KR102287369B1 - Composition for preventing hair loss - Google Patents

Abstract

Disclosed is a composition for preventing hair loss that can have an effect not only on male hair loss but also on female hair loss by a Robinia pseudoacacia extract and a Houttuynia cordata extract. The present invention provides the composition for preventing hair loss, which comprises the Robinia pseudoacacia extract, the Houttuynia cordata extract and a Viola verecunda flower extract.

Description

Composition for preventing hair loss

The present invention relates to a composition for preventing hair loss, and more particularly, to a composition for preventing hair loss that can have an effect not only on male pattern hair loss but also on female pattern hair loss, including extracts of acacia tree and Echo extract.

It is known that testosterone is involved in development, spermatogenesis, sexual desire, and the like, and the negative aspects of acne, sebum increase, hair loss, and benign prostatic hyperplasia are known to be caused by the involvement of dihydrotestosterone in the tissue. Among them, research on hair loss, which occurs a lot in men, has been conducted for a long time, but the mechanism of hair loss and hair growth has not yet been accurately elucidated.

Hair is a living organism that regularly repeats the cyclical changes of growth, regression, and resting phases, and since each hair repeats independent creation and extinction, it always maintains a constant number of hairs. The number of hair is determined from birth, but after puberty, the process of hair growth due to the influence of sex hormones is often mistaken for an increase in the number. The growth phase is the period in which hair grows due to active cell division and activity of hair follicles, and it lasts for about 2-6 years, accounting for about 85% of the total hair. During the growth phase, hair follicle cells are surrounded by dermal papilla of stromal cells and have a circular shape, and cell division for the generation of new hair is active. The catagen stage is a period in which hair follicle growth activity stops and rapidly atrophys, and the shape of the hair at this time is similar to a club. It takes about 2-3 weeks and accounts for about 5% of the total hair. The resting phase is a period in which the cell activity of the hair follicles completely disappears. During the period of 2-3 months, it is pushed out by the growing hair that grows from the base of the resting hair, or naturally falls out by mechanical action such as combing or washing the hair. . In general, alopecia refers to an increase in the number of abnormal hair loss due to the shortening of the proportion of hair in the growth phase and more hair in the degenerative phase or telogen phase during this cycle.

The causes of alopecia, in which hair falls out from the scalp, are various, and are broadly divided into male hormone action, mental stress, lipid peroxide accumulation in the scalp, drug side effects, chronic diseases such as leukemia or tuberculosis, side effects caused by radiation therapy, nutrition deficiency, etc. In addition, in recent years, hair loss, which has been known to be a problem for men, is increasing in demand for prevention and treatment of female hair loss and young people's hair loss.

Drugs currently used as hair growth and hair growth agents include vasodilators to circulate sufficient blood to the scalp, female hormones to inhibit the action of male hormones, and 5α- to convert testosterone into 5-dihydrotesteone (5-DHT). There are testosterone activity inhibitors that inhibit reductase. The vasodilators include capronium chloride, minoxidil, and various plant extracts, the female hormone agents include estrogen, estradiol, and progesterone, and the male hormone activity inhibitors include finasteride and pentadecanoic acid.

However, due to insufficient effects or side effects of the treatment for hair loss, there is a need for a more effective and safe treatment for hair loss or development of a drug for hair growth. Minoxidil administered for topical or oral use causes irritation to the skin such as erythema, inflammation, infection, irritation, or pain of the scalp. There are problems such as In addition, finasteride has disadvantages such as erectile dysfunction and a decrease in sexual desire due to the effect of inhibiting male hormone activity.

(0001) Republic of Korea Patent No. 10-1039439 (0002) Republic of Korea Patent Registration No. 10-1797667

The present invention has been devised to solve the problems described above, and an object of the present invention is to provide an anti-hair loss composition comprising an acacia tree flower extract and an extract of the hornwort plant.

Another object of the present invention is to provide an anti-hair loss composition that can improve hair loss while minimizing skin irritation by including a natural extract.

The problems of the present invention are not limited to the problems mentioned above, and other problems not mentioned will be clearly understood by those skilled in the art from the following description.

In order to solve the above problems, the present invention provides an anti-hair loss composition comprising an acacia tree extract, an eoseongcho extract and a soybean violet flower extract.

