KR102346505B1 - Raw jujube mixed drink with antioxidant function and its manufacturing method - Google Patents
Raw jujube mixed drink with antioxidant function and its manufacturing method Download PDFInfo
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Images
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
- A23L2/04—Extraction of juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/72—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/302—Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
Abstract
Description
본 발명은 항산화능을 갖는 생대추를 이용한 혼합음료 및 이의 제조방법에 관한 것이다. The present invention relates to a mixed drink using raw jujube having antioxidant activity and a method for preparing the same.
대추(Zizyphus jujuba Milller)는 갈매나무과(Rhamnaceae)에 속하는 낙엽활엽교목의 열매로, 유럽 남부, 아시아 남부 및 동부가 원산지로 우리나라를 비롯한 중국, 일본에 분포하고 있으며 우리나라에서는 대추가 중국에서 전해진 것으로 보이지만 구체적인 기록은 없고, 본격적인 재배는 고려시대부터 시작되었을 것이라고 추정한다. 우리나라에서는 대추의 주산지의 명칭을 쳐서 대추 이름을 정해 왔으며, 재래종으로는 복조, 보은대추, 산조가 있고, 개량한 우량품종으로는 무등대추와 금성대추가 있다.Jujube ( Zizyphus jujuba Milller) is a fruit of a deciduous broad-leaved tree belonging to the Rhamnaceae family. There are no specific records, and it is assumed that full-scale cultivation began in the Goryeo Dynasty. In Korea, the names of jujubes have been determined by naming the main producing regions of jujubes, and conventional varieties include Bokjo, Boeun jujube, and Sanjo, and improved varieties include Mudung jujube and Geumseong jujube.
대추는 예로부터 건강식품으로서 영양성분이 풍부하며 한의약에는 감초와 함께 빠질 수 없는 재료이며, 대추의 성분으로는 당질과 아스코르브산(ascorbic acid)이 많으며 약용성분으로는 각종 스테롤(sterols), 알칼로이드(alkaloids), 사포닌(saponins), 비타민(vitamins), 유기산류, 아미노산류 등이 보고되고 있다. 그 효능으로는 완화제, 이뇨제, 강장제, 담즙증, 강정, 체력회복, 거담제, 항염증제 등의 약리효과 등이 보고·확인되어 한약제로 다양하게 사용된다. Jujube is rich in nutrients as a health food since ancient times, and is an essential ingredient along with licorice in herbal medicine. Jujube contains a lot of carbohydrates and ascorbic acid. alkaloids), saponins, vitamins, organic acids, and amino acids have been reported. As for its efficacy, pharmacological effects such as laxatives, diuretics, tonics, cholestasis, gangrene, stamina recovery, expectorants, and anti-inflammatory drugs have been reported and confirmed, and are used in various herbal medicines.
그러나 대추는 수확기간이 10일 정도로 짧으며, 저장성이 좋지 못하기 때문에, 생과 형태로는 수확시기에 극히 일부만 소비될 뿐 대부분 대추를 말린 형태로 유통 및 소비가 이루어지고 있다. 또한, 대부분의 말린 대추는 열풍 건조 방법을 이용하여 건조한 형태로 소비하게 되지만 열풍으로 건조할 경우 대추의 표면 수분이 빨리 말라서 대추의 표면이 급속도로 단단해지게 됨에 따라 상대적으로 내부 수분의 배출은 용이하지 않기 때문에, 색감이 어둡고 탁하게 나타나는 문제점이 있다. 특히 말린 대추는 차나 음료로 제조할 경우 색감이 어둡고 탁하게 나타나 시각적으로 좋지 않으며 대추에 포함된 영양소가 쉽게 파괴되는 문제점이 발생하게 된다. However, since the harvest period of jujube is short, about 10 days, and storage is not good, only a small part is consumed in the form of a raw fruit at the time of harvest, and most of it is distributed and consumed in the form of dried jujubes. In addition, most dried jujubes are consumed in a dry form using the hot air drying method, but when dried with hot air, the surface moisture of the jujube dries quickly and the surface of the jujube becomes hard rapidly, so it is relatively easy to discharge the internal moisture. Therefore, there is a problem that the color appears dark and cloudy. In particular, when dried jujubes are prepared as tea or beverages, the color is dark and cloudy, which is not visually good, and the nutrients contained in the jujubes are easily destroyed.
이에 본 발명자들은 생대추의 이용가치를 높이고 건조하지 않은 대추의 유용성분을 섭취하고자 생대추를 이용한 음료를 개발하였다. Accordingly, the present inventors developed a beverage using raw jujube to increase the use value of raw jujube and to consume useful components of non-dried jujube.
본 발명의 하나의 목적은 1) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 분쇄하여 갈아만든 생대추 주스를 제조하는 단계; 2) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 2 내지 4시간 가열하여 체에 내린 후 다시 가열하여 가열처리한 생대추 주스를 제조하는 단계; 및 3) 상기 가열처리한 생대추 주스와 상기 갈아만든 생대추 주스를 8:2 내지 9:1로 혼합하여 생대추 혼합음료를 제조하는 단계를 포함하는 생대추 혼합음료 제조방법을 제공한다.One object of the present invention is to prepare a raw jujube juice prepared by 1) separating the flesh and seeds of raw jujube, adding water to the separated raw jujube flesh and pulverizing it; 2) separating the flesh and seeds of the raw jujube, adding water to the separated raw jujube flesh, heating it for 2 to 4 hours, lowering it through a sieve, and heating again to prepare a heat-treated raw jujube juice; and 3) mixing the heat-treated raw jujube juice and the ground raw jujube juice in a ratio of 8:2 to 9:1 to prepare a raw jujube mixed drink.
본 발명의 다른 목적은 상기의 제조방법으로 제조된 생대추 혼합음료를 제공한다.Another object of the present invention is to provide a raw jujube mixed drink prepared by the above manufacturing method.
