KR102269337B1 - Hyperthermophile fermentation treatment system in the affected aera of foot and mouth disease - Google Patents

Abstract

The present invention relates to a livestock liquid feces treatment system (1). The livestock liquid feces treatment system (1) ferments and decomposes livestock liquid feces by hyperthermophiles and includes: a first process (3) of moving to an outbreak site, excavating a burial site (10), and treating livestock liquid feces by hyperthermophiles; a second process (6) of moving the livestock liquid feces to a base treatment facility (7) to perform secondary treatment after the first process; and a composting process of composting the results of first and second processes.

Description

Hyperthermophile fermentation treatment system in the affected aera of foot and mouth disease }

The present invention relates to a process for treating livestock feces infected in an area affected by foot-and-mouth disease, and more particularly, in fermenting livestock feces by ultra-high temperature microorganisms (YM bacteria), a method of burying pathogens that primarily kills pathogens in the field is applied. It relates to a technology that can improve the structure of the aeration unit and secondarily increase the processing efficiency of feces by transferring it to a base treatment facility for fermentation treatment.

In general, liquid manure is a mixture of livestock manure, urine, feed, straw, and cleaning water, and has a variety of moisture content. Usually, liquid manure is a liquid with a moisture content of 85% or more, which is anaerobic or aerobic by aeration or agitation. It means that the decomposition is completed and stabilized by ripening in

The form of such liquid manure can be separated into facultative anaerobic fertilization, anaerobic, aerobic fertilization, and liquid fertilization by additives according to the fertilization method.

In addition, this liquid manure is generated in large amounts every year, causing environmental pollution, etc., and the amount of the generation is as follows.

That is, as shown in the graph of FIG. 14 , except for a large decrease due to the influence of foot-and-mouth disease in 2011, the amount of livestock excreta generation tends to repeat a small increase and decrease. The amount of excrement largely depends on the number of pigs, such as pigs with a large daily amount of manure, and the amount of manure increased together in 2010, 2012, 2014, and 2016, when the number of pigs raised, and from 2007 to 2016, about 1,700 tons per day. /day, except for the decrease due to the effects of foot-and-mouth disease in 2011, it is maintained at a similar amount.

And, this liquid manure is being treated in various ways, an example will be described as follows.

That is, when liquid manure is separated from manure, urine and sewage are purified by physical, chemical, and biological treatment methods and then discharged, and the manure is reduced to soil through a composting process after controlling moisture.

On the other hand, when excreta is mixed, the mixed manure is stored in a liquid manure tank for more than 6 months, and after stabilizing, it is sprayed on the soil, or after solid-liquid separation, the manure is purified and recycled.

In this case, the biological treatment method that combines physical and chemical methods is applied as pre- and post-treatment for purification treatment. Recently, high-level treatment and high-efficiency treatment methods are being sought due to the strengthening of effluent quality standards.

In addition, livestock manure can be divided into liquid, semi-solid, and solid according to the ratio of solids, and requirements for handling are different according to their physical properties.

In general, the physical properties of livestock manure generated vary according to the amount of cleaning water and litter inside the barn. In general, up to 8-10% of the solid material content is considered liquid manure, so a solid transfer pump can be used. Solid or semi-solid livestock manure is collected with a tractor-type scraper or mechanical scraper and stored in a conventional storage tank. After stabilization, it can be applied to the land as compost.

However, if it is confirmed that serious damage is caused by the spread of diseases such as foot-and-mouth disease, such liquid excreta, livestock, excreta, people, vehicles, goods, etc., enter and exit from all cattle livestock farms and related workplaces in the country or in the area where it occurs. measures will be taken to temporarily suspend

This is defined as a standstill for livestock, etc., and the temporary suspension period can be extended within 48 hours from the point of occurrence depending on the test results.

In this case, the farmer shall process and store the livestock manure by making the most of the livestock excreta treatment facility and storage tank possessed by the farm. Therefore, it can be taken out after receiving confirmation from the mayor or governor.

In particular, compared to other livestock breeds, pigs emit a large amount of feces of about 5.1 kg per day, and about 53,000 tons of pig manure is generated nationwide.

Small farms do not have their own manure treatment facilities and rely on consignment treatment to process manure. In addition, as 33.8% of livestock manure is processed by consignment treatment nationwide, it is difficult to process the manure on its own in small-scale farmhouses that do not have a large excrement storage tank when a temporary stop is triggered.

Table 1 below shows the status of consignment processing by province.

On the other hand, after the temporary suspension of foot-and-mouth disease outbreak in the winter of 2011 was activated, livestock manure that could not be treated by itself was left on farmland or roads or was discarded.