In one embodiment, the anti-hair loss composition comprises 10 to 15 parts by weight of an acacia tree extract, 5 to 10 parts by weight of an eosinth extract, 5 to 10 parts by weight of a soybean flower extract, 5 to 10 parts by weight of a perilla leaf extract compared to 100 parts by weight of water. It contains 10-15 parts by weight and 10-15 parts by weight of the black bean extract, wherein the acacia tree extract comprises the steps of pulverizing an acacia tree branch to a size of 1-5 cm; swelling the pulverized acacia tree branches at a temperature of 100 to 300° C. and a pressure of 1 to 2 MPa; mixing 50 to 200 parts by weight of dry powder of acacia leaves based on 100 parts by weight of the swollen acacia tree branch; charging a mixture of the swollen acacia tree branch and dry powder of acacia tree leaves into an extraction container, and then supplying water vapor at 500 to 800° C. for 3 to 10 hours for high pressure extraction; And 30-50 parts by weight of the acacia flower extract is mixed with 100 parts by weight of the extract generated in the high-pressure extraction step to prepare an akashi tree extract, wherein the akashi flower extract is compared to 100 parts by weight of akashi flower mixing 50 to 150 parts by weight of sugar; charging the akashi flowers mixed with the sugar into a fermentation vessel; The step of sealing the fermentation vessel loaded with the akishi flower, and then extracting at a temperature of 10 to 20 ℃ for 30 to 50 days; And after the extraction is completed, it is prepared by a method comprising the step of preparing an acacia flower extract by removing the solids, the eoseongcho extract, removing the roots of the harvested eoseongcho, and then washing; Decomposing the cellulose of eoseongcho by injecting 100 to 300 parts by weight of water compared to 100 parts by weight of the washed eoseongcho and supplying ultrasonic waves having a frequency of 10 to 70kHz at an intensity of 500 to 1000W for 5 to 20 minutes; heating the eoseongcho in which the cellulose is decomposed at a temperature of 90 to 110° C. for 10 to 120 minutes; And after the heating is completed, it is prepared by a method comprising the step of separating the solid content and the eoseongcho extract, the soybean violet extract, the step of mixing 50 to 150 parts by weight of sugar compared to 100 parts by weight of the soybean violet; charging the soybean violet mixed with the sugar into a fermentation vessel; The step of sealing the fermentation vessel in which the soybean violet is charged, and then extracting at a temperature of 20 to 30° C. for 40 to 60 days; and after the extraction is completed, removing the solids to prepare a soybean violet flower extract, wherein the perilla leaf extract is washed with the collected perilla leaf and then dried to less than 5% moisture content ; pulverizing the dried perilla leaf to prepare perilla leaf powder; mixing 200 to 300 parts by weight of water to 100 parts by weight of the perilla leaf powder, then heating the mixture at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 30 to 80 minutes; and separating the perilla leaf extract and the solid content after the heating is completed; mixing with 80 to 150 parts by weight of water based on 100 parts by weight of the soaked black bean, then heating at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 10 to 30 minutes; After the heating is completed, pressing the black beans; And after the compression is completed, it is prepared by a method comprising the step of separating the black bean extract and the solid content, and the anti-hair loss composition is an acacia tree extract prepared by the above method in water, an eosincho extract, a soybean violet flower extract, a purple preparing an extract mixture by mixing the leaflet extract and the black bean extract; mixing 1 to 5 parts by weight of an oligosaccharide with respect to 100 parts by weight of the extract mixture; Inoculating the lactic acid bacteria in the extract mixture in which the oligosaccharide is mixed, and culturing for 1 to 10 days at a temperature of 20 ~ 30 ℃; mixing 1 to 5 parts by weight of ground hops in the extract mixture solution in which the lactic acid bacteria culture is completed; Inoculating brewer's yeast into the extract mixture in which the ground hops are mixed, opening the lid, and then culturing the primary culture for 5 to 10 days at a temperature of 5 to 30 ℃; After the primary culture is completed, sealing and secondary culturing for 3-5 days at a temperature of 5-30 °C; and sterilizing the extract mixture at a temperature of 50 to 70° C. for 10 to 30 minutes after the secondary culture is completed; It is prepared by a method comprising , concealer, BB cream, CC cream, shampoo, soap, hair dye, perm, wax or spray formulation.

According to an embodiment of the present invention, it is possible to provide an anti-hair loss composition capable of minimizing side effects while having a hair loss prevention effect similar to that of a conventional hair loss treatment agent by including the extract of acacia tree flower and eosinus plant.

In addition, since the present invention uses a natural herbal medicine as a main raw material, it is possible to provide a hair loss prevention composition that can minimize skin irritation.

In addition, the present invention can provide an anti-hair loss composition that maximizes the effect of preventing hair loss by applying and using an extraction method capable of maximizing the active ingredients of each herbal medicine.

The effect according to the present invention is not limited by the contents exemplified above, and more various effects are included in the present invention.

1 schematically shows a method for preparing a hair loss prevention composition according to an embodiment of the present invention.

Hereinafter, various embodiments will be described in more detail with reference to the accompanying drawings. The embodiments described herein may be variously modified. Certain embodiments may be depicted in the drawings and described in detail in the detailed description. However, the specific embodiments disclosed in the accompanying drawings are only for easy understanding of various embodiments. Therefore, the technical spirit is not limited by the specific embodiments disclosed in the accompanying drawings, and it should be understood to include all equivalents or substitutes included in the spirit and scope of the invention.

Terms including an ordinal number, such as first, second, etc., may be used to describe various elements, but these elements are not limited by the above-described terms. The above terminology is used only for the purpose of distinguishing one component from another component.

In this specification, terms such as "comprises" or "have" are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or more other features It should be understood that this does not preclude the existence or addition of numbers, steps, operations, components, parts, or combinations thereof. When a component is referred to as being “connected” or “connected” to another component, it is understood that the other component may be directly connected or connected to the other component, but other components may exist in between. it should be On the other hand, when it is said that a certain element is "directly connected" or "directly connected" to another element, it should be understood that no other element is present in the middle.

Meanwhile, as used herein, a “module” or “unit” for a component performs at least one function or operation. And “module” or “unit” may perform a function or operation by hardware, software, or a combination of hardware and software. In addition, a plurality of “modules” or a plurality of “units” other than a “module” or “unit” that must be performed in specific hardware or are executed in at least one processor may be integrated into at least one module. The singular expression includes the plural expression unless the context clearly dictates otherwise.

In addition, in describing the present invention, when it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description thereof will be abbreviated or omitted.

1 schematically shows a method for preparing an anti-hair loss composition of the present invention.

The present invention relates to an anti-hair loss composition comprising an acacia tree extract, an eosincho extract and a soybean violet extract.

The acacia tree is a deciduous tree belonging to the Rosaceae legumes, and is generally called an acacia tree, but refers to a species different from acacia of the mimosa family. This akashi tree is the origin of birch, and has been planted in large quantities for the purpose of using it for forest reforestation using strong fertility since the 1900s.

In the case of the present invention, it is possible to provide an anti-hair loss composition having an effect of improving hair loss by using the extract of the acacia tree, and adding a unique fragrance of the acacia tree. To this end, the acacia tree extract is pulverized to a size of 1 to 5 cm akashi tree branches; swelling the pulverized acacia tree branches at a temperature of 100 to 300° C. and a pressure of 1 to 2 MPa; mixing 50 to 200 parts by weight of dry powder of acacia leaves based on 100 parts by weight of the swollen acacia tree branch; charging a mixture of the swollen acacia tree branch and dry powder of acacia tree leaves into an extraction container, and then supplying water vapor at 500 to 800° C. for 3 to 10 hours for high pressure extraction; And it can be prepared by a method comprising the step of mixing 30-50 parts by weight of the acacia flower extract with 100 parts by weight of the extract generated in the high-pressure extraction step to prepare an acacia tree extract.

The acacia tree can be largely divided into branches, leaves, and flowers that can be used. However, since these three parts have different shapes, hardness, and texture, it is preferable to appropriately use an extraction method for each part.

