생체 내에서 생성되는 자유기(free radical)에 의해 발생되는데, 페놀 화합물들은 활성산소 불활성화 및 지질과산화물이 활성산소로의 분해를 저해함으로써 항산화력을 나타내는 매우 효과적인 활성산소 제거제인 것으로 알려져 있다. Cell damage in the human body and diseases caused by aging are caused by free radicals generated in the body. Phenolic compounds exhibit antioxidant power by inactivating active oxygen and inhibiting the decomposition of lipid peroxide into active oxygen. It is known to be an effective free radical scavenger.

특히 버섯은 catechin, quercetin, rutin 및 naringin 등을 함유하고 있다고 알려져 있으며, 유해산소를 없애고 염증을 막아주는 등 천연 항산화제의 역할을 가진다고 알려져 있다. In particular, mushrooms are known to contain catechin, quercetin, rutin, and naringin, and are known to have the role of natural antioxidants, such as removing free radicals and preventing inflammation.

본 실험에서 생버섯, 건조버섯 및 로스팅버섯 열수 및 에탄올 추출물의 폴리페놀 함량을 검토한 결과, 열수와 에탄올 추출물 모두에서 로스팅 버섯이 유의적으로 높은 것으로 나타났다. As a result of examining the polyphenol content of raw mushrooms, dried mushrooms and roasted mushrooms in hot water and ethanol extracts, it was found that roasted mushrooms were significantly higher in both hot water and ethanol extracts.

표 2는 생버섯, 건조버섯 및 로스팅 버섯 열수 및 에탄올 추출물의 폴리페놀 함량을 나타낸 것으로, 표 2를 참조하면, 생버섯, 건조버섯 및 로스팅버섯 열수 추출물의 총 폴리페놀 함량은 각각 5.54 mg/g GAE와 5.56 mg/g GAE 및 8.20 mg/g GAE로 로스팅버섯의 총 폴리페놀 함량이 높은 것으로 나타났고, 에탄올 추출물은 각각 4.92 mg/g GAE와 5.01 mg/g GAE 및 8.40 mg/g GAE으로 로스팅버섯의 총 폴리페놀 함량이 높은 것으로 나타났다. Table 2 shows the polyphenol content of raw, dried and roasted mushroom hot water and ethanol extract. Referring to Table 2, the total polyphenol content of raw mushroom, dried mushroom and roasted mushroom hot water extract was 5.54 mg/g GAE and The total polyphenol content of roasted mushrooms was high with 5.56 mg/g GAE and 8.20 mg/g GAE, and the ethanol extract contained 4.92 mg/g GAE, 5.01 mg/g GAE, and 8.40 mg/g GAE, respectively, of roasted mushrooms. The total polyphenol content was found to be high.

(단위: mg/g GAE)(Unit: mg/g GAE) 시료sample 생버섯raw mushrooms 건조버섯dried mushrooms 로스팅버섯roasted mushrooms 열수 추출물hot water extract 5.54±0.42b 5.54±0.42 b 5.56±0.10b 5.56±0.10 b 8.20±1.43a 8.20±1.43 a 에탄올 추출물ethanol extract 4.92±1.27b 4.92±1.27 b 5.01±0.24b 5.01±0.24 b 8.40±0.70a 8.40±0.70 a

③ DPPH 라디칼 소거능 측정 ③ Measurement of DPPH radical scavenging ability

Blois의 방법에 따라 DPPH (1,1-Diphenyl-2-picrylhydrazyl)에 대한 수소공여능효과로 측정하였다. According to the method of Blois, the effect of hydrogen donating ability on DPPH (1,1-Diphenyl-2-picrylhydrazyl) was measured.