Abandoned manure emits a foul odor, and there is a possibility that infectious agents such as foot-and-mouth disease can be spread by vehicles, people, and wind. Foot-and-mouth disease virus has the potential to survive up to 180 days in manure and can be spread by winds up to 50 km. This situation increases the possibility of accumulating livestock manure on farms in restricted areas, making it a major obstacle to quarantine of infection vectors.

More specifically, if the temporary suspension period is prolonged in a farmhouse in a hazardous area (within a radius of 500m) and exceeds the farmhouse's own processing capacity, storage facilities such as a simple storage tank may be secured, or landfills in national and public land within the movement restricted area. , and treat it with an acidic agent (hydrochloric acid, citric acid) or an alkali agent (caustic soda, quicklime) and then landfill to treat the manure.

And, farmhouses in the boundary area (within a radius of 500m to 10km) treat livestock manure using compost liquid manure and purification methods. In livestock excreta fermentation facilities and composting houses, manure should be put into the fermentation bed together with sawdust and rice husks to maintain the heat of fermentation at 60℃ or higher.

In the liquefaction process, the manure should be completely ripened and sprayed on farmland in compliance with pH 6.0 or less or pH 11.0 or more, and the slurry generated during the liquefaction process is treated by aeration.

In addition, since the vitality of microorganisms in the aeration tank is lowered by disinfectants during biological purification treatment, an additional seeding agent is added, and the purified effluent must be discharged at a pH of 6.0 or less or 11.0 or more when discharged.

And, if it is unavoidable due to a lack of processing and storage facilities on the farm after movement restrictions have been issued, chemical disinfection treatment is followed. The current SOP chemical excreta treatment method uses alkaline agents such as caustic soda (NaOH) and quicklime to raise the pH to 10, then lowers it to pH 5 with an acidic agent such as citric acid to kill the virus through a neutralization process. Mix 1 ton (1000L) of liquid manure with 5 kg of 98% pure caustic soda (NaOH) and leave it in isolation for 3 days or more, or mix 11.0 kg of 85% pure quicklime (CaO) and leave it in isolation for 7 days or more. Neutralization is carried out so that the pH is 6-8 using the agent.

However, the chemical excreta disinfection treatment method requires a stirring process of mixing the manure with a disinfectant such as caustic soda, quicklime, citric acid, etc., but it is difficult to stir the manure and disinfectant in the farmhouse, and the surrounding environment is polluted by the leachate generated by the disinfectant and the manure. There is a problem of

In addition, livestock excreta is sometimes treated using methods such as composting, liquid composting, and purification and discharge in excreta treatment facilities such as public treatment facilities. When sterilizing excrement with a chemical disinfectant, the activity of microorganisms that promote the composting process is likely to decrease. In addition, the number of microorganisms required during the decomposition process by aeration of the slurry generated during the liquid composting process can be reduced. In addition, active sludge, a group of microorganisms that oxidatively absorb and decompose necessary organic matter during the purification process, may die.

On the other hand, the main reason for the failure of the initial quarantine in case of national catastrophic epidemics including foot-and-mouth disease is that a large amount of foot-and-mouth disease virus is emitted from animals including pigs through manure. Because disease is transmitted through

In particular, since the amount of liquid manure discharged from pig farms is very large, it is a reality that there are insufficient methods to effectively treat liquid manure along with carcass treatment. It is difficult to supply and demand burial sites burying a large amount of liquid manure in pig farms, and when burial cannot be carried out any longer, liquid manure transport vehicles are used to take them out.

In addition, the method of chemically treating a large amount of liquid manure may cause environmental pollution, and there is a disadvantage that foot-and-mouth disease virus and pathogenic microorganisms present in organic matter are not properly killed.

In addition, the method of killing pathogenic microorganisms including foot-and-mouth disease virus by artificially applying heat to a large amount of liquid manure may have practical difficulties such as expensive treatment cost and development of large-scale precision treatment equipment.

And, in general, liquid manure treatment is returned to farmland and rivers through composting, liquid manure, and purification and discharge, but excessive composting and liquid manure can cause problems such as spraying exceeding the environmental capacity of farmland, so it is effective in terms of safety and economy. can't see

In addition, chemical disinfectants (caustic soda, quicklime, citric acid) used in the past to kill the foot-and-mouth disease virus take more than 4 days due to the process of alkalizing and neutralizing again by raising the pH to 10, composting, purification and discharge process and feces There is a problem in that it kills microorganisms and activated sludge required for the excreta purification process in excreta treatment facilities such as public treatment plants.

Patent Application No. 10-2015-119030 (Name: Eco-friendly method of killing animals)

Therefore, the present invention has been proposed to solve this problem, and an object of the present invention is to directly ferment the liquid manure discharged from the farmhouse where foot-and-mouth disease has occurred by using the ultra-high temperature microflora, without taking it out to the outside, but by fermenting it at a high temperature. To provide a technology capable of killing existing foot-and-mouth disease viruses and other pathogenic microorganisms.