First, the collected acacia tree branches can be crushed to a size of 1 to 5 cm, and then expanded at a temperature of 100 to 300 ° C and a pressure of 1 to 2 MPa. The acacia tree branch has a hard tissue strength, and it is not easy to extract the active ingredient by a general hot water extraction method. Therefore, it is preferable to use the branch of the acacia tree by pulverizing it to a small size and then expanding it using a expander. At this time, the expansion is preferably performed within the temperature and pressure range, and if the temperature and pressure range is less than the temperature and pressure range, the expansion is not smooth, so it may be difficult to extract the active ingredient. Equipment costs may be high due to carbonization or excessive pressure.

The acacia tree branches swollen as described above may be mixed with the acacia leaf dry powder. The dry powder of akashi leaves is prepared by drying and pulverizing the leaves of akashi, and in the case of the leaves of akashi, the tissue is softer than that of the branches of the acacia tree, so the dry powder can be used as described above.

The mixture of the above and the swollen acacia tree branch and dry acacia tree leaf powder can be extracted by high pressure by supplying water vapor at 500 to 800° C. for 3 to 10 hours after being put into an extraction container. At this time, the extraction vessel is provided with a multi-stage tray installed inside the main body of the extraction vessel, so that the mixture of the swollen acacia tree branch and dry acacia leaf dry powder can be loaded in multiple stages, and the multi-stage tray has a porous structure. It has a structure so that the water vapor that is formed can easily pass through the mixture of the swollen acacia tree branch and dry acacia leaf powder. Through this, the active ingredient of the mixture of the swollen acacia tree branch and the dry powder of the acacia tree leaf is eluted in the water vapor and can be recovered by falling to the lower part of the extraction container. At this time, when the temperature of the water phase is less than 500 ° C, it is not easy to extract the mixture of the swollen acacia tree branch and the dry acacia leaf powder, and when the water vapor exceeding 800 ° C is supplied, the swollen akashi tree branch and acacia It may be difficult to extract the active ingredient because the mixture of dry tree leaf powder is carbonized.

In addition, the extraction using the water vapor may be performed at high pressure in order to increase the extraction amount of the active ingredient contained in the mixture of the swollen acacia tree branch and dry acacia leaf powder. In this case, the extraction is preferably performed at 200 to 500 kPa. When the extraction is performed at less than 200 kPa, the extraction of the active ingredient is not smooth, and when it is performed at a pressure exceeding 500 kPa, the extraction device is manufactured to withstand high pressure, which requires a lot of manufacturing cost.

The extracts of the branches and leaves of the akashi tree prepared as described above may be used in combination with the extracts of the akashi flowers. At this time, it is preferable to use the liquid extraction method, not the steam extraction, as described above, because the salsi akashi flower has a softer tissue than the branches and leaves of the acacia tree.

To this end, the akashi flower extract is mixed with 50 to 150 parts by weight of sugar compared to 100 parts by weight of the akashi flower; charging the akashi flowers mixed with the sugar into a fermentation vessel; The step of sealing the fermentation vessel loaded with the akishi flower, and then extracting at a temperature of 10 to 20 ℃ for 30 to 50 days; And after the extraction is completed, removing the solids may be prepared by a method comprising the step of preparing an acacia flower extract.

The collected akashi flowers may be washed and then mixed with 50 to 150 parts by weight of sugar compared to 100 parts by weight of the akashi flowers. At this time, when the sugar is mixed in less than 50 parts by weight, it may be deteriorated or spoiled due to the low sugar content, and when it exceeds 150 parts by weight, the sugar component may be relatively increased and the content of the active ingredient may be decreased.

Akashi mixed with sugar as described above is charged into the fermentation vessel, then the fermentation vessel is sealed and can be extracted for 30 to 50 days at a temperature of 10 to 20 °C. At this time, since a high concentration of sugar component is located on the outside of the akishi flower, the active ingredient of the akishi flower can be eluted to the outside of the akashi flower by osmotic pressure, and also due to the action of the active ingredient and the eluted water, the The sugar melts and forms a liquid. At this time, when the extraction is less than the above temperature or less than 30 days, the active ingredient does not completely elute, so the content of the active ingredient may drop, and when it is eluted at a temperature exceeding 20 ° C or eluted for more than 50 days, an off-flavor may occur. .

After the extraction is completed, the solid content, that is, the debris of the acacia flower is removed, and then mixed with an extract of the swollen acacia tree branch and dried akashi tree leaf powder to prepare an acacia tree extract.

The acacia tree extract may be mixed with 10 to 15 parts by weight based on 100 parts by weight of the water. When the acacia tree extract is mixed in less than 10 parts by weight, the anti-hair loss effect may be reduced, and the akashi flavor of the anti-hair loss composition may fall, and when used in excess of 15 parts by weight, the overall preference decreases due to the excessive akashi flavor. can be

The Eoseongcho is a perennial grass belonging to the family Trifoliumaceae and its official name is Yakmomil. These fishworts are used as medicinal plants with fishy smell, and known ingredients include decanoyl actaldehyde, pinene, linalool, camphene, limonene, and cordarine ( cordarine), quercitrin, quercetin, isoquercitrin, and the like, are known to be effective in antibacterial action, antiviral action, antipyretic action and diuretic action. In addition, the eoseongcho of the present invention can have the effect of reducing the activity of a hair loss enzyme (Dihydrotestosterone, DHT) to prevent hair loss, and by performing a detoxification action through improvement of blood circulation to remove skin toxins and relieve inflammation.

However, in the case of eoseongcho, it is preferable to extract the active ingredient by using a method different from that of the akashi tree because it is an herbaceous plant unlike the akish name.

To this end, the eoseongcho extract removes the roots of the harvested eoseongcho, and then washing; Decomposing the cellulose of eoseongcho by injecting 100 to 300 parts by weight of water compared to 100 parts by weight of the washed eoseongcho and supplying ultrasonic waves having a frequency of 10 to 70kHz at an intensity of 500 to 1000W for 5 to 20 minutes; heating the eoseongcho in which the cellulose is decomposed at a temperature of 90 to 110° C. for 10 to 120 minutes; And after the heating is completed, it may be prepared by a method comprising the step of separating the solid content and the Eoseongcho extract.

The Eoseongcho may be collected and then washed by removing the roots. In this process, residues such as soil and insects present on the surface of the eoseongcho may be removed.