본 발명의 일 양상은 1) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 분쇄하여 갈아만든 생대추 주스를 제조하는 단계; 2) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 2 내지 4시간 가열하여 체에 내린 후 다시 가열하여 가열처리한 생대추 주스를 제조하는 단계; 및 3) 상기 가열처리한 생대추 주스와 상기 갈아만든 생대추 주스를 8:2 내지 9:1로 혼합하여 생대추 혼합음료를 제조하는 단계를 포함하는 생대추 혼합음료 제조방법을 제공한다.One aspect of the present invention comprises the steps of: 1) separating the flesh and seeds of raw jujube, adding water to the separated raw jujube flesh, pulverizing the raw jujube juice; 2) separating the flesh and seeds of the raw jujube, adding water to the separated raw jujube flesh, heating it for 2 to 4 hours, lowering it through a sieve, and heating again to prepare a heat-treated raw jujube juice; and 3) mixing the heat-treated raw jujube juice and the ground raw jujube juice in a ratio of 8:2 to 9:1 to prepare a raw jujube mixed drink.
본 발명의 일 구체예에 따르면, 상기 단계 1)의 갈아만든 생대추 주스는 -18℃ 내지 -20℃에서 냉동후 분쇄하는 단계를 추가적으로 수행하는 것일 수 있다.According to one embodiment of the present invention, the raw jujube juice ground in step 1) may be additionally frozen at -18°C to -20°C and then pulverized.
본 발명의 일 구체예에 따르면, 상기 단계 1)의 물 0.8L 부피를 기준으로, 상기 단계 1)의 생대추의 중량은 800g 내지 900g일 수 있다.According to one embodiment of the present invention, based on the 0.8L volume of water in step 1), the weight of the raw jujube in step 1) may be 800g to 900g.
본 발명의 일 구체예에 따르면, 상기 단계 2)의 물 4.5L 부피를 기준으로, 상기 단계 2)의 생대추의 중량은 800g 내지 900g일 수 있다.According to one embodiment of the present invention, based on the 4.5L volume of water in step 2), the weight of the raw jujube in step 2) may be 800g to 900g.
본 발명의 일 구체예에 따르면, 3) 단계 이후, 4) 갯방풍 나물, 세발나물 및 아이스플랜트로 이루어진 군으로부터 선택되는 하나 이상의 식물의 착즙액을 생대추 혼합음료와 혼합하는 단계를 더 포함할 수 있다.According to one embodiment of the present invention, after step 3), the method may further include the step of 4) mixing the juice of one or more plants selected from the group consisting of gaetbangpung herbs, tribal herbs and ice plants with a raw jujube mixed drink. have.
본 발명의 일 구체예에 따르면, 상기 생대추 혼합음료 및 상기 착즙액의 중량비는 20:1 내지 20:4일 수 있다.According to one embodiment of the present invention, the weight ratio of the raw jujube mixed drink and the juice liquid may be 20:1 to 20:4.
본 발명의 다른 양상은 상기 방법으로 제조된 생대추 혼합음료를 제공한다.Another aspect of the present invention provides a raw jujube mixed drink prepared by the above method.
본 발명의 갈아만든 생대추 주스는 전통적으로 제조된 건대추를 이용한 대추차보다 항산화 활성이 뛰어나고 프레쉬한 생대추의 맛을 살려내었으므로 기능적 및 관능적으로도 우수한 음료를 제공할 수 있다. The ground raw jujube juice of the present invention has superior antioxidant activity than the traditionally prepared jujube tea using dried jujube and preserves the fresh taste of raw jujube, so it can provide a functionally and sensually superior beverage.
도 1은 생대추 혼합음료의 제조공정을 나타낸 모식도이다.1 is a schematic diagram showing the manufacturing process of a raw jujube mixed drink.
이하, 본 발명의 실시예를 설명하기 위한 도 1을 참조하여 상세히 설명하기로 한다.Hereinafter, it will be described in detail with reference to FIG. 1 for explaining an embodiment of the present invention.
도 1은 본 발명의 실시예에 따른 항산화 기능성을 가지는 생대추 혼합음료의 제조공정을 순차적으로 도시한 모식도이다.1 is a schematic diagram sequentially illustrating a manufacturing process of a raw jujube mixed drink having antioxidant functionality according to an embodiment of the present invention.
본 발명의 실시예에 따른 생대추를 이용한 음료의 제조방법은 생대추의 과육과 씨를 분리하는 단계를 포함할 수 있다. 상기 생대추의 과육은 생대추의 껍질을 포함할 수 있고, 상기 분리하는 단계는 생대추를 껍질째 씨만 분리하여 제거하는 것을 의미할 수 있다. 대추의 씨는 단단하여 분쇄하기 어려우므로 분리하였다. 분리된 생대추의 과육의 일부는 갈아만든 생대추 주스 제조에 이용하고, 나머지 생대추 과육은 가열처리한 생대추 주스에 이용할 수 있다. The method of manufacturing a beverage using raw jujube according to an embodiment of the present invention may include separating the flesh and seeds of the raw jujube. The flesh of the raw jujube may include the skin of the raw jujube, and the separating may mean removing only the peeled seeds of the raw jujube. Jujube seeds were separated because they were hard and difficult to grind. A part of the separated raw jujube pulp can be used for manufacturing raw jujube juice, and the remaining raw jujube pulp can be used for heat-treated raw jujube juice.
분리된 생대추의 과육에 물을 첨가하여 분쇄하여 갈아만든 생대추 주스를 제조하였다. 구체적으로, 생대추 60개(840g)에 물 0.8L를 넣고 분쇄기로 분쇄하여 1.65L의 갈아만든 생대추 주스를 제조하였으며, 당도는 16.0brix 였다. 생대추 단독에 대한 당도를 측정하였을 때는 26.5brix였다. 분쇄한 생대추를 -18℃ 내지 -20℃에서 냉동후 파코젯을 이용하여 추가로 분쇄할 수 있다. 생대추의 껍질은 일반 분쇄기로 분쇄할 경우 입자가 완전히 갈리지 않아서 파코젯(Pacojet; 극세분쇄요리기)을 이용하여 추가로 분쇄하여 생대추의 껍질을 완전히 분쇄할 수 있다.Water was added to the separated raw jujube flesh and pulverized to prepare raw jujube juice. Specifically, 0.8L of water was added to 60 raw jujubes (840g) and ground with a grinder to prepare 1.65L of ground raw jujube juice, and the sugar content was 16.0brix. When the sugar content of raw jujube alone was measured, it was 26.5brix. After the crushed raw jujube is frozen at -18°C to -20°C, it can be further crushed using a Pacojet. When the raw jujube skin is pulverized with a general grinder, the particles are not completely ground, so the skin of the raw jujube can be completely pulverized by additionally pulverizing it using a Pacojet (fine grinding cooker).