Another object of the present invention is to ferment livestock feces by ultra-high temperature microorganisms (YM bacteria), primarily by applying a method of burial in the field, and secondly by transferring them to a base treatment facility for fermentation treatment. It is to provide a technology that can further increase processing efficiency.

In addition, another object of the present invention is that it is possible to decompose livestock feces infected with highly pathogenic AI and foot-and-mouth disease virus in a short time, as well as to follow-up the final fermented product, due to the excellent effect of killing pathogens at an ultra-high fermentation temperature (85 ~ 110 ℃). It is to provide an eco-friendly and sustainable livestock feces treatment technology that is 100% recyclable for the fecal treatment of livestock liquid in the fermentation process.

An embodiment of the present invention in order to achieve the above technical problem,

To provide a livestock liquid feces treatment process for fermenting and decomposing livestock liquid feces by ultra-high temperature microorganisms,

The livestock liquid feces treatment process includes: a first process (3) including a buried fermentation process (3) of excavating a burial site (10) in an area adjacent to an outbreak site to treat liquid feces of livestock;

After the first process (3), it includes a second process (6) of moving the liquid feces of livestock to a base treatment facility (7) and treating it,

The buried fermentation process (3) of the first process (3) excavates the burial site 10 at the site of the outbreak, mixes livestock liquid feces and ultra-high temperature microbial medium into the burial site 10, and then excavates As a process of fermenting the burial site 10 back into the soil to restore it to the original soil before the onset of the disease,

An impermeable material (12) is placed on the bottom and sides of the burial site (10), and a diffuser section (16) is disposed thereon;

The diffuser 16 includes an inlet pipe 18 to which compressed air is supplied; a diffuser pipe 20 connected to the inlet pipe 18 and discharging air to the outside; a branch pipe 22 connecting between the diffusers 20 and discharging air; It includes an elastic member 24 that is disposed to surround the outside of the branch pipe 22 so that air effectively penetrates between the mixture of the livestock feces and the ultra-high temperature microbial medium by forming a gap when the livestock feces and the ultra-high temperature microbial medium are covered.

As described above, the ultra-high temperature fermentation processing system of livestock feces in an area affected by foot-and-mouth disease according to an embodiment of the present invention has the following advantages.

First, it is possible to kill the foot-and-mouth disease virus and other pathogenic microorganisms present in the excrement by directly high-temperature fermentation in the farmhouse without taking out the liquid excreta discharged from the farmhouse where foot-and-mouth disease has occurred by using the ultra-high temperature microbiota.

Second, in fermenting livestock feces in the foot-and-mouth disease outbreak area with ultra-high temperature microorganisms (YM bacteria), firstly, the method of burial on site is applied, and secondly, it is transferred to a base treatment facility and fermented by The processing efficiency can be further improved.

Third, when livestock excrement is processed in the field by a buried method, it can be fermented more efficiently by improving the structure of the aeration organ that supplies air.

Fourth, it is a method of efficiently processing liquid manure using ultra-high temperature aerobic microorganisms. Since manure is mostly composed of organic substances and water, it is possible to treat ultra-high temperature aerobic microorganisms. As an eco-friendly method to reduce organic waste by lifting, the manure is completely decomposed in one week, and foot-and-mouth disease virus and other pathogenic microorganisms present in the liquid manure of pigs treated under high-temperature fermentation conditions of about 100°C can be completely killed.

Fifth, safety standards will be established by presenting the result of the complete annihilation of pathogens including foot-and-mouth disease virus from the residues of manure treated with ultra-high temperature microflora, and a cycle of resource utilization can be formed by composting the treated waste.

Sixth, it is thought that it is possible to prevent contamination of forests, rivers, and groundwater due to the decay of liquid feces buried for decades, and to greatly reduce social costs due to burial by reducing reprocessing costs.