100 to 300 parts by weight of water may be injected into the washed eoseongcho compared to 100 parts by weight of eoseongcho. In this case, the water may be used not only as a solvent for extracting the active ingredient, but also as a medium for transmitting ultrasonic waves, which will be described later. When the water is used in less than 100 parts by weight, extraction of the active ingredient may be difficult, and when the water is used in excess of 300 parts by weight, the effective ingredient may be diluted and the effect may be reduced.

As described above, ultrasonic waves having a frequency of 10 to 70 kHz are supplied to the water-injected eoseongcho at an intensity of 500 to 1000W for 5 to 20 minutes to decompose the cellulose of the eoseongcho. Since the Eoseongcho is a kind of plant, a cell wall surrounds the cell circumference. In the case of such a cell wall, although it is a major organ forming the structure of the plant, it may interfere with extracting the active ingredient as in the present invention. Therefore, by decomposing the cellulose, which is the main component of the cell wall, using ultrasonic waves, the active ingredient of the eoseongcho can be extracted as much as possible.

At this time, since the ultrasonic wave decomposes the cellulose through a resonance phenomenon, it may have a frequency of 10 to 70 kHz. When the frequency of the ultrasonic wave is out of the above range, the decomposition of the cellulose cannot be performed. In addition, if the ultrasonic wave has an intensity of less than 500 W or is supplied for less than 5 minutes, the decomposition effect of the cellulose may be reduced. There is a possibility.

As described above, eoseongcho, in which cellulose is decomposed by supplying ultrasonic waves, can be heated at a temperature of 90 to 110° C. for 10 to 120 minutes to extract active ingredients. Through this process, it is possible to elute the active ingredient present in the cells of the Eosongcho, which can increase the content of the active ingredient compared to the existing elution method using hot water. At this time, when the temperature is less than 90 ℃ or heated for less than 10 minutes, the extraction amount of the active ingredient may be reduced, and when heated to a temperature exceeding 110 ℃ or heated for more than 120 minutes, there is no further extracting effect of the active ingredient The amount of energy required will increase.

After the extraction is completed as described above, the solid content may be separated to prepare an extract of Eoseongcho. The eosinth extract may contain 5 to 10 parts by weight based on 100 parts by weight of water in the anti-hair loss composition. When the eoseongcho extract is included in less than 5 parts by weight, it is difficult to expect the effect of the eoseongcho extract, and when it is mixed in excess of 10 parts by weight, it may be difficult to use the anti-hair loss composition due to the peculiar smell of eoseongcho.

The bean swallow flower is a flower of a perennial herb belonging to the violet family, the bean swallow flower, and means a white locust blooming in April to May. This soybean violet is widely distributed in Korea, China and Japan, and the flower has the effect of increasing the expression of cyclin D1 protein, which activates the proliferation cycle of dermal papilla cells, and decreasing the expression of p27 protein, which inhibits the proliferation cycle. It can be used as an anti-hair loss composition.

The bean violet may be extracted using a method similar to the akashi flower. However, some conditions may be different for the effective extraction of the bean violet active ingredient.

To this end, the bean swallow flower extract is mixed with 50 to 150 parts by weight of sugar compared to 100 parts by weight of the bean swallow flower; charging the soybean violet mixed with the sugar into a fermentation vessel; The step of sealing the fermentation vessel in which the soybean violet is charged, and then extracting at a temperature of 20 to 30° C. for 40 to 60 days; And after the extraction is completed, it may be prepared by a method comprising the step of removing the solids to prepare a soybean violet extract.

The perilla leaf is a leaf of a leaflet (Perilla frutescens BRITT. var acuta KUDO) or a leaflet wrinkled (Perilla frutescens BRITT. var crispa DECNE) belonging to the family Lamiaceae (labiatae), as known ingredients, perillaaldehyde, limonene ( limonene), arginine, cumic acid, isoegomaketone, and perilla alcohol are known and are known to be used to treat chills and chills. The perilla leaf helps to soothe the skin, and contains a lot of potassium that stimulates water metabolism, so it discharges toxins. In addition, it has the effect of inhibiting the hair loss enzyme, so it is possible to prevent hair loss.

Since the perilla leaf corresponds to a herbaceous plant like the eoseongcho, it is possible to extract it using an ultrasonic method as in the extraction method of eoseongcho. It is preferable to use

To this end, the perilla leaves are washed with the collected perilla leaves and then dried to a moisture content of less than 5%; pulverizing the dried perilla leaf to prepare perilla leaf powder; mixing 200 to 300 parts by weight of water to 100 parts by weight of the perilla leaf powder, then heating the mixture at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 30 to 80 minutes; and separating the perilla leaf extract and the solid content after the heating is completed.

The perilla leaves may be collected and then dried to a moisture content of less than 5%. At this time, if the moisture content of the perilla leaf is more than 5%, it may be difficult to grind the perilla leaf, so it is preferable that the perilla leaf is dried to have a moisture content of less than 5%. At this time, although hot air drying may be used for the drying, it is preferable to use low smoke drying or reduced pressure drying in order to prevent deterioration or thermal damage of the active ingredient.

The dried perilla leaves may be pulverized and prepared into powder. At this time, since the perilla leaf is in a dried state, it can be easily manufactured into a powder, and is preferably manufactured as a powder using a rotary grinder or a ball mill.

The perilla leaf powder prepared as described above may be extracted by mixing 200 to 300 parts by weight of water to 100 parts by weight of perilla leaf powder, and then heating it at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 30 to 80 minutes. At this time, the water is used as a solvent for extracting the active ingredient of the perilla leaf. When used in an amount of less than 200 parts by weight compared to 100 parts by weight of the perilla powder, it may be difficult to elution of the active ingredient, and it is used in excess of 300 parts by weight. In this case, the active ingredient may be diluted and the effect may be reduced.

As described above, the perilla leaf powder mixed with water may be extracted by heating at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 30 to 80 minutes. That is, it is preferable that the perilla leaf extraction is performed under pressure. This is to reduce the amount of heat used while maximally eluting the active ingredient of the perilla leaf, and it is possible to extract with the same efficiency with only 1/2 to 1/3 of the heating time compared to extraction by heating at normal pressure. In addition, when the extraction pressure is less than 100 kPa or when heated to less than 100 ° C, the extraction is not smooth. .

