KR102354728B1 - Production method of calcium acetate and calcium acetate water using ultrasonic waves and cosmetic composition including the calcium acetate water - Google Patents

Abstract

The present invention relates to a method for producing water-soluble calcium acetate. The method for preparing water-soluble calcium acetate according to the present invention includes a preparation step of adding 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate to a reaction vessel; a reaction step of preparing a suspension by ultrasonication in the reaction vessel; and a separation and purification step of obtaining a white powder by centrifuging the suspension prepared in the reaction step and drying it.

Description

Method for producing water-soluble calcium acetate and calcium acetate water using ultrasonic waves, and a cosmetic composition containing the same

The present invention relates to a method for producing water-soluble calcium acetate and calcium acetate water using ultrasonic waves, and a cosmetic composition containing the same. More specifically, the present invention can produce calcium acetate water having a cluster size of 40 to 60 Hz, which can not only produce high-purity water-soluble calcium acetate in a short time within 1 hour, but also increase intracellular absorption by using ultrasound. It relates to a method for producing water-soluble calcium acetate and acetate water using ultrasonic waves, which can be usefully used as a cosmetic composition, and a cosmetic composition containing the same.

In general, minerals such as calcium and magnesium are referred to as trace elements constituting the human body, and among them, calcium plays a role as a secondary messenger inducing water retention in skin cells. It plays a major role in increasing the synthesis of ceramide, one of the substances constituting the lipid layer of cells, by increasing the expression of glucosylceramide synthetase in the calcium-induced differentiation. Calcium is one of the minerals essential for the composition and activity of various living things, and in the case of animals, it is not only necessary for the composition and creation of bones and blood, but also plays a role in transmitting information to the heart and brain, and calcium in plants. is an important factor for meristem growth and root development.

Calcium goes through various metabolic processes, about 20% to 30% is absorbed in the body, and the rest is excreted in feces. In order for calcium ingested from food or preparations to be absorbed into the body, it is first dissolved in the stomach and ionized into ^{calcium ions (ca 2+ ).} That is, the faster the dissolution rate of calcium and the higher its ionization degree, the higher the absorption and utilization rate of calcium. Technology that can increase absorption and utilization in the body is considered a key technology.

Calcium in the skin plays a very important role in the upper layer of the skin, the epidermis, and calcium in the epidermis controls enzymes and lipids to prevent drying of the skin, and the lipid barrier protects the skin by producing lipids according to the amount of calcium. Prevents evaporation of moisture. In addition, the lack of calcium interferes with the exfoliation of dead skin cells in the process of skin generation, thereby significantly affecting the regeneration activity of the skin. Calcium regulates the rate of regeneration in the epidermis and accelerates the rate of replacement with new cells. If calcium is insufficient, new cells cannot be formed quickly, making the skin weak and dry. Calcium not only activates melanocytes, but also activates melanocytes It also acts as an antioxidant for cells, so if the skin is too oily, it dries easily and becomes sensitive to external stimuli, which accelerates skin aging and damage.

In The pathophysiology of vulnerable skin, "calcium ion concentration in the skin is that the concentration is dependent on the location, the concentration of calcium ions toward the upper layer is gradually increased, the misorientation screen of the skin which is located choesangcheungbu skin is Ca ²⁺ Signal transduction and differentiation of keratinocytes occur simultaneously by ions” (Source: The pathophysiology of vulnerable skin _ World wide wounds). As such, the role of calcium ions in the body or in the skin is studied through various academic data to show the effects of strengthening the skin barrier, regenerating the skin, and moisturizing the skin by participating in the biochemical processes in the body according to the calcium ion gradient. Furthermore, it exhibits a moisturizing effect by maintaining the moisture of the skin by the inflow of glycerin and H ₂ O into the cells, and the transepidermal water loss due to skin damage, that is, lipids are synthesized by the increase of TEWL (Transepidermal water loss). Many studies have been conducted on the effect of strengthening the skin barrier and promoting skin regeneration by promoting the differentiation of keratinocytes according to the change in the calcium ion gradient (Source: The pathophysiology of vulnerable skin_world wide wounds, J. Soc Cosmet Sci. Korea eISSN 2288-9507 Vol. 42, No. 4, December 2016, 343-349, J. Soc. Cosmet. Scientists Korea, Vol. 39, No. 1, 2013 3. 39-46. ., J. Aesthet. Cosmetol., Vol. 13 No. 6, 713-720, December 2015).