1 is a diagram schematically showing the overall configuration of an ultra-high temperature fermentation processing system for livestock feces in an area affected by foot-and-mouth disease according to an embodiment of the present invention.
FIG. 2 is a view showing the buried type ultra-high temperature liquid fecal treatment process shown in FIG. 1 .
3 is a side view showing the structure of the buried feces treatment process shown in FIG. 2 .
4 is a perspective view showing the structure of the diffuser shown in FIG. 2 .
5 is a partial enlarged view showing an enlarged portion "A" of FIG.
6 is a perspective view schematically showing the external structure of the base treatment facility shown in FIG. 1 .
7 is a perspective view illustrating an internal structure of FIG. 7 ;
FIG. 8 is a diagram schematically illustrating a structure in which first and second temperature sensors and a control unit are applied to the ultra-high temperature fermentation processing system of livestock feces in the foot-and-mouth disease outbreak region shown in FIG. 1 to proceed according to the fermentation temperature of each process.
9 is a view showing a state in which livestock liquid manure is supplied to the base treatment facility shown in FIG.
FIG. 10 is a view showing the stirring state of the fermented product in the base treatment facility shown in FIG. 6 .
11 is a view showing a state in which livestock feces are completely processed in the base treatment facility shown in FIG. 6 .
12 is a view showing the pathogen killing experimental process in the ultra-high temperature aerobic fermentation system.
13 is a diagram showing a TCID process for identifying residual viruses.
14 is a graph showing the number of breeding heads and the amount of manure generated by year.
15 is a schematic diagram of ultra-high temperature microbial preparations and liquid manure mixing and fermentation process.
16a and 16b are graphs showing the fermentation temperature during on-site burial treatment of pig liquid manure.
17 is a fertilizer specification test report for compost, a result of treatment of livestock liquid manure according to an embodiment of the present invention.

Hereinafter, an ultra-high temperature fermentation processing system for livestock feces in an area affected by foot-and-mouth disease according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

As shown in Figures 1 to 15, the livestock liquid fecal treatment process (1) proposed by the present invention is a bar treated over two times: the primary treatment process (3) and the secondary treatment process (6),

In the primary treatment process (3), the burial site is excavated in an area adjacent to the site of foot-and-mouth disease or AI outbreak and treated by the buried process (3) of processing the liquid feces of livestock.

Then, in the secondary treatment step (6), the liquid feces that have been primarily treated are moved to the base treatment facility (7) to perform secondary treatment.

If we describe this livestock liquid fecal treatment system in more detail,

The burial fermentation process, which is the primary treatment process (3), excavates a burial site 10 at the site of the outbreak, mixes livestock liquid feces and ultra-high temperature microbial medium into the burial site 10, and puts it into the excavated soil. It is a method of fermenting by covering the buried land 10 again.

As shown in Figures 2 and 3, this burial site 10 can be excavated to various depths, but preferably does not exceed 3m, and the scale per place is 300m3 (3m*5m*20m) excavated with At this time, the distance between the burial sites 10 is preferably 6m or more so that the movement of people and equipment is easy.

Then, an impermeable material 12 such as vinyl is disposed on the bottom and side of the buried paper 10, and a nonwoven fabric or a vinyl cover 14 is additionally covered thereon to prevent damage to the vinyl. However, when a high-strength waterproof material such as high-density polyethylene (HDPE) is used instead of vinyl, additional covering of non-woven fabric, vinyl cover, etc. may be omitted.

In this case, an environmentally friendly product is recommended for vinyl, and it is used in a size larger than the volume of the burial paper 10. (Double vinyl with a thickness of 0.2 mm or more, high-strength waterproof material)

In the conventional liquid fecal treatment facility 7, an internal leachate storage tank and a leachate discharge pipe (perforated pipe: an opening/closing device at the top) should be installed at the bottom of the burial site 10, but this can be omitted in the buried process of the present invention.

And, the bottom of the burial ground is provided with a diffuser 16, so that fermentation of livestock feces can be promoted.

That is, as shown in FIGS. 4 and 5, the diffuser 16 includes an inlet pipe 18 to which compressed air is supplied; a diffuser pipe 20 connected to the inlet pipe 18 and discharging air to the outside; a branch pipe 22 connecting between the diffusers 20 and discharging air; It includes an elastic member 24 that is disposed to surround the outside of the branch pipe 22 so that air effectively penetrates between the mixture of the livestock feces and the ultra-high temperature microbial medium by forming a gap when the livestock feces and the ultra-high temperature microbial medium are covered.

In the diffuser unit 16 having such a structure,

The air diffuser 20 may be arranged in various shapes on the bottom of the burial site, for example, may be arranged in a rectangular shape, a zigzag shape, etc., which may be appropriately selected according to the environment of the burial site.

In addition, the branch pipe 22 connects the diffuser pipe 20 and the diffuser pipe 20 so that the air supplied through the diffuser pipe 20 can be supplied to the branch pipe 22 as well. Also, the branch pipe 22 is connected to the diffuser pipe 20 through a connecting pipe 26 .

At this time, the air may be discharged to the outside by forming a plurality of air outlets in the air diffuser 20 and the branch pipe 22 .

And, the air discharged to the outside is fermented by penetrating between the livestock feces and the medium of ultra-high temperature microorganisms.

At this time, the elastic member 24 disposed to surround the outside of the branch pipe 22 allows air to better penetrate into the gap between livestock feces and the medium of ultra-high temperature microorganisms.