After the extraction is completed as described above, the perilla leaf extract can be prepared by removing the solids. The perilla leaf extract may contain 5 to 10 parts by weight based on 100 parts by weight of water in the hair loss prevention composition. When the perilla leaf extract is included in an amount of less than 5 parts by weight, it is difficult to expect the effect of the perilla leaf extract, and when it is included in an amount exceeding 10 parts by weight, the characteristic flavor of the perilla leaf extract becomes stronger, so the preference may be lowered when used.

The black bean is a general term for legumes having a black appearance such as heuktae, seoritae, and seomoktae, and contains a large amount of anthocyanins and flavonoids, so it has a great effect on improving blood circulation and preventing aging. In addition, it has a high content of isoflavones called phytoestrogens, which can help smooth the skin and relieve menopausal syndrome and osteoporosis. In relation to the present invention, it is known to help improve hair loss by containing the flavonoids and vitamins B1 and B12.

The black beans used in the present invention correspond to beans, unlike the woody trees (akashi tree branches), herbaceous plants (perilla leaf, hornwort), or flowers (soybean violet, akashi flower) used above. can be extracted.

To this end, the black bean extract is washed with black beans, followed by soaking in water for 60 to 120 minutes; mixing with 80 to 150 parts by weight of water based on 100 parts by weight of the soaked black bean, then heating at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 10 to 30 minutes; After the heating is completed, pressing the black beans; And after the compression is completed, it may be prepared by a method comprising the step of separating the black bean extract and the solid content.

The black beans can be washed and then soaked in water and soaked for 60 to 120 minutes. Common black coles are dried and sold after harvesting. These dried beans may be advantageous for storage, but have a disadvantage in that it is difficult to penetrate moisture during extraction as in the present invention. Therefore, it is preferable to use the black beans immersed in water after being washed. At this time, if the black beans are immersed for less than 60 minutes, extraction of the active ingredients may be difficult because the black beans are not sufficiently soaked. Ingredient content may drop.

Black beans that have been soaked as described above may be mixed with 80 to 150 parts by weight of water compared to 100 parts by weight of black beans, and then heated at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 10 to 30 minutes. In the water used in the soaking process, foreign substances on the surface of black beans may be removed. Therefore, after removing the water used in the soaking process, it is preferable to heat by mixing with 80 to 150 parts by weight of water based on 100 parts by weight of black beans. At this time, when the water is used in less than 80 parts by weight, sufficient water is not supplied, so the extraction amount of the active ingredient may be reduced, and when water exceeding 150 parts by weight is mixed, the content of the active ingredient may fall.

Black beans mixed with water as described above are preferably heated for 10 to 30 minutes at a pressure of 100 to 300 kPa and a temperature of 100 to 150 ℃. That is, the black bean can be extracted as an active ingredient through a pressurized extraction process. This is to reduce the amount of heat used while maximally eluting the active ingredient of the black bean, and it is possible to extract with the same efficiency with only 1/2 to 1/3 of the heating time compared to extraction by heating at normal pressure. In addition, the extraction is not smooth when the pressure is less than 100 kPa or when heated for a time of less than 100 ° C or less than 10 minutes during the extraction, and the extraction is performed at a pressure exceeding 300 kPa, a temperature exceeding 150 ° C, or for a time exceeding 30 minutes. In this case, it is inefficient because the amount of energy used during extraction increases.

After the pressure extraction is completed as described above, the black beans may be compressed. In the case of perilla leaf using a similar extraction process, since it has a thin leaf shape, most of the active ingredients can be extracted and eluted in the water. However, in the case of the black bean, since it has a bean shape, a significant active ingredient may exist in the bean even after the pressurized extraction as described above. In order to additionally extract this, the black beans are preferably compressed after the pressure extraction is completed.

After the compression of the black beans is completed as described above, the black bean extract can be prepared by separating the solids. The black bean extract may contain 10 to 15 parts by weight based on 100 parts by weight of water in the anti-hair loss composition. When the black bean extract is included in an amount of less than 10 parts by weight, the effect of preventing hair loss by the black soybean extract may be reduced. Side effects may occur.

The hair loss prevention composition can maximize the components and effects through a specific fermentation process after mixing the acacia tree extract, eoseongcho extract, soybean violet extract, perilla leaf extract and black bean extract.

To this end, the hair loss prevention composition comprises the steps of preparing an extract mixture by mixing an acacia tree extract, eoseongcho extract, soybean violet extract, perilla leaf extract and black bean extract prepared by the above method in water; mixing 1 to 5 parts by weight of an oligosaccharide with respect to 100 parts by weight of the extract mixture; Inoculating the lactic acid bacteria in the extract mixture in which the oligosaccharide is mixed, and culturing for 1 to 10 days at a temperature of 20 ~ 30 ℃; mixing 1 to 5 parts by weight of ground hops in the extract mixture solution in which the lactic acid bacteria culture is completed; Inoculating brewer's yeast into the extract mixture in which the ground hops are mixed, opening the lid, and then culturing the primary culture for 5 to 10 days at a temperature of 5 to 30 ℃; After the primary culture is completed, sealing and secondary culturing for 3-5 days at a temperature of 5-30 °C; and sterilizing the extract mixture at a temperature of 50 to 70° C. for 10 to 30 minutes after the secondary culture is completed.

The acacia tree extract, eoseongcho extract, soybean flower extract, perilla leaf extract and black bean extract prepared by the above method may be mixed in a certain ratio as described above. That is, based on 100 parts by weight of water, 10-15 parts by weight of acacia tree extract, 5-10 parts by weight of Eoseongcho extract, 5-10 parts by weight of bean violet extract, 5-10 parts by weight of perilla leaf extract and 10-15 parts by weight of black bean extract It is preferable to prepare an extract mixture by mixing.

1 to 5 parts by weight of oligosaccharides may be mixed with 100 parts by weight of the extract mixture in the extract mixture prepared as described above. The oligosaccharide is a material serving as food for lactic acid bacteria, which will be described later. In the present invention, some sugar is used for extraction, but in the case of preparing an extract mixture by mixing in the above ratio, the amount for culturing lactic acid bacteria may be insufficient. Therefore, it is preferable to mix the oligosaccharides to create an environment suitable for culturing lactic acid bacteria. At this time, when the oligosaccharide is included in less than 1 part by weight, it cannot have a sugar concentration for culturing lactic acid bacteria, and when it is added in excess of 5 parts by weight, stickiness may occur during use due to excessive oligosaccharides.