When the skin barrier is damaged, the concentration of calcium ions in the epidermis changes, the lipid synthesis of the epidermis increases, the secretion of lamellar bodies increases, a lipid bilayer is formed between the keratinocytes, and the DNA synthesis of the basal layer increases, eventually resulting in the skin Barrier damage is restored. In addition, substances called cytokines are secreted, among which primary cytokines, tumor necrosis factor (TNF), interleukin (IL)-1, and IL-6, are important. However, if the barrier damage continues and these cytokines are chronically elevated, inflammation, epidermal proliferation, and grinding may also bring harmful results (Source: Kor., J. Aesthet. Cosmetol., Vol. 13 No. 6, 713). -720, December 2015).

Calcium, vitamin D, and retinoic acid are known as factors affecting the differentiation of keratinocytes. Among them, calcium is best known as a bio factor that promotes the differentiation of keratinocytes, and the calcium concentration increases toward the top of the epidermis, with a low basal layer and a high granular layer. At a low concentration of calcium ion, keratinocytes proliferate, and when the concentration of calcium ions is high, cell proliferation stops and differentiation occurs by inducing the expression of differentiation marker genes (Hwang, 2000). As an essential element of skin barrier homeostasis regulation, it is important for maintaining moisture in the skin (Source: Kor., J. Aesthet. Cosmetol., Vol. 13 No. 6, 713-720, December 2015).

Ion calcium has been recognized for its superiority and is being developed as a food additive or health functional food in various forms. For food additives, various chemical preservatives or some natural products are used to prevent bacterial growth and suppress gas generation for efficient preservation of food. As the research result was announced that good skin with a lot of moisture contains a lot of calcium, research on the effect of calcium on the skin has been steadily conducted. Calcium, which is said to be as good for skin as eating, is expanding beyond health supplements to cosmetics (source: Euromonitor, International Trade Association (MTI 2273)).

In the cosmetic industry, as a cosmetic, it is regarded as a functional cosmetic when it has excellent skin protection and skin function, as well as the ability to correct cosmetic defects (wrinkles, wounds, spots, pigmentation, etc.). The cosmetics market is growing rapidly around the world, and recently, skin moisturizing, skin barrier strengthening, skin regeneration and functional cosmetic effects (whitening, wrinkle, UV protection) are emphasized using natural cosmetic raw materials. , the chemical component gradually disappears, and the natural raw material component is increasing.

Recently, in the domestic cosmetic market, various cosmetics using mineral water, which have the effect of increasing absorption into the skin, moisturizing the skin, and strengthening the skin barrier, are being launched. Among them, “Ion Calcium Ultra Rich Cream” released by Mineral Bio is the most representative. Manyo Factory's "Hot Spring Water Mineral Cream" and Innisfree's "Jeju Carbonated Mineral Mist" have been released, and a variety of product lines are still being developed.

Although the cosmetic market using calcium ions is gradually increasing, most of the calcium powder used as a means to supply calcium ions is insoluble, and although some are water-soluble and used, the solubility is also significantly lowered. is showing Even now, studies on the solubilization of insoluble calcium powder are being conducted in many places, and if mass-production of water-soluble calcium powder with high solubility becomes a general industry, it can be expanded not only in cosmetics but also in the food market, so the market using water-soluble calcium powder will gradually increase in the future is expected to grow to

As a substance that maintains homeostasis in the body, solubilization is absolutely necessary to absorb calcium ions, which are important factors. General calcium powder is insoluble, so the water solubility for cosmetic raw material needs to be increased by using acid to increase the hydrophilicity, and the effect of calcium is maximized when supplied in the form of ions, so the ionization rate of the powder is 90 % should be maintained.

