KR102306648B1 - 디지털 계수 방법의 개선 - Google Patents

디지털 계수 방법의 개선 Download PDF

Info

Publication number
KR102306648B1
KR102306648B1 KR1020197003323A KR20197003323A KR102306648B1 KR 102306648 B1 KR102306648 B1 KR 102306648B1 KR 1020197003323 A KR1020197003323 A KR 1020197003323A KR 20197003323 A KR20197003323 A KR 20197003323A KR 102306648 B1 KR102306648 B1 KR 102306648B1
Authority
KR
South Korea
Prior art keywords
analyte
capture
liquid
labeling
captured
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020197003323A
Other languages
English (en)
Korean (ko)
Other versions
KR20190035729A (ko
Inventor
안드레아스 야안 쿤딩
Original Assignee
셀마 디아그노스틱스 에이피에스
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2016/074045 external-priority patent/WO2017060457A1/en
Application filed by 셀마 디아그노스틱스 에이피에스 filed Critical 셀마 디아그노스틱스 에이피에스
Publication of KR20190035729A publication Critical patent/KR20190035729A/ko
Application granted granted Critical
Publication of KR102306648B1 publication Critical patent/KR102306648B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0042—Investigating dispersion of solids
    • G01N2015/0053—Investigating dispersion of solids in liquids, e.g. trouble
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0096—Investigating consistence of powders, dustability, dustiness
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/08—Investigating permeability, pore-volume, or surface area of porous materials
    • G01N2015/0866—Sorption
    • G01N2015/0873—Dynamic sorption, e.g. with flow control means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020197003323A 2016-07-29 2017-07-28 디지털 계수 방법의 개선 Active KR102306648B1 (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662368564P 2016-07-29 2016-07-29
US62/368,564 2016-07-29
PCT/EP2016/074045 WO2017060457A1 (en) 2015-10-07 2016-10-07 Flow system and methods for digital counting
EPPCT/EP2016/074045 2016-10-07
EP17165915 2017-04-11
EP17165915.4 2017-04-11
PCT/EP2017/069212 WO2018020024A1 (en) 2016-07-29 2017-07-28 Improvements in methods for digital counting

Publications (2)

Publication Number Publication Date
KR20190035729A KR20190035729A (ko) 2019-04-03
KR102306648B1 true KR102306648B1 (ko) 2021-09-30

Family

ID=61016440

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020197003323A Active KR102306648B1 (ko) 2016-07-29 2017-07-28 디지털 계수 방법의 개선

Country Status (8)

Country Link
US (1) US11035854B2 (enExample)
EP (1) EP3491385B1 (enExample)
JP (1) JP6925051B2 (enExample)
KR (1) KR102306648B1 (enExample)
CN (1) CN109661580B (enExample)
CA (1) CA3031303C (enExample)
DK (1) DK3491385T3 (enExample)
WO (1) WO2018020024A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3491385T3 (en) * 2016-07-29 2021-07-12 Selma Diagnostics Aps Improvements in methods for digital counting
US12085499B2 (en) * 2019-12-20 2024-09-10 Illumina, Inc. Flow cells
CN111273000A (zh) * 2020-02-21 2020-06-12 东南大学 一种数字elisa检测装置与检测方法
CN120771941B (zh) * 2025-09-12 2025-12-16 大连华微生命科技有限公司 一种n进制皮升或飞升级流体精准量取系统或反应体系

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080026379A1 (en) 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
WO2009029073A1 (en) 2007-08-30 2009-03-05 The Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.