시료를 에탄올에 녹여 농도별로 희석하여 시료 50 μL와 에탄올을 녹인 1.5×10-4M DPPH 200 μL를 혼합하여 30분 동안 반응 시킨 다음 ELISA reader를 이용하여 520 nm에서 흡광도 측정하여 다음 식에 의하여 분석하였다. Dissolve the sample in ethanol and dilute it by concentration, mix 50 μL of the sample and 200 μL of 1.5×10 -4 M DPPH in ethanol, and react for 30 minutes, then measure the absorbance at 520 nm using an ELISA reader and analyze it by the following formula did

DPPH radical scavenging activity (%) DPPH radical scavenging activity (%)

= (1-시료 첨가구의 흡광도/시료 무첨가구 흡광도) × 100= (1 - Absorbance of the sample addition / Absorbance of the non-sample area) × 100

④ DPPH 라디칼 소거능 결과④ DPPH radical scavenging activity result

항산화효과의 지표로 전자공여능이 사용되고 있으며, DPPH는 비교적 안정한 자유기를 가지고 있는 화합물로 항산화물질과 만나게 되면 환원작용에 의해 radical이 소거되어 탈색되는 점을 이용하여 항산화효과를 측정하는 방법으로 환원력이 클수록 항산화능이 높다. Electron-donating ability is used as an indicator of antioxidant effect. DPPH is a compound with relatively stable free radicals. When it comes into contact with antioxidants, radicals are removed by reducing action and discoloration is used to measure the antioxidant effect. It has high antioxidant capacity.

도 4를 참조하면, 생버섯, 건조버섯 및 로스팅버섯의 DPPH radical 소거능을 측정한 결과, 열수 추추물에서는 세 가지 시료 모두 농도의존적인 증가를 보였으며, 로스팅버섯 열수추출물은 1,000 μg/ml 농도에서 94 %의 최대 활성을 보였으며, 에탄올 추출물에서도 로스팅 추출물이 가장 높은 활성을 보였다. Referring to FIG. 4, as a result of measuring the DPPH radical scavenging ability of raw, dried and roasted mushrooms, all three samples showed a concentration-dependent increase in the hot-water extracts, and the hot-water extracts of roasted mushrooms showed a concentration-dependent increase of 94 at a concentration of 1,000 μg/ml. %, and the roasting extract showed the highest activity even in the ethanol extract.

따라서, 건조 등의 가공공정 중 특히 로스팅 가공을 거친 표고버섯이 DPPH radical 소거능 증가에 영향을 미칠 수 있을 것으로 사료된다. Therefore, it is considered that shiitake mushrooms, which have been specially roasted during processing such as drying, may affect the increase in DPPH radical scavenging ability.

⑤ ABTS 양이온(ABTS++)소거능 측정⑤ ABTS cation (ABTS++) scavenging ability measurement

ABTS 라디칼 소거능은 Lee 등의 방법을 일부 변형하여 측정하였다. ABTS radical scavenging ability was measured by partially modifying the method of Lee et al.

7 mM ABTS 용액과 2.45 mM potassium persulfate을 혼합하여 15시간 동안 암소에 방치하여 ABTS++을 형성시킨 후 30℃에서 온도 평형을 실시한 다음 734 nm에서 흡광도를 측정하여 값이 1.5가 되도록 distilled water로 희석한 다음 희석한 용액에 시료를 혼합하여 실온에서 30분간 반응시킨 후 734 nm에서 흡광도를 측정하였다. 7 mM ABTS solution and 2.45 mM potassium persulfate were mixed and left in a dark place for 15 hours to form ABTS++. After temperature equilibration at 30 °C, absorbance was measured at 734 nm and diluted with distilled water so that the value was 1.5. The sample was mixed with the diluted solution and reacted at room temperature for 30 minutes, and then absorbance was measured at 734 nm.