분리된 생대추의 과육에 물을 첨가하여 2 내지 4시간 가열하여 체에 내린 후 다시 가열하여 가열처리한 생대추 주스를 제조하였다. Water was added to the separated raw jujube flesh, heated for 2 to 4 hours, lowered through a sieve, and heated again to prepare heat-treated raw jujube juice.
구체적으로, 생대추의 가열시간은 2 내지 4시간이 바람직하며 보다 바람직하게는 3시간일 수 있다. 생대추 60개에 물 4.5L를 넣고 약불에서 약 3시간 끓인 후 체에 내린 후 껍질을 거른 다음 다시 끓여주어 1.65L의 대추 추출물을 제조하였고, 가열한 생대추를 이용한 음료의 당도는 10brix였다.Specifically, the heating time of the raw jujube is preferably 2 to 4 hours, and more preferably 3 hours. Add 4.5L of water to 60 raw jujubes, boil over low heat for about 3 hours, remove the skin through a sieve, and then boil again to prepare 1.65L of jujube extract.
가열처리한 생대추 주스과 갈아만든 생대추 주스를 8:2 내지 9:1의 중량비로 혼합하여 갈아만든 생대추 주스를 제조하였다. 가열처리한 생대추 주스과 갈아만든 생대추 주스를 8:2 내지 9:1로 혼합하는 것이 바람직하고 보다 바람직하게는 8:2로 혼합하는 것이 바람직하다.The heat-treated raw jujube juice and the ground raw jujube juice were mixed in a weight ratio of 8:2 to 9:1 to prepare a ground raw jujube juice. It is preferable to mix the heat-treated raw jujube juice and the ground raw jujube juice in a ratio of 8:2 to 9:1, more preferably 8:2.
본 발명에 있어서, 가열처리한 생대추 주스과 갈아만든 생대추 주스는 항산화능, 당도, 관능적 특성이 다른점이 있으므로 생대추가 가지는 프레쉬한 느낌은 최대한 살리면서 항산화능이 뛰어난 갈아만든 생대추 주스를 제조하기 위하여 갈아만든 생대추 주스와 가열처리한 생대추 주스를 혼합하였다. In the present invention, since the heat-treated raw jujube juice and the ground raw jujube juice differ in antioxidant activity, sugar content, and sensory properties, the raw jujube grinded to produce the raw jujube juice with excellent antioxidant activity while maximizing the fresh feeling of the raw jujube The juice and the heat-treated raw jujube juice were mixed.
따라서, 본 발명은 상기의 방법으로 제조된 생대추 혼합음료 제공한다. Accordingly, the present invention provides a raw jujube mixed drink prepared by the above method.
한편, 본 발명의 일 실시예에 따라 제조된 생대추 혼합음료의 총 페놀 및 총 플라보노이드 함량 및 항산화능을 개선하고, 관능성을 증진시키기 위하여 갯방풍 나물, 세발나물 및 아이스플랜트로 이루어진 군으로부터 선택되는 하나 이상의 식물 착즙액을 더 혼합할 수 있다.On the other hand, in order to improve the total phenol and total flavonoid content and antioxidant capacity of the raw jujube mixed drink prepared according to an embodiment of the present invention, and to enhance sensory properties, it is selected from the group consisting of gaetbangpungnamul, sebalnamul, and ice plant. One or more plant juices may be further mixed.
갯방풍 나물, 세발나물 또는 아이스플랜트의 착즙액이 더 혼합된 생대추 혼합음료는 상기 착즙액이 혼합되지 않은 생대추 혼합음료보다 특히 청량감, 향 및 종합적인 기호도가 현저히 우수하며, 이는 상기 착즙액의 은은하면서도 상쾌한 향이 생대추의 풀향을 잡아주고, 단조로운 생대추 혼합음료의 단맛에 착즙액의 상큼함이 더해져 청량한 느낌이 배가되기 때문이다.The raw jujube mixed drink in which the juice of Gaetbangpung herbs, tribal herbs, or ice plant is further mixed is particularly superior in refreshing feeling, flavor, and overall preference compared to the raw jujube mixed drink in which the juice is not mixed. This is because the fresh yet refreshing scent captures the grassy scent of raw jujube, and the freshness of the juice is added to the sweetness of the monotonous raw jujube mixed drink, which doubles the refreshing feeling.
생대추 혼합음료와 상기 착즙액이 20:1 미만의 중량비로 혼합될 경우, 착즙액으로부터 기인하는 청량감 및 향이 부족하여 생대추 혼합음료의 관능성에 기여하는 바가 미미하고, 20:4 초과의 중량비로 혼합될 경우, 착즙액의 쌉싸름한 맛이 지나치게 강해져 생대추 혼합음료의 종합적인 기호도가 반감된다.When the raw jujube mixed drink and the juice are mixed in a weight ratio of less than 20: 1, the freshness and flavor resulting from the juice is insufficient, contributing to the sensuality of the raw jujube mixed beverage is insignificant, and it is to be mixed at a weight ratio of more than 20:4. In this case, the bitter taste of the juice is too strong and the overall preference of the raw jujube mixed drink is halved.
따라서, 생대추 혼합음료와 상기 착즙액은 20:1 내지 20:4의 중량비로 혼합되는 것일 수 있고, 바람직하게는 20:2의 중량비로 혼합된 것일 수 있다.Therefore, the raw jujube mixed beverage and the juice may be mixed in a weight ratio of 20: 1 to 20: 4, and preferably mixed in a weight ratio of 20: 2.
이하 본 발명을 하나 이상의 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 본 발명을 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail through one or more embodiments. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited to these examples.