Mammary gland bacteria may be inoculated and cultured in the extract mixture mixed with the oligosaccharide as described above. In this case, the lactic acid bacteria used may be used without limitation as long as they can be cultured in the extract mixture, but preferably Lactobacillus plantarum, Lactobacillus sacai, Lactobacillus reuteri, Lactobacillus rhamnosus or a mixture thereof may be used.

The extract mixture inoculated with lactic acid bacteria as described above may be cultured for 1 to 10 days at a temperature of 20 to 30 ℃. At this time, if the extract mixture is cultured at less than 20 ° C or cultured for a period of less than 1 day, the culture of the lactic acid bacteria may not be smooth. Off-flavor due to growth may occur.

After the culturing of the lactic acid bacteria is completed as described above, 1 to 5 parts by weight of the ground hops may be mixed with the extract mixture. The hops may be used as a component to assist the growth of brewer's yeast, which will be described later, as a component added to produce the bitter taste and flavor of beer. At this time, when the hop pulverized product is included in less than 1 part by weight, the growth of brewing yeast is not smooth, and when it is included in excess of 5 parts by weight, off-flavor may occur due to overgrowth of yeast.

After the brewer's yeast is inoculated into the extract mixture to which the hops are added, the lid is opened, and then the primary culture may be performed for 5 to 10 days at a temperature of 5 to 30 ° C.

The brewer's yeast refers to yeast used to ferment beer, and the hair improvement effect due to this brewer's yeast has been recently reported. However, in the case of existing brewer's yeast, yeast generated during the fermentation of beer is used, so when mixed with other ingredients, its activity is mostly decreased. Most of them. In the case of the present invention, since white liquorice yeast is cultured using the extract mixture as described above, the activity of brewer's yeast does not fall when used, and thus the hair improvement effect of the brewer's yeast can be maximized.

The primary culture is a step of opening the lid and culturing, and at the same time proliferating the brewer's yeast and performing fermentation using brewer's yeast. In this step, the inoculated brewer's yeast can be multiplied by tens to tens of thousands of times, and the residual sugar and oligosaccharides can be converted into alcohol due to the activity of the brewer's yeast. At this time, if the primary culture is carried out at a temperature of less than 5 ℃ or is carried out for less than 5 days, the growth of brewer's yeast is not smooth and the hair improvement effect may be reduced, and when cultured at a temperature of 30 ℃ or more or for 10 days or more, off-flavor may occur.

After the primary culture is completed, the culture vessel may be sealed and secondary cultured for 3 to 5 days at a temperature of 5 to 30°C. In the case of the secondary culture, a brewer's yeast metabolite is produced using the brewer's yeast grown in the first culture. In general, in the case of beer, carbonic acid is produced in this step. Although carbonic acid is not required in the present invention, since the brewer's yeast and metabolites of brewer's yeast are utilized, it is preferable to produce the metabolites of the brewer's yeast through this step. At this time, if the temperature is less than 5 ℃ or secondary culture for less than 3 days, the generation of the metabolite may be reduced, and when the temperature exceeds 30 ℃ or cultured for more than 5 days, off-flavor may occur.

After the secondary culture is completed, it is preferable to sterilize the extract mixture at a temperature of 50 to 70° C. for 10 to 30 minutes. Generally used sterilization methods include a high-temperature sterilization method and a low-temperature sterilization method. In the present invention, it is preferable to use the pasteurization method in order to prevent thermal deformation of the extract. At this time, when the sterilization temperature is less than 50 ℃ or when sterilizing for less than 10 minutes, it is not completely sterilized, and the lactic acid bacteria and white yeast yeast can continuously proliferate and cause odor, and the temperature exceeds 70 ℃ or exceeds 30 minutes In the case of sterilization, the active ingredient of the extract may be thermally deformed, and thus the effect of preventing hair loss may be reduced.

The hair loss prevention composition may further include an emulsifier, a sunscreen, a moisturizer, a preservative, a pH adjuster, a thickener or other additives. As the hair loss prevention composition, the extract mixture prepared as described above may be used as it is, but in order to increase the marketability, lotion, cream, serum, sunscreen, makeup primer, makeup base, foundation, concealer, BB cream, CC cream, shampoo, It can be manufactured and sold in the form of soap, hair dye, perm, wax or spray. In particular, in the case of the anti-hair loss composition of the present invention, it is preferable to apply it directly to the scalp where hair grows. It is preferable to manufacture to have such a formulation.

Hereinafter, preferred embodiments of the present invention will be described so that those of ordinary skill in the art can easily implement them with reference to the accompanying drawings. In addition, in the description of the present invention, if it is determined that a detailed description of a related known function or a known concept may unnecessarily obscure the gist of the present invention, the detailed description thereof will be omitted. In addition, certain features presented in the drawings are enlarged, reduced, or simplified for ease of description, and the drawings and components thereof are not necessarily drawn to scale. However, those skilled in the art will readily appreciate these details.

Example 1

(1) Preparation of acacia tree extract

10 kg of acacia tree branches were sieved, washed with water, and crushed to a size of 3 cm. The pulverized acacia tree branch was charged into the expander, and then expanded at a temperature of 250° C. and a pressure of 1.5 Mpa.

After washing 10 kg of acacia leaves, dried in a dryer and pulverized to prepare dry akashi leaf powder.

After mixing 10 kg of akashi flower with the same amount of sugar, it was charged into a fermentation vessel and extracted at a temperature of 15° C. for 40 days to prepare an akashi flower extract.

After mixing 10 kg of the dried acacia leaf powder with 10 kg of the swollen acacia tree branch, it was charged into an extraction container and high-pressure extraction was performed by supplying water vapor at 600° C. for 5 hours. At this time, the pressure inside the extraction vessel was maintained at 300 kPa.

An acacia tree extract was prepared by mixing 3 kg of the acacia flower extract with 10 kg of the extract on which the high-pressure extraction was completed.

(2) Preparation of eoseongcho extract

The roots of the harvested Eoseongcho were removed and then washed with water.

20 kg of water was injected into 10 kg of the washed eoseongcho, and the ultrasonic wave of 65 kHz was supplied at an intensity of 700 W for 10 minutes to decompose the cellulose.

After the decomposition of the cellulose was completed, extraction was performed by heating at a temperature of 100° C. for 100 minutes, and after heating was completed, the extract was prepared by removing the solids using a filter.