In order to increase the absorption of calcium ions, the smaller the water molecule cluster size of the ionized calcium mineral water, the greater the absorption into the skin. A water molecule does not consist of a single molecule alone, and at most 1,000 or more molecules are gathered to form a cluster. The size of various water molecules is about 120 Hz for tap water, 110 Hz for distilled water, 100 Hz for groundwater, and 80 Hz for longevity villages. The size of human cells is about 60Hz, and in the case of mineral water containing calcium ions, the smaller the size of the cells, the higher the absorption rate. In addition, it is very important to maintain the size of the water molecule cluster for a long time because the size of the small water molecule cluster also increases over time.

Domestic calcium applications are mostly used in health functional foods and calcium-fortified foods. Representative companies include KMF Co., Ltd. and Eco Biotech. It has the technology to manufacture and the ionization rate is 96%. However, in the case of a technology using fermentation, it takes a lot of time because it ferments at least 72 hours.

Ecobiotech Co., Ltd. has the technology to produce calcium oxide by calcining oyster shell-derived calcium carbonate and electrolyzing it to produce water-soluble calcium, boasting a purity of 95%. However, the above technique has a problem in that price competitiveness is lowered because a high-temperature calcination process of 1400° C. or higher is required to obtain calcium oxide and expensive equipment for electrolysis is required.

Accordingly, the present inventors have conducted research for many years to develop water-soluble calcium, and as a result, calcium acetate water having a cluster size of 40 to 60 Hz that can be produced in a short time within 1 hour and can increase the intracellular absorption rate can be prepared. In addition, the present invention has been completed by completing the development of technology that can be usefully used in cosmetic compositions and the like due to excellent preservation of the prepared calcium acetate water.

KR registered patent 10-1093007 KR registered patent 10-1356745 KR Patent Publication : 10-2007-00008297 KR Patent Publication: 10-2013-0106802 KR Patent Publication : 10-2016-0026549

Accordingly, an object of the present invention is to provide a method for preparing water-soluble calcium acetate that can be prepared in a short time and has an excellent ionization rate.

Another object of the present invention is to provide a method for preparing calcium acetate water having a cluster size of 40 to 60 Hz, which can be prepared in a short time and can increase the intracellular absorption, as well as excellent preservation.

Another object of the present invention is to provide calcium acetate water prepared by the above method and a cosmetic composition containing the same.

In order to solve the above problems, the present invention provides a preparation step of adding 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate to a reaction vessel; a reaction step of preparing a suspension by ultrasonication in the reaction vessel; and a separation and purification step of obtaining a white powder by centrifuging the suspension prepared in the reaction step and drying it.

The present invention also includes a preparation step of adding 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate to the reaction vessel; a reaction step of preparing a suspension by ultrasonication in the reaction vessel; a separation and purification step of centrifuging the suspension prepared in the reaction step and drying it to obtain a white powder, water-soluble calcium acetate; It provides a method for producing calcium acetate water, comprising: adding and dissolving the water-soluble calcium acetate obtained in the separation and purification step in distilled water, and circulating the solution obtained by dissolving it to a magnetization machine to make it magnetized.

In the separation and purification step, centrifugation may be repeated several times using ethanol.

The ultrasonic treatment in the reaction step may be carried out for 20 to 50 minutes.

In the magnetization step, the water-soluble calcium acetate may be dissolved by adding it to distilled water so as to be 0.05 to 0.1% by weight.

The magnetization may be circulating in a magnetizer such that the produced calcium acetate magnetized water has a cluster size of ^{40 to 60 Hz based on 17 O-NMR.}

The present invention also provides calcium acetate water, characterized in that produced by the above production method.