Family Cites Families (52)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6849462B1 (en) 1991-11-22 2005-02-01 Affymetrix, Inc. Combinatorial strategies for polymer synthesis
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
AU2001238606A1 (en) 2000-02-18 2001-08-27 Board Of Trustees Of The Leland Stanford Junior University Apparatus and methods for parallel processing of micro-volume liquid reactions
US7153679B2 (en) 2000-07-26 2006-12-26 Aventis Behring Gmbh Marburg I mutant of factor VII activating protease (FSAP) as risk factor for arterial thrombosis
US20040018491A1 (en) 2000-10-26 2004-01-29 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
EP1556506A1 (en) 2002-09-19 2005-07-27 The Chancellor, Masters And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US7329545B2 (en) 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
FR2861609B1 (fr) 2003-10-31 2006-02-03 Commissariat Energie Atomique Procede de repartition de gouttes d'un liquide d'interet sur une surface
JP4423150B2 (ja) 2004-09-24 2010-03-03 有限責任中間法人 オンチップ・セロミクス・コンソーシアム 液滴サイズの制御装置ならびに制御方法
EP1901067A3 (en) 2004-08-03 2009-05-13 On-Chip Cellomics Consortium Cellomics system
EP2272983A1 (en) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
WO2007103558A2 (en) 2006-03-09 2007-09-13 The Regents Of The University Of California Hybrid energy transfer for nucleic acid detection
EP2084532A1 (en) 2006-10-30 2009-08-05 Koninklijke Philips Electronics N.V. Porous biological assay substrate and method and device for producing such substrate
GB0700189D0 (en) * 2007-01-05 2007-02-14 Univ Leicester Fluorescence labelling
JP2009068995A (ja) * 2007-09-13 2009-04-02 Panasonic Corp マイクロアレイ装置
US8093063B2 (en) 2007-11-29 2012-01-10 Quest Diagnostics Investments Incorporated Assay for detecting genetic abnormalities in genomic nucleic acids
DK2565279T3 (en) 2007-12-05 2015-02-16 Complete Genomics Inc Efficient base determination in sequencing reactions
GB0804024D0 (en) 2008-03-04 2008-04-09 Mir Kalim U Multiplex selection
US9180453B2 (en) 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
US20100075862A1 (en) 2008-09-23 2010-03-25 Quanterix Corporation High sensitivity determination of the concentration of analyte molecules or particles in a fluid sample
US8222047B2 (en) * 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
KR101065078B1 (ko) 2008-11-05 2011-09-15 삼성전자주식회사 바이오칩용 기판 및 그 제조 방법
WO2010117470A2 (en) 2009-04-10 2010-10-14 Pacific Biosciences Of California, Inc. Nanopore sequencing devices and methods
US8926904B2 (en) 2009-05-12 2015-01-06 Daniel Wai-Cheong So Method and apparatus for the analysis and identification of molecules
EP2480689A4 (en) 2009-09-24 2013-05-15 Seegene Inc DETECTION OF TARGET NUCLEIC ACID SEQUENCES USING CYCLIC EXONUCLEOLYTIC REACTIONS
GB2490847B8 (en) 2010-02-08 2014-10-22 Gania Technologies Inc Systems and methods for manipulating a molecule ina nanopore
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
EP2606336A1 (en) 2010-08-20 2013-06-26 ETH Zurich Microwell arrays for direct quantification of analytes on a flat sample
CN101935703B (zh) * 2010-08-27 2012-10-10 中国人民解放军第三军医大学第一附属医院 肠出血性大肠杆菌o157:h7多色量子点快速检测试剂盒及其检测方法
WO2012072822A1 (en) 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Microanalysis of cellular function
CN107502660B (zh) 2011-01-20 2021-08-10 华盛顿大学商业中心 进行数字测量的方法和系统
US20150065358A1 (en) 2011-03-30 2015-03-05 Verinata Health, Inc. Method for verifying bioassay samples
CN102890474B (zh) 2011-07-21 2014-11-26 中国科学院物理研究所 一种用于控制单个生物分子在纳米孔中运动的系统及方法
US9095852B2 (en) 2011-08-22 2015-08-04 The Regents Of The University Of California Multilayer high density microwells
CN102296116A (zh) * 2011-09-02 2011-12-28 北京大学 对dna目标序列进行信号放大和检测的方法
US20140287423A1 (en) 2011-10-28 2014-09-25 Life Technologies Corporaton Device and method for apportionment and manipulation of sample volumes
WO2013110146A2 (en) 2012-01-24 2013-08-01 Katholieke Universiteit Leuven Patterning device
CN104508492B (zh) 2012-05-25 2018-07-27 北卡罗来纳-查佩尔山大学 微流体装置、用于试剂的固体支持体和相关方法
WO2014001459A1 (en) 2012-06-29 2014-01-03 Danmarks Tekniske Universitet A method of charging a test carrier and a test carrier
JP6124205B2 (ja) 2012-07-20 2017-05-10 公益財団法人神奈川科学技術アカデミー 人工脂質膜形成装置および人工脂質膜形成方法
EP2888571B1 (en) * 2012-08-23 2022-12-21 Stemcell Technologies Inc. Methods and kits for rapid and reversible biomolecular labeling
US20160258020A1 (en) 2013-10-21 2016-09-08 The Regents Of The University Of California Enrichment and detection of nucleic acids with ultra-high sensitivity
US20160312265A1 (en) 2014-01-14 2016-10-27 Aptitude Medical Systems, Inc. Use of nucleic acid agents for ultra-sensitive digital detection and quantification of target molecules
CN104531853B (zh) 2014-12-12 2017-08-01 上海交通大学 经高密度纳米点阵列制备生物大分子单分子芯片的方法
CN104535769B (zh) 2014-12-12 2016-07-06 上海交通大学 经高密度纳米坑制备生物大分子单分子芯片的方法
AU2016243036B2 (en) 2015-04-03 2022-02-17 Abbott Laboratories Devices and methods for sample analysis
WO2017004463A1 (en) 2015-07-01 2017-01-05 Abbott Laboratories Devices and methods for sample analysis
US11479805B2 (en) 2015-08-21 2022-10-25 The General Hospital Corporation Combinatorial single molecule analysis of chromatin
WO2017048815A1 (en) 2015-09-14 2017-03-23 Singulex, Inc. Single molecule detection on a chip
DK3491385T3 (en) * 2016-07-29 2021-07-12 Selma Diagnostics Aps Improvements in methods for digital counting
JP2020515074A (ja) 2017-03-24 2020-05-21 カードラブ・アンパルトセルスカブＣａｒｄＬａｂ ＡｐＳ キャリアのアセンブリとそれに固定された複数の電気回路、およびその製造方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080026379A1 (en) 2006-07-31 2008-01-31 Siddiqi Suhaib M Nucleotide analogs
WO2009029073A1 (en) 2007-08-30 2009-03-05 The Trustees Of Tufts College Methods for determining the concentration of an analyte in solution.