ABTS radicals scavenging activity (%) ABTS radicals scavenging activity (%)

= (1-시료 첨가구의 흡광도/시료 무첨가구 흡광도) × 100= (1 - Absorbance of the sample addition / Absorbance of the non-sample area) × 100

⑥ ABTS 양이온(ABTS++)소거능 측정결과⑥ ABTS cation (ABTS++) scavenging activity measurement result

ABTS radical 소거능은 ABTS와 potassium persulfate가 반응하여 청록색의 ABTS radical을 생성하고, 생성된 ABTS radical을 무색의 물질로 환원되는 성질을 이용한 측정법으로 비극성과 극성 물질 모두에 적용 가능한 장점이 있기에 항산화 활성 측정에 많이 활용되는 분석법이다. ABTS radical scavenging ability is a measurement method using the property that ABTS and potassium persulfate react to generate blue-green ABTS radical and reduce the generated ABTS radical to a colorless substance. It is a widely used analysis method.

도 5를 참조하면, 버섯, 건조버섯 및 로스팅버섯의 ABTS radical 소거능을 측정한 결과, 열수추출물에서는 농도 의존적인 증가를 보였으며, 1,000 μg/ml 농도에서 80%로 높은 ABTS radical 소거능을 보였으며, 앞선 실험과 동일하게 에탄올 추출물에서도 로스팅 추출물이 가장 높은 활성을 보였다. 5, as a result of measuring the ABTS radical scavenging ability of mushrooms, dried mushrooms and roasted mushrooms, the hot water extract showed a concentration-dependent increase, and showed a high ABTS radical scavenging activity of 80% at a concentration of 1,000 μg/ml, As in the previous experiment, the roasted extract showed the highest activity in the ethanol extract.

4. RAW 264.7 대식세포를 이용한 항염증효과 측정4. Measurement of anti-inflammatory effect using RAW 264.7 macrophages

① 세포 배양 ① Cell culture

RAW 264.7 murine macrophage cell line은 한국 세포주 은행(Seoul, Korea)에서 분양 받아 실험에 사용하였다. 세포 성장을 위한 기본 배지로는 10% FBS, penicillin 100 U/ml, streptomycin 100 μg/ml를 포함하는 DMEM 배지를 사용하였으며, T-flask에 접종하여37℃, 5%의 CO2incubator에서 배양하였다. RAW 264.7 murine macrophage cell line was purchased from the Korean cell line bank (Seoul, Korea) and used in the experiment. As a basic medium for cell growth, DMEM medium containing 10% FBS, penicillin 100 U/ml, and streptomycin 100 μg/ml was used, and inoculated into T-flask and cultured at 37°C, 5% CO2 incubator.

배양된 세포는 2~3일 마다 배지를 교환하면서 실험에 사용하였으며 세포가 80%이상 confluence되었을 때 계대 배양하여 실험에 사용하였다. The cultured cells were used for the experiment while changing the medium every 2-3 days, and when the cells were more than 80% confluent, they were subcultured and used for the experiment.

Nitric Oxide (NO) 생성능 측정 실험에서는 배양된 세포에 시료를 농도별로 첨가하여 2시간 동안 배양한 다음 스트레스를 유발하기 위하여 1 μg/ml의 lipopolysaccharide (LPS)를 첨가하여 20시간 배양하였다. In the experiment for measuring nitric oxide (NO) production capacity, samples were added to the cultured cells by concentration and cultured for 2 hours, and then 1 μg/ml of lipopolysaccharide (LPS) was added to induce stress and incubated for 20 hours.

② 세포독성 측정② Cytotoxicity measurement

RAW 264.7 세포 생존율 측정을 위하여 MTT assay를 실시하였다. MTT assay was performed to measure RAW 264.7 cell viability.

세포가 confluence 상태가 되면 96-well plate에 well당 2×104cells/ml로 세포를 seeding하여 2시간 배양하고, 시료를 농도별로 처리하여 20시간 incubation한 후 배지를 버리고 100ml(1㎎/ml)의 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) solution을 각 well에 주입하여 37℃에서 4시간 재 배양하였다. When the cells reach confluence, seed the cells in a 96-well plate at 2 × 104 cells/ml per well and incubate for 2 hours. After incubating for 20 hours by treating the samples by concentration, discard the medium and use 100 ml (1 mg/ml) of the cells. 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) solution was injected into each well and incubated at 37°C for 4 hours.