The present invention also provides a cosmetic composition containing the calcium acetate water.

In the present invention, not only water-soluble calcium acetate can be produced in a short time within 1 hour using ultrasound, but also calcium acetate water having a high ionization rate and a cluster size of 40 to 60 Hz that can increase the intracellular absorption rate can be prepared. , In addition, it has excellent preservation of the prepared calcium acetate water, and has effects such as skin moisture retention and keratin improvement, so it can be usefully used as a cosmetic composition.

1 is a graph showing the particle size of calcium carbonate and the prepared water-soluble calcium acetate measured using a particle size analyzer (PSA) before and after ultrasonic treatment.

The method for producing water-soluble calcium acetate according to the present invention includes a preparation step of injecting materials such as calcium carbonate and acetic acid into a reactor, a reaction step of preparing a suspension by irradiating ultrasonic waves to the reactor, and a separation and purification step of centrifuging and drying the suspension do.

The preparation step is to put 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate in the reaction vessel.

The solvent may be distilled water.

The acetic acid is added to prepare a water-soluble calcium acetate through the reaction with calcium carbonate, and 0.2 to 0.5 L per 100 g of calcium carbonate is preferably added. When the amount of acetic acid added is less than 0.2L, the reaction time may take a long time, and there is a problem that unreacted calcium carbonate remains. Therefore, it is preferable to add acetic acid within the above range.

The reaction step is to prepare a suspension by ultrasonication in the reaction vessel.

According to the present invention, ultrasonic waves are applied to the reaction between calcium carbonate and acetic acid, and when ultrasonic waves are applied, the calcium carbonate and acetic acid are rapidly reacted with particle grinding of calcium carbonate to prepare a suspension.

Substantially, looking at the particle distribution of the calcium carbonate particles and the prepared water-soluble calcium acetate before and after ultrasonication, it can be seen that the particle size is significantly reduced. 1 is a graph showing the particle size of calcium carbonate and the prepared water-soluble calcium acetate measured using a particle size analyzer (PSA) before and after ultrasonic treatment. As shown in FIG. 1 , it can be seen that the particle size is significantly reduced during ultrasonic treatment according to the present invention.

The ultrasonic treatment is preferably performed within 1 hour, more preferably from 20 minutes to 50 minutes. When sonicating for less than 20 minutes, a sufficient reaction is not achieved, and when sonicating for more than 50 minutes, an enhanced effect is no longer obtained.

In the separation and purification step, the suspension prepared in the reaction step is centrifuged and dried to obtain a white powder.

The centrifugation may be repeated several times using ethanol. If repeated several times using ethanol, unreacted acetic acid and impurities are dissolved in ethanol and separated.

When the separated precipitate is dried, a white powdery water-soluble calcium acetate powder can be finally obtained. Although drying is not limited, it is preferable to proceed sufficiently at 70-90°C.

As shown in Table 1, it can be seen that the water-soluble calcium acetate powder obtained in this way has a significantly shorter manufacturing time compared to the water-soluble calcium powder of other companies, thereby reducing the manufacturing cost.

division third party A third party B product of the present invention product Soluble Calcium Powder Soluble Calcium Powder Soluble Calcium Powder Technology Fermentation Firing, electrolysis ultrasound irradiation fair complication complication simple manufacturing time 6 days 21 days 1 hours

According to the present invention, there is provided a method for producing calcium acetate water using the water-soluble calcium acetate powder prepared according to the above production method.

The method for producing calcium acetate water according to the present invention includes a preparation step of adding 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate to a reaction vessel; a reaction step of preparing a suspension by ultrasonication in the reaction vessel; a separation and purification step of centrifuging the suspension prepared in the reaction step and drying it to obtain a white powder, water-soluble calcium acetate; and a magnetization step of adding and dissolving the water-soluble calcium acetate obtained in the separation and purification step to distilled water, and circulating the solution obtained by dissolving it in a magnetizing machine to make it magnetized.