<실시예 1> 대추를 이용 음료의 제조방법<Example 1> Manufacturing method of beverage using jujube
<1-1> 생대추를 이용한 음료의 제조방법 <1-1> Manufacturing method of beverage using raw jujube
생대추(충남 보은군) 60개(840g)의 과육과 씨를 분리한 다음 과육에 물 0.8L를 넣고 분쇄기로 분쇄하여 1.65L의 생대추를 이용한 음료를 제조하였다. 생대추를 이용한 음료의 당도는 16.0brix였다. After separating the flesh and seeds of 60 raw jujubes (Boeun-gun, Chungcheongnam-do), 0.8L of water was added to the pulp and crushed with a grinder to prepare a beverage using 1.65L of raw jujubes. The sugar content of the drink using raw jujube was 16.0brix.
<1-2> 가열한 생대추를 이용한 음료의 제조방법<1-2> Manufacturing method of beverage using heated raw jujube
생대추(충남 보은군) 60개(840g)의 과육과 씨를 분리한 다음 과육에 물 4.5L를 넣고 약불에서 약 3시간 끓인 후 체에 내린 후 껍질을 거른 다음 다시 끓여주어 1.65L의 가열한 생대추를 이용한 음료를 제조하였다. 가열한 생대추를 이용한 음료의 당도는 10brix였다.After separating the flesh and seeds of 60 raw jujubes (Boeun-gun, Chungcheongnam-do), add 4.5L of water to the pulp, boil it for about 3 hours over low heat, remove it through a sieve, filter the skin, and boil again using 1.65L of heated raw jujube. Beverages were prepared. The sugar content of the beverage using heated raw jujube was 10brix.
<비교예> 건대추를 이용한 전통차의 제조방법<Comparative example> Manufacturing method of traditional tea using dried jujube
건대추(충남 보은군) 60개(600g)에 물 4.0L를 넣고 약불에서 약 3시간 끓인 후 체에 받쳐 씨와 껍질을 거른 후 다시 끓여서 1.65L의 건대추를 이용한 전통차를 제조하였다. 건대추를 이용한 전통차의 당도는 10.8brix였다.Add 4.0L of water to 60 dried jujubes (600g) in Boeun-gun, Chungcheongnam-do, boil for about 3 hours on low heat, strain through a sieve, filter seeds and skins, and boil again to prepare a traditional tea using 1.65L dried jujubes. The sugar content of the traditional tea using dried jujube was 10.8 brix.
<실시예 2> 생대추를 이용한 음료의 항산화능 분석<Example 2> Analysis of antioxidant activity of beverages using raw jujube
상기 실시예 1에서 제조한 생대추를 이용한 음료와 건대추를 이용한 전통차의 항산화능을 분석하기 위하여 시료를 제조하였다. 상기 실시예 1에서 제조한 생대추를 이용한 음료와 비교예에서 제조한 건대추를 이용한 전통차를 동결건조기(MCFD 8505, Ilshin Lab Co., Seoul, Korea)를 이용하여 -80℃에서 96시간 건조 후, 분쇄기(HB-310, Hibell Co., Seoul, Korea)로 분쇄하여 100mesh 정밀망에 내려 분말화하였다. 제조한 각 분말 50g에 60% 에틸 알코올(ethyl alcohol) 1000mL를 넣은 후, 24시간 동안 25℃에서 교반 추출하여 상등액을 분리 후 여과지(Whatman No. 4)로 여과하였다. 여과 후, rotary evaporator(EYELA Co, 7N-1000, Tokyo, Japan)로 농축하고, -70℃의 초저온 냉장고(deep freezer, New Brunswik Scientific Co. New Brunswick, USA)에서 24시간 동결시킨 후 72시간 동안 동결건조(Freezer Dryer, Operon Eng Co., Seoul, Korea)하였다. 동결 건조된 시료를 막사발을 이용하여 분쇄한 후 100 mesh 표준 망체를 통과시켜 분말화한 후 -4℃에서 보관하여 사용하였다.A sample was prepared to analyze the antioxidant activity of the beverage using the raw jujube prepared in Example 1 and the traditional tea using the dried jujube. After drying the beverage using the raw jujube prepared in Example 1 and the traditional tea using the dried jujube prepared in Comparative Example at -80°C for 96 hours using a freeze dryer (MCFD 8505, Ilshin Lab Co., Seoul, Korea), It was pulverized with a grinder (HB-310, Hibell Co., Seoul, Korea) and put down on a 100mesh precision mesh to be powdered. After adding 1000 mL of 60% ethyl alcohol to 50 g of each powder prepared, extraction was performed with stirring at 25° C. for 24 hours, and the supernatant was separated and filtered through filter paper (Whatman No. 4). After filtration, it was concentrated with a rotary evaporator (EYELA Co, 7N-1000, Tokyo, Japan), and frozen in a deep freezer at -70 ° C (deep freezer, New Brunswik Scientific Co. New Brunswick, USA) for 24 hours and then for 72 hours It was freeze-dried (Freezer Dryer, Operon Eng Co., Seoul, Korea). After the freeze-dried sample was pulverized using a barnacle, it was powdered by passing it through a 100 mesh standard mesh sieve, and then stored at -4°C for use.
<2-1> 총 페놀 분석<2-1> Total phenol analysis
상기 실시예 1에서 제조한 생대추를 이용한 음료와 건대추를 이용한 전통차에 포함된 폴리페놀의 양을 측정하였다.The amount of polyphenols contained in the beverage using the raw jujube prepared in Example 1 and the traditional tea using the dried jujube was measured.