That is, the elastic member 24 has a coil shape like a spring, and is disposed to surround the outside of the branch pipe 22 . At this time, when the distance between the coils is densely formed, when livestock excrement and medium are poured and buried in a state disposed on the bottom of the burial site, voids are formed around the outer peripheral surface of the branch pipe 22, so that air is effectively dispersed throughout the voids. can

Therefore, livestock feces and media can be fermented more effectively by the burial method in the field.

At this time, the height of the burial site is appropriate to be 2 m high, and if necessary, it is covered with an ultra-high temperature microbial medium of 0.5 m or more. In this case, the process of sterilizing with separate quicklime or the like may be omitted. In addition, there is no need to install a gas discharge pipe and an external storage tank (simple tank) at the bottom of the burial site 10 .

In addition, the conventional facility periodically sprays a fermenting agent and a deodorant or aerobic, thermophilic microorganisms, etc. when buried in liquid feces to remove odors and additionally sprays them when the odor is severe, but the present invention provides such facilities due to the improved decomposition ability of ultra-high temperature microorganisms is not needed

After the liquid feces of livestock are primarily decomposed by the burial method, they are transferred to the base treatment facility (7) and the fermentation process (6) is secondarily performed, whereby complete decomposition can be achieved.

At this time, the fermentation treatment period in the base treatment facility 7 is appropriate for about 3 weeks. And, if necessary, the final decomposition product can be recycled again and 100% recycled as an ultra-high temperature fermentation medium.

On the other hand, the ultra-high temperature microorganism can decompose the liquid feces of livestock efficiently in a short period of time by having the following physical properties.

The ultra-high temperature microorganism used in the present invention is named Hyperthermophile in English, and is also called Hyperthermophile or Archaea in Korean.

Specifically, the ultra-high temperature microorganism of the present invention is an aerobic thermophilic group that operates and moves in an ultra-high temperature aerobic condition of 85 to 110 ° C., and hygienically and rapidly ferments and decomposes all organic wastes such as food waste, sewage sludge, livestock manure, and spirit sludge. Since the body composition of livestock liquid feces is mostly composed of protein and fat organic matter and water, it can be fermented and decomposed by treatment with ultra-high temperature aerobic microorganisms.

In the fermentation decomposition process, heat-resistant enzymes such as protease, collagenase, and lipase produced by ultra-high temperature microorganisms rapidly decompose hard-to-decompose proteins and lipids, which are hard-to-decompose organic substances that general aerobic microorganisms cannot decompose, such as keratin in livestock feces.

The decomposed organic matter is used for the energy of microorganisms, and in the process, heat is rapidly generated and the decomposition temperature is 85℃ or higher. This ultra-high temperature environment further increases the activity of decomposing enzymes, which promotes the decomposition of organic matter and accelerates the decomposition rate. Finally, animal liquid fecal body components are released as harmless and treatable gases such as carbon dioxide, water vapor, and ammonia.

The growth temperature of the ultra-high temperature microorganisms (YM bacteria) applied to the present invention is around 100 ° C, which is a big difference from the growth temperature of around 55 ° C of general aerobic fermentation bacteria. It has excellent weight loss rate and excellent killing effect on pathogenic bacteria and viruses. In particular, it is excellent in removing odors, reducing waste by more than 85%, and at the same time as a permanent resource circulation system that 100% of the final product is recycled, it is a liquid fecal treatment method that completely decomposes livestock feces within 10 days and has no secondary by-products.

As described above, the ultra-high temperature microorganism of the present invention is generally defined as having an optimal growth temperature of 85 to 110 ° C, inhabits at the boiling point of water or higher, and grows well even under high temperature-high pressure- high salt conditions, so 50 to 70 ° C. It is completely distinguished from general high-temperature microorganisms with the optimum growth temperature of , and completely decomposes animal and poultry liquid feces within 10 days.

The ultra-high temperature microorganism used in the present invention is a hyperthermophil belonging to the genus Caldothrix, which does not proliferate below 50° C. and can grow at 80° C. or higher. It preferably belongs to Caldothrix Satsumae, and most preferably belongs to Caldothrix Satsumae YM081 (FERM BP-8233) strain.

Details of the Cardothrix Satsumae YM081 (FERM BP-8233) are described in Korean Patent Registration No. 10-0702258. Specifically, a bacterium named Caldothrix satsumae, YM081 was deposited in the Patent Biology Deposit Center of the National Institute of Industrial Technology, an independent administrative agency, and an accession number FERM P-18598 was given. And after that, it transferred to the international deposit and was given an accession number FERM BP-8233.

The contents of the deposited biological material of the present invention are as follows.