In the above, the preparation step, the reaction step, and the separation and purification step are the same as the above-described method for preparing a water-soluble calcium acetate, so a detailed description thereof will be omitted. That is, since the method for producing calcium acetate water according to the present invention further includes a magnetization step of magnetizing using the water-soluble calcium acetate obtained according to the above-described method for producing water-soluble calcium acetate, it will be described in detail.

In the magnetization step, the water-soluble calcium acetate obtained in the separation and purification step prepared in the separation and purification step described above is added and dissolved in distilled water, and the solution obtained by dissolving is circulated to a magnetization machine to magnetize it.

The magnetizer can be used without any restrictions on what is generally sold. Magnetized water is in the _{state of very small H 2} O clusters, and penetration, dissolving power, and purification power are very activated. And is easy to penetrate into the microfine part of such capillary membranes, a low reduction potential, converting the harmful free radicals into harmless oxygen, but due to the strong hydrogen bonding strength of the H ₂ O increases by the amount (110 ~ 120Hz) in H ₂ O resilience Also, the ability to maintain the size of the magnetized water cluster is not guaranteed. If you check the representative liquid cluster size, milk is 210Hz, distilled water is 118Hz, rain water is 119Hz, mineral water is 94Hz, and hot spring water is 79Hz. When normal distilled water is magnetized, it maintains around 60Hz immediately after manufacturing, but after several months, the cluster size gradually returns to its original state as much as distilled water. However, the cluster size of the magnetized water manufactured according to the present invention maintains the cluster size immediately after manufacturing even after several months.

In the magnetization step, it is not necessary to limit the concentration of the water-soluble calcium acetate, but it is preferable to dissolve it by adding it to distilled water so as to be 0.01 to 1.0% by weight.

The magnetization may be circulating in a magnetizer such that the produced calcium acetate magnetized water has a cluster size of ^{40 to 60 Hz based on 17 O-NMR.}

Although there are some differences depending on the performance of the magnetizer, if the magnetization is circulated for 3 to 5 hours per 50 kg of the total manufactured magnetization number, calcium acetate number having a cluster size of 40 to 60 Hz based on ^{17 O-NMR can be obtained.}

The present invention provides calcium acetate water, characterized in that it is prepared by the above production method.

The calcium acetate water prepared according to the present invention maintains the cluster size immediately after production even after several months, and has excellent preservation properties.

The cosmetic composition prepared using calcium acetate water according to the present invention has effects such as skin moisture retention and keratin improvement. Accordingly, the calcium acetate water prepared according to the present invention can be usefully used in a cosmetic composition.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but these are only presented to help the understanding of the present invention, and the present invention is not limited thereto.

<Example 1> Preparation of water-soluble calcium acetate

To a glass vial containing 5 mL of distilled water, 0.1 g CaCO3 and 0.3 mL acetic acid were added and then sonicated for 30 min to obtain a suspension. For sonication, a Digital Sonifier 450 sonicator from Branson was used. The resulting suspension was repeatedly centrifuged with ethanol 5 times (3600 rpm per time, 1 min), and then dried in an electric oven at 80° C. to prepare a white powder, water-soluble calcium acetate.

<Experimental Example 1>

0.1 g of water-soluble calcium acetate prepared according to the method of Example 1 was dissolved in 10 mL of distilled water. For the comparative experiment, 0.1 g of commercially available water-soluble calcium powder (company C) was dissolved in 10 mL of distilled water.

After dissolution, calcium ion concentration, particle size and solubility (after 1 hour) were measured, and the results are shown in Table 2 below.

division Example 1 third party C product Soluble Calcium Powder Soluble Calcium Powder Calcium ion concentration (mL/L) 111.3 11.4 Particle size (um) 0.1~0.2 1-2 Solubility 100% 12%

As shown in Table 2, in the case of the water-soluble calcium acetate powder prepared according to the present invention, it can be seen that the calcium ion concentration is very high as well as the solubility is very excellent.