구체적으로, 총 폴리페놀 함량은 Folin-Ciocalteu's 방법(Amerine & Ough 1980)에 따라 시료 150 ㎕, 증류수 2.4 mL, 2 N Folin-Ciocalteu reagent(Sigma-Aldrich Co, St Louis, Mo, USA) 150 ㎕, 20% Na2CO3 300 ㎕를 혼합하여 암소에서 1시간 반응시킨 후 750 nm에서 분광광도계를 통해 흡광도 값을 측정하였다. 페놀화합물 함량은 표준물질인 갈산(gallic acid, Sigma-Aldrich Co)를 사용하여 상기의 방법으로 작성한 표준곡선으로 양을 환산하였고, 추출물 중의 GAE(mg gallic acid equivalent) mg/g으로 나타내었다.Specifically, the total polyphenol content was 150 μl of a sample according to Folin-Ciocalteu's method (Amerine & Ough 1980), 2.4 mL of distilled water, 150 μl of 2 N Folin-Ciocalteu reagent (Sigma-Aldrich Co, St Louis, Mo, USA); After mixing 300 μl of 20% Na 2 CO 3 and reacting in the dark for 1 hour, the absorbance value was measured at 750 nm through a spectrophotometer. The phenolic compound content was converted to the standard curve prepared by the above method using gallic acid (Sigma-Aldrich Co), a standard material, and expressed as mg/g of GAE (mg gallic acid equivalent) in the extract.
그 결과, 생대추를 이용한 음료가 건대추를 이용한 전통차보다 총페놀 함량이 높았으며, 가열처리한 생대추 주스가 갈아만든 생대추 주스보다 총페놀 함량이 높은 것을 확인하였다(표 1). As a result, it was confirmed that the beverage using raw jujube had a higher total phenol content than traditional tea using dried jujube, and that the heat-treated raw jujube juice had a higher total phenol content than the ground raw jujube juice (Table 1).
(mg GAE/g)Total phenol content
(mg GAE/g)
GAE:Galic acid, All values represent Mean ± S.D. of triplicate independent experimentsGAE:Galic acid, All values represent Mean ± S.D. of triplicate independent experiments
<2-2> 총 플라보노이드 분석<2-2> Total flavonoid analysis
상기 실시예 1에서 제조한 생대추를 이용한 음료와 건대추를 이용한 전통차에 포함된 폴리페놀 중에서도 강한 항산화력을 갖는 것으로 알려진 플라보노이드의 양을 측정하였다.The amount of flavonoids known to have strong antioxidant power among polyphenols contained in beverages using raw jujube and traditional tea using dried jujubes prepared in Example 1 was measured.
총 플라보노이드 함량은 Moreno 등의 방법을 이용하여 비색 정량하였다. 생대추를 이용한 음료 1 mL에 90% diethylene glycol 10 mL, 1 N NaOH 1 mL를 첨가하여 혼합한 후 37℃ 항온수조에서 시간 반응시키고 UV-Spectrophotometer(Molecular devices, SpectraMax M2, Sunnyvale, CA, USA)를 사용하여 420 nm 에서 흡광도를 측정하였다. 총 플라보노이드 함량은 퀘르세틴(quercetin, Sigma-Aldrich, St. Louis, MO, USA)을 표준물질로 하여 검량선을 작성하였고 플라보노이드 함량은 건조 시료 중량 당 QE(quercetin equivalent) mg/g로 나타내었다.Total flavonoid content was colorimetrically quantified using the method of Moreno et al. After adding and mixing 10 mL of 90% diethylene glycol and 1 mL of 1 N NaOH to 1 mL of a beverage using raw jujube, time-reacted in a constant temperature water bath at 37°C, followed by a UV-Spectrophotometer (Molecular devices, SpectraMax M2, Sunnyvale, CA, USA). was used to measure the absorbance at 420 nm. The total flavonoid content was calculated using quercetin (quercetin, Sigma-Aldrich, St. Louis, MO, USA) as a standard material, and the flavonoid content was expressed as QE (quercetin equivalent) mg/g per dry sample weight.
그 결과, 생대추를 이용한 음료가 건대추를 이용한 전통차보다 총플라보노이드 함량이 월등히 높았으며, 가열처리한 생대추 주스가 갈아만든 생대추 주스보다 총플라보노이드 함량이 높은 것을 확인하였다(표 2). As a result, it was confirmed that the beverage using raw jujube had a significantly higher total flavonoid content than the traditional tea using dried jujube, and that the heat-treated raw jujube juice had a higher total flavonoid content than the ground raw jujube juice (Table 2).
함량
(mg QE/g)total flavonoids
content
(mg QE/g)
QE:Quercertin, All values represent Mean ± S.D. of triplicate independent experimentsQE: Quercertin, All values represent Mean ± S.D. of triplicate independent experiments
<2-3> 라디컬 소거능 분석<2-3> Radical scavenging ability analysis
활성산소(DPPH radical) 소거 효능을 측정하는 것으로 활성산소를 포함하고 있는 시약인 DPPH가 항산화 물질에 의해 활성산소가 제거되면 색이 변하는 것을 이용하여 활성산소가 제거되는 정도를 색농도로 측정한다.To measure the effectiveness of DPPH radical scavenging, the color concentration of DPPH, a reagent containing active oxygen, changes color when active oxygen is removed by an antioxidant.
구체적으로, 80 ㎕의 0.2mM DPPH 에탄올 용액에 생대추를 이용한 음료, 건대추를 이용한 전통차 20 ㎕를 가한 후 10초 동안 잘 혼합해 주고 상온에서 10분간 반응시켜 ELISA (Shimadzu UV-1601, Tokyo, Japan)을 사용하여 517nm에서 흡광도를 측정하였다. 무처리구에는 20㎕의 DMSO로 처리하여 흡광도를 측정하였다. 각 시료의 DPPH radical 소거능은 다음의 식에 의해 계산하였다.Specifically, after adding 20 μl of a drink using raw jujube and a traditional tea using dried jujube to 80 μl of 0.2 mM DPPH ethanol solution, mix well for 10 seconds and react at room temperature for 10 minutes to perform ELISA (Shimadzu UV-1601, Tokyo, Japan) ) was used to measure the absorbance at 517 nm. The untreated group was treated with 20 μl of DMSO and absorbance was measured. The DPPH radical scavenging ability of each sample was calculated by the following formula.