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Lab on a Chip, vol. 12, pp. 4986-4991 (2012) 1부.*

Also Published As

Publication number Publication date
EP3491385B1 (en) 2021-04-07
EP3491385A1 (en) 2019-06-05
CN109661580B (zh) 2023-01-24
CN109661580A (zh) 2019-04-19
US11035854B2 (en) 2021-06-15
JP6925051B2 (ja) 2021-08-25
JP2019523419A (ja) 2019-08-22
US20190154673A1 (en) 2019-05-23
KR20190035729A (ko) 2019-04-03
DK3491385T3 (en) 2021-07-12
WO2018020024A9 (en) 2018-03-22
WO2018020024A1 (en) 2018-02-01
CA3031303A1 (en) 2018-02-01
CA3031303C (en) 2023-05-02

Similar Documents

Publication Publication Date Title
US11693001B2 (en) Flow system and methods for digital counting
HK1247662A1 (en) Flow system and methods for digital counting
CN115506034B (zh) 寡核苷酸编码的化学文库
EP2286217B1 (en) Single molecule loading methods and compositions
US10585091B2 (en) Digital assay for quantifying and concentrating analytes
KR102306648B1 (ko) 디지털 계수 방법의 개선
KR20230011907A (ko) 생물학/유전학의 검출, 분석, 정량화 및 시험을 위한 올리고뉴클레오티드 코딩된 화학적 라이브러리, 관련 시스템, 장치 및 방법
KR102911933B1 (ko) 디지털 계수를 위한 흐름 시스템 및 방법
HK40009010B (en) Improvements in methods for digital counting
WO2025254035A1 (ja) 複数種類の標的分子を検出する方法

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20190131

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
A302 Request for accelerated examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20200727

Comment text: Request for Examination of Application

PA0302 Request for accelerated examination

Patent event date: 20200727

Patent event code: PA03022R01D

Comment text: Request for Accelerated Examination

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20201119

Patent event code: PE09021S01D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20210629

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20210923

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20210924

End annual number: 3

Start annual number: 1

PG1601 Publication of registration
PR1001 Payment of annual fee

Payment date: 20240826

Start annual number: 4

End annual number: 4