Medium에서 MTT solution을 제거하고 100 μl dimethyl sulfoxide (DMSO)를 주입하여 30분 동안 incubation한 후 ELISA plate reader로 550nm에서 흡광도를 측정하였다. After removing the MTT solution from the medium, injecting 100 μl of dimethyl sulfoxide (DMSO) and incubating for 30 minutes, absorbance was measured at 550 nm with an ELISA plate reader.

세포 생존율은 시료의 흡광도를 대조군의 흡광도에 대한 백분율(%)로 나타내었다. Cell viability was expressed as a percentage (%) of the absorbance of the sample relative to the absorbance of the control group.

Cell viability (%) Cell viability (%)

= 대조구 흡광도-시료 첨가구 흡광도/대조구 흡광도×100= Absorbance of control - Absorbance of sample addition / Absorbance of control × 100

③ 세포 독성 결과③ Cytotoxicity result

항산화 실험결과 항산화능이 뛰어난 시료인 로스팅 버섯과 대조군인 생버섯 열수추출물을 이용하여 cell viability룰 측정하였며 MTT 분석법을 통하여 측정하였다. As a result of the antioxidant experiment, the cell viability was measured using the roast mushroom, a sample with excellent antioxidant activity, and the hot water extract of the raw mushroom as a control, and measured by the MTT analysis method.

도 6를 참조하면, 열수추출물의 농도가 50~500 μg/ml 일 때 정상세포인 MC3T3-E1 세포를 이용하여 독성 실험을 수행하였으며, 그 결과 모든 농도에서 독성이 발견되지 않았으며, LPS 유도 RAW264.7 세포의 독성을 실험한 결과에서 열수추출물의 농도가 50~200μg/ml 80%이상의 생존율을 나타내었으며, 로스팅 버섯의 경우 50μg/ml 일 때 약 120%의 최대생존율을 보였으며, 500 μg/ml 농도에서는 76%의 생존율을 보였다. 6, when the concentration of the hot-water extract was 50 to 500 μg/ml, a toxicity experiment was performed using normal cells, MC3T3-E1 cells. As a result, toxicity was not found at any concentration, and LPS-induced RAW264 .7 As a result of the cell toxicity test, the concentration of hot-water extract showed a survival rate of over 80% at 50-200μg/ml, and in the case of roasted mushrooms, the maximum survival rate was about 120% when it was 50μg/ml, and 500 μg/ml ml concentration showed a survival rate of 76%.

이하에서는, 본 발명에 따른 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법에 의해 제조된 천연한우조미료의 조성물 및 조성비율을 달리한 비교예와 실시예를 통해 상세하게 설명한다. Hereinafter, the composition and composition ratio of the natural Korean beef seasoning prepared by the cube-shaped natural Korean beef seasoning using roasting mushrooms according to the present invention will be described in detail through comparative examples and examples.

[비교예 1][Comparative Example 1]

원료Raw material 총함량total content 천일염sea salt 32-38 중량%32-38 wt% rice 15-19 중량%15-19 wt% 맛가마원액간장Makama undiluted soy sauce 1-5 중량%1-5 wt% 유기농설탕organic sugar 15-20 중량%15-20 wt% 효모추출물yeast extract 3-8 중량%3-8 wt% 로스팅표고버섯분말Roasted Shiitake Mushroom Powder 3-8 중량%3-8 wt% 양파분말onion powder 1-5 중량%1-5 wt% 한우소고기분말Hanwoo Beef Powder 5-10 중량%5-10 wt% 영양강화제fortifying 1-5 중량%1-5 wt% 흑후추분말black pepper powder 1-5 중량%1-5 wt%