Radical scavenging activity (RSA,%) = (1-A/B)Radical scavenging activity (RSA,%) = (1-A/B)
A: 시료 첨가구의 흡광도, B: 무처리구의 흡광도A: absorbance of the sample addition section, B: absorbance of the untreated section
그 결과, 생대추를 이용한 음료가 건대추를 이용한 전통차보다 활성 산소 소거능력이 높았으며, 가열처리한 생대추 주스가 갈아만든 생대추 주스보다 활성 산소 소거능력이 높았다. 특히 갈아만든 생대추 주스는 대조군(BTH) 보다도 더 높은 활성 산소 소거능력이 있음을 확인하였다(표 3). As a result, the beverage using raw jujube had higher active oxygen scavenging ability than traditional tea using dried jujube, and the heat-treated raw jujube juice had a higher active oxygen scavenging ability than the ground raw jujube juice. In particular, it was confirmed that the ground raw jujube juice had a higher active oxygen scavenging ability than the control (BTH) (Table 3).
(1mg/ml)DPPH radical scavenging activity (%)
(1mg/ml)
(0.000)*** 154.194
(0.000) ***
All values are Mean ± S.D. of three independent experriment. (p<0.005)All values are Mean ± S.D. of three independent experiments. (p<0.005)
<실시예 3> 생대추를 이용한 음료의 색도 분석<Example 3> Analysis of chromaticity of beverages using raw jujube
상기 실시예 1에서 제조한 생대추를 이용한 주스와 가열처리한 건대추 전통차의 색도를 분석하였다. The chromaticity of the juice using the raw jujube prepared in Example 1 and the heat-treated dried jujube traditional tea was analyzed.
구체적으로, 색도계(Chroma Meter CR-300, Minolta Co., Osaka, Japan)를 이용하여 명도(Lightness, L), 적색도(Redness, a), 황색도(Yellowness, b) 및 총색차(E=√(L₁-L₂)²+(a₁-a₂)²=(b₁-b₂)²)의 값을 3회 반복하여 측정하였으며, 그 평균값을 구하였다. 이때 사용한 표준 백판(Standard white plate)의 L 값은 93.35, a 값은 -0.67, b 값은 +4.67이다.Specifically, using a colorimeter (Chroma Meter CR-300, Minolta Co., Osaka, Japan), brightness (L), redness (Redness, a), yellowness (Yellowness, b) and total color difference (E= The value of √(L₁-L₂)²+(a₁-a₂)²=(b₁-b₂)²) was measured three times, and the average value was obtained. At this time, the L value of the standard white plate used was 93.35, the a value was -0.67, and the b value was +4.67.
통계처리는 SPSS(Statistics Package for Social Science, Ver.18.0) 프로그램을 이용하여 실시하였다. 각 시료간의 평균 차이를 보기 위하여 일원분산분석(One-way analysis of variance)를 실시하였고, 유의적인 차이가 있을 경우 p<0.05 수준에서 Duncan의 다중범위 시험법(Duncan‘s multiple range test)으로 사후검정을 실시하였다. Statistical processing was performed using SPSS (Statistics Package for Social Science, Ver.18.0) program. One-way analysis of variance was performed to see the mean difference between each sample. The assay was carried out.
그 결과, 갈아만든 생대추 주스나 가열처리한 생대추 주스에 비하여 가열처리한 건대추 전통차가 명도 값(L)이 낮은 것으로 어두운 것을 확인하였다(표 4).As a result, it was confirmed that the heat-treated dried jujube traditional tea had a lower brightness value (L) than that of ground raw jujube juice or heat-treated raw jujube juice (Table 4).
<실시예 4> 생대추 음료의 제조방법 및 관능평가<Example 4> Manufacturing method and sensory evaluation of raw jujube beverage
<4-1> 생대추 음료의 제조방법<4-1> Manufacturing method of raw jujube drink
상기 실시예 1에서 제조한 갈아만든 생대추 주스와 가열처리한 생대추 주스는 가열처리한 건대추 전통차보다 항산화능이 우수하고 색도도 밝았다. 가열처리한 생대추 주스는 생대추 주스와 비교하여 항산화능이 조금 더 우수하였지만 생대추가 가지는 프레쉬함이 없으므로 생대추를 이용한 두가지 주스를 하기 표 5에 기재한 바와 같이 다양한 비율로 혼합하여 생대추 음료를 제조하였다. The ground raw jujube juice prepared in Example 1 and the heat-treated raw jujube juice had better antioxidant activity and brighter color than the heat-treated dried jujube traditional tea. The heat-treated raw jujube juice had slightly better antioxidant activity compared to the raw jujube juice, but since the raw jujube had no freshness, two juices using the raw jujube were mixed in various proportions as shown in Table 5 below to prepare a raw jujube drink.
<4-2> 생대추 음료의 관능평가<4-2> Sensory evaluation of raw jujube drinks
생대추 음료에 대한 관능평가는 scoring test로 진행되었으며, 사전에 관능평가에 대한 교육 받은 15명의 숙련된 식품영양학과 대학원생을 패널로 선정하였다. 선정된 패널에게 4개의 시료가 무작위로 제공되었으며 패널들은 색, 단맛, 향, 종합적 기호도를 평가하도록 안내받았다. 이때 각각의 항목에 대한 7점 척도법(1점 매우 나쁘다, 7점, 매우 좋다)으로 평가하도록 하였다. 하나의 시료를 평가한 후 물로 입안을 헹구도록하고 다른 시료를 시식하도록하였다. 관능평가지를 작성할 수 있는 충분한 공간이 제공되었으며 평가시간은 10분 내외로 제한하였다.The sensory evaluation of the raw jujube drink was conducted through a scoring test, and 15 experienced graduate students of the Department of Food and Nutrition, who had been educated on the sensory evaluation in advance, were selected as a panel. Four samples were randomly provided to the selected panel, and the panelists were instructed to evaluate color, sweetness, aroma, and overall preference. At this time, each item was evaluated on a 7-point scale (1 point very bad, 7 points, very good). After evaluating one sample, the mouth was rinsed with water and the other sample was sampled. Sufficient space was provided to prepare the sensory evaluation sheet, and the evaluation time was limited to around 10 minutes.