<Example 2> Preparation of calcium acetate water

0.1Kg of water-soluble calcium acetate prepared according to the method of Example 1 was put into 19.9Kg of distilled water to make a total of 20Kg, and then circulated while passing through a magnetizer (Ecomine). Circulated for 2 hours to prepare calcium acetate water according to the present invention.

<Experimental Example 2>

In order to measure the cluster size of the magnetized water prepared in Example 2, ^{analysis was carried out by 17} O-NMR analysis, a nuclear magnetic resonance analysis method (analysis equipment (JEOL, JeolJNM-LA400 with LFG), solvent (Deuterium Oxide) )). The analysis results for each period of magnetized water are shown in Table 3 below.

Sample name Analysis items unit Analysis method Analysis immediately after manufacturing half width Hz ¹⁷ O-NMR 56.5 1 month after manufacture half width Hz ¹⁷ O-NMR 55.3 2 months after manufacture half width Hz ¹⁷ O-NMR 57.1 4 months after manufacture half width Hz ¹⁷ O-NMR 55.2

As shown in Table 3, it can be seen that the calcium acetate water prepared according to the present invention has a cluster size of 40 to 60 Hz that can increase the intracellular absorption, as well as excellent preservation.

<Experimental Example 3> Skin efficacy test evaluation of calcium acetate water (Korea Institute of Dermatology)

For 21 adult women over 20 years of age, after washing the face in the morning and evening for 2 weeks, the magnetized water prepared in Example 2 was sprayed evenly on the face and face, and then the skin moisture retention, skin keratin level improvement and skin moisture loss amount were measured as follows. They were evaluated together, and the average value of the results is shown in Table 4 below.

- Maintain skin moisture (moisture measurement test)

Using Epsilon E100, skin moisturizing improvement was evaluated before use, immediately after one use, and after two weeks of use.

- Evaluation of keratin improvement

Using a video microscope and an image analysis program, the degree of skin keratin improvement in the left lip area was analyzed before use, immediately after one use, and after two weeks of use, and the results are shown in Table 4 below.

- Evaluation of skin moisture loss improvement

Using a vapometer, the degree of improvement in skin moisture loss before, immediately after, and after 2 weeks of use was analyzed, and the results are shown in Table 4 below.

Evaluation items before use Immediately after 1 use after 2 weeks of use change in skin moisture 18.69 27.15 24.63 change in skin keratin 7.97 1.64 2.83 Changes in transdermal water loss 11.78 10.31 10.66

As shown in Table 4, it can be seen that the calcium acetate water prepared according to the present invention helps to improve skin moisture compared to before use, and reduces the amount of dead skin cells and moisture loss in the skin. Therefore, it can be seen that the calcium acetate water prepared according to the present invention can be usefully used in a cosmetic composition.

<Example 3>

Using the calcium acetate water prepared in Example 2, toner, ampoule, and cream cosmetics were prepared with the compositions shown in Tables 5, 6 and 7 below.