관능검사결과
(총7점척도)
sensory test result
(total 7-point scale)
taste 3.43.4 냄새smell 5.25.2 풍미zest 4.34.3 전체적기호도overall symbol 3.83.8

[비교예 2][Comparative Example 2]

원료Raw material 총함량total content 천일염sea salt 32-38 중량%32-38 wt% rice 15-19 중량%15-19 wt% 맛가마원액간장Makama undiluted soy sauce 1-5 중량%1-5 wt% 유기농설탕organic sugar 15-20 중량%15-20 wt% 대파농축액green onion concentrate 1-5 중량%1-5 wt% 양파농축액Onion Concentrate 1-5 중량%1-5 wt% 효모추출물yeast extract 3-8 중량%3-8 wt% 로스팅표고버섯분말Roasted Shiitake Mushroom Powder 3-8 중량%3-8 wt% 양파분말onion powder 1-5 중량%1-5 wt% 한우소고기분말Hanwoo Beef Powder 1-5 중량%1-5 wt% 영양강화제fortifying 1-5 중량%1-5 wt% 흑후추분말black pepper powder 1-5 중량%1-5 wt%

관능검사결과
(총7점척도)
sensory test result
(total 7-point scale)
taste 5.85.8 냄새smell 6.16.1 풍미zest 5.45.4 전체적기호도overall symbol 5.85.8

비교예 2는 비교예 1의 조성물 및 조성비율에서 야채의 풍미를 더하기 위하여 대파농축액, 양파농축액을 포함한 야채농축액을 추가하고 한우소고기분말을 줄여 조리 시 다른 식재료와의 조화가 이루어지도록 하였다. In Comparative Example 2, in order to add flavor of vegetables to the composition and composition ratio of Comparative Example 1, vegetable concentrates including green onion concentrate and onion concentrate were added, and Korean beef powder was reduced to achieve harmony with other ingredients during cooking.

그 결과 표 6을 참조하면 관능검사실시결과 비교예 1에 비하여 맛, 냄새, 풍미, 전체적기호도가 모두 우수한 결과를 나타내었다.As a result, referring to Table 6, the sensory test results showed superior results in taste, odor, flavor, and overall preference compared to Comparative Example 1.

[실시예][Example]

원료Raw material 총함량total content 천일염sea salt 32-38 중량%32-38 wt% rice 15-19 중량%15-19 wt% 맛가마원액간장Makama undiluted soy sauce 1-5 중량%1-5 wt% 유기농설탕organic sugar 13-18 중량%13-18 wt% 대파농축액green onion concentrate 1-5 중량%1-5 wt% 양파농축액Onion Concentrate 1-5 중량%1-5 wt% 마늘농축액garlic concentrate 1-5 중량%1-5 wt% 효모추출물yeast extract 3-8 중량%3-8 wt% 로스팅표고버섯분말Roasted Shiitake Mushroom Powder 3-8 중량%3-8 wt% 포도당glucose 1-5 중량%1-5 wt% 양파분말onion powder 1-5 중량%1-5 wt% 한우소고기분말Hanwoo Beef Powder 1-5 중량%1-5 wt% 마늘분말garlic powder 1-5 중량%1-5 wt% 영양강화제fortifying 1-5 중량%1-5 wt% 흑후추분말black pepper powder 1-5 중량%1-5 wt%

관능검사결과
(총7점척도)
sensory test result
(total 7-point scale)
taste 6.26.2 냄새smell 5.65.6 풍미zest 6.16.1 전체적기호도overall symbol 6.36.3

실시예는 비교예 2의 조성물 및 조성비율에서 설탕의 중량비를 줄이는 대신 포도당을 추가로 하여 성형 시 경도를 향상시키는 효과를 발휘할 수 있도록 하고, 마늘분말을 추가로 하여 다른 분말들과의 결착력을 높여주어 성형을 용이하게 하는 동시에 풍미와 맛을 보다 향상시킬 수 있도록 하였다. In the example of Comparative Example 2, instead of reducing the weight ratio of sugar in the composition and composition ratio, adding glucose to exhibit the effect of improving hardness during molding, and adding garlic powder to increase binding force with other powders It was given to facilitate the molding and at the same time to further improve the flavor and taste.