그 결과, 갈아만든 생대추 주스와 가열처리한 생대추 주스의 혼합 비율이 20:80이였을때 색, 단맛, 향 및 종합적 기호도가 높은 것을 확인하였다(표 6). As a result, it was confirmed that the color, sweetness, flavor, and overall preference were high when the mixing ratio of the ground raw jujube juice and the heat-treated raw jujube juice was 20:80 (Table 6).
(overall quality)Comprehensive symbol map
(overall quality)
<실시예 5> 생대추 혼합 음료의 제조 및 항산화 분석<Example 5> Preparation of raw jujube mixed beverage and analysis of antioxidants
<5-1> 생대추 혼합 음료의 제조방법<5-1> Manufacturing method of raw jujube mixed drink
상기 실시예 4에서 제조한 제조예 2의 생대추 음료의 기능성 및 기호도를 높이기 위하여 제조예 2의 생대추 음료에 세발나물(국산), 갯방풍나물(국산) 또는 아이스플란트(국산) 착즙액을 각각 10%(w/w)를 혼합하여 항산화능 및 관능평가를 실시하였다. 착즙은 착즙기(HZS-SBF19, 휴롬, 한국)를 이용하여 수행하였다. In order to increase the functionality and preference of the raw jujube beverage of Preparation Example 2 prepared in Example 4, the raw jujube drink of Preparation Example 2 was added with the extract of sebal namul (domestic), Gaetbang pungnamul (domestic) or ice plant (domestic), respectively. By mixing 10% (w/w), antioxidant activity and sensory evaluation were performed. Juicing was performed using a juicer (HZS-SBF19, Hurom, Korea).
<5-2><5-2> 총 페놀 분석Total phenol analysis
제조예 2의 생대추 음료에 세발나물(제조예 5), 갯방풍나물(제조예 6), 아이스플란트(제조예 7) 착즙액을 각각 10%(w/w)를 혼합한 생대추 혼합음료 각각에 포함된 폴리페놀의 양을 측정하였다.Each raw jujube mixed drink in which 10% (w/w) of the juice of the raw jujube drink of Preparation Example 2 was mixed with each of three balsamul (Preparation Example 5), Gaetbang Pungnamul (Preparation Example 6), and ice plant (Preparation Example 7). The amount of polyphenol contained in the was measured.
구체적으로, 총 폴리페놀 함량은 Folin-Ciocalteu's 방법(Amerine & Ough 1980)에 따라 시료 150 ㎕, 증류수 2.4 mL, 2 N Folin-Ciocalteu reagent(Sigma-Aldrich Co, St Louis, Mo, USA) 150 ㎕, 20% Na2CO3 300 ㎕를 혼합하여 암소에서 1시간 반응시킨 후 750 nm에서 분광광도계를 통해 흡광도 값을 측정하였다. 페놀화합물 함량은 표준물질인 갈산(gallic acid, Sigma-Aldrich Co)를 사용하여 상기의 방법으로 작성한 표준곡선으로 양을 환산하였고, 추출물 중의 GAE(mg gallic acid equivalent) μg/μg으로 나타내었다.Specifically, the total polyphenol content was 150 μl of a sample according to Folin-Ciocalteu's method (Amerine & Ough 1980), 2.4 mL of distilled water, 150 μl of 2 N Folin-Ciocalteu reagent (Sigma-Aldrich Co, St Louis, Mo, USA); After mixing 300 μl of 20% Na 2 CO 3 and reacting in the dark for 1 hour, the absorbance value was measured at 750 nm through a spectrophotometer. The phenolic compound content was converted to the standard curve prepared by the above method using gallic acid (Sigma-Aldrich Co), a standard material, and expressed as mg gallic acid equivalent (GAE) μg/μg in the extract.
그 결과, 세발나물 착즙액이 첨가된 생대추 혼합음료의 경우 총폴리페놀 함량이 감소한 반면, 갯방풍나물 착즙액 또는 아이스플란트 착즙액이 첨가된 생대추 혼합음료의 총폴리페놀 함량이 생대추 주스보다 높은 것을 확인하였다(표 7).As a result, the total polyphenol content was decreased in the case of the raw jujube mixed drink to which the extract of sebaceous plants was added, while the total polyphenol content of the raw jujube mixed drink added with the gaetbang pungnamul extract or ice plant extract was higher than that of the raw jujube juice. was confirmed (Table 7).
(GAE μg/μg)Total phenol content
(GAE μg/μg)
GAE:Galic acid, All values represent Mean ± S.D. of triplicate independent experimentsGAE:Galic acid, All values represent Mean ± S.D. of triplicate independent experiments
<5-3> 총 플라보노이드 분석<5-3> Total flavonoid analysis
제조예 2의 생대추 음료에 세발나물(제조예 5), 갯방풍 나물(제조예 6), 아이스플란트(제조예 7) 착즙액 각각 10%를 혼합한 생대추 혼합음료 각각에 포함된 폴리페놀 중에서도 강한 항산화력을 갖는 것으로 알려진 플라보노이드의 양을 측정하였다.Among the polyphenols contained in each of the raw jujube mixed drinks in which 10% of each extract was mixed with the raw jujube drink of Preparation Example 2 (Preparation Example 5), Gaetbangpung namul (Preparation Example 6), and ice plant (Preparation Example 7) The amount of flavonoids known to have strong antioxidant activity was measured.
총 플라보노이드 함량은 Moreno 등의 방법을 이용하여 비색 정량하였다. 생대추를 이용한 음료 1 mL에 90% diethylene glycol 10 mL, 1 N NaOH 1 mL를 첨가하여 혼합한 후 37℃ 항온수조에서 시간 반응시키고 UV-Spectrophotometer(Molecular devices, SpectraMax M2, Sunnyvale, CA, USA)를 사용하여 420 nm 에서 흡광도를 측정하였다. 총 플라보노이드 함량은 루틴(Rutin, Sigma-Aldrich, St. Louis, MO, USA)을 표준물질로 하여 검량선을 작성하였고 플라보노이드 함량은 건조 시료 중량 당 Rutin μg/μg로 나타내었다.Total flavonoid content was colorimetrically quantified using the method of Moreno et al. After adding and mixing 10 mL of 90% diethylene glycol and 1 mL of 1 N NaOH to 1 mL of a beverage using raw jujube, time-reacted in a constant temperature water bath at 37°C, followed by a UV-Spectrophotometer (Molecular devices, SpectraMax M2, Sunnyvale, CA, USA). was used to measure the absorbance at 420 nm. A calibration curve was prepared using rutin (Rutin, Sigma-Aldrich, St. Louis, MO, USA) as a standard for total flavonoid content, and flavonoid content was expressed as Rutin μg/μg per dry sample weight.