toner ingredient content
(w/w%) ingredient content
(w/w%) Calcium acetate water (Example 2) 69.939 Hydrogenated Phosphatidylcholine 0.05 glycerin 12.0 Evening Primrose Oil 0.05 Butylene Glycol 6.0 cetearyl alcohol 0.05 pentylene glycol 3.0 Safflower Seed Oil 0.05 niacinamide 2.0 Dipotassium Glycyrinate 0.05 Bis-PEG-18 methyletherdimethylsilane 1.0 Ethylhexylglycerin 0.05 squalane 1.0 adenosine 0.04 diethoxyethyl succinate 1.0 Disodium EDIT 0.03 PEG/PPG-17/6 copolymer 1.0 Polyglyceryl-2 Stearate 0.03 PEG-60 Hydrogenated Castor Oil 0.5 Stearic Acid 0.02 Cetylethylhexanoate 0.5 tocopheryl acetate 0.01 Neopentyl glycol diheptanoate 0.3 Ceramide NP 0.009 Glyceryl Caprylate 0.3 Polyquaternium-51 0.005 allantoin 0.2 Hydroxypropyltriammonium hyaluronate 0.001 Calcium acetate (Example 1) 0.1 Hydrolyzed Hyaluronic Acid 0.001 Sodium Hyaluronate 0.1 Palmitoyl tetrapeptide-7 0.001 Purslane Extract 0.1 Palmitoyltetrapeptide-1 0.001 Arginine 0.1 Palmitoyl tetrapeptide-12 0.001 1,2-Hexanediol 0.1 Sodium hyaluronate crosspolymer 0.001 Carbomer 0.1 Sodium Acetylated Hyaluronate 0.001 Polyglyceryl 10 Myristate 0.1 Spices 0.05 ethanol 0.06

ample ingredient content
(w/w%) ingredient content
(w/w%) Calcium acetate water (Example 2) 71.381 toromethamine 0.05 Butylene Glycol 10.0 Hydroxyethyl Cellulose 0.05 glycerin 6.0 Ethylhexylglycerin 0.05 pentylene glycol 3.0 adenosine 0.04 Glyceres-26 3.0 Disodium EDIT 0.03 niacinamide 2.0 Hydrogenated polyisobutene 0.01 1,2-Hexanediol 1.0 Hydroxypropyltriammonium hyaluronate 0.001 betaine 1.0 Hydrolyzed Hyaluronic Acid 0.001 Glycosyl Trehalose 0.6 Palmitoyl tetrapeptide-7 0.001 Sodium Hyaluronate 0.5 Palmitoyltetrapeptide-1 0.001 panthenol 0.5 Palmitoyl tetrapeptide-12 0.001 allantoin 0.2 zymo root extract 0.001 PEG-60 Hydrogenated Castor Oil 0.2 Sodium hyaluronate crosspolymer 0.001 Calcium acetate (Example 1) 0.1 Sodium Acetylated Hyaluronate 0.001 Hydrogenated Starch Hydrolinate 0.1 tocopherol 0.001 Purslane Extract 0.1 Spices 0.03 Carbomer 0.05

cream ingredient content
(w/w%) ingredient content
(w/w%) Calcium acetate water (Example 2) 57.625 Purslane Extract 0.1 glycerin 12.0 Butylene Glycol 0.1 Pentaerythritol tetraisostearate 8.0 tromethamine 0.1 cyclopentasiloxane 4.0 tocopheryl acetate 0.1 squalane 4.0 Dipotassium Glycyrinate 0.1 niacinamide 2.0 cholesterol 0.1 Caprylic/Capric Triglycerides 1.0 lecithin 0.1 cetearyl alcohol 1.0 glucose 0.1 Cyclohexasiloxane 1.0 Acrylate/C10-C30 acrylate crosspolymer 0.05 1,2-Hexanediol 1.0 adenosine 0.04 Macadamia Seed Oil 1.0 Disodium EDIT 0.03 Bis-Stearyldimethicone 1.0 Dolconsterol 0.025 Glyceryl Stearate 1.0 Phytosphingosine 0.01 C14-C22 alcohol 0.5 Ceramide NP 0.01 dimethicone 0.5 tocopherol 0.001 panthenol 0.5 Sodium Hyaluronate 0.001 jojoba ester 0.5 Hydroxypropyltriammonium hyaluronate 0.001 PEG-100 Stearate 0.5 Hydrolyzed Hyaluronic Acid 0.001 Hydrogenated Lecithin 0.5 pentylene glycol 0.001 behenyl alcohol 0.3 Palmitoyl tetrapeptide-7 0.001 caprylic glycol 0.3 Palmitoyltetrapeptide-1 0.001 C12-C20 Alkylglucoside 0.2 Palmitoyl tetrapeptide-12 0.001 Carbomer 0.2 Sodium hyaluronate crosspolymer 0.001 Dimethicone/Vinyl Dimethicone Cross Polymer 0.15 Sodium Acetylated Hyaluronate 0.001 Calcium acetate (Example 1) 0.1 Spices 0.15

<Experimental Example 4>

In Example 3, skin efficacy and safety were evaluated for cosmetics in toner, ampoule, and cream formulations.