그 결과, 표 8을 참조하면, 관능검사실시결과 비교예 2에 비하여 맛, 냄새, 풍미, 전체적기호도가 모두 우수한 결과를 나타내었다.As a result, referring to Table 8, the sensory test results showed superior results in taste, odor, flavor, and overall preference compared to Comparative Example 2.

이상의 설명은 본 발명의 기술 사상을 예시적으로 설명한 것에 불과한 것으로서, 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자라면 본 발명의 본질적인 특성에서 벗어나지 않는 범위 내에서 다양한 수정, 변경 및 치환이 가능할 것이다. 따라서, 본 발명에 개시된 실시예 및 첨부된 도면들은 본 발명의 기술 사상을 한정하기 위한 것이 아니라 설명하기 위한 것이고, 이러한 실시예 및 첨부된 도면에 의하여 본 발명의 기술 사상의 범위가 한정되는 것은 아니다. 본 발명의 보호 범위는 아래의 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The above description is merely illustrative of the technical idea of the present invention, and those of ordinary skill in the art to which the present invention pertains may make various modifications, changes and substitutions within the scope without departing from the essential characteristics of the present invention. will be. Accordingly, the embodiments disclosed in the present invention and the accompanying drawings are for explaining, not limiting, the technical spirit of the present invention, and the scope of the technical spirit of the present invention is not limited by these embodiments and the accompanying drawings . The protection scope of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

S100 - 원료준비단계 S200 - 배합단계
S300 - 수분함량조절단계 S400 - 성형단계
S500 - 경도 및 중량검사단계 S600 - 포장단계
S100 - Raw material preparation stage S200 - Mixing stage
S300 - Moisture content control step S400 - Molding step
S500 - Hardness and weight inspection step S600 - Packaging step

Claims (3)