(3) Preparation of Soybean Violet Extract

After mixing the same amount of sugar with 10 kg of soybean violet, it was charged into a fermentation vessel and extracted at a temperature of 25° C. for 50 days. After the extraction was completed, the solid content was removed to prepare a soybean violet extract.

(4) Preparation of perilla leaf extract

10 kg of sieved perilla leaves were washed with water, charged in a dryer, dried to a moisture content of 4% by weight, and powdered.

After mixing 12 kg of water with 5 kg of the powdered perilla leaf, extraction was performed by heating at a pressure of 200 kPa and a temperature of 120° C. for 60 minutes. After heating was completed, the solid content was separated using a filter to prepare a perilla leaf extract.

(5) Preparation of black bean extract

After washing 10 kg of black beans in water, immersed in water and soaked for 100 minutes. 10 kg of black beans soaked in water was mixed with 10 kg of water, and extracted by heating at a pressure of 250 kPa and a temperature of 140° C. for 20 minutes.

After the heating was completed, the active ingredient was extracted by pressing black beans, and black bean extract was prepared by mixing the heated extract and the liquid extracted by pressing.

(6) Lactobacillus culture

In 10 kg of water, 1.2 kg of the acacia tree extract of the step (1), 0.8 kg of the eosinus extract of the step (2), 0.7 kg of the bean violet extract of the step (3), and 0.6 kg of the perilla leaf extract of the step (4) And 1.3 kg of the black bean extract of step (5) was mixed to prepare an extract mixture.

After mixing 0.3 kg of oligosaccharide with 10 kg of the extract mixture, Lactobacillus plantarum, Lactobacillus sacki, Lactobacillus reuteri and Lactobacillus rhamnosus were inoculated with mixed lactic acid bacteria in equal amounts and cultured at a temperature of 25° C. for 7 days. did.

(7) Brewer's yeast culture

0.3 kg of ground hops was mixed with 10 kg of the extract mixture solution in which the culture of the lactic acid bacteria was completed, and brewer's yeast was inoculated. After the inoculation of the brewer's yeast was completed, the vessel was first cultured for 7 days at a temperature of 15°C in an open state, and after the primary culture was completed, it was sealed and secondary cultured at a temperature of 20°C for 4 days.

(8) Sterilization and composition preparation

After culturing the brewer's yeast was completed, it was sterilized at a temperature of 65° C. for 20 minutes, and then the solid content was separated using a filter to prepare a hair loss prevention composition.

Example 2

In Example 1, the akashi tree branch, akashi tree leaf, akashi tree flower, eosincho, soybean violet, perilla leaf and black bean were all mixed, mixed with water and extracted at a temperature of 100° C. for 60 minutes, except that The same was carried out.

Example 3

In Example 1, the same procedure was carried out except that no akashi tree branch, akashi tree leaf, and akashi tree flower were used.

Example 4

It was carried out in the same manner as in Example 1, except that the eoseongcho extract was not used.

Example 5

In Example 1, the same procedure was performed except that the soybean violet flower extract was not used.

Example 6

The same procedure was carried out in Example 1, except that perilla leaf extract was not used.

Example 7

It was carried out in the same manner as in Example 1, except that black soybean extract was not used.

Example 8

In Example 1, (6) was carried out in the same manner except that the lactic acid bacteria culture step was not carried out.

Example 9

In Example 1, (6) lactic acid bacteria culture step was not carried out, but 0.2 kg of the same commercially available lactic acid bacteria was mixed and used in the same manner.

Example 10

In Example 1, (7) was performed in the same manner except that the brewer's yeast culture step was not carried out.

Example 11

The same procedure was performed except that 0.5 kg of commercially available brewer's yeast was mixed instead of not performing the step (7) incubating brewer's yeast in Example 1 above.

Comparative Example 1

Minoxidil was used instead of the compositions of Examples 1 to 11.

Experimental Example 1

100 units/ml penicillin-100 μg/ml streptomycin (Gibco Inc, NY, USA) and 10% heat-inactivated fetal bovine (Rat vibrissa immortalized dermal papilla cell: Filsell et al., 1994) isolated from rat whiskers Using DMEM (Hyclone Inc, USA) medium containing serum (FBS; Gibco Inc, NY, USA), it _{was cultured in an incubator at 37°C and 5% CO 2} , and subcultured once every 3 days. The proliferation of dermal papilla cells was measured using the WST assay. ^{After turbidity of 1.0×10 4} cells/ml of dermal papilla cells in DMEM medium containing 1% FBS, put it in a 96 well plate, and incubate for 24 hours, the composition of Examples 1 to 11 and Comparative Example 1 0.1 μg, 1 μg, 10 μg and 100 μg were injected into each well. As a control, the same amount of water was injected and used. After the injection was completed, the well plate was incubated for 2 days, and then 10 μL of WST dye (Daeil Lab Service, Seoul, Korea) was added and reacted for 2 hours. After removing the supernatant and dissolving the precipitate by adding 200 μl of DMSO, the absorbance was measured at 450 nm using a VERSAmax microplate reader (Molecular Devices, CA, USA). The average absorbance value for each sample group was obtained, and the degree of proliferation was investigated by comparing it with the absorbance value (100%) of the control group.

All measurement results were expressed as mean ± standard deviation or mean ± standard error, and statistical significance was tested by the student's t test, and significance was recognized when the p-value was less than 0.05. Statistical processing was performed using Sigma Stat software (Jandel Scientific Software, USA).

0.1 μg 1 μg 10 μg 100 μg control 100% 100% 100% 100% Example 1 116.4% 119.8% 110.1% 96.4% Example 2 109.8% 111.1% 106.6% 94.3% Example 3 112.1% 115.4% 108.9% 96.7% Example 4 111.6% 114.8% 109.7% 94.3% Example 5 111.8% 113.9% 108.1% 95.9% Example 6 110.1% 112.9% 107.3% 96.8% Example 7 109.8% 110.9% 109.8% 95.1% Example 8 107.6% 111.1% 105.3% 91.4% Example 9 109.1% 112.2% 106.8% 94.8% Example 10 108.6% 111.8% 106.9% 95.4% Example 11 111.4% 114.1% 106.8% 96.1% Comparative Example 1 110.2% 112.5% 108.1% 95.8%

As shown in Table 1, in the case of Example 1 of the present invention, compared to Comparative Example 1 using minoxidil, it was found that the dermal papilla cell proliferation effect was superior. However, in the case of Example 2, in which all components were extracted at the same time, although it had a proliferation effect of dermal papilla cells, it was shown to have a lower effect than Example 1 of the present invention and Comparative Example 1 using minoxidil, Example 1 of the present invention As shown, it was confirmed that the use of an appropriate extraction method for each component has a large effect of hair bunting.