1. The name of the depository institution that deposited the biological material and the name of the recipient

Name: Industrial Technology Research Institute Patent Biology Deposit Center

Address: ZIP Code 305-8565 Tsukuba-shi, Ibaraki Prefecture, Japan 1-chome 1, Central 6

2. Date of deposit in the institution of 1 : November 7, Heisei 14 (2002) (original deposit date: November 13, Heisei 13 (2001) )

3. Accession number issued for the deposit by the institution in 1: FERM BP-8233

The inventors of the present invention obtained the strain with the consent of the holder of the patent, devised a plan to apply these ultra-high temperature microorganisms to the fecal treatment of livestock liquid of the present invention, and were able to achieve considerable results as a result of testing in various ways.

As described above, the system for treating livestock liquid feces by ultra-high temperature aerobic microorganisms according to the present invention has the advantage of solving the problems of treating livestock liquid feces and effectively discharging the livestock liquid feces treatment system.

With the development of high-efficiency liquid fecal treatment technology using ultra-high temperature aerobic microorganisms, the problems of existing liquid fecal treatment such as environmental pollution due to organic matter and social loss due to long-term treatment period, which have been problematic, will be able to be improved.

AI and foot-and-mouth disease outbreaks have the advantage of designing a disinfection facility capable of killing and using it as a policy, and actively discussing it with each local government to create a type of disposal facility suitable for each region.

On the other hand, as described above, after the buried fermentation process (3), the secondary fermentation process (6) is performed in the base treatment facility (7), and the base treatment facility (7) is as shown in FIGS. 6 and 7 . Likewise, it is a system for treating livestock feces by ultra-high temperature fermented bacteria at 100°C or higher, and has a front open form in a partition type. This method has the advantage of simplifying the flipping process with minimal equipment and has a stackable system that maximizes air supply.

Such a system (1) can efficiently remove moisture evaporated into steam at a maximum fermentation temperature of 110° C. while appropriately controlling the amount of air required for fermentation in the fermentation tank. (Adjusting the amount of air injection about 10 to 30 L/m ² ㆍmin)

The purpose of the post-ripening facilities such as the base treatment facility 7 is to decompose and stabilize the undecomposed organic matter in the primary fermentation process, and at the same time to control various odors remaining in the fermented product or livestock liquid feces.

These base treatment facilities 7 include a plurality of fermenters 25; a mixing tank 27 for mixing livestock liquid feces fermented in the fermenter 25 and ultra-high temperature microbial medium; It includes a blower (Blower; 29) for blowing air.

More specifically, in the plurality of fermenters 25, as shown in FIG. 10, liquid feces and YM new medium of livestock are sufficiently covered in an acid form on the air pipe so that foreign substances and moisture do not block the air hole of the pipe.

Then, by operating the blower 29, it is checked visually and by touch whether the air is well discharged.

In this state, fermentation is performed in the fermenter 25, and after a certain period of time has elapsed, it is transferred to the mixing tank 27 using a payloader or the like.

At this time, the properties of the mixtures are confirmed, respectively. For example, moisture content, specific gravity, weight, odor, salt, and the like. And calculate the mixing amount with the YM medium according to the properties of each mixture, the mixing amount calculation standard is preferably about 40 to 45% moisture content.

Then, as shown in FIG. 10, the YM medium and the liquid feces of livestock are uniformly mixed and mixed with a payloader equipment until it is in an optimal state.

When sufficient mixing is achieved, the wastes mixed in the plurality of fermenters 25 are transferred and piled up.

And, check the properties of the waste accumulated in the fermenter 25 before the start of fermentation. For example, moisture content, specific gravity, weight, odor, salt, and the like.

In addition, the upper end of the fermenter 25 is divided into thirds in the vertical direction, and three thermometers are installed to a depth of 0.6 to 1 m avoiding the air pipe. And, it is preferable to measure and record the temperature three times a day until the end of the fermentation between the total fermenter 10 (40 to 45 days).

When the measurement preparation is completed, air is supplied to the fermenter 25 by operating the blower 29 . And, as the fermentation proceeds, the supply amount of air is appropriately adjusted, and the fermentation temperature is also grasped.

If the temperature of the fermentation waste rises to the maximum and then falls, it means that the stirring timing is imminent. In general, the stirring timing is 7 to 10 days when the temperature drops to the maximum.

Agitation is carried out with the payloader heavy equipment, and when stirring, the waste is sufficiently shaken off with the payloader to blow away moisture.

As such, when a certain period of time has elapsed in the fermenter 25, the organic wastes are moved to the neighboring fermenter 25 for secondary fermentation. Then, the moved wastes repeat the measurement and fermentation process, and after fermenting through the stirring process about 5 to 6 times, the fermentation is terminated (see FIG. 11).