(1) Evaluation of skin efficacy of 3 formulations (Korea Institute of Dermatology)

For 21 voluntarily recruited adult women aged 20 to 60, after cleansing in the morning and evening for 2 weeks during the test period, apply the toner, ampoule, and cream prepared in Example 3 evenly over the face and both forearms of the test subject After application and absorption, skin moisture retention, skin keratin improvement, and skin moisture loss were evaluated in the same manner as in Experiment 3, and the average values of the results are shown in Table 8 below.

Evaluation items before use Immediately after 1 use after 2 weeks of use change in skin moisture 14.10 43.46 23.26 change in skin keratin 5.79 0.78 1.16 Changes in transdermal water loss 27.41 18.09 7.38

As shown in Table 8, in the case of toners, ampoules, and creams prepared using calcium acetate water according to the present invention, compared to before use, it helps to improve skin moisture and reduces the amount of dead skin cells and moisture loss in the skin. .

(2) Safety test evaluation of 3 formulations_Korea Institute of Dermatological Sciences

A skin patch test was conducted using Finn Chamber on 34 voluntarily recruited adult women over 20 years old. After wiping the subject's back with 70% ethanol and drying it, 20 μL of each of the toner, ampoule, and cream prepared in Example was dropped into a Finn Chamber with a diameter of 8 mm and attached to the test site to fix it. The patch was attached for 24 hours, and after 30 minutes, 24 hours, and 48 hours after removal of the patch, the degree of irritation was observed by a dermatologist according to the criteria of the International Contact Dermatitis Research Group (ICDRG), and the The results are shown in Table 9 below.

division number of responders Elapsed time after patch removal Average skin reaction 30 minutes 24 hours 48 hours toner One 0.0 0.0 0.5 0.16 ample One 0.0 0.0 1.0 0.33 cream 0 0.0 0.0 0.0 0.00

As shown in Table 9, it can be seen that the toners, ampoules and creams prepared using the calcium acetate water according to the present invention have almost no skin irritation and thus ensure safety.

In the above, the present invention has been focused on preferred embodiments, and those of ordinary skill in the art to which the present invention pertains can implement embodiments other than the detailed description of the present invention within the essential technical scope of the present invention. will be able Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

Claims (9)

delete delete delete A preparation step of adding 10 to 20 L of solvent and 0.2 to 0.5 L of acetic acid per 100 g of calcium carbonate to the reaction vessel;
A reaction step of preparing a suspension by ultrasonication in the reaction vessel;
a separation and purification step of centrifuging the suspension prepared in the reaction step and drying it to obtain a white powder, water-soluble calcium acetate;
A magnetization step of adding and dissolving the water-soluble calcium acetate obtained in the separation and purification step to distilled water, and circulating the solution obtained by dissolving it in a magnetizing machine to make it magnetized;
In the magnetization step, the water-soluble calcium acetate is dissolved by adding it to distilled water so as to be 0.05 to 0.1% by weight,
The method for producing calcium acetate water, characterized in that the magnetization is circulated through a magnetization machine so that the produced calcium acetate magnetized water has a cluster size of ^{40 to 60 Hz based on 17 O-NMR.}
delete delete [Claim 5] The method according to claim 4, wherein the ultrasonic treatment in the reaction step is performed for 20 to 50 minutes.
Calcium acetate water, characterized in that produced by the manufacturing method of claim 4 or 7.
A cosmetic composition containing the magnetized calcium acetate water of claim 8.

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