그 결과, 제조예 2 대비 갯방풍 나물, 세발나물 또는 아이스플란트 착즙액이 첨가된 생대추 혼합음료의 총플라보노이드 함량이 증가한 것을 확인하였으며, 특히 갯방풍 나물 착즙액이 첨가된 생대추 혼합음료의 총플라보노이드 함량이 현저히 증가한 것을 확인하였다(표 8).As a result, compared to Preparation Example 2, it was confirmed that the total flavonoid content of the raw jujube mixed drink added with Gaetbangpung herbs, Tribal herb or ice plant juice was increased. It was confirmed that the content was significantly increased (Table 8).
(Rutin μg/μg)Total flavonoid content
(Rutin μg/μg)
All values represent Mean ± S.D. of triplicate independent experimentsAll values represent Mean ± S.D. of triplicate independent experiments
<실시예 6> 생대추 혼합음료의 관능평가<Example 6> Sensory evaluation of raw jujube mixed drink
생대추 혼합 음료에 대한 관능평가는 scoring test로 진행되었으며, 사전에 관능평가에 대한 교육 받은 10명의 숙련된 식품영양학과 대학원생을 패널로 선정하였다. 선정된 패널에게 4개의 시료가 무작위로 제공되었으며 패널들은 색, 단맛, 향, 종합적 기호도를 평가하도록 안내받았다. 이때 각각의 항목에 대한 7점 척도법(1점 매우 나쁘다, 7점, 매우 좋다)으로 평가하도록 하였다. 하나의 시료를 평가한 후 물로 입안을 헹구도록하고 다른 시료를 시식하도록하였다. 관능평가지를 작성할 수 있는 충분한 공간이 제공되었으며 평가시간은 10분 내외로 제한하였다.The sensory evaluation of the raw jujube mixed drink was conducted as a scoring test, and 10 experienced graduate students of the Department of Food and Nutrition, who had been educated on the sensory evaluation in advance, were selected as a panel. Four samples were randomly provided to the selected panel, and the panelists were instructed to evaluate color, sweetness, aroma, and overall preference. At this time, each item was evaluated on a 7-point scale (1 point very bad, 7 points, very good). After evaluating one sample, the mouth was rinsed with water and the other sample was sampled. Sufficient space was provided to prepare the sensory evaluation sheet, and the evaluation time was limited to around 10 minutes.
그 결과, 갯방풍 나물, 세발나물, 아이스플랜트를 첨가한 생대추 혼합음료가 생대추 음료보다 관능성이 우수한 것을 확인하였으며, 그 중에서도 갯방풍 나물을 함유한 대추 혼합음료(제조예 6)의 관능성이 가장 우수한 것을 확인하였다.As a result, it was confirmed that the raw jujube mixed drink containing Gaetbangpung herbs, tribal herbs, and ice plant had better sensory properties than the raw jujube drink. The best was confirmed.
이제까지 본 발명에 대하여 그 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.So far, the present invention has been looked at focusing on the embodiments thereof. Those of ordinary skill in the art to which the present invention pertains will understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. The scope of the present invention is indicated in the claims rather than the foregoing description, and all differences within the scope equivalent thereto should be construed as being included in the present invention.
Claims (7)
1) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 분쇄하여 갈아만든 생대추 주스를 제조하는 단계;
2) 생대추의 과육과 씨를 분리하고, 분리된 생대추의 과육에 물을 첨가하여 2 내지 4시간 가열하여 체에 내린 후 다시 가열하여 가열처리한 생대추 주스를 제조하는 단계;
3) 상기 가열처리한 생대추 주스와 상기 갈아만든 생대추 주스를 8:2의 중량비로 혼합하여 생대추 혼합음료를 제조하는 단계; 및
4) 갯방풍 나물, 세발나물 및 아이스플랜트로 이루어진 군으로부터 선택되는 하나 이상의 식물의 착즙액을 생대추 혼합음료와 혼합하는 단계를 더 포함하고,
상기 단계 1)의 물 0.8L 부피를 기준으로, 상기 단계 1)의 생대추의 중량은 800g 내지 900g인 것이고,
상기 단계 2)의 물 4.5L 부피를 기준으로, 상기 단계 2)의 생대추의 중량은 800g 내지 900g인 것이고,
상기 4) 단계의 생대추 혼합음료 및 식물의 착즙액의 중량비는 20:2인 것인
생대추 혼합음료 제조방법.
In the raw jujube mixed drink manufacturing method,
1) preparing raw jujube juice by separating the flesh and seeds of raw jujube, adding water to the separated raw jujube flesh and pulverizing it;
2) separating the flesh and seeds of the raw jujube, adding water to the separated raw jujube flesh, heating it for 2 to 4 hours, lowering it through a sieve, and heating again to prepare a heat-treated raw jujube juice;
3) preparing a raw jujube mixed drink by mixing the heat-treated raw jujube juice and the ground raw jujube juice in a weight ratio of 8:2; and
4) further comprising the step of mixing the juice of one or more plants selected from the group consisting of Gaetbangpung herbs, tribal herbs, and ice plants with a raw jujube mixed drink,
Based on the 0.8L volume of water in step 1), the weight of the raw jujube in step 1) is 800g to 900g,
Based on the 4.5L volume of water in step 2), the weight of raw jujube in step 2) is 800g to 900g,
The weight ratio of the raw jujube mixed drink and the juice of the plant in step 4) is 20:2
Raw jujube mixed drink manufacturing method.
The method according to claim 1, wherein the raw jujube juice ground in step 1) is frozen at -18°C to -20°C and then pulverized.
A raw jujube mixed drink prepared by the method of claim 1.
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