(a) 원료를 건조 및 분쇄하는 원료준비단계;
(b) 소금, 간장, 설탕, 대파농축분말, 양파농축분말, 마늘농축분말, 효모추출물, 로스팅표고버섯분말, 포도당, 양파분말, 한우소고기분말, 마늘분말, 스테아린산마그네슘, 흑후추분말을 혼합기에 투입하여 배합하는 배합단계;
(c) 수분함량을 측정하여 수분함량이 일정 범위를 초과하면 건조기에 투입하여 건조시키고, 수분함량이 일정 범위 미만일 경우 수분을 공급하는 수분함량조절단계;
(d) 배합된 원료를 타정기기에 투입하여 타정방식으로 육면체형상으로 성형하는 성형단계;
(e) 성형 이후 무게, 크기, 경도를 포함하는 품질검사를 실시하는 경도 및 중량검사단계; 및
(f) 완충기구를 용기 하단에 장착하고 큐브형 천연한우조미료를 투입하여 포장하는 포장단계
를 포함하고,
(f) 포장단계에서 이용되는 완충기구는
소정 간격을 두고 다수 개의 홀이 구비되며,
홀의 크기는 상부에서 하부로 갈수록 점차 커지는 형상으로 형성되며,
연성 재질로 이루어지고,
내부에 건조제를 수용할 수 있는 공간이 마련되며,
전체적인 형상이 돔 형상으로 형성되는 것
을 포함하는 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법.
(a) a raw material preparation step of drying and pulverizing the raw material;
(b) Salt, soy sauce, sugar, green onion concentrate powder, onion concentrate powder, garlic concentrate powder, yeast extract, roasted shiitake mushroom powder, glucose, onion powder, Korean beef powder, garlic powder, magnesium stearate, black pepper powder are put into the mixer a blending step of blending;
(c) a moisture content control step of measuring the moisture content and, when the moisture content exceeds a certain range, put it in a dryer to dry it, and supply moisture when the moisture content is less than a certain range;
(d) a molding step of putting the blended raw material into a tableting machine and molding it into a hexahedral shape by a tableting method;
(e) a hardness and weight inspection step of performing quality inspection including weight, size, and hardness after molding; and
(f) A packaging step in which a buffer mechanism is installed at the bottom of the container and cube-shaped natural Korean beef seasoning is put in and packaged
including,
(f) The buffer mechanism used in the packaging step shall be
A plurality of holes are provided at predetermined intervals,
The size of the hole is formed in a shape that gradually increases from the top to the bottom,
made of a flexible material,
A space for accommodating the desiccant is provided inside.
that the overall shape is formed in a dome shape
A method for producing a cube-type natural Korean beef seasoning using roasted mushrooms comprising a.
제1항에 있어서,
(a) 원료준비단계는
(a1) 마늘분말을 65℃에서 1차 건조 후 이물 및 불순물을 제거하고 건조기에서 65℃에서 2차 건조 후 파쇄 및 분쇄하여 마늘분말을 제조하는 마늘분말 제조단계,
(a2) 표고버섯을 햇빛에서 1~2일간 1차 건조하고, 이물,불순물 선별한 후 건조기에 투입하여 65℃에서 2차 건조한 후 180~200℃의 온도에서 20~45분 간 로스팅을 실시하고, 파쇄 및 분쇄하여 로스팅표고버섯 분말을 제조하는 로스팅표고버섯 분말 제조단계, 및
(a3) 원료를 60℃의 온도에서 1차 건조시킨 후, 원료를 물에 끓여서 추출을 진행하고, 끓이고 잔여한 침전물을 여과 및 농축시킨 후 저온살균과정을 거치고, 건조기에 원료와 알파미분을 투입하여 진공건조시킨 후, 파쇄 및 분쇄하여 농축액분말을 제조하는 농축액분말 제조단계
를 포함하는 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법.
According to claim 1,
(a) The raw material preparation stage is
(a1) Garlic powder manufacturing step in which the garlic powder is first dried at 65° C., foreign substances and impurities are removed, and then crushed and pulverized after secondary drying at 65° C. in a dryer to produce garlic powder;
(a2) Dry the shiitake mushrooms in sunlight for 1-2 days, select foreign substances and impurities, put them in a dryer, dry them secondarily at 65℃, and roast them at 180-200℃ for 20 to 45 minutes. , A roasting shiitake mushroom powder manufacturing step of producing roasted shiitake mushroom powder by crushing and pulverizing, and
(a3) After drying the raw materials at a temperature of 60° C., boiling the raw materials in water to proceed with extraction, filtering and concentrating the remaining precipitate after boiling, and then pasteurizing the raw materials, and putting the raw materials and alpha fine powder in the dryer After vacuum drying, crushing and pulverizing to prepare a concentrate powder
A method for producing a cube-shaped natural Korean beef seasoning using roasted mushrooms comprising a.
제2항에 있어서,
(a1) 내지 (a3)단계는
파쇄 및 분쇄 이후, 쇠가루의 혼입으로 인한 금속성분을 검출하여 제거하는 분급 및 금속제거단계와, 수분활성도를 측정하여 일정 범위 초과인 경우 건조기에 투입하여 진공건조시키는 수분활성도 조절단계
를 더 포함하는 로스팅 버섯을 이용한 큐브형 천연 한우조미료 제조방법.

3. The method of claim 2,
Steps (a1) to (a3) are
After crushing and pulverization, a classification and metal removal step of detecting and removing metal components due to the mixing of iron powder, and a water activity control step of measuring the water activity and, if it exceeds a certain range, putting it into a dryer and vacuum drying
Cube-type natural Korean beef seasoning manufacturing method using roasted mushrooms further comprising a.

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