This base treatment facility 7 is a direct final fermentation reaction, shortening the period of the primary fermentation process and rapidly stabilizing organic matter at an average reaction temperature of 100° C. or higher, so that reduction of organic waste and fermentation can proceed simultaneously.

In this way, the liquid fraction of livestock treated in the first and second stages can be recycled through the composting process.

On the other hand, in the livestock liquid feces treatment system of the present invention, the step by the buried fermentation process (3) is firstly performed, and secondly, the treatment step of the livestock liquid feces is performed in the base treatment facility (7), This livestock liquid fecal treatment method can be performed step by step by means of a temperature sensor.

That is, as shown in FIG. 8 , the first temperature sensor T1 is mounted on the buried paper 10 of the buried fermentation process 3 to measure the fermentation temperature.

Then, the temperature value measured by the first temperature sensor T1 is transmitted to the central control unit C through the network.

The control unit (C) can grasp the progress of the buried fermentation process (3) by sensing the temperature of the buried ground (10) by the first temperature sensor (T1).

In addition, the temperature value is transmitted to the control unit (C) from the second temperature sensor (T2) disposed in the fermenter (25) of the base treatment facility (7).

Therefore, the control unit (C) can grasp the progress of the buried fermentation process (3) that is primarily carried out by the first and second temperature sensors (T1, T2), and also the base treatment facility that is carried out secondarily The progress in (7) can be grasped.

And, after grasping the progress state in each process, it is possible to properly proceed by instructing whether to proceed with each process.

For example, when the fermentation temperature of the buried fermentation process (3), which is the primary process, reaches a temperature range of about 85 to 110 °C, the control unit (C) transmits a signal to the operator of the buried site (10) by transmitting a signal to the second step Instructs to move to the phosphorus processing facility (7).

And, as the base treatment process proceeds, the temperature of the fermenter 25 of the base treatment facility 7 is measured by the second temperature sensor T2, and when a predetermined temperature is reached, it is determined whether the fermentation process is complete. do.

On the other hand, in general, odor is a very serious problem in the treatment of liquid feces of livestock, but the liquid feces treated by the ultra-high temperature fermentation process (3) of 100° C. or higher of the present invention are completely fermented with a moisture content of 25 to 35%, so there is no smell In addition, in the fermentation process by mixing the final fermentation product with the livestock liquid fecal waste again, there is an effect of removing the odor of the livestock liquid fecal waste.

The present invention has the advantage that it can be treated by mixing only the ultra-high temperature medium and livestock liquid feces, and can be fused with other organic wastes such as the ultra-high temperature medium and sewage sludge and food waste.

And, it can be confirmed through the following process whether the pathogen is killed by the livestock feces treatment system by the ultra-high temperature microorganism of the present invention.

As shown in FIG. 12 , ultra-high temperature fermentation is performed, samples are collected and sampled, and DNA and RNA are extracted from the samples. Then, after detecting the genome of the pathogen, it proceeds in the order of confirming the residual virus through TCID measurement.

To describe this process in detail, the attenuated Hepatitis A Virus (HAV) belonging to Picornaviridae that can replace foot-and-mouth disease is used, and standard pathogen selection and death are confirmed from domestic legal pathogens in addition to foot-and-mouth disease.

That is, in addition to AI and HAV, 2 types of viruses (Corona virus, Adeno virus), 5 types of bacteria (Clostiridium, Mycobacterium, Salmonella, E.coli, Basillus), and 1 type of fungus (Trycophyton) are used.

Table 2 below shows Species applicable to legal pathogens.

And, when confirming the death of pathogens in the liquid manure of livestock, it can be confirmed that 10 selected pathogens are killed by the heat generated during the ultra-high temperature fermentation process in the liquid manure.

First, 10 selected pathogens are mixed with gelatin and injected into the filter, and the filter mixed with the pathogen and gelatin is placed in a metal mesh and inserted into meat serving as an organism, followed by ultra-high temperature aerobic fermentation system.

Then, after fermentation, the filter in the metal mesh is collected, dissolved in PBS, and centrifuged at 3000 rpm to remove the solid.

Then, in order to check the remaining pathogens in the centrifuged supernatant, the virus measures TCID (tissue culture infectious dose) using the cells, and the bacteria and fungi are cultured using an appropriate medium.

At this time, as shown in FIG. 13 , in the process of confirming the residual virus through the TCID measurement, the virus in the positive control group is harvested at the time of maximum proliferation during subculture, and then the virus titer is measured.

The intracellular proliferation of the virus is checked by the presence or absence of a specific cytopathic effect (synthetic body formation), and the titer is measured by the Karber method, and the virus titer is ^{adjusted to be at least 10 6} TCID ₅₀ /ml before conducting the experiment. do.