In the case of Examples 3-7 except for some components, it was shown to have a lower effect compared to Example 1, and in Example 8 without culturing mammary gland bacteria and Example 10 without culturing brewer's yeast, compared to Example 1 appeared to have a low effect. In addition, in the case of Example 9 in which the purchased lactic acid bacteria were simply mixed and Example 11 in which the purchased brewer's yeast was mixed, the proliferation of papillary cells was compared to Example 8 in which the lactic acid bacteria were not cultured and Example 1 in which the brewer's yeast was not cultured. Although the effect was found to be high, it was confirmed that the activity was lower than that of the present invention because it was shown to have a lower effect than that of Example 1.

In the above, preferred embodiments of the present invention have been illustrated and described, but the present invention is not limited to the specific embodiments described above, and it is common in the technical field to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. Various modifications are possible by those having the knowledge of, of course, these modifications should not be individually understood from the technical spirit or prospect of the present invention.

Claims (2)

In the anti-hair loss composition comprising an acacia tree extract, eoseongcho extract and soybean violet extract,
The hair loss prevention composition,
Contains 10-15 parts by weight of acacia tree extract, 5-10 parts by weight of eosinthia extract, 5-10 parts by weight of soybean extract, 5-10 parts by weight of perilla leaf extract and 10-15 parts by weight of black bean extract relative to 100 parts by weight of water and
The acacia tree extract,
crushing the acacia tree branches to a size of 1-5 cm;
swelling the pulverized acacia tree branches at a temperature of 100 to 300° C. and a pressure of 1 to 2 MPa;
mixing 50 to 200 parts by weight of dry powder of acacia leaves based on 100 parts by weight of the swollen acacia tree branch;
inserting a mixture of the swollen acacia tree branch and dry powder of acacia tree leaves into an extraction container, and then supplying water vapor at 500 to 800° C. for 3 to 10 hours for high pressure extraction; and
preparing an acacia tree extract by mixing 30-50 parts by weight of an acacia flower extract with 100 parts by weight of the extract generated in the high-pressure extraction step;
It is prepared by a method comprising
The akashi flower extract,
Mixing 50 to 150 parts by weight of sugar compared to 100 parts by weight of akashi flower;
charging the akashi flowers mixed with the sugar into a fermentation vessel;
The step of sealing the fermentation vessel loaded with the akishi flower, and then extracting at a temperature of 10 to 20 ℃ for 30 to 50 days; and
After the extraction is completed, removing the solids to prepare an akashi flower extract;
It is prepared by a method comprising
The eoseongcho extract,
After removing the roots of the harvested Eoseongcho, washing;
Decomposing the cellulose of eoseongcho by injecting 100 to 300 parts by weight of water compared to 100 parts by weight of the washed eoseongcho and supplying ultrasonic waves having a frequency of 10 to 70kHz at an intensity of 500 to 1000W for 5 to 20 minutes;
heating the eoseongcho in which the cellulose is decomposed at a temperature of 90 to 110° C. for 10 to 120 minutes; and
Separating the solid content and Eoseongcho extract after the heating is completed;
It is prepared by a method comprising
The soybean violet flower extract,
mixing 50 to 150 parts by weight of sugar with respect to 100 parts by weight of soybean violet;
charging the soybean violet mixed with the sugar into a fermentation vessel;
The step of sealing the fermentation vessel in which the soybean violet is charged, and then extracting at a temperature of 20 to 30° C. for 40 to 60 days; and
After the extraction is completed, removing the solids to prepare a soybean violet extract;
It is prepared by a method comprising
The perilla leaf extract,
washing the collected perilla leaflets and then drying them to a moisture content of less than 5%;
pulverizing the dried perilla leaf to prepare perilla leaf powder;
mixing 200 to 300 parts by weight of water to 100 parts by weight of the perilla leaf powder, then heating the mixture at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 30 to 80 minutes; and
separating the perilla leaf extract and the solid content after the heating is completed;
It is prepared by a method comprising
The black bean extract,
After washing black beans, soaking in water for 60 to 120 minutes;
mixing with 80 to 150 parts by weight of water based on 100 parts by weight of the soaked black bean, then heating at a pressure of 100 to 300 kPa and a temperature of 100 to 150° C. for 10 to 30 minutes;
After the heating is completed, pressing the black beans; and
After the compression is completed, separating the black bean extract and the solid content;
It is prepared by a method comprising
The hair loss prevention composition,
Preparing an extract mixture by mixing the acacia tree extract, eoseongcho extract, soybean violet flower extract, perilla leaf extract and black bean extract prepared by the above method in water;
mixing 1 to 5 parts by weight of an oligosaccharide with respect to 100 parts by weight of the extract mixture;
Inoculating the lactic acid bacteria in the extract mixture in which the oligosaccharide is mixed, and culturing for 1 to 10 days at a temperature of 20 ~ 30 ℃;
mixing 1 to 5 parts by weight of ground hops in the extract mixture solution in which the lactic acid bacteria culture is completed;
Inoculating brewer's yeast into the extract mixture in which the hops are mixed, opening the lid, and then culturing the primary culture for 5 to 10 days at a temperature of 5 to 30 ℃;
After the primary culture is completed, sealing and secondary culturing for 3-5 days at a temperature of 5-30 °C; and
sterilizing the extract mixture at a temperature of 50 to 70° C. for 10 to 30 minutes after the secondary culture is completed;
It is prepared by a method comprising
The hair loss prevention composition further includes an emulsifier, a sunscreen, a moisturizer, a preservative, a pH adjuster or a thickener, and a lotion, cream, serum, sunscreen, makeup primer, makeup base, foundation, concealer, BB cream, CC cream, shampoo, A composition for preventing hair loss, characterized in that it is in the form of soap, hair dye, perm, wax or spray.
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