An appropriate amount of each of the positive control group and the experimental group is inoculated into cells and the virus content is measured.

At this time, the degree of loss of the infectivity of the virus is measured using the microplate titration method, and the proliferation is confirmed by the presence or absence of a specific cytopathic effect (synysis formation) after culturing for 3 days in a _{37°C CO 2 incubator.}

On the other hand, it is also possible to check the residual bacteria using the nutrient medium, proceed as follows.

That is, the bacteria of the positive control group are inoculated into a nutrient medium and cultured with shaking at 37° C. for 22 to 26 hours to incubate the bacteria at a concentration of 10 ⁸ cfu/ml or more. Then, 1 ml of the positive control group and the experimental group were put in 5 test tubes containing a nutrient medium, mixed, and incubated at 37°C for 48 hours.

And, the disinfection effect on bacteria is judged to be effective if no growth of 4 or more in the same 5 test groups is recognized.

In addition, the genome of the residual pathogen may be identified using PCR. That is, after DNA/RNA extraction from the supernatant, PCR is performed to check whether the pathogen genome remains.

Table 3 below shows the results of the pathogen test by PCR of livestock liquid manure.

In the above experimental results, Stool showed the period of reaction with YM medium by burying feces in the fermenter, and the temperature was treated only by ultra-high temperature fermentation heat by sealing the pathogen.

As a result of the experiment, it was confirmed that all pathogens were killed.

On the other hand, Figure 16a is a graph showing the fermentation temperature during the on-site burial treatment of pig liquid manure. As shown, the pig liquid manure was buried for 21 days at the site, and the result of treatment at the base facility for 21 days was shown.

And, Figure 16b shows the results of burial treatment of pig liquid manure in the field for 18 days.

Claims (6)

A process of fermenting and decomposing livestock liquid feces by ultra-high temperature microorganisms,
The ultra-high temperature fermentation treatment process of livestock feces includes: a first step (3) of excavating a burial site (10) in an area adjacent to an outbreak site and treating liquid feces of livestock by ultra-high temperature microorganisms;
After the first process (3), it includes a second process (6) of moving the liquid feces of livestock to a base treatment facility (7) and treating it,
The buried fermentation process (3) of the first process (3) excavates the burial site 10 at the site of the outbreak, mixes livestock liquid feces and ultra-high temperature microbial medium into the burial site 10, and then excavates As a process of fermentation treatment to restore the original soil before the onset of the disease by covering the burial site (10) again with the old soil,
An impermeable material (12) is placed on the bottom and sides of the burial site (10), and a diffuser section (16) is disposed thereon;
The diffuser 16 includes an inlet pipe 18 to which compressed air is supplied; a diffuser pipe 20 connected to the inlet pipe 18 and discharging air to the outside; a branch pipe 22 connecting between the diffusers 20 and discharging air; It has a coil shape and is disposed to surround the outside of the branch pipe 22 so that when livestock feces and ultra-high temperature microbial medium are covered, a void is formed between the coil and the outer circumferential surface of the branch pipe 22, and air passes through the void to allow livestock feces and ultra-high temperature An elastic member 24 to allow penetration between the mixture of microbial media,
The ultra-high temperature microorganisms are livestock feces ultra-high temperature fermentation processing process comprising microorganisms fermenting livestock feces at 85 ~ 110 ℃ and aerobic conditions. The method of claim 1,
An ultra-high temperature fermentation process for livestock feces that prevents damage to vinyl by additionally covering the top of the impermeable material with a non-woven fabric or plastic cover. The method of claim 1,
The base treatment facility 7 includes a plurality of fermenters 25 ; a mixing tank 27 for mixing livestock liquid feces fermented in the fermenter 25 and ultra-high temperature microbial medium; Including a blower (Blower; 29) for blowing air,
Livestock feces ultra-high temperature fermentation processing process in which the waste fermented for a certain period in the fermenter 25 is transferred to the mixing tank 27 and the waste fermented for a certain period in the mixing tank 27 is transferred back to the fermenter 25 . The method of claim 1,
a first temperature sensor (T1) mounted on the burial site (10) of the buried fermentation process (3) to measure the fermentation temperature;
a second temperature sensor (T3) disposed in the fermenter 25 of the base treatment facility (7) to measure the fermentation temperature;
Livestock feces ultra-high temperature fermentation processing process further comprising a control unit (C) for determining whether each process proceeds by transmitting the temperature values measured by the first and second temperature sensors (T1, T2). delete The ultra-high temperature fermentation process for livestock feces according to claim 1, wherein the ultra-high temperature microorganism belongs to Caldothrix Satsumae, and is Caldothrix Satsumae YM081 (FERM BP-8233). .

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